CN202830011U - Automated nucleic acid extraction platform - Google Patents
Automated nucleic acid extraction platform Download PDFInfo
- Publication number
- CN202830011U CN202830011U CN2012204343717U CN201220434371U CN202830011U CN 202830011 U CN202830011 U CN 202830011U CN 2012204343717 U CN2012204343717 U CN 2012204343717U CN 201220434371 U CN201220434371 U CN 201220434371U CN 202830011 U CN202830011 U CN 202830011U
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- acid extraction
- platform
- reagent
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to field of nucleic acid extraction, particularly relates to a universal automated nucleic acid extraction platform. The platform comprises two functional devices, which are a liquid workstation and a nucleic acid extractor, wherein the liquid workstation is used for adding specimens and a reagent, the nucleic acid extractor is used for extracting nucleic acid and is controlled by software so as to achieve full automation of the nucleic acid extraction. 6 functional zones including a specimen zone, a consumable zone, a reagent zone, a transfer zone, a nucleic acid extraction zone, and a waste zone are provided, wherein an optimal nucleic acid extraction zone is provided with a nucleic acid extractor King Fisher Flex. Operations such as scanning the specimen, adding the reagent and the specimen into a transfer zone reaction pool, transferring the reaction pool to the nucleic acid extraction area to conduct nucleic extraction, preparing polymerase chain reaction (PCR) liquid, and adding nucleic acid extractive are controlled and accomplished by a software program. The automated nucleic acid extraction platform has the advantages of being high in degree of automation, convenient to operate, and capable of improving nucleic acid extraction efficiency. The software can conduct data interaction with a laboratory information management system (LIMS), and achieve laboratory management standardization.
Description
Technical field:
The utility model belongs to the nucleic acid extraction field, is specially a kind of general automation nucleic acid extraction platform, and this platform has widely to be used, and can be used for the fields such as medical treatment, health, epidemic prevention, scientific research, technical supervision.
Background technology:
Nucleic acid is the basic object of molecular biology research, and the nucleic acid extraction step is the most important, the most basic in a molecular diagnosis link.The method of extracting DNA or RNA from biological sample has a variety of, traditional organic solvent extraction method for example, and pellosil column adsorption method, magnetic bead partition method and the charge method etc. that can be fit to carry out with 96 orifice plate patterns high throughput automated operation.Wherein, the magnetic bead partition method is the present the most ripe a kind of method of most widely used, automatization condition, and the molecular diagnosis product of the research and development such as the company such as Roche, Chiron, Abbott, Gen-probe, Biomerieux is all the product based on the magnetic bead partition method.
Magnetic bead is a kind of novel functionalization solid phase carrier, its pan coating has active group, can with various bioactivators generation coupling, with characteristics such as the flowability of liquid and solid magnetic materials, can displacement under the effect of outside magnetic field and concentrate, after removing foreign field, vibration or suction can be dispersed in the liquid again a little, it is very efficient and convenient that thereby the separation of the solid phase of making, liquid phase becomes, and can obtain the very high targeting substance of purity by simple wash-out.The paramagnetic particle method nucleic acid extracting reagent generally is comprised of components such as lysate, magnetic bead, washing lotion, elutriants.Lysate is a kind of protein denaturant, can make animals and plants, microbial cell, viral capsid cracking, and make the protein denaturation of being combined with nucleic acid, nucleic acid is free to be discharged, and the magnetic ball can adsorb nucleic acid specifically, washs by washing lotion, remove the impurity such as protein, again because of the nucleic acid of elutriant Dissociative on magnetic bead, obtain all very high nucleic acid of purity and concentration, can be used for that pcr amplification, enzyme are cut, molecular hybridization etc.
The quality of the process of nucleic acid extraction and the nucleic acid that extracts affects greatly the result of molecular diagnosis, as can not effectively finishing the work of nucleic acid extraction aspect, just can not carry out the molecular diagnosis experiment.In the clinical diagnosis laboratory, the process of nucleic acid extraction is manual finishing substantially, because its working strength is large, usually brings the problems such as wrong-way, crossed contamination.Along with the fast development of molecular diagnosis, in clinical, build an automatic nucleic acid extraction platform and seem more and more important.On the one hand, it is rarely found that the automatic nucleic acid of domestic production extracts platform, and relevant patent documentation is more rare.The similar patent documentation that can find at present has " integrated blood nucleic acid screening platform " (Granted publication number: CN201695047U).On the other hand, automatic nucleic acid on the market extracts platform mainly by external producer, such as Tecan, Hamilton, PE, Beckman etc. provides, most of towards scientific research field, since its process sample capability volume little (being lower than 200 μ l/ samples), flux low (＜=48 samples/batch), with laboratory information management system (LIMS) can't interactive information etc. problem, substantially do not obtain the approval of clinical user, use few.The automatic equipment of other suitable clinical molecular diagnosis has the MagNA Pure of Roche company, the Procleix TIGRIS System of Chiron company etc.Yet the MagNA Pure of Roche is only applicable to the reagent of Roche, and the Procleix TIGRIS System of Chiron is only applicable to the reagent of Chiron and Gen-probe, is open platform not basically.
