CN104132834A - Dual-functional full-automatic cell sedimentation slide preparing machine and slide preparing method thereof - Google Patents
Dual-functional full-automatic cell sedimentation slide preparing machine and slide preparing method thereof Download PDFInfo
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- CN104132834A CN104132834A CN201410382486.XA CN201410382486A CN104132834A CN 104132834 A CN104132834 A CN 104132834A CN 201410382486 A CN201410382486 A CN 201410382486A CN 104132834 A CN104132834 A CN 104132834A
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Abstract
The invention discloses a dual-functional full-automatic cell sedimentation slide preparing machine and a slide preparing method thereof. The dual-functional full-automatic cell sedimentation slide preparing machine comprises a work platform (1), wherein a barrel removal working station (7) is arranged on one side, which is close to an upright (2), of the upper surface of the working platform (1), a reagent absorbing working station (8), a cell sedimentation slide preparing working station (9) and a DNA slide preparing working station (10) are sequentially arranged on the other side of the upper surface of the working platform (1), a sample reagent absorbing device is arranged on the reagent absorbing working station (8), a sedimentation bin base (26) is arranged on each of the cell sedimentation slide preparing working station (9) and the DNA slide preparing working station (10), and a liquid absorbing and injecting column (5) and a sample reagent control end (6) are respectively arranged at the bottom of a vertical movement arm (4) through a fixed frame. The dual-functional full-automatic cell sedimentation slide preparing machine is reasonable in structure, is capable of simultaneously achieving cell slide preparation and DNA slide preparation, and high in automation degree; and the fussy manual operation process is omitted, the slide preparing process is fully-automatically completed, the slide preparing efficiency is high, the slide preparing effect is improved, and the application range is wide.
Description
Technical field
The present invention relates to a kind of pelleter, relate in particular to a kind of double-function full automatic cell settlement pelleter and flaking method thereof, belong to technical field of medical instruments.
Background technology
At present, clinical medicine completes by a series of flow process cervical carcinoma early screening and DNA inspection, and wherein extracting sample and film-making is vital two links.Cervical cell film-making and DNA film-making utilize exactly uterus neck label taking this, being news media with reagent transfers to cell in sample on microslide, to form thin slice.In currently available technology, cervical cell film-making and DNA film-making, complete separately with cervical cell pelleter and DNA film-making respectively, function dullness, and complex operation, can not meet the needs of practical application.
Summary of the invention
First object of the present invention is simple in structure for existing cell chip preparing machine and DNA pelleter, function singleness, can not carry out sectioning cells and NDA film-making simultaneously, automaticity is lower, the defect that production effect is not good and deficiency, provide a kind of rational in infrastructure, functional diversities, can carry out sectioning cells and NDA film-making, a kind of double-function full automatic cell settlement pelleter that production effect is good simultaneously.
Second object of the present invention is that automaticity is lower for existing sectioning cells and NDA film-making complicated operation, cannot carry out defect and the deficiency of sectioning cells and NDA film-making simultaneously, and a kind of double-function full automatic cell settlement pelleter flaking method is provided.
For realizing first object of the present invention, technical solution of the present invention is: a kind of double-function full automatic cell settlement pelleter, comprise workbench, one side of workbench is vertically fixed with column, transverse arm is installed on horizontal guide rail on column, the outer end of transverse arm is provided with vertical movement arm, the upper surface of described workbench is provided with de-cylinder working position near a side of column, the opposite side of workbench upper surface is disposed with reagent and draws working position, cell settlement film-making working position and DNA film-making working position, on de-cylinder working position, de-frame is installed, the upper end of de-frame offers bayonet socket, reagent is drawn on working position and is provided with sample reagent liquid sucking device, on cell settlement film-making working position and DNA film-making working position, lay respectively settling bin seat, the upper surface of settling bin seat is provided with arc-shaped slot, on settling bin seat, be placed with settling bin, on the sidewall of settling bin near bottom, be provided with buckle corresponding to arc-shaped slot, buckle is stuck in the inside of arc-shaped slot, the bottom of vertical movement arm is separately installed with imbibition fluid injection post and sample reagent control end by fixed mount.
