CN112920948A - Nucleic acid detection pretreatment instrument - Google Patents
Nucleic acid detection pretreatment instrument Download PDFInfo
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- CN112920948A CN112920948A CN202110355141.5A CN202110355141A CN112920948A CN 112920948 A CN112920948 A CN 112920948A CN 202110355141 A CN202110355141 A CN 202110355141A CN 112920948 A CN112920948 A CN 112920948A
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- acid detection
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 44
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 44
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 44
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 239000011324 bead Substances 0.000 claims abstract description 33
- 238000006073 displacement reaction Methods 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 239000011148 porous material Substances 0.000 claims abstract description 8
- 238000005406 washing Methods 0.000 claims description 18
- 238000012545 processing Methods 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 14
- 239000002699 waste material Substances 0.000 claims description 13
- 239000003480 eluent Substances 0.000 claims description 8
- 239000006166 lysate Substances 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims 1
- 238000007781 pre-processing Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 abstract description 84
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- 238000012546 transfer Methods 0.000 abstract description 3
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- 238000010438 heat treatment Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention relates to a pretreatment instrument for nucleic acid detection, which is mainly used for extracting nucleic acid in a biological sample and belongs to the technical field of nucleic acid detection. This treater, including sample pipe, 96 orifice plates and the eight ally oneself with the pipe of arranging in advance in the treater, still including installing in the treater: the screwing device is used for clamping the sample tube and loosening or screwing the sample tube cap; the liquid transfer device is used for carrying and transferring the sample; the magnetic rod displacement device is used for adsorbing sample nucleic acid and magnetic beads and realizing the extraction operation of the sample in the 96-pore plate; and the eight-pipe receiving device is used for receiving the extracted sample to be detected. The product can replace all manual operations in the prior art through a full-automatic instrument, and the requirement on the professional degree of an operator is greatly reduced through one-key operation through a preset program.
Description
Technical Field
The invention relates to a pretreatment instrument for nucleic acid detection, which is mainly used for extracting nucleic acid in a biological sample and belongs to the technical field of nucleic acid detection.
Background
In the prior art, before nucleic acid detection, a nucleic acid extraction treatment needs to be carried out on an original sample (such as a throat swab, alveolar lavage fluid, blood and the like). At present, the bead method is common in clinical application, namely, an original sample is firstly sucked out from an original storage tube, added into a lysis solution, uniformly mixed by oscillation, and the lysis solution is utilized to destroy the cell structure of a target in the original sample, so that nucleic acid in cells is released; then adding magnetic beads subjected to surface group treatment to adsorb nucleic acid, adsorbing the magnetic beads adhered with the nucleic acid by using a magnet, and transferring the magnetic beads into washing solution 1; removing the magnet to suspend the magnetic beads in the washing solution, adsorbing the magnetic beads with the nucleic acid by using the magnet again, and transferring the magnetic beads into the washing solution 2; then discarding the washing solution 2, volatilizing the magnetic beads adhered with the nucleic acid to dry the surface liquid, and transferring the magnetic beads to the eluent; the magnet is removed and the beads are suspended in the eluent, whereupon the nucleic acids dissociate from the beads, are released from the beads, and the beads are attracted away by the magnet, leaving the eluent containing the extracted nucleic acids. The eluate is used as an upper machine sample, added into the prepared detection solution, loaded into a special PCR tube, covered with a tube cover, and placed into a PCR instrument for amplification detection (PCR: Polymerase Chain Reaction, Chinese Polymerase Chain Reaction). The whole pretreatment process is complex in operation, needs professional technicians and usually needs 2-3 hours of operation time.
It can be seen that the main drawbacks of the prior art are:
(1) the whole pretreatment process is complex in operation and needs professional technicians; (2) even if a nucleic acid extractor is used, the processes of cracking, washing and elution are only replaced, and the processes of sucking an original sample, sucking a sample on a machine, opening and closing a cover of an original storage tube and covering a PCR tube are all completed manually; (3) the total treatment time is long, generally 2 to 3 hours, and even if a nucleic acid extractor is used, 1 to 2 hours; (4) if the original sample is an infectious sample, the process of uncapping and sampling increases the propagation risk and is unfavorable for operators.
