CN212610643U

CN212610643U - Vehicle-mounted nucleic acid detection specimen preparation system

Info

Publication number: CN212610643U
Application number: CN202020960596.0U
Authority: CN
Inventors: 谢孝民; 李丹; 熊磊; 谢正华; 陈才夫; 袁庆明; 王兆松
Original assignee: Shanghai 3D Medicines Co Ltd
Current assignee: Shanghai 3D Medicines Co Ltd
Priority date: 2020-05-29
Filing date: 2020-05-29
Publication date: 2021-02-26
Anticipated expiration: 2030-05-29

Abstract

The utility model relates to an on-vehicle nucleic acid testing sample preparation system through the whole negative pressure laboratory that will be used for nucleic acid testing sample preparation establish on vehicle platform, can at any time according to needs quick travel, nimble deployment to the area of needs most, can effectively improve hot spot area nucleic acid testing sample preparation ability through reasonable allotment, promotes nucleic acid detection efficiency, need not the repeated construction negative pressure laboratory simultaneously, avoids the wasting of resources.

Description

Vehicle-mounted nucleic acid detection specimen preparation system

Technical Field

The utility model relates to a pathogen detection equipment technical field especially relates to a vehicle-mounted nucleic acid detection sample preparation system.

Background

The novel coronavirus is an airborne virus with the characteristics of strong infectivity and strong pathogenicity, and often presents explosive diffusion infection in community people, thereby forming a very serious threat to the life health of people.

At present, no specific medicine exists for diseases such as pneumonia caused by the novel coronavirus, so that the early discovery and isolation of infected people become the most effective means for preventing and treating the novel coronavirus, and the currently most commonly used nucleic acid detection method is a screening means with high accuracy and reliable result.

At present, the sample collection for detecting the new coronavirus is mainly carried out in a hospital, the centralized collection of a large number of people is easy to cause cross infection, and a plurality of medical care personnel are infected; need control the air flow direction through pressure differential between the sample processing, use the negative pressure laboratory to avoid the sample to flow to the external world, cause environmental pollution, and current hospital negative pressure laboratory is limited in quantity, and when patient's quantity is more, need carry out sample processing fast on a large scale, current negative pressure laboratory is difficult to satisfy the demand.

SUMMERY OF THE UTILITY MODEL

For solving prior art's not enough, the utility model provides an on-vehicle nucleic acid testing sample preparation system through the whole establishment of negative pressure laboratory that will be used for nucleic acid testing sample preparation on vehicle platform, provides the nucleic acid testing sample preparation ability that can the quick travel disposed, can effectively improve hot spot area nucleic acid testing sample preparation ability through reasonable allotment, promotes nucleic acid detection efficiency, need not the repeated construction negative pressure laboratory simultaneously, avoids the wasting of resources.

In order to achieve the above object, the utility model discloses the technical scheme who adopts includes:

a vehicle-mounted nucleic acid detection specimen preparation system is characterized by comprising a buffer module, a nucleic acid processing module and a sample collection module, wherein the buffer module is isolated from the first airtight door and used for maintaining positive pressure, the nucleic acid processing module is used for maintaining negative pressure, and the sample collection module is arranged on the first airtight door;

the buffer module comprises positive pressure equipment, a vehicle-mounted power supply device, a first airtight door connected with the nucleic acid processing module and a second airtight door connected with the external environment;

the nucleic acid processing module comprises negative pressure equipment, an inactivation constant temperature box, a refrigerator, a biological safety cabinet, a nucleic acid extraction instrument, an operation table and power distribution equipment which is electrically connected with the vehicle-mounted power supply device and/or external power supply, and the side wall of the nucleic acid processing module is at least provided with an airtight transmission window connected with the external environment;

the sample collection module is arranged outside the nucleic acid processing module and is connected with the inside of the nucleic acid processing module through the airtight transmission window.

Further, the first airtight door and the second airtight door are rotating doors which are opened towards one side of the buffer module.

Furthermore, a vibration absorption connecting structure is arranged at the bottom of the operating platform; the shock absorbing attachment structure includes at least one horizontal shock absorbing spring.

Further, the biological safety cabinet is internally provided with negative pressure, and a centrifugal machine and a vortex oscillation instrument are arranged in the biological safety cabinet.

Further, the negative pressure value in the biological safety cabinet is lower than the negative pressure value maintained by the nucleic acid processing module.

Further, the nucleic acid treatment module also comprises a hand washing basin, a waste liquid cylinder, a waste garbage can and an autoclave.

