CN105586256A - Closed pathogen detection cassette - Google Patents
Closed pathogen detection cassette Download PDFInfo
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- CN105586256A CN105586256A CN201610102868.1A CN201610102868A CN105586256A CN 105586256 A CN105586256 A CN 105586256A CN 201610102868 A CN201610102868 A CN 201610102868A CN 105586256 A CN105586256 A CN 105586256A
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- cavity
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- cylindrical sleeve
- slide plate
- way connector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a closed pathogen detection cassette which comprises a cavity, a sliding blade arranged on the closed cavity, a pipette assembly combined with the sliding blade, a button lid, and a pedestal which is matched and combined with the cavity. The cassette can complete the whole pathogen detection process (including nucleic acid extraction, amplification and detection) in a closed environment, and is suitable for automatic control. The cassette can effectively avoid the problem of pollution in amplification detection of pathogenic microorganisms and the like, the problem of infection of the experiment operators, and other problems.
Description
Technical field
The present invention relates to a kind of closed pathogen detection cartridge, be applicable to, in the biochemistry detection system of portable, automation, belong toIn biology, analytical chemistry and medical science detection field.
Background technology
Detection of nucleic acids has been widely used in the fields such as biological medicine. The whole process of traditional detection of nucleic acids (comprises that nucleic acid carriesGet, pcr amplification, detects) need on different instruments, operate and carry out, sample need to shift repeatedly, and this has increased sample greatlyProduct produce the probability of cross pollution, in the operating process that the sampling of especially uncapping from pcr amplification detects, pollute. Thereby leadCause last testing result inaccurate, make patient all bring very serious harm. Although developed serial of methodsPrevent the cross pollution of amplified production, as PCR operation carried out to strict subregion control, adopt stopped pipe amplification and the method detecting,Utilize the method for enzyme solution pollution abatement etc., but these methods have relatively high expectations to experiment condition and operating personnel, and artificially because ofElement impact is larger. Also the integrated apparatus that has in the market some can realize sample preparation and detection, as CepheidThe GeneXpert series of company. These overwhelming majority are all external instruments, and price is very expensive, and are not suitable for promoting spyNot not to hold explosive place in diseases such as Africa.
Summary of the invention
Goal of the invention: the object of the invention is to provide a kind of portable, easy to operate, is applicable to full automatic closed pathogen detectionCartridge.
Technical scheme: object of the present invention is achieved through the following technical solutions:
A kind of closed detection cartridge, comprises a cavity, at cavity top, buckle closure is set, and closed cavity is set on buckle closureSlide plate and be combined with slide plate move liquid assembly, the base that mates combination with cavity is set, described cavity front and back inwall in cavity bottomOn be provided for U-shaped guide groove and the outstanding rectangular beam that described slide plate slides, described in the spacing that two outstanding rectangular beams form can limit, moveLiquid assembly steadily moves, and before and after described cavity, inwall lower end is also provided with rectangular groove, respectively has a button hole simultaneously.
Described slide plate is provided with manhole.
At least two oblique shallow slots of the each setting of left and right lateral wall of described cavity.
The described liquid assembly that moves comprises the two-way connector that connects imbibition head and plunger displacement pump pipeline, spring, cylindrical sleeve, cylinderShape gland; Described spring is in cylindrical sleeve, and two-way connector inserts in spring, and cylindrical gland is pushed down two-way connector,Spring is limited in cylindrical sleeve; The described two-way connector upper end of moving in liquid assembly is tower head shape, with the pipe of plunger displacement pumpRoad UNICOM, there is certain taper lower end, coordinates with imbibition head, and described cylindrical sleeve inwall is arranged with two guide grooves, describedThe symmetrical both sides of two-way connector are provided with outstanding top guide, coordinate with the guide groove of cylindrical sleeve inwall, described in move the cylinder in liquid assemblyShape sleeve is through the through hole on described slide plate, and the catching groove of described cylindrical sleeve both sides fastens the snap-on of cylindrical gland both sides, willSlide plate is pressed abd fixed between cylindrical sleeve and cylindrical gland.
Some circular holes not of uniform size are set for laying the centrifuge tube of different size on described base, on base inner edge, have someCatching groove, described base two foreign minister limits are also respectively provided with outstanding rectangular and prominent button, under described outstanding rectangular and prominent button and cavity front and back inwallThe rectangular groove of end and button hole coordinate.
Cartridge according to claim 1, is characterized in that: also comprise base compressing tablet, described base compressing tablet is arranged on baseInside is provided with the hole corresponding with circular hole position on described base on base compressing tablet, and the aperture in described hole is slightly less than circular hole on baseAperture, compressing tablet edge arranges the prominent button corresponding with catching groove position on base inner edge.
Beneficial effect: the whole process that pathogen detection cartridge of the present invention can collect pathogen detection comprises nucleic acid extraction, expandsIncrease, be detected in the environment of sealing and complete, be applicable to automation control. Can effectively avoid depositing in the augmentation detection such as pathogenic microorganismThe problem such as pollution problem and the infection of experiment operator.