In sum, a kind of high-throughput is badly in need of in this area, general automatic nucleic acid extracts the automation issues that platform solves nucleic acid extraction.
Summary of the invention:
The utility model provides a kind of automatic nucleic acid and extracts platform.On this platform, can realize that paramagnetic particle method extracts the automatization leaching process of reagent, obtains the reliable nucleic acid product of steady quality.
Extract in the platform design at automatic nucleic acid of the present utility model, considered that emphatically the aerosol that prevents of nucleic acid extraction platform forms, prevents the problem of the aspects such as sample cross contamination, stability and automatization.Basis at the investigation many families in market equipment on sale is found: (1) liquid working station is the platform of a maturation, particularly the liquid working station application of sample that provides of the company such as Tecan, Hamilton accurate, stable, can match the move operation that mechanical manipulator is realized standard component, be very suitable for as the basis that makes up general nucleic acid extraction platform; (2) semi-automatic instrument for extracting nucleic acid has in clinical labororatory comparatively widely and to use, and its infective use shows, such product performance are stable, technology maturation, cheap, volume is small and exquisite, can be used as work alone unit and the coupling of liquid working station.
But, at home and abroad there is no liquid working station and the two complete combination of instrument for extracting nucleic acid, report brand-new, full automatic nucleic acid extraction workstation forms a concept.In some cases, although successfully the two is united use, but in the nucleic acid extraction process, also need the experimenter to participate in manual operations from liquid working station mobile working element to semi-automatic instrument for extracting nucleic acid, it or not the automated production on the complete meaning, moreover, do not consider in its design that anti-aerosol forms and anti-pollution design.
Comparatively speaking, maximum characteristics of the present utility model are liquid working station and semi-automatic instrument for extracting nucleic acid are integrated, the fast and accurately application of sample function at liquid working station and the efficient nucleic acid extraction function of semi-automatic instrument for extracting nucleic acid are combined, two kinds of equipment are integrated in a pedestal workspace, by software control, construct the nucleic acid extraction platform of a series of full-automatic operations such as sample application, reagent packing, nucleic acid extraction, the preparation of PCR reaction solution.This design has not only guaranteed the speed of nucleic acid extraction, prevents that aerosol from forming but also efficiently solve in blending manner and isolating nucleic acid design, and greatly degree has reduced the pollution of nucleic acid problem.
The utility model also has the characteristics such as its versatility, convenience, informationization in addition.Versatility is that the utility model belongs to open platform, and the paramagnetic particle method that does not repel any producer extracts reagent, can not only be used for blood screening, also can be used for the experiment of clinical hospitals molecular diagnosis, also can be used for the mechanisms such as Center for Disease Control, scientific research institutions; Convenience be the accessible sample size of the utility model from such as 20 μ l-2ml all can, a batch flux can be such as 24-96 sample, can continuous batch of sample introduction etc.; Informationization is that the control of all flow processs carries the bar code scanning recognition system, and by the whole flow processs of computer software control, and record files automatically, its result can with laboratory information management system (LIMS) interaction data, realize the laboratory information unified management.
Automatic nucleic acid of the present utility model extracts platform and comprises that pedestal work area and at least one are used for the mechanical manipulator of realizing fluid operated liquid sample arm and/or being used for realizing the move operation of standard component.Wherein said pedestal work area is divided into some functional zone, and described functional zone include but not limited to sample area, consumptive material district, reagent area, transfer district, nucleic acid extraction district and waste district.Particularly:
(1) sample area is used for placing experiment sample to be checked, and described sample to be checked is arranged in the sample pool of sample area, and described sample pool includes but not limited to heparin tube, standard 24 orifice plates, standard 48 orifice plates, standard 96 orifice plates etc.Preferably, wherein each sample pool respectively carries barcode, can be by the autoscan recognition and verification.