Described sample reagent liquid sucking device comprises glass stand, reagent cup, sample suction sleeve, step surface and imbibing hole, on glass stand, offer reagent cup putting hole, in reagent cup putting hole, be vertically placed with reagent cup, in reagent cup, be placed with sample and inhale sleeve, sample is inhaled on sleeve upper edge hole and is provided with step surface, step surface is shelved in the upper port of reagent cup, and the bottom that sample is inhaled sleeve offers imbibing hole.
Described imbibition fluid injection post is made up of straight tube post, imbibition passage, pipette, reservoir channel and liquid injection pipe, in straight tube post, offer respectively imbibition passage and reservoir channel, imbibition passage is connected with pipette, and reservoir channel is connected with liquid injection pipe, and the length of pipette is greater than the length of liquid injection pipe.
Described sample reagent control end is made up of cylindrical body, air flue, positive port, negative port and grommet type seals, in cylindrical body, offer two parallel air flues, the upper port of one of them air flue is positive port, the upper port of another air flue is negative port, and the outer round surface of cylindrical body is provided with grommet type seals.
At the bottom of the storehouse of described settling bin, be placed with microslide, be placed with sealing gasket on microslide, in the upper port of settling bin, be provided with filter mantle, the bottom of filter mantle is filtering membrane, and the outer round surface of filter mantle is provided with card step, and card step is shelved in the upper port of settling bin.
Described transverse arm is connected with controller respectively with vertical movement arm.
For realizing second object of the present invention, technical solution of the present invention is: a kind of double-function full automatic cell settlement pelleter flaking method, is characterized in that comprising the following steps:
A, film-making are prepared: inject appropriate sample reagent to reagent cup, on cell settlement film-making working position and DNA film-making working position, place respectively settling bin, settling bin is fixed on settling bin seat, places microslide, and filter mantle is placed in the upper port of settling bin in settling bin;
B, suck sample reagent: controller control transverse arm move to reagent draw working position directly over, simultaneously vertical movement arm reagent draw working position directly over after under stretch out sample reagent control end, draw the sample reagent in reagent cup in sample reagent liquid sucking device;
C, inject sample reagent to sectioning cells: vertical movement arm drives sample reagent control end to move upward to throw off contact, transverse arm move to cell settlement film-making working position directly over, vertical movement arm moves downward until sample reagent control end is positioned at 2 millimeters of-3 millimeters of places, filter mantle top stops subsequently, and the sample reagent of discharging half is in the settling bin on cell settlement film-making working position;
D, add sample reagent to DNA film-making: vertical movement arm drives sample reagent control end to move upward, transverse arm move to DNA film-making working position directly over, vertical movement arm moves downward until sample reagent control end is positioned at 2 millimeters of-3 millimeters of places, filter mantle top stops subsequently, discharges remaining half sample reagent in the settling bin on DNA film-making working position;
E, slough sample and inhale sleeve: transverse arm and vertical movement arm drive sample reagent control end run to de-cylinder working position directly over, utilize de-frame that sample is inhaled to sleeve and slough from the lower end of sample reagent control end;
F, slough filter mantle: transverse arm and vertical movement arm drive sample reagent control end run to successively cell settlement film-making working position and DNA film-making working position directly over, and sample reagent control end is inserted into respectively to the upper port inside of filter mantle, then with filter mantle run to de-cylinder working position directly over, utilize de-frame that filter mantle is sloughed;
G, exhaustion sample reagent waste liquid: after until cell, on microslide, sedimentation completes, transverse arm and vertical movement arm drive imbibition fluid injection post to run to the settling bin top on DNA film-making working position, imbibition fluid injection post blots sample reagent waste liquid only, then with the same manner, the sample reagent waste liquid in settling bin on cell settlement film-making working position is blotted only;
H, add ethanol: transverse arm and vertical movement arm drive imbibition fluid injection post to run to respectively the settling bin top on cell settlement film-making working position and DNA film-making working position, add respectively 90% ethanol immobile liquid, wait for that cell and DNA complete fixing;
I, exhaustion ethanol waste liquid: imbibition fluid injection post runs to respectively the settling bin top on cell settlement film-making working position and DNA film-making working position, and by the exhaustion of ethanol waste liquid, sectioning cells and DNA film-making complete respectively, take out sectioning cells and DNA film-making.
The invention has the beneficial effects as follows:
1, the present invention is disposed with de-cylinder working position on workbench, reagent is drawn working position, cell settlement film-making working position and DNA film-making working position, on de-cylinder working position, by taking off, frame can be inhaled sleeve by sample and filter mantle is taken off, reagent is drawn working position and is realized the absorption operation to sample reagent, and cell settlement film-making working position and DNA film-making working position can be realized respectively sectioning cells and DNA film-making.
