CN111826281A - Nucleic acid detects composite module - Google Patents
Nucleic acid detects composite module Download PDFInfo
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- CN111826281A CN111826281A CN202010734240.XA CN202010734240A CN111826281A CN 111826281 A CN111826281 A CN 111826281A CN 202010734240 A CN202010734240 A CN 202010734240A CN 111826281 A CN111826281 A CN 111826281A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention provides a nucleic acid detection combined module, and relates to the technical field of nucleic acid detection. This nucleic acid detects composite module, including unable adjustment base, one side at unable adjustment base top is provided with reagent and prepares the device, one side that reagent prepared the device is provided with nucleic acid extraction element, one side that nucleic acid extraction element kept away from reagent and prepared the device is provided with the nucleic acid amplification device, one side that nucleic acid extraction element was kept away from to the nucleic acid amplification device is provided with waste treatment device. This nucleic acid detects composite module prepares the device, nucleic acid extraction element, nucleic acid amplification device and waste treatment device through setting up the reagent, can prepare the reagent, nucleic acid extraction, nucleic acid extension and waste treatment accomplish in the airtight space of cutting apart one by one the inside, and this composite module, small in addition, compatible current extraction and amplification equipment can realize doing infection class nucleic acid detection in a room or carriage, thereby make its work efficiency improve greatly, be worth wideling popularize.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid detection combined module.
Background
Nucleic acid detection is an important detection mode for virus detection, but the existing nucleic acid detection equipment and detection mode have great defects, the original detection method can only be carried out in a standard laboratory, generally 3 laboratories and a series of matching implementation are needed for detection, not only can a large amount of space be occupied, but also a complete detection process can be completed by matching a large amount of personnel, so that the detection efficiency is low, and the detection is very inconvenient. Without a P2-rated laboratory, the operator can detect coronavirus without P3 protection.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a combined module for nucleic acid detection, which solves the problems that the existing nucleic acid detection equipment and detection mode are relatively large, the original detection method can be only carried out in a standard laboratory, generally 3 laboratories and a series of matched implementation are needed for detection, a large amount of space is occupied, and a large amount of personnel are needed for matching to complete a complete detection process, so that the detection efficiency is relatively low and the detection is very inconvenient.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a nucleic acid detection combined module comprises a fixed base, wherein a reagent preparation device is arranged on one side of the top of the fixed base, a nucleic acid extraction device is arranged on one side of the reagent preparation device, a nucleic acid amplification device is arranged on one side, away from the reagent preparation device, of the nucleic acid extraction device, and a waste treatment device is arranged on one side, away from the nucleic acid extraction device, of the nucleic acid amplification device.
Preferably, the reagent preparing device comprises a reagent preparing glass cabinet, a first transmission window and a second transmission window are respectively connected to two sides of the bottom of the front end of the reagent preparing glass cabinet in a rotating mode, an operation platform is arranged at the center of the reagent preparing glass cabinet, a detection kit is arranged at the top of the operation platform, an operation frame is arranged at the rear end of the center of the reagent preparing glass cabinet, a plurality of test tubes are arranged at the top of the operation frame, pipettors corresponding to the detection kit are arranged on one side of the plurality of test tubes, two first operation holes are formed in the center of the front end of the reagent preparing glass cabinet, and a first central control platform is installed on one side of the top of the front end of the reagent preparing glass cabinet.
Preferably, the nucleic acid extraction device includes nucleic acid extraction glass cabinet, one side of reagent preparation device is provided with nucleic acid extraction glass cabinet, the both sides of nucleic acid extraction glass cabinet front end bottom rotate respectively and are connected with third pass-through box and fourth pass-through box, the center of nucleic acid extraction glass cabinet is provided with full-automatic nucleic acid extraction appearance, the center of nucleic acid extraction glass cabinet front end is provided with the first access panel that corresponds with full-automatic nucleic acid extraction appearance, two second handle holes have been seted up at the center of first access panel, control platform in the second that corresponds with full-automatic nucleic acid extraction appearance is installed to one side at nucleic acid extraction glass cabinet front end top.