(2) the consumptive material district is used for placing the required consumptive material of experiment, and described consumptive material includes but not limited to suction nozzle carrier, deep-well plates and PCR plate; The suction nozzle carrier is for placing special-purpose suction nozzle, and deep-well plates is used for nucleic acid extraction, and the PCR plate is used for the preparation of amplification reaction solution; Preferably, each consumptive material respectively carries barcode, can be by the autoscan recognition and verification.
(3) reagent area is used for placing the required reagent of experiment, and described reagent is arranged on the reagent carrier of reagent area; Preferably, each reagent respectively carries barcode, can be identified by autoscan.
(4) the transfer district is provided with carrier and includes but not limited to that for placing the elements such as deep-well plates, PCR plate are used for the operations such as sample pipetting volume, reagent interpolation, the configuration of PCR reaction solution; These elements can by mechanical manipulator in the transfer district, the zone such as reagent area and nucleic acid extraction district shifts.
(5) the nucleic acid extraction district is used for placing the required nucleic acid-extracting apparatus of realization nucleic acid extraction.This district is critical area of the present utility model, and placing semi-automatic instrument for extracting nucleic acid in this zone is unique design of the present utility model, the blending manner that semi-automatic instrument for extracting nucleic acid has adopted bar magnet to stir up and down.The equipment that the leading firm of present this area produces (for example adopts vortex vibration mixing more, the equipment that is used for Individual Identification System that Tecan company provides adopts this mode more) and (for example repeatedly blow and beat mixing, the equipment that is used for R﹠D institution that Hamilton provides adopts this mode more) etc. blending manner, such blending manner easily produces aerosol, cause pollution of nucleic acid, the reagent of different manufacturers is worked at this type of platform, and to pollute probability higher, is about 0.5%-10%.Compare with above-mentioned other blending manner, the blending manner that described bar magnet stirs up and down has aerosol and forms the advantages such as probability is low, crossed contamination is little.In addition, be different from the way that waste liquid is abandoned in conventional suction, the semi-automatic instrument for extracting nucleic acid that the utility model extracts the district will extract that waste liquid is static to be placed in the former consumptive material, realize separate nucleic acid by separating the mode that shifts magnetic bead, therefore, inhale the mode of abandoning liquid than tradition, the utlity model has aerosol and form the advantage that probability is low, the experimental situation pollution is little.In an embodiment of the present utility model, the semi-automatic instrument for extracting nucleic acid in described nucleic acid extraction district can be selected but be not limited to KingFisher Flex magnetic bead separation and purification system, MagMAXExpress nucleic acid extraction system, is preferably KingFisher Flex magnetic bead separation and purification system.
(6) the waste district is used for placing the waste that experiment produces, for example solid waste and/or liquid debris; Preferably, can comprise discarded suction nozzle gathering barrel, waste liquid tank etc. in the described waste district.
The above-mentioned functions district can adjust according to applicable cases, and the number of elements of each workspace can increase and decrease according to applicable cases.Preferably described each functional zone are positioned on the plane that the X-axis of described pedestal work area and Y-axis form, according to shown in the drawings arranged sequentially.So both that each workspace is relatively independent separately, avoid the mutual pollution in the zones such as sample area, nucleic acid extraction district, reagent area, maximizing is saved the working basic stand space again.Except above-mentioned 6 functional zone, also can increase other functional areas, be used for deep-well plates and/or PCR plate are carried out the sealer processing such as the sealer district.
(being the top, plane of described pedestal work area) is liquid sample arm and/or mechanical manipulator on Z-direction, on the wherein said liquid sample arm with liquid-transfering device (for example sample injector), can carry out pipetting to the element on the described pedestal work area, it can move at X, Y, Z axis; Described mechanical manipulator can carry out move operation to the element on the described pedestal work area (for example deep-well plates), can move at X, Y, Z axis.
In the use procedure of nucleic acid extraction platform of the present utility model, finished such as the following step by software control: the autoscan of sample and counting, reaction tank is placed the transfer district, reagent and/or sample are added reaction tank, reaction tank is transferred to the nucleic acid extraction district carry out nucleic acid extraction etc.The nucleic acid extraction process automation degree of nucleic acid extraction platform of the present utility model is high, easy to operate, preferably, described nucleic acid extraction platform operations software database system, can with laboratory information management system (LIMS) interaction data, realize the laboratory room managing stdn.
