WO2022088477A1 - Nucleic acid detector and nucleic acid detection method - Google Patents

Nucleic acid detector and nucleic acid detection method Download PDF

Info

Publication number
WO2022088477A1
WO2022088477A1 PCT/CN2020/140787 CN2020140787W WO2022088477A1 WO 2022088477 A1 WO2022088477 A1 WO 2022088477A1 CN 2020140787 W CN2020140787 W CN 2020140787W WO 2022088477 A1 WO2022088477 A1 WO 2022088477A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
reagent
assembly
plate
tube
Prior art date
Application number
PCT/CN2020/140787
Other languages
French (fr)
Chinese (zh)
Inventor
陈华云
杨迎宾
邹天桥
薛儒冰
肖湘文
刘淑园
吕婧
曾烨
Original Assignee
广州和实生物技术有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 广州和实生物技术有限公司 filed Critical 广州和实生物技术有限公司
Publication of WO2022088477A1 publication Critical patent/WO2022088477A1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Definitions

  • the present application relates to the technical field of biological detection, for example, to a nucleic acid detector and a nucleic acid detection method.
  • Nucleic acid hybridization technology is to immobilize specific probes on solid-phase materials respectively, and then hybridize the specific amplified product generated by nucleic acid amplification reaction with it, so that the sample to be tested will be with the probe with homologous sequence. Binding, because the sample to be tested has markers such as biotin, fluorescein, or digoxigenin, the probe points that bind to the sample to be tested have markers such as biotin, fluorescein, or digoxigenin, etc.
  • the hybridization signal can be displayed by the corresponding chemiluminescence reaction or fluorescence excitation.
  • This detection reaction can detect a variety of target sequences in one hybridization reaction, and has the characteristics of rapidity, simplicity, high sensitivity and strong specificity, especially in genotyping, gene mutation detection, pathogen detection, etc. It has unique advantages.
  • nucleic acid amplification based on traditional solutions, instruments and equipment for nucleic acid extraction and purification, nucleic acid amplification, and nucleic acid molecular hybridization that are commercially used in diagnosis are all discrete, such as sample preparation instruments and nucleic acid extraction instruments for nucleic acid extraction; PCR instrument or other nucleic acid amplification instrument, etc.; automated hybridization instrument for hybridization, etc.
  • the specific process of completing the entire experiment includes, but is not limited to, manually adding the sample to the automatic nucleic acid extractor or fully manual extraction, using the automatic nucleic acid extractor or fully manual extraction and purification of the biological sample, and manually transferring the purified nucleic acid solution to the laboratory.
  • Nucleic acid amplification instruments use nucleic acid amplification instruments to amplify the extracted nucleic acids, and then add the amplified products to an automatic hybridization instrument for final hybridization analysis.
  • Non-visual chemiluminescence methods also need to be equipped with a fluorescence scanner, etc. The whole experiment is either performed manually or concatenated, or the extraction and amplification, molecular hybridization, etc. are performed by different instruments respectively. This leads to the following problems:
  • the entire nucleic acid detection process requires highly qualified professional laboratory personnel to follow up the whole process, which increases labor costs and the possibility of human errors; at the same time, when processing possible medium and high infectious samples, it is important to the life safety of the laboratory personnel and the entire experiment.
  • the environment poses a biosecurity threat.
  • the entire nucleic acid detection process is carried out in stages, requiring manual intervention, which increases the uncontrollability and uncertainty of the test. At the same time, the test time is long, and the detection efficiency is low or restricted.
  • the entire nucleic acid detection process requires more than 10 kinds of equipment such as medium and high-speed centrifuges, water baths, pipettes, nucleic acid extraction and purification instruments, reagent dispensers, nucleic acid amplification instruments, hybridization instruments, and scanners. There are more than 15 kinds of supporting consumables, etc. At the same time, in order to meet the detection requirements, at least 30 different solutions are required, and the equipment and preparation of the entire reagent consumables and instruments are extremely complicated.
  • the entire nucleic acid detection process requires manual transfer of samples, various intermediate solutions, etc.
  • the operating environment is relatively open, which increases the risk of mutual contamination between samples and the risk of mutual contamination of solutions, resulting in the implementation of the entire nucleic acid detection.
  • Environmental requirements Extremely high; the instrument and consumables equipped at the same time are expensive, which is not suitable for large-scale promotion and application.
  • the present application provides a nucleic acid detector, which can improve nucleic acid detection efficiency and reduce nucleic acid detection costs.
  • the present application also provides a nucleic acid detection method, which can improve the nucleic acid detection efficiency and reduce the nucleic acid detection cost.
  • An embodiment provides a nucleic acid detector, including: a mounting frame; a reagent plate; nucleic acid amplification tubes, hybridization tanks, reaction tubes and reagent tubes for storing extraction reagents arranged on the reagent plate; a cover plate assembly, set on the mounting rack, and can move in the direction toward or away from the nucleic acid amplification tube to close or open the nozzle of the nucleic acid amplification tube; a heat treatment unit, arranged on the mounting rack, and arranged In order to heat or cool the liquid on the reagent plate; a pipetting device is arranged on the mounting frame, and is arranged to suck and transfer the liquid on the reagent plate; an oscillation unit is arranged on the installation frame The oscillating unit is arranged to drive the reagent plate to oscillate back and forth in a horizontal direction; and a magnetic attraction component is arranged on the mounting frame and can be moved toward or away from the reagent plate. moving in a direction, the magnetic attraction assembly is configured to magnetically
  • One implementation provides a nucleic acid detection method, which is applied to the nucleic acid detector as described above, and the nucleic acid detection method includes: pre-detection treatment, nucleic acid extraction and purification, nucleic acid amplification and hybridization reaction.
  • the pre-detection treatment includes: opening the cap of the nucleic acid amplification tube; and closing the mouth of the nucleic acid amplification tube by the cover plate assembly.
  • the nucleic acid extraction and purification is performed based on the magnetic bead method, and the nucleic acid extraction and purification includes: the pipetting device adds a sample to the reagent tube containing proteinase K and magnetic beads, and mixes it evenly; the pipetting device Pipetting the mixed solution of proteinase K, magnetic beads and sample liquid after mixing into a reaction tube containing the magnetic bead binding solution; the heat treatment unit regulates the temperature conditions required for the reaction of the reaction tube to lyse cells and release nucleic acids; and After the nucleic acid is released, the nucleic acid is adsorbed, washed and eluted on the magnetic beads through the reciprocating motion of the magnetic attraction component close to or away from the reagent plate and the liquid extraction and transfer of the pipetting device to obtain purified nucleic acid. nucleic acid.
  • the nucleic acid amplification reaction includes: the movement of the cover plate assembly to open the mouth of the nucleic acid amplification tube; the pipetting device sucks a certain amount of nucleic acid into the nucleic acid amplification tube; the movement of the cover plate assembly makes the nucleic acid amplification tube open. and start the nucleic acid amplification procedure to carry out the nucleic acid amplification reaction, during which the temperature conditions required for the nucleic acid amplification reaction are controlled by the heat treatment unit.
  • the hybridization reaction includes: moving the cover plate assembly to open the mouth of the nucleic acid amplification tube; the pipetting device extracting the amplification solution in the nucleic acid amplification tube into the hybridization tank; and the pipetting device sequentially extracting Different hybridization solutions are put into the hybridization tank, the temperature required for the reaction is regulated by the heat treatment module, and the oscillation unit drives the reagent plate to vibrate horizontally and reciprocally to realize the specific binding, washing and color development of the amplification product and the probe on the hybridization membrane. .
  • FIG. 1 is a schematic structural diagram of the nucleic acid detector provided in Embodiment 1 of the present application.
  • FIG. 2 is a schematic diagram of the internal structure of the nucleic acid detector provided in Embodiment 1 of the present application;
  • FIG. 3 is a schematic diagram of a disassembled structure of the nucleic acid detector provided in the first embodiment of the application;
  • FIG. 4 is a schematic view of the split structure of the housing provided in Embodiment 1 of the present application.
  • FIG. 5 is a schematic structural diagram of the reagent plate provided in Embodiment 1 of the present application.
  • FIG. 6 is a schematic structural diagram of the oscillating heating assembly and the reagent plate provided in Embodiment 1 of the present application;
  • FIG. 7 is a schematic structural diagram of the oscillating heating assembly provided in Embodiment 1 of the present application from a viewing angle;
  • Fig. 8 is a partial enlarged view at I place in 7;
  • FIG. 9 is a schematic view of the disassembled structure of the oscillating heating assembly provided in the first embodiment of the application.
  • FIG. 10 is a schematic structural diagram of the cover plate assembly provided in Embodiment 1 of the present application from a viewing angle;
  • FIG. 11 is a schematic structural diagram of the cover plate assembly provided in Embodiment 1 of the present application from another perspective;
  • FIG. 12 is a schematic view of the disassembled structure of the cover plate assembly provided in the first embodiment of the application.
  • FIG. 13 is a schematic structural diagram of the magnetic attraction assembly provided in Embodiment 1 of the present application from a viewing angle;
  • FIG. 14 is a schematic structural diagram of the magnetic attraction assembly provided in Embodiment 1 of the present application from another perspective;
  • FIG. 15 is a schematic diagram of a disassembled structure of the magnetic attraction component provided in Embodiment 1 of the present application;
  • Fig. 16 is a partial enlarged view at J in Fig. 15;
  • FIG. 17 is a schematic structural diagram of the first liquid pipetting mechanism provided in Embodiment 1 of the present application from a viewing angle;
  • FIG. 19 is a schematic diagram of a disassembled structure of the first pipetting mechanism provided in Embodiment 1 of the application;
  • FIG. 20 is a schematic structural diagram of the second pipetting mechanism provided in Embodiment 1 of the application from one viewing angle;
  • FIG. 21 is a schematic structural diagram of the second pipetting mechanism provided in Embodiment 1 of the application from another perspective;
  • FIG. 22 is a schematic diagram of a disassembled structure of the second pipetting mechanism provided in Embodiment 1 of the present application;
  • FIG. 24 is a schematic diagram of the connection relationship between the liquid pump and the reagent needle pipeline assembly provided in the first embodiment of the application.
  • Oscillation heating assembly 31. Heat treatment unit; 311, Nucleic acid amplification tube heating block; 312, Reaction tube heating block; 313, Extraction reagent heating block; 314, Hybridization tank heating plate; 315, Heating film; 316, Heat dissipation unit 3161, radiator; 31611, vertical plate part; 31612, horizontal plate part; 31613, fin part; 3162, cooling fan; 317, refrigeration unit; 321, support plate; 322, X-direction guide assembly; 3221, X-direction guide rail; 3222, X-direction slider; 323, limit block; 324, fixed bracket; 33, heat insulation unit; 331, heat insulation block; 332 , Insulation column; 333, Insulation carrier plate; 334, Insulation pillar; 335, Insulation board;
  • Reagent plate 41. Main board; 411. Amplification well position; 412, Hybridization tank; 413, Bayonet; 414, Tip chamber; 415, Waste liquid tank; , sample tube; 45, pipette tip;
  • cover plate assembly; 51 cover seal assembly; 511, top cover plate; 512, vertical connecting plate; 513, horizontal connecting plate; 5131, first horizontal plate part; 5132, second horizontal plate part; 5133, optical axis hole; 514, cover seal; 52, cover drive assembly; 521, cover motor; 522, cover screw; 523, cover nut seat; 53, cover motor seat; 531, mounting plate; 5311, through plate mouth; 532, mounting seat; 533, motor support plate; 54, cover plate guide assembly; 541, optical axis seat; 542, optical axis; 543, linear bearing;
  • Magnetic suction assembly 61, Magnetic rod assembly; 611, Magnetic rod; 612, Mounting strip; 6121, Mounting hole; 6122, Piercing; 6123, Positioning part; 613, Mounting cross plate; 6131, Positioning slot; 62, Magnetic rod drive assembly; 63, magnetic guide assembly; 64, magnetic motor seat;
  • the first pipetting mechanism 71, the first reagent needle unit; 711, the first reagent needle; 712, the first reagent needle holder; 72, the first horizontal displacement unit; 721, the first horizontal drive assembly; 722, the connection 723, motor fixing plate; 73, first vertical displacement unit; 731, adapter plate; 732, first vertical drive assembly; 74, first horizontal guide assembly; 75, first vertical guide assembly; 76 , buffer block; 77, guide rail plate;
  • the second pipetting mechanism 81.
  • Liquid pump group 10. Syringe pump;
  • Liquid level sensing assembly 40. Reagent bottle; 50. Consumables panel; 501, Reagent plate mouth; 60, Display screen; 70, Pipeline assembly; 701, Blow pipe; pipe; 704, separate liquid pipe; 705, separate waste liquid inlet pipe; 706, main waste liquid inlet pipe; 707, waste liquid outlet pipe; 80, waste liquid bottle.
  • connection should be understood in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integrated ; It can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between the two elements.
  • connection may be a fixed connection, a detachable connection, or an integrated ; It can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between the two elements.
  • a first feature "on” or “under” a second feature may include direct contact between the first and second features, or may include the first and second features Not directly but through additional features between them.
  • the first feature being “above”, “over” and “above” the second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is level higher than the second feature.
  • the first feature is “below”, “below” and “below” the second feature includes the first feature being directly below and diagonally below the second feature, or simply means that the first feature has a lower level than the second feature.
  • FIG. 1 is a schematic structural diagram of a nucleic acid detector provided by an embodiment of the present application
  • FIG. 2 is a schematic diagram of an internal structure of a nucleic acid detector provided by an embodiment of the present application
  • FIG. 3 is a schematic structural diagram of a disassembled nucleic acid detector provided by an embodiment of the present application.
  • this embodiment provides a nucleic acid detector, which can be applied to molecular diagnosis of biological samples such as genotyping, gene mutation detection, pathogen detection, etc., to realize sample pretreatment, nucleic acid extraction and purification Integration and automation of processes such as amplification and hybridization detection.
  • the nucleic acid detector provided in this embodiment includes a casing 1 and a detection body disposed in the casing 1 .
  • the housing 1 has a relatively closed accommodating space, and the detection body is arranged in the accommodating space.
  • the housing 1 is set to seal the detection body, so that the nucleic acid detection work is performed in a relatively closed and clean environment, so as to reduce the contamination of the reagents by the external environment.
  • the detection main body includes a reagent plate 4, an installation frame 2, an oscillating heating assembly 3, a magnetic suction assembly 6, a cover plate assembly 5 and a pipetting device arranged on the installation frame 2.
  • the reagent plate 4 is arranged on the oscillating heating assembly 3 and oscillates relatively.
  • the heating assembly 3 is fixed.
  • the reagent plate 4 is a carrier for all reactions such as sample pretreatment, sample nucleic acid extraction and purification, sample nucleic acid amplification and molecular hybridization;
  • the mounting frame 2 is arranged to provide fixation and support for the structure in the detection body;
  • the cover plate assembly 5 It is set to automatically close and open the nucleic acid amplification tube set on the reagent plate 4;
  • the magnetic suction assembly 6 is set to perform magnetic attraction and magnetization during the nucleic acid extraction process;
  • the oscillating heating component 3 is set to be set during the nucleic acid detection process. , realize the heating and cooling operation of the liquid on the reagent plate 4, and realize the liquid shaking operation in the nucleic acid hybridization process;
  • an XYZ coordinate system is established with the directions shown in Figure 2, wherein the X direction is the arrangement direction of the reagent plate 4, the Z direction is the height direction of the nucleic acid detector, and the Y direction is set horizontally and determined according to the right-hand rule.
  • FIG. 4 is a schematic view of the disassembled structure of the housing 1 provided by the embodiment of the application.
  • the housing 1 has a hexahedral structure as a whole, and includes a main housing 11.
  • the main housing 11 is provided with an opening, and the housing 1 It also includes a door body disposed at the opening and capable of opening and closing relative to the main casing 11 .
  • the door body includes a first front door 12 and a second front door 13 on the front side of the housing 1.
  • the first front door 12 and the second front door 13 are arranged side by side along the Y direction, and can be individually Opening and closing.
  • the first front door 12 is set corresponding to the position of the reagent plate 4 to facilitate the taking and placing of the reagent plate 4 in the accommodating space
  • the second front door 13 is set corresponding to the reagent bottle storage area in the nucleic acid detector to facilitate pure water, hybridization reagents Wait for the placement and replacement of the bottled liquid in the holding space.
  • the upper end of the first front door 12 is pivotally connected to the main casing 11 so that the first front door 12 can be rotated and opened and closed relative to the main casing 11 , and the second front door 13 adopts a push-and-pop door structure.
  • the first front door 12 can also be connected to the main casing 11 by other sides, and the second front door 13 can also be connected to the main casing 11 by rotating and closing.
  • the opening form of the second front door 13 and the connection structure with the main casing 11 are not specifically limited.
  • the rear side of the pair of the main casing 11 and the front door is also provided with a disassembly port for the detection body to pass through.
  • Backdoor 14 In this embodiment, the rear door 14 includes two door bodies arranged side by side along the Y direction, and each door body can be disassembled separately to improve the convenience of disassembly and assembly, and facilitate the maintenance and replacement of the local structure of the detection body. In one embodiment, the rear door 14 may also include only one door body or multiple door bodies.
  • the casing 1 in order to regulate and control the nucleic acid detector, is provided with a display screen 60 configured to realize human-computer interaction.
  • the operation interface controls the operation of the nucleic acid detector, and the display screen 60 can also display the operation state of the nucleic acid detector during the operation of the nucleic acid detector.
  • an installation opening 131 is formed through the second front door 13 , and the display screen 60 is embedded in the installation opening 131 and is electrically connected to the controller disposed inside the housing 1 .
  • the main casing 11 Since there are heating and exothermic processes in the nucleic acid detection process, in order to promote the dissipation of heat in the accommodation space, the main casing 11 is provided with heat dissipation grooves on the opposite sides and the bottom along the Y direction to avoid heat accumulation in the accommodation space .
  • a frame 15 is protruded from the inner bottom surface of the main casing 11 , and the upper surface of the frame 15 forms a support surface configured to carry the detection body.
  • the frame 15 is provided with a waste liquid collection tank 16, which is configured to collect waste liquid generated during the nucleic acid detection process, so as to facilitate the waste liquid to be discharged from the outside of the nucleic acid detector.
  • the bottom of the waste liquid collection tank 16 is provided with a discharge port, the discharge port is sealed and inserted with a discharge pipe, and the discharge pipe is arranged to discharge the waste liquid in the waste liquid collection tank 16 to an external waste liquid bottle, so as to facilitate the disposal of the waste liquid. After intensive treatment, it is discharged to the external environment.
  • the mounting frame 2 includes a base plate 21 parallel to the XY plane and a support frame 22 disposed above the base plate 21 .
  • the support frame 22 is in a U-shaped structure with an opening facing the base plate 21 , and the support frame 22 includes a Y direction.
  • the provided cross beam 221 and the vertical beam 222 provided at both ends of the cross beam 221 and connected between the cross beam 221 and the bottom plate 21 along the Z direction.
  • the cover plate assembly 5, the oscillating heating assembly 3 and the magnetic suction assembly 6 are all arranged on the bottom plate 21, the pipetting device is arranged on the support frame 22, and the U-shaped structure of the support frame 22 is arranged to facilitate the pipetting device along the Y direction. run.
  • the lower surface of the bottom plate 21 is protruded downward with a supporting leg 23, and at least two supporting legs 23 are arranged side by side along any direction in the X direction and the Y direction.
  • the frame 15 of the housing 1 An installation groove is formed on the upper part, and the support leg 23 extends into the installation groove, and the lower end of the support leg 23 is in contact with the groove bottom of the installation groove. This arrangement can reduce the contact area between the bottom plate 21 and the frame 15 while ensuring the support stability of the mounting frame 2 .
  • the nucleic acid detector further includes a consumables panel 50 , a plurality of reagent plate ports 501 are arranged side by side along the Y direction on the consumables panel 50 , and the reagent plate ports 501 extend along the X direction, as shown in FIG. 5 .
  • the reagent plate 4 includes a carrying main board 41 extending along the X direction, and the carrying main board 41 is provided with an amplification hole 411 configured to place a nucleic acid amplification tube , the hybridization tank 412 set to place the hybridization solid phase material, the pipette cavity 414 set to place the pipette tip, the reagent tube 42 set to place the extraction reagent, and the reaction tube 43 set to carry out the extraction reaction, the hybridization tank 412, the reagent tube 42 and the reaction tube 43 are respectively opened at the upper ends, the lower surface of the carrying main board 41 is placed on the consumables panel 50, and the nucleic acid amplification tube, the hybridization tank 412, the reaction tube 43 and the reagent tube 42 extends into the reagent plate port 501 .
  • the amplification hole 411 is arranged at one end of the reagent plate 4 , which facilitates the cover plate assembly 5 to seal and open the opening of the nucleic acid amplification tube and reduce structural interference.
  • the carrier main board 41 is also provided with a bayonet 413 which is configured to be clamped to the cap of the nucleic acid amplification tube.
  • the upper end of the bayonet 413 is open and connected to the amplification hole.
  • the upper end of 411 is open and connected, and the tube cover is opened relative to the tube body of the nucleic acid amplification tube and the shell is snapped into the bayonet 413 to prevent the tube cover from turning over.
  • the hybridization tank 412, the tip chamber 414, the reagent tube 42 and the reaction tube 43 are arranged in sequence along the X direction in the direction away from the amplification hole 411.
  • the arrangement shown in FIG. 5 is an exemplary structure. The arrangement positions of each hole, chamber and tube on the plate 4 can be exchanged, which is not specifically limited in this embodiment.
  • the bottom of the pipette cavity 414 is provided with a plurality of pipettes 45 along the X direction, and the pipettes 45 are arranged to store a plurality of pipettes, so as to ensure the number of pipettes that can be replaced during the nucleic acid detection process. Meet the needs of use and reduce the pollution between the detection reagents.
  • the number of suction tips that can be placed in the tip chamber 414 is three, but the present application is not limited to this.
  • the number of reagent tubes 42 may be specifically set according to the type of extraction reagent required for the nucleic acid detection item to be performed, and the number of reagent tubes 42 may be but not limited to four.
  • the carrying main board 41 is provided with a sample tube 44, and the sample tube 44 is set to store the sample.
  • the sample tube 44 By setting the sample tube 44 to store the sample separately, it can be applied to the nucleic acid detection project in which the extraction reagent is added first and then the sample is added. Applicability of nucleic acid detectors.
  • the sample can also be placed directly in the reaction tube 43 .
  • the carrying main board 41 is provided with a waste liquid tank 415, and the waste liquid tank 415 is set to receive the waste liquid, so as to reduce the distance that the pipetting device needs to move during the waste liquid removal process, and facilitate the transfer of the waste liquid. remove operation.
  • the waste liquid can also be directly sucked into a waste liquid bottle or a waste liquid storage tank outside the reagent plate 4 .
  • the upper end surface of the reagent plate 4 is also plastic-sealed with a sealing film, and the sealing film can be a plastic film, an aluminum film or a paper film. And the plastic sealing film can be torn off relative to the carrying main board 41, so that after the reagent plate 4 is put into the housing 1, the normal nucleic acid detection operation can be performed.
  • FIG. 6 is a schematic structural diagram of the oscillating heating assembly 3 and the reagent plate 4 provided by the embodiment of the present application
  • FIG. 7 is a schematic structural diagram of the oscillating heating assembly 3 provided by the embodiment of the present application from a viewing angle
  • FIG. 8 is the position I in FIG. 7
  • 9 is a schematic view of the split structure provided by the embodiment of the present application, as shown in FIGS.
  • the oscillating heating assembly 3 is arranged on the bottom plate 21 and is located below the beam 221, and a plurality of reagent plates 4 along the The oscillating heating assemblies 3 are arranged side by side in the Y direction, and the oscillating heating assemblies 3 are arranged to heat the liquid in the reagent plate 4 or drive the reagent plate 4 to reciprocate along the X direction. In one embodiment, only one reagent plate 4 may be provided.
  • the oscillating heating assembly 3 includes a heat treatment unit 31 , a heat insulating unit 33 and an oscillation unit 32 arranged in sequence from top to bottom, and a reagent plate 4 is fixed above the heat treatment unit 31 .
  • Using the heat insulation unit 33 to isolate the heat treatment unit 31 and the oscillation unit 32 can improve the treatment efficiency of the heat treatment unit 31 , and at the same time, prevent the heat from being transferred downward to the oscillation unit 32 and ensure the use reliability of the oscillation unit 32 .
  • the heat treatment unit 31 includes a plurality of heating units arranged side by side and at intervals along the X direction, and each heating unit extends along the Y direction.
  • the plurality of heating units include a nucleic acid amplification tube heating block 311 configured to heat the nucleic acid amplification tube, a hybridization tank heating plate 314 configured to heat the hybridization tank 412 , and a reaction tube 43 configured to heat
  • the reaction tube heating block 312 for heating and the extraction reagent heating block 313 configured to heat the extraction reagent, and the nucleic acid amplification tube heating block 311, the hybridization tank heating plate 314, the reaction tube heating block 312 and the extraction reagent heating block 313 are The arrangement positions in the X direction correspond to the positions of the amplification wells 411 , the hybridization tank 412 , the reaction tubes 43 and the reagent tubes 42 on the reagent plate 4 one by one.
  • a plurality of reagent tubes 42 are provided, and the extraction reagent heating block 313 is provided corresponding to one of the reagent tubes 42 in the middle, so that heat can be diffused to the remaining reagent tubes 42 around.
  • the number of extraction reagent heating blocks 313 may also correspond to the number of reagent tubes 42 .
  • each heating block is provided with a tube jack 319 configured to be inserted into the corresponding tube structure.
  • the nucleic acid amplification tube, the reagent tube 42 and the reaction tube 43 are inserted into the tube insertion holes 319 of the corresponding heating block, and the bottom of the hybridization tank 412 abuts on the heating plate 314 of the hybridization tank.
  • This arrangement can improve the heating efficiency of each heating unit to the corresponding tube or groove structure, reduce heat loss, and further ensure the relative stability of the position between the reagent plate 4 and the oscillating heating assembly 3 .
  • a heating film 315 is disposed on one side of each heating block along the length direction, and the heating film 315 is configured to be connected to a power source to provide a heat source for the corresponding heating block, that is, to heat the heating block.
  • the heating film 315 is disposed on the side of the heating block parallel to YZ, so as to prevent the heating film 315 from directly contacting the heat insulating unit 33 located below.
  • the structures of the heating film 315 and the heating plate that can be electrically heated are conventional structures in the field, and will not be repeated here.
  • each heating block is provided with a plurality of tube insertion holes 319 along the Y direction, and the number of tube insertion holes 319 on each heating block is One-to-one correspondence with the number and position of the reagent plates 4, so as to realize the synchronization of multiple nucleic acid detection experiments.
  • the tube insertion holes 319 on the nucleic acid amplification tube heating block 311 and the extraction reagent heating block 313 are cylindrical through holes or blind holes, and the upper open end can be provided with a chamfer to facilitate tube insertion.
  • the cross-sectional area of the tube insertion hole 319 on the reaction tube heating block 312 is an arc-shaped structure with an opening away from the nucleic acid amplification tube heating block 311 , and the tube insertion hole 319 penetrates the upper and lower ends and one side of the reaction tube heating block 312 wall, in order to facilitate the dissipation of heat, and help to reduce the weight of the reaction tube heating block 312, and at the same time, the pipe jack 319 on the reaction tube heating block 312 is set to the above structure, which is also beneficial for the magnetic attraction assembly 6 to the reaction tube 43.
  • the reacting liquid of the ferromagnetic material undergoes magnetic attraction and expulsion reactions, thereby reducing the structural obstacle between the magnetic attraction component 6 and the reaction tube 43 .
  • a weight-reducing groove 3110 is provided between two adjacent pipe insertion holes 319 to reduce the overall weight of the heating block, thereby reducing the overall weight of the oscillating heating assembly 3 .
  • the weight reduction groove 3110 penetrates the heating block in the Z direction.
  • a refrigeration unit 317 is provided on one side of the nucleic acid amplification tube heating block 311 along its length direction, and the refrigeration unit 317 is set to heat the nucleic acid amplification tube.
  • Block 311 performs a cooling process to cool the reagents in the nucleic acid amplification tube.
  • the cooling unit 317 includes a cooling sheet disposed opposite to the heating film 315 .
  • the structure and refrigeration principle of the refrigerating sheet are conventional settings in the field, and are not repeated in this embodiment.
  • a plurality of cooling sheets are arranged side by side along the length direction of the nucleic acid amplification tube heating block 311 to satisfy the cooling effect on the entire nucleic acid amplification tube heating block 311 .
  • a heat dissipation unit 316 is further provided on one side of the heating block 311 of the nucleic acid amplification tube.
  • the heat dissipation unit 316 includes a radiator 3161 and a heat dissipation fan 3162 that are stacked up and down, and the heat dissipation fan 3162 is connected to the lower part of the heat sink 3161 .
  • the radiator 3161 includes a vertical plate portion 31611 parallel to the YZ plane, a horizontal plate portion 31612 arranged horizontally and connected to the vertical plate portion 31611 , and a fin portion 31613 parallel to the vertical plate portion 31611 and connected to the horizontal plate portion 31612 .
  • the vertical plate portion 31611 is detachably connected to the nucleic acid amplification tube heating block 311 and is located on the opposite side of the heating film 315, and the cooling plate is sandwiched between the vertical plate portion 31611 and the nucleic acid amplification tube heating block 311; the vertical plate portion Both sides of the 31611 along the vertical direction are provided with horizontal plate portions 31612, the horizontal plate portions 3162 extend in a direction away from the nucleic acid amplification tube heating block 311, and the horizontal plate portion 31612 located below is detachably connected to the cooling fan 3162;
  • the sheet portions 31613 are connected between the two horizontal plate portions 31612, and are arranged side by side along the X-direction at intervals to enhance the heat dissipation effect.
  • a plurality of horizontal plate portions 31612 are arranged at intervals along the length direction of the nucleic acid amplification tube heating block 311 to increase the heat dissipation effect, and a cooling fan is detachably connected to each horizontal plate portion 31612 located below. 3162. This arrangement is more conducive to improving the heat dissipation effect.
  • the horizontal plate portion 31612 can also be provided with a whole piece along the length direction of the nucleic acid amplification tube heating block 311 .
  • the heat insulating unit 33 includes a plurality of heat insulating sub-units respectively arranged below the heating unit, the heat insulating sub-units are detachably connected to the corresponding heating units, and are arranged to perform heat insulating treatment on each heating unit to improve the heat insulating effect, and The heat loss at each heating block can be avoided, and the heating effect and heating efficiency of each heating unit during heating can be improved.
  • the heat insulating subunit located under the nucleic acid amplification tube heating block 311 and the extraction reagent heating block 313 is the heat insulating block 331
  • the heat insulating subunit located under the reaction tube heating block 312 is the heat insulating plate 335
  • the heat insulating plate 335 Both the heat insulating block 331 and the heat insulating block 331 are made of heat insulating material, and each heat insulating subunit extends along the Y direction to cover the length range of the corresponding heating block.
  • the heat insulating block 331 or the heat insulating plate may be provided with a positioning groove for positioning the heating block.
  • the thermal insulation subunit below the hybridization tank heating plate 314 includes a plurality of thermal insulation columns 332, the thermal insulation columns 332 are arranged vertically, and are arranged in multiple groups at intervals along the Y direction, and each group includes at least two arranged at intervals along the X direction. Insulation post 332. In one embodiment, the insulating posts 332 are made of insulating material.
  • the heat insulating unit 33 further includes a heat insulating support plate 333 located below all the heat insulating subunits, and each heat insulating subunit is arranged on the heat insulating support plate 333 and is detachably connected with the heat insulating support plate 333 .
  • the heat insulating unit 33 further includes heat insulating struts 334 located under the heat insulating support plate 333.
  • the heat insulating struts 334 are spaced in a plurality of groups along the Y direction, and each group at least includes an area along the X direction.
  • the plurality of thermal insulation pillars 334 are arranged at intervals in the direction.
  • the disposition of the heat insulating support 334 can increase the gap between the heat treatment unit 31 and the oscillating unit 32 , and can promote the flow of air between the heat insulating carrier plate 333 and the oscillating unit 32 to facilitate the diffusion of heat.
  • the thermal insulation pillars 334 and the thermal insulation carrier plate 333 are made of thermal insulation materials, but may also be made of materials with low thermal conductivity.
  • the oscillation unit 32 includes a support plate 321 parallel to the XY plane, an X-direction guide assembly 322 located below the support plate 321 and an oscillation drive unit (not shown) configured to drive the support plate 321 to reciprocate in the X direction.
  • the lower ends of the heat insulating pillars 334 are detachably connected to the support plate 321 .
  • the support plate 321 is provided with a plurality of weight-reducing holes.
  • the X-direction guide assembly 322 is disposed between the support plate 321 and the bottom plate 21 , and is configured to guide the support plate 321 relative to the bottom plate 21 along the X direction.
  • the X-direction guide assembly 322 includes an X-direction guide rail 3221 arranged on the bottom plate 21 along the X-direction and an X-direction sliding block 3222 arranged on the lower surface of the support plate 321 and slidably matched with the X-direction guide rail 3221.
  • the directional guide rail 3221 and the X-directional sliding block 3222 reference may be made to the structure of the linear guide rail in the related art, which will not be repeated here.
  • at least two groups of the X-direction guide components 322 are arranged at intervals along the Y-direction.
  • limit blocks 323 are provided at both ends of the X-direction guide rail 322, and the X-direction slider 3222 contacts the limit block 323 when sliding to the end of the X-direction guide rail 322.
  • the limiting block 323 is made of elastic material such as rubber, so as to avoid vibration, deformation or loud noise caused by the hard collision between the slider and the limiting block 323 while realizing the limiting.
  • the oscillating driving unit includes an X-direction screw motor (not shown), the fixed end of the X-direction screw motor is fixedly connected to the base plate 21 , and the driving end of the X-direction screw motor is connected to the support plate 321 . Since the dimension of the support plate 321 along the Y direction is relatively long, in order to improve the driving stability and reliability, one side of the support plate 321 is provided with a fixing bracket 324, and at least two fixing brackets 324 are arranged at intervals along the Y direction. 324 is connected to the same adapter arranged along the Y direction, and the adapter is connected to the X-direction screw motor. The arrangement of the adapter and the fixing bracket 324 can be elevated and set to the connection position with the X-direction screw motor, which improves the driving reliability and facilitates the setting of the X-direction screw motor.
  • the reciprocating stroke of the support plate 321 along the X direction is 10-20 mm.
  • the oscillating heating assembly 3 further includes a displacement detecting assembly 20 configured to detect the movement and displacement of the oscillating heating assembly 3 along the X direction.
  • the displacement detection assembly 20 includes a photoelectric switch 201 disposed on one side of the support plate 321 and a photoelectric induction sheet 202 disposed on the bottom plate 21 .
  • the structure and principle of detecting displacement by the photoelectric switch 201 are conventional settings in the field, and will not be repeated here.