Disclosure of Invention
The invention aims to provide a nucleic acid detection pretreatment instrument which is simple and convenient to operate and high in working efficiency.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: the utility model provides a processing apparatus before nucleic acid testing, includes sample pipe, 96 orifice plates and the octal union coupling pipe of arranging in the processing apparatus in advance, still including installing in the processing apparatus:
the cover screwing device is arranged beside the sample tube and is used for clamping the sample tube and loosening or screwing the sample tube cover;
the pipettor is arranged above the inside of the treatment instrument and used for carrying and transferring the sample;
the magnetic rod displacement device is arranged beside the 96-pore plate and is used for adsorbing nucleic acid and magnetic beads of a sample to realize the extraction operation of the sample in the 96-pore plate;
and the eight-pipe receiving device is arranged in the processing instrument and below the processing instrument and is used for receiving the extracted sample to be detected.
Preferably, the screw-capping device comprises a clamp and a rotating head, the clamp clamps the sample tube and drives the sample tube cap to rotate by the rotating head, and the sample tube cap is loosened or screwed.
Preferably, the clamp moves in the X-axis direction and the Y-axis direction to realize switching between different tubes; the rotating head moves in the X-axis direction, the Y-axis direction and the Z-axis direction, and switching among different pipes is realized.
Preferably, the pipette device further comprises a sucker and a waste discharge port, and the pipette device moves in the X-axis direction, the Y-axis direction and the Z-axis direction so as to pick up different suckers and suck a sample through the sucker; the treater be equipped with the waste discharge mouth, the suction head after the use is abandoned through the waste discharge mouth.
Preferably, the magnetic rod displacement device comprises a displacement magnetic rod and a magnetic rod sleeve, the displacement magnetic rod is inserted into the magnetic rod sleeve, the displacement magnetic rod adsorbs magnetic beads with samples through displacement, and extraction operations including washing, airing and elution are carried out.
Preferably, the eight-joint pipe receiving device comprises an eight-joint pipe frame, a cover taking device and a sliding track, the extracted sample is transferred into the eight-joint pipe of the eight-joint pipe frame through a liquid transfer device, and the cover taking device tightly covers the eight-joint pipe; the eight-linked pipe frame is connected in a sliding mode along the sliding track.
Preferably, the device also comprises a temperature controller, and the temperature controller is arranged in the processor.
Preferably, the treatment instrument further comprises an air filtering system, and the treatment instrument is internally provided with the air filtering system.
Preferably, the 96-well plate pre-dispensing comprises: column 1 and column 4 are lysates; column 2 and column 5 are washing solution 1; columns 3 and 6 are magnetic beads; column 8 and column 11 are washing solution 2; the eluent is shown in columns 9 and 12.
Preferably, the air filtering system comprises an air inlet fan, an air inlet filter, an air outlet fan and an air outlet filter, wherein the air inlet fan and the air inlet filter are arranged at the upper part of the treatment instrument, and the air outlet fan and the air outlet filter are arranged at the lower part of the treatment instrument.
Compared with the prior art, the invention has the following advantages and effects:
1. the sample tube is clamped by the clamp, and the motor is utilized to drive the rotating head, so that automatic screwing is realized. The rotary head is gradually pushed up or down during the screwing process, so that the sample tube cap is completely opened or closed. The pipette is matched with the sucker, so that a liquid sample with a set volume can be sucked. Therefore, automatic sampling of the original sample is realized, and manual operation is replaced.
2. After each sample tube is sampled, the sample tube cap can be retightened, reducing the risk of leakage and the possibility of cross-contamination.
3. The suction head after being used is automatically discharged out of the instrument through the waste discharge port, so that the pollution risk is reduced.
4. The magnetic rod and the magnetic rod sleeve are matched for application, so that the automation of the nucleic acid extraction step can be realized, and the function of the magnetic rod is consistent with that of a common nucleic acid extractor.
5. Pipettes, in conjunction with tips, can aspirate a set volume of liquid sample in a set well site in a 96-well plate. Realize the automation of nucleic acid sample application.
6. After the sample adding of the eight-linkage tube is completed, the eight-linkage tube cover can be clamped by the cover taking device and can be automatically covered tightly, so that the automation of the eight-linkage tube cover adding is realized.
7. The integration cooperation of steps such as original sample, nucleic acid extraction, nucleic acid sample application of sample and eight ally oneself with pipe capping has promoted overall degree of automation greatly, has improved operating efficiency.
8. The instrument adopts the mode of upper air inlet and lower air outlet to form vertical air flow, so that the cross contamination among samples can be reduced. And air inlet and air exhaust are filtered by sampling HEPA, so that external air does not pollute samples and reagents, and internal air does not pollute the environment and operators. The safety of the whole operation steps is greatly improved.