In addition, utilizing the accessible sample of nucleic acid extraction platform of the present utility model can be any material that comprises nucleic acid molecule, includes but not limited to sputum, urine, serum, blood plasma, tissue homogenate thing, cerebrospinal fluid, culture etc.Can be used for PCR, RT-PCR, TMA, NASBA, 3SR, LCR, endonuclease reaction, ligation, conversion, the experiment of transfection equimolecular via the product after the nucleic acid extraction platform processes of the present utility model.In addition, because nucleic acid extraction platform of the present utility model can be realized automatization, the informationization of testing having reduced reviewer's working strength, greatly reduced reviewer's mishandle, and be convenient to the unified management of data.
Below with reference to embodiment and accompanying drawing, further explain nucleic acid extraction platform of the present utility model.Those skilled in the art will understand that, following content only is intended to describe better spirit of the present utility model, thought, and can not become restriction of the present utility model.In addition, according to the description of this specification sheets, those skilled in the art will easily understand, and many versions of the present utility model all can not exceed purport spirit of the present utility model, and fall in the protection domain of the present utility model.
Description of drawings
Fig. 1: automatic nucleic acid extracts the platform plane schematic diagram
Number in the figure
1 sample area
2 consumptive material districts
3 reagent areas
4 transfer districts
5 nucleic acid extraction districts
6 waste districts
7KingFisher Flex magnetic bead separation and purification system
8 reagent store the low temperature module
1. specimen holder
2. suction nozzle is discarded the storeroom
3. liquid is discarded the storeroom
4. 96 hole depth orifice plates
5. 96 hole depth orifice plates and 96 magnetic head sleeve pipes
6. PCR plate
7. reagent trough
8. magnetic bead storage rack
9. 1000 μ l suction nozzles
10. 200 μ l suction nozzles
Embodiment is described in further details the present invention below by example:
Embodiment 1:
Select Tecan FREEDOM EVO-2100/150/200Bace liquid working station and KingFisher Flex magnetic bead separation and purification system to make up, 6 functional zone such as sample area, consumptive material district, reagent area, transfer district, nucleic acid extraction district, waste district are set.
(1) sample area be used for to be placed experiment sample to be checked, and sample pool is heparin tube or standard 24 orifice plates, standard 48 orifice plates, standard 96 orifice plates etc.Each sample pool respectively carries barcode, can be by the autoscan recognition and verification.
(2) the consumptive material district is used for placing the required consumptive material of experiment, and described consumptive material comprises suction nozzle carrier, deep-well plates and PCR plate; The suction nozzle carrier is for placing special-purpose suction nozzle, and 96 deep-well plates are used for the preparation of amplification reaction solution for nucleic acid extraction, PCR plate; Each consumptive material respectively carries barcode, can be by the autoscan recognition and verification.
(3) reagent area is used for placing the required reagent of experiment, and such as paramagnetic particle method nucleic acid extracting reagent, PCR reaction solution etc., described reagent is arranged on the reagent carrier of reagent area; Each reagent respectively carries barcode, can be identified by autoscan.
(4) the transfer district is provided with carrier and comprises that for placing the elements such as deep-well plates, PCR plate are used for the operations such as sample pipetting volume, reagent interpolation, the configuration of PCR reaction solution; These elements can by mechanical manipulator in the transfer district, the zone such as reagent area and nucleic acid extraction district shifts.
(5) the nucleic acid extraction district arranges KingFisher Flex magnetic bead separation and purification system and carries out nucleic acid extraction.
(6) can comprise discarded suction nozzle gathering barrel, waste liquid tank in the waste district.
Techniqueflow:
The user puts into sample on the support of sample area, and sample area once can hold 1 ~ 576 duplicate samples (16 * 36=576 parts).The user clicks " scanning " button at the software operation interface, the Tecan scanning element begins the sample bar code is scanned, sampling amount position 120 μ l), choose the operation such as test item the schematic diagram that software interface occurred and the sample discharging is corresponding after scanning was finished, the user can click that schematic diagram is checked the sample bar code information, sample is divided into groups, selects the sample mix mode, and (single inspection: sampling amount position 960 μ l, 6 mix: sampling amount position 160 μ l, 8 mix:.
In programming interface selection operation program, schedule of operation can be selected " mixed sample+extraction+PCR dosing+reagent template packing ", certain operation in the middle of also can reducing according to user's needs, by manual replacement, increased the handiness of experiment, program can arrange 1 ~ 5 and take turns, and can realize 576 1 ~ 5 times sample size.Choose complete software according to before the user arrange and calculate this test and sweep the reagent of needs, consumable quantity and prompting user and load the reagent consumptive material in the corresponding position: place suction nozzle at the suction nozzle carrier in consumptive material district, be provided with carrier in the transfer district and comprise the elements such as deep-well plates, PCR plate for placing.Place experiment required LH buffer, washbuffer, W/Ebuffer, magnetic bead, PCR reaction solution etc. at reagent area.But reagent consumptive material loaded user's manual confirmation or confirm by instrument scanning.After clicking operation, instrument begins to carry out the sample married operation, draw a certain amount of sample (the different sample volumes according to mixed sample loading mode are different) at sample area and join in the 96 hole depth orifice plates in transfer district, the sample married operation can be finished at 0 ~ 1.5 hour according to user's set-up mode.