  • other detection devices that can be used to detect the oscillating displacement such as a grating ruler, a distance sensor, etc., may also be provided.
  • the oscillating heating assembly 3 Since the oscillating heating assembly 3 generates heat with the bottom plate 21 during rapid oscillation, in order to avoid heat accumulation, at least one of the support plate 321 and the bottom plate 21 is provided with a fan at a position corresponding to the X-direction guide assembly 322 for heat dissipation.
  • FIG. 10 is a schematic structural diagram of the cover plate assembly 5 provided by the embodiment of the application from one perspective
  • FIG. 11 is a schematic structural diagram of the cover plate assembly 5 provided by the embodiment of the application from another perspective
  • FIG. 12 is an embodiment of the application Provided is a schematic diagram of the disassembled structure of the cover plate assembly 5.
  • the cover plate assembly 5 is arranged on one side of the oscillating heating assembly 3, and is located outside the end of the reagent plate 4 where the amplification hole position 411 is arranged. , set to close and open the opening of the nucleic acid amplification tube set in the amplification hole position 411 .
  • the cover plate assembly 5 includes a cover plate assembly 51 configured to close the opening of the nucleic acid amplification tube and a cover plate drive assembly 52 configured to drive the cover plate assembly 51 to move toward or away from the amplification hole 411 .
  • the cover plate driving assembly 52 drives the cover plate assembly 5 to move in the vertical direction, so as to improve the convenience of closing or opening the opening of the nucleic acid amplification tube.
  • the cover plate driving component 52 may also drive the cover sealing component 51 to rotate or move tilted relative to the horizontal direction, so that the cover sealing component 52 is in the first position of sealing the mouth of the nucleic acid amplification tube and starts nucleic acid amplification Toggle between the second position of the nozzle of the pipe.
  • the cover assembly 51 includes a cover bracket and a cover seal 514.
  • the cover bracket includes a vertically connected top cover 511 and a vertical connection plate 512.
  • the top cover 511 is horizontally arranged and located directly above the amplification hole 411, and is vertically connected.
  • the upper side of the plate 512 is connected to the top cover plate 511
  • the lower side of the vertical connecting plate 512 is connected to the cover plate driving assembly 52
  • the vertical connecting plate 512 is located outside one end of the reagent plate 4 .
  • the cover seal 514 is disposed on the inner surface of the top cover plate 511 and away from the side of the vertical connection plate 512, and the cover seal 514 is disposed facing the amplification hole 411, so that the cover seal 514 moves downward to be positioned in the amplification hole
  • the opening of the nucleic acid amplification tube at position 411 is closed, and the opening of the nucleic acid amplification tube can be opened when moving upward.
  • the cover sealing member 514 is made of materials such as silica gel, PE material, PP material, which cannot adsorb nucleic acid and has a certain elasticity, so as to achieve tight sealing of the opening.
  • the cover seal 514 has a sheet-like structure, which can reduce the size of the cover seal 514 . Further, the cover seal 514 extends along the Y direction and can cover the amplification wells 411 of the plurality of reagent plates 4 , so as to facilitate the setting of the cover seal 514 .
  • the number of amplification holes 411 that can be covered by a single cover seal 514 can be determined according to the number of nucleic acid detection tests that the nucleic acid detector can perform synchronously at one time, so as to facilitate the disassembly and replacement of the cover seal 514 .
  • the cover seal assembly 51 further includes a horizontally arranged horizontal connecting plate 513.
  • the first side of the horizontal connecting plate 513 is connected to the lower side of the vertical connecting plate 512.
  • the second side of 513 extends in a direction away from the reagent plate 4 .
  • the cover drive assembly 52 includes a cover motor 321 , a cover screw 522 connected to the output end of the cover motor 521 and arranged vertically, and a cover nut sleeved on the cover screw 522
  • the seat 523, the fixing part of the cover motor 521 is connected with the mounting frame 2, and the cover nut seat 523 is connected with the horizontal connecting plate 513.
  • the cover assembly 5 further includes a cover motor base 53 .
  • the cover plate motor seat 53 includes a mounting plate 531 parallel to the YZ plane.
  • the side of the mounting plate 531 away from the top cover plate 511 is provided with two mounting seats 532 at intervals along the Y direction, and each mounting seat 532 is detachably connected to the bottom plate 21 , in order to improve the connection stability with the bottom plate 21 .
  • a horizontally arranged motor support plate 533 is connected to the mounting plate 531 .
  • the motor support plate 533 is perpendicular to the mounting plate 531 and is detachably connected to the fixed cover motor 521 .
  • the mounting plate 531 is also provided with an escape hole for the cover plate screw 522 to pass through.
  • the mounting plate 531 is provided with a plate penetration opening 5311 therethrough.
  • the horizontal connecting plate 513 includes a first horizontal plate portion 5131 and a second horizontal plate portion 5132 connected along the X direction, the first side of the first horizontal plate portion 5131 is connected with the vertical connecting plate 512, the second horizontal plate portion 5131 The side is connected with the second transverse plate portion 5132, the length of the second transverse plate portion 5132 in the Y direction is greater than the length of the first transverse plate portion 5131 in the Y direction, and the first transverse plate portion 5131 is penetrated through the plate hole 5311 and can Vertically lift and lower in the hole 5311.
  • the cover sealing assembly 51 includes a cover plate guide assembly 54 for guiding the Z direction movement of the cover sealing assembly 51 .
  • the cover guide assembly 54 includes two optical axis seats 541 arranged on the mounting plate 531 at intervals in the vertical direction and an optical axis 542 arranged vertically. Both ends of the optical axis 542 are respectively inserted into the two optical axis seats 541 .
  • the second horizontal plate portion 5132 is provided with an optical axis hole 5133 , and the optical axis 542 passes through the optical axis hole 5133 , so that the horizontal connecting plate 513 can be vertically lifted and lowered along the optical axis 542 .
  • a linear bearing 543 is connected to the position of the horizontal connecting plate 513 corresponding to the optical axis hole 5133, and the optical axis 542 is inserted into the linear bearing 543, which reduces the friction during guiding and improves the guiding reliability.
  • two cover guide assemblies 54 are arranged at intervals along the Y direction, and the two cover guide assemblies 54 are respectively disposed on both sides of the cover drive assembly 52 .
  • the cover plate assembly 5 further includes a displacement detection assembly 20 .
  • the cover plate displacement detection assembly 20 includes a photoelectric sensor sheet 202 arranged on the horizontal connecting plate 513 and a photoelectric switch 201 arranged on the mounting plate 531 .
  • other detection devices may also be used to detect the running stroke of the capping assembly 51 , which will not be repeated here.
  • the top cover plate 511 , the vertical connecting plate 512 , the horizontal connecting plate 513 and the mounting plate 531 are respectively provided with weight reducing holes.
  • FIG. 13 is a schematic structural diagram of the magnetic attraction assembly provided by the embodiment of the application from one perspective
  • FIG. 14 is a schematic structural diagram of the magnetic attraction assembly provided by the embodiment of the application from another perspective
  • FIG. 15 is provided by the embodiment of the application.
  • a schematic diagram of the disassembled structure of the magnetic attraction assembly, Fig. 16 is a partial enlarged view of J in Fig. 15, as described in Fig. 13-Fig. 16, the magnetic attraction assembly 6 includes a magnetic rod assembly 61 and a driving magnetic rod assembly 61 along the direction toward or away from the reagent
  • the magnet bar drive assembly 62 for movement of the plate 4 in the direction.
  • the magnet bar assembly 61 is located below the reagent plate 4 , and the magnet bar driving assembly 62 is configured to drive the magnet bar assembly 61 to rise and fall vertically.
  • This arrangement can improve the compactness of the structure, and it is convenient for the magnetic attraction component 6 to perform magnetic attraction and expulsion reactions on the magnetic beads in the mixed solution in the reaction tube 43 .
  • the magnetic rod assembly 61 can also move toward or away from the reaction tube 43 in other moving directions, such as horizontal running or tilting movement.
  • the magnet bar assembly 61 includes a magnet bar bracket and a magnet bar 611, and the magnet bar 611 is vertically and detachably arranged on the magnet bar bracket.
  • the magnetic bar 611 By arranging the magnetic bar 611 , the magnetic bar 611 can be inserted between the reaction tube 43 on the reagent plate 4 and the adjacent tube structures, thereby reducing the interference between the magnetic attraction component 6 , the oscillating heating component 3 and the reagent plate 4 .
  • a plurality of magnet bars 611 are arranged side by side along the Y direction, and the number and position of the magnet bars 611 correspond to the number of the reagent plates 4 and the setting positions of the reaction tubes 43 one-to-one, and when the magnet bars 611 When the magnet bar 611 is located at the position where the magnetic attraction reaction is performed, the opening position of the magnet bar 611 and the opening of the tube insertion hole 319 on the reaction tube heating block 312 are directly opposite.
  • the magnet bar support includes a mounting bar 612 disposed along the Y direction, a horizontal mounting plate 613 , which is detachably connected to the mounting bar 612 , and the mounting cross plate 613 is connected to the magnet bar driving assembly 62 .
  • the mounting bar 612 has a positioning portion 6123 protruding downward, and a positioning groove 6131 is correspondingly formed on the upper surface of the mounting cross plate 613.
  • the positioning portion 6123 is inserted into the positioning groove 6131 to realize the installation and installation The installation positioning of the strip 612.
  • the mounting bar 612 and the mounting transverse plate 613 are detachably connected through the bottom of the positioning groove 6131 and the screws on the mounting portion.
  • the mounting bar 612 has a mounting hole 6121 extending vertically through the mounting bar 612 .
  • the magnet bar 611 is provided with a through hole 6122 in the Z direction. One end communicates with the mounting hole 6121 , and the opening width of the through hole 6122 is smaller than the diameter of the mounting hole 6121 .
  • the arrangement of the through-hole 6122 enables the mounting bar 612 to form a mounting arm structure with one end free.
  • the through-hole 6122 is opened under the action of external force, and closed when the external force is removed, so that the magnet bar 611
  • the mounting hole 6121 can be accessed through the through-hole 6122 .
  • the mounting bar 612 is made of elastic materials such as rubber and plastic.
  • the magnetic rod driving assembly 62 adopts a driving method in which a motor cooperates with a screw rod.
  • the magnetic rod driving assembly 62 includes a magnetic driving motor, a magnetic screw rod arranged along the Z direction and connected to the output shaft of the magnetic driving motor at its lower end, and a magnetic screw rod sleeved on the magnetic suction rod and horizontal to the installation.
  • the magnetic nut seat connected to the plate 613.
  • other structures of the magnetic drive assembly capable of vertically lifting and lowering the magnetic rod assembly 61 may also be used, such as using a linear motor, a hydraulic cylinder drive, etc., which will not be repeated here.
  • the magnetic attraction assembly 6 further includes a magnetic attraction guide assembly 63 arranged to be guided in the vertical direction.
  • the magnetic assembly 6 further includes a magnetic motor base 64 , and the fixed ends of the magnetic guide assembly 63 and the magnetic drive motor are arranged on the magnetic motor base 64 .
  • the arrangement of the magnetic motor base 64 can refer to the above-mentioned arrangement of the cover motor base 53
  • the magnetic guide assembly 63 can refer to the structural arrangement of the cover guide assembly 54 , which will not be repeated here.
  • two sets of magnetic guide assemblies 63 are arranged at intervals along the Y direction, and the magnetic drive assemblies are located between the two sets of magnetic guide assemblies 63 .
  • the magnetic attraction assembly 6 in order to detect the running displacement of the magnet rod assembly 61 , the magnetic attraction assembly 6 is provided with a displacement detection assembly 20 .
  • the displacement detection assembly 20 includes a photoelectric induction sheet 202 arranged on the horizontal mounting plate 613 and a photoelectric switch 201 arranged on the magnetic motor base 64 .
  • the first pipetting mechanism 7 is arranged at one end along the length direction of the mounting frame 2 , and the bottom plate 21 is provided with a reagent bottle storage area at one side along the length direction of the reagent plate 4 , and the reagent bottle storage area is provided with a reagent bottle storage area.
  • the pipetting device includes a first pipetting mechanism 7 and a second pipetting mechanism 8.
  • the first pipetting mechanism 7 is configured to absorb the liquid in the reagent bottle 40, and the second pipetting mechanism 8 is configured to pick and place the reagent plate 4. reagent.
  • the first pipetting mechanism 7 is arranged above the reagent storage area, and is configured to extract the liquid in the reagent bottle 40; the second pipetting mechanism 8 is configured to suck and transfer the liquid on the reagent plate 4.
  • each row of reagent bottles 40 includes five reagent bottles 40 arranged side by side along the X direction.
  • Different types of liquids can be stored in 40.
  • the number of columns of reagent bottles 40 in the reagent bottle storage area and the number of reagent bottles 40 in each column may be specifically determined according to the type of nucleic acid detection items.
  • FIG. 17 is a schematic structural diagram of the first pipetting mechanism 7 provided by the embodiment of the application from one perspective
  • FIG. 18 is a schematic structural diagram of the first pipetting mechanism 7 provided by the embodiment of the application from another perspective
  • FIG. 19 is A schematic diagram of the split structure of the first pipetting mechanism 7 provided in the embodiment of the present application, as shown in FIGS. 17-19
  • the first pipetting mechanism 7 includes a first reagent needle unit 71 , which is connected to the first reagent needle unit 71 and A first vertical displacement unit 73 configured to drive the first reagent needle unit 71 to rise and fall vertically and a first horizontal displacement unit connected to the first vertical displacement unit 73 and configured to drive the first reagent needle unit 71 to move in the horizontal direction 72.
  • the first horizontal displacement unit 72 includes a connecting piece 722 and a first horizontal driving component 721.
  • the connecting piece 722 is connected with the beam 221 of the mounting frame 2, and the fixed end of the first horizontal driving component 721 is set on the connecting piece 722.
  • the driving end of the assembly 721 is connected with the first vertical displacement unit 73 .
  • the first horizontal drive assembly 721 adopts a driving method in which a motor cooperates with a screw rod.
  • the first horizontal drive assembly 721 includes a horizontal drive motor, a horizontal screw rod arranged in a horizontal direction and one end of which is connected to the output shaft of the horizontal drive motor. , a horizontal nut seat sleeved on the horizontal screw rod, and the horizontal nut seat is connected with the vertical drive assembly.
  • the horizontal drive motor is fixed on the plate-shaped connecting member 722 through the motor fixing plate 723 .
  • the horizontal screw rod is set along the Y direction to drive the first reagent needle unit 71 to move along the Y direction.
  • the horizontal screw rod can also be set along the X direction to drive the first reagent needle unit 71 71 moves in the X direction.
  • the first vertical displacement unit 73 includes an adapter plate 731 arranged horizontally along the X direction and a first vertical drive assembly 732 disposed on the adapter plate 731 , and the first vertical drive assembly 732 includes a first vertical drive assembly 732 fixed on the adapter plate 731 A driving motor, a screw rod arranged vertically and one end connected to the driving motor, a nut seat sleeved on the screw rod, and the nut seat is connected with the first reagent needle unit 71 .
  • the first pipetting mechanism 7 further includes a first horizontal guide assembly 74 and a first vertical guide assembly 75, and the first horizontal guide assembly 74 is provided with On the connecting piece 722, the first vertical guide assembly 75 is arranged on the guide rail plate 77 which is connected with the adapter plate 731 and is arranged vertically.
  • the first horizontal guide assembly 74 and the first vertical guide assembly 75 are in the form of guide rail sliders, and the configuration of using the guide rail slider structure to achieve linear guidance is a conventional configuration in the field, and will not be repeated here.
  • both ends of each guide rail are connected with buffer blocks 76 .
  • the first reagent needle unit 71 includes a first reagent needle holder 712 arranged horizontally and a first reagent needle 711 arranged on the first reagent needle holder 712 .
  • the first reagent needle rack 712 is disposed along the X direction and one end is connected to the slider of the first vertical guide assembly 75 .
  • the first reagent needles 711 are arranged vertically, and a plurality of first reagent needles 711 are arranged at intervals along the X direction.
  • the number and position of the first reagent needles 711 correspond to the number and position of the reagent bottles 40 in each row of reagent bottles in the reagent bottle storage area on a one-to-one basis.
  • the first reagent needle 711 is plugged into the first reagent needle rack 712 , and the connection between the first reagent needle 711 and the first reagent needle rack 712 can also refer to the above-mentioned installation of the magnet bar 611 and the mounting bar 612 method, which will not be repeated here.
  • the first pipetting mechanism 7 in order to detect the displacement of the first horizontal displacement unit 72 and the first vertical displacement unit 73, the first pipetting mechanism 7 further includes a first horizontal displacement detection component and a first displacement detection component, and the first horizontal displacement
  • the displacement detection component and the first vertical displacement detection component adopt the detection form in which the photoelectric switch 201 cooperates with the photoelectric sensor sheet 202 , and details are not described herein again.
  • FIG. 20 is a schematic structural diagram of the second liquid pipetting mechanism provided by the embodiment of the application from one perspective
  • FIG. 21 is a schematic structural diagram of the second liquid pipetting mechanism provided by the application from another perspective
  • FIG. 22 is an embodiment of the application. 20-22
  • the second pipetting mechanism 8 includes a second reagent needle unit 81 configured to pipette liquid, and a second reagent needle unit 81 configured to drive the liquid.
  • the second vertical displacement unit 82 vertically lifted, the tip pick-and-place unit 83 configured to replace and insert the tip, the third vertical displacement unit 84 configured to drive the vertical lift of the tip pick-and-place unit 83, and the A second horizontal moving unit for driving the second vertical displacement unit 82 and the third vertical displacement unit 84 to move in the Y direction.
  • This arrangement can improve the compactness of the structure of the second pipetting mechanism 8 .
  • the second horizontal moving unit includes a fixed vertical plate 85 parallel to the XZ plane and a second horizontal driving unit (not shown).
  • the fixed end of the second horizontal driving unit is connected to the beam 221 , and the driving end of the second horizontal driving unit is connected to the fixed
  • the vertical plates 85 are connected and arranged to drive the connecting vertical plates to reciprocate along the Y direction.
  • the second horizontal driving unit can be in the form of a motor matching a screw nut, or a motor matching a sprocket chain, or other structures that can realize the horizontal movement of the fixed vertical plate 85 , which will not be repeated here. .
  • the second vertical displacement unit 82 and the third vertical displacement unit 84 are arranged on the same surface of the fixed vertical plate 85 .
  • the fixed vertical plate 85 is connected with a horizontal fixed horizontal plate 86 perpendicular to the fixed vertical plate 85 , and the fixed ends of the second vertical displacement unit 82 and the third vertical displacement unit 84 are connected to the fixed horizontal plate.
  • 86, and the second vertical displacement unit 82 and the third vertical displacement unit 84 are arranged side by side along the X direction.
  • the second vertical displacement unit 82 and the third vertical displacement unit 84 are driven by a motor and a screw nut, and have a simple structure and convenient connection.
  • the second vertical displacement unit 82 and the third vertical displacement unit 84 may also adopt a structural form in which a motor cooperates with a rack and pinion, or a driving form such as a linear motor, a hydraulic cylinder, or the like.
  • a driving form such as a linear motor, a hydraulic cylinder, or the like.
  • the structural form that can realize the linear drive in the vertical direction is a conventional setting in the field, and will not be repeated here.
  • the tip pick-and-place unit 83 includes a tip seat 831 , a tip cover rod 832 vertically arranged on the tip cover 831 , a tip needle 833 passing through the inside of the tip cover rod 832 , and a suction tip connected to the lower end of the tip cover rod 832 .
  • the tip nozzle 834, the lower end of the tip needle 833 is sealed and inserted into the tip nozzle 834.
  • the suction head seat 831 is detachably connected with the corresponding nut seat.
  • the suction head cover rod 832 is a hollow rod-shaped structure made of stainless steel, and the upper end of the suction head nozzle 834 is inserted into the lower end of the suction head cover rod 832 by interference.
  • the upper end of the tip needle 833 protrudes from the outside of the tip cover rod 832 to facilitate connection with the liquid inlet tube 702 .
  • the installation method of the suction head seat 831 and the suction head sleeve rod 832 can refer to the above-mentioned installation method of the magnetic rod 611 and the installation strip 612 , which will not be repeated here.
  • a plurality of suction head cover rods 832 are provided at intervals along the Y direction, so as to realize suction and replacement of multiple suction heads.
  • the number of the tip cover rods 832 is the same as the number of nucleic acid detections that the nucleic acid detector can perform synchronously at one time, and may be, but is not limited to, four.
  • the tip nozzle 834 is configured to insert and remove the tip located in the tip chamber 414 on the reagent plate 4.
  • the second pipetting mechanism 8 also includes a tip ejection set for ejecting the tip. plate 87.
  • the suction head plate 87 is detachably connected to the fixed vertical plate 85 , and the suction head plate 87 is provided with a suction head hole 871 .
  • the lower end of the tip cover rod 832 is inserted into the tip ejection hole 871 , and the inner diameter of the tip ejection hole 871 is larger than the maximum outer diameter of the tip nozzle 834 and smaller than the maximum outer diameter of the tip.
  • the second reagent needle unit 81 includes a needle holder 811, a waste needle 812 and a reagent needle bundle 813.
  • the needle holder 811 is detachably connected to the corresponding nut seat, and the waste needle 812 and the reagent needle bundle 813 are detachably arranged on the needle holder. 811 on.
  • the waste liquid needle 812 and the reagent needle bundle 813 are arranged side by side along the X direction and arranged at intervals, and the waste liquid is discharged by designing a dedicated waste liquid needle 812, which can reduce the contamination between the reagents.
  • a plurality of waste liquid needles 812 and reagent needle bundles 813 are arranged parallel to and spaced in the Y direction, and the number of waste liquid needles 812 and reagent needle bundles 813 is the same as the nucleic acid that the nucleic acid detector can perform at the same time at a time. The number of detections is the same.
  • the needle holder 811 includes a needle holder 8111, a support rod 8112 and a needle holder 8113.
  • the needle holder 8111 is connected to the corresponding nut holder, the upper end of the support rod 8112 is detachably connected to the needle holder 8111, and the support rod The lower end of 8112 is detachably connected to the needle mount 8113.
  • This setting method can reduce the overall weight of the needle holder 811, ensure the stability of the needle holder 811, and shorten the required stroke of the reagent needle bundle 813 and the waste needle 812 to be vertically lifted.
  • the reagent needle bundle 813 includes a needle bundle cannula 8132 and a plurality of second reagent needles 8131 passing through the needle bundle cannula 8132 , the needle bundle cannula 8132 , the waste liquid needle 812 and the needle mounting seat 8113
  • the connection method please refer to the above-mentioned installation method of the magnet bar 611 and the mounting bar 612, which will not be repeated here.
  • there are five second reagent needles 8131 in the needle bundle cannula 8132 and the number of the second reagent needles 8131 in each needle bundle cannula 8132 is the same as the number of the second reagent needles 8131 in the first pipetting mechanism 7 .
  • the number of reagent needles 711 is the same.
  • the second reagent needle 8131 includes a vertically arranged main body portion 81311 and a guide connected to the main body portion 81311 and forming a non-zero included angle with the main body portion 81311 part 81312, the main body part 81311 and the guide part 81312 are integrally formed, and the guide part 81312 is inclined outward relative to the central axis of the main body part 81311.
  • the included angle between the guide part 81312 and the main body part 81311 is 5°-10° °.
  • the inclination directions of the ends of the second reagent needles 8131 in the same reagent needle bundle 813 are different.
  • the second pipetting mechanism 8 further includes a second vertical guide assembly 88 and a third vertical guide assembly 89.
  • the second vertical guide The assembly 88 and the third vertical guide assembly 89 are arranged side by side along the Y direction, and the second vertical guide assembly 88 and the third vertical guide assembly 89 are in the form of guide rail sliders, and the suction head seat 831 and the needle fixing seat 8111 are respectively Connect with the slider of the corresponding vertical guide assembly.
  • the second pipetting mechanism 8 further includes a device configured to detect the vertical displacement of the second reagent needle unit 81 and the displacement of the tip pick-up unit 81, respectively.
  • the displacement detection assembly 20 for the vertical lifting displacement of the placing assembly adopts a structure in which the photoelectric switch 201 cooperates with the photoelectric induction sheet 202. In other embodiments, other detection devices capable of realizing displacement detection may also be used.
  • the fixed vertical plate 85 includes a detachable gantry portion 851 and a vertical plate portion 852.
  • the gantry portion 851 has a U-shaped structure with an open lower end. The two sides are detachably connected, the fixed horizontal plate 86 is arranged on the gantry portion 851 , and the two vertical guide assemblies are both arranged on the vertical plate portion 852 .
  • the pipetting mechanism further includes a pipeline assembly 70 and a pump unit.
  • the pipeline assembly 70 constitutes a pipeline required for pipetting, and the pump assembly provides power for picking and placing the liquid.
  • the pipeline assembly 70 includes a pipette pipe assembly and a reagent needle pipe assembly
  • the pump unit includes a syringe pump 10 and a liquid pump group 9
  • the syringe pump 10 communicates with the pipette tip pick-and-place unit 83 through the pipette pipe assembly Connection
  • the liquid pump group 9 is connected to the first reagent needle unit 71 and the second reagent needle unit 81 through the reagent needle pipeline assembly.
  • FIG. 23 is a schematic diagram of the pipeline connections of the suction head pipeline assembly, the syringe pump 10 and the suction head pick-and-place unit 83 provided by the embodiment of the application.
  • the suction head pipeline assembly includes a plurality of suction pipes 701 , the number of suction pipes 701 is the same as the number of suction needles 833 , one end of the suction pipe 701 is in sealing communication with the upper end of the suction needle 833 , and the other end of the suction pipe 701 is connected to the syringe pump 10 .
  • the arrangement of the pipette pipe assembly and the syringe pump 10 enables pipetting, pipetting and mixing of the reaction solution during the nucleic acid extraction process.
  • FIG. 24 is a schematic diagram of the connection relationship between the liquid pump and the reagent needle pipeline assembly 70 according to the embodiment of the application.
  • the liquid pump set 9 has multiple pairs of reagent liquid inlets and reagent liquid outlets
  • the reagent needle pipeline includes a The liquid inlet pipe 702 between the liquid pump group 9 and the first reagent needle 711, the first end of the liquid inlet pipe 702 is in sealing communication with the upper end of the first reagent needle 711, and the second end of the liquid inlet pipe 702 is connected to the liquid pump group 9. connected to the reagent inlet.
  • the reagent needle pipeline assembly also includes a main liquid outlet pipe 703.
  • the first end of the main liquid outlet pipe 703 is connected to the reagent liquid outlet corresponding to the above-mentioned liquid inlet pipe 702, and the second end of each main liquid outlet pipe 703 is connected to many
  • the first ends of the aliquots 704 and the second ends of the plurality of aliquots 704 are respectively connected to one of the second reagent needles 8131 in the plurality of reagent needle bundles 813 .
  • the reagent needle bundle 813 has four groups, and each main liquid outlet pipe 703 is connected with four branch liquid pipes 704 .
  • the reagent needle pipeline assembly also includes a main waste liquid inlet pipe 706 and a plurality of waste liquid inlet pipes 707, the number of the waste liquid inlet pipes 707 is the same as the number of waste liquid needles 812, and each waste liquid inlet pipe 707
  • the first end of the waste liquid is connected to the upper end of a waste liquid needle 812
  • the second end of the waste liquid inlet pipe 707 is connected to the first end of the main waste liquid inlet pipe 706, and the second end of the main waste liquid inlet pipe 706 is connected to the liquid pump group 9
  • the waste liquid inlet port in , the waste liquid outlet port corresponding to the waste liquid inlet port is connected to the first end of the waste liquid outlet pipe 707
  • the second end of the waste liquid outlet pipe 707 is connected to the waste liquid bottle 80 .
  • the waste liquid bottle 80 is made of a plastic material, and can contain a disinfectant such as sodium chlorate, which is used for the discharge of waste liquid and the digestion of nucleic acid products.
  • the reagent bottle storage area is further provided with a The liquid level sensing assembly 30 is configured to detect the liquid level in each reagent bottle 40 .
  • the liquid level sensing assembly 30 is configured to detect the liquid level in each reagent bottle 40 .
  • the reagent bottle 40 is disposed on the liquid level sensing assembly 30 , and the liquid level sensing assembly 30 is provided with a heating block and a radiator corresponding to each reagent bottle 40 to heat or dissipate the reagent in the reagent bottle 40 .
  • the casing 1 in order to ensure the heat dissipation and ventilation inside the nucleic acid detector, the casing 1 is provided with an air extraction filter system, which can promote the air flow in the accommodation space, drive the heat to be dissipated in time and effectively, and can carry out Filtration can reduce the contamination of reagents and samples on the reagent plate 4, and can also prevent cross-contamination between samples and reagents in the nucleic acid detector.
  • the ventilation filtration system has a nucleic acid filtering function to prevent pollution to the external environment.
  • the settings of the ventilation filtration system can refer to the settings of the existing ventilation filtration system, which will not be repeated here.
  • a sterilization and disinfection system is also provided inside the casing 1, and the sterilization and disinfection system sterilizes and sterilizes the equipment inside the casing 1, further reducing nucleic acid pollution and reducing the impact on the staff.
  • the sterilization and disinfection system adopts ultraviolet sterilization, but it can be understood that other methods capable of sterilization and disinfection may also be used.
  • the nucleic acid detector integrateds nucleic acid extraction and purification, nucleic acid amplification and molecular hybridization detection of biological samples on one instrument.
  • the operator only needs to perform simple preparations to complete all detection within 3.5h-6h
  • the result is obtained, that is, the operator only needs to put the disposable reagent plate 4, put the amplification tube, put the hybridization reagent, add the sample and start the instrument.
  • the nucleic acid detector has a large processing capacity and can process 24 samples at a time. The whole process is efficient and convenient, and is suitable for clinical testing.
  • This embodiment provides a nucleic acid detection method, which is applied to the nucleic acid detector in the first embodiment, and is used for efficient and automated nucleic acid detection items.
  • Nucleic acid detection methods include five majors: preliminary preparation, nucleic acid extraction and purification, nucleic acid amplification reaction and nucleic acid hybridization reaction.
  • the preliminary preparations include:
  • the nucleic acid detection item to be performed is selected and activated through the display screen 60.
  • S101-S104 are operations performed manually.
  • the cover plate assembly 5 moves downward to close the nozzle of the nucleic acid amplification tube 42.
  • the liquid pump group 9 draws the hybridization liquid through the first reagent needle 711 to fill the pipeline.
  • S105-S107 it is the automatic operation of the nucleic acid detector, and S105, S106 and S107 can be performed synchronously, without distinction.
  • Magnetic bead-based nucleic acid extraction and purification including:
  • the second pipetting mechanism 8 moves, so that the tip nozzle 834 runs to the tip chamber 414 to insert the tip.
  • the second pipetting mechanism 8 moves, and the sample liquid is taken from the sample tube 44 and added to the reagent tube 42 containing proteinase K and magnetic beads.
  • the syringe pump 10 is actuated, so that the suction head repeatedly sucks and releases the liquid in the reagent tube 42, so as to blow the liquid at least once, so as to disperse the magnetic beads in the reagent tube 42.
  • the second pipetting mechanism 8 is actuated, and the pipette tip extracts the mixed solution of proteinase K, magnetic beads and sample into the reaction tube 43 containing the magnetic bead binding solution.
  • the heating film 315 corresponding to the reaction tube heating block 312 is energized and operated to heat the reaction tube 43.
  • the syringe pump 10 operates to repeatedly blow the liquid in the reaction tube 43 at least once; during this process, the sample releases nucleic acid As well as impurities such as proteins, nucleic acids are adsorbed on the magnetic beads.
  • the magnetic attraction component 6 is actuated, so that the magnetic rod 611 is moved up and moved to the middle of one side of the reaction tube 43 to attract magnetism; the nucleic acid is concentrated in one area through S206.
  • the second pipetting mechanism 8 and the syringe pump 10 act to suck the liquid in the reaction tube 43 into the waste liquid tank 415; since the nucleic acid has been concentrated in one area, the nucleic acid can be avoided when the mixed liquid is sucked. Thereby, the nucleic acid can be preserved to the maximum extent and the accuracy of nucleic acid concentration measurement can be improved.
  • the second pipetting mechanism 8 and the syringe pump 10 act to suck the liquid from the reagent tube 42 containing the washing solution into the reaction tube 43, and the magnetic suction assembly 6 acts to move the magnetic rod 611 downward to away from the reaction tube 43.
  • the syringe pump 10 is actuated, and the liquid in the reaction tube 43 is repeatedly sucked and released by the suction head to realize repeated blowing and blowing of the liquid in the reaction tube 43; the nucleic acid on the magnetic beads is washed in S209 to improve the purity of the nucleic acid .
  • the magnetic attraction component 6 is actuated, so that the magnetic rod 611 is moved up and moved to the middle of one side of the reaction tube 43 to attract magnetism.
  • the magnetic attraction assembly 6 is actuated to move the magnetic rod 611 downward to away from the reaction tube 43, the heating film 315 corresponding to the reaction tube heating block 312 is energized and heated, and the reaction tube 43 is heated to evaporate the residual liquid.
  • the second pipetting mechanism 8 operates to discard the tips on the tip nozzle 834 into the tip chamber 414 .
  • the second pipetting mechanism 8 is actuated, the suction head sucks the eluent into the reaction tube 43, and repeatedly blows the liquid in the reaction tube 43 at least once.
  • the heating membrane 315 corresponding to the heating block 312 of the reaction tube is heated by electricity, so that the reaction tube 43 is heated to elute the nucleic acid.
  • the magnetic attraction assembly 6 is actuated, and the magnetic rod 611 is moved to one side of the reaction tube 43 to attract magnetism, and all the magnetic beads are collected on the upper part of the tube wall of the reaction tube 43 to keep the magnetic beads away from the bottom of the tube.
  • Nucleic acid amplification realizes the replication process of nucleic acid molecules based on PCR technology or constant temperature amplification technology, including:
  • the cover plate assembly 5 moves upward to open the opening of the nucleic acid amplification tube.
  • the second pipetting mechanism 8 is actuated, and the pipette tip takes the quantitative nucleic acid from the reaction tube 43 into the nucleic acid amplification tube, and pipetting is repeated. Discard the tips on the tip nozzle 834.
  • the cover plate assembly 5 moves downward to seal the mouth of the nucleic acid amplification tube.
  • the system starts the nucleic acid amplification program to perform the nucleic acid amplification reaction, during which the temperature conditions required for the nucleic acid amplification reaction are controlled by the heat treatment module.
  • the hybridization reaction realizes the hybridization recognition of molecules based on the principle of base complementarity, including:
  • the cover plate assembly 5 operates to open the opening of the nucleic acid amplification tube 42.
  • the second pipetting mechanism 8 is activated to insert a new pipette tip, the second pipetting mechanism 8 and the syringe pump 10 are activated, and the amplification solution in the nucleic acid amplification tube is sucked into the hybridization solution containing the A solution through the pipette head In tank 412, hybridization begins.
  • the oscillating heating element 3 is actuated to drive the hybridization tank 412 to oscillate back and forth along the X direction.
  • the first pipetting mechanism 7, the second pipetting mechanism 8 and the liquid pump group 9 are activated, and the liquid in the reagent bottle 40 containing the B hybridization liquid is sucked through the first reagent needle 711 and the second reagent needle 8131 to hybridization tank 412.
  • S405-S407 are repeatedly executed until the liquids in the reagent bottles 40 carrying the C hybridization solution, the D hybridization solution and the E hybridization solution in the reagent bottle storage area are sequentially extracted into the hybridization tank 412. During this process, The oscillating heating assembly 3 continues to perform the X-direction oscillating action.
  • the first pipetting mechanism 7 operates, and the purified water in the reagent bottle 40 containing the purified water is extracted through the first reagent needle 711, and the pipelines in the reagent tube 42-way assembly are cleaned with the purified water.