9. The eight-connection pipe frame and the eight-connection pipe cover frame are integrally drawn and pulled in a rail type mode, and the eight-connection pipe cover are conveniently loaded before operation.
The bottom of the 10.96 pore plate is provided with a completely attached metal module (temperature controller), which can independently control the temperature rise and fall of a single column to realize the heating of specific solution.
Drawings
Fig. 1 is a front structural view of the product.
Fig. 2 is a schematic view of a portion of fig. 1.
Fig. 3 is a side view of a portion of the present product.
Fig. 4 is a top view of a portion of the present product.
FIG. 5 is a schematic diagram of the corresponding well site of the 96-well plate reagent in the product.
Fig. 6 is a main circuit diagram of the product.
Fig. 7 is a block connection line diagram of the present product.
In the figure: 1. rotating the head; 2. a clamp; 3. a pipettor; 4. a displacement magnetic rod; 5. a cap remover; 6. a sample rack; 7. a suction head box A; 8. a 96-well plate; 9. an eight-connected pipe frame; 10. eight-connected pipe cover frame; 11. a sample tube; 111. a sample tube cap; 12. a sliding track; 13. a suction head; 14. a magnetic rod sleeve; 15. a head cartridge B; 16. a waste discharge port; 17. a Y-direction guide rail; 18. an X-direction guide rail; 19. an air intake fan; 20. filtering the inlet air; 21. an exhaust fan; 22. filtering the exhaust gas; 23. and (7) a temperature controller.
Detailed Description
The invention is further illustrated by the following specific examples.
The design concept is as follows: the whole instrument motion part is four functional modules, is switch lid module, moves liquid module, bar magnet module and adds the lid module respectively, and the instrument is equipped with temperature control module in the function simultaneously, can adjust the temperature of pretreatment in-process to reach better treatment. In addition, the instrument is provided with an upper efficient air filtering system and a lower efficient air filtering system, so that the air entering the instrument does not bring external pollution to influence a test result, the air discharged from the instrument does not pollute the use environment, and the user is safer.
The product of this embodiment is implemented by running a corresponding program through the existing control chip, as shown in fig. 6 and 7, a PLC chip is taken as an example, and since there are many implementation manners and connection manners, detailed description is omitted, and all that can implement the same or equivalent functions of this product falls into the protection scope.
The first embodiment is as follows:
as shown in fig. 1 to 5, the product is a nucleic acid detection pretreatment instrument, which comprises a sample tube 11, a 96-well plate 8 and an octal tube, which are pre-arranged in the pretreatment instrument, and further comprises: a screw-capping device, provided beside the sample tube 11, for clamping the sample tube 11 and loosening or screwing the sample tube cap 111; the pipettor 3 is arranged above the inside of the treatment instrument and used for carrying and transferring the sample; the magnetic rod displacement device is arranged beside the 96-pore plate and is used for adsorbing nucleic acid and magnetic beads of the sample to realize the extraction operation of the sample in the 96-pore plate 8; and the eight-pipe receiving device is arranged in the processing instrument and below the processing instrument and is used for receiving the extracted sample to be detected.
More specifically, the screw capping device of the present embodiment includes a clamp 2 and a rotary head 1, wherein the clamp 2 clamps the sample tube 11 and drives the sample tube cap 111 to rotate by the rotary head 1, thereby loosening or screwing the sample tube cap 111.
The gripper 2 of the present embodiment moves in the X-axis direction and the Y-axis direction to switch between different tubes 11, and the Y-direction guide rail 17 and the X-direction guide rail 18 in the present embodiment play a guiding role, and similarly, the rotary head 1 moves in the X-axis direction, the Y-axis direction, and the Z-axis direction to switch between different tubes 11.
The embodiment also comprises a sucker 13 and a waste discharge port 16, and the pipettor 3 moves in the X-axis direction, the Y-axis direction and the Z-axis direction, so that different suckers 13 are taken out and a sample is sucked by the suckers 13; the processor is provided with a waste discharge port 16, and the used suction head 13 is discarded through the waste discharge port 16.
The magnetic rod displacement device of this embodiment includes displacement magnetic rod 4 and magnetic rod cover 14, and displacement magnetic rod 4 inserts magnetic rod cover 14, and displacement magnetic rod 4 adsorbs the magnetic bead that has the sample through the displacement, carries out the extraction operation including washing, dries and elution.