Brief description of the drawings
Fig. 1 cartridge overall appearance figure;
Fig. 2 cartridge profile;
Fig. 3 a cartridge left view;
Fig. 3 b cartridge top view;
Fig. 4 cartridge is moved liquid assembly assumption diagram;
Fig. 5 cartridge understructure figure.
Wherein: closed pathogen detection cartridge 1, slide plate 101, cavity 102, buckle closure 103, base 104, moves liquid assembly105, centrifuge tube 106, U-shaped guide groove 201, outstanding rectangular beam 202, tiltedly shallow slot 203, rectangular groove 204, cavity button hole 205,The prominent button 301 of buckle closure, base compressing tablet 401, base circular hole 402, compressing tablet circular hole 403, base catching groove 404, the prominent button 405 of compressing tablet,Base is outstanding rectangular 406, the prominent button 407 of base, two-way connector 501, cylindrical sleeve 502, cylindrical gland 503, bulletSpring 504, tower head 505, imbibition 506, outstanding top guide 507
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is done further and explained. The following example is only for the present invention is described, but is not used forLimit practical range of the present invention.
The closed pathogen detection cartridge 1 of one the present invention relates to, mainly by slide plate 101, cavity 102, buckle closure 103, the endSeat 104, moves liquid assembly 105, and centrifuge tube 106 forms, and moves in liquid assembly 105, by moving described in described slide plate 101 is fixed onThe mobile slide plate 101 that drives of liquid assembly 105 moves along the U-shaped guide groove 201 in cavity 102, moves the top of liquid assembly 105Fasten cavity 102 and vertical direction is fixed in cavity 102 by the buckle closure button 301 of dashing forward with buckle closure 103, horizontal direction canOutstanding rectangular beam 202 along cavity 102 upper edge edges and its inside moves. Centrifuge tube 106 is put into base 104 after cutting off upper coverIn base circular hole 402 in, base compressing tablet 401 405 fastens base catching groove 404 by the compressing tablet button of dashing forward, and makes base compressing tablet 401Centrifuge tube 106 is fixed in base circular hole to 402. Base circular hole 402, compressing tablet circular hole 403, centrifuge tube pipe diameter size adaptation.In centrifuge tube 106, add required reagent by compressing tablet circular hole 403, available masking foil seals all compressing tablets of base compressing tablet 401Circular hole 403, avoids the pollution of reagent. Then base 104 is given prominence to rectangular 406 length along cavity 102 inwalls by baseBar groove 204 is pushed in cavity, and the prominent button 407 of base buckles in cavity button hole 205, and base 104 and cavity 102 are tiedClose. Like this, slide plate 101, cavity 102, buckle closure 103, base 104, moves liquid assembly 105, and centrifuge tube 106 just forms envelopeThe environment closing.
What the detection of pathogen mainly needed realization in closed cartridge is the operations such as mixing of liquid, transfer. Control by outsideSystem presses down the two-way connector 501 in liquid assembly 105 that moves of closed pathogen detection cartridge 1, and two-way connector 501 passesCylindrical gland 503 Compress Springs 504, the outstanding top guide 507 of two-way connector 501 is along cylindrical sleeve 502 inwallsGuide groove moves down, and in the imbibition of two-way connector 501 lower ends 506 centrifuge tube 106 that can enter in base 104, controlsThe imbibition of peripheral hardware plunger displacement pump or tapping, can realize imbibition and tapping in centrifuge tube 106. When imbibition 506 liquid of drawingNeed to shift time, remove the downforce of two-way connector 501, spring 504 screen resiliences of compression are with two-way connector 501To inhale has the imbibition of liquid 506 to move up, and spring 504 restores. Control moves liquid component level and moves to destination locations, heavyMultiple above-mentioned operation, can realize the operation that moves liquid. Control the high frequency of outside plunger displacement pump and inhale tapping, can realize in centrifuge tubeTo liquid, piping and druming mixes operation. Other operations of pathogen detection can realize by external drive. To sum up, coordinate external drive,Can complete imbibition in enclosed pathogen detection cartridge, tapping, moves liquid, and piping and druming such as mixes at the action, realizes the detection of pathogen.
More than describe the preferred embodiment of the present invention in detail, still, the present invention is not limited to concrete in above-mentioned embodimentDetails, within the scope of technical conceive of the present invention, can carry out multiple equivalents to technical scheme of the present invention, and these are equal toConversion all belongs to protection scope of the present invention.
Claims (6)
1. a closed detection cartridge, it is characterized in that: comprise a cavity, at cavity top, buckle closure is set, the slide plate of closed cavity is set on buckle closure and be combined with slide plate move liquid assembly, the base that mates combination with cavity is set in cavity bottom, before and after described cavity, on inwall, be provided for U-shaped guide groove and outstanding rectangular beam that described slide plate slides, described in the spacing that two outstanding rectangular beams form can limit, moving liquid assembly steadily moves, before and after described cavity, inwall lower end is also provided with rectangular groove, respectively has a button hole simultaneously.