Preferably, the nucleic acid amplification device comprises a nucleic acid amplification glass window, one side of the nucleic acid extraction device, which is far away from the reagent preparation device, is provided with the nucleic acid amplification glass window, the two sides of the bottom of the front end of the nucleic acid amplification glass window are respectively and rotatably connected with a fifth transmission window and a sixth transmission window, the center of the nucleic acid amplification glass window is provided with a PCR instrument, the center of the front end of the nucleic acid amplification glass window is provided with a second access window corresponding to the PCR instrument, the center of the second access window is provided with two third operation holes corresponding to the PCR instrument, and one side of the top of the front end of the nucleic acid amplification glass window is provided with a third central control platform.
Preferably, the refuse treatment device includes the refuse treatment glass window, one side that nucleic acid extraction element was kept away from to the nucleic acid amplification device is provided with the refuse treatment glass window, one side of refuse treatment glass window front end bottom is rotated and is connected with the seventh pass-through box, the center of refuse treatment glass window is provided with first waste liquid treatment bucket and second waste liquid treatment bucket respectively, one side that the seventh pass-through box was kept away from to the front end of refuse treatment glass window is provided with the third access panel, two fourth handle holes corresponding with first waste liquid treatment bucket and second waste liquid treatment bucket have been seted up at the center of third access panel, accuse platform in the fourth is installed to one side at refuse treatment glass window front end top.
Preferably, the reagent preparation glass cabinet, the nucleic acid extraction glass cabinet, the nucleic acid amplification glass window and the waste treatment glass window are all made of transparent toughened glass materials.
Preferably, the first access panel, the second access panel and the third access panel are all arranged in a rectangular structure.
The working principle is as follows: when the nucleic acid detection combined module is used for detecting virus nucleic acid, a detection kit 205 and a related operation frame 206 are arranged at the center of a reagent preparation device 2, the detection kit 205 can be used for preparing reagents for collected virus samples, then the samples are put in a constant temperature box for inactivation for a period of time, then the inactivated samples are added into a detection orifice plate, a first central control platform 210 can display information such as pressure, temperature, humidity and time in a glass cabinet and various electrical switch controls, a nucleic acid extraction device 3 is used for extracting nucleic acid from the collected samples, after the samples are prepared, the detection orifice plate is put into a full-automatic nucleic acid extraction instrument 304 in a nucleic acid extraction device 3 for extracting nucleic acid, after the nucleic acid extraction is finished, the nucleic acid solution is added into a corresponding PCR tube, then the PCR tube filled with the nucleic acid solution is put into a PCR instrument 404 in a nucleic acid amplification device 4 for final detection, after the detection is completed, the waste liquid may be collected and disposed of by the waste disposal device 5.
(III) advantageous effects
The invention provides a nucleic acid detection combined module. The method has the following beneficial effects:
1. this nucleic acid detects composite module through the simple composite module of design, makes operating personnel operate through remote control or anti-pollution gloves outside the laboratory, can deploy fast, need not the laboratory of P2 grade, and operating personnel need not the protection of P3 can with detect coronavirus and detect.
2. This nucleic acid detects composite module prepares the device, nucleic acid extraction element, nucleic acid amplification device and waste treatment device through setting up the reagent, can prepare the reagent, nucleic acid extraction, nucleic acid extension and waste treatment accomplish in the airtight space the inside of cutting apart one by one, and this composite module, small in addition, compatible current extraction and amplification equipment can realize having done infection class nucleic acid detection in a room or carriage, thereby make its work efficiency improve greatly, be worth wideling popularize.
Drawings
FIG. 1 is a front view structural diagram of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is a schematic view of the structure of the reagent preparing apparatus according to the present invention;
FIG. 4 is a schematic view showing the construction of the nucleic acid isolation apparatus according to the present invention;
FIG. 5 is a schematic view showing the construction of a nucleic acid amplification apparatus according to the present invention;
fig. 6 is a schematic view showing the construction of the waste treatment apparatus of the present invention.
