CN108949548A - A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions - Google Patents
A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions Download PDFInfo
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- 238000011901 isothermal amplification Methods 0.000 title claims abstract description 18
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The present invention discloses a kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, is related to technical field of medical examination.The full-automatic nucleic acids instrument, including nucleic acid-extracting apparatus, detection device and controller, nucleic acid-extracting apparatus are connected with detection device and are equipped with sealing cover, and detection device side installation controller simultaneously connects to power supply.Full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions of the invention, can realize the continuous detection of sample of nucleic acid, the influence of not examined batch, and detection process whole-course automation can reduce technical threshold, be conducive to this technology and promote to base.
Description
Technical field
The present invention relates to technical field of medical examination, are specifically related to a kind of full-automatic nucleic acid based on isothermal amplification reactions
Amplification Analysis instrument.
Background technique
Nucleic acid (Ribonucleic RNA, DNA DNA) is the basic unit for representing life entity hereditary feature, nucleic acid
Detection is to carry out advanced biological detection on a molecular scale, compared to traditional morphologic detection, cytology detection, immunology inspection
The remarkable advantages such as survey etc. more sensitivity, specificity are widely used in hepatitis, AIDS in the detection of clinical position amplifying nucleic acid in recent years
Etc. serious infectious diseases；The emerging infectious diseases such as SARS, bird flu, hand-foot-and-mouth disease；The gene diagnosis and polygenes phase of genetic disease
The chronic disease detection and diagnosis of pass.Detection of nucleic acids includes qualitative PCR (Polymerase Chain Reaction, polymerase chain
Reaction), real-time fluorescence quantitative PCR (Real time PCR, QPCR), molecule hybridization, the technologies such as genetic chip and gene sequencing,
Gene qualitative and quantitative analysis can be achieved.The detections such as molecule hybridization, genetic chip and gene sequencing, and the premise of these technologies is
It realizes the amplification of target gene, only just can be carried out corresponding detection after the amplification of realization target gene.
In addition, detection of nucleic acids need to have efficient and high-purity method for extracting nucleic acid.Existing nucleic acid extraction is substantially adopted
With paramagnetic particle method, improvement is carried out with after surface modification with surface of the nanotechnology to superparamagnetic nano particle, is prepared into super suitable
Magnetic silicon oxide nanometer magnetic bead.This magnetic bead can specifically be identified with nucleic acid molecules on micro interface and efficiently be combined.Benefit
With the superparamagnetism of silica nanosphere, in the work of Chaotropic salt (guanidine hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field
Under, can from blood, animal tissue, food, pathogenic microorganism equal samples DNA and RNA separate, can be applicable to clinic
Medical diagnosis on disease, transfusion safety, Forensic Identification, nucleic acid samples build library, environmental microorganism detection, food safety detection, molecule life
The multiple fields such as object research.
The traditional PCR technique that three temperature constantly recycle is depended in clinical and research work at present, this technology is only
Can carry out in batches, be difficult to add other detection samples when amplified reaction operation, at the same the method for nucleic acid extraction also because
The influence of round pcr is all into batch and extracts, therefore art methods have following defects that
1. traditional PCR method based on three temperature changes when detection can not continuous sample introduction, to detection sample
Batch is restricted, can not achieve the continuous detection of automation, does not meet the demand of the detection of clinical samples.
2. traditional PCR method is higher to temperature control requirement, and need to realize the accurate elevating control of temperature, therefore to wanting
Equipment is asked to have degree of precision, therefore equipment is relatively expensive, is unfavorable for Technique Popularizing.
3. temperature change has an impact to the performance of enzyme in normal PCR detection method, with the extension of reaction time the work of enzyme
Property constantly decline, influence reaction sensibility and specificity.
4. carrying out the biological sample of detection of nucleic acids, there are diversity, at the same because testing goal it is different required for target not yet
Together, some reaction templates need DNA and some then need RNA, and different samples and the method for extracting DNA or RNA are all different,
And the method that nucleic acid extraction product currently on the market is all made of batch extracting, i.e., multiple same type samples are extracted every time, or only
DNA or RNA can be extracted respectively, therefore restricted to the speed of entire detection reaction.
5. it is more that traditional PCR technique is related to link, inspection result quality just can guarantee after needing professional trained, because
This technical threshold is higher, is unfavorable for the popularization of technology.