Sample mix is complete, sample injector from reagent area draw LH buffer, the 500 μ l of 960 μ l Washbuffer, 180 μ l W/Ebuffer, 180 μ l magnetic beads, join 5 96 hole depth orifice plates in transfer district respectively.
Mechanical arm gripping transfer district has been added with sample and has extracted 5 96 hole depth orifice plates of reagent and move to 1 ~ 6 of KingFisher Flex magnetic bead separation and purification system that the nucleic acid extraction district arranges with 1 96 orifice plate of magnetic head sleeve pipe and carry out nucleic acid extraction, is extracted in 2 hours and finishes.Leaching process relates to reactions such as carrying out heating pyrolyze, cooling, magnetic bead absorption nucleic acid in the 1st, 2 96 orifice plates, absorption has the magnetic bead of nucleic acid to remove albumen, salt etc. through the cleaning of the Washbuffer in the 3rd block of plate, the 4th W/E buffer, wash-out in the W/E buffer of the 5th 96 orifice plates, discharge nucleic acid, nucleic acid just is extracted purifying out like this.
In nucleic acid extraction, logical mechanical manipulator gripping PCR reaction reagent and PCR plate move to the transfer district, and sample injector is prepared the PCR reaction solution and is sub-packed in the PCR plate in the transfer district.Treat nucleic acid extraction district end of operation, the 5th 96 orifice plates that nucleic acid extractive is equipped with in the mechanical manipulator gripping are positioned over the transfer district, sample injector divides nucleic acid in the PCR plate that installs to the PCR reaction solution, and end of operation software diagram reminding user will divide the PCR plate sealer that installs or add a cover to be put in the PCR instrument and detect.Prompting user is removed the rubbish that reaches the waste district on the instrument table top simultaneously.
PCR instrument detected result can import in the software of instrument, and software generates the detected result of sample automatically.
Embodiment 2:
Adopt this full-automatic nucleic acid extraction platform to extract Chinese pharmaceutical biological product and examine and determine graduate HBV nucleic acid reference material, HIV nucleic acid reference material, detect at quantitative real time PCR Instrument, test item and detected result are as follows:
HBV nucleic acid reference material comprises: positive reference material P1-P9, negative reference material N1-N8, sensitivity reference material L0 with negative diluted plasma to 10IU/ml.
HIV-1 nucleic acid reference material comprises: positive reference material P1-P8, negative reference material N1-N8, the sensitivity reference material with negative diluted plasma to 50IU/ml.
The positive reference material P1-P9 of detected result: HBV, coincidence rate 9/9; Negative reference material N1-N10 coincidence rate 10/10.The specificity that this platform HBV DNA extraction is described is good.
Detected result: detect the positive reference material P1-P8 of HIV-1, negative reference material N1-N10 coincidence rate 8/8.The specificity that this platform HIV-1RNA extraction is described is good.
With HBV sensitivity reference material with negative diluted plasma to 10IU/ml, duplicate detection 20 times, recall rate 〉=95% illustrates that this platform is good to the extraction sensitivity of HBV DNA.
With HIV sensitivity reference material with negative diluted plasma to 50IU/ml, duplicate detection 20 times, recall rate 〉=95% illustrates that this platform is good to the extraction sensitivity of HIV-1RNA.
By this example explanation, this platform all has preferably extraction efficiency to DNA and RNA, and specificity is good.Than the similar patent " integrated blood nucleic acid screening platform " that can find at present (Granted publication number: extraction efficiency CN201695047U) superior (HBV DNA sensitivity is 20IU/ml during similar patent recall rate 〉=95%, and HIV-1RNA sensitivity is 100IU/ml)
According to disclosure of the present utility model, the barren great many of experiments of those skilled in the art can be able to successful implementation to nucleic acid extraction platform of the present utility model.The disclosed example of the utility model only is that the utility model is described, but does not consist of restriction of the present utility model.Those skilled in the art are with similar sub (such as similar liquid working station or similar instrument for extracting nucleic acid) or transform; or the content of describing with alternative this example of relevant chemistry and biology preparation changes; but do not exceed spirit of the present utility model, scope and thought, all fall into the claimed scope of the utility model.
