CN104818213A - Sliding type closed pathogen detection card box - Google Patents
Sliding type closed pathogen detection card box Download PDFInfo
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- CN104818213A CN104818213A CN201510246613.8A CN201510246613A CN104818213A CN 104818213 A CN104818213 A CN 104818213A CN 201510246613 A CN201510246613 A CN 201510246613A CN 104818213 A CN104818213 A CN 104818213A
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- sliding type
- pathogen detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention provides a sliding type closed pathogen detection card box comprising a transparent box body, wherein the box body consists of a front wall, a rear wall and an annular enclosure; both the front wall and the rear wall are respectively provided with a circle of grooves, and two ring openings of the annular enclosure are embedded into the grooves of the front wall and the rear wall in a matching mode; the annular enclosure consists of an inner-layer annular ring and an outer-layer annular ring; the inner-layer annular ring is made of a transparent hard material, and a long elliptical hole is formed in the top surface of the inner-layer annular ring; the outer-layer annular ring is an annular transparent sheet, a round hole is formed in the sheet corresponding to the long elliptical hole, and the annular transparent sheet can slip along the inner-layer annular ring; and a base which is fixed at the bottom of the inner-layer annular ring is arranged in a cavity, a plurality of uniformly-arranged reagent tanks are formed in the base, and all reagents required for a detection process are pre-fed in the reagent tanks. According to the sliding type closed pathogen detection card box provided by the invention, a disease detection process can be completed in a basically closed environment, and the sliding type closed pathogen detection card box is disposable, so that the cross contamination problems and the infection hazards of experimenters can be greatly reduced.
Description
Technical field
The present invention relates to and a kind of detect cartridge, be specifically related to a kind of sliding type and close pathogen detection cartridge, be applicable to, in the biochemical detection system of portable, automatization, belong to biology, analytical chemistry and medical science.
Background technology
The testing process of disease, first all needs to carry out separation and purification to pathogen nucleic acid, and numerous and diverse, the time-consuming length of traditional separate nucleic acid technological step, extraction efficiency are low, can contact toxic reagent, and is carry out under an open environment, easily produces pollution.Adopt the paramagnetic particle method nucleic acid extraction technology based on magnetic separation technique just can overcome above-mentioned shortcoming well.The method do not need centrifugal, do not need to add multiple toxic reagent, safety non-toxic, fast and reliable, simple to operate.After nucleic acid extraction completes, needs carry out pcr amplification to the sample nucleic acid extracted, and finally detect.Whole process (nucleic acid extraction, pcr amplification, detects) need to operate on different instruments to carry out, sample needs transfer repeatedly, this considerably increases the probability that sample produces crossed contamination, pollute the operating process that sampling of especially uncapping after pcr amplification detects.Thus cause last detected result inaccurate, make all to bring very serious harm to patient.
Also some are had can to realize the integrated apparatus of sample preparation and test in the market, as the GeneXpert series of Cepheid company.These overwhelming majority are all external instruments, and price is very expensive, and are not suitable for promoting, and particularly hold explosive place in diseases such as Africa.For the disease that some infectivity are strong especially, such as Ebola virus, tests and carries out in enclosed environment, greatly can reduce the probability of the infection virus of experimenter.Therefore, the present invention, according to current background present situation, designs the pathogen detection cartridge of the disease that a kind of sliding type is closed, and whole testing process can be allowed to complete in closed environment, detects after terminating and directly can dispose cartridge.
Summary of the invention
The object of the invention is to provide a kind of sliding type and closes pathogen detection cartridge, and the structure of this cartridge is simple, and whole disease detection step is easy, quick, efficiency is high, with low cost, and operating environment closes safety non-pollution.
Technical scheme of the present invention is: a kind of sliding type closes pathogen detection cartridge, comprises a transparent cartridge, and described box body is made up of antetheca, rear wall and an annular enclosure; Described antetheca and rear wall staggered relatively, two opposing side edges places all have a circle groove, and annular enclosure two encloses that mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a cavity;
Described annular enclosure is made up of internal layer ring and outer ring; Described internal layer ring is rigid transparent material, end face offers an oblong hole; Described outer ring is an annular transparent thin slice, thin slice has a circular port with corresponding position, oblong hole, aperture is identical with the minor axis in oblong hole or more smaller than minor axis, described annular transparent thin slice is a bendable material, it overlays on internal layer ring outside surface, can slide along internal layer ring;
Arrange one in described cavity and be fixed on base bottom internal layer ring, base offers some evenly distributed reagent troughs, in reagent trough, be pre-loaded into all reagent that testing process needs;
Described detection cartridge is furnished with a pipettor, described pipettor passes the circular port of outer ring above reagent trough, moved left and right within the scope of oblong hole along internal layer ring by outer ring, realize the application of sample between different reagent trough and move liquid, completing whole disease detection process.
Further, the material of described reagent trough is inertia and non electrically conductive material, all chemical reaction does not occur with all agent formulations.
Further, described internal layer ring is PP material.High and the optical clear of heat transfer efficiency.