The eight-joint pipe receiving device of the embodiment comprises an eight-joint pipe frame 9, a cover taking device 5 and a sliding track 12, wherein the extracted sample is transferred into the eight-joint pipe of the eight-joint pipe frame 9 through a liquid transferring device 3, and the cover taking device 5 tightly covers the eight-joint pipe; the octuple pipe frame 9 is connected in a sliding mode along a sliding rail 12.
The present embodiment further includes a temperature controller 23 and an air filtration system, the temperature controller 23 and the air filtration system are disposed in the processing instrument, wherein the air filtration system includes an air intake fan 19, an air intake filter 20, an air exhaust fan 21 and an air exhaust filter 22, the air intake fan 19 and the air intake filter 20 are disposed on the upper portion of the processing instrument, and the air exhaust fan 21 and the air exhaust filter 22 are disposed on the lower portion of the processing instrument.
The pre-dispensing of the 96-well plate 8 of the present embodiment comprises: column 1 and column 4 are lysis solutions, and column 2 and column 5 are washing solution 1; columns 3 and 6 are magnetic beads; column 8 and column 11 are washing solution 2; the eluent is shown in columns 9 and 12.
The working process is as follows:
first, the sample tube 11 with the clinical sample collected is loaded on the sample rack 6, and 16 samples can be loaded at a time. The 96-well plate 8 preloaded with the nucleic acid extraction reagent was loaded to the position of the 96-well plate 8 of FIG. 1 after the sealing film was peeled off. The octupled tube, which is preloaded with the detection liquid, is loaded at the position of the octupled tube holder 9 of fig. 1, and the octupled tube cap is loaded at the position of the octupled tube cap 10 of fig. 1.
The clamp 2 can clamp the sample tube 11 under the action of the motor, so that the sample tube cannot rotate. The clamp 2 can move in the X-axis direction and the Y-axis direction, thereby realizing the function of switching between different tubes.
The rotating head 1 drives the sample tube cap to rotate under the action of the rotating motor, and the sample tube cap is loosened or screwed down. The rotary head 1 integral module can move in the X-axis direction, the Y-axis direction and the Z-axis direction, thereby realizing the function of switching among different pipes.
After the rotary head 1 releases the cap of the sample tube 11, the pipette 3 moves down to insert a tip from the tip cassette a-7, as shown in fig. 2, in which the pipette 3 and the tip 13 are attached. The pipettor 3 can move in the X-axis direction, the Y-axis direction, and the Z-axis direction, thereby accurately extracting different tips.
The pipette 3 moves together with the tip 13 to above the sample tube with the tube cap opened, and then moves down so that the tip 13 is submerged below the liquid level in the sample tube, and the pipette 3 sucks 200uL of sample by the pump.
The pipetter 3 carries the aspirated sample to be transferred to the upper side of the 96-well plate 8, and then moves downward, and the pipetter 3 injects 200uL of the sample into the 96-well plate 8 by the action of the pump.
The pipette 3 is moved up again by the motor and moved laterally over the waste port 16 shown in fig. 4, thereby ejecting the tip 13.
This was repeated in sequence to transfer samples from different sample tubes to different wells of a 96-well plate, column 1 and column 4 containing lysate, respectively.
As shown in FIG. 1, the magnetic rod 4 (displacement magnetic rod 4) is inserted into the magnetic rod sleeve 14 shown in FIG. 3 and moved together downward into the wells containing magnetic beads in the 96- well plate arrays 3 and 6 shown in FIG. 5.
After standing for 30-60 seconds, the magnetic rod sleeve 14 with the magnetic beads adsorbed thereon is moved up and laterally to wells of the 96-well plate array 1 and array 4 containing lysate + sample, together with the magnetic rod 4.
Upwards draw away from bar magnet 4, keep bar magnet cover 14 motionless, lose the magnetic bead and release to the mixed liquid of lysate + sample. The motor drives the magnetic rod sleeve 14 to vibrate up and down slightly, so that the liquid in the hole is uniformly mixed. The sleeve 14 is then withdrawn from the bore.
After standing for 5 to 10 minutes, the magnetic rod 4 was inserted into the magnetic rod housing 14 and inserted into the wells of the 96- well plate arrays 1 and 4 together, and magnetic beads were adsorbed, and then moved up and moved laterally into the wells of the 96- well plate arrays 2 and 5 containing the washing solution 1 as shown in FIG. 5.
Upwards pull away from the magnetic rod 4, keep the magnetic rod sleeve 14 motionless, and the magnetic beads losing magnetic force are released into the washing solution 1. The motor drives the magnetic rod sleeve 14 to vibrate up and down slightly, so that the liquid in the hole is uniformly mixed. The sleeve 14 is then withdrawn from the bore.
