CN111454839A - New coronavirus nucleic acid preparation and PCR detection integrated device - Google Patents
New coronavirus nucleic acid preparation and PCR detection integrated device Download PDFInfo
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 156
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 156
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 156
- 238000001514 detection method Methods 0.000 title claims abstract description 50
- 241000711573 Coronaviridae Species 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 238000000605 extraction Methods 0.000 claims abstract description 123
- 238000004458 analytical method Methods 0.000 claims abstract description 3
- 239000002699 waste material Substances 0.000 claims description 24
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 claims description 8
- 239000011324 bead Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 4
- 235000011613 Pinus brutia Nutrition 0.000 claims description 4
- 241000018646 Pinus brutia Species 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 3
- 238000007781 pre-processing Methods 0.000 claims 2
- 239000000284 extract Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 47
- 238000000034 method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 241000700605 Viruses Species 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 4
- 241001678559 COVID-19 virus Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
The application relates to a new coronavirus nucleic acid preparation and PCR detection integrated device, which comprises: a housing; a sample processing module for sucking a sample from the sample stage; a nucleic acid extraction module for extracting nucleic acid; a nucleic acid transfer module including a third conveyor belt and a nucleic acid extraction gun that extracts nucleic acids from the nucleic acid extraction plate; the PCR detection module comprises a PCR tube, a temperature control bottom plate, a laser emitter, a fluorescence signal detection device, a data processing and control element and a data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser emitter emits laser to irradiate the PCR tube, the fluorescence signal detection device detects the fluorescence signal in the PCR tube, and the data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.
Description
Technical Field
The invention relates to the technical field of molecular detection, in particular to a novel coronavirus nucleic acid preparation and PCR detection integrated device.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
The detection of nucleic acid of new coronavirus (severe acute respiratory syndrome coronavirus 2, 2019-nCoV, severe acute respiratory syndrome coronavirus 2) is carried out in a P3 laboratory due to extremely strong infectivity, an operator needs to wear protective clothing, a barrier coat, goggles and the like to enter a P3 laboratory for extraction, and the nucleic acid is spotted into a PCR system for real-time fluorescence quantitative PCR detection after extraction. It is extremely inconvenient and time-consuming to wear protective clothing and the like when extracting nucleic acid, and the operator is easy to have the condition of oxygen deficiency in the operation process, and is easy to have fatigue in long-term operation, thereby improving the probability of infecting virus. The real-time quantitative PCR requires a process of 2 hours, and the concentrated specimens are put on the machine together, so that scattered specimens cannot be made, and the method cannot be used for emergency treatment projects, which can prevent the detection of the new coronavirus from being carried out timely and conveniently.
Disclosure of Invention
In view of the above, there is a need to provide an integrated apparatus for preparing and detecting nucleic acid of new coronavirus, which integrates the functions of preparing and detecting nucleic acid of new coronavirus.
An integrated device for preparing and PCR detecting new coronavirus nucleic acid, comprising:
a housing;
the sample processing module comprises a sample suction gun, a first conveying belt and a sample table, and the sample table is connected to the first conveying belt to convey the sample table; the sample suction gun is movably connected with the shell and is positioned above the sample table and used for sucking a sample from the sample table;
the nucleic acid extraction module comprises a first telescopic rod, a second telescopic rod, an extraction magnetic frame, a nucleic acid extraction plate and a second conveyor belt, wherein the first telescopic rod is connected to the magnetic rod of the extraction magnetic frame so as to drive the magnetic rod to move along the vertical direction; the second telescopic rod is connected to the magnetic force extraction frame to drive the magnetic force extraction frame to move along the horizontal direction, the magnetic force extraction frame is provided with a through hole with a small upper part and a large lower part, the magnetic force extraction frame further comprises a suction head, the suction head is connected to the plastic sleeve, and the suction head is adsorbed at the bottom of the through hole through magnetic force; the nucleic acid extraction plate is connected to the second conveying belt, and the second conveying belt moves along the horizontal direction to drive the nucleic acid extraction plate to move along the horizontal direction;
a nucleic acid transfer module including a third conveyor belt and a nucleic acid extraction gun, the nucleic acid extraction gun being extended and retracted in a vertical direction to extract nucleic acids from the nucleic acid extraction plate, the third conveyor belt being connected to the nucleic acid extraction gun to move the nucleic acid extraction gun in a horizontal direction;
the PCR detection module comprises a PCR tube, a temperature control bottom plate, a laser emitter, a fluorescent signal detection device, a data processing and control element and a data output panel, wherein the PCR tube is arranged on the temperature control bottom plate, the laser emitter faces the PCR tube to irradiate laser to the PCR tube, the fluorescent signal detection device is positioned above the PCR tube, the fluorescent signal detection device is connected with the data processing and control element, and the data processing and control element is connected with the data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser emitter emits laser to irradiate the PCR tube, the fluorescence signal detection device detects the fluorescence signal in the PCR tube, and the data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.
