CN220867423U - Full-automatic molecular diagnosis all-in-one equipment - Google Patents

Abstract

The utility model belongs to the field of detection equipment, and discloses full-automatic molecular diagnosis all-in-one machine equipment which comprises a base frame, a sample cup separating module, a sample extracting module, a system constructing module and a detection module, wherein the base frame is provided with a sample processing space and a detection space, the sample cup separating module is positioned in the sample processing space and is used for inputting information into and separating a detection sample, the sample extracting module is positioned in the sample processing space and is used for extracting a sample to be detected after separating the cup, the system constructing module is positioned in the sample processing space, the system constructing module is used for processing an extract and sending the processed extract out, the detection module is positioned in the detection space and comprises a detection cabin, and the detection cabin is used for receiving the processed extract and performing sealing detection. The full-automatic molecular diagnosis all-in-one machine equipment can realize full-automatic detection, and is reasonable and reliable in structure.

Description

Full-automatic molecular diagnosis all-in-one equipment

Technical Field

The utility model relates to the field of detection equipment, in particular to full-automatic molecular diagnosis integrated machine equipment.

Background

The molecular diagnosis technology is a technical means capable of rapidly and accurately diagnosing pathogens, and comprises a plurality of links such as pathogen sample processing, nucleic acid extraction, PCR amplification, fluorescent marking and detection, result analysis and the like. When detecting pathogen samples, detection equipment is needed, and amplification detection is carried out on pathogen sample extracting solutions according to detection technology to obtain detection results. In the prior art, most molecular diagnostic instruments are semi-automatic instruments, and when molecular diagnosis is performed, operations such as transferring samples are needed to be manually participated, and the manual participations can increase testing errors, slow down detection speed and increase risks of biological pollution. In addition, the sealing performance of the existing amplification device is weaker, and the amplification product is easy to overflow, so that the mutual pollution inside the instrument is possibly caused, the detection accuracy and reliability are reduced, operators can be infected, and the detection safety is poor. Moreover, medical diagnosis and patient treatment generally depend on the detection of analyte concentrations or other parameters in a sample, whereas molecular diagnostic detection results are mainly derived from the detection device, since the life health of a patient etc. may depend on the accuracy and reliability of the results of such measurements, the accuracy and reliability of the detection is of paramount importance.

Disclosure of utility model

Aiming at least one defect or deficiency in the prior art, the utility model provides full-automatic molecular diagnosis all-in-one machine equipment which can realize full-automatic detection and has reasonable and reliable structure.

In order to achieve the above object, the present utility model provides a full-automatic molecular diagnosis all-in-one machine apparatus, comprising:

A base frame provided with a sample processing space and a detection space;

The sample cup separating module is positioned in the sample processing space and used for inputting information and separating cups for the detection sample;

The sample extraction module is positioned in the sample processing space and is used for extracting the sample to be detected after the cup separation;

The system construction module is positioned in the sample processing space and is used for processing the extract and sending out the processed extract; and

The detection module is positioned in the detection space and comprises a detection cabin, and the detection cabin is used for receiving the processed extract and performing sealing detection.

Alternatively, the detection module may be provided with a plurality of detection chambers arranged side by side and capable of independent detection, each detection chamber being provided with a plurality of detection bits.

Further, the sample cup separation module may include:

The sample tube code scanner is used for inputting information to the detection sample;

Clamping units which are arranged in pairs and are used for clamping a sample tube filled with a detection sample;

The first moving unit is provided with uncovering mechanical claws at the moving ends in pairs and used for driving the uncovering mechanical claws to transfer the sample tube to the clamping unit for uncovering; and

And the second movement unit is provided with a liquid transferring pump unit in pairs at the movement ends and is used for driving the liquid transferring pump unit to suck the detection sample positioned in the clamping unit into the sample extraction module.

In addition, the sample cup separating module can comprise a sample tube sample feeding carrier arranged in double rows;

and/or, the sample cup separation module may include a consumable unit for housing Tip head consumables and a Tip head waste trash can.

Alternatively, the architecture building module may include:

The detection reagent unit is used for accommodating the detection reagent tube;

the detection reagent uncapping unit is used for uncapping the detection reagent tube;

The reaction tube unit is used for accommodating the reaction tube, and the second movement unit is also used for driving the liquid transfer pump unit to inject the extract in the detection reagent tube into the reaction tube; and

And the reaction tube movement unit is used for transferring the reaction tube filled with the extract into the detection module.

Further, the full-automatic molecular diagnosis all-in-one machine equipment can further comprise a closed machine body shell, the base body frame is arranged in the machine body shell, the machine body shell comprises a shell metal plate, an observation window, a touch screen, a sample tube access cabin door, a consumable access cabin door and a reaction tube access cabin door, the observation window, the sample tube access cabin door and the consumable access cabin door are all arranged on the front end face of the shell metal plate aligned with the sample processing space, and the touch screen is arranged on the front end face of the shell metal plate aligned with the detection space.

Further, the full-automatic molecular diagnosis integrated machine device may further include a sample processing space laminar flow system disposed on the housing, the sample processing space laminar flow system being configured to form a negative pressure inside the housing to prevent an inside polluted air flow from flowing out without being filtered, the sample processing space laminar flow system including a sample processing space centrifugal fan and a sample processing space filter, the sample processing space filter being disposed at an air inlet of the sample processing space centrifugal fan.

In some embodiments, the base frame may include:

An upper supporting part, a sample cup separating module, a sample extracting module and a system constructing module are arranged on the table surface part, and a detecting module is arranged on the bracket part; and

And a lower supporting part positioned at the lower part of the base frame and used for supporting the upper supporting part.