Further, the buffer module and the nucleic acid processing module are also provided with an ultraviolet disinfection lamp and a ventilation device.

Further, an ultraviolet disinfection lamp is arranged in the airtight transfer window.

Further, the external power supply comprises a mains interface and/or a photovoltaic power supply arranged on the top outside the system.

Further, the system also comprises an independent air conditioning device.

The utility model has the advantages that:

adopt on-vehicle nucleic acid testing sample preparation system has realized establishing the complete negative pressure laboratory that has buffer module on vehicle platform, can accomplish the whole flows of nucleic acid testing sample preparation in this system, make nucleic acid testing sample preparation ability break away from the constraint of fixed laboratory, can be at any time as required quick travel, arrange in a flexible way to the area of needs most, make full use of current nucleic acid testing sample preparation ability, reduce interim negative pressure laboratory construction pressure, be the ideal solution of the large amount of detection demands of coping emergency.

Drawings

FIG. 1 is a schematic view of the top view of the internal structure of the embodiment of the system for preparing the nucleic acid detection specimen for vehicle of the present invention.

Fig. 2 is a schematic left side view of the internal structure of the embodiment of the present invention.

Fig. 3 is a right side view of the internal structure of the embodiment of the present invention.

Fig. 4 is a schematic rear view of an internal structure of the embodiment of the present invention.

Description of the figure numbering: 1-buffer module, 11-positive pressure equipment, 12-vehicle-mounted power supply device, 13-first airtight door, 14-second airtight door, 2-nucleic acid processing module, 21-negative pressure equipment, 22-refrigerator, 23-operation table, 231-inactivation thermostat, 232-biological safety cabinet, 233-nucleic acid extractor, 24-power distribution equipment, 25-airtight transfer window, 26-hand washing pool, 27-waste liquid cylinder, 28-waste garbage bin, 29-autoclave, 3-ultraviolet disinfection lamp, 4-air interchanger, 5-photovoltaic power supply, 6-air conditioning device and 7-sample collection module.

Detailed Description

For a clearer understanding of the present invention, reference will be made to the following detailed description of the embodiments with reference to the accompanying drawings.

Referring to fig. 1 to 4, the internal structure of an embodiment of the present invention is schematically illustrated, in which the buffer module 1 and the nucleic acid processing module 2 are sequentially disposed on a chassis of a van vehicle, and the buffer module 1 and the nucleic acid processing module 2 are completely air-tightly isolated from a cab of the vehicle to avoid contamination, but the system may also be disposed in other suitable movable structures such as a box trailer or a container. The buffer module 1 comprises a positive pressure device 11, an on-board power supply device 12 (such as a large-capacity lithium battery and/or a dedicated generator), and a first airtight door 13 connected with the nucleic acid processing module 2 and a second airtight door 14 connected with the external environment; the nucleic acid processing module 2 comprises a negative pressure device 21, a refrigerator 22 maintained at 4 ℃, a nucleic acid extractor 233, an operation table 23, an inactivation thermostat 231, a biological safety cabinet 232 maintained at negative pressure, a hand washing pool 26, a waste liquid cylinder 27, a waste garbage can 28, an autoclave 29 for waste liquid and 96-hole plate disinfection, and a power distribution device 24 electrically connected with the vehicle-mounted power supply device 12 and/or a mains supply and a roof photovoltaic power supply 5 for power supply, and the side wall of the nucleic acid processing module 2 is at least provided with an airtight transmission window 25 connected with the external environment; the sample collection module 7 is disposed in an external environment and connected to the inside of the nucleic acid processing module 2 through the airtight transfer window 25, and the collected sample can be safely transferred into the nucleic acid processing module 2 through the airtight transfer window 25, and the sample collection module 7 may be disposed at a position outside the nucleic acid processing module 2 close to the airtight transfer window 25 or may be separately disposed at an external environment far from the nucleic acid processing module 2 and transferred by a person skilled in the art according to actual needs and site conditions. The specific shape of the operation table 23 can be arbitrarily selected according to the need, for example, a U table, a corner table or a split type combination table can be selected, the nucleic acid extractor 233 and the biosafety cabinet 232 are preferably placed on the operation table 23, and a plurality of nucleic acid extractors 233 are preferably arranged in parallel on the operation table 23 for the purpose of improving the work efficiency; in order to reduce the influence of low-frequency vibration on the preparation of the specimen, a vibration absorption connecting structure is preferably arranged between the bottom of the operating platform 23 and the vehicle body, and at least one horizontal shock absorption spring is used for absorbing the vibration. The power distribution equipment 24 automatically distributes power supply for use through state monitoring of the vehicle-mounted power supply device 12 and connection conditions of commercial power and power supply of the roof photovoltaic power supply 5 and/or power supply of a vehicle engine, so that stable system power supply is ensured, and especially, stable power supply for a long time can be ensured for the refrigerator 22, so that the refrigerator 22 can continuously work for a long time without limitation. In order to ensure that the reagent preparation process does not pollute the external environment, the buffer module 1 and the nucleic acid processing module 2 are respectively manufactured by the positive pressure device 11 and the negative pressure device 21 to form a positive pressure environment and a negative pressure environment, and the first airtight door 13 and the second airtight door 14 of the revolving door structure can be closed by self through the pressure difference between the two modules and the pressure difference between the buffer module 1 and the external environment. Meanwhile, in order to improve the protection effect of the system, the ultraviolet disinfection lamp 3 can be preferably arranged in the buffer module 1, the nucleic acid processing module 2 and the airtight transfer window 25, so that the vehicle-mounted system can be disinfected and cleaned; the buffer module 1 and the nucleic acid processing module 2 are also provided with independent air exchange devices 4, preferably air exchange devices with filtering functions, and the air in the modules is kept clean; the system is also provided with an independent air conditioning device 6 different from the vehicle-mounted platform, so that pollution caused by mixed air conditioning is avoided.