Claims (4)
1. a general automatic nucleic acid extracts platform, it is characterized in that, make up with liquid working station and instrument for extracting nucleic acid, the pedestal work area of this nucleic acid extraction platform arranges following six functional zone:
Sample area: be used for placing experiment sample to be checked;
Consumptive material district: be used for placing the required consumptive material of experiment;
Reagent area: be used for placing the required reagent of experiment;
Transfer district: be provided with carrier, include but not limited to the elements such as deep-well plates, PCR plate for placing, be used for the operations such as sample pipetting volume, reagent interpolation, the configuration of PCR reaction solution;
Nucleic acid extraction district: be used for placing the required instrument for extracting nucleic acid of realization nucleic acid extraction;
Waste district: be used for placing the waste that experiment produces.
2. automatic nucleic acid claimed in claim 1 extracts platform, and wherein said liquid working station is Tecan FREEDOM EVO-2100/150/200Bace series liquid working station or HAMILTON MICROLAB STAR series liquor removing workstation.
3. claim 1 or 2 described automatic nucleic acids extract platform, and wherein said instrument for extracting nucleic acid is KingFisher Flex magnetic bead separation and purification system or MagMAX Express nucleic acid extraction system magnetic bead separation and purification system.
4. claim 1 or 2 described automatic nucleic acids extract platform, and wherein there are liquid sample arm and mechanical manipulator in top, pedestal work area, and the liquid sample arm realizes that liquid adds operation, and mechanical manipulator is realized standard original paper move operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012204343717U CN202830011U (en) | 2012-08-29 | 2012-08-29 | Automated nucleic acid extraction platform |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012204343717U CN202830011U (en) | 2012-08-29 | 2012-08-29 | Automated nucleic acid extraction platform |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202830011U true CN202830011U (en) | 2013-03-27 |
Family
ID=47941766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012204343717U Expired - Lifetime CN202830011U (en) | 2012-08-29 | 2012-08-29 | Automated nucleic acid extraction platform |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202830011U (en) |
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN104975090A (en) * | 2015-07-09 | 2015-10-14 | 广州安能特化学科技有限公司 | Method for automatically detecting abundance of butane oxidizing bacteria |
| CN104988052A (en) * | 2015-06-25 | 2015-10-21 | 中山鼎晟生物科技有限公司 | Quick virus detection kit and using method thereof |
| CN105018609A (en) * | 2015-07-09 | 2015-11-04 | 广州安能特化学科技有限公司 | Automatic detection method of abundance of methane-oxidizing bacteria |
| WO2015192330A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extraction device and method |
| CN105296468A (en) * | 2015-10-19 | 2016-02-03 | 常熟市公安局 | Method of DNA extraction |
| CN105647797A (en) * | 2014-12-03 | 2016-06-08 | 深圳华大基因研究院 | Nucleic acid processing device based on paramagnetic particle method, nucleic acid processing equipment, and kit |
| CN106119382A (en) * | 2016-07-19 | 2016-11-16 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection method and application thereof |
| CN106190816A (en) * | 2016-07-19 | 2016-12-07 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection equipment |
| WO2017024899A1 (en) * | 2015-08-11 | 2017-02-16 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation |
| CN106520540A (en) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | Integrated full-automatic nucleic acid detection reaction system |
| CN106929406A (en) * | 2017-05-23 | 2017-07-07 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the compact device for detecting |
| CN107345199A (en) * | 2016-05-05 | 2017-11-14 | 王磊 | Biological sample preparation system |
| CN108504542A (en) * | 2018-05-24 | 2018-09-07 | 南京岚煜生物科技有限公司 | Full-automatic nucleic acid extraction amplification diagnosis all-in-one machine |
| CN108588066A (en) * | 2018-05-08 | 2018-09-28 | 广州高盛智造科技有限公司 | A kind of equipment and its control method automatically extracting nucleic acid |