Preferably, the housing includes a main processing chamber and a pretreatment chamber, the nucleic acid extraction module is located in the main processing chamber, and the first transport belt has one end located in the pretreatment chamber and the other end located in the main processing chamber and below the pipette.
Preferably, a PCR processing chamber is arranged in the shell, a normally closed door is arranged in the PCR processing chamber, the PCR detection module is arranged in the PCR processing chamber, and after nucleic acid is extracted by the nucleic acid extraction gun, the nucleic acid is added into the PCR tube through the normally closed door.
Preferably, a waste plate processing chamber is further arranged in the housing, one end of the third conveyor belt is located in the main processing chamber and below the extraction magnetic frame, and the other end of the third conveyor belt extends into the waste plate processing chamber to drive the nucleic acid extraction plate to enter the waste plate processing chamber.
Preferably, still include flexible platform and apron, flexible platform is flexible along vertical direction and connect in the apron, the apron is located the top of second transmission band, when the nucleic acid extraction board follows the second transmission band gets into abandonment board treatment chamber, flexible platform drives the apron lock is in order to seal the nucleic acid extraction board on the nucleic acid extraction board.
Preferably, one or more uv disinfection tubes are provided within the main chamber, waste plate chamber and PCR chamber.
Preferably, the nucleic acid extraction plate comprises a first extraction box, a second extraction box and a third extraction box, wherein magnetic beads are arranged in the first extraction box, washing liquid is arranged in the second extraction box, and eluent is arranged in the third extraction box.
Preferably, a gun head box is further arranged in the shell, and a gun head corresponding to the nucleic acid extraction gun is arranged in the gun head box.
Preferably, a first collector is arranged in the pretreatment chamber, and a pine hypochlorite solution is arranged in the first collector and is used for collecting the waste sample table.
Preferably, the waste plate treatment chamber is internally provided with a second collector, and the second collector is internally provided with a pine hypochlorite solution for collecting the waste nucleic acid extraction plate.
Compared with the prior art, the new coronavirus nucleic acid preparation and PCR detection integrated device can integrate nucleic acid extraction and real-time fluorescence quantitative PCR, after the whole sample is placed on the sample stage, the sample suction gun of the sample processing module adds the sample into the nucleic acid extraction plate of the nucleic acid extraction module, the nucleic acid with high purity is extracted after the vibration of the extraction magnetic frame, then the nucleic acid transfer module transfers the extracted nucleic acid to the PCR tube of the PCR detection module which is placed in advance, fluorescence is generated through the excitation of the laser emitter, a fluorescence signal is received by the fluorescence signal detection device, is analyzed by the data processing and control element, and finally is output to the data output panel for display.
The integrated device for preparing the new coronavirus nucleic acid and detecting the PCR greatly shortens the whole nucleic acid extraction and PCR process, and the shell which is closed and can be disinfected by ultraviolet is adopted to effectively treat and store the treated waste, so that the safety can be effectively ensured. Because the amplification enzyme can be amplified only at constant temperature, the samples can be extracted at any time and PCR amplification can be carried out at any time, so that the operation can be carried out all weather, no appointment time is needed, scattered samples can be prepared, and the emergency call requirement can be met. The probe system aiming at the new corona nucleic acid in the PCR tube is changed into the probe aiming at other viruses or bacteria, or different probes are arranged in different tubes, the same sample nucleic acid can realize the simultaneous detection of a plurality of viruses, and the method has wide application and wide market prospect. The novel integrated device for preparing the coronavirus nucleic acid and detecting the PCR can be used in hospital clinical laboratory and various public places needing to be detected.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic diagram of the appearance structure of the integrated device for preparing and PCR detecting new coronavirus nucleic acid.
FIG. 2 is a schematic diagram of the internal structure of the integrated device for preparing and PCR detecting new coronavirus nucleic acid.