2, the present invention is rational in infrastructure, can realize sectioning cells and DNA film-making simultaneously, and automaticity is high, has saved loaded down with trivial details manual operation engineering, and film-making process automatically completes, and film-making efficiency is high, and has improved production effect, and utilization scope is wide.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is the structural representation of imbibition fluid injection post of the present invention.
Fig. 3 is that the structure of showing of sample reagent control end of the present invention is intended to.
Fig. 4 is the structural representation of sample reagent liquid sucking device of the present invention.
Fig. 5 is the structural representation of settling bin seat of the present invention.
Fig. 6 is the structural representation of settling bin of the present invention.
Fig. 7 is the structural representation of the de-frame of the present invention.
In figure: workbench 1, column 2, transverse arm 3, vertical movement arm 4, imbibition fluid injection post 5, sample reagent control end 6, de-cylinder working position 7, reagent is drawn working position 8, cell settlement film-making working position 9, DNA film-making working position 10, straight tube post 11, imbibition passage 12, pipette 13, reservoir channel 14, liquid injection pipe 15, cylindrical body 16, air flue 17, positive port 18, negative port 19, grommet type seals 20, glass stand 21, reagent cup 22, sample is inhaled sleeve 23, step surface 24, imbibing hole 25, settling bin seat 26, settling bin 27, arc-shaped slot 28, buckle 29, filter mantle 30, filtering membrane 31, card step 32, microslide 33, sealing gasket 34, de-frame 35, bayonet socket 36, controller 37.
Embodiment
Below in conjunction with brief description of the drawings and embodiment, the present invention is described in further detail:
Referring to Fig. 1 to Fig. 7, a kind of double-function full automatic cell settlement pelleter of the present invention, comprise workbench 1, one side of workbench 1 is vertically fixed with column 2, transverse arm 3 is installed on horizontal guide rail on column 2, the outer end of transverse arm 3 is provided with vertical movement arm 4, the upper surface of described workbench 1 is provided with de-cylinder working position 7 near a side of column 2, the opposite side of workbench 1 upper surface is disposed with reagent and draws working position 8, cell settlement film-making working position 9 and DNA film-making working position 10, on de-cylinder working position 7, de-frame 35 is installed, the upper end of de-frame 35 offers bayonet socket 36, reagent is drawn on working position 8 and is provided with sample reagent liquid sucking device, on cell settlement film-making working position 9 and DNA film-making working position 10, lay respectively settling bin seat 26, the upper surface of settling bin seat 26 is provided with arc-shaped slot 28, on settling bin seat 26, be placed with settling bin 27, settling bin 27 is near being provided with buckle 29 corresponding to arc-shaped slot 28 on the sidewall of bottom, buckle 29 is stuck in the inside of arc-shaped slot 28, the bottom of vertical movement arm 4 is separately installed with imbibition fluid injection post 5 and sample reagent control end 6 by fixed mount.
Described sample reagent liquid sucking device comprises glass stand 21, reagent cup 22, sample suction sleeve 23, step surface 24 and imbibing hole 25, on glass stand 21, offer reagent cup putting hole, in reagent cup putting hole, be vertically placed with reagent cup 22, in reagent cup 22, be placed with sample and inhale sleeve 23, sample is inhaled on sleeve 23 upper edge holes and is provided with step surface 24, step surface 24 is shelved in the upper port of reagent cup 22, and the bottom that sample is inhaled sleeve 23 offers imbibing hole 25.
Described imbibition fluid injection post 5 is made up of straight tube post 11, imbibition passage 12, pipette 13, reservoir channel 14 and liquid injection pipe 15, interior imbibition passage 12 and the reservoir channel 14 of offering respectively of straight tube post 11, imbibition passage 12 is connected with pipette 13, reservoir channel 14 is connected with liquid injection pipe 15, and the length of pipette 13 is greater than the length of liquid injection pipe 15.
Described sample reagent control end 6 is made up of cylindrical body 16, air flue 17, positive port 18, negative port 19 and grommet type seals 20, in cylindrical body 16, offer two parallel air flues 17, the upper port of one of them air flue 17 is positive port 18, the upper port of another air flue 17 is negative port 19, and the outer round surface of cylindrical body 16 is provided with grommet type seals 20.
