CN216550407U

CN216550407U - Anti-pollution nucleic acid check out test set

Info

Publication number: CN216550407U
Application number: CN202122766903.9U
Authority: CN
Inventors: 金霞; 胡彬
Original assignee: Sanji Biotechnology Zhejiang Co ltd
Current assignee: Sanji Biotechnology Zhejiang Co ltd
Priority date: 2021-11-10
Filing date: 2021-11-10
Publication date: 2022-05-17
Anticipated expiration: 2031-11-10

Abstract

The utility model relates to an anti-pollution nucleic acid detection device, which is applied to pet hospitals. The device comprises a box body structure for detection, wherein an airflow filtering device is arranged at the upper part in the box body structure, a nucleic acid detection device is arranged at the lower part in the box body structure, and an operation door is arranged on the front surface of the box body structure. The PCR reaction system is completely operated in a clean grade 100 environment by integrating the PCR function, the centrifugal function and the airflow filtering function into one device, so that the possibility of sample pollution is basically avoided.

Description

Anti-pollution nucleic acid check out test set

Technical Field

The utility model relates to an anti-pollution nucleic acid detection device, which is applied to pet hospitals.

Background

At present, the pet hospital has poor facility conditions and no special PCR laboratory, and when nucleic acid detection is carried out, operations such as sample treatment, reaction system configuration and the like are carried out under the condition of no anti-pollution measures, and then the samples are put into a PCR instrument for amplification detection. Even if the superclean bench is widely applied in the field of human medicine, the superclean bench cannot be placed in a test laboratory of a pet hospital due to large volume.

The defects of the prior art are as follows:

(1) the laboratory has no anti-pollution facility aiming at nucleic acid detection, so that the accuracy of nucleic acid detection is greatly reduced;

(2) even if a small number of pet hospitals with larger scales are provided with the clean bench, because the instruments used in nucleic acid detection and the clean bench are not integrally designed, articles are taken in and taken out frequently, and the pollution probability is greatly increased;

(3) the PCR equipment and the centrifugal equipment are in an open laboratory environment, and cannot be sterilized in time after each use, so that the next batch of detection samples are easily polluted.

SUMMERY OF THE UTILITY MODEL

The present invention aims to overcome the above-mentioned drawbacks of the prior art, and thus to provide an integrated design of contamination-preventive nucleic acid detecting apparatus, which replaces all operations in nucleic acid detection that may be exposed to a contaminated environment.

The technical scheme adopted by the utility model is as follows:

the utility model provides an anti-pollution nucleic acid check out test set, is including detecting with the box structure, upper portion in the box structure is equipped with the air current filter equipment, and the lower part in the box structure disposes nucleic acid detection device, and the box structure openly is equipped with the operating door.

Preferably, the airflow filtering device comprises a primary filter, a centrifugal fan and a high-efficiency filter which are sequentially arranged from top to bottom to form a vertical airflow duct. The air filtering system with vertical airflow design is introduced, so that the safety and the reliability of detection operation are greatly enhanced.

Preferably, the nucleic acid detecting apparatus includes a pipette, a PCR device, and a centrifuge, which are disposed in the casing structure. Integrates the three devices, improves the specificity of instruments, reduces the times of taking in and out, and reduces the pollution probability.

Preferably, an ultraviolet sterilizing lamp is arranged above the nucleic acid detecting device. And an ultraviolet sterilizing lamp is configured, and sterilization operation is performed in time after detection is completed every time, so that cross contamination among sample batches is avoided.

Preferably, the middle part of the box body structure is provided with a visible window.

Preferably, a pipettor support and a garbage can are further arranged at the lower part in the box body structure. Set up special garbage bin, in time retrieve used consumptive material, prevent that the consumptive material from becoming new pollution sources.

Preferably, the operation door is rotatably connected with the box body structure through a hinge, and the operation door is turned up from bottom to top and is attracted with the box body structure through a magnet.