Further, the full-automatic molecular diagnosis integrated machine device may further include a detection module housing located on the base frame and defining a detection space, the detection module housing being provided with a detection module side opening for receiving the reaction tube containing the extract, the detection module further including a third movement unit having a reaction tube gripper at a movement end, the third movement unit being configured to drive the reaction tube gripper to transfer the reaction tube containing the extract into the detection chamber.

Still further, the detection module may further comprise a detection module door mechanism provided on the detection module housing and adapted to open and close the detection module side opening.

Alternatively, the detection pod may comprise:

The cabin body is positioned at the bottom of the detection space and provided with a cabin body opening, and the cabin body opening is positioned at the top of the cabin body;

The detection unit is arranged in the cabin body and is used for detecting the extract in the reaction tube; and

And the hatch cover mechanism is arranged on the hatch body and used for opening and closing the hatch body opening.

In addition, the detection module can further comprise a negative pressure cabin which is arranged on one side of the cabin body and communicated with the interior of the cabin body, and a detection module centrifugal fan for pumping out the interior air of the cabin body is arranged in the negative pressure cabin.

Optionally, the bottom wall of the negative pressure chamber may be provided with a negative pressure chamber air outlet, the bottom wall of the detection module housing is provided with a first air pipe penetrating opening, the top wall of the detection module housing is provided with a second air pipe penetrating opening, the detection module further comprises an exhaust pipe, one end of which is communicated with the negative pressure chamber air outlet from the bottom wall of the negative pressure chamber, and the other end of the exhaust pipe is sequentially penetrated in the first air pipe penetrating opening and the second air pipe penetrating opening upward so as to exhaust the detection module housing.

Further, the detection module may further include a reaction tube waste barrel unit located in front, the reaction tube waste barrel unit includes a waste barrel mounting seat with a barrel cavity therein and a barrel body extendably disposed in the barrel cavity, the front side wall of the detection module housing is provided with a detection module front opening aligned with the barrel body extension opening of the waste barrel mounting seat, and the barrel body can extend from the detection module front opening or close the detection module front opening.

Still further, the top of the waste bucket mounting seat may be provided with a mounting seat top opening, and the reaction tube waste bucket unit further includes a mounting seat cover mechanism disposed on the waste bucket mounting seat and adapted to open and close the mounting seat top opening.

According to the technical scheme, the full-automatic molecular diagnosis integrated machine equipment comprises the base frame, and the sample cup separating module, the sample extracting module, the system constructing module and the detecting module which are arranged on the base frame, so that a plurality of links of pathogen sample processing, nucleic acid extraction, PCR amplification, fluorescent marking and detection can be automatically completed, manual operation intervention is not needed, the operation difficulty is low, full-process automatic diagnosis of sample inlet and sample outlet in nucleic acid detection can be realized, the personnel intervention degree is greatly reduced, the testing error and the biological pollution risk in the detection process are reduced, the safety and reliability are high, the diagnosis result can be obtained rapidly, the detection efficiency is effectively improved, the detection cost is reduced, and the defects of low automation degree and insufficient systematic diagnosis and treatment capability of the existing detecting means are effectively overcome. In addition, because the detection process of molecular diagnosis and the safety reliability and accuracy of the detection result are vital to the life and health of patients and medical staff, and the like, in order to avoid the potential risk of large detection result error under the space limitation condition caused by adopting an integrated design, the full-automatic molecular diagnosis integrated machine equipment designs a double-layer isolation protection structure, firstly, a sample processing space and a detection space are respectively and independently arranged on a base frame for first-layer isolation protection, and then a detection module in the detection space is provided with a detection cabin capable of carrying out sealing detection on an extract for second-layer isolation protection. The double-layer isolation protection structure is reasonable, the sealing performance is good, the protection performance is high, the technical difficulty problem of mutual pollution inside the full-automatic molecular diagnosis instrument is solved, the infection risk caused by overflow of amplified products in the detection process can be effectively reduced, the detection accuracy and the safety reliability are high, and the life health safety of medical staff and patients is effectively ensured.

Additional features and advantages of the utility model will be set forth in the detailed description which follows.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model, and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the description serve to explain, without limitation, the utility model. In the drawings:

FIG. 1 shows a schematic structural diagram of a fully automated molecular diagnostic all-in-one machine according to one embodiment of the present utility model;

FIG. 2 illustrates a front view of the fully automated molecular diagnostic all-in-one apparatus of FIG. 1;

FIG. 3 shows a schematic structural view of the base frame of FIG. 1;

FIG. 4 is a schematic diagram showing the internal structure of the fully automated molecular diagnostic all-in-one machine of FIG. 1;

Fig. 5 and 6 show enlarged schematic views of the internal structure of fig. 4, respectively;

FIG. 7 shows a schematic structural diagram of the detection module in FIG. 4;

FIG. 8 shows a schematic diagram of the structure of the detection module of FIG. 4 at another view angle;

FIG. 9 is a schematic diagram showing the internal structure of the detection module in FIG. 7;

FIG. 10 is a schematic view showing the internal structure of the detection module of FIG. 9 from another perspective;

FIG. 11 shows a schematic partial structure of the detection module housing of FIG. 9;

FIG. 12 shows a schematic structural view of the inspection chamber of FIG. 9;

FIG. 13 is a schematic view showing the construction of the reaction tube reject bin unit of FIG. 9; and

Fig. 14 shows a schematic structural view of the third moving unit in fig. 9.