At the bottom of the storehouse of described settling bin 27, be placed with microslide 33, on microslide 33, be placed with sealing gasket 34, in the upper port of settling bin 27, be provided with filter mantle 30, the bottom of filter mantle 30 is filtering membrane 31, the outer round surface of filter mantle 30 is provided with card step 32, and card step 32 is shelved in the upper port of settling bin 27.
Described transverse arm 3 is connected with controller 37 respectively with vertical movement arm 4.
A kind of double-function full automatic cell settlement pelleter flaking method, comprises the following steps:
A, film-making are prepared: inject appropriate sample reagent to reagent cup 22, on cell settlement film-making working position 9 and DNA film-making working position 10, place respectively settling bin 27, settling bin 27 is fixed on settling bin seat 26, the interior placement microslide 33 of settling bin 27, and filter mantle 30 is placed in the upper port of settling bin 27;
B, suck sample reagent: controller 37 control transverse arm 3 move to reagent draw working position 8 directly over, simultaneously vertical movement arm 4 reagent draw working position 8 directly over after under stretch out sample reagent control end 6, draw the sample reagent in reagent cup 22 in sample reagent liquid sucking device;
C, inject sample reagent to sectioning cells: vertical movement arm 4 drives sample reagent control end 6 to move upward to throw off contact, transverse arm 3 move to cell settlement film-making working position 9 directly over, vertical movement arm 4 moves downward until sample reagent control end 6 is positioned at-3 millimeters of places, 2 millimeters of filter mantle 30 tops stops subsequently, and the sample reagent of discharging half is in the settling bin 27 on cell settlement film-making working position 9;
D, add sample reagent to DNA film-making: vertical movement arm 4 drives sample reagent control end 6 to move upward, transverse arm 3 move to DNA film-making working position 10 directly over, vertical movement arm 4 moves downward until sample reagent control end 6 is positioned at-3 millimeters of places, 2 millimeters of filter mantle 30 tops stops subsequently, discharges remaining half sample reagent in the settling bin 27 on DNA film-making working position 10;
E, slough sample and inhale sleeve: transverse arm 3 and vertical movement arm 4 drive sample reagent control end 6 run to de-cylinder working position 7 directly over, utilize de-frame 35 that sample is inhaled to sleeve 23 and slough from the lower end of sample reagent control end 6;
F, slough filter mantle: transverse arm 3 and vertical movement arm 4 drive sample reagent control end 6 run to successively cell settlement film-making working position 9 and DNA film-making working position 10 directly over, and sample reagent control end 6 is inserted into respectively to the upper port inside of filter mantle 30, then with filter mantle 30 run to de-cylinder working position 7 directly over, utilize de-frame 35 that filter mantle 30 is sloughed;
G, exhaustion sample reagent waste liquid: after until cell, on microslide 33, sedimentation completes, transverse arm 3 and vertical movement arm 4 drive imbibition fluid injection post 5 to run to settling bin 27 tops on DNA film-making working position 10, imbibition fluid injection post 5 blots sample reagent waste liquid only, then with the same manner, the sample reagent waste liquid in settling bin 27 on cell settlement film-making working position 9 is blotted only;
H, add ethanol: transverse arm 3 and vertical movement arm 4 drive imbibition fluid injection post 5 to run to respectively settling bin 27 tops on cell settlement film-making working position 9 and DNA film-making working position 10, add respectively 90% ethanol immobile liquid, wait for that cell and DNA complete fixing;
I, exhaustion ethanol waste liquid: imbibition fluid injection post 5 runs to respectively settling bin 27 tops on cell settlement film-making working position 9 and DNA film-making working position 10, and by the exhaustion of ethanol waste liquid, sectioning cells and DNA film-making complete respectively, take out sectioning cells and DNA film-making.
Referring to Fig. 1, a side of workbench 1 is vertically fixed with column 2, and transverse arm 3 is installed on horizontal guide rail on column 2, and the outer end of transverse arm 3 is provided with vertical movement arm 4, and transverse arm 3 is connected with controller 37 respectively with vertical movement arm 4.Controller 37 can be controlled the rotation of X-axis motor, thereby realize the motion control in X-direction to transverse arm 3, same controller 37 can the motion of vertical movement arm 4 in Y-axis, and vertical movement arm 4 can also be realized in the vertical direction accurately motion within the specific limits.The bottom of vertical movement arm 4 is separately installed with imbibition fluid injection post 5 and sample reagent control end 6 by fixed mount, the lower end of sample reagent control end 6 can be socketed sample and inhale sleeve 23, after socket sample is inhaled sleeve 23, can from reagent cup 22, extract easily sample reagent.