The magnetic rod 4 is inserted into the magnetic rod cover 14 and is inserted into the wells of the 96- well plate arrays 2 and 5 together, adsorbs magnetic beads, and then moves up and moves laterally into the wells of the 96- well plate arrays 8 and 11 containing the washing solution 2 as shown in FIG. 5.
Upwards pull away from the magnetic rod 4, keep the magnetic rod sleeve 14 motionless, and the magnetic beads losing magnetic force are released into the washing liquid 2. The magnetic rod 4 is inserted again to adsorb magnetic beads, the magnetic rod 4 and the magnetic rod sleeve 14 integrally move upwards, and the magnetic rod sleeve 14 is suspended in the air for 1 minute after the lower end of the magnetic rod sleeve 14 completely leaves the 96-hole plate.
And then moved laterally into the wells of the 96 well plate columns 9 and 12 containing the eluent as shown in figure 5.
The magnetic rod 4 is upwards drawn away, the magnetic rod sleeve 14 is kept still, and the magnetic beads losing the magnetic force are released into the eluent. The motor drives the magnetic rod sleeve 14 to vibrate up and down slightly, so that the liquid in the hole is uniformly mixed.
The magnetic rod 4 is reinserted to adsorb the magnetic beads, and the magnetic rod 4 and the magnetic rod sleeve 14 move up integrally and then move laterally into the wells of the 96- well plate arrays 3 and 6. And finishing the extraction.
The pipette 3 shown in fig. 1 is moved by the motor to a position above the tip cassette B-15 shown in fig. 4, and then moved down to collect a tip. The pipette 3 and the tip 13 are moved to the position of row 9, row a shown in fig. 5 by the motor, and moved down to submerge the tip 13 below the liquid level in the hole, and the pipette 3 sucks 10uL of sample by the pump.
The pipetter 3 carries the aspirated sample to be transferred to the upper part of the eight-linked pipe frame 9 shown in fig. 4, then moves downwards, and injects the sample in the suction head into the detection liquid pre-filled in the eight-linked pipe frame 9 under the action of the pump.
The pipette 3 is moved up again by the motor and moved laterally over the waste port 16 shown in fig. 4, thereby ejecting the tip 13.
The operation was repeated in sequence until all the samples in the total rows 9 and 12 of the 96-well plate were transferred to the octal-rack 9.
The cap extractor 5 shown in fig. 1 is driven by a motor to move to the position above the eight-tube cap rack 10 shown in fig. 3, and then moves downwards to grab an eight-tube cap.
The cap extractor 5 drives the eight-connection pipe cap to transversely move to the position above the eight-connection pipe, and then the eight-connection pipe cap is pressed down under the driving of the motor to tightly cover the eight-connection pipe. Repeating the steps until all the eight-connected pipes are tightly covered.
The whole procedure is ended.
The improvement of the product mainly lies in that: 1. the motor-controlled clamp can stably clamp sample tubes with various shapes. 2. The rotating head capable of rotating forward and backward can realize the opening and closing of the sample tube. 3. The whole process of mutually matching the clamp, the rotating head, the pipettor, the magnetic rod sleeve and the cap extractor is automatic. 4. The bottoms of the 96-hole plate and the eight-connecting pipe can independently control a single-row temperature controller 23 for heating and cooling. 5. And the upper air inlet and the lower air outlet are arranged on the air filter. 6. The cover extractor can be compatible with the octuple pipe covers with different edge shapes.
The product can replace all manual operations in the prior art through a full-automatic instrument. Through the preset program, the operation is carried out by one key, and the requirement on the professional degree of an operator is greatly reduced. The whole operation time is shortened, and the pretreatment operation is finished within 30 minutes. The closed instrument is provided with an air filtering system and an ultraviolet sterilization system, so that the safety of pretreatment operation is greatly enhanced.
Example two:
based on the product of the first embodiment, the present embodiment may adopt more alternatives including:
(1) the clamp can be matched with a pressure sensor to clamp sample tubes with different overall dimensions.
(2) The rotating head can cooperate pressure sensor, realizes adapting to different sample tube cover elasticity degree.
(3) The suction head box can be in an integral box shape, can also be in a rack shape, and can also be in a laminated rack shape.
(4) The tip magazine may be divided into tips of different sizes, such as A, B, or may have only one size.
(5) The pipettor can be single or two different pipettors to cooperate different suction heads to realize the suction of liquid with different volumes.