Wherein, 1, fixing the base; 2. a reagent preparing device; 201. preparing a reagent glass cabinet; 202. a first transmission window; 203. a second pass-through window; 204. an operating platform; 205. a detection kit; 206. an operation frame; 207. a test tube; 208. a pipettor; 209. a first operation hole; 210. a first central control platform; 3. A nucleic acid extraction device; 301. a nucleic acid extraction glass cabinet; 302. a third pass-through window; 303. a fourth transmission window; 304. a full-automatic nucleic acid extractor; 305. a first access panel; 306. a second operation hole; 307. a second central control platform; 4. a nucleic acid amplification device; 401. a nucleic acid amplification glass window; 402. a fifth pass-through window; 403. A sixth pass-through window; 404. a PCR instrument; 405. a second access panel; 406. a third operation hole; 407. a third central control platform; 5. a waste disposal device; 501. a waste management glazing; 502. a seventh pass-through window; 503. a first waste liquid treatment tank; 504. a second waste liquid treatment barrel; 505. a third access panel; 506. a fourth operation hole; 507. and a fourth central control platform.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
as shown in FIGS. 1 to 6, the embodiment of the present invention provides a combined module for nucleic acid detection, which comprises a fixed base 1, a reagent preparing device 2 is disposed on one side of the top of the fixed base 1, a nucleic acid extracting device 3 is disposed on one side of the reagent preparing device 2, a nucleic acid amplifying device 4 is disposed on one side of the nucleic acid extracting device 3 away from the reagent preparing device 2, a waste disposal device 5 is disposed on one side of the nucleic acid amplifying device 4 away from the nucleic acid extracting device 3, the reagent preparing device 2, the nucleic acid extracting device 3, the nucleic acid amplifying device 4 and the waste disposal device 5 can be disposed separately or combined, and the connecting doors can be fixedly connected through magnets or fixing screws by installing the connecting doors between the reagent preparing device 2, the nucleic acid extracting device 3, the nucleic acid amplifying device 4 and the waste disposal device 5, the reagent preparing device 2, the nucleic acid extracting device 3, the nucleic acid amplifying device 4 and the waste disposal device 5 can be internally provided with a pressure adjusting device, so that the internal and external pressures of the device can be always kept consistent, and meanwhile, ultraviolet disinfection lamps can be arranged on the inner surfaces of the tops of the reagent preparing device 2, the nucleic acid extracting device 3, the nucleic acid amplifying device 4 and the waste disposal device 5, so that the disinfection effect is realized, and the operation can be carried out by utilizing a special manipulator in the process of preparing, extracting or amplifying related reagents, so that the protection of testers is realized.
The reagent preparing device 2 comprises a reagent preparing glass cabinet 201, two sides of the bottom of the front end of the reagent preparing glass cabinet 201 are respectively connected with a first transmission window 202 and a second transmission window 203 in a rotating mode, an operation platform 204 is arranged in the center of the reagent preparing glass cabinet 201, a detection reagent box 205 is arranged on the top of the operation platform 204, an operation frame 206 is arranged at the rear end of the center of the reagent preparing glass cabinet 201, a plurality of test tubes 207 are arranged on the top of the operation frame 206, pipettors 208 corresponding to the detection reagent box 205 are arranged on one side of the plurality of test tubes 207, two first operation holes 209 are formed in the center of the front end of the reagent preparing glass cabinet 201, a first central control platform 210 is arranged on one side of the top of the front end of the reagent preparing glass cabinet 201, a detection reagent box 205 and a related operation frame 206 are arranged in the center of the reagent preparing device 2, and the reagent box, the sample is then inactivated in an incubator for a period of time, and then the inactivated sample is added to the detection well plate, and the first central control platform 210 can display information such as pressure, temperature, humidity, time and the like in the glass cabinet and various electrical switch controls.