The upper surface of workbench 1 is provided with de-cylinder working position 7 near a side of column 2, and the opposite side of workbench 1 upper surface is disposed with reagent and draws working position 8, cell settlement film-making working position 9 and DNA film-making working position 10.On de-cylinder working position 7, de-frame 35 is installed, the upper end of de-frame 35 offers bayonet socket 36, sample can be inhaled to sleeve 23 and filter mantle 30 is taken off by bayonet socket 36, make to inhale sleeve 23 and depart from from the lower end of sample reagent control end 6, filter mantle 30 departs from from the upper port of settling bin 27.Reagent is drawn on working position 8 and is provided with sample reagent liquid sucking device, and sample reagent liquid sucking device comprises glass stand 21, reagent cup 22, sample suction sleeve 23, step surface 24 and imbibing hole 25.On glass stand 21, offer reagent cup putting hole, in reagent cup putting hole, be vertically placed with reagent cup 22, in reagent cup 22, be placed with sample and inhale sleeve 23, sample is inhaled on sleeve 23 upper edge holes and is provided with step surface 24, step surface 24 is shelved in the upper port of reagent cup 22, and the bottom that sample is inhaled sleeve 23 offers imbibing hole 25.
Imbibition fluid injection post 5 is made up of straight tube post 11, imbibition passage 12, pipette 13, reservoir channel 14 and liquid injection pipe 15, interior imbibition passage 12 and the reservoir channel 14 of offering respectively of straight tube post 11, imbibition passage 12 is connected with pipette 13, reservoir channel 14 is connected with liquid injection pipe 15, and the length of pipette 13 is greater than the length of liquid injection pipe 15.Sample reagent control end 6 is made up of cylindrical body 16, air flue 17, positive port 18, negative port 19 and grommet type seals 20, in cylindrical body 16, offer two parallel air flues 17, the upper port of one of them air flue 17 is positive port 18, the upper port of another air flue 17 is negative port 19, and the outer round surface of cylindrical body 16 is provided with grommet type seals 20.At the bottom of the storehouse of settling bin 27, be placed with microslide 33, on microslide 33, be placed with sealing gasket 34, be provided with filter mantle 30 in the upper port of settling bin 27, the bottom of filter mantle 30 is filtering membrane 31, the outer round surface of filter mantle 30 is provided with card step 32, and card step 32 is shelved in the upper port of settling bin 27.
Flaking method of the present invention is specific as follows: first carry out the preparation before film-making, inject appropriate sample reagent to reagent cup 22, on cell settlement film-making working position 9 and DNA film-making working position 10, place respectively settling bin 27, settling bin 27 is fixed on settling bin seat 26, the interior placement microslide 33 of settling bin 27, and filter mantle 30 is placed in the upper port of settling bin 27, sample suction sleeve 23 is socketed in the lower end of sample reagent control end 6.After starting pelleter, suck sample reagent, controller 37 control transverse arm 3 move to reagent draw working position 8 directly over, simultaneously vertical movement arm 4 reagent draw working position 8 directly over after under stretch out sample reagent control end 6, draw the sample reagent in reagent cup 22 in sample reagent liquid sucking device.Then inject sample reagent to sectioning cells: vertical movement arm 4 drives sample reagent control end 6 to move upward to throw off contact, transverse arm 3 move to cell settlement film-making working position 9 directly over, vertical movement arm 4 moves downward until sample reagent control end 6 is positioned at-3 millimeters of places, 2 millimeters of filter mantle 30 tops stops subsequently, and the sample reagent of discharging half is in the settling bin 27 on cell settlement film-making working position 9.Add sample reagent with same principle to DNA film-making: vertical movement arm 4 drives sample reagent control end 6 to move upward, transverse arm 3 move to DNA film-making working position 10 directly over, vertical movement arm 4 moves downward until sample reagent control end 6 is positioned at-3 millimeters of places, 2 millimeters of filter mantle 30 tops stops subsequently, discharges remaining half sample reagent in the settling bin 27 on DNA film-making working position 10.
