CN106929406B - Compact device for nucleic acid extraction and detection - Google Patents
Compact device for nucleic acid extraction and detection Download PDFInfo
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- CN106929406B CN106929406B CN201710366497.2A CN201710366497A CN106929406B CN 106929406 B CN106929406 B CN 106929406B CN 201710366497 A CN201710366497 A CN 201710366497A CN 106929406 B CN106929406 B CN 106929406B
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- 238000001514 detection method Methods 0.000 title claims abstract description 42
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 19
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 19
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 title claims abstract description 9
- 239000000523 sample Substances 0.000 claims abstract description 36
- 230000003993 interaction Effects 0.000 claims abstract description 5
- 239000012521 purified sample Substances 0.000 claims abstract description 5
- 238000007781 pre-processing Methods 0.000 claims abstract description 4
- 238000001821 nucleic acid purification Methods 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000010354 integration Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 230000007547 defect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of a method for measuring or detecting microorganisms, and particularly relates to compact equipment for extracting and detecting nucleic acid. The compact device for nucleic acid extraction and detection comprises a control unit (1), a transfer unit (2), a sample processing unit (3) and a detection unit (4), wherein the control unit (1) is used for man-machine interaction and storage of system parameters and data, the transfer unit (2) comprises a mechanical arm with a porous plate gripper, the sample processing unit (3) is used for purifying nucleic acid in a sample and/or preprocessing the sample, the detection unit (4) is used for detecting the purified sample, and the detection unit (4) is located below the sample processing unit (3). The compact device for nucleic acid extraction and detection realizes the integration between the detection unit and the sample processing unit such as the nucleic acid purification unit on the premise of small volume increase.
Description
Technical Field
The invention belongs to the technical field of a method for measuring or detecting microorganisms, and particularly relates to compact equipment for extracting and detecting nucleic acid.
Background
The invention of application number 201610575345.9 discloses automatic microorganism molecule detection equipment, which comprises a control unit, a transfer unit, a sample pretreatment unit, a nucleic acid purification unit and a detection unit, wherein the control unit is used for man-machine interaction and storage of system parameters and data, the transfer unit is a mechanical arm, the sample pretreatment unit comprises a pretreatment reagent storage area and a separation module, the nucleic acid purification unit is used for purifying nucleic acid in a pretreated sample, and the detection unit is used for detecting the purified sample and obtaining a result. The scheme can realize the original state detection of the sample, but has the following defects: the sample pretreatment unit, the nucleic acid purification unit and the detection unit are positioned on the same horizontal plane, so that the space occupied by the equipment is larger. In the market, a platform provided by Tecan and other big-brand companies runs stably, and a mechanical arm moves accurately and reliably, so that the platform is a preferred platform for automatic nucleic acid extraction. In the technical solution of the present company application 201610575345.9, it is first conceivable to add a detection unit to the side of the Tecan platform instead of choosing to destroy the Tecan mature platform.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide compact equipment for extracting and detecting nucleic acid, which realizes the integration between a detection unit and a sample processing unit such as a nucleic acid purification unit and the like on the premise of small increase of volume.
In order to achieve the above object, the technical scheme of the present invention is as follows: the compact equipment for extracting and detecting nucleic acid comprises a control unit, a transfer unit, a sample processing unit and a detection unit, wherein the control unit is used for man-machine interaction and storage of system parameters and data, the transfer unit comprises a mechanical arm with a porous plate gripper, the sample processing unit is used for purifying nucleic acid in a sample and/or preprocessing the sample, the detection unit is used for detecting the purified sample, and the detection unit is positioned below the sample processing unit.
As an improvement, the sample processing unit is arranged on the upper surface of an equipment platform, the equipment platform is provided with an inlet and an outlet for the porous plate to come in and go out, and the sample cell of the detection unit is positioned below the inlet and the outlet.
As an improvement, the detection unit is a PCR instrument.
