CN202994806U - Multi-sample sequential loading device for micro-fluidic chip - Google Patents
Multi-sample sequential loading device for micro-fluidic chip Download PDFInfo
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- CN202994806U CN202994806U CN2012206968347U CN201220696834U CN202994806U CN 202994806 U CN202994806 U CN 202994806U CN 2012206968347 U CN2012206968347 U CN 2012206968347U CN 201220696834 U CN201220696834 U CN 201220696834U CN 202994806 U CN202994806 U CN 202994806U
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Abstract
The utility model discloses a multi-sample sequential loading device for a micro-fluidic chip. The device comprises a sample storage tube and a double-way pump, wherein a sample inlet/outlet is formed in one end of the sample storage tube, a sample feeding/discharging control hole is formed in the other end of the sample storage tube, and the double-way pump is communicated with the sample feeding/discharging control hole. A method for carrying out multi-sample sequential loading by utilizing the loading device comprises the steps of: sequentially inhaling a plurality of samples into the sample storage tube by utilizing a negative pressure generated by reverse rotation of the double-way pump according to a sequence opposite to a feeding sequence, and separating the samples by the inhaled isolation gas after inhaling each sample. As the multiple samples are separated by utilizing the air, the sequential loading of the multiple samples can be achieved by utilizing a positive pressure generated by forward rotation of the double-way pump; and compared with the loading device in the prior art, the multi-sample sequential loading device has the advantages that the structure is simple, the manufacture cost is low, the operation is convenient, and the multi-sample sequential loading device is beneficial for popularization and utilization of multi-sample sequential loading and analysis of the micro-fluidic chip.
Description
Technical field
The utility model relates to the chemical analysis technology field, particularly is used for the device that sample loads in a kind of microfluidic chip analysis.
Background technology
Microfluidic chip system is one of the forward position of analysis science and analytical instrument development and most active research field.Along with the develop rapidly of microfluidic chip system, increasing analytical test need to be carried out the Multi-example loading and Multi-example varies, and usually needs to aspirate out the sample in micro-fluidic chip, and loads upper new sample.If thisly vary that to realize varying efficient by manual operations lower, when particularly needing continually Multi-example to be varied, cause the fireballing advantage of chip analysis to can not get demonstrating fully.Therefore, sample loads and varies one of bottleneck that becomes restriction microfluidic chip analysis technical application.
In order to give full play to the advantage of microfluidic analysis technology, and the way in having occurred in prior art the sample that the Sequential Injection Analysis technology is used in microfluidic chip system is loaded and varies (as analytical chemistry, 2003,12:1527-1530).But the Sequential Injection Analysis technology is a kind of liquid handling technology of robotization, the device of realizing Sequential Injection Analysis mainly is comprised of parts such as syringe pump, pressure generating unit, two-way valve, multidigit selector valves, by above-mentioned parts are controlled automatically, can realize the operation to micro liquid (micro updating).This technology is used on microfluidic chip system, more accurately the volume of Quality control, flow velocity and sample loading sequence.But many due to the required hardware component of said apparatus, fluid channel structure is complicated and manufacturing cost is higher, therefore still be difficult to promote and practical.
For above-mentioned deficiency, need to explore and a kind ofly to realize Sequential Injection Analysis and simple in structure, low cost of manufacture, charger easy to use and to realize the method that Multi-example sequentially loads, be difficult to promote and practical problem to solve prior art.
The utility model content
In view of this, the utility model provides a kind of micro-fluidic chip Multi-example order charger, is difficult to promote and practical technical matters to solve existing charger complex structure, high causing of manufacturing cost.
Micro-fluidic chip Multi-example order charger of the present utility model, comprise two-way pump and transparent sample memotron, one end of described sample memotron arranges sample and imports and exports, the other end arranges the sample turnover and controls mouth, described two-way pump is controlled mouth with the sample turnover and is communicated with, and is provided with the minitype gas filtrator between described two-way pump and sample memotron.
Be provided with volume markings on described sample memotron;
Described two-way pump is the adjustable automatic fine syringe pump of injection speed;
Described two-way pump and sample turnover are controlled between mouth and are passed through soft pipe connection.