Slough sample and inhale sleeve, transverse arm 3 and vertical movement arm 4 drive sample reagent control end 6 run to de-cylinder working position 7 directly over, utilize de-frame 35 that sample is inhaled to sleeve 23 and slough from the lower end of sample reagent control end 6.Slough filter mantle: transverse arm 3 and vertical movement arm 4 drive sample reagent control end 6 run to successively cell settlement film-making working position 9 and DNA film-making working position 10 directly over, and sample reagent control end 6 is inserted into respectively to the upper port inside of filter mantle 30, then with filter mantle 30 run to de-cylinder working position 7 directly over, utilize de-frame 35 that filter mantle 30 is sloughed.After until cell, on microslide 33, sedimentation completes, transverse arm 3 and vertical movement arm 4 drive imbibition fluid injection post 5 to run to settling bin 27 tops on DNA film-making working position 10, imbibition fluid injection post 5 blots sample reagent waste liquid only, then with the same manner, the sample reagent waste liquid in settling bin 27 on cell settlement film-making working position 9 is blotted only.
Add ethanol immobile liquid, transverse arm 3 and vertical movement arm 4 drive imbibition fluid injection post 5 to run to respectively settling bin 27 tops on cell settlement film-making working position 9 and DNA film-making working position 10, adding respectively concentration is 90% ethanol immobile liquid, wait for that cell and DNA complete fixing, cell is fixedly in order to strengthen the adhesion of cell on microslide.The ethanol that finally exhausts waste liquid, imbibition fluid injection post 5 runs to respectively settling bin 27 tops on cell settlement film-making working position 9 and DNA film-making working position 10, and by the exhaustion of ethanol waste liquid, sectioning cells and DNA film-making complete respectively, take out sectioning cells and DNA film-making.Can make next column specimen preparation with same circulation step, a row sample can carry out sectioning cells and DNA film-making simultaneously, and film-making process automatically completes, and film-making efficiency is high.
Claims (7)
1. a double-function full automatic cell settlement pelleter, comprise workbench (1), one side of workbench (1) is vertically fixed with column (2), on the upper horizontal guide rail of column (2), transverse arm (3) is installed, the outer end of transverse arm (3) is provided with vertical movement arm (4), it is characterized in that: the upper surface of described workbench (1) is provided with de-cylinder working position (7) near a side of column (2), the opposite side of workbench (1) upper surface is disposed with reagent and draws working position (8), cell settlement film-making working position (9) and DNA film-making working position (10), on de-cylinder working position (7), de-frame (35) is installed, the upper end of de-frame (35) offers bayonet socket (36), reagent is drawn on working position (8) and is provided with sample reagent liquid sucking device, on cell settlement film-making working position (9) and DNA film-making working position (10), lay respectively settling bin seat (26), the upper surface of settling bin seat (26) is provided with arc-shaped slot (28), on settling bin seat (26), be placed with settling bin (27), settling bin (27) is provided with buckle (29) near on the sidewall of bottom corresponding to arc-shaped slot (28), buckle (29) is stuck in the inside of arc-shaped slot (28), the bottom of vertical movement arm (4) is separately installed with imbibition fluid injection post (5) and sample reagent control end (6) by fixed mount.
2. a kind of double-function full automatic cell settlement pelleter according to claim 1, it is characterized in that: described sample reagent liquid sucking device comprises glass stand (21), reagent cup (22), sample is inhaled sleeve (23), step surface (24) and imbibing hole (25), glass stand offers reagent cup putting hole on (21), in reagent cup putting hole, be vertically placed with reagent cup (22), in reagent cup (22), be placed with sample and inhale sleeve (23), sample is inhaled on sleeve (23) upper edge hole and is provided with step surface (24), step surface (24) is shelved in the upper port of reagent cup (22), the bottom that sample is inhaled sleeve (23) offers imbibing hole (25).
3. a kind of double-function full automatic cell settlement pelleter according to claim 1, it is characterized in that: described imbibition fluid injection post (5) is made up of straight tube post (11), imbibition passage (12), pipette (13), reservoir channel (14) and liquid injection pipe (15), in straight tube post (11), offer respectively imbibition passage (12) and reservoir channel (14), imbibition passage (12) is connected with pipette (13), reservoir channel (14) is connected with liquid injection pipe (15), and the length of pipette (13) is greater than the length of liquid injection pipe (15).