Summary of the invention
In view of the above-mentioned problems existing in the prior art, the present invention provides a kind of full-automatic core based on isothermal amplification reactions
Sour Amplification Analysis instrument, can realize the continuous detection of sample of nucleic acid, the influence of not examined batch, and detection process is whole-process automatic
Change, technical threshold can be reduced, is conducive to this technology and is promoted to base.
To realize above-mentioned technical purpose and the technique effect, the present invention is achieved through the following technical solutions:
A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, including nucleic acid-extracting apparatus, detection device
And controller, nucleic acid-extracting apparatus are connected with detection device and are respectively equipped with gas-tight silo, detection device and extraction element respectively with
Controller simultaneously connects to power supply；
The nucleic acid-extracting apparatus includes that sample sample introduction track, reagent track, nucleic acid extraction unit and sample go out sample track,
The injection port of the sample sample introduction track connects specimen holder propeller, and rear side installs sample blending device, and connects reagent track,
Reagent track two sides separately design the reagent suction needle and sample suction needle controlled by mechanical arm, and are correspondingly arranged extraction reagent
Storehouse, TIP storehouses and waste holder, reagent track end connect each nucleic acid extraction unit by branching agent track, and the nucleic acid mentions
Taking is reagent track among unit, and track two sides are nucleic acid extraction position, the magnetic of the upper layer installation specification arrangement of nucleic acid extraction unit
Stick is simultaneously controlled by bar magnet moving arm, and nucleic acid extraction unit rear end connection sample goes out sample track, and sample track end is after extracting out
Mark product storage area；
The detection device includes reaction tray and ccd image sensor (CCD) device, in the reaction tray
Equipped with multiple reaction cups, and design sample outlet and detection reagent sample introduction rail, sample exhaust port and detection reagent sample introduction track
Mechanical gripper is designed with reaction tray connecting pin, sample exhaust port is connected sample waste storage storehouse, pacified on detection reagent sample introduction track
Sample bottle capper is filled, liquid injection port and temperature detection mouth are designed in reaction tray, CCD device is used for fluorescence detection.
Further, the nucleic acid-extracting apparatus upper end connects TIP loading channels and reagent storage storehouse, the nucleic acid mention
Take unit and detection unit that ultraviolet lamp is installed.
Further, TIP storehouses in the detection device, agent bin and the sample suction needle controlled by mechanical arm and
Propeller；
Further, exhaust equipment is equipped on rear side of nucleic acid-extracting apparatus and nucleic acid detection apparatus.
The reagent position in storehouse refrigeration warehouse.
A kind of full-automatic nucleic acid amplification assays instrument control system based on isothermal amplification reactions, including controller, the control
Device processed includes detection unit, control unit, execution unit and display unit, and detection unit includes the bar code reading of sample introduction track
Device, reaction tray temperature measuring probe, control unit and display unit are main control chip ((the AT89C51 monolithic for being mounted on controller
Machine core piece)) and display, execution unit include the control mechanical arm of reagent needle and specimen needle, sample sample introduction track, reagent rail
Road, sample go out sample track, the handgrip of bar magnet moving arm and detection device, mechanical arm；Barcode reader detection signal feeds back to control
Sample is sent to nucleic acid extraction unit, and drive control by the sample sample introduction track of unit processed, control unit driving executive component
Mechanical arm, reagent track and bar magnet moving arm carry out nucleic acid extraction, after the completion of extracting driving sample go out sample track by mark product send to
Storage area drives handgrip, the mechanical arm of detection device to be detected later.
Controller control specimen needle, reagent needle and bar magnet do not conflict, that is, can also be other extractions in nucleic acid extraction
Position reagent adding and sample again.
A method of the nucleic acid extraction and detection of the full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, including
Following steps:
Specimen holder to be checked send to injection port to wait and be loaded through sample sample introduction track, and through barcode reader identification sample letter
Breath, the sample if necessary to mixing are then loaded after rotation mixes in mark product evenly mixing device again, go out sample through sample after sample-adding completion
Sample storage area after track is sent to detection；
It extracts reagent and pushes to reagent track from agent bin, and add other additional now with reagent；
Sample needle is drawn sample and is added in extraction reagent；
Reagent track will extract reagent and be pushed to sample extraction position and fixation；
The corresponding bar magnet for extracting position completes nucleic acid extraction work in corresponding position, and reagent is pushed to detection after having extracted
Storehouse, and close the door of extraction element；
It extracts reagent and pushes to detection device sample-adding position, detection reagent storehouse will test reagent and push to reagent track, sample
Needle draw extract nucleic acid be added detection reagent in, extract reagent by track push to inspection after sample storage warehouse；
Reagent transparent plastic after sample-adding is covered and is sent into detection device reaction tray and detects；Reaction tray be it is centrifugal,
Reaction system can be centrifuged and be mixed, can constantly resetted during the reaction, to carry out fluorescence detection；
It keeps temperature constant in detection process, and fluorescence detection is being carried out by CCD within a certain period of time；
Sample exhaust port is pushed out reaction tray after inspection after having detected.