The system provides the tertiary pressure safety guarantee of malleation buffering module 1, negative pressure nucleic acid processing module 2 and lower negative pressure biosafety cabinet 232, can thoroughly avoid harmful substance to spread to the external environment.

Use during the system, the staff enters buffer module 1 behind second airtight door 14, after disinfecting and wearing various safeguard procedures, gets into nucleic acid processing module 2 through first airtight door 13, carries out the sample preparation operation. After the sample is collected by the sample collection module 7, the sample is sterilized and transferred to the nucleic acid processing module 2 through the airtight transfer window 25. The detection personnel firstly inactivate the sample in an inactivation incubator 231 for a period of time, then transfer the inactivated sample to a biological safety cabinet 232 and add the sample into a 96-well plate. And then, putting the batch samples carried by the 96-well plate into the nucleic acid extractor 233 on the operation platform 23 for nucleic acid extraction, transferring the 96-well plate into the biological safety cabinet 232 again after extraction, adding the nucleic acid solution into the corresponding PCR tube, sealing the PCR tube, preserving the PCR tube, and transmitting the PCR tube through the airtight transmission window 25. The waste liquid (containing 96-well plates) remaining after extraction is put together in an autoclave 29 for inactivation, and the contents are discarded in a waste bin 28 after inactivation.

The buffer module 1 can provide necessary functions of changing clothes and sterilizing for people, and can also be preferably provided with a function of detecting rest of people, such as a toilet, a bathroom and the like, by utilizing a vacant position. The outside of the system can be provided with a retractable sunshade which provides more extended functions in the external environment.

On-vehicle nucleic acid testing sample preparation system both can the exclusive use, also can regard as a part to use with other portable laboratory structure combinations, form the nucleic acid testing laboratory of a complete flow.

The above description is only for the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A vehicle-mounted nucleic acid detection specimen preparation system is characterized by comprising a buffer module, a nucleic acid processing module and a sample collection module, wherein the buffer module is isolated from the first airtight door and used for maintaining positive pressure, the nucleic acid processing module and the sample collection module are used for maintaining negative pressure;

2. The system of claim 1, wherein the first and second airtight doors are rotating doors that open to one side of the cushion module.

3. The system of claim 1, wherein the console bottom is further provided with a shock absorbing attachment structure; the shock absorbing attachment structure includes at least one horizontal shock absorbing spring.

4. The system of claim 1, wherein the interior of the biosafety cabinet is under negative pressure, and the interior of the biosafety cabinet is provided with a centrifuge and a vortex shaker.

5. The system of claim 4, wherein the biosafety cabinet interior negative pressure value is lower than the negative pressure value maintained by the nucleic acid processing module.

6. The system of claim 1, wherein the nucleic acid processing module further comprises a hand sink, a waste tank, a waste bin, and an autoclave.

7. The system of claim 1, wherein the buffer module and the nucleic acid processing module are further provided with an ultraviolet disinfection lamp and a ventilator.

8. The system of claim 1, wherein an ultraviolet disinfection lamp is further disposed within the hermetic transfer window.

9. The system of claim 1, wherein the external power supply comprises a mains interface and/or a photovoltaic power supply disposed on top of the outside of the system.

10. The system of claim 1, further comprising a stand-alone air conditioning device.