4. a kind of double-function full automatic cell settlement pelleter according to claim 1, it is characterized in that: described sample reagent control end (6) is by cylindrical body (16), air flue (17), positive port (18), negative port (19) and grommet type seals (20) form, in cylindrical body (16), offer two parallel air flues (17), the upper port of one of them air flue (17) is positive port (18), the upper port of another air flue (17) is negative port (19), the outer round surface of cylindrical body (16) is provided with grommet type seals (20).
5. a kind of double-function full automatic cell settlement pelleter according to claim 1, it is characterized in that: at the bottom of the storehouse of described settling bin (27), be placed with microslide (33), on microslide (33), be placed with sealing gasket (34), in the upper port of settling bin (27), be provided with filter mantle (30), the bottom of filter mantle (30) is filtering membrane (31), the outer round surface of filter mantle (30) is provided with card step (32), and card step (32) is shelved in the upper port of settling bin (27).
6. a kind of double-function full automatic cell settlement pelleter according to claim 1, is characterized in that: described transverse arm (3) is connected with controller (37) respectively with vertical movement arm (4).
7. a double-function full automatic cell settlement pelleter flaking method, is characterized in that comprising the following steps:
A, film-making are prepared: inject appropriate sample reagent to reagent cup (22), on cell settlement film-making working position (9) and DNA film-making working position (10), place respectively settling bin (27), settling bin (27) is fixed on settling bin seat (26), in settling bin (27), place microslide (33), and filter mantle (30) is placed in the upper port of settling bin (27);
B, suck sample reagent: controller (37) control transverse arm (3) move to reagent draw working position (8) directly over, under while vertical movement arm (4) is backward directly over reagent absorption working position (8), stretch out sample reagent control end (6), draw the sample reagent in reagent cup (22) in sample reagent liquid sucking device;
C, inject sample reagent to sectioning cells: vertical movement arm (4) drives sample reagent control end (6) to move upward to throw off contact, transverse arm (3) move to cell settlement film-making working position (9) directly over, vertical movement arm (4) moves downward until sample reagent control end (6) is positioned at 2 millimeters of-3 millimeters of places, filter mantle (30) top stops subsequently, and the sample reagent of discharging half is in the settling bin (27) on cell settlement film-making working position (9);
D, add sample reagent to DNA film-making: vertical movement arm (4) drives sample reagent control end (6) to move upward, transverse arm (3) move to DNA film-making working position (10) directly over, vertical movement arm (4) moves downward until sample reagent control end (6) is positioned at 2 millimeters of-3 millimeters of places, filter mantle (30) top stops subsequently, discharges remaining half sample reagent in the settling bin (27) on DNA film-making working position (10);
E, slough sample and inhale sleeve: transverse arm (3) and vertical movement arm (4) drive sample reagent control end (6) run to de-cylinder working position (7) directly over, utilize to take off frame (35) and sample is inhaled to sleeve (23) slough from the lower end of sample reagent control end (6);
F, slough filter mantle: transverse arm (3) and vertical movement arm (4) drive sample reagent control end (6) run to successively cell settlement film-making working position (9) and DNA film-making working position (10) directly over, and sample reagent control end (6) is inserted into respectively to the upper port inside of filter mantle (30), then with filter mantle (30) run to de-cylinder working position (7) directly over, utilize de-frame (35) that filter mantle (30) is sloughed;
G, exhaustion sample reagent waste liquid: after until cell, on microslide (33), sedimentation completes, transverse arm (3) and vertical movement arm (4) drive imbibition fluid injection post (5) to run to settling bin (27) top on DNA film-making working position (10), imbibition fluid injection post (5) blots sample reagent waste liquid only, then with the same manner, the sample reagent waste liquid in upper cell settlement film-making working position (9) settling bin (27) is blotted only;
H, add ethanol: transverse arm (3) and vertical movement arm (4) drive imbibition fluid injection post (5) to run to respectively above the settling bin (27) on cell settlement film-making working position (9) and DNA film-making working position (10), add respectively 90% ethanol immobile liquid, wait for that cell and DNA complete fixing;
I, exhaustion ethanol waste liquid: imbibition fluid injection post (5) runs to respectively settling bin (27) top on cell settlement film-making working position (9) and DNA film-making working position (10), ethanol waste liquid is exhausted, sectioning cells and DNA film-making complete respectively, take out sectioning cells and DNA film-making.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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