Abstract

The present application belongs to the technical field of biological detection, and particularly discloses a nucleic acid detector and a nucleic acid detection method. The nucleic acid detector comprises: a mounting rack; a reagent plate, provided with a nucleic acid amplification tube, a hybridization tank, a reaction tube and reagent tubes for storing and extracting the reagent; a cover plate assembly, capable of moving in a direction toward or away from the nucleic acid amplification tube, so as to close or open the tube opening of the nucleic acid amplification tube; a heat treatment unit, used for heating or cooling the liquid on the reagent plate; a pipetting device, used for aspirating and dispensing the liquid on the reagent plate; an oscillating unit, used for driving the reagent plate to oscillate reciprocally in the horizontal direction; a magnetic attraction assembly, used for attracting and releasing magnetic beads in the reaction liquid on the reagent plate. The nucleic acid detection method uses the described nucleic acid detector to perform nucleic acid detection. The present nucleic acid detector and nucleic acid detection method can improve the efficiency of nucleic acid detection.

Description

核酸检测仪及核酸检测方法Nucleic acid detector and nucleic acid detection method
本申请要求申请日为2020年10月27日、申请号为202011165266.3的中国专利申请的优先权，该申请的全部内容通过引用结合在本申请中。This application claims the priority of the Chinese patent application with an application date of October 27, 2020 and an application number of 202011165266.3, the entire contents of which are incorporated herein by reference.
技术领域technical field
本申请涉及生物检测技术领域，例如涉及一种核酸检测仪及核酸检测方法。The present application relates to the technical field of biological detection, for example, to a nucleic acid detector and a nucleic acid detection method.
背景技术Background technique
核酸杂交技术是将特异的探针分别固定到固相材料上，再将经核酸扩增反应产生的特异性扩增的产物与之杂交，这样待检样本就会与具有同源序列的探针结合，由于待测样本具有生物素类或者荧光素或者地高辛等标记物，结合了待测样本的探针点上就带有生物素类或者荧光素或者地高辛等的标记物，再经相应的化学发光反应或者荧光激发就能显出杂交信号。这种检测反应一次杂交反应可以检测多种靶序列，具有快速简便、敏感度高和特异性强的特点，尤其是在基因分型、基因突变检测、病原体的检测等方面有独特的优势。Nucleic acid hybridization technology is to immobilize specific probes on solid-phase materials respectively, and then hybridize the specific amplified product generated by nucleic acid amplification reaction with it, so that the sample to be tested will be with the probe with homologous sequence. Binding, because the sample to be tested has markers such as biotin, fluorescein, or digoxigenin, the probe points that bind to the sample to be tested have markers such as biotin, fluorescein, or digoxigenin, etc. The hybridization signal can be displayed by the corresponding chemiluminescence reaction or fluorescence excitation. This detection reaction can detect a variety of target sequences in one hybridization reaction, and has the characteristics of rapidity, simplicity, high sensitivity and strong specificity, especially in genotyping, gene mutation detection, pathogen detection, etc. It has unique advantages.
基于核酸杂交技术进行的生物样本分子诊断，从临床样本收集到通过杂交检测出结果，需要经过样本前处理、样本核酸提取和纯化、核酸扩增和分子杂交等。整个实验过程包含样本核酸获取、样本核酸扩增复制、样本核酸检测分析等大体三个，这三个过程繁琐、试验时间长，对操作人员技术要求较高，对实验设备设施也存在较高要求；同时实验过程中存在样本交叉污染、试剂交叉污染和扩增产物交叉污染等可能，生物样本还存在对实验操作人员的可能的生物安全危害，以上种种因素极大地限制了分子杂交技术在核酸检测方面的应用。Molecular diagnosis of biological samples based on nucleic acid hybridization technology, from the collection of clinical samples to the detection of results by hybridization, requires sample pretreatment, sample nucleic acid extraction and purification, nucleic acid amplification and molecular hybridization. The entire experimental process includes three parts: sample nucleic acid acquisition, sample nucleic acid amplification and replication, and sample nucleic acid detection and analysis. These three processes are cumbersome and take a long time to test, which requires high technical requirements for operators and high requirements for experimental equipment and facilities. At the same time, there are possibilities of cross-contamination of samples, cross-contamination of reagents and cross-contamination of amplification products during the experiment, and biological samples also have possible biological safety hazards to experimental operators. The above factors greatly limit the application of molecular hybridization technology in nucleic acid detection. aspect applications.
相关技术中，基于传统解决方案，商业应用于诊断的核酸提取和纯化、核酸扩增、核酸分子杂交等仪器设备都是分立的，如提取核酸用的样本制备仪、核酸提取仪；扩增用的PCR仪或者其他核酸扩增仪等；杂交用的自动化杂交仪等。完成整个实验的具体过程包括但不限于将样本手工加入全自动核酸提取仪或者全手工提取，利用全自动核酸提取仪或者全手工对生物样本进行提取纯化后，人工把纯化好的核酸溶液转移至核酸扩增仪器，利用核酸扩增仪器对提取的核酸进行扩增，然后将扩增产物加入全自动杂交仪进行最后的杂交分析，非肉眼可见的化学发光方法还需要配备荧光扫描仪等。整个实验要么手工进行或者衔 接，要么提取与扩增、分子杂交等分别由不同的仪器来实现。由此导致了以下问题：In related technologies, based on traditional solutions, instruments and equipment for nucleic acid extraction and purification, nucleic acid amplification, and nucleic acid molecular hybridization that are commercially used in diagnosis are all discrete, such as sample preparation instruments and nucleic acid extraction instruments for nucleic acid extraction; PCR instrument or other nucleic acid amplification instrument, etc.; automated hybridization instrument for hybridization, etc. The specific process of completing the entire experiment includes, but is not limited to, manually adding the sample to the automatic nucleic acid extractor or fully manual extraction, using the automatic nucleic acid extractor or fully manual extraction and purification of the biological sample, and manually transferring the purified nucleic acid solution to the laboratory. Nucleic acid amplification instruments use nucleic acid amplification instruments to amplify the extracted nucleic acids, and then add the amplified products to an automatic hybridization instrument for final hybridization analysis. Non-visual chemiluminescence methods also need to be equipped with a fluorescence scanner, etc. The whole experiment is either performed manually or concatenated, or the extraction and amplification, molecular hybridization, etc. are performed by different instruments respectively. This leads to the following problems:
1.整个核酸检测过程需要素质极高的专业实验人员全程跟进，增加了人力成本和人为可能出现差错的几率；同时在处理可能的中高传染性样本时，对实验人员的生命安全和整个实验环境造成生物安全威胁。1. The entire nucleic acid detection process requires highly qualified professional laboratory personnel to follow up the whole process, which increases labor costs and the possibility of human errors; at the same time, when processing possible medium and high infectious samples, it is important to the life safety of the laboratory personnel and the entire experiment. The environment poses a biosecurity threat.
2.整个核酸检测过程分阶段进行，需要人工的介入，增加了试验的不可控性和不确定性，同时试验时间漫长，检测效率低下或者被制约。2. The entire nucleic acid detection process is carried out in stages, requiring manual intervention, which increases the uncontrollability and uncertainty of the test. At the same time, the test time is long, and the detection efficiency is low or restricted.
3.整个核酸检测过程需要中高速离心机、水浴锅、移液器、核酸提取和纯化仪、试剂分装仪、核酸扩增仪、杂交仪、扫描仪等各种仪器设备10种以上、多种配套耗材等多达15种以上；同时为了满足检测要求，还需要配备至少30种不同溶液等，整个试剂耗材仪器的配备和制备极为复杂。3. The entire nucleic acid detection process requires more than 10 kinds of equipment such as medium and high-speed centrifuges, water baths, pipettes, nucleic acid extraction and purification instruments, reagent dispensers, nucleic acid amplification instruments, hybridization instruments, and scanners. There are more than 15 kinds of supporting consumables, etc. At the same time, in order to meet the detection requirements, at least 30 different solutions are required, and the equipment and preparation of the entire reagent consumables and instruments are extremely complicated.
4.整个核酸检测过程需要人工转移样本、多种中间溶液等，操作环境相对开放，增加了样本间相互污染的危险、也增加了溶液相互污染的风险，从而导致整个核酸检测的实施对环境要求极高；同时配备的仪器耗材昂贵，不适合大面积推广应用。4. The entire nucleic acid detection process requires manual transfer of samples, various intermediate solutions, etc. The operating environment is relatively open, which increases the risk of mutual contamination between samples and the risk of mutual contamination of solutions, resulting in the implementation of the entire nucleic acid detection. Environmental requirements Extremely high; the instrument and consumables equipped at the same time are expensive, which is not suitable for large-scale promotion and application.
发明内容SUMMARY OF THE INVENTION
本申请提供了一种核酸检测仪，能够提高核酸检测效率，降低核酸检测成本。The present application provides a nucleic acid detector, which can improve nucleic acid detection efficiency and reduce nucleic acid detection costs.
本申请还提供了一种核酸检测方法，能够提高核酸检测效率，降低核酸检测成本。The present application also provides a nucleic acid detection method, which can improve the nucleic acid detection efficiency and reduce the nucleic acid detection cost.
一实施例提供了一种核酸检测仪，包括：安装架；试剂板；设置于所述试剂板上的核酸扩增管、杂交槽、反应管及存放提取试剂的试剂管；盖板组件，设置在所述安装架上，且能够沿朝向或远离所述核酸扩增管的方向运动，以封闭或开放所述核酸扩增管的管口；热处理单元，设置在所述安装架上，且设置为对所述试剂板上液体进行加热或冷却处理；移液装置，设置在所述安装架上，且设置为对所述试剂板上的液体进行吸取及转移；振荡单元，设置在所述安装架上且与试剂板相对固定，所述振荡单元设置为带动所述试剂板沿水平方向往复振荡；及磁吸组件，设置在所述安装架上，且能沿朝向或远离所述试剂板的方向运动，所述磁吸组件设置为对所述试剂板上的反应液进行吸磁和放磁。An embodiment provides a nucleic acid detector, including: a mounting frame; a reagent plate; nucleic acid amplification tubes, hybridization tanks, reaction tubes and reagent tubes for storing extraction reagents arranged on the reagent plate; a cover plate assembly, set on the mounting rack, and can move in the direction toward or away from the nucleic acid amplification tube to close or open the nozzle of the nucleic acid amplification tube; a heat treatment unit, arranged on the mounting rack, and arranged In order to heat or cool the liquid on the reagent plate; a pipetting device is arranged on the mounting frame, and is arranged to suck and transfer the liquid on the reagent plate; an oscillation unit is arranged on the installation frame The oscillating unit is arranged to drive the reagent plate to oscillate back and forth in a horizontal direction; and a magnetic attraction component is arranged on the mounting frame and can be moved toward or away from the reagent plate. moving in a direction, the magnetic attraction assembly is configured to magnetically attract and release the reaction liquid on the reagent plate.
一实施提供了一种核酸检测方法，应用于如上所述的核酸检测仪，且所述 核酸检测方法包括：检测前处理、核酸提取和纯化、核酸扩增及杂交反应。One implementation provides a nucleic acid detection method, which is applied to the nucleic acid detector as described above, and the nucleic acid detection method includes: pre-detection treatment, nucleic acid extraction and purification, nucleic acid amplification and hybridization reaction.
所述检测前处理包括：打开所述核酸扩增管的管盖；及所述盖板组件将所述核酸扩增管的管口封闭。The pre-detection treatment includes: opening the cap of the nucleic acid amplification tube; and closing the mouth of the nucleic acid amplification tube by the cover plate assembly.
基于磁珠法进行所述核酸提取和纯化，且所述核酸提取和纯化包括：所述移液装置将样本加入含有蛋白酶K和磁珠的所述试剂管中，混匀；所述移液装置吸取混匀后的蛋白酶K、磁珠和样本液体的混合液至含有磁珠结合液的反应管中；热处理单元调控所述反应管反应所需温度条件，以使细胞裂解，释放核酸；及在释放核酸后，通过所述磁吸组件沿靠近或远离所述试剂板的往复运动并配合所述移液装置的液体抽取和转移实现核酸在磁珠上吸附、洗涤和洗脱，以得到纯化的核酸。The nucleic acid extraction and purification is performed based on the magnetic bead method, and the nucleic acid extraction and purification includes: the pipetting device adds a sample to the reagent tube containing proteinase K and magnetic beads, and mixes it evenly; the pipetting device Pipetting the mixed solution of proteinase K, magnetic beads and sample liquid after mixing into a reaction tube containing the magnetic bead binding solution; the heat treatment unit regulates the temperature conditions required for the reaction of the reaction tube to lyse cells and release nucleic acids; and After the nucleic acid is released, the nucleic acid is adsorbed, washed and eluted on the magnetic beads through the reciprocating motion of the magnetic attraction component close to or away from the reagent plate and the liquid extraction and transfer of the pipetting device to obtain purified nucleic acid. nucleic acid.
所述核酸扩增反应包括：盖板组件运动，使核酸扩增管的管口开放；移液装置吸取一定量的核酸至核酸扩增管中；盖板组件运动，将所述核酸扩增管的管口封闭；及启动核酸扩增程序，进行核酸扩增反应，期间，通过所述热处理单元控制核酸扩增反应所需的温度条件。The nucleic acid amplification reaction includes: the movement of the cover plate assembly to open the mouth of the nucleic acid amplification tube; the pipetting device sucks a certain amount of nucleic acid into the nucleic acid amplification tube; the movement of the cover plate assembly makes the nucleic acid amplification tube open. and start the nucleic acid amplification procedure to carry out the nucleic acid amplification reaction, during which the temperature conditions required for the nucleic acid amplification reaction are controlled by the heat treatment unit.
所述杂交反应包括：盖板组件运动，将所述核酸扩增管的管口开放；所述移液装置抽取核酸扩增管的中的扩增溶液至杂交槽中；及移液装置依次抽取不同的杂交液至杂交槽中，热处理模块调控反应所需温度，所述振荡单元带动所述试剂板水平往复振荡，以实现扩增产物和杂交膜上探针的特异性结合、洗涤和显色。The hybridization reaction includes: moving the cover plate assembly to open the mouth of the nucleic acid amplification tube; the pipetting device extracting the amplification solution in the nucleic acid amplification tube into the hybridization tank; and the pipetting device sequentially extracting Different hybridization solutions are put into the hybridization tank, the temperature required for the reaction is regulated by the heat treatment module, and the oscillation unit drives the reagent plate to vibrate horizontally and reciprocally to realize the specific binding, washing and color development of the amplification product and the probe on the hybridization membrane. .
附图说明Description of drawings
图1为本申请实施例一提供的核酸检测仪的结构示意图；1 is a schematic structural diagram of the nucleic acid detector provided in Embodiment 1 of the present application;
图2为本申请实施例一提供的核酸检测仪的内部结构示意图；2 is a schematic diagram of the internal structure of the nucleic acid detector provided in Embodiment 1 of the present application;
图3为本申请实施例一提供的核酸检测仪的拆分结构示意图；3 is a schematic diagram of a disassembled structure of the nucleic acid detector provided in the first embodiment of the application;
图4为本申请实施例一提供的外壳的拆分结构示意图；4 is a schematic view of the split structure of the housing provided in Embodiment 1 of the present application;
图5为本申请实施例一提供的试剂板的结构示意图；5 is a schematic structural diagram of the reagent plate provided in Embodiment 1 of the present application;
图6是本申请实施例一提供的振荡加热组件与试剂板的结构示意图；6 is a schematic structural diagram of the oscillating heating assembly and the reagent plate provided in Embodiment 1 of the present application;
图7是本申请实施例一提供的振荡加热组件在一个视角下的结构示意图；7 is a schematic structural diagram of the oscillating heating assembly provided in Embodiment 1 of the present application from a viewing angle;
图8为7中I处的局部放大图；Fig. 8 is a partial enlarged view at I place in 7;
图9为本申请实施例一提供的振荡加热组件的拆分结构示意图；FIG. 9 is a schematic view of the disassembled structure of the oscillating heating assembly provided in the first embodiment of the application;
图10为本申请实施例一提供的盖板组件在一个视角下的结构示意图；10 is a schematic structural diagram of the cover plate assembly provided in Embodiment 1 of the present application from a viewing angle;
图11为本申请实施例一提供的盖板组件的另一个视角下的结构示意图；11 is a schematic structural diagram of the cover plate assembly provided in Embodiment 1 of the present application from another perspective;
图12为本申请实施例一提供的盖板组件的拆分结构示意图；FIG. 12 is a schematic view of the disassembled structure of the cover plate assembly provided in the first embodiment of the application;
图13为本申请实施例一提供的磁吸组件在一个视角下的结构示意图；13 is a schematic structural diagram of the magnetic attraction assembly provided in Embodiment 1 of the present application from a viewing angle;
图14为本申请实施例一提供的磁吸组件在另一个视角下的结构示意图；14 is a schematic structural diagram of the magnetic attraction assembly provided in Embodiment 1 of the present application from another perspective;
图15为本申请实施例一提供的磁吸组件的拆分结构示意图；15 is a schematic diagram of a disassembled structure of the magnetic attraction component provided in Embodiment 1 of the present application;
图16为图15中J处的局部放大图；Fig. 16 is a partial enlarged view at J in Fig. 15;
图17为本申请实施例一提供的第一移液机构在一个视角下的结构示意图；17 is a schematic structural diagram of the first liquid pipetting mechanism provided in Embodiment 1 of the present application from a viewing angle;
图18为本申请实施例一提供的第一移液机构在另一个视角下的结构示意图；18 is a schematic structural diagram of the first pipetting mechanism provided in Embodiment 1 of the present application from another perspective;
图19为本申请实施例一提供的第一移液机构的拆分结构示意图；19 is a schematic diagram of a disassembled structure of the first pipetting mechanism provided in Embodiment 1 of the application;
图20为本申请实施例一提供的第二移液机构在一个视角下的结构示意图；FIG. 20 is a schematic structural diagram of the second pipetting mechanism provided in Embodiment 1 of the application from one viewing angle;
图21为本申请实施例一提供的的第二移液机构在另一个视角下的结构示意图；21 is a schematic structural diagram of the second pipetting mechanism provided in Embodiment 1 of the application from another perspective;
图22为本申请实施例一提供的第二移液机构的拆分结构示意图；22 is a schematic diagram of a disassembled structure of the second pipetting mechanism provided in Embodiment 1 of the present application;
图23为本申请实施例一提供的吸头管路组件、注射泵及吸头取放单元的管路连接示意图；FIG. 23 is a schematic diagram of the pipeline connection of the suction pipe assembly, the syringe pump, and the suction head pick-and-place unit provided in the first embodiment of the application;
图24为本申请实施例一提供液泵及试剂针管路组件的连接关系示意图。FIG. 24 is a schematic diagram of the connection relationship between the liquid pump and the reagent needle pipeline assembly provided in the first embodiment of the application.
图中标记如下：The figures are marked as follows:
1、外壳；11、主壳体；12、第一前门；13、第二前门；131、安装口；14、后门；15、框架；16、废液收集槽；1. Shell; 11. Main shell; 12. First front door; 13. Second front door; 131. Installation port; 14. Back door; 15. Frame; 16. Waste liquid collection tank;
2、安装架；21、底板；22、支撑架；221、横梁；222、竖梁；23、支脚；2. Mounting frame; 21. Bottom plate; 22. Support frame; 221. Beam; 222. Vertical beam; 23. Support foot;
3、振荡加热组件；31、热处理单元；311、核酸扩增管加热块；312、反应管加热块；313、提取试剂加热块；314、杂交槽加热板；315、加热膜；316、散热单元；3161、散热器；31611、竖板部；31612、横板部；31613、翅片部；3162、散热风扇；317、制冷单元；319、管插孔；3110、减重槽；32、振荡单元；321、支撑板；322、X向导向组件；3221、X向导轨；3222、X向滑块；323、限位块；324、固定支架；33、隔热单元；331、隔热块；332、隔热柱；333、隔热载板；334、隔热支柱；335、隔热板；3. Oscillation heating assembly; 31. Heat treatment unit; 311, Nucleic acid amplification tube heating block; 312, Reaction tube heating block; 313, Extraction reagent heating block; 314, Hybridization tank heating plate; 315, Heating film; 316, Heat dissipation unit 3161, radiator; 31611, vertical plate part; 31612, horizontal plate part; 31613, fin part; 3162, cooling fan; 317, refrigeration unit; 321, support plate; 322, X-direction guide assembly; 3221, X-direction guide rail; 3222, X-direction slider; 323, limit block; 324, fixed bracket; 33, heat insulation unit; 331, heat insulation block; 332 , Insulation column; 333, Insulation carrier plate; 334, Insulation pillar; 335, Insulation board;
4、试剂板；41、承载主板；411、扩增孔位；412、杂交槽；413、卡口；414、吸头腔；415、废液槽；42、试剂管；43、反应管；44、样本管；45、吸头管；4. Reagent plate; 41. Main board; 411. Amplification well position; 412, Hybridization tank; 413, Bayonet; 414, Tip chamber; 415, Waste liquid tank; , sample tube; 45, pipette tip;
5、盖板组件；51、盖封组件；511、顶盖板；512、竖连接板；513、横连 接板；5131、第一横板部；5132、第二横板部；5133、光轴孔；514、盖封件；52、盖板驱动组件；521、盖板电机；522、盖板丝杆；523、盖板螺母座；53、盖板电机座；531、安装板；5311、穿板口；532、安装座；533、电机支板；54、盖板导向组件；541、光轴座；542、光轴；543、直线轴承；5, cover plate assembly; 51, cover seal assembly; 511, top cover plate; 512, vertical connecting plate; 513, horizontal connecting plate; 5131, first horizontal plate part; 5132, second horizontal plate part; 5133, optical axis hole; 514, cover seal; 52, cover drive assembly; 521, cover motor; 522, cover screw; 523, cover nut seat; 53, cover motor seat; 531, mounting plate; 5311, through plate mouth; 532, mounting seat; 533, motor support plate; 54, cover plate guide assembly; 541, optical axis seat; 542, optical axis; 543, linear bearing;
6、磁吸组件；61、磁棒组件；611、磁棒；612、安装条；6121、安装孔；6122、穿口；6123、定位部；613、安装横板；6131、定位槽；62、磁棒驱动组件；63、磁吸导向组件；64、磁吸电机座；6. Magnetic suction assembly; 61, Magnetic rod assembly; 611, Magnetic rod; 612, Mounting strip; 6121, Mounting hole; 6122, Piercing; 6123, Positioning part; 613, Mounting cross plate; 6131, Positioning slot; 62, Magnetic rod drive assembly; 63, magnetic guide assembly; 64, magnetic motor seat;
7、第一移液机构；71、第一试剂针单元；711、第一试剂针；712、第一试剂针架；72、第一水平位移单元；721、第一水平驱动组件；722、连接件；723、电机固定板；73、第一竖直位移单元；731、转接板；732、第一竖直驱动组件；74、第一水平导向组件；75、第一竖直导向组件；76、缓冲块；77、导轨板；7. The first pipetting mechanism; 71, the first reagent needle unit; 711, the first reagent needle; 712, the first reagent needle holder; 72, the first horizontal displacement unit; 721, the first horizontal drive assembly; 722, the connection 723, motor fixing plate; 73, first vertical displacement unit; 731, adapter plate; 732, first vertical drive assembly; 74, first horizontal guide assembly; 75, first vertical guide assembly; 76 , buffer block; 77, guide rail plate;
8、第二移液机构；81、第二试剂针单元；811、针架；8111、针固定座；8112、支撑杆；8113、针安装座；812、废液针；813、试剂针束；8131、第二试剂针；81311、主体部；81312、导向部；8132、针束套管；82、第二竖直位移单元；83、吸头取放单元；831、吸头座；832、吸头套杆；833、吸头针；834、吸头嘴；84、第三竖直位移单元；85、固定立板；851、门架部；852、立板部；86、固定横板；87、退吸头板；871、退吸头孔；88、第二竖直导向组件；89、第三竖直导向组件；810、缓冲块；8. The second pipetting mechanism; 81. The second reagent needle unit; 811, the needle holder; 8111, the needle holder; 8112, the support rod; 8113, the needle mount; 812, the waste liquid needle; 813, the reagent needle bundle; 8131, the second reagent needle; 81311, the main body part; 81312, the guide part; 8132, the needle bundle cannula; 82, the second vertical displacement unit; head cover rod; 833, tip needle; 834, tip nozzle; 84, third vertical displacement unit; 85, fixed vertical plate; 851, gantry part; 852, vertical plate part; 86, fixed horizontal plate; 87, suction head plate; 871, suction head hole; 88, second vertical guide assembly; 89, third vertical guide assembly; 810, buffer block;
9、液泵组；10、注射泵；9. Liquid pump group; 10. Syringe pump;
20、位移检测组件；201、光电开关；202、光电感应片；20, displacement detection component; 201, photoelectric switch; 202, photoelectric sensor sheet;
30、液位感应组件；40、试剂瓶；50、耗材面板；501、试剂板口；60、显示屏；70、管路组件；701、吹吸管；702、进液管；703、主出液管；704、分出液管；705、分废液进管；706、主废液进管；707、废液出管；80、废液瓶。30. Liquid level sensing assembly; 40. Reagent bottle; 50. Consumables panel; 501, Reagent plate mouth; 60, Display screen; 70, Pipeline assembly; 701, Blow pipe; pipe; 704, separate liquid pipe; 705, separate waste liquid inlet pipe; 706, main waste liquid inlet pipe; 707, waste liquid outlet pipe; 80, waste liquid bottle.
具体实施方式Detailed ways
在本申请的描述中，除非另有明确的规定和限定，术语“相连”、“连接”、“固定”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或成一体；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系。对于本领域的普通技术人员而言，可以具体情况理解上述术语在本申请中的具体含义。In the description of this application, unless otherwise expressly specified and limited, the terms "connected", "connected" and "fixed" should be understood in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integrated ; It can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood in specific situations.
在本申请中，除非另有明确的规定和限定，第一特征在第二特征之“上”或之“下”可以包括第一和第二特征直接接触，也可以包括第一和第二特征不是直接接触而是通过它们之间的另外的特征接触。而且，第一特征在第二特征“之上”、“上方”和“上面”包括第一特征在第二特征正上方和斜上方，或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”包括第一特征在第二特征正下方和斜下方，或仅仅表示第一特征水平高度小于第二特征。In this application, unless otherwise expressly specified and defined, a first feature "on" or "under" a second feature may include direct contact between the first and second features, or may include the first and second features Not directly but through additional features between them. Also, the first feature being "above", "over" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is level higher than the second feature. The first feature is "below", "below" and "below" the second feature includes the first feature being directly below and diagonally below the second feature, or simply means that the first feature has a lower level than the second feature.
在本实施例的描述中，术语“上”、“下”、“右”、等方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述和简化操作，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本申请的限制。此外，术语“第一”、“第二”仅仅用于在描述上加以区分，并没有特殊的含义。In the description of this embodiment, the terms "upper", "lower", "right", etc. are based on the orientation or positional relationship shown in the accompanying drawings, which are only for convenience of description and simplified operation, rather than indicating Or imply that the device or element referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as a limitation of the present application. In addition, the terms "first" and "second" are only used for distinction in description, and have no special meaning.
实施例一Example 1
图1为本申请实施例提供的核酸检测仪的结构示意图，图2为本申请实施例提供的核酸检测仪的内部结构示意图，图3为本申请实施例提供的核酸检测仪的拆分结构示意图，如图1-图3所示，本实施例提供了一种核酸检测仪，可应用于基因分型、基因突变检测、病原体检测等生物样本分子诊断中，实现样本前处理、核酸提取与纯化、扩增和杂交检测等过程的一体化和自动化操作。FIG. 1 is a schematic structural diagram of a nucleic acid detector provided by an embodiment of the present application, FIG. 2 is a schematic diagram of an internal structure of a nucleic acid detector provided by an embodiment of the present application, and FIG. 3 is a schematic structural diagram of a disassembled nucleic acid detector provided by an embodiment of the present application. 1-3, this embodiment provides a nucleic acid detector, which can be applied to molecular diagnosis of biological samples such as genotyping, gene mutation detection, pathogen detection, etc., to realize sample pretreatment, nucleic acid extraction and purification Integration and automation of processes such as amplification and hybridization detection.
本实施例提供的核酸检测仪包括外壳1以及设置在外壳1内的检测主体。外壳1具有可相对封闭的容纳空间，检测主体设置在容纳空间内，外壳1设置为封闭检测主体，使核酸检测工作在相对密闭、干净的环境中进行，以减少外部环境对试剂的污染。检测主体包括试剂板4、安装架2和设置在安装架2上的振荡加热组件3、磁吸组件6、盖板组件5及移液装置，试剂板4设置在振荡加热组件3上并相对振荡加热组件3固定。其中，试剂板4是进行样本前处理、样本核酸提取和纯化、样本核酸扩增和分子杂交等全部反应的载体；安装架2设置为对检测主体中的结构提供固定和支撑；盖板组件5设置为对设置在试剂板4上的核酸扩增管的自动化封闭和开放；磁吸组件6设置为在核酸提取过程中，进行吸磁和放磁；振荡加热组件3设置为在核酸检测过程中，实现对试剂板4上的液体的加热和降温操作，并实现核酸杂交过程中的液体振荡操作；移液装置设置为实现试剂板4上的液体转移、清洗及废除操作。The nucleic acid detector provided in this embodiment includes a casing 1 and a detection body disposed in the casing 1 . The housing 1 has a relatively closed accommodating space, and the detection body is arranged in the accommodating space. The housing 1 is set to seal the detection body, so that the nucleic acid detection work is performed in a relatively closed and clean environment, so as to reduce the contamination of the reagents by the external environment. The detection main body includes a reagent plate 4, an installation frame 2, an oscillating heating assembly 3, a magnetic suction assembly 6, a cover plate assembly 5 and a pipetting device arranged on the installation frame 2. The reagent plate 4 is arranged on the oscillating heating assembly 3 and oscillates relatively. The heating assembly 3 is fixed. Among them, the reagent plate 4 is a carrier for all reactions such as sample pretreatment, sample nucleic acid extraction and purification, sample nucleic acid amplification and molecular hybridization; the mounting frame 2 is arranged to provide fixation and support for the structure in the detection body; the cover plate assembly 5 It is set to automatically close and open the nucleic acid amplification tube set on the reagent plate 4; the magnetic suction assembly 6 is set to perform magnetic attraction and magnetization during the nucleic acid extraction process; the oscillating heating component 3 is set to be set during the nucleic acid detection process. , realize the heating and cooling operation of the liquid on the reagent plate 4, and realize the liquid shaking operation in the nucleic acid hybridization process;
为方便描述，以图2所示方向建立XYZ坐标系，其中，X方向为试剂板4的布 置方向，Z方向为核酸检测仪的高度方向，Y方向水平设置且根据右手法则确定。For the convenience of description, an XYZ coordinate system is established with the directions shown in Figure 2, wherein the X direction is the arrangement direction of the reagent plate 4, the Z direction is the height direction of the nucleic acid detector, and the Y direction is set horizontally and determined according to the right-hand rule.
图4为本申请实施例提供的外壳1的拆分结构示意图，如图1和4所示，外壳1整体呈六面体结构，包括主壳体11，主壳体11上设置有开口，且外壳1还包括设置于开口处且能够相对主壳体11开合的门体。为方便将样品和试剂等放入容纳空间中，门体包括设置外壳1前侧的第一前门12和第二前门13，第一前门12和第二前门13沿Y方向并排设置，且可单独开合。其中，第一前门12对应试剂板4的位置设置，以方便试剂板4在容纳空间中的取放，第二前门13对应核酸检测仪内的试剂瓶存放区设置，以方便纯水、杂交试剂等瓶装液体在容纳空间中的放置和更换。FIG. 4 is a schematic view of the disassembled structure of the housing 1 provided by the embodiment of the application. As shown in FIGS. 1 and 4 , the housing 1 has a hexahedral structure as a whole, and includes a main housing 11. The main housing 11 is provided with an opening, and the housing 1 It also includes a door body disposed at the opening and capable of opening and closing relative to the main casing 11 . In order to conveniently put samples and reagents into the accommodating space, the door body includes a first front door 12 and a second front door 13 on the front side of the housing 1. The first front door 12 and the second front door 13 are arranged side by side along the Y direction, and can be individually Opening and closing. Among them, the first front door 12 is set corresponding to the position of the reagent plate 4 to facilitate the taking and placing of the reagent plate 4 in the accommodating space, and the second front door 13 is set corresponding to the reagent bottle storage area in the nucleic acid detector to facilitate pure water, hybridization reagents Wait for the placement and replacement of the bottled liquid in the holding space.
在本实施例中，第一前门12的上端与主壳体11枢接，以使第一前门12能够相对主壳体11旋转开合，第二前门13采用按压弹开式的门体结构。在一实施例中，第一前门12还可以是其他侧边与主壳体11连接，第二前门13也可以采用旋转开合的方式与主壳体11连接，本实施例对第一前门12和第二前门13的开门形式和与主壳体11的连接结构并不做具体限制。In this embodiment, the upper end of the first front door 12 is pivotally connected to the main casing 11 so that the first front door 12 can be rotated and opened and closed relative to the main casing 11 , and the second front door 13 adopts a push-and-pop door structure. In one embodiment, the first front door 12 can also be connected to the main casing 11 by other sides, and the second front door 13 can also be connected to the main casing 11 by rotating and closing. The opening form of the second front door 13 and the connection structure with the main casing 11 are not specifically limited.