2. cartridge according to claim 1, is characterized in that: described slide plate is provided with manhole.
3. cartridge according to claim 1, is characterized in that: at least two oblique shallow slots of the each setting of left and right lateral wall of described cavity.
4. cartridge according to claim 1, is characterized in that: the described liquid assembly that moves comprises the two-way connector that connects imbibition head and plunger displacement pump pipeline, spring, cylindrical sleeve, cylindrical gland; Described spring is in cylindrical sleeve, and two-way connector inserts in spring, and cylindrical gland is pushed down two-way connector, and spring is limited in cylindrical sleeve; The described two-way connector upper end of moving in liquid assembly is tower head shape, pipeline UNICOM with plunger displacement pump, there is certain taper lower end, coordinate with imbibition head, described cylindrical sleeve inwall is arranged with two guide grooves, the symmetrical both sides of described two-way connector are provided with outstanding top guide, coordinate with the guide groove of cylindrical sleeve inwall, the described cylindrical sleeve moving in liquid assembly passes the through hole on described slide plate, the catching groove of described cylindrical sleeve both sides fastens the snap-on of cylindrical gland both sides, and slide plate is pressed abd fixed between cylindrical sleeve and cylindrical gland.
5. cartridge according to claim 1, it is characterized in that: some circular holes not of uniform size are set for laying the centrifuge tube of different size on described base, on base inner edge, there are some catching grooves, described base two foreign minister limits are also respectively provided with outstanding rectangular and prominent button, and described outstanding rectangular and prominent button coordinates with rectangular groove and the button hole of inwall lower end before and after cavity.
6. cartridge according to claim 1, it is characterized in that: also comprise base compressing tablet, described base compressing tablet is arranged on base inside, on base compressing tablet, be provided with the hole corresponding with circular hole position on described base, the aperture in described hole is slightly less than the aperture of circular hole on base, and compressing tablet edge arranges the prominent button corresponding with catching groove position on base inner edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102868.1A CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610102868.1A CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
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| Publication Number | Publication Date |
|---|---|
| CN105586256A true CN105586256A (en) | 2016-05-18 |
| CN105586256B CN105586256B (en) | 2017-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610102868.1A Active CN105586256B (en) | 2016-02-24 | 2016-02-24 | A kind of closed pathogen detection cartridge |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106318865A (en) * | 2016-10-25 | 2017-01-11 | 西安交通大学 | Portable device for nucleic acid extraction and gene amplification |
| CN106353492A (en) * | 2016-10-13 | 2017-01-25 | 东南大学 | Cassette device for screening tumor cell aptamers |
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120329143A1 (en) * | 2011-06-27 | 2012-12-27 | Hitachi High-Technologies Corporation | Chilled Reagent Container and Nucleic Acid Analyzer |
| CN203663858U (en) * | 2013-11-26 | 2014-06-25 | 东南大学 | Multi-channel pipette |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN205382165U (en) * | 2016-02-24 | 2016-07-13 | 东南大学 | Closed pathogen detection card box |
-
2016
- 2016-02-24 CN CN201610102868.1A patent/CN105586256B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120329143A1 (en) * | 2011-06-27 | 2012-12-27 | Hitachi High-Technologies Corporation | Chilled Reagent Container and Nucleic Acid Analyzer |
| CN203663858U (en) * | 2013-11-26 | 2014-06-25 | 东南大学 | Multi-channel pipette |
| CN104818213A (en) * | 2015-05-14 | 2015-08-05 | 东南大学 | Sliding type closed pathogen detection card box |
| CN205382165U (en) * | 2016-02-24 | 2016-07-13 | 东南大学 | Closed pathogen detection card box |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353492A (en) * | 2016-10-13 | 2017-01-25 | 东南大学 | Cassette device for screening tumor cell aptamers |
| CN106318865A (en) * | 2016-10-25 | 2017-01-11 | 西安交通大学 | Portable device for nucleic acid extraction and gene amplification |
| CN106318865B (en) * | 2016-10-25 | 2018-03-16 | 西安交通大学 | A kind of nucleic acid extraction and the portable unit of gene magnification |
| CN107964504A (en) * | 2017-10-28 | 2018-04-27 | 深圳职业技术学院 | A kind of closed sample integrity detection device and detection method |
| CN107964504B (en) * | 2017-10-28 | 2023-10-27 | 深圳职业技术学院 | Closed sample integrated detection device and detection method |
| CN113150945A (en) * | 2021-04-14 | 2021-07-23 | 新羿制造科技（北京）有限公司 | Slide type cassette for nucleic acid extraction and PCR amplification detection |
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| Publication number | Publication date |
|---|---|
| CN105586256B (en) | 2017-11-03 |
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