Reference numerals illustrate:

100. full-automatic molecular diagnosis all-in-one equipment

21. Lower support portion of base frame 211

212. Stand portion 213 for stand portion

22. Sample cup module 222 sample tube code scanner

223. Mechanical gripper 224 liquid transferring pump unit

225. First motion unit 226 second motion unit

227. Clamping unit 228 consumable unit

229 Tip head waste garbage can 23 sample extraction module

24. System construction module 241 detects reagent unit

242. Detection reagent uncapping unit 243 reaction tube unit

244. Reaction tube movement unit 25 detection module

251. Detection cabin 2511 body

2512. Cabin cover mechanism of cabin opening 2513

2514. Cabin side opening 2531X shaft movement module

2532Y-axis motion module 2533Z-axis motion module

252. Third movement unit of reaction tube clamping jaw 253

254. Reaction tube waste barrel unit 2541 waste barrel mounting seat

2542. Sample injection carrier for barrel 221 sample tube

2543. Detection module cabin door mechanism of mounting seat cover mechanism 255

256. Detection module side opening of detection module housing 2561

2562. The first air pipe penetrating opening 2563 and the second air pipe penetrating opening

2564. Front opening of detection module

257. Negative pressure cabin 2571 negative pressure cabin air outlet

3. Sheet metal of machine body shell 31 shell

36. Viewing window for sample processing space laminar flow system 35

37. Touch screen 32 sample tube access cabin door

33. Consumable material inlet and outlet cabin door 34 reaction tube inlet and outlet cabin door

Detailed Description

The following describes the detailed implementation of the embodiments of the present utility model with reference to the drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the utility model, are not intended to limit the utility model.

It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.

In the embodiments of the present utility model, unless otherwise indicated, terms of orientation such as "upper, lower, top, bottom" or "upper, lower, top, bottom" are used generally with respect to the orientation shown in the drawings or with respect to the positional relationship of the various components with respect to one another in the vertical, vertical or gravitational directions.

As shown in fig. 1 to 4, the present utility model provides a novel full-automatic molecular diagnosis all-in-one apparatus, which includes a base frame 21, a sample cup separating module 22, a sample extracting module 23, a system constructing module 24, and a detecting module 25. The base frame 21 is provided with a sample processing space in which the sample cup separating module 22, the sample extracting module 23, and the system constructing module 24 are located, and a detection space in which the detection module 25 is located. The sample cup separating module 22 is used for inputting information into a detection sample and separating the detection sample, the sample extracting module 23 is used for extracting a sample to be detected after separating the cup, the system constructing module 24 is used for processing an extract and sending the processed extract, and the detection module 25 comprises a detection cabin 251 for receiving the processed extract and performing sealing detection. Therefore, the method can automatically complete a plurality of links of pathogen sample processing, nucleic acid extraction, PCR amplification, fluorescent marking and detection, realize sample inlet and sample outlet in nucleic acid detection, greatly reduce personnel intervention degree, reduce pollution risk in the detection process and improve detection efficiency. In addition, sample processing space and detection space set up on the base frame 21 independently respectively, and detection module 25 includes carries out sealed detection's to the extract detection cabin 251, adopts dual physical isolation mode, and is rational in infrastructure, and sealing performance is good, avoids amplifying the pollution problem that the product overflows and causes in the testing process, improves detection accuracy and reliability, guarantees operating personnel's life health safety.

The full-automatic molecular diagnosis integrated machine device can be used for detecting nucleic acid molecules or protein molecules, and the utility model is not limited to the detection. When the full-automatic molecular diagnosis integrated machine device is used for detecting nucleic acid molecules, the detection module can be a PCR amplification detection module.

Alternatively, the inspection module 25 may be provided with a plurality of inspection pods 251 arranged side by side and capable of independent inspection, each inspection pod 251 being provided with a plurality of inspection bits. As shown in fig. 9 to 12, the inspection module 25 may be provided with four inspection chambers 251 disposed side by side and capable of independent inspection, each inspection chamber 251 being provided with 4 inspection bits. Thus, 16 samples can be detected simultaneously, and the detection efficiency is high. And each detection cabin 251 is independently sealed and is independently detected, so that the problem of cross contamination caused by overflow of amplified products in the detection process is avoided, and the detection precision and reliability are ensured. In addition, in the detection process, when an emergency situation is met and special queue insertion detection is needed for an emergency sample, the whole line stop is not needed, one part of detection cabin 251 can be used for special detection purposes, the other part of detection cabin 251 does not need to stop, and the detection processing is continued, so that the detection flow is more reasonable, the sample injection detection can be carried out at any time, the flexibility is higher, and the detection efficiency is higher. It should be noted that, the detection module 25 may be provided with 2, 3, 5, 6 or more detection tanks 251, etc., and each detection tank 251 is provided with 2, 3, 5, 6 or more detection bits, etc., and the present utility model is not limited thereto.

Further, the fully automatic molecular diagnosis all-in-one apparatus further includes a closed body casing 3, and the base frame 21 is provided in the body casing 3. The machine body shell 3 is mainly isolated from the outside to prevent pollutants from overflowing, and the machine body shell 3 can be provided with an outside interaction intersection such as a cabin door, an observation window, a touch screen and the like. Specifically, as shown in fig. 2, the housing 3 includes a housing sheet metal 31, an observation window 35, a touch screen 37, a sample tube access cabin door 32, a consumable access cabin door 33, and a reaction tube access cabin door 34, the observation window 35, the sample tube access cabin door 32, and the consumable access cabin door 33 are all disposed on a front end surface of the housing sheet metal 31 aligned with the sample processing space, and the touch screen 37 is disposed on a front end surface of the housing sheet metal 31 aligned with the detection space.

Still further, the fully automatic molecular diagnosis all-in-one apparatus may further include a sample processing space laminar flow system 32 disposed on a ceiling of the body casing 3 aligned with the sample processing space, the sample processing space laminar flow system 32 being configured to form a negative pressure in the sample processing space inside the body casing 3 to prevent an unfiltered outflow of the internal contaminated air flow. The sample processing space laminar flow system comprises a high-power sample processing space centrifugal fan, a sample processing space filter (not shown in the figure) and the like, and the sample processing space filter is arranged at an air inlet of the sample processing space centrifugal fan, so that negative pressure can be formed in the sample processing space inside the machine body shell 3, air flow is ensured to enter the sample processing space inside the machine body shell 3 from the outside, and only flows out through the sample processing space filter, so that polluted air flow in the sample processing space is prevented from flowing out of the equipment without passing through other gap positions.