FIG. 3 is a schematic structural diagram of an extraction magnetic frame of the integrated device for preparing nucleic acid of the novel coronavirus and detecting PCR.
Description of the main elements
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
Detailed Description
So that the manner in which the above recited objects, features and advantages of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may be had by reference to the embodiments thereof which are illustrated in the appended drawings. In addition, the embodiments and features of the embodiments of the present application may be combined with each other without conflict. In the following description, numerous specific details are set forth to provide a thorough understanding of the present invention, and the described embodiments are merely a subset of the embodiments of the present invention, rather than a complete embodiment. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
In various embodiments of the present invention, for convenience in description and not in limitation, the term "coupled" as used in the specification and claims of the present application is not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships are changed accordingly.
FIG. 1 is a schematic diagram of the appearance structure of the integrated device for preparing nucleic acid of new coronavirus and PCR detection, and FIG. 2 is a schematic diagram of the internal structure of the integrated device for preparing nucleic acid of new coronavirus and PCR detection. As shown in FIGS. 1 and 2, the integrated device for preparing and PCR detecting new coronavirus nucleic acid comprises a housing 10, a sample processing module, a nucleic acid extraction module, a nucleic acid transfer module and a PCR detection module. In use, a sample is pre-treated by the sample processing module and input into the housing 10. Then, the nucleic acid of the sample is extracted through the nucleic acid extraction module, and is transferred into the PCR detection module through the nucleic acid transfer module for detection, so that the functions of nucleic acid preparation and PCR detection of the new coronavirus are realized.
The casing 10 is hollow inside, and has a first window 111 and a second window 141 on the outer wall, and a pretreatment chamber 11, a main treatment chamber 12, a PCR treatment chamber 13, and a waste plate treatment chamber 14 inside. One or more uv disinfection tubes 15 are provided in the main chamber 12, waste plate chamber 14 and PCR chamber 13. The chambers are normally isolated from each other by isolation doors. When the interaction objects are needed, the isolation door (which can be an automatic door) is opened to realize interconnection among the chambers.
The sample processing module includes a suction gun 22, a first conveyor belt 20 and a sample stage 21. The sample stage 21 is connected to the first conveyer belt 20 to convey the sample stage 21, and the first conveyer belt 20 has one end located in the pretreatment chamber 11 and the other end located in the main treatment chamber 12 and below the pipette 22. The suction gun 22 is disposed in the main processing chamber 12 and movably connected to the housing 10 above the sample stage 21 for sucking a sample from the sample stage 21. In addition, a first collector 112 is arranged in the pretreatment chamber 11, and a loose hypochlorous acid liquid is arranged in the first collector 112 and is used for collecting the waste sample table 21.
Fig. 3 is a schematic structural diagram of an extraction magnetic rack of the integrated device for preparing nucleic acid of new coronavirus and detecting PCR, as shown in fig. 2 and 3, a nucleic acid extraction module is located in the main processing chamber 12, and comprises a first telescopic rod 31, a second telescopic rod 32, an extraction magnetic rack 33, a nucleic acid extraction plate 34 and a second conveyor belt 30. The first telescopic rod 31 is connected to the magnetic rod 311 of the magnetic force extraction frame 33 to drive the magnetic rod 311 to move in the vertical direction. In this embodiment, the first telescopic rod 31 may be connected to the plurality of magnetic rods 311 through plate bodies to simultaneously operate the plurality of sets of nucleic acid extracting plates 34. The second telescopic rod 32 is connected to the magnetic force extracting rack 33 to drive the magnetic force extracting rack 33 to move along the horizontal direction. The magnetic force extraction rack 33 is provided with a plurality of through holes 332 with small cross sections and large cross sections, and the length direction of the through holes 332 is arranged along the vertical direction. The magnetic force extraction rack 33 further comprises a suction head 333, the suction head 333 is sleeved at the end of the plastic sleeve 331, and the suction head 333 is adsorbed at the bottom of the through hole 332 through magnetic force. In this way, the extraction magnetic rack 33 is connected to the plastic sleeve 331 through the suction head 333, wherein the plastic sleeve 331 is replaceable.
