CN112522084A - Sampling and PCR detection integrated device for detecting new coronavirus and use method - Google Patents
Sampling and PCR detection integrated device for detecting new coronavirus and use method Download PDFInfo
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- CN112522084A CN112522084A CN202011321914.XA CN202011321914A CN112522084A CN 112522084 A CN112522084 A CN 112522084A CN 202011321914 A CN202011321914 A CN 202011321914A CN 112522084 A CN112522084 A CN 112522084A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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Abstract
The invention provides a sampling and PCR detection integrated device for detecting new coronavirus, which comprises a sampling cup, a sampling bag, a sampling tube and a sampling flat-laying chamber, wherein the sampling flat-laying chamber is provided with a passage for the sampling tube to pass through, the sampling cup is screwed with the lower end of the sampling flat-laying chamber through threads, the sampling tube is positioned in the sampling cup, and the upper end of the sampling tube is provided with the sampling bag. The invention has the beneficial effects that: the invention can detect more samples and improve the detection efficiency and accuracy.
Description
Technical Field
The invention relates to the field of virus detection, in particular to a sampling and PCR detection integrated device for detecting new coronavirus and a using method thereof.
Background
The PCR detection method belongs to a new gene diagnosis technology, requires sterile operation, and is put into a test tube with the caliber of 1.0 cm for assay so as to improve the accuracy. At present, the bacteria are easy to carry during sampling in hospitals, samples are often polluted or polluted, aseptic operation sampling cannot be achieved, and conveying is inconvenient.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides a sampling and PCR detection integrated device for detecting new coronavirus and a using method thereof.
The purpose of the invention is realized by the following technical scheme.
New corona virus detects with sample and PCR detection integrated device, including sampling cup, sample bag, sampling tube and sample tiling room constitution, be equipped with on the sample tiling room and supply the passageway that the sampling tube passed, the sampling cup with the lower extreme of sample tiling room is screwed up through the screw thread, the sampling tube is located in the sampling cup, the upper end of sampling tube is equipped with the sample bag.
Preferably, the sampling capsule is made of rubber or rubber.
Preferably, in any of the above embodiments, the sampling cup and the sampling tube are made of plastic or glass.
Preferably by any one of the above schemes, the sample tiling chamber includes sample introduction pipeline, tiling chamber and cleaner pipeline, sample introduction pipeline's bottom extends into in the sample cup, sample introduction pipeline's top with the tiling chamber is linked together, the cleaner pipeline with the tiling chamber is linked together.
A method for using a sampling and PCR detection integrated device for detecting new coronavirus comprises the following steps of firstly, connecting a sample injection device containing a cleaning agent to a cleaning agent pipeline;
injecting a cleaning agent into the flat-laying chamber through a cleaning agent pipeline by using a sample injection device so as to fill the flat-laying chamber with the cleaning agent;
injecting a sample into the tiling chamber through an upper sample injection pipeline by using sample injection equipment, wherein the sample forms single-layer tiling in the tiling chamber;
step four, detecting the samples in the tiled chamber through external detection equipment;
and step five, after the detection is finished, injecting a cleaning agent into the flatting chamber through a cleaning agent pipeline through a sample injection device, and flushing out original liquid drops and other substances in the cleaning agent pipeline and the flatting chamber due to the injection of the cleaning agent to finish the detection.
Preferably, in the third step, the specific steps of obtaining the sample include:
the first step, a sampling cup is disinfected and screwed below a sampling tiling chamber to be in a sealed state;
in the second step, the sampling tube injects the sample into the sampling cup.
The invention has the beneficial effects that:
after being sterilized, the sampling cup is sealed, so that the pollution can be prevented, the transportation is convenient, and the sampling is convenient.