Further, described antetheca and rear wall material are hard transparent plastic.Cost is low, simply easy to make.
Further, the gap sealing-ring that described annular enclosure two is enclosed between mouth and groove seals, and makes whole cavity form substantially airtight space.
The present invention also provides a kind of sliding type to close the loading methods of pathogen detection cartridge, the pipettor with fluid suction head is utilized to draw sample to be tested, enter in cartridge cavity from the circular port of outer ring, pipettor is moved left and right within the scope of oblong hole, sample is injected in corresponding reagent trough, then blow and beat mixing, coordinate peripheral equipment to react.
What the present invention also provided a kind of sliding type to close pathogen detection cartridge moves liquid method, utilize and draw liquid to be moved with the pipettor of fluid suction head, within the scope of oblong hole, move left and right correct reagent trough position by inhaling the pipettor needing to be moved liquid, liquid to be moved is injected in reagent trough.
Advantage of the present invention:
Cartridge cost of the present invention is low, and structure is simple, easy to operate, and is easy to integration realization automatization.Disease detection process completes in the environment of base closed, and single use, greatly reduces the infection harm of cross-contamination issue and experimenter.External instrument device required in detection greatly reduces.
Accompanying drawing explanation
Fig. 1 cartridge overall appearance figure;
The inner upward view of Fig. 2;
Fig. 3 sectional structure chart.
Wherein: base 11, reagent trough 12, outer ring 13, internal layer ring 14, application of sample moves fluid apertures (circular port that outer ring is offered) 15, elongated hole 16, groove 17, antetheca 18, rear wall 19.
Embodiment
Below in conjunction with embodiment and accompanying drawing 1,2,3 couples of the present invention do and further explain.The following example only for illustration of the present invention, but is not used for limiting practical range of the present invention.
A kind of sliding type of the present invention closes pathogen detection cartridge, and comprise a transparent cartridge, described box body is made up of antetheca 18, rear wall 19 and an annular enclosure.Described antetheca 18 and rear wall 19 are hard transparent plastic, staggered relatively, and two opposing side edges places all have a circle groove 17, and annular enclosure two encloses that mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a cavity.The gap sealing-ring that described annular enclosure two is enclosed between mouth and groove seals, and makes whole cavity form substantially airtight space.
Described annular enclosure is made up of internal layer ring 14 and outer ring 13; Described internal layer ring 14 is rigid transparent material, heat transfer efficiency can be selected high and optically transparent PP material, end face offer an oblong hole.Described outer ring 13 is an annular transparent thin slice, thin slice has a circular port 15 with corresponding position, oblong hole, aperture is identical with the minor axis in oblong hole or more smaller than minor axis, described annular transparent thin slice is a bendable material, it overlays on internal layer ring outside surface, can slide along internal layer ring.
Arrange one in described cavity and be fixed on base 11 bottom internal layer ring, base offers some evenly distributed reagent troughs 12, for nucleic acid extraction, amplification and detecting.The material of described reagent trough is inertia and non electrically conductive material, all chemical reaction does not occur with all agent formulations.All reagent that testing process needs are pre-loaded in reagent trough 12; As lysate, scavenging solution, elutriant, the damping fluid needed for amplification, dNTP, enzyme, primer, probe and prevent reagent to be subject to the mineral wet goods of thermal evaporation.
Described detection cartridge is furnished with a pipettor, described pipettor passes the circular port of outer ring above reagent trough, moved left and right within the scope of oblong hole along internal layer ring by outer ring, realize the application of sample between different reagent trough and move liquid, completing whole disease detection process.
Application of sample process: utilize the pipettor with fluid suction head to draw sample to be tested, enter in cartridge cavity from the circular port of outer ring, pipettor is moved left and right within the scope of oblong hole, sample is injected in corresponding reagent trough, then mixing is blown and beaten, coordinate outside heating, inhale the equipment such as magnetic and react.
Move liquid process: utilize the pipettor with fluid suction head to draw liquid to be moved, within the scope of oblong hole, move left and right correct reagent trough position by inhaling the pipettor needing to be moved liquid, liquid to be moved is injected in reagent trough.
Whole testing process only needs the liquid-transfering gun of a band fluid suction head, then coordinates outside corresponding function device, and just can complete the detection of pathogenic agent in the environment of base closed, experimental implementation is simple, safety.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple equivalents to technical scheme of the present invention, these equivalents all belong to protection scope of the present invention.
