CN106929406A - For nucleic acid extraction and the compact device for detecting - Google Patents
For nucleic acid extraction and the compact device for detecting Download PDFInfo
- Publication number
- CN106929406A CN106929406A CN201710366497.2A CN201710366497A CN106929406A CN 106929406 A CN106929406 A CN 106929406A CN 201710366497 A CN201710366497 A CN 201710366497A CN 106929406 A CN106929406 A CN 106929406A
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- nucleic acid
- detection
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- acid extraction
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 17
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 17
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 17
- 238000000605 extraction Methods 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 46
- 238000001821 nucleic acid purification Methods 0.000 claims abstract description 11
- 230000009897 systematic effect Effects 0.000 claims abstract description 5
- 238000000746 purification Methods 0.000 claims abstract description 3
- 238000007689 inspection Methods 0.000 abstract description 3
- 230000010354 integration Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the measure or method of inspection technical field of microorganism, more particularly to a kind of compact device for nucleic acid extraction and detection.This is used for nucleic acid extraction includes control unit with the compact device of detection（1）, movement unit（2）, sample pretreating unit（3）, detection unit（4）, control unit（1）For the storage of man-machine mutual friendship, systematic parameter and data, movement unit（2）Including the mechanical arm with porous plate handgrip, sample pretreating unit（3）For by the pretreatment of nucleic acid purification and/or sample in sample, detection unit（4）Detected for the sample after purification, detection unit（4）Positioned at sample pretreating unit（3）Lower section.This on the premise of small volume is increased, realizes the integration between the sample pretreating unit such as detection unit and nucleic acid purification unit for nucleic acid extraction and the compact device of detection.
Description
Technical field
The invention belongs to the measure or method of inspection technical field of microorganism, more particularly to a kind of nucleic acid extraction that is used for is with inspection
The compact device of survey.
Background technology
The innovation and creation of Application No. 201610575345.9 disclose a kind of automation microbial molecules testing equipment, its
Including control unit, movement unit, sample pretreatment unit, nucleic acid purification unit, detection unit, control unit is used for man-machine mutual
Hand over, the storage of systematic parameter and data, movement unit is mechanical arm, sample pretreatment unit include pretreating reagent storage area,
Separation module, by by the nucleic acid purification in pretreated sample, detection unit is to the sample after purification for nucleic acid purification unit
Detected, and obtained a result.The program can realize the ortho statesization detection of sample, but there is following defect：Sample pretreatment list
Unit, nucleic acid purification unit, detection unit are located in same level so that the space shared by equipment is larger.Commercially,
The big shot such as Tecan company provide platform is stable, mechanical arm movement is accurate reliable, be that the first-selection that automatic nucleic acid is extracted is put down
Platform.During the technical scheme of our company's application 201610575345.9, first it is envisioned that the side addition one in Tecan platforms is examined
Unit is surveyed, goes to destroy the ripe platforms of Tecan rather than selection.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided it is a kind of for nucleic acid extraction with detection compact
Equipment, it is realized between the sample pretreating unit such as detection unit and nucleic acid purification unit on the premise of small volume is increased
Integration.
To achieve these goals, technical scheme is as follows：For nucleic acid extraction and the compact device for detecting,
Including control unit, movement unit, sample pretreating unit, detection unit, control unit is used for man-machine mutual friendship, systematic parameter and number
According to storage, movement unit includes the mechanical arm with porous plate handgrip, and sample pretreating unit is used for the nucleic acid purification in sample
And/or the pretreatment of sample, detection unit be used for purify after sample detected, the detection unit be located at sample pretreating list
The lower section of unit.
Used as improvement, the sample pretreating unit is arranged on the upper surface of an equipment platform, and equipment platform is offered for many
The gateway that orifice plate comes in and goes out, the sample cell of detection unit is located at the lower section of gateway.
Used as improvement, the detection unit is PCR instrument.
As a further improvement, being provided with porous plate ejecting mechanism in the detection unit.
As a further improvement, the equipment is additionally provided with porous plate outlet.