The nucleic acid extracting plates 34 are connected to the second conveyor belt 30. in this embodiment, a plurality of sets of nucleic acid extracting plates 34 may be disposed on the second conveyor belt 30 in a direction perpendicular to the paper surface, so that nucleic acid extracting operations can be simultaneously performed on a plurality of nucleic acid extracting plates 34. Corresponding to the magnetic bars 311, the nucleic acid extracting plates 34 may also be provided with a plurality of sets of nucleic acid extracting plates 34 along the length direction of the second conveyor belt 30 to perform the nucleic acid extracting work simultaneously. The nucleic acid extraction plate 34 comprises a first extraction cassette, 3 second extraction cassettes and a third extraction cassette. The first extraction box is internally provided with magnetic beads, the 3 second extraction boxes are respectively provided with washing liquid, and the third extraction box is provided with eluent. The second conveyor belt 30 is moved in the horizontal direction to move the nucleic acid extracting plate 34 in the horizontal direction. Wherein, new coronavirus nucleic acid preparation and PCR detection integrated device still includes flexible platform and apron, flexible platform is flexible along vertical direction and connect in the apron, the apron is located the top of second transmission band 30 nucleic acid extraction board 34 follows second transmission band 30 gets into when abandonment board treatment chamber 14, flexible platform drives the apron lock is in order to seal on the nucleic acid extraction board 34. The waste plate treatment chamber 14 is provided with a second collector, in which a loose hypochlorous acid solution is provided for collecting the waste nucleic acid extraction plate 34.
The nucleic acid transfer module includes a third conveyor belt 40 and a nucleic acid extraction gun 41, and the nucleic acid extraction gun 41 is extended and contracted in the vertical direction to extract nucleic acid from the nucleic acid extraction plate 34. The third conveyor 40 is connected to the nucleic acid extraction gun 41 to move the nucleic acid extraction gun 41 in a horizontal direction. The third conveyor belt 40 has one end located in the main processing chamber 12 and below the extraction magnetic rack 33, and the other end extending into the waste plate processing chamber 14 to carry the nucleic acid extraction plate 34 into the waste plate processing chamber 14. One or more gun head boxes 16 are also arranged in the main treatment chamber 12, and the nucleic acid extraction gun 41 can automatically replace the gun heads from the gun head boxes 16 when needed.
The PCR detection module comprises a PCR tube 50, a temperature control bottom plate 51, a laser emitter 52, a fluorescence signal detection device 53, a data processing and control element 54 and a data output panel 55. The PCR detection module is arranged in the PCR processing chamber 13, the PCR processing chamber 13 is provided with a normally closed door, and after nucleic acid is extracted by the nucleic acid extraction gun 41, the nucleic acid is added into the PCR tube 50 through the normally closed door. The PCR tube 50 is disposed on the temperature-controlled bottom plate 51, the laser emitter 52 faces the PCR tube 50 to irradiate laser to the PCR tube 50, the fluorescence signal detection device 53 is disposed above the PCR tube 50, the fluorescence signal detection device 53 is connected to the data processing control element, and the data processing control element is connected to the data output panel 55.
The working process of the integrated device for preparing the nucleic acid of the novel coronavirus and detecting the PCR is described in detail below.
When in use, the whole specimen is placed on the sample table 21 for ultraviolet disinfection, and then is conveyed to the lower part of the sample suction gun 22 by the first conveying belt 20; the sample suction gun 22 sucks a sample from the sample table 21 and adds the sample into a nucleic acid extraction plate 34 which is added with magnetic beads and washing solution in advance;
then, the first telescopic rod 31 drives the magnetic rod 311 to move downwards until the magnetic rod extends into the plastic sleeve 331, so that the suction head 333 is separated from the magnetic force extraction rack 33 and is adsorbed on the magnetic rod 311, and at this time, the plastic sleeve 331 is sleeved on the bottom of the magnetic rod 311.
Then, the first telescopic rod 31 continues to drive the magnetic rod 311 to move downwards and extend into the first hole of the nucleic acid extraction plate 34 to extract the magnetic beads in the first hole of the nucleic acid extraction plate 34, and then moves upwards until the magnetic rod 311 is separated from the nucleic acid extraction plate 34. At this time, the plastic sleeve 331 is still located below the magnetic force extraction rack 33, and the suction head 333 is adsorbed on the magnetic force bar 331, and the plastic sleeve 331 is sleeved on the bottom of the magnetic force bar 331.
Then, the second transfer belt 30 is moved so that the second well of the nucleic acid extracting plate 34 is moved to below the magnetic bar 311; the first telescopic rod 31 continues to move downwards until the magnetic beads adsorbed on the plastic sleeve 331 enter the second hole of the nucleic acid extracting plate 34 for washing. Then, the washing is continued in the same manner in the third well, and then, the washing is continued in the fourth well.