Beneficial effects of the present invention:
1. the detection of nucleic acid quantification, qualitative, Genotyping and gene pleiomorphism can be carried out.
2. the continuous detection of sample of nucleic acid can be realized, the influence of not examined batch；
3. independent extract sample, it can be achieved that a variety of different type samples extract simultaneously, DNA or RNA can also be extracted simultaneously；
4. integrated nucleic acid extraction and detection of nucleic acids reduce the requirement of lab space in one；
5. pollution of nucleic acid risk can utmostly be reduced by being equipped with ultraviolet lamp and exhaust equipment；
Detection process whole-course automation can reduce technical threshold, be conducive to this technology and promote to base.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, will be described below to embodiment required
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 is the structural schematic diagram described in the embodiment of the present invention for nucleic acid extraction and the integration apparatus of detection；
Fig. 2 is the structural schematic diagram of nucleic acid-extracting apparatus described in the embodiment of the present invention；
Fig. 3 is the structural schematic diagram of nucleic acid-extracting apparatus described in the embodiment of the present invention；
Fig. 4 is the floor map of nucleic acid extraction unit described in the embodiment of the present invention；
Fig. 5 is the positive structure schematic of nucleic acid-extracting apparatus described in the embodiment of the present invention；
Fig. 6 is the structural schematic diagram on nucleic acid extraction unit upper layer described in the embodiment of the present invention；
Fig. 7 is the structural schematic diagram of detection device described in the embodiment of the present invention；
The floor map of detection device described in the embodiment of the present invention of the position Fig. 8；
The structural schematic diagram of reaction tray described in the embodiment of the present invention of the position Fig. 9；
In attached drawing, parts list represented by the reference numerals are as follows:
1- nucleic acid-extracting apparatus, 2- detection device, 3- controller, 4- injection port, 5- sample introduction track, 6- sample blending dress
It sets, 7- extraction agent bin, 8-TIP storehouses, 9- nucleic acid extraction unit, 10- bar magnet moving arm, 11- goes out sample track, and 12- mark product are deposited
Put area, 13- barcode reader, 14- sample needle, 15- extracts reagent suction needle, 16- reagent track, 17- branching agent track,
18- waste holder, 19- bar magnet, 20- ultraviolet lamp, 21- extraction position, 22-TIP loading channels, 23- reagent storage storehouse, 24- are discarded
TIP box, 25- reaction tray, 26- sample exhaust port, 27- detection detection reagent sample introduction rail, 28- propeller, 29- sample storage warehouse,
30- bottle capper, 31- reaction cup, 32- liquid injection port, 33- temperature detection mouth, 34- exhaust equipment, 35- reaction reagent storehouse, 36-
CCD device.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
As shown in figures 1-8
A kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, including nucleic acid-extracting apparatus 1, detection device
2 and controller 3, nucleic acid-extracting apparatus 1 is connected with detection device 2 and is equipped with gas-tight silo, and controller 3 is installed in 2 side of detection device
And it connects to power supply；
The nucleic acid-extracting apparatus 1 includes sample introduction track 5, reagent rail 16, nucleic acid extraction unit 9 and sample track out
11, the injection port of the sample introduction track 5 connects specimen holder propeller, and rear side installs sample blending device 6, and connects reagent track
Road 16,16 two sides of reagent rail separately design the reagent needle controlled by mechanical arm and specimen needle 14, and are correspondingly arranged extraction
The storehouse agent bin 7, TIP 8 and waste holder, 16 end of reagent rail connect nucleic acid extraction unit by branching agent rail 17
9, it is reagent rail 16 among the nucleic acid extraction unit 9, two sides are to extract position 21, the upper layer installation of nucleic acid extraction unit 9
The bar magnet 19 of specification arrangement is simultaneously controlled by bar magnet moving arm, and 9 rear end of nucleic acid extraction unit connects out sample track 11, out sample track
11 ends are the sample holding region 12 after extracting；
The detection device 2 includes the CCD device 36 of reaction tray 25 and ccd image sensor, the reaction
Multiple reaction cups 31, and design sample outlet 26 and detection reagent sample introduction rail 27, sample exhaust port 26 and inspection are housed on disk 25
Handgrip is designed in test agent sample introduction rail 27 and 25 connecting pin of reaction tray, and sample exhaust port 26 connects sample waste storage storehouse, detection examination
Sample bottle capper 30 is installed on agent sample introduction track 27, liquid injection port 32 and temperature detection mouth 33, CCD dress are designed in reaction tray 25
36 are set for fluorescence detection.