Preferably, the front surface of the box body structure is also provided with a control panel, and the control panel is connected with the airflow filtering device and the nucleic acid detection device through data lines in a control mode.

Preferably, the lower part in the box body structure is a working area.

Preferably, the height of the box body structure is 50-70 cm.

Compared with the prior art, the product solves the following problems:

1. by integrating the PCR function, the centrifugal function and the airflow filtering function into one device, the operations of PCR reaction system configuration, centrifugation and the like are completely operated in a clean grade 100 environment, and the possibility of sample pollution is basically eliminated.

2. The equipment is provided with an ultraviolet sterilization system, and after each detection is finished, the ultraviolet sterilization can avoid the pollution among detection samples of different batches to the greatest extent.

3. The whole instrument is small in size, can be placed on a pet hospital experiment table, and is suitable for being configured in a pet hospital.

Drawings

In order to illustrate more clearly the embodiments of the utility model or solutions in the prior art, reference will now be made briefly to the attached drawings used in the description of the embodiments or solutions in the prior art, it being apparent that the drawings in the following description are some embodiments of the utility model and that, to a person skilled in the art, other drawings can be derived from them without inventive effort.

Fig. 1 is a schematic structural diagram of the product.

Fig. 2 is a perspective view of fig. 1.

Fig. 3 is a left side view of fig. 2.

Fig. 4 is a perspective view of fig. 1.

Description of reference numerals: the device comprises a box body structure 1, an airflow filtering device 2, a primary filter 21, a centrifugal fan 22, a high-efficiency filter 23, a nucleic acid detection device 3, a liquid transfer device 31, PCR equipment 32, a centrifugal machine 33, an operation door 4, an ultraviolet sterilizing lamp 5, a visible window 6, a liquid transfer device support 7, a garbage can 8, a hinge 9, a magnet 10, a bottom corner 11, a control panel 12 and a working area A.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The basic concept is as follows: an integrated nucleic acid detection instrument is designed to replace all operations possibly exposed to polluted environment in nucleic acid detection.

Example (b):

as shown in FIGS. 1 to 4, the present product is an antipollution nucleic acid detecting apparatus, which is a box structure 1 having a height of 50 to 70cm and a height of about 60cm, and is divided into an upper part and a lower part, wherein an airflow filter device 2 including a primary filter 21 (a filter for filtering dust particles of 5 μm or more), a centrifugal fan 22 and a high efficiency filter 23 (a filter for trapping particulate dust and various suspended substances of 0.5 μm or less) is disposed at the upper part, a hollow working area A is disposed at the lower part, and a nucleic acid detecting device 3 including a pipette 31, a PCR device 32 and a centrifuge 33 are disposed in the box structure 1. The centrifugal fan 22 vertically blows the filtered air into the working area A below, discharges the original air in the working area A, enables the air in the working area A to be always in a clean state, forms vertical airflow, blocks the diffusion of aerosol of a sample, and effectively prevents aerosol pollution.

A fluorescence PCR instrument (namely PCR equipment 32) and a centrifuge 33 are fixed on a platform at the bottom of the working area A, and power systems of all devices can be integrated into the equipment in the embodiment, so that the external connection state of an instrument power supply and the state of dirt collection and dirt collection in a conventional super clean workbench are improved. The outer surface is smooth, which is helpful for improving the effectiveness of disinfection and sterilization.

And a pipette bracket 7 is arranged on the left side of the working area A, so that a pipette 31 can be vertically placed instead of being horizontally placed on a bottom platform in a conventional ultra-clean workbench, and the tip of the pipette 31 is prevented from being polluted.

Work area A right side is provided with dedicated garbage bin 8, makes things convenient for used centrifuging tube, rifle head in the detection operation in time to retrieve to 8 openings of garbage bin are less, reduce the recontamination possibility.