As a further improvement, the both sides of the porous plate outlet set limited location guardrail.
The beneficial effects of the invention are as follows：The sample treatment such as nucleic acid purification unit unit is located on equipment platform, in order that real
Human users' facility is tested, the equipment platform has certain height in itself.This programme highly, detection unit is arranged on using this
The lower section of equipment platform, then for existing equipment platform, existing equipment platform need to only provide an area slightly larger than many
The plane space of orifice plate, digs out a gateway come in and gone out for porous plate, it is possible to realize nucleic acid extraction using the plane space
Platform is connected with detection unit.After porous plate completes detection in detection unit, porous plate is pushed away by porous plate ejecting mechanism
Go out detection unit, the mechanical arm with porous plate handgrip moves it to porous plate outlet again, experimenter will from porous plate exit
Porous plate is taken away.In the prior art, it is necessary to by hand take out the porous plate completed after detection from detection unit, movement unit one
Denier failure or program set improper, and movement unit meeting fast ram stretches into the arm of equipment, so as to be damaged to arm.And this
Scheme can automatically raise porous plate due to the presence of porous plate ejecting mechanism, then by the mechanical arm with porous plate handgrip by its
Porous plate outlet is moved to, treatment effeciency is not only increased, and it is safer.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the schematic diagram of porous plate ejecting mechanism in detection unit of the present invention；
Fig. 3 is the structural representation of porous plate outlet of the present invention.
In figure：1st, control unit；2nd, movement unit；3rd, sample pretreating unit；4th, detection unit；5th, equipment platform；51st, go out
Entrance；6th, porous plate ejecting mechanism；7th, porous plate outlet；8th, spacing guardrail.
Specific embodiment
Embodiment 1
As shown in figure 1, for nucleic acid extraction and the compact device for detecting, including control unit 1, movement unit 2, sample pretreating
Unit 3, detection unit 4, control unit 1 are used for the storage of man-machine mutual friendship, systematic parameter and data, and movement unit 2 includes that band is more
The mechanical arm of orifice plate handgrip, sample pretreating unit 3 is used for the pretreatment of nucleic acid purification and/or sample in sample, and detection is single
The sample that unit 4 is used for after purifying is detected that detection unit 4 is PCR instrument.
Detection unit 4 is located at the lower section of sample pretreating unit 3.Sample pretreating unit 3 is arranged on the upper table of an equipment platform 5
Face, equipment platform 5 offers the gateway 51 come in and gone out for porous plate, and the sample cell of detection unit 4 is located at the lower section of gateway 51.
As shown in Fig. 2 being provided with porous plate ejecting mechanism 6 in detection unit 4, porous plate ejecting mechanism 6 is a flexible bottom
Seat, its bottom for being located at PCR instrument sample cell is convenient to raise porous plate.
As shown in figure 3, the equipment is additionally provided with porous plate outlet 7, porous plate outlet 7 is located on the outer wall of equipment, porous
The both sides of plate outlet 7 set limited location guardrail 8.
Claims (6)
1. the compact device of nucleic acid extraction and detection, including control unit are used for（1）, movement unit（2）, sample pretreating unit
（3）, detection unit（4）, control unit（1）For the storage of man-machine mutual friendship, systematic parameter and data, movement unit（2）Including
Mechanical arm with porous plate handgrip, sample pretreating unit（3）For by the pretreatment of nucleic acid purification and/or sample in sample,
Detection unit（4）Detected for the sample after purification, it is characterised in that：The detection unit（4）Positioned at sample pretreating list
Unit（3）Lower section.
2. the compact device of nucleic acid extraction and detection is used for as claimed in claim 1, it is characterised in that：The sample pretreating
Unit（3）It is arranged on an equipment platform（5）Upper surface, equipment platform（5）Open up the gateway for being equipped with and being come in and gone out for porous plate
（51）, detection unit（4）Sample cell be located at gateway（51）Lower section.
3. the compact device of nucleic acid extraction and detection is used for as claimed in claim 1, it is characterised in that：The detection unit
（4）It is PCR instrument.