Then, the first telescopic rod 31 drives the magnetic rod 311 to move upwards until the suction head 333 abuts against the magnetic force extraction rack 33. Since the through hole 332 is large up and down, the attraction head 333 is blocked by the extraction magnetic frame 33 and attracted to the extraction magnetic frame 33. As the magnetic rod 311 continues to move upward, the tip 333 and the plastic sleeve 331 are allowed to disengage from the magnetic rod 311, and the magnetic beads fall into the fourth well of the nucleic acid extraction plate 34 due to the loss of magnetic force.
Finally, the magnetic rod 311 is moved to the upper side of the extraction magnetic rack 33, and the next nucleic acid extraction operation is prepared. When necessary, the second telescopic rod 32 is retracted to replace the suction head 333 and the plastic sleeve 331 on the extraction magnetic frame 33. Through this step, the nucleic acid with high purity is eluted, and the whole extraction process is about 20 minutes.
Then, the second conveyor belt 30 drives the nucleic acid extracting plate 34 to move to a proper position, the nucleic acid extracting gun 41 moved by the third conveyor belt 40 sucks nucleic acid from the nucleic acid extracting plate 34, the nucleic acid is added into a PCR tube 50 (containing a prepared PCR system) which is placed in advance, a temperature control bottom plate 51 is arranged below the PCR tube 50, and enzyme for constant temperature amplification is arranged in the PCR system, so that DNA amplification can be carried out at 65 ℃ without denaturation annealing extension. The DNA amplification is started as soon as the DNA is added, the standing time is set for 1 hour for each sample, fluorescence is generated through the excitation of the laser emitter 52, a fluorescence signal is received by the fluorescence signal detection device 53, and the fluorescence signal is analyzed through the data processing and control element 54 and finally output to the data output panel 55 for displaying.
The above-mentioned new coronavirus nucleic acid preparation and PCR detection integrated device can integrate nucleic acid extraction and real-time fluorescence quantitative PCR, after the whole sample is placed in the sample stage 21, the sample suction gun 22 of the sample processing module adds the sample into the nucleic acid extraction plate 34 of the nucleic acid extraction module, and the nucleic acid with high purity is extracted after the vibration of the extraction magnetic frame 33, and then the nucleic acid transfer module transfers the extracted nucleic acid to the PCR tube 50 of the PCR detection module placed in advance, and fluorescence is generated by the excitation of the laser emitter 52, and the fluorescence signal is received by the fluorescence signal detection device 53, and is analyzed by the data processing and control element 54, and finally output to the data output panel 55 for display.
The integrated device for preparing the new coronavirus nucleic acid and detecting the PCR greatly shortens the whole nucleic acid extraction and PCR process, and the shell which is closed and can be disinfected by ultraviolet is adopted to effectively treat and store the treated waste, so that the safety can be effectively ensured. Because the amplification enzyme can be amplified only at constant temperature, the samples can be extracted at any time and PCR amplification can be carried out at any time, so that the operation can be carried out all weather, no appointment time is needed, scattered samples can be prepared, and the emergency call requirement can be met. The probe system aiming at the new corona nucleic acid in the PCR tube 50 is changed into the probe aiming at other viruses or bacteria, or different probes are arranged in different tubes, the same sample nucleic acid can realize the simultaneous detection of a plurality of viruses, and the method has wide application and wide market prospect. The novel integrated device for preparing the coronavirus nucleic acid and detecting the PCR can be used in hospital clinical laboratory and various public places needing to be detected.
In the several embodiments provided in the present invention, it should be understood that it is apparent to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, but can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned. Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. The terms first, second, etc. are used to denote names, but not any particular order.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention.