As a further improvement, a perforated plate pushing mechanism is arranged in the detection unit.
As a further improvement, the apparatus is also provided with a perforated plate outlet.
As a further improvement, two sides of the outlet of the porous plate are provided with limiting guardrails.
The beneficial effects of the invention are as follows: the sample processing units such as the nucleic acid purification unit are positioned on the equipment platform, and the equipment platform has a certain height for the convenience of operation of experimenters. This scheme utilizes this height, sets up detecting element in the below of equipment platform, then to current equipment platform, current equipment platform only need provide a area slightly more than the plane space of perforated plate, utilizes this plane space to dig out an access & exit that supplies the perforated plate to come in and go out, just can realize the linking of nucleic acid extraction platform and detecting element. After the porous plate is detected in the detection unit, the porous plate is pushed out of the detection unit by the porous plate pushing mechanism, the mechanical arm with the porous plate gripper moves the porous plate to the porous plate outlet, and an experimenter takes away the porous plate from the porous plate outlet. In the prior art, the perforated plate after detection needs to be manually taken out from the detection unit, and once the moving unit fails or the program is improperly set, the moving unit can quickly strike the arm extending into the equipment, so that the arm is damaged. The scheme is characterized in that the porous plate pushing mechanism is arranged, so that the porous plate can be automatically lifted, and then the mechanical arm with the porous plate gripper moves the porous plate to the porous plate outlet, so that the treatment efficiency is improved, and the porous plate pushing mechanism is safer.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of a porous plate pushing mechanism in the detecting unit of the present invention;
FIG. 3 is a schematic view of the structure of the outlet of the perforated plate according to the present invention.
In the figure: 1. a control unit; 2. a transporting unit; 3. a sample processing unit; 4. a detection unit; 5. an equipment platform; 51. an access opening; 6. a perforated plate pushing mechanism; 7. a porous plate outlet; 8. limiting guardrails.
Detailed Description
Example 1
As shown in fig. 1, the compact device for nucleic acid extraction and detection comprises a control unit 1, a transfer unit 2, a sample processing unit 3 and a detection unit 4, wherein the control unit 1 is used for man-machine interaction and storage of system parameters and data, the transfer unit 2 comprises a mechanical arm with a porous plate gripper, the sample processing unit 3 is used for purifying nucleic acid in a sample and/or preprocessing the sample, the detection unit 4 is used for detecting the purified sample, and the detection unit 4 is a PCR instrument.
The detection unit 4 is located below the sample processing unit 3. The sample processing unit 3 is disposed on an upper surface of an apparatus platform 5, the apparatus platform 5 is provided with an inlet 51 through which a porous plate is inserted and removed, and a sample cell of the detection unit 4 is disposed below the inlet 51.
As shown in FIG. 2, a porous plate pushing mechanism 6 is arranged in the detection unit 4, and the porous plate pushing mechanism 6 is a telescopic base which is positioned at the bottom of a sample cell of the PCR instrument, so that the porous plate can be conveniently lifted.
As shown in fig. 3, the device is further provided with a perforated plate outlet 7, the perforated plate outlet 7 is positioned on the outer wall of the device, and two sides of the perforated plate outlet 7 are provided with limit guardrails 8.
Claims (3)
1. The compact equipment for extracting and detecting nucleic acid comprises a control unit (1), a transporting unit (2), a sample processing unit (3) and a detection unit (4), wherein the control unit (1) is used for man-machine interaction and storage of system parameters and data, the transporting unit (2) comprises a mechanical arm with a porous plate gripper, the sample processing unit (3) is used for purifying nucleic acid in a sample and/or preprocessing the sample, and the detection unit (4) is used for detecting the purified sample and is characterized in that: the sample processing unit (3) is arranged on the upper surface of an equipment platform (5), the equipment platform (5) is provided with an inlet and outlet (51) for a porous plate to go in and out, and a sample cell of the detection unit (4) is positioned below the inlet and outlet (51) so that the detection unit (4) is positioned below the sample processing unit (3); the detection unit (4) is a PCR instrument, and a porous plate pushing mechanism (6) is arranged in the detection unit (4) and used for pushing the porous plate out of the detection unit (4).