In addition, the fully-automatic molecular diagnosis integrated machine apparatus may further include a detection space laminar flow system provided on the top plate of the body casing 3 aligned with the detection space, the detection space laminar flow system being provided independently of the sample processing space laminar flow system 32. The detection space laminar flow system is used for forming negative pressure in the detection space inside the machine body shell 3 so as to prevent the inside polluted air flow from flowing out without being filtered. The detection space laminar flow system comprises a high-power detection space centrifugal fan, a detection space filter (not shown in the figure) and the like, and the detection space filter is arranged at an air inlet of the detection space centrifugal fan, so that negative pressure can be formed in the detection space inside the machine body shell 3, air flow is ensured to enter the detection space inside the machine body shell 3 from the outside, and only flows out through the detection space filter, so that polluted air flow in the detection space is prevented from flowing out of equipment without passing through other slit positions.

In addition, the full-automatic molecular diagnosis integrated machine apparatus may further include a first sensor for detecting the air pressure in the sample processing space, a second sensor for detecting the air pressure in the detection space, and a controller. The controller is respectively in communication connection with the first sensor, the second sensor, the sample processing space centrifugal fan and the detection space centrifugal fan. When the air pressure detected by the second sensor is greater than the air pressure detected by the first sensor, the controller can control the centrifugal fan in the detection space to increase the rotating speed so as to reduce the air pressure in the detection space, and the air in the detection space is prevented from flowing into the sample processing space, so that the protective performance of the equipment can be further improved.

Alternatively, as shown in fig. 3, the base frame 21 includes an upper supporting portion located at an upper portion of the base frame 21 and a lower supporting portion 211 located at a lower portion of the base frame 21 and used for supporting the upper supporting portion. The left and right side portions of the upper support portion are a table portion 212 and a holder portion 213, respectively, the upper portion of the table portion 212 defining a sample processing space, and the upper portion of the holder portion 213 defining a detection space. The sample cup separation module 22, the sample extraction module 23, and the system construction module 24 are provided in a sample processing space on the stage portion 212, and the detection module 25 is provided in a detection space on the holder portion 213. Therefore, the structure of the full-automatic molecular diagnosis all-in-one machine is more reasonable and compact. The base frame 21 is composed of aluminum profiles, a table top aluminum fine plate, a support column and the like, and mainly plays a role in supporting and fixing other functional modules.

In addition, the sample cup module 22 may include a sample tube sample introduction carrier 221, a sample tube scanner 222, a clamping unit 227, a first motion unit 225, a second motion unit 226, a consumable unit 228, and a Tip head discard bin 229. The sample tube code scanner 222 is used for inputting information to a detection sample, the clamping units 227 are arranged in pairs and used for clamping the sample tube filled with the detection sample, the moving ends of the first moving units 225 are provided with cover opening mechanical claws 223 in pairs and used for driving the cover opening mechanical claws 223 to transfer the sample tube to the clamping units 227 for cover opening, the moving ends of the second moving units 226 are provided with liquid transferring units 224 in pairs and used for driving the liquid transferring units 224 to suck the detection sample located in the clamping units 227 into the sample extraction module 23, the consumable units 228 are used for containing Tip head consumables, and the Tip head waste garbage cans 229 are used for containing waste Tip heads.

Specifically, as shown in fig. 5, the first and second movement units 225 and 226 are triaxial movement modules, which are disposed at the upper portion of the sample processing space and located at both sides of the sample processing space, respectively. The sample tube scanner 222, and the clamp unit 227 are disposed on the table portion 212 below the sample processing space and below the first moving unit 225. A consumable unit 228 is disposed on the table portion 212 below the second movement unit 226 and a Tip head waste bin 229 is disposed under the table portion 212. The sample tube sample injection carrier 221 is located between the sample tube scanner 222 and the clamping unit 227. Thus, the structure of the sample cup separating module 22 is more compact, and the detection efficiency is higher. The sample tube advances carrier 221 double setting, and clamping unit 227 sets up in pairs, uncaps manipulator claw 223 and sets up in pairs on the motion end of first motion unit 225, and pipetting pump unit 224 sets up in pairs on the motion end of second motion unit 226, so, can carry out two liang cooperation detection simultaneously, can further promote detection efficiency.

In addition, the sample tube sample carrier 221 can adopt pull-type sample loading, the sample tube code scanner 222 is placed on the left side edge of the sample tube sample carrier 221, and in the pushing process of the sample tube sample carrier 221, the sample tube code scanner 222 captures sample tube information to complete the code scanning function. The cover opening mechanical gripper 223 is driven by the first movement unit 225 to move the cover opening mechanical gripper 223 along the X axis, the Y axis and the Z axis to the position of the designated sample tube, so as to clamp the sample tube. The clamping unit 227 mainly realizes clamping of the sample tube in the process of opening and closing the cover, and adopts flexible fingers to realize clamping of different sample tubes. The pipetting unit 224 adopts a high-precision pipetting pump, and is driven by the second motion unit 226 to realize the motion of the X axis, the Y axis and the Z axis, move to a designated position, press the Tip head at the position of the consumable unit 228, realize the suction and the discharge of sample liquid, and withdraw the waste Tip head at the position of the Tip head waste dustbin 229. In the working process, after the sample tube sample introduction carrier 221 moves to a designated position, the cover opening mechanical gripper 223 moves to the designated position of the sample tube through XYZ three axes, the mechanical gripper is opened to clamp the sample tube, the sample tube is lifted to move to the position of the clamping unit 227, the clamping module acts to clamp the sample tube, the cover opening mechanical gripper 223 rotates to open the sample tube cover, meanwhile, the liquid transfer pump moves to the position of the consumable unit 228 under the driving of the second moving unit 226 to press the Tip head, then moves to the clamping unit 227, absorbs sample liquid, moves to the sample extraction module 23 to finish liquid injection, and after liquid injection is finished, the liquid transfer pump moves to the Tip waste garbage can 229 to finish falling into the garbage can, so that the sample cup separating function is finished.