为方便检测主体在容纳空间中的组装、拆卸和维修，主壳体11与前门对的后侧还开设有供检测主体通过的拆装口，门体还包括可拆卸设置在拆装口处的后门14。在本实施例中，后门14包括沿Y方向并排设置的两扇门体，且每扇门体可单独拆卸，以提高拆装便利性，同时方便对检测主体进行局部结构的维修和更换。在一实施例中，后门14也可以仅包含一扇门体或多扇门体。In order to facilitate the assembly, disassembly and maintenance of the detection body in the accommodating space, the rear side of the pair of the main casing 11 and the front door is also provided with a disassembly port for the detection body to pass through. Backdoor 14. In this embodiment, the rear door 14 includes two door bodies arranged side by side along the Y direction, and each door body can be disassembled separately to improve the convenience of disassembly and assembly, and facilitate the maintenance and replacement of the local structure of the detection body. In one embodiment, the rear door 14 may also include only one door body or multiple door bodies.
在一实施例中，为了对核酸检测仪进行调控，外壳1上设置有设置为实现人机交互的显示屏60，显示屏60为触控显示屏，可用于显示操作界面，以方便操作者根据操作界面控制核酸检测仪的运行，且显示屏60也可以在核酸检测仪的运行过程中，对核酸检测仪的运行状态进行显示。在其他实施例中，第二前门13上贯通开设有安装口131，显示屏60嵌设在安装口131中并与设置在外壳1内部的控制器电连接。In one embodiment, in order to regulate and control the nucleic acid detector, the casing 1 is provided with a display screen 60 configured to realize human-computer interaction. The operation interface controls the operation of the nucleic acid detector, and the display screen 60 can also display the operation state of the nucleic acid detector during the operation of the nucleic acid detector. In other embodiments, an installation opening 131 is formed through the second front door 13 , and the display screen 60 is embedded in the installation opening 131 and is electrically connected to the controller disposed inside the housing 1 .
由于核酸检测过程中存在加热和放热过程，为促使容纳空间中的热量散出，主壳体11于沿Y方向的相对两侧及底部均贯通开设有散热槽，避免热量在容纳空间中堆积。Since there are heating and exothermic processes in the nucleic acid detection process, in order to promote the dissipation of heat in the accommodation space, the main casing 11 is provided with heat dissipation grooves on the opposite sides and the bottom along the Y direction to avoid heat accumulation in the accommodation space .
在一实施例中，主壳体11的内部底面凸设有框架15，框架15的上表面形成设置为承载检测主体的支撑面。框架15上开设有废液收集槽16，设置为收集核酸检测过程中产生的废液，方便废液排出核酸检测仪的外部。废液收集槽16的 槽底设置有排放口，排放口密封插接有排放管，排放管设置为将废液收集槽16中的废液排放至外部的废液瓶中，以方便对废液进行集中处理后向外部环境排放。In one embodiment, a frame 15 is protruded from the inner bottom surface of the main casing 11 , and the upper surface of the frame 15 forms a support surface configured to carry the detection body. The frame 15 is provided with a waste liquid collection tank 16, which is configured to collect waste liquid generated during the nucleic acid detection process, so as to facilitate the waste liquid to be discharged from the outside of the nucleic acid detector. The bottom of the waste liquid collection tank 16 is provided with a discharge port, the discharge port is sealed and inserted with a discharge pipe, and the discharge pipe is arranged to discharge the waste liquid in the waste liquid collection tank 16 to an external waste liquid bottle, so as to facilitate the disposal of the waste liquid. After intensive treatment, it is discharged to the external environment.
如图2和3所示，安装架2包括与XY平面平行的底板21以及设置在底板21上方的支撑架22，支撑架22呈开口朝向底板21的U型结构，支撑架22包括沿Y方向设置的横梁221和设置在横梁221的两端且沿Z方向连接于横梁221和底板21之间的竖梁222。盖板组件5、振荡加热组件3和磁吸组件6均设置在底板21上，移液装置设置在支撑架22上，且支撑架22的U型结构的设置，方便移液装置沿Y方向的运行。As shown in FIGS. 2 and 3 , the mounting frame 2 includes a base plate 21 parallel to the XY plane and a support frame 22 disposed above the base plate 21 . The support frame 22 is in a U-shaped structure with an opening facing the base plate 21 , and the support frame 22 includes a Y direction. The provided cross beam 221 and the vertical beam 222 provided at both ends of the cross beam 221 and connected between the cross beam 221 and the bottom plate 21 along the Z direction. The cover plate assembly 5, the oscillating heating assembly 3 and the magnetic suction assembly 6 are all arranged on the bottom plate 21, the pipetting device is arranged on the support frame 22, and the U-shaped structure of the support frame 22 is arranged to facilitate the pipetting device along the Y direction. run.
为方便安装架2在容纳空间中的取放，底板21下表面向下凸设有支脚23，支脚23沿X方向和Y方向中的任一方向并排设置有至少两个，外壳1的框架15上开设有安装槽，支脚23伸入安装槽中，且支脚23的下端与安装槽的槽底抵接。该种设置，在保证对安装架2的支撑稳定性的同时，能够减小底板21与框架15之间的接触面积。In order to facilitate the picking and placing of the mounting frame 2 in the accommodating space, the lower surface of the bottom plate 21 is protruded downward with a supporting leg 23, and at least two supporting legs 23 are arranged side by side along any direction in the X direction and the Y direction. The frame 15 of the housing 1 An installation groove is formed on the upper part, and the support leg 23 extends into the installation groove, and the lower end of the support leg 23 is in contact with the groove bottom of the installation groove. This arrangement can reduce the contact area between the bottom plate 21 and the frame 15 while ensuring the support stability of the mounting frame 2 .
如图3所示，核酸检测仪，还包括耗材面板50，所述耗材面板50上沿Y方向并排设置有多个试剂板口501，所述试剂板口501沿X方向延伸，图5为本申请实施例提供的试剂板4的结构示意图，如图5所示，试剂板4包括沿X方向延伸的承载主板41，承载主板41上设置有设置为放置核酸扩增管的扩增孔位411、设置为放置杂交固相材料的杂交槽412、设置为放置吸头的吸头腔414以及设置为放置提取试剂的试剂管42及设置为进行提取反应的反应管43，杂交槽412、试剂管42及反应管43分别上端开口，所述承载主板41的下表面搭设于所述耗材面板50上，且所述核酸扩增管、所述杂交槽412、所述反应管43及所述试剂管42伸入所述试剂板口501中。As shown in FIG. 3 , the nucleic acid detector further includes a consumables panel 50 , a plurality of reagent plate ports 501 are arranged side by side along the Y direction on the consumables panel 50 , and the reagent plate ports 501 extend along the X direction, as shown in FIG. 5 . The schematic structural diagram of the reagent plate 4 provided in the application example, as shown in FIG. 5 , the reagent plate 4 includes a carrying main board 41 extending along the X direction, and the carrying main board 41 is provided with an amplification hole 411 configured to place a nucleic acid amplification tube , the hybridization tank 412 set to place the hybridization solid phase material, the pipette cavity 414 set to place the pipette tip, the reagent tube 42 set to place the extraction reagent, and the reaction tube 43 set to carry out the extraction reaction, the hybridization tank 412, the reagent tube 42 and the reaction tube 43 are respectively opened at the upper ends, the lower surface of the carrying main board 41 is placed on the consumables panel 50, and the nucleic acid amplification tube, the hybridization tank 412, the reaction tube 43 and the reagent tube 42 extends into the reagent plate port 501 .
在一实施例中，扩增孔位411设置在试剂板4的一端，方便盖板组件5对核酸扩增管的开口进行密封和开放操作，减小结构干涉。为了方便核酸扩增管在扩增孔位411中的设置，承载主板41上还设置有设置为卡接核酸扩增管管盖的卡口413，卡口413的上端开口并与扩增孔位411的上端开口连通，管盖相对核酸扩增管的管体打开后壳卡入卡口413中，避免管盖翻转。In one embodiment, the amplification hole 411 is arranged at one end of the reagent plate 4 , which facilitates the cover plate assembly 5 to seal and open the opening of the nucleic acid amplification tube and reduce structural interference. In order to facilitate the arrangement of the nucleic acid amplification tube in the amplification hole 411 , the carrier main board 41 is also provided with a bayonet 413 which is configured to be clamped to the cap of the nucleic acid amplification tube. The upper end of the bayonet 413 is open and connected to the amplification hole. The upper end of 411 is open and connected, and the tube cover is opened relative to the tube body of the nucleic acid amplification tube and the shell is snapped into the bayonet 413 to prevent the tube cover from turning over.
在一实施例中，杂交槽412、吸头腔414、试剂管42及反应管43沿X方向向远离扩增孔位411的方向依次布置，图5所示的布置方式为示例性结构，试剂板4上每个孔位、腔室及管的布置位置可以调换，本实施例对此不做具体限制。In one embodiment, the hybridization tank 412, the tip chamber 414, the reagent tube 42 and the reaction tube 43 are arranged in sequence along the X direction in the direction away from the amplification hole 411. The arrangement shown in FIG. 5 is an exemplary structure. The arrangement positions of each hole, chamber and tube on the plate 4 can be exchanged, which is not specifically limited in this embodiment.
在一实施例中，吸头腔414的腔底沿X方向设置有多个吸头管45，多个吸头管45设置为存放多个吸头，保证核酸检测过程中吸头可更换的数量满足使用需求，减小检测试剂间的污染。在本实施例中，吸头腔414内可放置的吸头的个数为三个，但本申请并不限于此。在其他实施例中，试剂管42的个数可以根据待进行的核酸检测项目所需的提取试剂的种类进行具体设定，试剂管42的个数可以但不限定于四个。In one embodiment, the bottom of the pipette cavity 414 is provided with a plurality of pipettes 45 along the X direction, and the pipettes 45 are arranged to store a plurality of pipettes, so as to ensure the number of pipettes that can be replaced during the nucleic acid detection process. Meet the needs of use and reduce the pollution between the detection reagents. In this embodiment, the number of suction tips that can be placed in the tip chamber 414 is three, but the present application is not limited to this. In other embodiments, the number of reagent tubes 42 may be specifically set according to the type of extraction reagent required for the nucleic acid detection item to be performed, and the number of reagent tubes 42 may be but not limited to four.
在一实施例中，承载主板41上设置有样本管44，样本管44设置为存放样品，通过设置样品管44单独存放样品，能够适用于先添加提取试剂再添加样品的核酸检测项目中，提高核酸检测仪的适用性。在其他实施例中，样品也可以直接放置在反应管43中。In one embodiment, the carrying main board 41 is provided with a sample tube 44, and the sample tube 44 is set to store the sample. By setting the sample tube 44 to store the sample separately, it can be applied to the nucleic acid detection project in which the extraction reagent is added first and then the sample is added. Applicability of nucleic acid detectors. In other embodiments, the sample can also be placed directly in the reaction tube 43 .
在一实施例中，承载主板41上设置有废液槽415，废液槽415设置为承接废弃的液体，减小废液移除过程中移液装置所需移动的距离，方便废液的移除操作。在其他实施例中，也可以直接将废弃的液体吸取至试剂板4外部的废液瓶或者废液储槽中。In one embodiment, the carrying main board 41 is provided with a waste liquid tank 415, and the waste liquid tank 415 is set to receive the waste liquid, so as to reduce the distance that the pipetting device needs to move during the waste liquid removal process, and facilitate the transfer of the waste liquid. remove operation. In other embodiments, the waste liquid can also be directly sucked into a waste liquid bottle or a waste liquid storage tank outside the reagent plate 4 .
为在运输过程中对试剂板4内的试剂进行密封和保护，试剂板4的上端面还塑封有密封膜，密封膜可以为塑料膜、铝膜或纸膜等。且塑封膜可相对承载主板41撕离，以使试剂板4放入至外壳1内后，进行正常的核酸检测操作。In order to seal and protect the reagents in the reagent plate 4 during transportation, the upper end surface of the reagent plate 4 is also plastic-sealed with a sealing film, and the sealing film can be a plastic film, an aluminum film or a paper film. And the plastic sealing film can be torn off relative to the carrying main board 41, so that after the reagent plate 4 is put into the housing 1, the normal nucleic acid detection operation can be performed.
图6是本申请实施例提供的振荡加热组件3与试剂板4的结构示意图，图7是本申请实施例提供的振荡加热组件3在一个视角下的结构示意图，图8为图7中I处的局部放大图，图9为本申请实施例提供的拆分结构示意图，如图6-图9所示，振荡加热组件3设置在底板21上且位于横梁221的下方，多个试剂板4沿Y方向并排设置在振荡加热组件3的上方，振荡加热组件3设置为对试剂板4中的液体进行加热，或带动试剂板4沿X方向往复运动。在一实施例中，试剂板4也可以仅设置一个。FIG. 6 is a schematic structural diagram of the oscillating heating assembly 3 and the reagent plate 4 provided by the embodiment of the present application, FIG. 7 is a schematic structural diagram of the oscillating heating assembly 3 provided by the embodiment of the present application from a viewing angle, and FIG. 8 is the position I in FIG. 7 . 9 is a schematic view of the split structure provided by the embodiment of the present application, as shown in FIGS. 6-9 , the oscillating heating assembly 3 is arranged on the bottom plate 21 and is located below the beam 221, and a plurality of reagent plates 4 along the The oscillating heating assemblies 3 are arranged side by side in the Y direction, and the oscillating heating assemblies 3 are arranged to heat the liquid in the reagent plate 4 or drive the reagent plate 4 to reciprocate along the X direction. In one embodiment, only one reagent plate 4 may be provided.
在一实施例中，振荡加热组件3包括由上至下依次设置的热处理单元31、隔热单元33和振荡单元32，热处理单元31的上方固设有试剂板4。通过采用隔热单元33隔离热处理单元31和振荡单元32，能够提高热处理单元31的处理效率，同时，避免热量向下传递至振荡单元32中，保证振荡单元32的使用可靠性。In one embodiment, the oscillating heating assembly 3 includes a heat treatment unit 31 , a heat insulating unit 33 and an oscillation unit 32 arranged in sequence from top to bottom, and a reagent plate 4 is fixed above the heat treatment unit 31 . Using the heat insulation unit 33 to isolate the heat treatment unit 31 and the oscillation unit 32 can improve the treatment efficiency of the heat treatment unit 31 , and at the same time, prevent the heat from being transferred downward to the oscillation unit 32 and ensure the use reliability of the oscillation unit 32 .
在一实施例中，热处理单元31包括沿X方向并排且间隔设置的多个加热单元，每个加热单元沿Y方向延伸。在本实施例中，多个加热单元包括设置为对核酸扩增管进行加热的核酸扩增管加热块311、设置为对杂交槽412进行加热的杂交槽 加热板314、设置为对反应管43进行加热的反应管加热块312以及设置为对提取试剂进行加热的提取试剂加热块313，且核酸扩增管加热块311、杂交槽加热板314、反应管加热块312及提取试剂加热块313沿X方向的排布位置与试剂板4上扩增孔位411、杂交槽412、反应管43及试剂管42的位置一一对应。In one embodiment, the heat treatment unit 31 includes a plurality of heating units arranged side by side and at intervals along the X direction, and each heating unit extends along the Y direction. In this embodiment, the plurality of heating units include a nucleic acid amplification tube heating block 311 configured to heat the nucleic acid amplification tube, a hybridization tank heating plate 314 configured to heat the hybridization tank 412 , and a reaction tube 43 configured to heat The reaction tube heating block 312 for heating and the extraction reagent heating block 313 configured to heat the extraction reagent, and the nucleic acid amplification tube heating block 311, the hybridization tank heating plate 314, the reaction tube heating block 312 and the extraction reagent heating block 313 are The arrangement positions in the X direction correspond to the positions of the amplification wells 411 , the hybridization tank 412 , the reaction tubes 43 and the reagent tubes 42 on the reagent plate 4 one by one.
在本实施例中，试剂管42设置有多个，提取试剂加热块313对应位于中间的一个试剂管42设置，以使热量可以扩散至周围其余试剂管42中。在其他实施例中，提取试剂加热块313的个数也可以与试剂管42的个数相对应。In this embodiment, a plurality of reagent tubes 42 are provided, and the extraction reagent heating block 313 is provided corresponding to one of the reagent tubes 42 in the middle, so that heat can be diffused to the remaining reagent tubes 42 around. In other embodiments, the number of extraction reagent heating blocks 313 may also correspond to the number of reagent tubes 42 .
为方便对试剂板4上的多个管或槽进行相对固定，每一加热块的上端面开设有设置为插入对应管结构的管插孔319，当试剂板4相对振荡加热组件3固定时，核酸扩增管、试剂管42及反应管43插入对应加热块的管插孔319中，且杂交槽412的槽底抵接于杂交槽加热板314上。该种设置方式，能够提高每个加热单元对对应管或槽结构的加热效率，减少热量流失，且进一步地保证试剂板4与振荡加热组件3之间的位置相对稳定性。In order to facilitate the relative fixing of the plurality of tubes or grooves on the reagent plate 4, the upper end face of each heating block is provided with a tube jack 319 configured to be inserted into the corresponding tube structure. When the reagent plate 4 is fixed relative to the oscillating heating assembly 3, The nucleic acid amplification tube, the reagent tube 42 and the reaction tube 43 are inserted into the tube insertion holes 319 of the corresponding heating block, and the bottom of the hybridization tank 412 abuts on the heating plate 314 of the hybridization tank. This arrangement can improve the heating efficiency of each heating unit to the corresponding tube or groove structure, reduce heat loss, and further ensure the relative stability of the position between the reagent plate 4 and the oscillating heating assembly 3 .
在一实施例中，每个加热块沿长度方向的一侧面设置有加热膜315，加热膜315设置为连接电源，为对应加热块提供热源，即，对加热块进行加热。采用加热块与加热膜315分离的设置，能够简化加热块的结构设置，避免对加热块直接通电加热导致的加热块结构复杂，不易加工加工等问题。在一实施例中，加热膜315设置在加热块的与YZ平行的侧面上，避免加热膜315与位于下方的隔热单元33直接接触。能够进行电加热的加热膜315和加热板的结构为本领域的常规结构，此处不再赘述。In one embodiment, a heating film 315 is disposed on one side of each heating block along the length direction, and the heating film 315 is configured to be connected to a power source to provide a heat source for the corresponding heating block, that is, to heat the heating block. By adopting the arrangement that the heating block and the heating film 315 are separated, the structure and arrangement of the heating block can be simplified, and the problems such as complicated structure of the heating block caused by direct electric heating of the heating block and difficult processing can be avoided. In one embodiment, the heating film 315 is disposed on the side of the heating block parallel to YZ, so as to prevent the heating film 315 from directly contacting the heat insulating unit 33 located below. The structures of the heating film 315 and the heating plate that can be electrically heated are conventional structures in the field, and will not be repeated here.
由于振荡加热组件3上方沿Y方向并排设置有多个试剂板4，每个加热块上均沿Y方向设置有多个管插孔319，且每个加热块上的管插孔319的个数与试剂板4的个数和位置一一对应，以实现对多次核酸检测实验的同步进行。在一实施例中，核酸扩增管加热块311和提取试剂加热块313上的管插孔319为圆柱形通孔或盲孔，且上端开口端可设置方便管插入的倒角。Since a plurality of reagent plates 4 are arranged side by side along the Y direction above the oscillating heating assembly 3, each heating block is provided with a plurality of tube insertion holes 319 along the Y direction, and the number of tube insertion holes 319 on each heating block is One-to-one correspondence with the number and position of the reagent plates 4, so as to realize the synchronization of multiple nucleic acid detection experiments. In one embodiment, the tube insertion holes 319 on the nucleic acid amplification tube heating block 311 and the extraction reagent heating block 313 are cylindrical through holes or blind holes, and the upper open end can be provided with a chamfer to facilitate tube insertion.
反应管加热块312上的管插孔319的横截面积为开口背离核酸扩增管加热块311的圆弧形结构，且该管插孔319贯通反应管加热块312的上下端及一侧侧壁，以方便热量的散发，且有利于减轻反应管加热块312的重量，同时，反应管加热块312上的管插孔319设置为上述结构，也有利于磁吸组件6对反应管43内的反应液进行吸磁和放磁反应，减小磁吸组件6与反应管43之间的结构障碍。The cross-sectional area of the tube insertion hole 319 on the reaction tube heating block 312 is an arc-shaped structure with an opening away from the nucleic acid amplification tube heating block 311 , and the tube insertion hole 319 penetrates the upper and lower ends and one side of the reaction tube heating block 312 wall, in order to facilitate the dissipation of heat, and help to reduce the weight of the reaction tube heating block 312, and at the same time, the pipe jack 319 on the reaction tube heating block 312 is set to the above structure, which is also beneficial for the magnetic attraction assembly 6 to the reaction tube 43. The reacting liquid of the ferromagnetic material undergoes magnetic attraction and expulsion reactions, thereby reducing the structural obstacle between the magnetic attraction component 6 and the reaction tube 43 .
在一实施例中，为减轻振荡加热组件3的整体重量，相邻两个管插孔319之 间设置有减重槽3110，以减轻加热块的整体重量，从而减轻振荡加热组件3的整体重量。在其他实施例中，减重槽3110沿Z方向贯通加热块。In one embodiment, in order to reduce the overall weight of the oscillating heating assembly 3 , a weight-reducing groove 3110 is provided between two adjacent pipe insertion holes 319 to reduce the overall weight of the heating block, thereby reducing the overall weight of the oscillating heating assembly 3 . . In other embodiments, the weight reduction groove 3110 penetrates the heating block in the Z direction.
在一实施例中，为了满足核酸扩增反应过程中的制冷需求，核酸扩增管加热块311沿自身长度方向设置的一侧设置有制冷单元317，制冷单元317设置为对核酸扩增管加热块311进行制冷处理，以对核酸扩增管中的试剂进行制冷。在本实施例中，制冷单元317包括与加热膜315相对设置的制冷片。制冷片的结构和制冷原理为本领域的常规设置，本实施例不再进行赘述。且在其他实施例中，制冷片沿核酸扩增管加热块311的长度方向并排设置有多个，以满足对整条核酸扩增管加热块311的制冷作用。In one embodiment, in order to meet the refrigeration requirement during the nucleic acid amplification reaction, a refrigeration unit 317 is provided on one side of the nucleic acid amplification tube heating block 311 along its length direction, and the refrigeration unit 317 is set to heat the nucleic acid amplification tube. Block 311 performs a cooling process to cool the reagents in the nucleic acid amplification tube. In this embodiment, the cooling unit 317 includes a cooling sheet disposed opposite to the heating film 315 . The structure and refrigeration principle of the refrigerating sheet are conventional settings in the field, and are not repeated in this embodiment. And in other embodiments, a plurality of cooling sheets are arranged side by side along the length direction of the nucleic acid amplification tube heating block 311 to satisfy the cooling effect on the entire nucleic acid amplification tube heating block 311 .
由于核酸扩增反应中存在放热过程，为使热量尽快散出，核酸扩增管加热块311的一侧还设置有散热单元316。在本实施例中，为提高散热效果，散热单元316包括上下层叠设置的散热器3161和散热风扇3162，且散热风扇3162连接与散热器3161的下方。Since there is an exothermic process in the nucleic acid amplification reaction, in order to dissipate the heat as soon as possible, a heat dissipation unit 316 is further provided on one side of the heating block 311 of the nucleic acid amplification tube. In this embodiment, in order to improve the heat dissipation effect, the heat dissipation unit 316 includes a radiator 3161 and a heat dissipation fan 3162 that are stacked up and down, and the heat dissipation fan 3162 is connected to the lower part of the heat sink 3161 .
散热器3161包括与YZ平面平行的竖板部31611、水平设置且与竖板部31611连接的横板部31612以及与竖板部31611平行且与横板部31612连接的翅片部31613。竖板部31611与核酸扩增管加热块311可拆卸连接并位于与加热膜315相对的一侧，且制冷片夹设在竖板部31611与核酸扩增管加热块311之间；竖板部31611沿竖直方向的两侧均设置有横板部31612，横板部3162沿远离核酸扩增管加热块311的方向延伸，且位于下方的横板部31612与散热风扇3162可拆卸连接；翅片部31613连接于两个横板部31612之间，且沿X方向并排且间隔设置有多个，以增强散热效果。The radiator 3161 includes a vertical plate portion 31611 parallel to the YZ plane, a horizontal plate portion 31612 arranged horizontally and connected to the vertical plate portion 31611 , and a fin portion 31613 parallel to the vertical plate portion 31611 and connected to the horizontal plate portion 31612 . The vertical plate portion 31611 is detachably connected to the nucleic acid amplification tube heating block 311 and is located on the opposite side of the heating film 315, and the cooling plate is sandwiched between the vertical plate portion 31611 and the nucleic acid amplification tube heating block 311; the vertical plate portion Both sides of the 31611 along the vertical direction are provided with horizontal plate portions 31612, the horizontal plate portions 3162 extend in a direction away from the nucleic acid amplification tube heating block 311, and the horizontal plate portion 31612 located below is detachably connected to the cooling fan 3162; The sheet portions 31613 are connected between the two horizontal plate portions 31612, and are arranged side by side along the X-direction at intervals to enhance the heat dissipation effect.
在一实施例中，横板部31612沿核酸扩增管加热块311的长度方向间隔设置有多个，以增加散热效果，且位于下方的每个横板部31612上可拆卸连接有一个散热风扇3162。该种设置，更有利于提高散热效果。在其他实施例中，横板部31612也可以沿核酸扩增管加热块311的长度方向设置一整条。In one embodiment, a plurality of horizontal plate portions 31612 are arranged at intervals along the length direction of the nucleic acid amplification tube heating block 311 to increase the heat dissipation effect, and a cooling fan is detachably connected to each horizontal plate portion 31612 located below. 3162. This arrangement is more conducive to improving the heat dissipation effect. In other embodiments, the horizontal plate portion 31612 can also be provided with a whole piece along the length direction of the nucleic acid amplification tube heating block 311 .
隔热单元33包括多个分别设置于加热单元下方的隔热子单元，隔热子单元与对应的加热单元可拆卸连接，设置为对每个加热单元进行隔热处理，提高隔热效果，且能够避免每个加热块处的热量流失，提高各加热单元在加热时的加热效果和加热效率。The heat insulating unit 33 includes a plurality of heat insulating sub-units respectively arranged below the heating unit, the heat insulating sub-units are detachably connected to the corresponding heating units, and are arranged to perform heat insulating treatment on each heating unit to improve the heat insulating effect, and The heat loss at each heating block can be avoided, and the heating effect and heating efficiency of each heating unit during heating can be improved.
在本实施例中，位于核酸扩增管加热块311和提取试剂加热块313下方的隔热子单元为隔热块331，位于反应管加热块312下方的为隔热板335，隔热板335 和隔热块331均由隔热材料制成，且每个隔热子单元均沿Y方向延伸以覆盖对应加热块的长度范围。且为提高隔热块331或隔热板对对应加热块的连接定位，隔热块331或隔热板上可设置有对加热块进行定位的定位槽。In this embodiment, the heat insulating subunit located under the nucleic acid amplification tube heating block 311 and the extraction reagent heating block 313 is the heat insulating block 331 , and the heat insulating subunit located under the reaction tube heating block 312 is the heat insulating plate 335 , and the heat insulating plate 335 Both the heat insulating block 331 and the heat insulating block 331 are made of heat insulating material, and each heat insulating subunit extends along the Y direction to cover the length range of the corresponding heating block. And in order to improve the connection and positioning of the heat insulating block 331 or the heat insulating plate to the corresponding heating block, the heat insulating block 331 or the heat insulating plate may be provided with a positioning groove for positioning the heating block.
杂交槽加热板314下方的隔热子单元包括多个隔热柱332，隔热柱332竖直设置，且沿Y方向间隔设置有多组，每组至少包括沿X方向设置间隔设置的两个隔热柱332。在一实施例中，隔热柱332由隔热材料制成。The thermal insulation subunit below the hybridization tank heating plate 314 includes a plurality of thermal insulation columns 332, the thermal insulation columns 332 are arranged vertically, and are arranged in multiple groups at intervals along the Y direction, and each group includes at least two arranged at intervals along the X direction. Insulation post 332. In one embodiment, the insulating posts 332 are made of insulating material.
隔热单元33还包括位于所有隔热子单元下方的隔热载板333，每个隔热子单元设置在隔热载板333上，并与隔热载板333可拆卸连接。为进一步地避免热量传递至振荡单元32，隔热单元33还包括位于隔热载板333下方的隔热支柱334，隔热支柱334沿Y方向间隔设置有多组，且每组至少包括沿X方向间隔设置的多个隔热支柱334。隔热支柱334的设置，能够增加热处理单元31和振荡单元32之间的间隙，且能够促进空气在隔热载板333和振荡单元32之间的流动，方便热量的扩散。在一实施例中，隔热支柱334和隔热载板333采用隔热材料制成，但也可以采用导热系数低的材料制成。The heat insulating unit 33 further includes a heat insulating support plate 333 located below all the heat insulating subunits, and each heat insulating subunit is arranged on the heat insulating support plate 333 and is detachably connected with the heat insulating support plate 333 . In order to further prevent heat from being transferred to the oscillating unit 32, the heat insulating unit 33 further includes heat insulating struts 334 located under the heat insulating support plate 333. The heat insulating struts 334 are spaced in a plurality of groups along the Y direction, and each group at least includes an area along the X direction. The plurality of thermal insulation pillars 334 are arranged at intervals in the direction. The disposition of the heat insulating support 334 can increase the gap between the heat treatment unit 31 and the oscillating unit 32 , and can promote the flow of air between the heat insulating carrier plate 333 and the oscillating unit 32 to facilitate the diffusion of heat. In one embodiment, the thermal insulation pillars 334 and the thermal insulation carrier plate 333 are made of thermal insulation materials, but may also be made of materials with low thermal conductivity.
振荡单元32包括与XY平面平行的支撑板321、位于支撑板321下方的X向导向组件322及设置为驱动支撑板321沿X方向往复运动的振荡驱动单元(未示出)。隔热支柱334的下端与支撑板321可拆卸连接。为了减轻振荡加热组件3的整体重量，支撑板321上设置有多个减重孔。The oscillation unit 32 includes a support plate 321 parallel to the XY plane, an X-direction guide assembly 322 located below the support plate 321 and an oscillation drive unit (not shown) configured to drive the support plate 321 to reciprocate in the X direction. The lower ends of the heat insulating pillars 334 are detachably connected to the support plate 321 . In order to reduce the overall weight of the oscillating heating assembly 3 , the support plate 321 is provided with a plurality of weight-reducing holes.
X向导向组件322设置在支撑板321和底板21之间，设置为实现支撑板321相对底板21沿X方向运行的导向。在本实施例中，X向导向组件322包括沿X方向设置在底板21上的X向导轨3221及设置在支撑板321下表面且与的X向导轨3221滑动配合的X向滑块3222，X向导轨3221和X向滑块3222的结构可参考相关技术中的直线导轨的结构，此处不再赘述。且为提高支撑板321的运动平稳性，X向导向组件322沿Y方向至少间隔设置有两组。The X-direction guide assembly 322 is disposed between the support plate 321 and the bottom plate 21 , and is configured to guide the support plate 321 relative to the bottom plate 21 along the X direction. In this embodiment, the X-direction guide assembly 322 includes an X-direction guide rail 3221 arranged on the bottom plate 21 along the X-direction and an X-direction sliding block 3222 arranged on the lower surface of the support plate 321 and slidably matched with the X-direction guide rail 3221. For the structures of the directional guide rail 3221 and the X-directional sliding block 3222, reference may be made to the structure of the linear guide rail in the related art, which will not be repeated here. In addition, in order to improve the motion stability of the support plate 321, at least two groups of the X-direction guide components 322 are arranged at intervals along the Y-direction.
为避免支撑板321沿X方向往复运动时脱离X向导轨322，X向导轨322的两端设置有限位块323，X向滑块3222在滑动至X向导轨322末端时与限位块323抵接实现限位。在一实施例中，限位块323由橡胶等弹性材料制成，以在实现限位的同时，避免滑块与限位块323硬碰撞造成的振动、变形或噪音大等问题。In order to prevent the support plate 321 from separating from the X-direction guide rail 322 when reciprocating along the X-direction, limit blocks 323 are provided at both ends of the X-direction guide rail 322, and the X-direction slider 3222 contacts the limit block 323 when sliding to the end of the X-direction guide rail 322. Connect to achieve limit. In one embodiment, the limiting block 323 is made of elastic material such as rubber, so as to avoid vibration, deformation or loud noise caused by the hard collision between the slider and the limiting block 323 while realizing the limiting.
振荡驱动单元包括X向丝杆电机(未示出)，X向丝杆电机的固定端与底板21固定连接，X向丝杆电机的驱动端与支撑板321连接。由于支撑板321沿Y方向的尺寸较长，为提高驱动稳定性和可靠性，支撑板321的一侧设置有固定支架324， 固定支架324沿Y方向间隔设置有至少两个，两个固定支架324连接于同一沿Y方向设置的转接件，转接件与X向丝杆电机连接。转接件和固定支架324的设置，能够抬高设置为与X向丝杆电机的连接位置，提高驱动可靠性的同时，方便X向丝杆电机的设置。The oscillating driving unit includes an X-direction screw motor (not shown), the fixed end of the X-direction screw motor is fixedly connected to the base plate 21 , and the driving end of the X-direction screw motor is connected to the support plate 321 . Since the dimension of the support plate 321 along the Y direction is relatively long, in order to improve the driving stability and reliability, one side of the support plate 321 is provided with a fixing bracket 324, and at least two fixing brackets 324 are arranged at intervals along the Y direction. 324 is connected to the same adapter arranged along the Y direction, and the adapter is connected to the X-direction screw motor. The arrangement of the adapter and the fixing bracket 324 can be elevated and set to the connection position with the X-direction screw motor, which improves the driving reliability and facilitates the setting of the X-direction screw motor.