2. The compact device for nucleic acid extraction and detection of claim 1, wherein: the apparatus is also provided with a perforated plate outlet (7).
3. The compact device for nucleic acid extraction and detection of claim 2, wherein: limiting guardrails (8) are arranged on two sides of the porous plate outlet (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366497.2A CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366497.2A CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106929406A CN106929406A (en) | 2017-07-07 |
| CN106929406B true CN106929406B (en) | 2024-02-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710366497.2A Active CN106929406B (en) | 2017-05-23 | 2017-05-23 | Compact device for nucleic acid extraction and detection |
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| CN (1) | CN106929406B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642046A (en) * | 2018-05-16 | 2018-10-12 | 上海科源电子科技有限公司 | Integral biological sample treatment and detection device and its method |
| CN113173399B (en) * | 2021-03-15 | 2022-07-29 | 北京戴纳实验科技有限公司 | Transport case nucleic acid detecting system |
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|---|---|---|---|---|
| FR2827873A1 (en) * | 2001-07-24 | 2003-01-31 | Amenagement Urbain & Rural | Process for detecting presence of pathogenic organisms in water sample, by concentrating pathogenic organisms, opening cells of pathogenic organisms, amplifying nucleic acid and detecting DNA and/or RNA |
| JP2012100549A (en) * | 2010-11-08 | 2012-05-31 | Hitachi High-Technologies Corp | Nucleic acid analysis device |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
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| CN106190816A (en) * | 2016-07-19 | 2016-12-07 | 湖州高亿诺生物科技有限公司 | Automatization's microbial molecules detection equipment |
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| CN206768120U (en) * | 2017-05-23 | 2017-12-19 | 湖州高亿诺生物科技有限公司 | For nucleic acid extraction and the compact device of detection |
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2017
- 2017-05-23 CN CN201710366497.2A patent/CN106929406B/en active Active
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| FR2827873A1 (en) * | 2001-07-24 | 2003-01-31 | Amenagement Urbain & Rural | Process for detecting presence of pathogenic organisms in water sample, by concentrating pathogenic organisms, opening cells of pathogenic organisms, amplifying nucleic acid and detecting DNA and/or RNA |
| JP2012100549A (en) * | 2010-11-08 | 2012-05-31 | Hitachi High-Technologies Corp | Nucleic acid analysis device |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN105403713A (en) * | 2015-12-09 | 2016-03-16 | 中国科学院苏州生物医学工程技术研究所 | A microplate centrifuge with a tray position adjusting mechanism |
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| CN106929406A (en) | 2017-07-07 |
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Effective date of registration: 20200909 Address after: 1213-177, 12 / F, building 3, No. 1366, Hongfeng Road, Kangshan street, Huzhou City, Zhejiang Province Applicant after: Original microbial detection technology (Huzhou) Co.,Ltd. Address before: 313008 Zhejiang Province, Huzhou city Wuxing District District Road No. 1188 in the eastern city of Huzhou Metro headquarters free port 11 building H No. 15-16 Applicant before: HUZHOU HIGH-INNO BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20240112 Address after: Room 293, 2nd Floor, Zitian Building, No. 8 Xiyuan 5th Road, Sandun Town, Xihu District, Hangzhou City, Zhejiang Province, 310030 Applicant after: Zhinong Technology Development (Zhejiang) Co.,Ltd. Address before: Room 1213-177, 12th Floor, Building 3, No. 1366 Hongfeng Road, Kangshan Street, Huzhou City, Zhejiang Province, 313000 Applicant before: Original microbial detection technology (Huzhou) Co.,Ltd. |
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