The sample extraction module 23 adopts a method of adsorbing magnetic beads on the sample extraction module and a method of moving magnetic rods to extract nucleic acid, has the advantages of high speed, simple structure and the like, and can reasonably design the distance between the magnetic rods to prevent pollution in the extraction process. Reagents for extraction such as lysate, washing solution and the like are packaged in the pre-packaged 96-hole deep-hole plate, the sample solution which is completed by the separation cup is injected into the sample hole of the deep-hole plate, the deep-hole plate is pushed into the module, the motor drives the magnetic rod/magnetic rod sleeve to move up and down and left and right, and the nucleic acid is cracked, purified and the like through repeated combination and separation of the magnetic rod and the nucleic acid.

Alternatively, the system construction module 24 may include a detection reagent unit 241, a detection reagent cap unit 242, a reaction tube unit 243, and a reaction tube movement unit 244. The detection reagent unit 241 is used for accommodating a detection reagent tube, the detection reagent uncapping unit 242 is used for uncapping the detection reagent tube, the reaction tube unit 243 is used for accommodating a reaction tube, the second movement unit 226 is also used for driving the pipetting unit 224 to inject the extract in the detection reagent tube into the reaction tube, and the reaction tube movement unit 244 is used for transferring the reaction tube filled with the extract into the detection module 25.

Specifically, as shown in fig. 6, a detection reagent unit 241, a detection reagent uncap unit 242, a reaction tube unit 243, and a reaction tube moving unit 244 are disposed at a lower portion of the sample processing space, the detection reagent unit 241 is located below the detection reagent uncap unit 242, the reaction tube unit 243 and the reaction tube moving unit 244 are located at a side of the sample processing space near the detection module 25, and the reaction tube unit 243 is disposed on the reaction tube moving unit 244. The main function of the system construction module 24 is to inject the purified nucleic acid sample into the PCR reaction tube through the liquid transferring pump unit 224, the detection reagent cover opening unit 242 presses and lifts the amplification reagent tube cover, the amplification reagent tube cover is discarded to the Tip waste dustbin 229, the amplification reagent tube cover opening is completed, meanwhile, the liquid transferring pump unit 224 moves to the position of the consumable unit 228 to press and pick the Tip, then moves to the detection reagent unit 241 to absorb the amplification reagent, the PCR reaction tube unit 243 injects the amplification reagent to blow and mix uniformly, and the waste Tip is discarded to the position of the Tip waste dustbin 229.

Further, the fully automatic molecular diagnosis all-in-one apparatus further includes a detection module housing 256 provided on the base frame 21 and defining a detection space, the detection module housing 256 being provided with a detection module side opening 2561 for receiving the reaction tube containing the extract, the detection module further including a reaction tube reject tub unit 254 and a third movement unit 253 provided at a movement end thereof with a reaction tube gripper 252, the third movement unit 253 for driving the reaction tube gripper 252 to transfer the reaction tube containing the extract into the detection chamber 251.

Still further, the detection module may further include a detection module door mechanism 255, the detection module door mechanism 255 being disposed on the detection module housing 256 and configured to open and close the detection module side opening 2561.

Specifically, as shown in fig. 7 to 12 and 14, the third moving unit 253 is provided at an upper portion of the detection module housing 256, the detection compartment 251 is located at a lower portion of the detection module housing 256, the reaction tube moving unit 244 extends from the detection module side opening 2561 into the detection module 25, and the reaction tube discarding tub unit 254 is located at a front portion of the detection module 25. The detection module 25 mainly realizes the functions of clamping and moving the amplification reaction tube, amplifying sample nucleic acid, analyzing data and discarding the amplification reaction tube. The detection module 25 adopts an independent sealed space design, so that amplified products are left in the design space to the greatest extent, and no pollution is caused to other positions of the instrument. In the working process, the detection module cabin door mechanism 255 is opened, the reaction tube moving unit 244 moves the PCR amplification tube to the designated position of the detection module 25, the reaction tube gripper 252 is driven by the third moving unit 253 to move to the designated position, the PCR reaction tube is clamped, the reaction tube is moved to the detection cabin 251, and after the completion, the PCR amplification module cabin door is closed. After the amplification reaction is completed, the PCR reaction tube is discarded into the reaction tube discarding barrel unit 254, and the amplification reaction is completed.

Optionally, the detection module cabin door mechanism 255 includes a cover plate, a driving motor, a pivot shaft and a screw block, the driving motor is disposed on a mounting plate formed with the detection module side opening 2561, the pivot shaft is in driving connection with the driving motor, the screw block is threaded on the pivot shaft and is in threaded connection with the pivot shaft, the cover plate is fixed with the screw block, and the screw block moves along the axial direction of the pivot shaft with the cover plate under the driving action of the driving motor so as to open or close the detection module side opening 2561. Further, the side opening 2561 of the detection module is only opened when the detection flat tube enters the detection module 25, and the rest of the time is closed, so that the time for exchanging air inside the detection module housing 256 and air outside the detection module housing 256 is reduced, the tightness of the inside of the detection module housing 256 and the inside of the detection cabin 251 is further enhanced, and the possibility of pollution is further reduced.