Described minitype gas filtrator is micropore filtering film.
Described micropore filtering film is 0.22 μ m miillpore filter.
The invention also discloses a kind of described micro-fluidic chip Multi-example order charger that utilizes and carry out the method that sample sequentially loads, comprise the following steps:
A) sampling: by negative pressure, several samples is sucked the sample storage pipe successively by the reverse order of sample introduction order, suck separation gas after a kind of sample of every suction and isolate, form the storehouse of multistage sample in the sample memotron;
B) load: the sample of sample storage pipe is imported and exported be communicated with the injection port of micro-fluidic chip, complete the sample loading by the micro-fluidic chip of flowing through successively of the multistage sample in malleation Quality control storage tube.
Negative pressure and the described step b of described step in a)) malleation in is produced by two-way pump;
The separation gas of described step in a) is clean air.
the beneficial effects of the utility model: micro-fluidic chip Multi-example order charger of the present utility model, comprise sample memotron and two-way pump, one end of described sample memotron arranges sample and imports and exports, the other end arranges the sample turnover and controls mouth, described two-way pump is controlled mouth with the sample turnover and is communicated with, utilize above-mentioned charger to carry out the method that Multi-example sequentially loads to be: by negative pressure, several samples is sucked the sample storage pipe successively by the reverse order of sample introduction order, sucking separation gas after a kind of sample of every suction isolates, owing to adopting air to carry out isolation between Multi-example, the order that can realize Multi-example by malleation loads, compare with charger of the prior art, simple in structure, low cost of manufacture, easy to operate, be conducive to Multi-example and sequentially load the popularization of analysis and practical.
Description of drawings
Below in conjunction with drawings and Examples, the utility model is further described.
Fig. 1 is structural representation of the present utility model;
Fig. 2 utilizes charger of the present utility model to carry out the schematic flow sheet that Multi-example sequentially loads.
Embodiment
Below with reference to accompanying drawing, the utility model is elaborated, as shown in the figure: the micro-fluidic chip Multi-example order charger of the present embodiment, comprise two-way pump 2 and transparent sample memotron 1, transparent sample storage pipe is convenient to observe inlet and the present position of sample, one end of described sample memotron 1 arranges sample and imports and exports 1a, the other end arranges the sample turnover and controls a mouthful 1b, described two-way pump 2 is controlled a mouthful 1b with the sample turnover and is communicated with, so that to applying negative pressure or malleation, the turnover of Quality control in the sample storage pipe.It should be noted that the diameter of sample storage pipe is unsuitable excessive, so that the surface tension of liquid can keep liquid stabilising to adhere in order to make liquid enter stable being attached on tube wall of sample storage Guan Houneng.Be provided with minitype gas filtrator 10 between described two-way pump 2 and sample memotron 1, described minitype gas filtrator 10 is 0.22 μ m micropore filtering film, to guarantee that sample is not by the contaminants in gas.
As improvement, be provided with volume markings 1c on described sample memotron 1, accurate Quality control inlet satisfies the needs of microfluidic chip analysis.
Preferably, described two-way pump 2 is the adjustable automatic fine syringe pump of injection speed, by automated mechanical device pushing syringe, can realize that high precision, steady pulseless sample load, and can press the certain speed load sample according to the requirement of chip analysis.
Preferably, described two-way pump 2 is controlled between mouthful 1b with the sample turnover and is communicated with by flexible pipe 3, and when easy to use, two-way pump is arbitrarily put.
A kind of described micro-fluidic chip Multi-example order charger that utilizes carries out the method that Multi-example sequentially loads, and comprises the following steps:
A) sampling: produce negative pressure by two-way pump 2 several samples is sucked sample storage pipe 1 successively by the reverse order of sample introduction order, suck separation gas 4 after a kind of sample of every suction and isolate, form the storehouse of multistage sample in sample memotron 1;
As shown in Figure 2, the present embodiment needs that four kinds of samples are carried out order and loads: sample I6, sample II7, sample III8 and sample IV9 carry out order and load, the reverse order of pressing loading sequence sample I6, sample II7, sample III8 and sample IV9 sucks sample, suck one section separation gas 4 after a kind of sample of every suction and carry out the interval, form at last the storehouse that sample I6, sample II7, sample III8 and sample IV9 are arranged in order from the bottom to top in the sample storage pipe.