在一实施例中，支撑板321沿X方向往复运动的行程为10-20mm。为对振荡加热组件3的振荡行程进行监控，振荡加热组件3还包括设置为检测振荡加热组件3沿X方向的运动位移的位移检测组件20。在本实施例中，位移检测组件20包括设置在支撑板321一侧的光电开关201以及设置在底板21上的光电感应片202。通过光电开关201检测位移的结构和原理，为本领域的常规设置，此处不再赘述。在其他实施例中，还可以设置其他能够用于检测振荡位移的检测装置，如光栅尺、距离传感器等。In one embodiment, the reciprocating stroke of the support plate 321 along the X direction is 10-20 mm. In order to monitor the oscillating stroke of the oscillating heating assembly 3 , the oscillating heating assembly 3 further includes a displacement detecting assembly 20 configured to detect the movement and displacement of the oscillating heating assembly 3 along the X direction. In this embodiment, the displacement detection assembly 20 includes a photoelectric switch 201 disposed on one side of the support plate 321 and a photoelectric induction sheet 202 disposed on the bottom plate 21 . The structure and principle of detecting displacement by the photoelectric switch 201 are conventional settings in the field, and will not be repeated here. In other embodiments, other detection devices that can be used to detect the oscillating displacement, such as a grating ruler, a distance sensor, etc., may also be provided.
由于振荡加热组件3在快速振荡过程中会与底板21产生发热，为避免热量的堆积，支撑板321和底板21中的至少一个对应X向导向组件322的位置设置有风扇，用于散热。Since the oscillating heating assembly 3 generates heat with the bottom plate 21 during rapid oscillation, in order to avoid heat accumulation, at least one of the support plate 321 and the bottom plate 21 is provided with a fan at a position corresponding to the X-direction guide assembly 322 for heat dissipation.
图10为本申请实施例提供的盖板组件5在一个视角下的结构示意图，图11为本申请实施例提供的盖板组件5的另一个视角下的结构示意图，图12为本申请实施例提供的盖板组件5的拆分结构示意图，如图10-图12所示，盖板组件5设置在振荡加热组件3的一侧，且位于试剂板4设置有扩增孔位411的一端外部，设置为对设置在扩增孔位411中的核酸扩增管的开口进行封闭和开启。在一实施例中，盖板组件5包括设置为封闭核酸扩增管的开口的盖封组件51和设置为驱动盖封组件51沿朝向或远离扩增孔位411运动的盖板驱动组件52。在本实施例中，盖板驱动组件52驱动盖板组件5沿竖直方向运动，以提高对核酸扩增管开口的封闭或开放便利性。在其他实施例中，盖板驱动组件52也可以是驱动盖封组件51转动或相对水平方向倾斜移动，以使盖封组件52在密封核酸扩增管管口的第一位置和开启核酸扩增管管口的第二位置间切换。FIG. 10 is a schematic structural diagram of the cover plate assembly 5 provided by the embodiment of the application from one perspective, FIG. 11 is a schematic structural diagram of the cover plate assembly 5 provided by the embodiment of the application from another perspective, and FIG. 12 is an embodiment of the application Provided is a schematic diagram of the disassembled structure of the cover plate assembly 5. As shown in FIGS. 10-12 , the cover plate assembly 5 is arranged on one side of the oscillating heating assembly 3, and is located outside the end of the reagent plate 4 where the amplification hole position 411 is arranged. , set to close and open the opening of the nucleic acid amplification tube set in the amplification hole position 411 . In one embodiment, the cover plate assembly 5 includes a cover plate assembly 51 configured to close the opening of the nucleic acid amplification tube and a cover plate drive assembly 52 configured to drive the cover plate assembly 51 to move toward or away from the amplification hole 411 . In this embodiment, the cover plate driving assembly 52 drives the cover plate assembly 5 to move in the vertical direction, so as to improve the convenience of closing or opening the opening of the nucleic acid amplification tube. In other embodiments, the cover plate driving component 52 may also drive the cover sealing component 51 to rotate or move tilted relative to the horizontal direction, so that the cover sealing component 52 is in the first position of sealing the mouth of the nucleic acid amplification tube and starts nucleic acid amplification Toggle between the second position of the nozzle of the pipe.
盖封组件51包括盖板支架及盖封件514，盖板支架包括垂直连接的顶盖板511和竖连接板512，顶盖板511水平设置且位于扩增孔位411的正上方，竖连接板512的上侧与顶盖板511连接，竖连接板512的下侧与盖板驱动组件52连接，且竖连接板512位于试剂板4的一端外侧。The cover assembly 51 includes a cover bracket and a cover seal 514. The cover bracket includes a vertically connected top cover 511 and a vertical connection plate 512. The top cover 511 is horizontally arranged and located directly above the amplification hole 411, and is vertically connected. The upper side of the plate 512 is connected to the top cover plate 511 , the lower side of the vertical connecting plate 512 is connected to the cover plate driving assembly 52 , and the vertical connecting plate 512 is located outside one end of the reagent plate 4 .
盖封件514设置在顶盖板511内表面且远离竖连接板512的一侧，且盖封件514正对扩增孔位411设置，以使盖封件514向下运动对位于扩增孔位411的核酸 扩增管的开口进行封闭，向上运动时能够对核酸扩增管的开口进行开放。在一实施例中，盖封件514由硅胶、PE材料、PP材料等不能吸附核酸且具有一定弹性的材料制成，以实现对开口的紧密密封。The cover seal 514 is disposed on the inner surface of the top cover plate 511 and away from the side of the vertical connection plate 512, and the cover seal 514 is disposed facing the amplification hole 411, so that the cover seal 514 moves downward to be positioned in the amplification hole The opening of the nucleic acid amplification tube at position 411 is closed, and the opening of the nucleic acid amplification tube can be opened when moving upward. In one embodiment, the cover sealing member 514 is made of materials such as silica gel, PE material, PP material, which cannot adsorb nucleic acid and has a certain elasticity, so as to achieve tight sealing of the opening.
在一实施例中，盖封件514呈沿片状结构，能够减小盖封件514的尺寸。进一步地，盖封件514沿Y方向延伸且可覆盖多个试剂板4的扩增孔位411，以方便盖封件514的设置。单个盖封件514可覆盖的扩增孔位411的个数可以根据核酸检测仪能够一次性同步进行的核酸检测试验的个数进行确定，以方便盖封件514的拆装和更换。In one embodiment, the cover seal 514 has a sheet-like structure, which can reduce the size of the cover seal 514 . Further, the cover seal 514 extends along the Y direction and can cover the amplification wells 411 of the plurality of reagent plates 4 , so as to facilitate the setting of the cover seal 514 . The number of amplification holes 411 that can be covered by a single cover seal 514 can be determined according to the number of nucleic acid detection tests that the nucleic acid detector can perform synchronously at one time, so as to facilitate the disassembly and replacement of the cover seal 514 .
为方便盖板驱动组件52和盖封组件51的连接，盖封组件51还包括水平设置的横连接板513，横连接板513的第一侧与竖连接板512的下侧连接，横连接板513的第二侧沿远离试剂板4的方向延伸。在本实施例中，盖板驱动组件52包括盖板电机321、与盖板电机521的输出端连接且竖直设置的盖板丝杆522、套设在盖板丝杆522上的盖板螺母座523，盖板电机521的固定部与安装架2连接，盖板螺母座523与横连接板513连接。In order to facilitate the connection between the cover plate drive assembly 52 and the cover seal assembly 51, the cover seal assembly 51 further includes a horizontally arranged horizontal connecting plate 513. The first side of the horizontal connecting plate 513 is connected to the lower side of the vertical connecting plate 512. The second side of 513 extends in a direction away from the reagent plate 4 . In this embodiment, the cover drive assembly 52 includes a cover motor 321 , a cover screw 522 connected to the output end of the cover motor 521 and arranged vertically, and a cover nut sleeved on the cover screw 522 The seat 523, the fixing part of the cover motor 521 is connected with the mounting frame 2, and the cover nut seat 523 is connected with the horizontal connecting plate 513.
为方便盖板电机521与安装架2的连接，盖板组件5还包括盖板电机座53。盖板电机座53包括与与YZ平面平行的安装板531，安装板531远离顶盖板511的一侧沿Y方向间隔设置有两个安装座532，每个安装座532与底板21可拆卸连接，以提高与底板21的连接稳定性。安装板531上连接有水平设置的电机支板533，电机支板533与安装板531垂直并与定盖板电机521可拆卸连接。安装板531上还开设有供盖板丝杆522穿过的避让孔。In order to facilitate the connection between the cover motor 521 and the mounting frame 2 , the cover assembly 5 further includes a cover motor base 53 . The cover plate motor seat 53 includes a mounting plate 531 parallel to the YZ plane. The side of the mounting plate 531 away from the top cover plate 511 is provided with two mounting seats 532 at intervals along the Y direction, and each mounting seat 532 is detachably connected to the bottom plate 21 , in order to improve the connection stability with the bottom plate 21 . A horizontally arranged motor support plate 533 is connected to the mounting plate 531 . The motor support plate 533 is perpendicular to the mounting plate 531 and is detachably connected to the fixed cover motor 521 . The mounting plate 531 is also provided with an escape hole for the cover plate screw 522 to pass through.
在一实施例中，为进一步地提高盖板组件5的结构稳定性，安装板531贯通开设有穿板口5311。横连接板513包括沿X方向连接的第一横板部5131和第二横板部5132，第一横板部5131的第一侧与竖连接板512连接，第一横板部5131的第二侧与第二横板部5132连接，第二横板部5132沿Y方向的长度大于第一横板部5131沿Y方向的长度，且第一横板部5131穿设于穿板口5311并能在穿板口5311中竖直升降。In one embodiment, in order to further improve the structural stability of the cover plate assembly 5 , the mounting plate 531 is provided with a plate penetration opening 5311 therethrough. The horizontal connecting plate 513 includes a first horizontal plate portion 5131 and a second horizontal plate portion 5132 connected along the X direction, the first side of the first horizontal plate portion 5131 is connected with the vertical connecting plate 512, the second horizontal plate portion 5131 The side is connected with the second transverse plate portion 5132, the length of the second transverse plate portion 5132 in the Y direction is greater than the length of the first transverse plate portion 5131 in the Y direction, and the first transverse plate portion 5131 is penetrated through the plate hole 5311 and can Vertically lift and lower in the hole 5311.
为提高盖封组件51沿Z方向的运行平稳性，盖封组件51包括为盖封组件51的Z向运动进行导向的盖板导向组件54。盖板导向组件54包括沿竖直方向间隔设置在安装板531上的两个光轴座541以及竖直设置的光轴542，光轴542的两端分别插设于两个光轴座541上。第二横板部5132上开设有光轴孔5133，光轴542穿设于光轴孔5133中，以使横连接板513沿光轴542竖直升降。在一实施例中，横连 接板513对应光轴孔5133的位置连接有直线轴承543，光轴542穿设于直线轴承543内，降低导向时的摩擦，提高导向可靠性。为进一步地提高导向的稳定性和可靠性，盖板导向组件54沿Y方向间隔设置有两个，且两个盖板导向组件54分别设置于盖板驱动组件52的两侧。In order to improve the running stability of the cover sealing assembly 51 along the Z direction, the cover sealing assembly 51 includes a cover plate guide assembly 54 for guiding the Z direction movement of the cover sealing assembly 51 . The cover guide assembly 54 includes two optical axis seats 541 arranged on the mounting plate 531 at intervals in the vertical direction and an optical axis 542 arranged vertically. Both ends of the optical axis 542 are respectively inserted into the two optical axis seats 541 . The second horizontal plate portion 5132 is provided with an optical axis hole 5133 , and the optical axis 542 passes through the optical axis hole 5133 , so that the horizontal connecting plate 513 can be vertically lifted and lowered along the optical axis 542 . In one embodiment, a linear bearing 543 is connected to the position of the horizontal connecting plate 513 corresponding to the optical axis hole 5133, and the optical axis 542 is inserted into the linear bearing 543, which reduces the friction during guiding and improves the guiding reliability. In order to further improve the stability and reliability of the guide, two cover guide assemblies 54 are arranged at intervals along the Y direction, and the two cover guide assemblies 54 are respectively disposed on both sides of the cover drive assembly 52 .
为检测和控制盖封组件51的运行行程，盖板组件5还包括位移检测组件20。在本实施例中，盖板位移检测组件20包括设置在横连接板513上的光电感应片202和设置在安装板531的光电开关201，光电开关201和光电感应片202正对设置。在其他实施例中，还可以采用其他检测装置检测盖封组件51的运行行程，此处不再一一赘述。In order to detect and control the running stroke of the cover assembly 51 , the cover plate assembly 5 further includes a displacement detection assembly 20 . In this embodiment, the cover plate displacement detection assembly 20 includes a photoelectric sensor sheet 202 arranged on the horizontal connecting plate 513 and a photoelectric switch 201 arranged on the mounting plate 531 . In other embodiments, other detection devices may also be used to detect the running stroke of the capping assembly 51 , which will not be repeated here.
为减轻盖板组件的重量，顶盖板511、竖连接板512、横连接板513及安装板531上分别开设有减重孔。In order to reduce the weight of the cover plate assembly, the top cover plate 511 , the vertical connecting plate 512 , the horizontal connecting plate 513 and the mounting plate 531 are respectively provided with weight reducing holes.
图13为本申请实施例提供的磁吸组件在一个视角下的结构示意图，图14为本申请实施例提供的磁吸组件在另一个视角下的结构示意图，图15为本申请实施例提供的磁吸组件的拆分结构示意图，图16为图15中J处的局部放大图，如13-图16所述，磁吸组件6包括磁棒组件61和驱动磁棒组件61沿朝向或远离试剂板4方向运动的磁棒驱动组件62。在本实施例中，磁棒组件61位于试剂板4的下方，磁棒驱动组件62设置为驱动磁棒组件61竖直升降。该种设置，能够提高结构紧凑性，且方便磁吸组件6对反应管43中的混合液中的磁珠进行磁吸和放磁反应。在其他实施例中，磁棒组件61也可以采用其他运动方向向靠近或远离反应管43运动，如水平运行或倾斜运动等。FIG. 13 is a schematic structural diagram of the magnetic attraction assembly provided by the embodiment of the application from one perspective, FIG. 14 is a schematic structural diagram of the magnetic attraction assembly provided by the embodiment of the application from another perspective, and FIG. 15 is provided by the embodiment of the application. A schematic diagram of the disassembled structure of the magnetic attraction assembly, Fig. 16 is a partial enlarged view of J in Fig. 15, as described in Fig. 13-Fig. 16, the magnetic attraction assembly 6 includes a magnetic rod assembly 61 and a driving magnetic rod assembly 61 along the direction toward or away from the reagent The magnet bar drive assembly 62 for movement of the plate 4 in the direction. In this embodiment, the magnet bar assembly 61 is located below the reagent plate 4 , and the magnet bar driving assembly 62 is configured to drive the magnet bar assembly 61 to rise and fall vertically. This arrangement can improve the compactness of the structure, and it is convenient for the magnetic attraction component 6 to perform magnetic attraction and expulsion reactions on the magnetic beads in the mixed solution in the reaction tube 43 . In other embodiments, the magnetic rod assembly 61 can also move toward or away from the reaction tube 43 in other moving directions, such as horizontal running or tilting movement.
在一实施例中，磁棒组件61包括磁棒支架和磁棒611，磁棒611竖直设置且可拆卸地设置在磁棒支架上。通过设置磁棒611，能够使磁棒611插入试剂板4上的反应管43和相邻管结构之间，减小磁吸组件6与振荡加热组件3及试剂板4的干涉。在一实施例中，磁棒611沿Y方向并排设置有多个，且磁棒611的个数与位置与试剂板4的个数及反应管43的设置位置一一对应，且当磁棒611位于进行磁吸反应所在位置时，磁棒611与反应管加热块312上的管插孔319的开口位置正对。In one embodiment, the magnet bar assembly 61 includes a magnet bar bracket and a magnet bar 611, and the magnet bar 611 is vertically and detachably arranged on the magnet bar bracket. By arranging the magnetic bar 611 , the magnetic bar 611 can be inserted between the reaction tube 43 on the reagent plate 4 and the adjacent tube structures, thereby reducing the interference between the magnetic attraction component 6 , the oscillating heating component 3 and the reagent plate 4 . In one embodiment, a plurality of magnet bars 611 are arranged side by side along the Y direction, and the number and position of the magnet bars 611 correspond to the number of the reagent plates 4 and the setting positions of the reaction tubes 43 one-to-one, and when the magnet bars 611 When the magnet bar 611 is located at the position where the magnetic attraction reaction is performed, the opening position of the magnet bar 611 and the opening of the tube insertion hole 319 on the reaction tube heating block 312 are directly opposite.
磁棒支架包括沿Y方向设置的安装条612、水平设置且与安装条612可拆卸连接的安装横板613，安装横板613与磁棒驱动组件62连接。在一实施例中，安装条612向下凸设有定位部6123，安装横板613的上表面对应开设有定位槽6131，定位部6123插设在定位槽6131中，实现安装横板613与安装条612的安装定位。安装条612和安装横板613通过穿设于定位槽6131的槽底及安装部上的螺钉可拆 卸连接。The magnet bar support includes a mounting bar 612 disposed along the Y direction, a horizontal mounting plate 613 , which is detachably connected to the mounting bar 612 , and the mounting cross plate 613 is connected to the magnet bar driving assembly 62 . In one embodiment, the mounting bar 612 has a positioning portion 6123 protruding downward, and a positioning groove 6131 is correspondingly formed on the upper surface of the mounting cross plate 613. The positioning portion 6123 is inserted into the positioning groove 6131 to realize the installation and installation The installation positioning of the strip 612. The mounting bar 612 and the mounting transverse plate 613 are detachably connected through the bottom of the positioning groove 6131 and the screws on the mounting portion.
为方便磁棒611在安装条612上的设置，安装条612上沿竖直方向贯通有安装孔6121，磁棒611插入安装孔6121中并与安装孔6121过盈配合。为方便磁棒611的取放，磁棒611沿Z方向贯通开设有穿口6122，穿口6122呈长条状结构，且穿口6122的一端贯通安装条612的侧壁，穿口6122的另一端连通安装孔6121，穿口6122的开口宽度小于安装孔6121的直径。穿口6122的设置，能够使安装条612上形成有一端自由的安装臂结构，通过安装臂的弹性变形，使穿口6122在外力作用下张开，在去掉外力使闭合，从而使磁棒611能够通过穿口6122进入安装孔6121中。在一实施例中，安装条612由橡胶、塑料等弹性材料制成。In order to facilitate the arrangement of the magnet bar 611 on the mounting bar 612 , the mounting bar 612 has a mounting hole 6121 extending vertically through the mounting bar 612 . In order to facilitate the taking and placing of the magnet bar 611, the magnet bar 611 is provided with a through hole 6122 in the Z direction. One end communicates with the mounting hole 6121 , and the opening width of the through hole 6122 is smaller than the diameter of the mounting hole 6121 . The arrangement of the through-hole 6122 enables the mounting bar 612 to form a mounting arm structure with one end free. Through the elastic deformation of the mounting arm, the through-hole 6122 is opened under the action of external force, and closed when the external force is removed, so that the magnet bar 611 The mounting hole 6121 can be accessed through the through-hole 6122 . In one embodiment, the mounting bar 612 is made of elastic materials such as rubber and plastic.
在本实施例中，磁棒驱动组件62采用电机配合丝杆的驱动方式。在一实施例中，磁棒驱动组件62包括磁吸驱动电机、沿Z方向设置且下端与磁吸驱动电机的输出轴连接的磁吸丝杆及套设在磁吸丝杆上且与安装横板613连接的磁吸螺母座。在其他实施例中，也可以采用其他能够实现磁棒组件61竖直升降的磁吸驱动组件的结构，如采用直线电机、液压缸驱动等，此处不再一一赘述。In this embodiment, the magnetic rod driving assembly 62 adopts a driving method in which a motor cooperates with a screw rod. In one embodiment, the magnetic rod driving assembly 62 includes a magnetic driving motor, a magnetic screw rod arranged along the Z direction and connected to the output shaft of the magnetic driving motor at its lower end, and a magnetic screw rod sleeved on the magnetic suction rod and horizontal to the installation. The magnetic nut seat connected to the plate 613. In other embodiments, other structures of the magnetic drive assembly capable of vertically lifting and lowering the magnetic rod assembly 61 may also be used, such as using a linear motor, a hydraulic cylinder drive, etc., which will not be repeated here.
为对磁棒组件61的竖直运动进行导向，磁吸组件6还包括设置为沿竖直方向导向的磁吸导向组件63。为方便磁吸导向组件63和磁吸驱动电机的安装，磁吸组件6还包括与磁吸电机座64，磁吸导向组件63和磁吸驱动电机的固定端设置在磁吸电机座64上。磁吸电机座64的设置可参考上述盖板电机座53的设置，且磁吸导向组件63可参考盖板导向组件54的结构设置，此处不再赘述。且为提高导向稳定性，磁吸导向组件63沿Y方向间隔设置有两组，磁吸驱动组件位于两组磁吸导向组件63之间。In order to guide the vertical movement of the magnet bar assembly 61 , the magnetic attraction assembly 6 further includes a magnetic attraction guide assembly 63 arranged to be guided in the vertical direction. In order to facilitate the installation of the magnetic guide assembly 63 and the magnetic drive motor, the magnetic assembly 6 further includes a magnetic motor base 64 , and the fixed ends of the magnetic guide assembly 63 and the magnetic drive motor are arranged on the magnetic motor base 64 . The arrangement of the magnetic motor base 64 can refer to the above-mentioned arrangement of the cover motor base 53 , and the magnetic guide assembly 63 can refer to the structural arrangement of the cover guide assembly 54 , which will not be repeated here. In addition, in order to improve the guiding stability, two sets of magnetic guide assemblies 63 are arranged at intervals along the Y direction, and the magnetic drive assemblies are located between the two sets of magnetic guide assemblies 63 .
在一实施例中，为检测磁棒组件61的运行位移，磁吸组件6上设置有位移检测组件20。在本实施例中，位移检测组件20包括设置在安装横板613上的光电感应片202以及设置在磁吸电机座64上的光电开关201，光电开关201与光电感应片202正对设置。In one embodiment, in order to detect the running displacement of the magnet rod assembly 61 , the magnetic attraction assembly 6 is provided with a displacement detection assembly 20 . In this embodiment, the displacement detection assembly 20 includes a photoelectric induction sheet 202 arranged on the horizontal mounting plate 613 and a photoelectric switch 201 arranged on the magnetic motor base 64 .
如图2所示，第一移液机构7设置在沿安装架2长度方向的一端，且底板21上于沿试剂板4长度方向的一侧设置有试剂瓶存放区，试剂瓶存放区上设置有多个承载有杂交液、纯水等核酸检测和清洗所需液体的试剂瓶40。移液装置包括第一移液机构7及第二移液机构8，第一移液机构7设置为吸取所述试剂瓶40中的液体，第二移液机构8设置为取放试剂板4上的试剂。第一移液机构7设置在试剂存放区的上方，设置为抽取所述试剂瓶40中的液体；第二移液机构8设置为吸取和 转移所述试剂板4上的液体。As shown in FIG. 2 , the first pipetting mechanism 7 is arranged at one end along the length direction of the mounting frame 2 , and the bottom plate 21 is provided with a reagent bottle storage area at one side along the length direction of the reagent plate 4 , and the reagent bottle storage area is provided with a reagent bottle storage area. There are a plurality of reagent bottles 40 carrying liquids required for nucleic acid detection and cleaning, such as hybridization liquid and pure water. The pipetting device includes a first pipetting mechanism 7 and a second pipetting mechanism 8. The first pipetting mechanism 7 is configured to absorb the liquid in the reagent bottle 40, and the second pipetting mechanism 8 is configured to pick and place the reagent plate 4. reagent. The first pipetting mechanism 7 is arranged above the reagent storage area, and is configured to extract the liquid in the reagent bottle 40; the second pipetting mechanism 8 is configured to suck and transfer the liquid on the reagent plate 4.
如图2所示，在本实施例中，试剂瓶存放区沿Y方向并排设置有两列试剂瓶40，每列试剂瓶40包括沿X方向并排设置的五个试剂瓶40，每个试剂瓶40中可存放不同类型的液体。在其他实施例中，试剂瓶存放区中试剂瓶40的列数和每列中试剂瓶40的个数可以根据核酸检测的项目类型进行具体确定。As shown in FIG. 2 , in this embodiment, two rows of reagent bottles 40 are arranged side by side in the Y direction in the reagent bottle storage area, and each row of reagent bottles 40 includes five reagent bottles 40 arranged side by side along the X direction. Different types of liquids can be stored in 40. In other embodiments, the number of columns of reagent bottles 40 in the reagent bottle storage area and the number of reagent bottles 40 in each column may be specifically determined according to the type of nucleic acid detection items.
图17为本申请实施例提供的第一移液机构7在一个视角下的结构示意图，图18为本申请实施例提供的第一移液机构7在另一个视角下的结构示意图，图19为本申请实施例提供的第一移液机构7的拆分结构示意图，如图17-图19所示，第一移液机构7包括第一试剂针单元71、与第一试剂针单元71连接且设置为带动第一试剂针单元71竖直升降的第一竖直位移单元73和与第一竖直位移单元73连接且设置为带动第一试剂针单元71沿水平方向运动的第一水平位移单元72。FIG. 17 is a schematic structural diagram of the first pipetting mechanism 7 provided by the embodiment of the application from one perspective, FIG. 18 is a schematic structural diagram of the first pipetting mechanism 7 provided by the embodiment of the application from another perspective, and FIG. 19 is A schematic diagram of the split structure of the first pipetting mechanism 7 provided in the embodiment of the present application, as shown in FIGS. 17-19 , the first pipetting mechanism 7 includes a first reagent needle unit 71 , which is connected to the first reagent needle unit 71 and A first vertical displacement unit 73 configured to drive the first reagent needle unit 71 to rise and fall vertically and a first horizontal displacement unit connected to the first vertical displacement unit 73 and configured to drive the first reagent needle unit 71 to move in the horizontal direction 72.
第一水平位移单元72包括连接件722及第一水平驱动组件721，连接件722与安装架2的横梁221连接，第一水平驱动组件721的固定端设置在连接件722上，第一水平驱动组件721的驱动端与第一竖直位移单元73连接。在一实施例中，第一水平驱动组件721采用电机配合丝杆的驱动方式，第一水平驱动组件721包括水平驱动电机、沿水平方向设置且一端与水平驱动电机的输出轴连接的水平丝杆、套设在水平丝杆上的水平螺母座，水平螺母座与竖直驱动组件连接。水平驱动电机通过电机固定板723固定于板状的连接件722上。在本实施例中，水平丝杆沿Y方向设置，以驱动第一试剂针单元71沿Y方向运动，在其他实施例中，水平丝杆也可以沿X方向设置，以驱动第一试剂针单元71沿X方向运动。The first horizontal displacement unit 72 includes a connecting piece 722 and a first horizontal driving component 721. The connecting piece 722 is connected with the beam 221 of the mounting frame 2, and the fixed end of the first horizontal driving component 721 is set on the connecting piece 722. The driving end of the assembly 721 is connected with the first vertical displacement unit 73 . In one embodiment, the first horizontal drive assembly 721 adopts a driving method in which a motor cooperates with a screw rod. The first horizontal drive assembly 721 includes a horizontal drive motor, a horizontal screw rod arranged in a horizontal direction and one end of which is connected to the output shaft of the horizontal drive motor. , a horizontal nut seat sleeved on the horizontal screw rod, and the horizontal nut seat is connected with the vertical drive assembly. The horizontal drive motor is fixed on the plate-shaped connecting member 722 through the motor fixing plate 723 . In this embodiment, the horizontal screw rod is set along the Y direction to drive the first reagent needle unit 71 to move along the Y direction. In other embodiments, the horizontal screw rod can also be set along the X direction to drive the first reagent needle unit 71 71 moves in the X direction.
第一竖直位移单元73包括沿X方向水平设置的转接板731以及设置在转接板731上的第一竖直驱动组件732，第一竖直驱动组件732包括固定在转接板731上的驱动电机、竖直设置且一端与驱动电机连接的丝杆、套设在丝杆上的螺母座，螺母座与第一试剂针单元71连接。The first vertical displacement unit 73 includes an adapter plate 731 arranged horizontally along the X direction and a first vertical drive assembly 732 disposed on the adapter plate 731 , and the first vertical drive assembly 732 includes a first vertical drive assembly 732 fixed on the adapter plate 731 A driving motor, a screw rod arranged vertically and one end connected to the driving motor, a nut seat sleeved on the screw rod, and the nut seat is connected with the first reagent needle unit 71 .
为实现对第一水平位移单元72和第一竖直位移单元73的导向，第一移液机构7还包括第一水平导向组件74和第一竖直导向组件75，第一水平导向组件74设置在连接件722上，第一竖直导向组件75设置在与转接板731连接且竖直设置的导轨板77上。第一水平导向组件74和第一竖直导向组件75采用导轨滑块的结构形式，且采用导轨滑块的结构实现直线导向的设置为本领域的常规设置，此处不再赘述。且为了实现第一水平驱动组件721和第一竖直驱动组件732的移动限位和缓冲，每个导轨的两个末端均连接有缓冲块76。In order to guide the first horizontal displacement unit 72 and the first vertical displacement unit 73, the first pipetting mechanism 7 further includes a first horizontal guide assembly 74 and a first vertical guide assembly 75, and the first horizontal guide assembly 74 is provided with On the connecting piece 722, the first vertical guide assembly 75 is arranged on the guide rail plate 77 which is connected with the adapter plate 731 and is arranged vertically. The first horizontal guide assembly 74 and the first vertical guide assembly 75 are in the form of guide rail sliders, and the configuration of using the guide rail slider structure to achieve linear guidance is a conventional configuration in the field, and will not be repeated here. And in order to realize the movement limit and buffering of the first horizontal driving component 721 and the first vertical driving component 732 , both ends of each guide rail are connected with buffer blocks 76 .
第一试剂针单元71包括水平设置的第一试剂针架712和设置在第一试剂针架712上的第一试剂针711。在本实施例中，第一试剂针架712沿X方向设置且一端与第一竖直导向组件75的滑块连接。第一试剂针711竖直设置，且第一试剂针711沿X方向间隔设置有多个。第一试剂针711的个数和位置与试剂瓶存放区中每列试剂瓶中的试剂瓶40的个数和位置一一对应。The first reagent needle unit 71 includes a first reagent needle holder 712 arranged horizontally and a first reagent needle 711 arranged on the first reagent needle holder 712 . In this embodiment, the first reagent needle rack 712 is disposed along the X direction and one end is connected to the slider of the first vertical guide assembly 75 . The first reagent needles 711 are arranged vertically, and a plurality of first reagent needles 711 are arranged at intervals along the X direction. The number and position of the first reagent needles 711 correspond to the number and position of the reagent bottles 40 in each row of reagent bottles in the reagent bottle storage area on a one-to-one basis.
在本实施例中，第一试剂针711与第一试剂针架712插接，第一试剂针711与第一试剂针架712的连接方式还可参考上述的磁棒611与安装条612的安装方式，此处不再赘述。In this embodiment, the first reagent needle 711 is plugged into the first reagent needle rack 712 , and the connection between the first reagent needle 711 and the first reagent needle rack 712 can also refer to the above-mentioned installation of the magnet bar 611 and the mounting bar 612 method, which will not be repeated here.
在一实施例中，为检测第一水平位移单元72和第一竖直位移单元73的位移，第一移液机构7还包括第一水平位移检测组件和第一位移检测组件，且第一水平位移检测组件和第一竖直位移检测组件采用光电开关201配合光电感应片202的检测形式，此处不再赘述。In one embodiment, in order to detect the displacement of the first horizontal displacement unit 72 and the first vertical displacement unit 73, the first pipetting mechanism 7 further includes a first horizontal displacement detection component and a first displacement detection component, and the first horizontal displacement The displacement detection component and the first vertical displacement detection component adopt the detection form in which the photoelectric switch 201 cooperates with the photoelectric sensor sheet 202 , and details are not described herein again.
图20为本申请实施例提供的第二移液机构在一个视角下的结构示意图，图21为本申请提供的第二移液机构在另一个视角下的结构示意图，图22为本申请实施例提供的第二移液机构的拆分结构示意图，如图20-图22所示，第二移液机构8包括设置为移液的第二试剂针单元81、设置为驱动第二试剂针单元81竖直升降的第二竖直位移单元82、设置为更换和插取吸头的吸头取放单元83、设置为驱动吸头取放单元83竖直升降的第三竖直位移单元84以及设置为驱动第二竖直位移单元82和第三竖直位移单元84沿Y方向移动的第二水平移动单元。该种设置形式，能够提高第二移液机构8的结构紧凑性。FIG. 20 is a schematic structural diagram of the second liquid pipetting mechanism provided by the embodiment of the application from one perspective, FIG. 21 is a schematic structural diagram of the second liquid pipetting mechanism provided by the application from another perspective, and FIG. 22 is an embodiment of the application. 20-22, the second pipetting mechanism 8 includes a second reagent needle unit 81 configured to pipette liquid, and a second reagent needle unit 81 configured to drive the liquid. The second vertical displacement unit 82 vertically lifted, the tip pick-and-place unit 83 configured to replace and insert the tip, the third vertical displacement unit 84 configured to drive the vertical lift of the tip pick-and-place unit 83, and the A second horizontal moving unit for driving the second vertical displacement unit 82 and the third vertical displacement unit 84 to move in the Y direction. This arrangement can improve the compactness of the structure of the second pipetting mechanism 8 .
第二水平移动单元包括与XZ平面平行的固定立板85及第二水平驱动单元(未示出)，第二水平驱动单元的固定端与横梁221连接，第二水平驱动单元的驱动端与固定立板85连接，设置为驱动连接立板沿Y方向往复运动。第二水平驱动单元可以采用电机配合丝杆螺母的结构形式，也可以采用电机配合链轮链条的结构形式，或可以采用其他能够实现固定立板85的水平运动的结构形成，此处不再赘述。The second horizontal moving unit includes a fixed vertical plate 85 parallel to the XZ plane and a second horizontal driving unit (not shown). The fixed end of the second horizontal driving unit is connected to the beam 221 , and the driving end of the second horizontal driving unit is connected to the fixed The vertical plates 85 are connected and arranged to drive the connecting vertical plates to reciprocate along the Y direction. The second horizontal driving unit can be in the form of a motor matching a screw nut, or a motor matching a sprocket chain, or other structures that can realize the horizontal movement of the fixed vertical plate 85 , which will not be repeated here. .