Optionally, the detection pod 251 may include a pod 2511, a detection unit, and a pod cover mechanism 2513. The housing 2511 is provided with a housing opening 2512, and a detection unit is provided in the housing 2511 for detecting the extract in the reaction tube, and a housing cover mechanism 2513 is provided on the housing 2511 for opening and closing the housing opening 2512. As shown in fig. 10 and 12, the cabin 2511 is located at the bottom of the detection space, and the cabin opening 2512 is located at the top of the cabin 2511.

In addition, the detection module 25 may further include a negative pressure chamber 257 provided at one side of the chamber 2511 and communicating with the inside of the chamber 2511, and a detection module centrifugal fan (not shown) for drawing out the inside air of the chamber 2511 is provided in the negative pressure chamber 257. As shown in fig. 10, the negative pressure compartment 257 is located at a lower portion of the detection module housing 256.

Specifically, the side wall of the detection cabin 251 far away from the negative pressure cabin 257 is provided with a plurality of air inlets, when the centrifugal fan rotates, air in the detection cabin 251 can be pumped into the negative pressure cabin 257 and then discharged from the negative pressure cabin 257, so that a negative pressure detection environment is formed in the detection cabin 251, and detection liquid or pollutants in the detection cabin 251 can be further prevented from overflowing to the outside of the detection cabin 251 and polluting the outside of the detection cabin 251. Further, the number of negative pressure tanks 257 is identical to the number of detection tanks 251, and if there are a plurality of detection tanks 251, the number of negative pressure tanks 257 correspondingly increases.

In addition, as shown in fig. 7 and 11, the bottom wall of the negative pressure chamber 257 may be provided with a negative pressure chamber air outlet 2571, the bottom wall of the detection module housing 256 is provided with a first air pipe penetrating opening 2562, the top wall of the detection module housing 256 is provided with a second air pipe penetrating opening 2563, the detection module 25 further includes an exhaust pipe (not shown in the drawings) with one end communicating with the negative pressure chamber air outlet 2571 from the bottom wall of the negative pressure chamber 257, and the other end of the exhaust pipe sequentially penetrates through the first air pipe penetrating opening 2562 and the second air pipe penetrating opening 2563 to exhaust the air outside the detection module housing 256.

Specifically, a filter (not shown in the drawing) is disposed at the second air pipe penetrating opening 2563, and the side surface of the negative pressure cabin 257 is communicated with the cabin body side opening 2514 on the detection cabin 251, that is, the air in the detection cabin 251 enters the negative pressure cabin 257 from the side surface of the negative pressure cabin 257 and flows out of the negative pressure cabin 257 from the bottom of the negative pressure cabin 257, the air flowing out of the negative pressure cabin 257 passes through the exhaust pipe and flows out of the detection module 25 after being filtered, so that the air flowing out of the detection cabin 251 is prevented from carrying pollutants and polluting the air outside the amplification device. Further, the exhaust pipe is arranged in the detection module shell, so that reasonable arrangement of the trend of the exhaust pipe is realized; when the number of the detecting chambers 251 and the negative pressure chambers 257 increases, the number of the first air pipe penetrating openings 2562, the second air pipe penetrating openings 2563 and the exhaust pipes correspondingly increases, so as to avoid cross contamination of the air flowing out of different detecting chambers 251.

Further, as shown in fig. 8 and 13, the reaction tube waste barrel unit 254 may include a waste barrel mounting seat 2541 having a barrel cavity therein and a barrel body 2542 extendably disposed in the barrel cavity, the front side wall of the detection module housing 256 is provided with a detection module front opening 2564 disposed in alignment with the barrel body extension opening of the waste barrel mounting seat 2541, and the barrel body 2542 can extend from the detection module front opening 2564 or close the detection module front opening 2564.

Still further, as shown in fig. 13, the top of the waste tub mounting base 2541 may be provided with a mounting base top opening, and the reaction tube waste tub unit 254 further includes a mounting base cover mechanism 2543, and the mounting base cover mechanism 2543 is disposed on the waste tub mounting base 2541 and is used for opening and closing the mounting base top opening.

Specifically, the reaction tube waste tub unit 254 is disposed at a position near the mounting plate formed with the detection module front opening 2564, and the tub 2542 may be laterally inserted into the tub cavity through the detection module front opening 2564 so as to be put into the empty tub 2542 or take out the full tub 2542; the mount cover mechanism 2543 includes a cover plate, a driving motor, a pivot shaft and a screw block, wherein the driving motor is arranged at the top of the waste barrel mount 2541, the pivot shaft is in driving connection with the driving motor, the screw block is arranged on the pivot shaft in a penetrating manner and is in threaded connection with the pivot shaft, the cover plate is fixed with the screw block, and the screw block moves along the axial direction of the pivot shaft along the cover plate under the driving action of the driving motor so as to open or close the top opening of the mount. Further, the top opening of the mounting seat is only opened when the detected flat detection pipe is placed in the barrel 2542, and the rest time is closed, so that the pollution to the inside of the detection module 25 caused by overflow of detection liquid is avoided.

In one embodiment of the present utility model, the detection chamber 251 further includes a sterilizing lamp disposed in the detection module housing 256, where the sterilizing lamp may be an ultraviolet lamp, and may be turned on after the detection of the detection liquid is completed, so as to perform irradiation sterilization on the interior of the detection module housing 256, thereby avoiding the contamination remaining during the previous detection and the contamination of the detection liquid in the subsequent detection operation, reducing the occurrence of contamination situations, and further facilitating the improvement of the accuracy of the detection result.