The invention can detect more samples and improve the detection efficiency and accuracy.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
in the figure:
1. a sampling cup; 2. a sampling tube; 3. a tiling chamber; 4. a sample introduction pipeline; 5. a cleaning agent pipeline;
6. a sampling capsule; 7. and a sampling tube.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
As shown in fig. 1 and 2, 1 is a sampling cup; 2 is a sampling tube; 3 is a flat laying chamber; 4 is a sample introduction pipeline; 5 is a cleaning agent pipeline; 6 is a sampling capsule; and 7 is a sampling tube.
Example 1
New corona virus detects with sample and PCR detection integrated device, including sampling cup, sample bag, sampling tube and sample tiling room constitution, be equipped with on the sample tiling room and supply the passageway that the sampling tube passed, the sampling cup with the lower extreme of sample tiling room is screwed up through the screw thread, the sampling tube is located in the sampling cup, the upper end of sampling tube is equipped with the sample bag.
Example 2
New corona virus detects with sample and PCR detection integrated device, including sampling cup, sample bag, sampling tube and sample tiling room constitution, be equipped with on the sample tiling room and supply the passageway that the sampling tube passed, the sampling cup with the lower extreme of sample tiling room is screwed up through the screw thread, the sampling tube is located in the sampling cup, the upper end of sampling tube is equipped with the sample bag.
Preferably, the sampling capsule is made of rubber or rubber; the sampling cup and the sampling tube are both made of plastic or glass.
Example 3
New corona virus detects with sample and PCR detection integrated device, including sampling cup, sample bag, sampling tube and sample tiling room constitution, be equipped with on the sample tiling room and supply the passageway that the sampling tube passed, the sampling cup with the lower extreme of sample tiling room is screwed up through the screw thread, the sampling tube is located in the sampling cup, the upper end of sampling tube is equipped with the sample bag.
Preferably, the sampling capsule is made of rubber or rubber; the sampling cup and the sampling tube are both made of plastic or glass.
The sample tiling chamber includes sampling pipeline, tiling chamber and cleaner pipeline, sampling pipeline's bottom extends into in the sample cup, sampling pipeline's top with the tiling chamber is linked together, the cleaner pipeline with the tiling chamber is linked together.
A method for using a sampling and PCR detection integrated device for detecting new coronavirus comprises the following steps of firstly, connecting a sample injection device containing a cleaning agent to a cleaning agent pipeline;
injecting a cleaning agent into the flat-laying chamber through a cleaning agent pipeline by using a sample injection device so as to fill the flat-laying chamber with the cleaning agent;
injecting a sample into the tiling chamber through an upper sample injection pipeline by using sample injection equipment, wherein the sample forms single-layer tiling in the tiling chamber;
step four, detecting the samples in the tiled chamber through external detection equipment;
and step five, after the detection is finished, injecting a cleaning agent into the flatting chamber through a cleaning agent pipeline through a sample injection device, and flushing out original liquid drops and other substances in the cleaning agent pipeline and the flatting chamber due to the injection of the cleaning agent to finish the detection.
Preferably, in the third step, the specific steps of obtaining the sample include:
the first step, a sampling cup is disinfected and screwed below a sampling tiling chamber to be in a sealed state;
in the second step, the sampling tube injects the sample into the sampling cup.
The three embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (6)
1. New coronavirus detects with sample and PCR detection integrated device, its characterized in that: the sampling device comprises a sampling cup, a sampling bag, a sampling tube and a sampling flat-laying chamber, wherein a channel for the sampling tube to pass through is arranged on the sampling flat-laying chamber, the sampling cup is screwed with the lower end of the sampling flat-laying chamber through threads, the sampling tube is positioned in the sampling cup, and the sampling bag is arranged at the upper end of the sampling tube.
2. The integrated sampling and PCR detection device for detecting a new coronavirus according to claim 1, wherein: the sampling capsule is made of rubber or rubber.
3. The integrated sampling and PCR detection device for detecting a new coronavirus according to claim 2, wherein: the sampling cup and the sampling tube are both made of plastic or glass.