The bottom of the nucleic acid-extracting apparatus 1 is discarded TIP box 24, and upper end connects TIP channels 22 and reagent storage storehouse
23, ultraviolet lamp 20 is installed on 9 position of nucleic acid extraction unit and nucleic acid detection apparatus right side.
It TIP storehouses 8 in the detection device 2, reaction reagent storehouse 35 and the specimen needle 14 controlled by mechanical arm and pushes away
Into device；
35, reaction reagent storehouse refrigeration warehouse.
A kind of full-automatic nucleic acid amplification assays instrument control system based on isothermal amplification reactions, including controller, the control
Device processed includes detection unit, control unit, execution unit and display unit, and detection unit includes that the bar code of sample sample introduction track is read
Device, reaction tray temperature measuring probe are read, control unit and display unit are the main control chip and display of installation on the controller,
Execution unit includes that control mechanical arm, sample introduction track, reagent track, the sample of reagent needle and specimen needle go out sample track, bar magnet moves
Swing arm and the handgrip of detection device, mechanical arm；Barcode reader detection signal feeds back to control unit, and control unit driving executes
Mark product are sent to nucleic acid extraction unit by the sample sample introduction track of element, and drive control mechanical arm, reagent track and bar magnet move
Swing arm carries out nucleic acid extraction, and driving sample goes out sample track and send sample to storage area after the completion of extracting, and drives detection device later
Handgrip, mechanical arm detected.
Controller control specimen needle 14, reagent needle and bar magnet do not conflict, that is, can also mention in nucleic acid extraction to be other
Take plate reagent adding and sample again.
Embodiment 2
As shown in figures 1-8
A method of the nucleic acid extraction and detection of the full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, including
Following steps:
Specimen holder to be measured send to injection port 4 to wait and be loaded through sample introduction track, and identifies specimen information through barcode reader 13,
If necessary to mixing sample then mark product evenly mixing device 6 through rotation mix after be loaded again, after be loaded through out sample track send to
Sample storage area 12 after detection；
It extracts reagent and pushes to reagent track 16 from agent bin, and add other additional now with reagent；
Sample needle 14 is drawn sample and is added in extraction reagent；
Reagent track 16 will extract reagent and be pushed to nucleic acid extraction position 21 and fix；
The bar magnet 19 that corresponding core extracts position 21 completes nucleic acid extraction work in corresponding position, pushes to reagent after having extracted
Storehouse is detected, and closes extraction door；
Extract reagent push to detection device sample-adding position, detection reagent storehouse will test reagent (including reaction enzymes, buffer,
DNTP, primer etc.) push to reagent track 16, specimen needle 14 is drawn the nucleic acid extracted and is added in detection reagent, extract reagent by
Track pushes to sample storage warehouse 29 after inspection；
Reagent transparent plastic after sample-adding is covered and is sent into detection device reaction tray 25 and detects；
Sample to be examined reacts under constant temperature conditions (65 DEG C), and carries out fluorescence detection by CCD, and reaction tray is centrifugation
Formula, it can be ensured that reaction system mixes.Reaction tray is resetted per minute and carries out first order fluorescence detection；
Sample is pushed out reaction tray 25 by sample exhaust port 26 after inspection after having detected.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (6)
1. a kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, it is characterised in that: including nucleic acid-extracting apparatus,
Detection device and controller, nucleic acid-extracting apparatus are connected with detection device and are respectively equipped with gas-tight silo, detection device and extraction dress
Set respectively with controller and connect to power supply；
The nucleic acid-extracting apparatus includes that sample sample introduction track, reagent track, nucleic acid extraction unit and sample go out sample track, described
The injection port of sample sample introduction track connects specimen holder propeller, and rear side installs sample blending device, and connects reagent track, reagent
Track two sides separately design by mechanical arm control reagent suction needle and sample suction needle, and be correspondingly arranged extract agent bin,
TIP storehouses and waste holder, reagent track end connect each nucleic acid extraction unit, the nucleic acid extraction list by branching agent track
It is reagent track among first, track two sides are nucleic acid extraction position, and the bar magnet of the upper layer installation specification arrangement of nucleic acid extraction unit is simultaneously
It is controlled by bar magnet moving arm, nucleic acid extraction unit rear end connection sample goes out sample track, and sample track end is the mark after extracting out
Product storage area；
The detection device includes reaction tray and ccd image sensor (CCD) device, is equipped in the reaction tray
Multiple reaction cups, and design sample outlet and detection reagent sample introduction rail, sample exhaust port and detection reagent sample introduction track and anti-
Connecting pin design mechanical gripper should be coiled, sample exhaust port connects sample waste storage storehouse, installs sample on detection reagent sample introduction track
This bottle capper, liquid injection port and temperature detection mouth are designed in reaction tray, and CCD device is used for fluorescence detection.