In one embodiment of the present utility model, as shown in fig. 14, the third moving unit 253 includes an X-axis moving module 2531, a Y-axis moving module 2532, a Z-axis moving module 2533, and a reaction tube jaw 252 for clamping a detection flat tube, the Y-axis moving module 2532 being laterally movably disposed on the X-axis moving module 2531, the Z-axis moving module 2533 being longitudinally movably disposed on the Y-axis moving module 2532, the reaction tube jaw 252 being vertically movably disposed on the Z-axis moving module 2533. Specifically, the X-axis motion module 2531 includes a first guide rail, a first slider, a synchronous belt assembly, a ball screw assembly and a driving motor, where the first guide rail is arranged along a transverse direction, the first slider is movably disposed on the first guide rail, the driving motor is in driving connection with the first slider through the ball screw assembly, and the above arrangement can effectively improve the precision of transverse movement due to a longer transverse movement stroke, and the synchronous belt assembly is adopted between the driving motor and the ball screw assembly for transmission; the Y-axis motion module 2532 includes a second guide rail, a second slider, and a driving motor, where the second guide rail is longitudinally arranged and fixed with the first slider, so that the X-axis motion module 2531 carries the Y-axis motion module 2532, the Z-axis motion module 2533, and the reaction tube clamping jaw 252 to move laterally, the second slider is movably disposed on the second guide rail, and the driving motor is in driving connection with the second slider through a conveyor belt assembly, which is beneficial to reducing the manufacturing cost; the Z-axis moving module 2533 includes a third guide rail, a third slider, and a driving motor, where the third guide rail is vertically arranged and fixed with the second slider, so that the Y-axis moving module 2532 moves laterally with the Z-axis moving module 2533 and the reaction tube clamping jaw 252, the third slider is movably disposed on the third guide rail, the driving motor is in driving connection with the third slider, and the reaction tube clamping jaw 252 and the third slider are fixed, so that the Z-axis moving module 2533 moves vertically with the reaction tube clamping jaw 252, where the driving motor is optionally a linear screw motor due to a constant load during the vertical movement. The above components cooperate to achieve 5 degrees of freedom of movement such that the reactor tube gripper 252 transfers the clamped flat tube to various locations within the detection module 25 (e.g., the detection chamber 251, the reactor tube reject bin unit 254, etc.).

Further, the reaction tube clamping jaw 252 in this embodiment includes a first clamping jaw and a second clamping jaw that can be opened and closed, and flexible clamping layers made of flexible materials are respectively disposed on the clamping surfaces of the first clamping jaw and the second clamping jaw, so that the first clamping jaw and the second clamping jaw have a certain elasticity, and can clamp objects with different sizes, thereby further expanding the application range of the amplification device.

In the working process, the automatic molecular diagnosis integrated machine is powered on, and the equipment starts working by pressing a start button. And opening the consumable material access cabin door 36, extracting the consumable material drawer, placing the deep extraction pore plate, the Tip consumable material and the amplification reagent into a designated position, and closing the consumable material access cabin door 36. Opening the sample tube access cabin door 35, extracting the sample tube carrier, inserting the sample tube into the sample tube carrier, pushing the sample tube carrier into the appointed position, closing the sample tube access cabin door 35, clicking the start button, starting the equipment to work, extracting the sample tube for sample or discarding after the work is completed, discarding the discarded deep pore plate, the Tip head consumable frame and the amplified reagent tube into the medical waste bag, sealing the discarded Tip head waste bag and the PCR reaction tube waste bag and discarding the waste bag into the appointed position of the medical waste, disinfecting the table top, and opening the ultraviolet lamp for disinfection, thereby completing the single test.

In summary, the full-automatic molecular diagnosis all-in-one machine provided by the utility model realizes the functions of original tube sample injection of a sample tube, automatic cup separation of a sample liquid, nucleic acid extraction by an upper adsorption magnetic bead method, reaction system construction, PCR nucleic acid amplification, background data analysis, result uploading and auditing and the like, realizes full automation of a nucleic acid detection process, reduces sample detection waiting time along with detection, and provides powerful guarantee for molecular diagnosis.

The foregoing details of the optional implementation of the embodiment of the present utility model have been described in conjunction with the accompanying drawings, but the embodiment of the present utility model is not limited to the specific details of the foregoing implementation, and various simple modifications may be made to the technical solution of the embodiment of the present utility model within the scope of the technical concept of the embodiment of the present utility model, where all the simple modifications belong to the protection scope of the embodiment of the present utility model.

In addition, the specific features described in the foregoing embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.

In addition, any combination of various embodiments of the present utility model may be performed, so long as the concept of the embodiments of the present utility model is not violated, and the disclosure of the embodiments of the present utility model should also be considered.

Claims (15)

1. Full-automatic molecular diagnosis all-in-one equipment, its characterized in that, full-automatic molecular diagnosis all-in-one equipment includes:

A base frame (21) provided with a sample processing space and a detection space;

A sample cup separating module (22) which is positioned in the sample processing space and is used for inputting information and separating cups for detection samples;

A sample extraction module (23) which is positioned in the sample processing space and is used for extracting the sample to be detected after the cup separation;

A system construction module (24) located in the sample processing space, the system construction module (24) being configured to process the extract and send the processed extract out; and

And a detection module (25) positioned in the detection space and comprising a detection chamber (251), wherein the detection chamber (251) is used for receiving the processed extract and performing sealing detection.

2. The fully automatic molecular diagnostic all-in-one machine according to claim 1, wherein the detection module (25) is provided with a plurality of detection tanks (251) which are arranged side by side and can be independently detected, and each detection tank (251) is provided with a plurality of detection bits.

3. The fully automated molecular diagnostic all-in-one machine apparatus of claim 1, wherein the sample cup separation module (22) comprises:

the sample tube code scanner (222) is used for inputting information to the detection sample;

Clamping units (227) arranged in pairs for clamping a sample tube containing a test sample;

A first moving unit (225) with a pair of cover opening mechanical claws (223) at the moving end and used for driving the cover opening mechanical claws (223) to transfer the sample tube to the clamping unit (227) for cover opening; and

And a second moving unit (226) having a pair of moving ends provided with a pipetting unit (224) and for driving the pipetting unit (224) to aspirate the test sample located in the gripping unit (227) into the sample extraction module (23).