Claims (7)
1. sliding type closes a pathogen detection cartridge, it is characterized in that: comprise a transparent cartridge, and described box body is made up of antetheca, rear wall and an annular enclosure; Described antetheca and rear wall staggered relatively, two opposing side edges places all have a circle groove, and annular enclosure two encloses that mouth is supporting to be embedded in the groove of antetheca and rear wall, forms a cavity;
Described annular enclosure is made up of internal layer ring and outer ring; Described internal layer ring is rigid transparent material, end face offers an oblong hole; Described outer ring is an annular transparent thin slice, thin slice has a circular port with corresponding position, oblong hole, aperture is identical with the minor axis in oblong hole or more smaller than minor axis, described annular transparent thin slice is a bendable material, it overlays on internal layer ring outside surface, can slide along internal layer ring;
Arrange one in described cavity and be fixed on base bottom internal layer ring, base offers some evenly distributed reagent troughs, in reagent trough, be pre-loaded into all reagent that testing process needs;
Described detection cartridge is furnished with a pipettor, described pipettor passes the circular port of outer ring above reagent trough, moved left and right within the scope of oblong hole along internal layer ring by outer ring, realize the application of sample between different reagent trough and move liquid, completing whole disease detection process.
2. a kind of sliding type according to claim 1 closes pathogen detection cartridge, it is characterized in that: the material of described reagent trough is inertia and non electrically conductive material, all chemical reaction does not occur with all agent formulations.
3. a kind of sliding type according to claim 1 closes pathogen detection cartridge, it is characterized in that: described internal layer ring is PP material.
4. a kind of sliding type according to claim 1 closes pathogen detection cartridge, it is characterized in that: described antetheca and rear wall material are hard transparent plastic.
5. a kind of sliding type according to claim 1 closes pathogen detection cartridge, it is characterized in that: the gap sealing-ring that described annular enclosure two is enclosed between mouth and groove seals.
6. a sliding type closes the loading methods of pathogen detection cartridge, it is characterized in that: utilize the pipettor with fluid suction head to draw sample to be tested, enter in cartridge cavity from the circular port of outer ring, pipettor is moved left and right within the scope of oblong hole, sample is injected in corresponding reagent trough, then blow and beat mixing, coordinate peripheral equipment to react.
7. what a sliding type closed pathogen detection cartridge moves liquid method, it is characterized in that: utilize the pipettor with fluid suction head to draw liquid to be moved, within the scope of oblong hole, move left and right correct reagent trough position by inhaling the pipettor needing to be moved liquid, liquid to be moved is injected in reagent trough.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510246613.8A CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510246613.8A CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
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| Publication Number | Publication Date |
|---|---|
| CN104818213A true CN104818213A (en) | 2015-08-05 |
| CN104818213B CN104818213B (en) | 2017-02-22 |
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| CN201510246613.8A Active CN104818213B (en) | 2015-05-14 | 2015-05-14 | Sliding type closed pathogen detection card box |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586256A (en) * | 2016-02-24 | 2016-05-18 | 东南大学 | Closed pathogen detection cassette |
| CN106526202A (en) * | 2016-11-17 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Enclosed pathogen detection kit |
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN110404594A (en) * | 2019-07-31 | 2019-11-05 | 深圳麦科田生物医疗技术有限公司 | Sample analysis kit |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426984Y (en) * | 2000-06-07 | 2001-04-18 | 中国水产科学研究院黄海水产研究所 | Prawn pathogeny detecting reagent box |
| US20070116613A1 (en) * | 2005-11-23 | 2007-05-24 | Donat Elsener | Sample tube and system for storing and providing nucleic acid samples |
| US20120329143A1 (en) * | 2011-06-27 | 2012-12-27 | Hitachi High-Technologies Corporation | Chilled Reagent Container and Nucleic Acid Analyzer |
| CN204702755U (en) * | 2015-05-14 | 2015-10-14 | 东南大学 | A kind of sliding type closes pathogen detection cartridge |
-
2015
- 2015-05-14 CN CN201510246613.8A patent/CN104818213B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426984Y (en) * | 2000-06-07 | 2001-04-18 | 中国水产科学研究院黄海水产研究所 | Prawn pathogeny detecting reagent box |
| US20070116613A1 (en) * | 2005-11-23 | 2007-05-24 | Donat Elsener | Sample tube and system for storing and providing nucleic acid samples |
| US20120329143A1 (en) * | 2011-06-27 | 2012-12-27 | Hitachi High-Technologies Corporation | Chilled Reagent Container and Nucleic Acid Analyzer |
| CN204702755U (en) * | 2015-05-14 | 2015-10-14 | 东南大学 | A kind of sliding type closes pathogen detection cartridge |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586256A (en) * | 2016-02-24 | 2016-05-18 | 东南大学 | Closed pathogen detection cassette |
| CN105586256B (en) * | 2016-02-24 | 2017-11-03 | 东南大学 | A kind of closed pathogen detection cartridge |
| CN106526202A (en) * | 2016-11-17 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Enclosed pathogen detection kit |
| CN106526202B (en) * | 2016-11-17 | 2018-07-13 | 百奥森（江苏）食品安全科技有限公司 | A kind of closed pathogen detection cartridge |
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN110404594A (en) * | 2019-07-31 | 2019-11-05 | 深圳麦科田生物医疗技术有限公司 | Sample analysis kit |
| CN110404594B (en) * | 2019-07-31 | 2024-06-04 | 深圳麦科田生物医疗技术股份有限公司 | Sample analysis kit |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
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| Publication number | Publication date |
|---|---|
| CN104818213B (en) | 2017-02-22 |
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