2. a kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions as described in claim 1, it is characterised in that:
The nucleic acid-extracting apparatus upper end connects TIP loading channels and reagent storage storehouse, the nucleic acid extraction unit and detection unit
Ultraviolet lamp is installed.
3. a kind of full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions as described in claim 1, it is characterised in that:
TIP storehouses, agent bin and sample suction needle and propeller by mechanical arm control in the detection device；
Exhaust equipment is equipped on rear side of nucleic acid-extracting apparatus and nucleic acid detection apparatus.
The reagent position in storehouse refrigeration warehouse.
4. a kind of control system of the full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions as described in claim 1-3
System, it is characterised in that: the control system includes controller, and the controller includes detection unit, control unit, execution unit
And display unit, detection unit include the barcode reader of sample introduction track, reaction tray temperature measuring probe, control unit and display
Unit is the main control chip and display for being mounted on controller, execution unit include reagent needle and specimen needle control mechanical arm,
Sample sample introduction track, reagent track, sample go out sample track, the handgrip of bar magnet moving arm and detection device, mechanical arm；Bar code reading
Device detection signal feeds back to control unit, and sample is sent to nucleic acid and mentioned by the sample sample introduction track of control unit driving executive component
Unit is taken, and drive control mechanical arm, reagent track and bar magnet moving arm carry out nucleic acid extraction, driving sample goes out after the completion of extracting
Sample track send mark product to storage area, drives handgrip, the mechanical arm of detection device to be detected later.
5. the control system of the full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions as claimed in claim 4, special
Sign is: controller control specimen needle, reagent needle and bar magnet do not conflict, that is, can also be other extraction positions in nucleic acid extraction
Reagent adding and sample again.
6. a kind of method of the nucleic acid extraction and detection of the full-automatic nucleic acid amplification assays instrument based on isothermal amplification reactions, feature
It is: the following steps are included:
Specimen holder to be checked send to injection port to wait and be loaded through sample sample introduction track, and identifies specimen information through barcode reader, such as
Fruit needs the sample mixed to be then loaded again after rotation mixes in mark product evenly mixing device, and sample-adding goes out sample track through sample after completing and send
Sample storage area after to detection；
It extracts reagent and pushes to reagent track from agent bin, and add other additional now with reagent；
Sample needle is drawn sample and is added in extraction reagent；
Reagent track will extract reagent and be pushed to sample extraction position and fixation；
The corresponding bar magnet for extracting position completes nucleic acid extraction work in corresponding position, and reagent is pushed to detection storehouse after having extracted, and
Close the door of extraction element；
It extracts reagent and pushes to detection device sample-adding position, detection reagent storehouse will test reagent and push to reagent track, and specimen needle is inhaled
Take extraction nucleic acid be added detection reagent in, extract reagent by track push to inspection after sample storage warehouse；
Reagent transparent plastic after sample-adding is covered and is sent into detection device reaction tray and detects；Reaction tray be it is centrifugal, can will
Reaction system centrifugation mixes, and can constantly reset during the reaction, to carry out fluorescence detection；
It keeps temperature constant in detection process, and fluorescence detection is being carried out by CCD within a certain period of time；
Sample exhaust port is pushed out reaction tray after inspection after having detected.
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