B) load: the sample of sample storage pipe 1 is imported and exported 1a be communicated with the injection port of micro-fluidic chip 5, according to analyzing required loading velocity, produces multistage sample in malleation Quality control storage tube 1 micro-fluidic chip 5 of flowing through successively by two-way pump and complete the sample loading.
The utility model is realized the isolation of sample room by gas, avoid using structure and control complicated order loading analytical equipment, effectively reduces and analyzes required cost, and is convenient and practical, is suitable for large application scope.
Preferably, the separation gas 4 of described step in a) is clean air, and be easy to operate, cost is lower, certainly, if sample should not contact with air, inert gas or other gas that does not affect sample that can select to suck cleaning carry out the isolation of sample room, all can realize the purpose of this utility model.
Explanation is at last, above embodiment is only unrestricted in order to the technical solution of the utility model to be described, although with reference to preferred embodiment, the utility model is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement the technical solution of the utility model, and not breaking away from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of claim scope of the present utility model.
Claims (6)
1. micro-fluidic chip Multi-example order charger, it is characterized in that: comprise two-way pump (2) and transparent sample memotron (1), one end of described sample memotron (1) arranges sample and imports and exports (1a), the other end arranges the sample turnover and controls mouthful (1b), described two-way pump (2) is controlled mouthful (1b) with the sample turnover and is communicated with, and is provided with minitype gas filtrator (10) between described two-way pump (2) and sample memotron (1).
2. micro-fluidic chip Multi-example order charger according to claim 1, is characterized in that: be provided with volume markings (1c) on described sample memotron (1).
3. micro-fluidic chip Multi-example according to claim 2 order charger, it is characterized in that: described two-way pump (2) is the adjustable automatic fine syringe pump of injection speed.
4. micro-fluidic chip Multi-example according to claim 3 order charger, it is characterized in that: described two-way pump (2) is controlled between mouth (1b) with the sample turnover and is communicated with by flexible pipe (3).
5. micro-fluidic chip Multi-example according to claim 4 order charger, it is characterized in that: described minitype gas filtrator (10) is micropore filtering film.
6. micro-fluidic chip Multi-example according to claim 5 order charger, it is characterized in that: described micropore filtering film is 0.22 μ m miillpore filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012206968347U CN202994806U (en) | 2011-12-27 | 2012-12-17 | Multi-sample sequential loading device for micro-fluidic chip |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120555810 | 2011-12-27 | ||
| CN201120555810.5 | 2011-12-27 | ||
| CN2012206968347U CN202994806U (en) | 2011-12-27 | 2012-12-17 | Multi-sample sequential loading device for micro-fluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202994806U true CN202994806U (en) | 2013-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012206968347U Expired - Fee Related CN202994806U (en) | 2011-12-27 | 2012-12-17 | Multi-sample sequential loading device for micro-fluidic chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872642A (en) * | 2017-01-03 | 2017-06-20 | 北京雪迪龙科技股份有限公司 | A kind of micro-sampling pipe |
| CN106984370A (en) * | 2017-05-08 | 2017-07-28 | 北京旌准医疗科技有限公司 | A kind of automatic sample handling system based on micro-fluidic chip |
-
2012
- 2012-12-17 CN CN2012206968347U patent/CN202994806U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106872642A (en) * | 2017-01-03 | 2017-06-20 | 北京雪迪龙科技股份有限公司 | A kind of micro-sampling pipe |
| CN106872642B (en) * | 2017-01-03 | 2020-01-17 | 北京雪迪龙科技股份有限公司 | Trace sampling tube |
| CN106984370A (en) * | 2017-05-08 | 2017-07-28 | 北京旌准医疗科技有限公司 | A kind of automatic sample handling system based on micro-fluidic chip |
| CN106984370B (en) * | 2017-05-08 | 2019-09-20 | 北京旌准医疗科技有限公司 | A kind of automatic sample handling system based on micro-fluidic chip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130612 Termination date: 20131217 |