The top of the working area A is provided with the ultraviolet sterilizing lamp 5, so that the sample can be sterilized in time after being detected every time, and the next batch of detection samples can be prevented from being polluted.

The middle part of the box body structure 1 of the embodiment is provided with a visible window 6. In particular to a glass window which is convenient for detection operation.

The operation door 4 of this embodiment is rotatably connected to the box structure 1 through a hinge 9, and the operation door 4 is turned up from bottom to top and is attracted to the box structure 1 through a magnet 10. In order to make the box structure 1 more stable, four bottom corners 11 are arranged at the bottom of the box structure 1.

The front surface of the box body structure 1 of this embodiment is further provided with a control panel 12, which is an electronic control unit such as a single chip microcomputer and a PLC controller, the control panel 12 is further integrated with an LED display screen, an entity and a virtual key, the control panel 12 is connected with each functional module through data lines to control the opening and closing of the centrifugal fan 22 in the airflow filtering device 2; controlling the on and off of the illuminating lamp in the working area A; controlling the ultraviolet sterilization lamp 5 in the working area A to be turned on and off; controlling the on and off of each device of the nucleic acid detection device 3 in the working area A; the LED display of the control panel 12 will display the on state of each module.

The working principle is as follows:

during work, the airflow filtering device 2 is opened, the equipment operation door 4 is opened, and a sample and reagents required by the test are put in. And (3) taking off the pipette 31 on the left pipette support 7, sleeving a gun head, opening the sample tube cover, sucking a sample required by the test, adding the sample into the test reagent, blowing and beating the sample up and down to be mixed uniformly, then covering the tube cover, putting the tube cover into a centrifuge 33, and centrifuging for 30 seconds. The used gun head is driven into the garbage can 8.

And opening the cover of the fluorescent PCR instrument, putting the centrifuged sample and test reagent mixed solution on a hole of the fluorescent PCR instrument (PCR equipment 32), covering the cover, and closing the equipment operation door 4. The working program is set on the control panel 12 and waits for the test result to come out.

After the test is completed, the equipment operation door 4 is opened, the cover of the fluorescence PCR instrument is opened, the test tube is taken out, the test tube is thrown into the garbage can 8, then the cover of the fluorescence PCR instrument is covered, and the garbage can 8 is taken out to be clean. And closing the equipment operation door 4, closing the airflow filtering device 2, starting the ultraviolet sterilizing lamp 5 for sterilization, and closing the ultraviolet sterilizing lamp 5 after half an hour.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an anti-pollution nucleic acid check out test set, is including detecting with the box structure, its characterized in that: the upper part in the box body structure is provided with an airflow filtering device, the lower part in the box body structure is provided with a nucleic acid detection device, and the front side of the box body structure is provided with an operation door; the airflow filtering device comprises a primary filter, a centrifugal fan and a high-efficiency filter which are sequentially arranged from top to bottom to form a vertical airflow duct.

2. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the nucleic acid detection device comprises a liquid transfer device, PCR equipment and a centrifugal machine which are arranged in a box body structure.

3. The contamination-preventive nucleic acid detecting apparatus according to claim 1, wherein: an ultraviolet sterilizing lamp is arranged above the nucleic acid detecting device.

4. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the middle part of the box body structure is provided with a visible window.

5. The contamination-preventive nucleic acid detecting apparatus according to claim 2, characterized in that: the lower part in the box body structure is also provided with a liquid transfer device bracket and a garbage can.

6. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the operation door is rotatably connected with the box body structure through a hinge, and the operation door is turned up from bottom to top and is attracted with the box body structure through a magnet.

7. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the front of the box body structure is also provided with a control panel, and the control panel is connected with the airflow filtering device and the nucleic acid detection device through data lines in a control mode.

8. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the lower part in the box body structure is a working area.

9. The contamination-preventive nucleic acid detecting apparatus according to claim 1, characterized in that: the height of the box body structure is 50-70 cm.