| CN108795688A (en) * | 2018-05-24 | 2018-11-13 | 南京岚煜生物科技有限公司 | The nucleic acid extraction module of diagnosis all-in-one machine is expanded for full-automatic nucleic acid extraction |
| CN108865659A (en) * | 2018-09-19 | 2018-11-23 | 基蛋生物科技股份有限公司 | Nucleic acid extraction and amplification fluorescent detection system |
| CN108949548A (en) * | 2018-09-14 | 2018-12-07 | 昆明医科大学第附属医院 | A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions |
| CN110157702A (en) * | 2019-05-24 | 2019-08-23 | 中国水产科学研究院南海水产研究所深圳试验基地 | It is a kind of for extracting the acquisition station of coral RNA sample |
| CN111443172A (en) * | 2020-04-17 | 2020-07-24 | 上海纽钛测控技术有限公司 | Distributed food detection method |
| CN112210476A (en) * | 2020-11-12 | 2021-01-12 | 药明激创(佛山)生物科技有限公司 | Be applied to biological liquid workstation |
| CN112920948A (en) * | 2021-03-31 | 2021-06-08 | 苏州博捷特生物科技有限公司 | Nucleic acid detection pretreatment instrument |
| CN113219189A (en) * | 2021-05-06 | 2021-08-06 | 复旦大学附属肿瘤医院 | Sample automated processing system |
| CN113308364A (en) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | Nucleic acid extraction substrate, nucleic acid extraction apparatus, and nucleic acid extraction method |
| CN114540342A (en) * | 2022-01-18 | 2022-05-27 | 上海析维医疗科技有限公司 | Nucleic acid extraction method and system |
| CN114736767A (en) * | 2022-04-08 | 2022-07-12 | 吉林大学 | Nucleic acid extraction system |
-
2012
- 2012-08-29 CN CN2012204343717U patent/CN202830011U/en not_active Expired - Lifetime
Cited By (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| WO2015192330A1 (en) * | 2014-06-17 | 2015-12-23 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extraction device and method |
| CN105940105A (en) * | 2014-06-17 | 2016-09-14 | 深圳迈瑞生物医疗电子股份有限公司 | Nucleic acid extraction device and method |
| CN105647797A (en) * | 2014-12-03 | 2016-06-08 | 深圳华大基因研究院 | Nucleic acid processing device based on paramagnetic particle method, nucleic acid processing equipment, and kit |
| CN105647797B (en) * | 2014-12-03 | 2018-01-12 | 深圳华大基因研究院 | Nucleic acid processing unit and nucleic acid processing equipment and kit based on paramagnetic particle method |
| CN104988052A (en) * | 2015-06-25 | 2015-10-21 | 中山鼎晟生物科技有限公司 | Quick virus detection kit and using method thereof |
| CN105018609B (en) * | 2015-07-09 | 2019-02-01 | 广州安能特化学科技有限公司 | A kind of automated detection method of methane-oxidizing bacteria abundance |
| CN105018609A (en) * | 2015-07-09 | 2015-11-04 | 广州安能特化学科技有限公司 | Automatic detection method of abundance of methane-oxidizing bacteria |
| CN104975090B (en) * | 2015-07-09 | 2018-07-20 | 广州安能特化学科技有限公司 | A kind of automated detection method of butane oxidation bacterium abundance |
| CN104975090A (en) * | 2015-07-09 | 2015-10-14 | 广州安能特化学科技有限公司 | Method for automatically detecting abundance of butane oxidizing bacteria |
| WO2017024899A1 (en) * | 2015-08-11 | 2017-02-16 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation |
| CN105296468A (en) * | 2015-10-19 | 2016-02-03 | 常熟市公安局 | Method of DNA extraction |
| CN107345199A (en) * | 2016-05-05 | 2017-11-14 | 王磊 | Biological sample preparation system |
| CN106190816B (en) * | 2016-07-19 | 2018-07-20 | 湖州高亿诺生物科技有限公司 | Automate microbial molecules detection device |
| CN106119382A (en) * | 2016-07-19 | 2016-11-16 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection method and application thereof |
| WO2018014678A1 (en) * | 2016-07-19 | 2018-01-25 | 湖州高亿诺生物科技有限公司 | Automated microbial molecular assay device and method |
| CN106190816A (en) * | 2016-07-19 | 2016-12-07 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection equipment |
| CN106119382B (en) * | 2016-07-19 | 2018-08-28 | 湖州高亿诺生物科技有限公司 | Automate microbial molecules detection method and its application |
| CN106520540A (en) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | Integrated full-automatic nucleic acid detection reaction system |
| CN106929406A (en) * | 2017-05-23 | 2017-07-07 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the compact device for detecting |
| CN106929406B (en) * | 2017-05-23 | 2024-02-06 | 至农科技发展(浙江)有限公司 | Compact device for nucleic acid extraction and detection |