4. The integrated sampling and PCR detection device for detecting a new coronavirus according to claim 3, wherein: the sample tiling chamber includes sampling pipeline, tiling chamber and cleaner pipeline, sampling pipeline's bottom extends into in the sample cup, sampling pipeline's top with the tiling chamber is linked together, the cleaner pipeline with the tiling chamber is linked together.
5. A method for using the integrated sampling and PCR detection device for detecting a novel coronavirus according to any one of claims 1 to 4, wherein:
connecting a sample introduction device containing a cleaning agent to a cleaning agent pipeline;
injecting a cleaning agent into the flat-laying chamber through a cleaning agent pipeline by using a sample injection device so as to fill the flat-laying chamber with the cleaning agent;
injecting a sample into the tiling chamber through an upper sample injection pipeline by using sample injection equipment, wherein the sample forms single-layer tiling in the tiling chamber;
step four, detecting the samples in the tiled chamber through external detection equipment;
and step five, after the detection is finished, injecting a cleaning agent into the flatting chamber through a cleaning agent pipeline through a sample injection device, and flushing out original liquid drops and other substances in the cleaning agent pipeline and the flatting chamber due to the injection of the cleaning agent to finish the detection.
6. The use method of the integrated PCR detection device according to claim 5, wherein: in the third step, the specific steps of obtaining the sample comprise:
the first step, a sampling cup is disinfected and screwed below a sampling tiling chamber to be in a sealed state;
in the second step, the sampling tube injects the sample into the sampling cup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011321914.XA CN112522084A (en) | 2020-11-23 | 2020-11-23 | Sampling and PCR detection integrated device for detecting new coronavirus and use method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011321914.XA CN112522084A (en) | 2020-11-23 | 2020-11-23 | Sampling and PCR detection integrated device for detecting new coronavirus and use method |
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| CN202011321914.XA Pending CN112522084A (en) | 2020-11-23 | 2020-11-23 | Sampling and PCR detection integrated device for detecting new coronavirus and use method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2252987Y (en) * | 1995-09-24 | 1997-04-23 | 翁炳焕 | PCR detection method sampler |
| JP2006223125A (en) * | 2005-02-15 | 2006-08-31 | Shimadzu Corp | Reaction vessel, method and apparatus for detecting genetic polymorphism and method and apparatus for diagnosis |
| CN109337793A (en) * | 2018-10-23 | 2019-02-15 | 宁波艾捷康宁生物科技有限公司 | A kind of full-automatic nucleic acid extraction detection system |
| CN110358676A (en) * | 2019-08-17 | 2019-10-22 | 清华大学 | The microlayer model sampler applied in the detection of digital pcr imaging method |
| CN111454839A (en) * | 2020-05-11 | 2020-07-28 | 蔡祥胜 | New coronavirus nucleic acid preparation and PCR detection integrated device |
-
2020
- 2020-11-23 CN CN202011321914.XA patent/CN112522084A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2252987Y (en) * | 1995-09-24 | 1997-04-23 | 翁炳焕 | PCR detection method sampler |
| JP2006223125A (en) * | 2005-02-15 | 2006-08-31 | Shimadzu Corp | Reaction vessel, method and apparatus for detecting genetic polymorphism and method and apparatus for diagnosis |
| CN109337793A (en) * | 2018-10-23 | 2019-02-15 | 宁波艾捷康宁生物科技有限公司 | A kind of full-automatic nucleic acid extraction detection system |
| CN110358676A (en) * | 2019-08-17 | 2019-10-22 | 清华大学 | The microlayer model sampler applied in the detection of digital pcr imaging method |
| CN111454839A (en) * | 2020-05-11 | 2020-07-28 | 蔡祥胜 | New coronavirus nucleic acid preparation and PCR detection integrated device |
Non-Patent Citations (1)
| Title |
|---|
| 陈启宇等: "《"健康+"语录：活过120岁》", 31 August 2020, 上海科学技术出版社 * |
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Application publication date: 20210319 |
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