第二竖直位移单元82和第三竖直位移单元84设置在固定立板85的同一面。在一实施例中，固定立板85连接有与固定立板85垂直的水平设置的固定横板86，第二竖直位移单元82和第三竖直位移单元84的固定端连接于固定横板86上，且第二竖直位移单元82和第三竖直位移单元84沿X方向并排设置。在本实施例中，第二竖直位移单元82和第三竖直位移单元84采用电机配合丝杆螺母的驱动形式， 结构简单，连接方便。在其他实施例中，第二竖直位移单元82和第三竖直位移单元84还可以采用电机配合齿轮齿条等的结构形式，或采用直线电机、液压缸等驱动形式。且可以实现竖直方向的直线驱动的结构形式为本领域的常规设置，此处不再赘述。The second vertical displacement unit 82 and the third vertical displacement unit 84 are arranged on the same surface of the fixed vertical plate 85 . In one embodiment, the fixed vertical plate 85 is connected with a horizontal fixed horizontal plate 86 perpendicular to the fixed vertical plate 85 , and the fixed ends of the second vertical displacement unit 82 and the third vertical displacement unit 84 are connected to the fixed horizontal plate. 86, and the second vertical displacement unit 82 and the third vertical displacement unit 84 are arranged side by side along the X direction. In this embodiment, the second vertical displacement unit 82 and the third vertical displacement unit 84 are driven by a motor and a screw nut, and have a simple structure and convenient connection. In other embodiments, the second vertical displacement unit 82 and the third vertical displacement unit 84 may also adopt a structural form in which a motor cooperates with a rack and pinion, or a driving form such as a linear motor, a hydraulic cylinder, or the like. And the structural form that can realize the linear drive in the vertical direction is a conventional setting in the field, and will not be repeated here.
吸头取放单元83包括吸头座831、竖直设置在吸头座831上的吸头套杆832，穿设于吸头套杆832内部的吸头针833以及连接于吸头套杆832下端的吸头嘴834，吸头针833的下端密封插设于吸头嘴834内。吸头座831与对应的螺母座可拆卸连接。在本实施例中，吸头套杆832为由不锈钢制成的空心杆状结构，吸头嘴834的上端过盈插设于吸头套杆832下端内。吸头针833上端伸出吸头套杆832的外部，方便与进液管702的连接。The tip pick-and-place unit 83 includes a tip seat 831 , a tip cover rod 832 vertically arranged on the tip cover 831 , a tip needle 833 passing through the inside of the tip cover rod 832 , and a suction tip connected to the lower end of the tip cover rod 832 . The tip nozzle 834, the lower end of the tip needle 833 is sealed and inserted into the tip nozzle 834. The suction head seat 831 is detachably connected with the corresponding nut seat. In this embodiment, the suction head cover rod 832 is a hollow rod-shaped structure made of stainless steel, and the upper end of the suction head nozzle 834 is inserted into the lower end of the suction head cover rod 832 by interference. The upper end of the tip needle 833 protrudes from the outside of the tip cover rod 832 to facilitate connection with the liquid inlet tube 702 .
在本实施例中，吸头座831与吸头套杆832的安装方式可参考上述的磁棒611与安装条612的安装方式，此处不再赘述。在本实施例中，吸头套杆832沿Y方向间隔设置有多个，以实现对多个吸头的吸取和更换。吸头套杆832的个数与核酸检测仪单次能够同步进行的核酸检测个数相同，可以但不限定为4个。In the present embodiment, the installation method of the suction head seat 831 and the suction head sleeve rod 832 can refer to the above-mentioned installation method of the magnetic rod 611 and the installation strip 612 , which will not be repeated here. In this embodiment, a plurality of suction head cover rods 832 are provided at intervals along the Y direction, so as to realize suction and replacement of multiple suction heads. The number of the tip cover rods 832 is the same as the number of nucleic acid detections that the nucleic acid detector can perform synchronously at one time, and may be, but is not limited to, four.
吸头嘴834设置为插取位于试剂板4上吸头腔414中的吸头，为方便退掉用过的吸头，第二移液机构8还包括设置为退掉吸头的退吸头板87。退吸头板87与固定立板85可拆卸连接，且退吸头板87上开设有退吸头孔871。吸头套杆832的下端插设于退吸头孔871中，且退吸头孔871的内径大于吸头嘴834的最大外径且小于吸头的最大外径。该种设置方式，当第三竖直位移单元84带动插设有吸头的吸头套杆832向上运动时，吸头在退吸头板87的阻碍作用下相对吸头嘴834分离，实现吸头的退去操作。The tip nozzle 834 is configured to insert and remove the tip located in the tip chamber 414 on the reagent plate 4. In order to facilitate the withdrawal of the used tip, the second pipetting mechanism 8 also includes a tip ejection set for ejecting the tip. plate 87. The suction head plate 87 is detachably connected to the fixed vertical plate 85 , and the suction head plate 87 is provided with a suction head hole 871 . The lower end of the tip cover rod 832 is inserted into the tip ejection hole 871 , and the inner diameter of the tip ejection hole 871 is larger than the maximum outer diameter of the tip nozzle 834 and smaller than the maximum outer diameter of the tip. In this arrangement, when the third vertical displacement unit 84 drives the suction head sleeve 832 with the suction head inserted to move upward, the suction head is separated from the suction head nozzle 834 under the obstruction of the suction head ejection plate 87, so that the suction head is realized. the retreat operation.
第二试剂针单元81包括针架811、废液针812及试剂针束813，针架811与对应的螺母座可拆卸连接，且废液针812和试剂针束813可拆卸地设置在针架811上。在本实施例中，废液针812和试剂针束813沿X方向并排且间隔设置，且通过设计专用的废液针812排出废液，能够减少试剂之间的污染。在本实施例中，废液针812与试剂针束813沿Y方向平行且间隔设置有多个，且废液针812及试剂针束813的个数与核酸检测仪单次同时能够进行的核酸检测数量相同。The second reagent needle unit 81 includes a needle holder 811, a waste needle 812 and a reagent needle bundle 813. The needle holder 811 is detachably connected to the corresponding nut seat, and the waste needle 812 and the reagent needle bundle 813 are detachably arranged on the needle holder. 811 on. In this embodiment, the waste liquid needle 812 and the reagent needle bundle 813 are arranged side by side along the X direction and arranged at intervals, and the waste liquid is discharged by designing a dedicated waste liquid needle 812, which can reduce the contamination between the reagents. In this embodiment, a plurality of waste liquid needles 812 and reagent needle bundles 813 are arranged parallel to and spaced in the Y direction, and the number of waste liquid needles 812 and reagent needle bundles 813 is the same as the nucleic acid that the nucleic acid detector can perform at the same time at a time. The number of detections is the same.
在本实施例中，针架811包括针固定座8111、支撑杆8112及针安装座8113，针固定座8111与对应的螺母座连接，支撑杆8112上端与针固定座8111可拆卸连接，支撑杆8112下端与针安装座8113可拆卸连接。该种设置方式，能够在减轻针架811的整体重量的基础上，保证针架811设置的稳定性，缩短试剂针束813及 废液针812需要竖直升降的所需行程。In this embodiment, the needle holder 811 includes a needle holder 8111, a support rod 8112 and a needle holder 8113. The needle holder 8111 is connected to the corresponding nut holder, the upper end of the support rod 8112 is detachably connected to the needle holder 8111, and the support rod The lower end of 8112 is detachably connected to the needle mount 8113. This setting method can reduce the overall weight of the needle holder 811, ensure the stability of the needle holder 811, and shorten the required stroke of the reagent needle bundle 813 and the waste needle 812 to be vertically lifted.
在一实施例中，试剂针束813包括针束套管8132及穿设于针束套管8132内的多根第二试剂针8131，针束套管8132及废液针812与针安装座8113的连接方式可参考上述磁棒611与安装条612的安装方式，此处不再赘述。在本实施例中，针束套管8132内的第二试剂针8131有五根，且每个针束套管8132内的第二试剂针8131的根数与第一移液机构7中第一试剂针711的根数相同。In one embodiment, the reagent needle bundle 813 includes a needle bundle cannula 8132 and a plurality of second reagent needles 8131 passing through the needle bundle cannula 8132 , the needle bundle cannula 8132 , the waste liquid needle 812 and the needle mounting seat 8113 For the connection method, please refer to the above-mentioned installation method of the magnet bar 611 and the mounting bar 612, which will not be repeated here. In this embodiment, there are five second reagent needles 8131 in the needle bundle cannula 8132 , and the number of the second reagent needles 8131 in each needle bundle cannula 8132 is the same as the number of the second reagent needles 8131 in the first pipetting mechanism 7 . The number of reagent needles 711 is the same.
为防止液体通过第二试剂针8131下端流出的流速过快造成溶液飞溅，第二试剂针8131包括竖直设置的主体部81311和与主体部81311连接并与主体部81311呈非零夹角的导向部81312，主体部81311与导向部81312一体成型，导向部81312相对主体部81311的中心轴线向外倾斜，在一实施例中，导向部81312与主体部81311之间的夹角为5°-10°。在其他实施例中，同一试剂针束813内的第二试剂针8131末端倾斜方向不同。In order to prevent the liquid flowing out through the lower end of the second reagent needle 8131 from being too fast to cause the solution to splash, the second reagent needle 8131 includes a vertically arranged main body portion 81311 and a guide connected to the main body portion 81311 and forming a non-zero included angle with the main body portion 81311 part 81312, the main body part 81311 and the guide part 81312 are integrally formed, and the guide part 81312 is inclined outward relative to the central axis of the main body part 81311. In one embodiment, the included angle between the guide part 81312 and the main body part 81311 is 5°-10° °. In other embodiments, the inclination directions of the ends of the second reagent needles 8131 in the same reagent needle bundle 813 are different.
为实现第二竖直位移单元82和第三竖直位移单元84的运动导向，第二移液机构8还包括第二竖直导向组件88和第三竖直导向组件89，第二竖直导向组件88和第三竖直导向组件89沿Y方向并排设置，且第二竖直导向组件88和第三竖直导向组件89采用导轨滑块的结构形式，吸头座831及针固定座8111分别与对应的竖直导向组件的滑块连接。In order to realize the motion guidance of the second vertical displacement unit 82 and the third vertical displacement unit 84, the second pipetting mechanism 8 further includes a second vertical guide assembly 88 and a third vertical guide assembly 89. The second vertical guide The assembly 88 and the third vertical guide assembly 89 are arranged side by side along the Y direction, and the second vertical guide assembly 88 and the third vertical guide assembly 89 are in the form of guide rail sliders, and the suction head seat 831 and the needle fixing seat 8111 are respectively Connect with the slider of the corresponding vertical guide assembly.
在本实施例中，为方便检测吸头取放组件及第二试剂针单元81的位移，第二移液机构8还包括分别设置为检测第二试剂针单元81竖直升降位移和吸头取放组件的竖直升降位移的位移检测组件20。在本实施例中，位移检测组件20采用光电开关201配合光电感应片202的结构形式，在其他实施例中，也可以采用其他能够实现位移检测的检测设备。In this embodiment, in order to facilitate the detection of the displacement of the pick-and-place assembly of the tip and the second reagent needle unit 81, the second pipetting mechanism 8 further includes a device configured to detect the vertical displacement of the second reagent needle unit 81 and the displacement of the tip pick-up unit 81, respectively. The displacement detection assembly 20 for the vertical lifting displacement of the placing assembly. In this embodiment, the displacement detection assembly 20 adopts a structure in which the photoelectric switch 201 cooperates with the photoelectric induction sheet 202. In other embodiments, other detection devices capable of realizing displacement detection may also be used.
为方便组装和拆卸，固定立板85包括可拆卸的门架部851和立板部852，门架部851呈下端开口的U型结构，立板部852竖直设置且与门架部851的两侧可拆卸连接，固定横板86设置在门架部851上，两个竖直导向组件均设置在立板部852上。In order to facilitate assembly and disassembly, the fixed vertical plate 85 includes a detachable gantry portion 851 and a vertical plate portion 852. The gantry portion 851 has a U-shaped structure with an open lower end. The two sides are detachably connected, the fixed horizontal plate 86 is arranged on the gantry portion 851 , and the two vertical guide assemblies are both arranged on the vertical plate portion 852 .
为了使试剂针对液体进行吸取，移液机构还包括管路组件70及泵单元，管路组件70组成移液所需管路，泵组件为液体的取放提供动力。在本实施例中，管路组件70包括吸头管路组件和试剂针管路组件，泵单元包括注射泵10和液泵组9，注射泵10通过吸头管路组件与吸头取放单元83连接，液泵组9通过试剂针管路组件与第一试剂针单元71及第二试剂针单元81连接。In order to make the reagent suck the liquid, the pipetting mechanism further includes a pipeline assembly 70 and a pump unit. The pipeline assembly 70 constitutes a pipeline required for pipetting, and the pump assembly provides power for picking and placing the liquid. In this embodiment, the pipeline assembly 70 includes a pipette pipe assembly and a reagent needle pipe assembly, the pump unit includes a syringe pump 10 and a liquid pump group 9, and the syringe pump 10 communicates with the pipette tip pick-and-place unit 83 through the pipette pipe assembly Connection, the liquid pump group 9 is connected to the first reagent needle unit 71 and the second reagent needle unit 81 through the reagent needle pipeline assembly.
具体地，图23为本申请实施例提供的吸头管路组件、注射泵10及吸头取放单元83的管路连接示意图，如23图所示，吸头管路组件包括多个吹吸管701，吹吸管701的个数与吸头针833的个数相同，且吹吸管701的一端与吸头针833的上端密封连通，吹吸管701的另一端与注射泵10连接。吸头管路组件和注射泵10的设置，能够在核酸提取过程中对反应液进行移液和吹打混匀。Specifically, FIG. 23 is a schematic diagram of the pipeline connections of the suction head pipeline assembly, the syringe pump 10 and the suction head pick-and-place unit 83 provided by the embodiment of the application. As shown in FIG. 23 , the suction head pipeline assembly includes a plurality of suction pipes 701 , the number of suction pipes 701 is the same as the number of suction needles 833 , one end of the suction pipe 701 is in sealing communication with the upper end of the suction needle 833 , and the other end of the suction pipe 701 is connected to the syringe pump 10 . The arrangement of the pipette pipe assembly and the syringe pump 10 enables pipetting, pipetting and mixing of the reaction solution during the nucleic acid extraction process.
图24为本申请实施例提供液泵及试剂针管路组件70的连接关系示意图，如图24所示，液泵组9具有多对试剂进液口和试剂出液口，试剂针管路包括连接于液泵组9和第一试剂针711之间的进液管702，进液管702的第一端与第一试剂针711的上端密封连通，进液管702的第二端与液泵组9的试剂进液口连通。且进液管702的个数与第一试剂针711的个数相同，每个进液管702连通于不同的试剂进液口。试剂针管路组件还包括主出液管703，主出液管703的第一端连接于与上述进液管702对应的试剂出液口处，每个主出液管703的第二端连通多个分出液管704的第一端，多个分出液管704的第二端分别连通多个试剂针束813中的其中一个第二试剂针8131。如在本实施例中，试剂针束813有四组，每个主出液管703均连通有四个分出液管704。FIG. 24 is a schematic diagram of the connection relationship between the liquid pump and the reagent needle pipeline assembly 70 according to the embodiment of the application. As shown in FIG. 24 , the liquid pump set 9 has multiple pairs of reagent liquid inlets and reagent liquid outlets, and the reagent needle pipeline includes a The liquid inlet pipe 702 between the liquid pump group 9 and the first reagent needle 711, the first end of the liquid inlet pipe 702 is in sealing communication with the upper end of the first reagent needle 711, and the second end of the liquid inlet pipe 702 is connected to the liquid pump group 9. connected to the reagent inlet. Moreover, the number of the liquid inlet pipes 702 is the same as the number of the first reagent needles 711 , and each liquid inlet pipe 702 is connected to a different reagent liquid inlet port. The reagent needle pipeline assembly also includes a main liquid outlet pipe 703. The first end of the main liquid outlet pipe 703 is connected to the reagent liquid outlet corresponding to the above-mentioned liquid inlet pipe 702, and the second end of each main liquid outlet pipe 703 is connected to many The first ends of the aliquots 704 and the second ends of the plurality of aliquots 704 are respectively connected to one of the second reagent needles 8131 in the plurality of reagent needle bundles 813 . As in this embodiment, the reagent needle bundle 813 has four groups, and each main liquid outlet pipe 703 is connected with four branch liquid pipes 704 .
试剂针管路组件还包括主废液进管706和多个分废液进管707，分废液进管707的个数与废液针812的个数相同，且每个分废液进管707的第一端连通一废液针812的上端，分废液进管707的第二端连通主废液进管706的第一端，主废液进管706的第二端连通液泵组9中的废液进液口，与废液进液口相对应的废液出液口连通废液出管707的第一端，废液出管707的第二端连通废液瓶80。在本实施例中，废液瓶80由塑料材料制成，且里面可盛放氯酸钠等消毒试剂，用于废液的排出和核酸产物的消化。The reagent needle pipeline assembly also includes a main waste liquid inlet pipe 706 and a plurality of waste liquid inlet pipes 707, the number of the waste liquid inlet pipes 707 is the same as the number of waste liquid needles 812, and each waste liquid inlet pipe 707 The first end of the waste liquid is connected to the upper end of a waste liquid needle 812, the second end of the waste liquid inlet pipe 707 is connected to the first end of the main waste liquid inlet pipe 706, and the second end of the main waste liquid inlet pipe 706 is connected to the liquid pump group 9 The waste liquid inlet port in , the waste liquid outlet port corresponding to the waste liquid inlet port is connected to the first end of the waste liquid outlet pipe 707 , and the second end of the waste liquid outlet pipe 707 is connected to the waste liquid bottle 80 . In this embodiment, the waste liquid bottle 80 is made of a plastic material, and can contain a disinfectant such as sodium chlorate, which is used for the discharge of waste liquid and the digestion of nucleic acid products.
在本实施例中，由于第一试剂针711对位于试剂瓶存放区中的试剂瓶40中的液体进行抽取，为检测试剂瓶40中的液位是否满足使用需求，试剂瓶存放区还设置有液位感应组件30，设置为检测每个试剂瓶40中的液位。液位感应组件30可参考相关技术中的产品，此处不再赘述。在一实施例中，试剂瓶40设置在液位感应组件30上，且液位感应组件30对应每个试剂瓶40设置有加热块和散热器，对试剂瓶40中的试剂的加热或散热。In this embodiment, since the first reagent needle 711 extracts the liquid in the reagent bottle 40 located in the reagent bottle storage area, in order to detect whether the liquid level in the reagent bottle 40 meets the usage requirements, the reagent bottle storage area is further provided with a The liquid level sensing assembly 30 is configured to detect the liquid level in each reagent bottle 40 . For the liquid level sensing assembly 30, reference may be made to products in the related art, and details are not described herein again. In one embodiment, the reagent bottle 40 is disposed on the liquid level sensing assembly 30 , and the liquid level sensing assembly 30 is provided with a heating block and a radiator corresponding to each reagent bottle 40 to heat or dissipate the reagent in the reagent bottle 40 .
在本实施例中，为了保证核酸检测仪内部的散热和通风，外壳1内部设置有抽风过滤系统，抽风过滤系统能够促进容纳空间中的空气流动，带动热量及时有效散出，且能够对空气进行过滤，减少试剂板4上的试剂和样本的污染，同时 也能防止核酸检测仪内部的样本和试剂之间的交叉污染。且抽风过滤系统具备核酸过滤功能，防止对外部环境造成污染抽风过滤系统的设置可参考现有的抽风过滤系统的设置，此处不再赘述。In this embodiment, in order to ensure the heat dissipation and ventilation inside the nucleic acid detector, the casing 1 is provided with an air extraction filter system, which can promote the air flow in the accommodation space, drive the heat to be dissipated in time and effectively, and can carry out Filtration can reduce the contamination of reagents and samples on the reagent plate 4, and can also prevent cross-contamination between samples and reagents in the nucleic acid detector. In addition, the ventilation filtration system has a nucleic acid filtering function to prevent pollution to the external environment. The settings of the ventilation filtration system can refer to the settings of the existing ventilation filtration system, which will not be repeated here.
在一实施例中，外壳1内部还设置有杀菌消毒系统，杀菌消毒系统对外壳1内部的设备进行消毒和杀菌，进一步地减少核酸污染，降低对工作人员的影响。在本实施例中，杀菌消毒系统采用紫外杀菌，但可以理解的是，还可以采用其他能够进行杀菌消毒的方式。In one embodiment, a sterilization and disinfection system is also provided inside the casing 1, and the sterilization and disinfection system sterilizes and sterilizes the equipment inside the casing 1, further reducing nucleic acid pollution and reducing the impact on the staff. In this embodiment, the sterilization and disinfection system adopts ultraviolet sterilization, but it can be understood that other methods capable of sterilization and disinfection may also be used.
本申请提供的核酸检测仪，将生物样本核酸提取和纯化、核酸扩增和分子杂交检测集中于一台仪器上，操作人员只需进行简单的准备动作即可在3.5h-6h内完成全部检测过程，得出结果，即，操作人员只需进行放一次性试剂板4、放扩增管、放杂交试剂、加入样本及启动仪器。核酸检测仪本处理量大，一次可处理24个样本，整个过程高效、便捷，适用于临床检测。The nucleic acid detector provided in this application integrates nucleic acid extraction and purification, nucleic acid amplification and molecular hybridization detection of biological samples on one instrument. The operator only needs to perform simple preparations to complete all detection within 3.5h-6h The result is obtained, that is, the operator only needs to put the disposable reagent plate 4, put the amplification tube, put the hybridization reagent, add the sample and start the instrument. The nucleic acid detector has a large processing capacity and can process 24 samples at a time. The whole process is efficient and convenient, and is suitable for clinical testing.
实施例二 Embodiment 2
本实施例提供了一种核酸检测方法，应用于实施例一中的核酸检测仪，用于核酸检测项目的高效自动化进行。This embodiment provides a nucleic acid detection method, which is applied to the nucleic acid detector in the first embodiment, and is used for efficient and automated nucleic acid detection items.
核酸检测方法包括前期准备、核酸提取和纯化、核酸扩增反应及核酸杂交反应五个大。Nucleic acid detection methods include five majors: preliminary preparation, nucleic acid extraction and purification, nucleic acid amplification reaction and nucleic acid hybridization reaction.
在一实施例中，前期准备包括：In one embodiment, the preliminary preparations include:
S101、通过第二前门13，在试剂瓶存放区中放置核酸检测用的纯化水和杂交液；S101, through the second front door 13, place purified water and hybridization solution for nucleic acid detection in the reagent bottle storage area;
S102中，取出密封的试剂板4，并撕掉塑封膜，在样本管44中加入待检测的样本，将核酸扩增管42插入扩增孔位411中，并打开核酸扩增管盖，将核酸扩增管盖卡入至卡口413中；In S102, take out the sealed reagent plate 4, tear off the plastic sealing film, add the sample to be detected in the sample tube 44, insert the nucleic acid amplification tube 42 into the amplification hole 411, open the nucleic acid amplification tube cover, and put the The nucleic acid amplification tube cover is snapped into the bayonet 413;
S103中，通过第一前门12将试剂板4放入至耗材面板50中，并封闭外壳1。In S103, the reagent plate 4 is put into the consumables panel 50 through the first front door 12, and the casing 1 is closed.
S104中，通过显示屏60选择并启动待进行的核酸检测项目。In S104, the nucleic acid detection item to be performed is selected and activated through the display screen 60.
S101-S104为手动进行的操作。S101-S104 are operations performed manually.
S105中，盖板组件5向下运动封闭核酸核酸扩增管42的管口。In S105, the cover plate assembly 5 moves downward to close the nozzle of the nucleic acid amplification tube 42.
S106中，液泵组9通过第一试剂针711抽取杂交液填充管路。In S106, the liquid pump group 9 draws the hybridization liquid through the first reagent needle 711 to fill the pipeline.
S107中，抽风过滤系统启动运行。In S107, the ventilation filtration system starts to operate.
S105-S107中，为核酸检测仪的自动化运行，且S105、S106和S107可同步进行，不区分先后。In S105-S107, it is the automatic operation of the nucleic acid detector, and S105, S106 and S107 can be performed synchronously, without distinction.
基于磁珠法进行核酸提取和纯化，包括：Magnetic bead-based nucleic acid extraction and purification, including:
S201中，第二移液机构8运动，使吸头嘴834运行至吸头腔414插取吸头。In S201, the second pipetting mechanism 8 moves, so that the tip nozzle 834 runs to the tip chamber 414 to insert the tip.
S202中，第二移液机构8运动，从样本管44中取样本液体加入到含有蛋白酶K和磁珠的试剂管42中。In S202, the second pipetting mechanism 8 moves, and the sample liquid is taken from the sample tube 44 and added to the reagent tube 42 containing proteinase K and magnetic beads.
S203中，注射泵10动作，使吸头反复吸取和放开上述试剂管42中的液体，以吹打液体至少一次，以使试剂管42中的磁珠打散。In S203, the syringe pump 10 is actuated, so that the suction head repeatedly sucks and releases the liquid in the reagent tube 42, so as to blow the liquid at least once, so as to disperse the magnetic beads in the reagent tube 42.
S204中，第二移液机构8动作，吸头抽取混匀后的蛋白酶K、磁珠和样本混合液至含有磁珠结合液的反应管43中。In S204, the second pipetting mechanism 8 is actuated, and the pipette tip extracts the mixed solution of proteinase K, magnetic beads and sample into the reaction tube 43 containing the magnetic bead binding solution.
S205中，反应管加热块312对应的加热膜315通电工作，对反应管43进行加热，同时，注射泵10动作，反复吹打反应管43中的液体至少一次；在此过程中，样本释放出核酸以及蛋白质等杂质，核酸吸附在磁珠上。In S205, the heating film 315 corresponding to the reaction tube heating block 312 is energized and operated to heat the reaction tube 43. At the same time, the syringe pump 10 operates to repeatedly blow the liquid in the reaction tube 43 at least once; during this process, the sample releases nucleic acid As well as impurities such as proteins, nucleic acids are adsorbed on the magnetic beads.
S206中，磁吸组件6动作，使磁棒611升高运动至反应管43的一侧中部进行吸磁；通过S206将核酸集中在一个区域中。In S206, the magnetic attraction component 6 is actuated, so that the magnetic rod 611 is moved up and moved to the middle of one side of the reaction tube 43 to attract magnetism; the nucleic acid is concentrated in one area through S206.
S207中，第二移液机构8及注射泵10动作，将反应管43中的液体吸取至废液槽415中；由于核酸已经集中在一个区域，抽吸混合液的时候可以避免将核酸祛除，从而最大限度地将核酸保存下来，提高核酸浓度测量的精度。In S207, the second pipetting mechanism 8 and the syringe pump 10 act to suck the liquid in the reaction tube 43 into the waste liquid tank 415; since the nucleic acid has been concentrated in one area, the nucleic acid can be avoided when the mixed liquid is sucked. Thereby, the nucleic acid can be preserved to the maximum extent and the accuracy of nucleic acid concentration measurement can be improved.
重复执行S207至少一次。Repeat S207 at least once.
S208中，第二移液机构8及注射泵10动作，从含洗涤液的试剂管42中吸取液体到反应管43中，且磁吸组件6动作，使磁棒611向下运动至远离反应管43。In S208, the second pipetting mechanism 8 and the syringe pump 10 act to suck the liquid from the reagent tube 42 containing the washing solution into the reaction tube 43, and the magnetic suction assembly 6 acts to move the magnetic rod 611 downward to away from the reaction tube 43.
S209中，注射泵10动作，通过吸头反复吸取和放开反应管43中的液体，实现对反应管43中的液体的反复吹打；通过S209对磁珠上的核酸进行洗涤，提高核酸的纯度。In S209, the syringe pump 10 is actuated, and the liquid in the reaction tube 43 is repeatedly sucked and released by the suction head to realize repeated blowing and blowing of the liquid in the reaction tube 43; the nucleic acid on the magnetic beads is washed in S209 to improve the purity of the nucleic acid .
S210中，磁吸组件6动作，使磁棒611升高运动至反应管43的一侧中部进行吸磁。In S210, the magnetic attraction component 6 is actuated, so that the magnetic rod 611 is moved up and moved to the middle of one side of the reaction tube 43 to attract magnetism.
S211中，对反应管43中的液体进行废除。In S211, the liquid in the reaction tube 43 is discarded.
S212中，磁吸组件6动作，使磁棒611向下运动至远离反应管43，反应管加热块312对应的加热膜315通电加热，使反应管43升温以将残留液体蒸发干净。In S212, the magnetic attraction assembly 6 is actuated to move the magnetic rod 611 downward to away from the reaction tube 43, the heating film 315 corresponding to the reaction tube heating block 312 is energized and heated, and the reaction tube 43 is heated to evaporate the residual liquid.
S213中，第二移液机构8动作，将吸头嘴834上的吸头废弃至吸头腔414中。In S213 , the second pipetting mechanism 8 operates to discard the tips on the tip nozzle 834 into the tip chamber 414 .
S214中，第二竖直位移单元82向下运动，吸头嘴834插取新的吸头。In S214, the second vertical displacement unit 82 moves downward, and the suction nozzle 834 inserts a new suction head.
S215中，第二移液机构8动作，吸头吸取洗脱液到反应管43中，并反复吹打反应管43中的液体至少1次。In S215, the second pipetting mechanism 8 is actuated, the suction head sucks the eluent into the reaction tube 43, and repeatedly blows the liquid in the reaction tube 43 at least once.
S216中，反应管加热块312对应的加热膜315通电加热，使反应管43加热，以洗脱核酸。In S216, the heating membrane 315 corresponding to the heating block 312 of the reaction tube is heated by electricity, so that the reaction tube 43 is heated to elute the nucleic acid.
S217中，磁吸组件6动作，将磁棒611移动至反应管43的一侧，进行吸磁，且将全部磁珠汇集到反应管43的管壁上部，使磁珠远离管底。In S217, the magnetic attraction assembly 6 is actuated, and the magnetic rod 611 is moved to one side of the reaction tube 43 to attract magnetism, and all the magnetic beads are collected on the upper part of the tube wall of the reaction tube 43 to keep the magnetic beads away from the bottom of the tube.
核酸扩增基于PCR技术或者恒温扩增技术等实现核酸分子的复制过程，包括：Nucleic acid amplification realizes the replication process of nucleic acid molecules based on PCR technology or constant temperature amplification technology, including:
S301中，盖板组件5向上动作，使核酸扩增管的开口开放。In S301, the cover plate assembly 5 moves upward to open the opening of the nucleic acid amplification tube.
S302中，第二移液机构8动作，吸头从反应管43中取定量核酸到核酸扩增管中，反复吹打。废弃吸头嘴834上的吸头。In S302, the second pipetting mechanism 8 is actuated, and the pipette tip takes the quantitative nucleic acid from the reaction tube 43 into the nucleic acid amplification tube, and pipetting is repeated. Discard the tips on the tip nozzle 834.
S303中，盖板组件5向下运动，将核酸扩增管的管口封闭。In S303, the cover plate assembly 5 moves downward to seal the mouth of the nucleic acid amplification tube.
S304中，系统启动核酸扩增程序，进行核酸扩增反应，期间，通过热处理模块控制核酸扩增反应所需的温度条件。In S304, the system starts the nucleic acid amplification program to perform the nucleic acid amplification reaction, during which the temperature conditions required for the nucleic acid amplification reaction are controlled by the heat treatment module.
杂交反应基于碱基互补原则实现分子的杂交识别，包括：The hybridization reaction realizes the hybridization recognition of molecules based on the principle of base complementarity, including:
S401中，在核酸扩增程序结束之前，开始吸杂交液A到杂交槽中进行预热，预热温度为35℃-95℃；In S401, before the nucleic acid amplification program ends, start to suck the hybridization solution A into the hybridization tank for preheating, and the preheating temperature is 35°C-95°C;
S402中，在核酸扩增反应结束后，盖板组件5动作，开放核酸扩增管42开口。In S402, after the nucleic acid amplification reaction is completed, the cover plate assembly 5 operates to open the opening of the nucleic acid amplification tube 42.
S403中，第二移液机构8动作，插取新的吸头，第二移液机构8和注射泵10动作，通过吸头将核酸扩增管中的扩增溶液吸入到含有A液的杂交槽412中，开始杂交。In S403, the second pipetting mechanism 8 is activated to insert a new pipette tip, the second pipetting mechanism 8 and the syringe pump 10 are activated, and the amplification solution in the nucleic acid amplification tube is sucked into the hybridization solution containing the A solution through the pipette head In tank 412, hybridization begins.
S404中，第二移液机构8动作，废弃吸头嘴834上的吸头。In S404, the second pipetting mechanism 8 is activated, and the tips on the tip nozzles 834 are discarded.
S405中，振荡加热组件3动作，带动杂交槽412沿X方向往复振荡。In S405, the oscillating heating element 3 is actuated to drive the hybridization tank 412 to oscillate back and forth along the X direction.
S406中，杂交反应第一预设时间后，第二移液机构8和液泵组9动作，废液管吸取杂交槽412中的液体至废液收集槽16。In S406 , after the hybridization reaction for the first preset time, the second pipetting mechanism 8 and the liquid pump group 9 act, and the waste liquid pipe sucks the liquid in the hybridization tank 412 to the waste liquid collection tank 16 .
S407中，第一移液机构7、第二移液机构8及液泵组9动作，通过第一试剂针711和第二试剂针8131将盛放有B杂交液的试剂瓶40中的液体吸取至杂交槽412。In S407, the first pipetting mechanism 7, the second pipetting mechanism 8 and the liquid pump group 9 are activated, and the liquid in the reagent bottle 40 containing the B hybridization liquid is sucked through the first reagent needle 711 and the second reagent needle 8131 to hybridization tank 412.
S408中，重复执行S405-S407，直至将试剂瓶存放区中分别承载有C杂交液、D杂交液和E杂交液的试剂瓶40中的液体依次抽取至杂交槽412中，在此过程中，振荡加热组件3持续执行X向振荡动作。In S408, S405-S407 are repeatedly executed until the liquids in the reagent bottles 40 carrying the C hybridization solution, the D hybridization solution and the E hybridization solution in the reagent bottle storage area are sequentially extracted into the hybridization tank 412. During this process, The oscillating heating assembly 3 continues to perform the X-direction oscillating action.
S409中，杂交反应结束，振荡加热组件3停止动作。In S409, the hybridization reaction ends, and the shaking heating element 3 stops operating.
S410中，第一移液机构7动作，通过第一试剂针711抽取装有纯化水的试剂瓶40中的纯化水，采用纯化水对试剂管42路组件中的管路进行清洗。In S410, the first pipetting mechanism 7 operates, and the purified water in the reagent bottle 40 containing the purified water is extracted through the first reagent needle 711, and the pipelines in the reagent tube 42-way assembly are cleaned with the purified water.
S411中，通过显示屏60，点击执行消毒操作，对检测主体进行消毒。In S411, through the display screen 60, click to perform a disinfection operation to disinfect the detection subject.