The nucleic acid extraction device 3 comprises a nucleic acid extraction glass cabinet 301, one side of the reagent preparation device 2 is provided with the nucleic acid extraction glass cabinet 301, two sides of the bottom of the front end of the nucleic acid extraction glass cabinet 301 are respectively and rotatably connected with a third transfer window 302 and a fourth transfer window 303, the center of the nucleic acid extraction glass cabinet 301 is provided with a full-automatic nucleic acid extractor 304, the center of the front end of the nucleic acid extraction glass cabinet 301 is provided with a first access window 305 corresponding to the full-automatic nucleic acid extractor 304, the center of the first access window 305 is provided with two second operation holes 306, one side of the top of the front end of the nucleic acid extraction glass cabinet 301 is provided with a second central control platform 307 corresponding to the full-automatic nucleic acid extractor 304, the nucleic acid extraction device 3 is used for extracting nucleic acid from a sample, after the sample is prepared, then the detection well plate is placed in a full-automatic nucleic acid extractor 304 in the nucleic acid extracting device 3 for nucleic acid extraction.
The nucleic acid amplification device 4 comprises a nucleic acid amplification glass window 401, a nucleic acid amplification glass window 401 is arranged on one side of the nucleic acid extraction device 3 away from the reagent preparation device 2, a fifth transmission window 402 and a sixth transmission window 403 are respectively connected to two sides of the bottom of the front end of the nucleic acid amplification glass window 401 in a rotating manner, a PCR instrument 404 is arranged in the center of the nucleic acid amplification glass window 401, a second inspection window 405 corresponding to the PCR instrument 404 is arranged in the center of the front end of the nucleic acid amplification glass window 401, two third operation holes 406 corresponding to the PCR instrument 404 are formed in the center of the second inspection window 405, a third central control platform 407 is arranged on one side of the top of the front end of the nucleic acid amplification glass window 401, after the nucleic acid extraction is completed, the nucleic acid solution is added into the corresponding PCR tube, and the PCR tube filled with the nucleic acid solution is placed into the PCR instrument 404 in the nucleic acid amplification device 4 for final detection.
The waste treatment device 5 comprises a waste treatment glass window 501, one side of the nucleic acid amplification device 4, which is far away from the nucleic acid extraction device 3, is provided with the waste treatment glass window 501, one side of the bottom of the front end of the waste treatment glass window 501 is rotatably connected with a seventh transmission window 502, the center of the waste treatment glass window 501 is respectively provided with a first waste liquid treatment barrel 503 and a second waste liquid treatment barrel 504, one side of the front end of the waste treatment glass window 501, which is far away from the seventh transmission window 502, is provided with a third access window 505, the center of the third access window 505 is provided with two fourth operation holes 506 corresponding to the first waste liquid treatment barrel 503 and the second waste liquid treatment barrel 504, one side of the top of the front end of the waste treatment glass window 501 is provided with a fourth central control platform 507, and after detection is completed, the waste liquid can be collected and treated by the waste treatment device 5.
The reagent preparing glass cabinet 201, the nucleic acid extracting glass cabinet 301, the nucleic acid amplifying glass window 401 and the waste processing glass window 501 are all made of transparent toughened glass.
The first access panel 305, the second access panel 405, and the third access panel 505 are each configured in a rectangular configuration.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A nucleic acid detection combination module, includes unable adjustment base (1), its characterized in that: one side at unable adjustment base (1) top is provided with reagent and prepares device (2), one side that reagent prepared device (2) is provided with nucleic acid extraction device (3), one side that nucleic acid extraction device (3) kept away from reagent and prepared device (2) is provided with nucleic acid amplification device (4), one side that nucleic acid extraction device (3) were kept away from in nucleic acid amplification device (4) is provided with waste treatment device (5).
2. A combined nucleic acid detection module according to claim 1, wherein: the reagent preparing device (2) comprises a reagent preparing glass cabinet (201), two sides of the bottom of the front end of the reagent preparing glass cabinet (201) are respectively and rotatably connected with a first transmission window (202) and a second transmission window (203), an operation platform (204) is arranged in the center of the reagent preparation glass cabinet (201), a detection kit (205) is arranged at the top of the operation platform (204), the rear end of the center of the reagent preparing glass cabinet (201) is provided with an operating frame (206), a plurality of test tubes (207) are arranged at the top of the operating frame (206), pipettors (208) corresponding to the detection reagent boxes (205) are arranged at one side of the test tubes (207), two first operation holes (209) are arranged at the center of the front end of the reagent preparing glass cabinet (201), a first central control platform (210) is installed on one side of the top of the front end of the reagent preparation glass cabinet (201).