(6) The well shape of a 96-well plate may be square or circular; the bottom can be circular or conical; the bottom of the magnetic rod sleeve can be round, conical or flat.
(7) The eight-way tube specification can be 0.1mL or 0.2 mL; the octal cap edge may be flat or corrugated.
(8) The bottom of the 96-well plate and the eight-way pipe can be provided with or without a temperature control module.
(9) The sample pipe frame, the 96-hole plate frame, the pipette head box and the eight-connection pipe frame can be fixed on the base of the instrument and can also be in a drawing type, and the sample pipe frame, the 96-hole plate frame, the pipette head box and the eight-connection pipe frame can be drawn out to facilitate loading.
(10) The sampling volume of the original sample or the nucleic acid sample can be freely set.
(11) The residence time of the magnetic rod and the magnetic rod sleeve in each nucleic acid operation step can also be freely set.
(12) The original samples can be 8, 16, 32, or multiples of 96 and 8, and the corresponding octuplers can also be in different numbers such as 1 row, 2 rows, etc.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The utility model provides a processing apparatus before nucleic acid testing, includes sample pipe, 96 orifice plates and eight ally oneself with the pipe of arranging in the processing apparatus in advance, characterized by still including installing in the processing apparatus:
the cover screwing device is arranged beside the sample tube and is used for clamping the sample tube and loosening or screwing the sample tube cover;
the pipettor is arranged above the inside of the treatment instrument and used for carrying and transferring the sample;
the magnetic rod displacement device is arranged beside the 96-pore plate and is used for adsorbing nucleic acid and magnetic beads of a sample to realize the extraction operation of the sample in the 96-pore plate;
and the eight-pipe receiving device is arranged in the processing instrument and below the processing instrument and is used for receiving the extracted sample to be detected.
2. The pre-processing instrument for nucleic acid detection according to claim 1, wherein the cap screwing device comprises a clamp and a rotating head, the clamp clamps the sample tube and drives the sample tube cap to rotate by the rotating head, so as to loosen or tighten the sample tube cap.
3. The pretreatment instrument for nucleic acid detection according to claim 2, wherein the clamp is moved in the X-axis direction and the Y-axis direction to switch between different tubes; the rotating head moves in the X-axis direction, the Y-axis direction and the Z-axis direction, and switching among different pipes is realized.
4. The pretreatment instrument for nucleic acid detection according to claim 1, further comprising a tip and a waste discharge port, wherein the pipette is moved in the X-axis direction, the Y-axis direction and the Z-axis direction so as to pick up different tips and suck a sample by the tip; the treater be equipped with the waste discharge mouth, the suction head after the use is abandoned through the waste discharge mouth.
5. The pretreatment instrument for nucleic acid detection according to claim 1, wherein the magnetic rod displacement device comprises a displacement magnetic rod and a magnetic rod sleeve, the displacement magnetic rod is inserted into the magnetic rod sleeve, the displacement magnetic rod adsorbs magnetic beads with samples by displacement, and the extraction operation comprises washing, air drying and elution.
6. The pretreatment instrument for nucleic acid detection according to claim 1, wherein the eight-manifold receiving means comprises an eight-manifold rack, a cap extractor and a slide rail, the extracted sample is transferred to the eight-manifold of the eight-manifold rack by a pipette, and the cap extractor tightly covers the eight-manifold; the eight-linked pipe frame is connected in a sliding mode along the sliding track.
7. The pretreatment instrument for nucleic acid detection according to claim 1, further comprising a temperature controller, wherein the temperature controller is provided in the treatment instrument.
8. The pretreatment instrument for nucleic acid detection according to claim 1, further comprising an air filtration system, wherein the treatment instrument is provided with the air filtration system therein.
9. The pretreatment instrument for nucleic acid detection according to claim 1, wherein said 96-well plate is divided in advance and comprises: column 1 and column 4 are lysates; column 2 and column 5 are washing solution 1; columns 3 and 6 are magnetic beads; column 8 and column 11 are washing solution 2; the eluent is shown in columns 9 and 12.
10. The pretreatment instrument for nucleic acid detection according to claim 8, wherein the air filtration system comprises an air intake fan, an air intake filter, an air exhaust fan and an air exhaust filter, the air intake fan and the air intake filter are disposed at an upper portion of the treatment instrument, and the air exhaust fan and the air exhaust filter are disposed at a lower portion of the treatment instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110355141.5A CN112920948A (en) | 2021-03-31 | 2021-03-31 | Nucleic acid detection pretreatment instrument |
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