Claims (17)

  1. 一种核酸检测仪，包括：A nucleic acid detector, comprising:
    安装架(2)；mounting bracket (2);
    试剂板(4)；reagent plate (4);
    设置于所述试剂板(4)上的核酸扩增管、杂交槽(412)、反应管(43)及试剂管(42)；a nucleic acid amplification tube, a hybridization tank (412), a reaction tube (43) and a reagent tube (42) arranged on the reagent plate (4);
    盖板组件(5)，设置在所述安装架(2)上，且能够沿朝向或远离所述核酸扩增管的方向运动，以封闭或开放所述核酸扩增管的管口；a cover plate assembly (5), arranged on the mounting frame (2), and capable of moving in a direction toward or away from the nucleic acid amplification tube, so as to close or open the nozzle of the nucleic acid amplification tube;
    热处理单元(31)，设置在所述安装架(2)上，且设置为对所述试剂板(4)上液体进行加热或冷却处理；a heat treatment unit (31), arranged on the mounting frame (2), and configured to heat or cool the liquid on the reagent plate (4);
    移液装置，设置在所述安装架(2)上，且设置为对所述试剂板(4)上的液体进行吸取及转移；a pipetting device, arranged on the mounting frame (2), and configured to suck and transfer the liquid on the reagent plate (4);
    振荡单元(32)，设置在所述安装架(2)上且与试剂板(4)相对固定，所述振荡单元(32)设置为带动所述试剂板(4)沿水平方向往复振荡；an oscillating unit (32), arranged on the mounting frame (2) and relatively fixed to the reagent plate (4), the oscillating unit (32) being arranged to drive the reagent plate (4) to oscillate back and forth in a horizontal direction;
    磁吸组件(6)，设置在所述安装架(2)上，且能沿朝向或远离所述试剂板(4)的方向运动，所述磁吸组件(6)设置为对所述试剂板(4)上的反应液中的磁珠进行吸磁和放磁。A magnetic attraction assembly (6), arranged on the mounting frame (2) and capable of moving in a direction toward or away from the reagent plate (4), the magnetic attraction assembly (6) being arranged to face the reagent plate (4). (4) The magnetic beads in the reaction solution above perform magnetic attraction and magnetic release.
  2. 根据权利要求1所述的核酸检测仪，其中，所述试剂板(4)沿X方向延伸设置，所述试剂板(4)沿Y方向并排设置有多个，所述盖板组件(5)设置在多个所述试剂板(4)沿所述X方向的第一侧，所述磁吸组件(6)设置在多个所述试剂板(4)沿所述X方向的第二侧。The nucleic acid detector according to claim 1, wherein the reagent plates (4) are extended along the X direction, a plurality of the reagent plates (4) are arranged side by side along the Y direction, and the cover plate assembly (5) The magnetic attraction assembly (6) is arranged on the first side of the plurality of reagent plates (4) along the X direction, and the magnetic attraction assembly (6) is arranged on the second side of the plurality of reagent plates (4) along the X direction.
  3. 根据权利要求2所述的核酸检测仪，其中，所述盖板组件(5)包括：The nucleic acid detector according to claim 2, wherein the cover plate assembly (5) comprises:
    盖封组件(51)，所述盖封组件(51)包括位于所述核酸扩增管上方的盖封件(514)，所述盖封件(514)由弹性材料制成，所述盖封件(514)设置为密封所述核酸扩增管的管口；A capping assembly (51) comprising a capping member (514) located above the nucleic acid amplification tube, the capping member (514) being made of an elastic material, the capping member (514) being The member (514) is configured to seal the mouth of the nucleic acid amplification tube;
    盖板驱动组件(52)，与所述盖封组件(51)连接，并设置为驱动所述盖封组件(51)平移移动或转动，以使所述盖封件(514)密封或开启所述核酸扩增管的管口。A cover plate driving component (52) is connected to the cover sealing component (51) and is configured to drive the cover sealing component (51) to move or rotate in translation, so that the cover sealing member (514) can seal or open all The mouth of the nucleic acid amplification tube.
  4. 根据权利要求2所述的核酸检测仪，其中，所述核酸检测仪包括振荡加热组件(3)，所述振荡加热组件(3)包括所述热处理单元(31)、所述振荡单元(32)及位于连接于所述热处理单元(31)和所述振荡单元(32)之间的隔热单元(33)，所述试剂板(4)设置在所述热处理单元(31)上方并相对所述热 处理单元(31)固定。The nucleic acid detector according to claim 2, wherein the nucleic acid detector comprises an oscillating heating assembly (3), and the oscillating heating assembly (3) comprises the heat treatment unit (31), the oscillation unit (32) and a heat insulation unit (33) connected between the heat treatment unit (31) and the oscillation unit (32), the reagent plate (4) is arranged above the heat treatment unit (31) and opposite to the heat treatment unit (31) The heat treatment unit (31) is fixed.
  5. 根据权利要求4所述的核酸检测仪，其中，所述热处理单元(31)包括沿X方向并排且间隔设置的多个加热单元，所述多个加热单元分别对应设置在所述核酸扩增管、所述杂交槽(412)、所述反应管(43)及所述试剂管(42)的下方，且每个所述加热单元沿Y方向延伸。The nucleic acid detector according to claim 4, wherein the heat treatment unit (31) comprises a plurality of heating units arranged side by side and at intervals along the X direction, and the plurality of heating units are respectively arranged in the nucleic acid amplification tube correspondingly , below the hybridization tank (412), the reaction tube (43) and the reagent tube (42), and each of the heating units extends along the Y direction.
  6. 根据权利要求5所述的核酸检测仪，其中，所述加热单元包括位于所述核酸扩增管下方的核酸扩增管加热块(311)，所述热处理单元(31)还包括制冷单元(317)和散热单元(316)，所述散热单元(316)位于所述核酸扩增管加热块(311)的一侧且沿所述Y方向延伸，所述制冷单元(317)位于所述散热单元(316)和所述核酸扩增管加热块(311)之间。The nucleic acid detector according to claim 5, wherein the heating unit comprises a nucleic acid amplification tube heating block (311) located below the nucleic acid amplification tube, and the heat treatment unit (31) further comprises a refrigeration unit (317) ) and a heat dissipation unit (316), the heat dissipation unit (316) is located on one side of the nucleic acid amplification tube heating block (311) and extends along the Y direction, and the refrigeration unit (317) is located in the heat dissipation unit (316) and the nucleic acid amplification tube heating block (311).
  7. 根据权利要求5所述的核酸检测仪，其中，所述隔热单元(33)包括多个沿X方向间隔设置的隔热子单元，所述隔热子单元与所述加热单元一一对应，且所述加热单元可拆卸连接于所述隔热子单元的上方，每个所述隔热子单元由隔热材料制成。The nucleic acid detector according to claim 5, wherein the heat insulating unit (33) comprises a plurality of heat insulating sub-units arranged at intervals along the X direction, and the heat insulating sub-units are in one-to-one correspondence with the heating units, And the heating unit is detachably connected above the heat insulating sub-units, and each of the heat insulating sub-units is made of heat insulating material.
  8. 根据权利要求2所述的核酸检测仪，其中，所述磁吸组件(6)包括：The nucleic acid detector according to claim 2, wherein the magnetic attraction component (6) comprises:
    磁棒组件(61)，所述磁棒组件(61)包括多个竖直设置的磁棒(611)，所述多个磁棒(611)沿Y方向并排设置；及a magnet bar assembly (61), the magnet bar assembly (61) comprising a plurality of vertically arranged magnet bars (611), the plurality of magnet bars (611) being arranged side by side along the Y direction; and
    磁棒驱动组件(62)，与所述磁棒组件(61)连接，并设置为驱动所述磁棒组件(61)沿竖直方向运动。A magnet rod driving assembly (62) is connected with the magnet rod assembly (61) and is configured to drive the magnet rod assembly (61) to move in a vertical direction.
  9. 根据权利要求8所述的核酸检测仪，其中，所述磁棒组件(61)还包括沿Y方向设置的安装条(612)，所述安装条(612)上开设有安装孔(6121)，所述磁棒(611)的下端过盈插设于所述安装孔(6121)中；The nucleic acid detector according to claim 8, wherein the magnetic rod assembly (61) further comprises a mounting bar (612) arranged along the Y direction, and a mounting hole (6121) is opened on the mounting bar (612), The lower end of the magnet bar (611) is inserted into the mounting hole (6121) with interference;
    所述安装条(612)上沿竖直方向贯通开设有穿口(6122)，所述穿口(6122)的第一端贯通所述安装条(612)的侧壁，所述穿口(6122)的第二端与所述安装孔(6121)连通，所述穿口(6122)的宽度小于所述安装孔(6121)的孔径，且所述穿口(6122)与所述安装孔(6121)一一对应设置。A through hole (6122) is formed through the mounting bar (612) in the vertical direction, the first end of the through hole (6122) penetrates through the side wall of the mounting bar (612), and the through hole (6122) ) communicates with the mounting hole (6121), the width of the through hole (6122) is smaller than the diameter of the mounting hole (6121), and the through hole (6122) is connected to the mounting hole (6121). ) correspond to the settings one by one.
  10. 根据权利要求2所述的核酸检测仪，其中，所述核酸检测仪内设置有试剂瓶存放区，所述试剂瓶存放区中存放有承载试剂的试剂瓶(40)，所述移液装置包括：The nucleic acid detector according to claim 2, wherein a reagent bottle storage area is provided in the nucleic acid detector, and a reagent bottle (40) carrying a reagent is stored in the reagent bottle storage area, and the pipetting device comprises: :
    第一移液机构(7)，可相对所述安装架(2)水平移动及竖直升降，并设置为抽取所述试剂瓶(40)中的液体；及a first pipetting mechanism (7), which can move horizontally and vertically relative to the mounting frame (2), and is configured to extract the liquid in the reagent bottle (40); and
    第二移液机构(8)，可相对所述安装架(2)水平移动及竖直升降，设置为吸取和转移所述试剂板(4)上的液体。The second pipetting mechanism (8) can move horizontally and vertically relative to the mounting frame (2), and is configured to suck and transfer the liquid on the reagent plate (4).
  11. 根据权利要求10所述的核酸检测仪，其中，所述第二移液机构(8)包括吸头取放单元(83)，所述吸头取放单元(83)包括吸头座(831)、连接于所述吸头座(831)上的吸头套杆(832)，插设于所述吸头套杆(832)下端的吸头嘴(834)穿设于所述吸头套杆(832)内且下端与所述吸头嘴(834)密封插接的吸头针(833)，所述第二移液机构(8)设置为带动所述吸头取放单元(83)水平及竖直运动；The nucleic acid detector according to claim 10, wherein the second pipetting mechanism (8) comprises a tip pick-and-place unit (83), and the tip pick-and-place unit (83) comprises a tip holder (831) , a suction head cover rod (832) connected to the suction head seat (831), and a suction head nozzle (834) inserted into the lower end of the suction head cover rod (832) and penetrated through the suction head cover rod (832) A tip needle (833) whose inner and lower ends are sealed and plugged with the tip nozzle (834), and the second pipetting mechanism (8) is configured to drive the tip pick-and-place unit (83) horizontally and vertically sports;
    所述移液装置还包括注射泵(10)，所述核酸检测仪还包括吹吸管(701)，所述吸头针(833)的上端通过所述吹吸管(701)与所述注射泵(10)连接。The pipetting device further includes a syringe pump (10), the nucleic acid detector further includes a suction pipe (701), and the upper end of the suction needle (833) is connected to the syringe pump (701) through the suction pipe (701). 10) Connect.
  12. 根据权利要求11所述的核酸检测仪，其中，所述第一移液机构(7)包括至少两个第一试剂针(711)，所述第一移液机构(7)设置为带动所述第一试剂针(711)水平及竖直运动；The nucleic acid detector according to claim 11, wherein the first pipetting mechanism (7) comprises at least two first reagent needles (711), and the first pipetting mechanism (7) is configured to drive the The first reagent needle (711) moves horizontally and vertically;
    所述第二移液机构(8)包括至少两个试剂针束(813)，每个所述试剂针束(813)包括至少两个第二试剂针(8131)；The second pipetting mechanism (8) includes at least two reagent needle bundles (813), and each of the reagent needle bundles (813) includes at least two second reagent needles (8131);
    所述移液装置还包括液泵组(9)，所述液泵组(9)包括多个试剂进液口和多个与所述试剂进液口对应的试剂出液口，所述核酸检测仪还包括进液管(702)、主出液管(703)和多个分出液管(704)，每个所述第一试剂针(711)通过所述进液管(702)与一所述试剂进液口连通，每个所述试剂出液口连通有一所述主出液管(703)的一端，每一所述主出液管(703)的一端连通有多个分出液管(704)的第一端，所述分出液管(704)的第二端连通所述至少两个所述试剂针束(813)中的其中一个的所述第二试剂针(8131)，每个所述主出液管(703)连通的所述分出液管(704)的个数和所述试剂针束(813)的个数相同。The pipetting device further includes a liquid pump group (9), the liquid pump group (9) includes a plurality of reagent liquid inlets and a plurality of reagent liquid outlet ports corresponding to the reagent liquid inlets, and the nucleic acid detection The instrument further includes a liquid inlet pipe (702), a main liquid outlet pipe (703) and a plurality of branch liquid pipes (704), and each of the first reagent needles (711) passes through the liquid inlet pipe (702) and a liquid outlet pipe (702). The reagent liquid inlets are connected to each other, each of the reagent liquid outlets is connected to one end of the main liquid outlet pipe (703), and one end of each of the main liquid outlet pipes (703) is connected to a plurality of branch liquids The first end of the pipe (704), and the second end of the aliquot pipe (704) communicates with the second reagent needle (8131) of one of the at least two reagent needle bundles (813) , the number of the branch liquid pipes (704) connected to each of the main liquid discharge pipes (703) is the same as the number of the reagent needle bundles (813).
  13. 根据权利要求12所述的核酸检测仪，其中，所述第二试剂针(8131)包括竖直设置的主体部(81311)和连接于所述主体部(81311)下端的导向部(81312)，所述主体部(81311)与所述导向部(81312)一体成型，所述导向部(81312)的上端与所述主体部(81311)连接，所述导向部(81312)的下端倾斜向下延伸，且位于同一试剂针束(813)中的多个第二试剂针(8131)的所述导向部(81312)的延伸方向不同。The nucleic acid detector according to claim 12, wherein the second reagent needle (8131) comprises a vertically arranged main body part (81311) and a guide part (81312) connected to the lower end of the main body part (81311), The main body part (81311) and the guide part (81312) are integrally formed, the upper end of the guide part (81312) is connected with the main body part (81311), and the lower end of the guide part (81312) extends downwards obliquely , and the extending directions of the guide portions (81312) of the plurality of second reagent needles (8131) located in the same reagent needle bundle (813) are different.
  14. 根据权利要求12所述的核酸检测仪，其中，所述第二移液机构(8)还包括废液针(812)，所述废液针(812)竖直设置，所述第二移液机构(8)设 置为带动所述废液针(812)沿水平方向和竖直方向运动，所述液泵组(9)包括废液进口和废液出口，所述核酸检测仪还包括废液进管、废液出管(707)及废液瓶(80)，所述废液进口和所述废液针(812)通过所述废液进管连通，所述废液出口通过所述废液出管(707)连通所述废液瓶(80)。The nucleic acid detector according to claim 12, wherein the second pipetting mechanism (8) further comprises a waste liquid needle (812), the waste liquid needle (812) is arranged vertically, and the second pipetting mechanism (8) further comprises a waste liquid needle (812) The mechanism (8) is configured to drive the waste liquid needle (812) to move in a horizontal direction and a vertical direction, the liquid pump group (9) includes a waste liquid inlet and a waste liquid outlet, and the nucleic acid detector further includes a waste liquid The inlet pipe, the waste liquid outlet pipe (707) and the waste liquid bottle (80), the waste liquid inlet and the waste liquid needle (812) are communicated through the waste liquid inlet pipe, and the waste liquid outlet is connected through the waste liquid inlet pipe. The liquid outlet pipe (707) communicates with the waste liquid bottle (80).
  15. 根据权利要求1-14任一项所述的核酸检测仪，还包括外壳(1)，所述安装架(2)、所述试剂板(4)、所述盖板组件(5)、所述热处理单元(31)、所述移液装置、所述振荡单元(32)及所述磁吸组件(6)设置在所述外壳(1)内部，所述外壳(1)上设置有开口，所述开口处设置有设置为封闭或开启所述开口的门体，所述试剂板(4)能通过所述开口进出所述外壳(1)；The nucleic acid detector according to any one of claims 1-14, further comprising a housing (1), the mounting bracket (2), the reagent plate (4), the cover plate assembly (5), the The heat treatment unit (31), the pipetting device, the oscillation unit (32) and the magnetic attraction assembly (6) are arranged inside the casing (1), and the casing (1) is provided with an opening, so The opening is provided with a door body configured to close or open the opening, and the reagent plate (4) can enter and exit the housing (1) through the opening;
    所述外壳(1)内部设置有抽风过滤系统和杀菌消毒系统中的至少一种。At least one of a ventilation filtration system and a sterilization and disinfection system is provided inside the casing (1).
  16. 根据权利要求2-14任一项所述的核酸检测仪，还包括耗材面板(50)，所述耗材面板(50)上沿Y方向并排设置有多个试剂板口(501)，所述试剂板口(501)沿X方向延伸，所述试剂板(4)包括承载主板(41)，所述核酸扩增管、所述杂交槽(412)、所述反应管(43)及所述试剂管(42)设置在所述承载主板(41)上，所述承载主板(41)的下表面搭设于所述耗材面板(50)上，且所述核酸扩增管、所述杂交槽(412)、所述反应管(43)及所述试剂管(42)伸入所述试剂板口(501)中。The nucleic acid detector according to any one of claims 2-14, further comprising a consumables panel (50), and a plurality of reagent plate ports (501) are arranged side by side along the Y direction on the consumables panel (50). The plate mouth (501) extends along the X direction, and the reagent plate (4) includes a carrying main plate (41), the nucleic acid amplification tube, the hybridization tank (412), the reaction tube (43) and the reagent The tube (42) is arranged on the carrying main board (41), the lower surface of the carrying main board (41) is placed on the consumables panel (50), and the nucleic acid amplification tube, the hybridization tank (412) ), the reaction tube (43) and the reagent tube (42) extend into the reagent plate port (501).
  17. 一种核酸检测方法，其中，应用于如权利要求1-16任一项所述的核酸检测仪，包括：A nucleic acid detection method, wherein, applied to the nucleic acid detector according to any one of claims 1-16, comprising:
    检测前处理，所述检测前处理包括：打开所述核酸扩增管的管盖；及所述盖板组件(5)将所述核酸扩增管的管口封闭；Pre-detection treatment, the pre-detection treatment includes: opening the cap of the nucleic acid amplification tube; and sealing the mouth of the nucleic acid amplification tube by the cover plate assembly (5);
    核酸提取和纯化，其中，基于磁珠法进行所述核酸提取和纯化，且所述核酸提取和纯化包括：所述移液装置将样本加入含有蛋白酶K和磁珠的所述试剂管(42)中，混匀；所述移液装置吸取混匀后的蛋白酶K、磁珠和样本液体的混合液至含有磁珠结合液的反应管(43)中；热处理单元(31)调控所述反应管(43)反应所需温度条件，以使细胞裂解，释放核酸；及在释放核酸后，通过所述磁吸组件(6)沿靠近或远离所述试剂板(4)的往复运动并配合所述移液装置的液体抽取和转移实现核酸在磁珠上吸附、洗涤和洗脱，以得到纯化的核酸；Nucleic acid extraction and purification, wherein the nucleic acid extraction and purification is performed based on a magnetic bead method, and the nucleic acid extraction and purification comprises: the pipetting device adds a sample to the reagent tube (42) containing proteinase K and magnetic beads the pipetting device sucks the mixed solution of proteinase K, magnetic beads and sample liquid into the reaction tube (43) containing the magnetic bead binding solution; the heat treatment unit (31) regulates the reaction tube (43) the temperature conditions required for the reaction to lyse the cells and release the nucleic acid; and after the nucleic acid is released, the magnetic attraction component (6) is used for reciprocating motion close to or away from the reagent plate (4) and cooperates with the The liquid extraction and transfer of the pipetting device realizes the adsorption, washing and elution of nucleic acid on the magnetic beads to obtain purified nucleic acid;
    核酸扩增，所述核酸扩增包括：盖板组件(5)运动，使核酸扩增管的管口开放；移液装置吸取一定量的核酸至核酸扩增管中；盖板组件(5)运动，将所述核酸扩增管的管口封闭；及启动核酸扩增程序，进行核酸扩增反应，期间， 通过所述热处理单元(31)控制核酸扩增反应所需的温度条件；及Nucleic acid amplification, the nucleic acid amplification comprises: the cover plate assembly (5) moves to open the mouth of the nucleic acid amplification tube; the pipetting device sucks a certain amount of nucleic acid into the nucleic acid amplification tube; the cover plate assembly (5) moving, closing the mouth of the nucleic acid amplification tube; and starting the nucleic acid amplification procedure to carry out the nucleic acid amplification reaction, during which the temperature conditions required for the nucleic acid amplification reaction are controlled by the heat treatment unit (31); and
    杂交反应，所述杂交反应包括：盖板组件(5)运动，将所述核酸扩增管的管口开放；所述移液装置抽取核酸扩增管的中的扩增溶液至杂交槽(412)中；及移液装置依次抽取不同的杂交液至杂交槽(412)中，热处理模块(31)调控反应所需温度，所述振荡单元(32)带动所述试剂板(4)水平往复振荡，以实现扩增产物和杂交膜上探针的特异性结合、洗涤和显色。A hybridization reaction, the hybridization reaction includes: the cover plate assembly (5) moves to open the mouth of the nucleic acid amplification tube; the pipetting device extracts the amplification solution in the nucleic acid amplification tube to the hybridization tank (412 ); and the pipetting device extracts different hybridization liquids successively into the hybridization tank (412), the heat treatment module (31) regulates the temperature required for the reaction, and the oscillation unit (32) drives the reagent plate (4) to vibrate horizontally back and forth , in order to achieve specific binding, washing and color development of amplification products and probes on the hybridization membrane.
PCT/CN2020/140787 2020-10-27 2020-12-29 Nucleic acid detector and nucleic acid detection method WO2022088477A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202011165266.3A CN112300911A (en) 2020-10-27 2020-10-27 Nucleic acid detector and nucleic acid detection method
CN202011165266.3 2020-10-27