3. A combined nucleic acid detection module according to claim 1, wherein: nucleic acid extraction device (3) are including nucleic acid extraction glass cabinet (301), one side of reagent preparation device (2) is provided with nucleic acid extraction glass cabinet (301), the both sides of nucleic acid extraction glass cabinet (301) front end bottom are rotated respectively and are connected with third delivery window (302) and fourth delivery window (303), the center of nucleic acid extraction glass cabinet (301) is provided with full-automatic nucleic acid extraction appearance (304), the center of nucleic acid extraction glass cabinet (301) front end is provided with first access window (305) corresponding with full-automatic nucleic acid extraction appearance (304), two second handle holes (306) have been seted up at the center of first access window (305), control platform (307) in the second corresponding with full-automatic nucleic acid extraction appearance (304) is installed to one side at nucleic acid extraction glass cabinet (301) front end top.
4. A combined nucleic acid detection module according to claim 1, wherein: the nucleic acid amplification device (4) comprises a nucleic acid amplification glass window (401), one side, far away from the reagent preparation device (2), of the nucleic acid extraction device (3) is provided with the nucleic acid amplification glass window (401), two sides of the bottom of the front end of the nucleic acid amplification glass window (401) are respectively connected with a fifth transmission window (402) and a sixth transmission window (403) in a rotating mode, the center of the nucleic acid amplification glass window (401) is provided with a PCR instrument (404), the center of the front end of the nucleic acid amplification glass window (401) is provided with a second access window (405) corresponding to the PCR instrument (404), the center of the second access window (405) is provided with two third operation holes (406) corresponding to the PCR instrument (404), and one side of the top of the front end of the nucleic acid amplification glass window (401) is provided with a third central control platform (407).
5. A combined nucleic acid detection module according to claim 1, wherein: the waste treatment device (5) comprises a waste treatment glass window (501), the side of the nucleic acid amplification device (4) far away from the nucleic acid extraction device (3) is provided with the waste treatment glass window (501), a seventh transfer window (502) is rotatably connected to one side of the bottom of the front end of the waste treatment glass window (501), the center of the waste treatment glass window (501) is respectively provided with a first waste liquid treatment barrel (503) and a second waste liquid treatment barrel (504), a third access window (505) is arranged on one side of the front end of the waste treatment glass window (501) far away from the seventh transfer window (502), the center of the third access window (505) is provided with two fourth operation holes (506) corresponding to the first waste liquid treatment barrel (503) and the second waste liquid treatment barrel (504), and a fourth central control platform (507) is arranged on one side of the top of the front end of the waste treatment glass window (501).
6. A combined nucleic acid detection module according to any one of claims 2 to 5, in which: the reagent preparation glass cabinet (201), the nucleic acid extraction glass cabinet (301), the nucleic acid amplification glass window (401) and the waste treatment glass window (501) are all made of transparent toughened glass.
7. A combined nucleic acid detection module according to any one of claims 3 to 5, in which: the first access panel (305), the second access panel (405) and the third access panel (505) are all arranged in a rectangular structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010734240.XA CN111826281A (en) | 2020-07-28 | 2020-07-28 | Nucleic acid detects composite module |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010734240.XA CN111826281A (en) | 2020-07-28 | 2020-07-28 | Nucleic acid detects composite module |
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| CN111826281A true CN111826281A (en) | 2020-10-27 |
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| CN202010734240.XA Withdrawn CN111826281A (en) | 2020-07-28 | 2020-07-28 | Nucleic acid detects composite module |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022088421A1 (en) * | 2020-11-02 | 2022-05-05 | 深圳华大基因股份有限公司 | Quick-building-type test laboratory for medical test |
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2020
- 2020-07-28 CN CN202010734240.XA patent/CN111826281A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022088421A1 (en) * | 2020-11-02 | 2022-05-05 | 深圳华大基因股份有限公司 | Quick-building-type test laboratory for medical test |
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Application publication date: 20201027 |
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