| CN108588066A (en) * | 2018-05-08 | 2018-09-28 | 广州高盛智造科技有限公司 | A kind of equipment and its control method automatically extracting nucleic acid |
| CN108588066B (en) * | 2018-05-08 | 2024-06-18 | 广州高盛智造科技有限公司 | Automatic nucleic acid extraction equipment and control method thereof |
| CN108504542A (en) * | 2018-05-24 | 2018-09-07 | 南京岚煜生物科技有限公司 | Full-automatic nucleic acid extraction amplification diagnosis all-in-one machine |
| CN108795688A (en) * | 2018-05-24 | 2018-11-13 | 南京岚煜生物科技有限公司 | The nucleic acid extraction module of diagnosis all-in-one machine is expanded for full-automatic nucleic acid extraction |
| CN108949548A (en) * | 2018-09-14 | 2018-12-07 | 昆明医科大学第附属医院 | A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions |
| CN108865659A (en) * | 2018-09-19 | 2018-11-23 | 基蛋生物科技股份有限公司 | Nucleic acid extraction and amplification fluorescent detection system |
| CN110157702A (en) * | 2019-05-24 | 2019-08-23 | 中国水产科学研究院南海水产研究所深圳试验基地 | It is a kind of for extracting the acquisition station of coral RNA sample |
| CN111443172A (en) * | 2020-04-17 | 2020-07-24 | 上海纽钛测控技术有限公司 | Distributed food detection method |
| CN112210476A (en) * | 2020-11-12 | 2021-01-12 | 药明激创(佛山)生物科技有限公司 | Be applied to biological liquid workstation |
| CN112920948A (en) * | 2021-03-31 | 2021-06-08 | 苏州博捷特生物科技有限公司 | Nucleic acid detection pretreatment instrument |
| CN113219189A (en) * | 2021-05-06 | 2021-08-06 | 复旦大学附属肿瘤医院 | Sample automated processing system |
| CN113308364A (en) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | Nucleic acid extraction substrate, nucleic acid extraction apparatus, and nucleic acid extraction method |
| CN114540342A (en) * | 2022-01-18 | 2022-05-27 | 上海析维医疗科技有限公司 | Nucleic acid extraction method and system |
| CN114736767A (en) * | 2022-04-08 | 2022-07-12 | 吉林大学 | Nucleic acid extraction system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202830011U (en) | Automated nucleic acid extraction platform | |
| JP5089384B2 (en) | Apparatus and methods for separating, mixing and concentrating magnetic particles from fluids and their use in purification methods | |
| JP5886787B2 (en) | Equipment for processing samples | |
| US10458998B2 (en) | Device for processing samples | |
| CN102492603B (en) | Automated instrument for nucleic acid extraction and mass spectrum sample application | |
| US8597878B2 (en) | Device and procedure for automated isolation and purification of nucleic acids from complex starting materials of the users choice | |
| CN108588066B (en) | Automatic nucleic acid extraction equipment and control method thereof | |
| CA2920667C (en) | Automated system and method for culturing stem cells | |
| KR20180048558A (en) | Components, devices and methods of devices for purifying and testing biomolecules from biological samples | |
| JP4081468B2 (en) | Nucleic acid recovery instrument and method | |
| US9932574B2 (en) | Suspension container for binding particles for the isolation of biological material | |
| CN105164509A (en) | Method and kit for purifying nucleic acids | |
| JP3220438U (en) | Sample extraction device | |
| JP6894453B2 (en) | Sample preparation cartridge and how to use it | |
| CN1511191A (en) | Apparatus for purifying nucleic acid and method of purifying nucleic acid | |
| WO2020001493A1 (en) | Concentrated and enriched magnetic bead purification instrument | |
| RU110746U1 (en) | DEVICE FOR SIMULTANEOUS AUTOMATED ISOLATION AND PURIFICATION OF NUCLEIC ACIDS FROM SEVERAL BIOLOGICAL SAMPLES | |
| CN112210476A (en) | Be applied to biological liquid workstation | |
| CN109182335A (en) | A kind of Viral nucleic acid extraction reagent box | |
| Akutsu et al. | Development of an integrated automation system with a magnetic bead‐mediated nucleic acid purification device for genetic analysis and gene manipulation | |
| CN212504917U (en) | Full-automatic nucleic acid extraction appearance | |
| EP2423688A1 (en) | Suspension container for binding particles for the isolation of biological material | |
| CN110408610A (en) | Nucleic acid extracting reagent item | |
| CN108642050A (en) | PCR purified reagents and the method for using the reagent purification PCR product | |
| EP1437407B1 (en) | Apparatus for collecting nucleic acid and method of collecting nucleic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130327 |
|
| CX01 | Expiry of patent term |