4. the compact device of nucleic acid extraction and detection is used for as claimed in claim 1, it is characterised in that：The detection
Unit（4）Inside it is provided with porous plate ejecting mechanism（6）.
5. the compact device of nucleic acid extraction and detection is used for as claimed in claim 1, it is characterised in that：The equipment also sets up
There is porous plate to export（7）.
6. the compact device of nucleic acid extraction and detection is used for as claimed in claim 5, it is characterised in that：The porous plate goes out
Mouthful（7）Both sides set limited location guardrail（8）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366497.2A CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366497.2A CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106929406A true CN106929406A (en) | 2017-07-07 |
| CN106929406B CN106929406B (en) | 2024-02-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710366497.2A Active CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
Country Status (1)
| Country | Link |
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| CN (1) | CN106929406B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642046A (en) * | 2018-05-16 | 2018-10-12 | 上海科源电子科技有限公司 | Integral biological sample treatment and detection device and its method |
| CN113173399A (en) * | 2021-03-15 | 2021-07-27 | 北京戴纳实验科技有限公司 | Transport case nucleic acid detecting system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2827873A1 (en) * | 2001-07-24 | 2003-01-31 | Amenagement Urbain & Rural | Process for detecting presence of pathogenic organisms in water sample, by concentrating pathogenic organisms, opening cells of pathogenic organisms, amplifying nucleic acid and detecting DNA and/or RNA |
| JP2012100549A (en) * | 2010-11-08 | 2012-05-31 | Hitachi High-Technologies Corp | Nucleic acid analysis device |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN105403713A (en) * | 2015-12-09 | 2016-03-16 | 中国科学院苏州生物医学工程技术研究所 | A microplate centrifuge with a tray position adjusting mechanism |
| CN106190816A (en) * | 2016-07-19 | 2016-12-07 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection equipment |
| CN205803484U (en) * | 2016-07-19 | 2016-12-14 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection equipment |
| CN206768120U (en) * | 2017-05-23 | 2017-12-19 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the compact device of detection |
-
2017
- 2017-05-23 CN CN201710366497.2A patent/CN106929406B/en active Active
Patent Citations (8)
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|---|---|---|---|---|
| FR2827873A1 (en) * | 2001-07-24 | 2003-01-31 | Amenagement Urbain & Rural | Process for detecting presence of pathogenic organisms in water sample, by concentrating pathogenic organisms, opening cells of pathogenic organisms, amplifying nucleic acid and detecting DNA and/or RNA |
| JP2012100549A (en) * | 2010-11-08 | 2012-05-31 | Hitachi High-Technologies Corp | Nucleic acid analysis device |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642046A (en) * | 2018-05-16 | 2018-10-12 | 上海科源电子科技有限公司 | Integral biological sample treatment and detection device and its method |
| CN113173399A (en) * | 2021-03-15 | 2021-07-27 | 北京戴纳实验科技有限公司 | Transport case nucleic acid detecting system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106929406B (en) | 2024-02-06 |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200909 Address after: 1213-177, 12 / F, building 3, No. 1366, Hongfeng Road, Kangshan street, Huzhou City, Zhejiang Province Applicant after: Original microbial detection technology (Huzhou) Co.,Ltd. Address before: 313008 Zhejiang Province, Huzhou city Wuxing District District Road No. 1188 in the eastern city of Huzhou Metro headquarters free port 11 building H No. 15-16 Applicant before: HUZHOU HIGH-INNO BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20240112 Address after: Room 293, 2nd Floor, Zitian Building, No. 8 Xiyuan 5th Road, Sandun Town, Xihu District, Hangzhou City, Zhejiang Province, 310030 Applicant after: Zhinong Technology Development (Zhejiang) Co.,Ltd. Address before: Room 1213-177, 12th Floor, Building 3, No. 1366 Hongfeng Road, Kangshan Street, Huzhou City, Zhejiang Province, 313000 Applicant before: Original microbial detection technology (Huzhou) Co.,Ltd. |
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