Publications (1)

Publication Number Publication Date
WO2022088477A1 true WO2022088477A1 (en) 2022-05-05

Family

ID=74331112

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2020/140787 WO2022088477A1 (en) 2020-10-27 2020-12-29 Nucleic acid detector and nucleic acid detection method

Country Status (2)

Country Link
CN (1) CN112300911A (en)
WO (1) WO2022088477A1 (en)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109825431A (en) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 Oligonucleotides assemble in situ instrument
CN113376186A (en) * 2021-05-12 2021-09-10 赵静 High-precision intelligent erythrocyte folic acid detector and detection method thereof
CN114933954A (en) * 2022-07-25 2022-08-23 珠海宝锐生物科技有限公司 Nucleic acid extraction device
CN115161188A (en) * 2022-09-07 2022-10-11 至美时代生物智能科技(北京)有限公司 Air microorganism detection system and method
CN115197833A (en) * 2022-08-22 2022-10-18 杭州柏炬科技有限公司 Automatic warehouse building instrument and method for step sampling
CN115386457A (en) * 2022-09-23 2022-11-25 广州湾区生物科技有限公司 Nucleic acid extraction instrument
CN115388594A (en) * 2022-08-17 2022-11-25 浙江省人民医院 Sample temperature monitoring device
CN115508295A (en) * 2022-10-28 2022-12-23 武汉市农业科学院 Rapid detection instrument for activity of feed enzyme preparation
CN115651835A (en) * 2022-11-10 2023-01-31 杭州奥盛仪器有限公司 Gene detection processing device and method
CN116008526A (en) * 2023-03-24 2023-04-25 苏州浦隆生物有限公司 Microfluidic biomolecular chemiluminescence multi-joint detection device and multi-joint detection method
CN116273211A (en) * 2023-05-19 2023-06-23 潍坊安普未来生物科技有限公司 Detection device and use method
CN116773839A (en) * 2023-08-22 2023-09-19 世纪亿康（天津）医疗科技发展有限公司 Integrated automatic analyzer for blood detection and analysis method
CN116948816A (en) * 2023-06-06 2023-10-27 仲恺农业工程学院 Superoxide dismutase tolerance detection device and method
CN117535134A (en) * 2024-01-09 2024-02-09 哈尔滨因极科技有限公司 Cardiovascular disease detection device

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102533022B1 (en) * 2021-05-27 2023-05-16 옴니시스템 주식회사 Apparatus for controlling temperature of pcr device using thermoelectric element
CN113433119A (en) * 2021-06-25 2021-09-24 广州和实生物技术有限公司 Nucleic acid qualitative detection device and method for realizing visual display of result and application
CN113607724A (en) * 2021-06-29 2021-11-05 中国人民解放军东部战区疾病预防控制中心 Integrated detection device and detection method for LAMP or RT-LAMP
CN117795050A (en) * 2021-07-15 2024-03-29 南京金斯瑞生物科技有限公司 Sample processing device and application method thereof
CN117757597A (en) * 2021-07-15 2024-03-26 南京金斯瑞生物科技有限公司 Automatic extraction equipment
CN113684120A (en) * 2021-08-20 2021-11-23 圣湘生物科技股份有限公司 Nucleic acid detection apparatus and nucleic acid detection method
CN114308172A (en) * 2022-03-03 2022-04-12 迈德(山东)科技股份有限公司 Full-automatic reagent subpackaging device and subpackaging method
CN114570449B (en) * 2022-04-26 2022-08-16 广州国家实验室 Liquid transfer device and multi-path parallel liquid transfer device
CN115814727B (en) * 2023-02-23 2023-05-16 苏州硒瑞恩生物科技有限公司 Nucleic acid synthesizer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208716804U (en) * 2018-04-28 2019-04-09 绍兴迅敏康生物科技有限公司 Automatic capping device
CN111032209A (en) * 2017-07-10 2020-04-17 简·探针公司 Analysis system and method
CN111699242A (en) * 2017-10-11 2020-09-22 上海仁度生物科技有限公司 Automated nucleic acid sample preparation, detection and analysis system

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007002384A2 (en) * 2005-06-24 2007-01-04 Transnetyx, Inc. Methods for genotype screening of a strain disposed on an adsorbent carrier
DE102006034245C5 (en) * 2006-07-21 2014-05-28 Stratec Biomedical Systems Ag Positioning device for positioning pipettes
CN102906573B (en) * 2010-02-23 2015-02-18 瑞昂尼克公司 Self-contained biological assay apparatus, methods, and applications
EP2749887A3 (en) * 2010-07-23 2014-10-01 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
CN102134552B (en) * 2010-12-31 2014-05-21 福建泰普生物科学有限公司 Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same
TWI628283B (en) * 2012-07-17 2018-07-01 環球生物研究股份有限公司 Optical measuring device for reaction vessel and method therefor
CN103013814B (en) * 2012-12-20 2014-07-02 嘉兴凯实生物科技有限公司 Nucleic acid purification device
CN103551216A (en) * 2013-11-06 2014-02-05 北京自由度科学机器有限公司 Full automatic liquid relief instrument and application thereof
CN206069843U (en) * 2016-01-20 2017-04-05 广州市基准医疗有限责任公司 Nucleic acid sequencing equipment
CN205749540U (en) * 2016-05-11 2016-11-30 中储粮成都粮食储藏科学研究所 A kind of shifting liquid liquid-adding device automatically
JP7022880B2 (en) * 2016-11-18 2022-02-21 モラレー リサーチ インク． Biological sample preparation system and related methods
CN106916743B (en) * 2017-03-19 2019-02-12 北京化工大学 Integrated nucleic acid extraction and augmentation detection system
CN108865659A (en) * 2018-09-19 2018-11-23 基蛋生物科技股份有限公司 Nucleic acid extraction and amplification fluorescent detection system
CN209890609U (en) * 2019-03-14 2020-01-03 广州蓝勃生物科技有限公司 Cell extraction injection mobile device
CN111004797B (en) * 2019-12-06 2021-07-30 深圳市宝安区沙井人民医院 Nucleic acid extraction device and nucleic acid extraction method
CN112175809A (en) * 2020-10-27 2021-01-05 广州和实生物技术有限公司 Integrated nucleic acid detection workstation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111032209A (en) * 2017-07-10 2020-04-17 简·探针公司 Analysis system and method
CN111699242A (en) * 2017-10-11 2020-09-22 上海仁度生物科技有限公司 Automated nucleic acid sample preparation, detection and analysis system
CN208716804U (en) * 2018-04-28 2019-04-09 绍兴迅敏康生物科技有限公司 Automatic capping device

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109825431A (en) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 Oligonucleotides assemble in situ instrument
CN113376186A (en) * 2021-05-12 2021-09-10 赵静 High-precision intelligent erythrocyte folic acid detector and detection method thereof
CN113376186B (en) * 2021-05-12 2022-12-23 无锡科金生物科技有限公司 High-precision intelligent erythrocyte folic acid detector and detection method thereof
CN114933954A (en) * 2022-07-25 2022-08-23 珠海宝锐生物科技有限公司 Nucleic acid extraction device
CN114933954B (en) * 2022-07-25 2022-11-11 珠海宝锐生物科技有限公司 Nucleic acid extraction device
CN115388594B (en) * 2022-08-17 2023-09-01 浙江省人民医院 Sample temperature monitoring device
CN115388594A (en) * 2022-08-17 2022-11-25 浙江省人民医院 Sample temperature monitoring device
CN115197833A (en) * 2022-08-22 2022-10-18 杭州柏炬科技有限公司 Automatic warehouse building instrument and method for step sampling
CN115161188A (en) * 2022-09-07 2022-10-11 至美时代生物智能科技(北京)有限公司 Air microorganism detection system and method
CN115386457A (en) * 2022-09-23 2022-11-25 广州湾区生物科技有限公司 Nucleic acid extraction instrument
CN115386457B (en) * 2022-09-23 2023-11-28 广州湾区生物科技有限公司 Nucleic acid extraction instrument
CN115508295A (en) * 2022-10-28 2022-12-23 武汉市农业科学院 Rapid detection instrument for activity of feed enzyme preparation
CN115651835A (en) * 2022-11-10 2023-01-31 杭州奥盛仪器有限公司 Gene detection processing device and method
CN115651835B (en) * 2022-11-10 2024-03-29 杭州奥盛仪器有限公司 Gene detection processing apparatus and method
CN116008526A (en) * 2023-03-24 2023-04-25 苏州浦隆生物有限公司 Microfluidic biomolecular chemiluminescence multi-joint detection device and multi-joint detection method
CN116273211B (en) * 2023-05-19 2023-07-21 潍坊安普未来生物科技有限公司 Detection device and use method
CN116273211A (en) * 2023-05-19 2023-06-23 潍坊安普未来生物科技有限公司 Detection device and use method
CN116948816A (en) * 2023-06-06 2023-10-27 仲恺农业工程学院 Superoxide dismutase tolerance detection device and method
CN116773839A (en) * 2023-08-22 2023-09-19 世纪亿康（天津）医疗科技发展有限公司 Integrated automatic analyzer for blood detection and analysis method
CN116773839B (en) * 2023-08-22 2023-11-03 世纪亿康（天津）医疗科技发展有限公司 Integrated automatic analyzer for blood detection and analysis method
CN117535134A (en) * 2024-01-09 2024-02-09 哈尔滨因极科技有限公司 Cardiovascular disease detection device
CN117535134B (en) * 2024-01-09 2024-04-02 哈尔滨因极科技有限公司 Cardiovascular disease detection device

Also Published As

Publication number Publication date
CN112300911A (en) 2021-02-02

Similar Documents

Publication Publication Date Title
WO2022088477A1 (en) Nucleic acid detector and nucleic acid detection method
WO2022088472A1 (en) Integrated nucleic acid detection workstation
WO2022237181A1 (en) Dna and rna nucleic acid co-extraction and detection system
US20170045542A1 (en) Modular Liquid Handling System
JP5908613B2 (en) Apparatus and method for automatic analysis of biological sample
JP4125735B2 (en) Modular equipment
JP5268870B2 (en) Nucleic acid automatic extraction system and method
JPH08320274A (en) Method and device for liquid treatment using pipette
CN102954954A (en) Magnetic separation-based multi-sample multi-site high-flux nucleic acid analysis system
WO2017024899A1 (en) Reagent workstation
JP5877192B2 (en) Reaction vessel and method for producing the same
CA3045249A1 (en) Biologic sample preparation system and related method
CN114134032B (en) Gene sequencing pretreatment device
WO2015072941A1 (en) Laboratory automation system
CN111065928A (en) Sample processing device integrating heater, oscillator and magnet
WO2004092710A1 (en) Dispensing cylinder, large capacity dispensing device, and method of using large capacity dispensing device
CN214060493U (en) Integrated nucleic acid detection workstation
CN114868021A (en) Automated library generator
CN214529016U (en) A vibration heating element and nucleic acid detector for reagent board
CN115651835A (en) Gene detection processing device and method
CN115178315A (en) Full-automatic high-flux workstation integration device
CN115572673A (en) Full-automatic sample library preparation system and application
CN117337395A (en) Dispensing device and sample processing and measuring system
CN220867423U (en) Full-automatic molecular diagnosis all-in-one equipment
CN218596417U (en) Full-automatic sample library preparation system

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20959634

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20959634

Country of ref document: EP

Kind code of ref document: A1