Claims (10)
1. An integrated device for preparing and PCR detecting new coronavirus nucleic acid is characterized by comprising:
a housing;
the sample processing module comprises a sample suction gun, a first conveying belt and a sample table, and the sample table is connected to the first conveying belt to convey the sample table; the sample suction gun is movably connected with the shell and is positioned above the sample table and used for sucking a sample from the sample table;
the nucleic acid extraction module comprises a first telescopic rod, a second telescopic rod, an extraction magnetic frame, a nucleic acid extraction plate and a second conveyor belt, wherein the first telescopic rod is connected to the magnetic rod of the extraction magnetic frame so as to drive the magnetic rod to move along the vertical direction; the second telescopic rod is connected to the magnetic force extraction frame to drive the magnetic force extraction frame to move along the horizontal direction, the magnetic force extraction frame is provided with a through hole with a small upper part and a large lower part, the magnetic force extraction frame further comprises a suction head, the suction head is connected to the plastic sleeve, and the suction head is adsorbed at the bottom of the through hole through magnetic force; the nucleic acid extraction plate is connected to the second conveying belt, and the second conveying belt moves along the horizontal direction to drive the nucleic acid extraction plate to move along the horizontal direction;
a nucleic acid transfer module including a third conveyor belt and a nucleic acid extraction gun, the nucleic acid extraction gun being extended and retracted in a vertical direction to extract nucleic acids from the nucleic acid extraction plate, the third conveyor belt being connected to the nucleic acid extraction gun to move the nucleic acid extraction gun in a horizontal direction;
the PCR detection module comprises a PCR tube, a temperature control bottom plate, a laser emitter, a fluorescent signal detection device, a data processing and control element and a data output panel, wherein the PCR tube is arranged on the temperature control bottom plate, the laser emitter faces the PCR tube to irradiate laser to the PCR tube, the fluorescent signal detection device is positioned above the PCR tube, the fluorescent signal detection device is connected with the data processing and control element, and the data processing and control element is connected with the data output panel; after the nucleic acid extraction gun adds the extracted nucleic acid into the PCR tube, the laser emitter emits laser to irradiate the PCR tube, the fluorescence signal detection device detects the fluorescence signal in the PCR tube, and the data processing and control element receives the detection signal of the fluorescence signal detection device and displays the analysis result on the data output panel.
2. The integrated apparatus for preparing and PCR detecting a new coronavirus nucleic acid of claim 1, wherein the housing comprises a main processing chamber and a pre-processing chamber, the nucleic acid extraction module is located in the main processing chamber, and the first conveyor belt has one end located in the pre-processing chamber and the other end located in the main processing chamber and located under the pipette.
3. The integrated apparatus for preparing and detecting nucleic acid of new coronavirus according to claim 2, wherein the casing is provided with a PCR processing chamber, the PCR processing chamber is provided with a normally closed door, the PCR detection module is arranged in the PCR processing chamber, and after nucleic acid is extracted by the nucleic acid extraction gun, the nucleic acid is added into the PCR tube through the normally closed door.
4. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR of claim 3, wherein a waste plate processing chamber is further disposed in the housing, one end of the third conveyor belt is disposed in the main processing chamber and below the magnetic rack, and the other end of the third conveyor belt extends into the waste plate processing chamber to drive the nucleic acid extraction plate into the waste plate processing chamber.
5. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR as claimed in claim 4, further comprising a retractable stage and a cover plate, wherein the retractable stage is retractable in a vertical direction and connected to the cover plate, the cover plate is located above the second conveyor belt, and when the nucleic acid extraction plate follows the second conveyor belt and enters the waste plate treatment chamber, the retractable stage drives the cover plate to be fastened on the nucleic acid extraction plate so as to seal the nucleic acid extraction plate.
6. The integrated apparatus for preparing and PCR detecting the nucleic acid of the new coronavirus according to claim 5, wherein one or more UV disinfection tubes are disposed in the main processing chamber, the waste plate processing chamber and the PCR processing chamber.
7. The integrated apparatus for preparing nucleic acid of novel coronavirus and detecting PCR of claim 6, wherein the nucleic acid extraction plate comprises a first extraction cartridge, a second extraction cartridge and a third extraction cartridge, wherein the first extraction cartridge is provided with magnetic beads, the second extraction cartridge is provided with a washing solution, and the third extraction cartridge is provided with an eluent.
8. The integrated apparatus for preparing nucleic acid of new coronavirus and detecting PCR according to claim 7, wherein a gun head box is further arranged in the casing, and a gun head corresponding to the nucleic acid extraction gun is arranged in the gun head box.
9. The integrated apparatus for preparing and PCR detecting new coronavirus nucleic acid of claim 8, wherein a first collector is arranged in the pretreatment chamber, and a pine hypochlorite solution is arranged in the first collector and is used for collecting a waste sample table.
10. The integrated apparatus for preparing nucleic acid from a novel coronavirus and detecting PCR of claim 9, wherein the waste plate treatment chamber is provided with a second collector, and the second collector is provided with a pine hypochlorite solution for collecting the waste nucleic acid extraction plate.
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