4. The fully automatic molecular diagnostic all-in-one machine device according to claim 1, wherein the sample cup module (22) comprises a sample tube sample carrier (221) arranged in double rows;

And/or, the sample cup separation module (22) comprises a consumable unit (228) for containing Tip head consumables and a Tip head waste dustbin (229).

5. A fully automated molecular diagnostic all-in-one machine according to claim 3, wherein the system building block (24) comprises:

A detection reagent unit (241) for accommodating a detection reagent tube;

A detection reagent uncapping unit (242) for uncapping the detection reagent tube;

A reaction tube unit (243) for accommodating a reaction tube, the second movement unit (226) further for driving the pipetting unit (224) to inject the extract in the detection reagent tube into the reaction tube; and

A reaction tube movement unit (244) for transferring the reaction tube containing the extract into the detection module (25).

6. The full-automatic molecular diagnosis all-in-one machine according to claim 1, further comprising a closed machine body casing (3), wherein the base frame (21) is arranged in the machine body casing (3), the machine body casing (3) comprises a casing sheet metal (31), an observation window (35), a touch screen (37), a sample tube access cabin door (32), a consumable access cabin door (33) and a reaction tube access cabin door (34), wherein the observation window (35), the reaction tube access cabin door (34), the sample tube access cabin door (32) and the consumable access cabin door (33) are all arranged on the front end face of the casing sheet metal (31) aligned with the sample processing space, and the touch screen (37) is arranged on the front end face of the casing sheet metal (31) aligned with the detection space.

7. The fully automatic molecular diagnostic all-in-one machine according to claim 6, further comprising a sample processing space laminar flow system (36) provided on the machine body casing (3), the sample processing space laminar flow system (36) being configured to form a negative pressure inside the machine body casing (3) to prevent an unfiltered outflow of an internal contaminated air flow, the sample processing space laminar flow system (36) comprising a sample processing space centrifugal fan and a sample processing space filter provided at an air inlet of the sample processing space centrifugal fan.

8. The fully automated molecular diagnostic all-in-one machine according to claim 1, wherein the base frame (21) comprises:

An upper support part which is positioned at the upper part of the base frame (21) and is provided with a table top part (212) and a bracket part (213) at the left and right sides, wherein the table top part (212) is used for limiting the sample processing space, and the bracket part (213) is used for limiting the detection space; and

And a lower support portion (211) which is positioned at the lower portion of the base frame (21) and is used for supporting the upper support portion.

9. The fully automated molecular diagnostic all-in-one machine according to any one of claims 1 to 8, further comprising a detection module housing (256) on the base frame (21) and defining the detection space, the detection module housing (256) being provided with a detection module side opening (2561) for receiving a reaction tube filled with an extract, the detection module further comprising a third movement unit (253) provided with a reaction tube gripper (252) at a movement end, the third movement unit (253) being for driving the reaction tube gripper (252) to transfer a reaction tube filled with an extract into the detection compartment (251).

10. The fully automated molecular diagnostic all-in-one machine of claim 9, wherein the detection module further comprises a detection module door mechanism (255), the detection module door mechanism (255) being disposed on the detection module housing (256) and configured to open and close the detection module side opening (2561).

11. The fully automated molecular diagnostic all-in-one machine of claim 9, wherein the detection pod (251) comprises:

A cabin body (2511) located at the bottom of the detection space and provided with a cabin body opening (2512), wherein the cabin body opening (2512) is located at the top of the cabin body (2511);

A detection unit provided in the capsule (2511) and configured to detect an extract in the reaction tube; and

A hatch mechanism (2513) disposed on the hatch (2511) and configured to open and close the hatch opening (2512).

12. The fully automatic molecular diagnostic all-in-one machine according to claim 11, wherein the detection module (25) further comprises a negative pressure cabin (257) arranged on one side of the cabin body (2511) and communicated with the interior of the cabin body (2511), and a detection module centrifugal fan for pumping out the internal air of the cabin body (2511) is arranged in the negative pressure cabin (257).

13. The full-automatic molecular diagnosis all-in-one machine according to claim 12, wherein a bottom wall of the negative pressure cabin (257) is provided with a negative pressure cabin air outlet (2571), a bottom wall of the detection module housing (256) is provided with a first air pipe penetrating opening (2562), a top wall of the detection module housing (256) is provided with a second air pipe penetrating opening (2563), the detection module (25) further comprises an exhaust pipe, one end of which is communicated with the negative pressure cabin air outlet (2571) from the bottom wall of the negative pressure cabin (257), and the other end of the exhaust pipe sequentially penetrates through the first air pipe penetrating opening (2562) and the second air pipe penetrating opening (2563) to exhaust air outside the detection module housing (256).

14. The fully automatic molecular diagnostic all-in-one machine according to claim 9, wherein the detection module (25) further comprises a reaction tube waste barrel unit (254) located at the front, the reaction tube waste barrel unit (254) comprises a waste barrel mounting seat (2541) with a barrel cavity therein and a barrel body (2542) extendably arranged in the barrel cavity, the front side wall of the detection module housing (256) is provided with a detection module front opening (2564) arranged in alignment with a barrel body extension opening of the waste barrel mounting seat (2541), and the barrel body (2542) can extend from the detection module front opening (2564) or close the detection module front opening (2564).

15. The fully automated molecular diagnostic all-in-one machine of claim 14, wherein a top of the waste bucket mounting block (2541) is provided with a mounting block top opening, the reaction tube waste bucket unit (254) further comprises a mounting block cover mechanism (2543), and the mounting block cover mechanism (2543) is disposed on the waste bucket mounting block (2541) and is configured to open and close the mounting block top opening.