TWM627564U - Closed Cell Separation Operation Device - Google Patents

Abstract

The present invention creates a closed cell separation operation device, which includes: a first tube body with a first opening, a first bottom and a first cover body, the first cover body is provided with a first air hole and an input hole , the first bottom is provided with an output hole; and a second pipe body has a second opening, a second bottom and a second cover body, the second cover body is provided with a second air hole and a connecting hole, the connecting hole It is connected with the output hole by a connecting tube; after the cell sample placed in the first tube body is centrifuged to produce layers, the cell samples of different layers can be extracted from the first tube body to the second tube body by means of air pressure and other means. Two tubes; thus, this creation can form a closed environment, and the process of inserting cell samples, centrifugation and separation are all carried out in it, which can effectively prevent the pollution problem during the operation.

Description

Closed Cell Separation Operation Device

This creation is related to the cell separation operation device, and more specifically, it refers to a cell separation operation device with good sealing performance, which can effectively prevent the pollution problem during the operation. The cell sample can be peripheral blood, bone marrow aspirate, spinal cord aspirate, ascites aspirate, urine, cell culture fluid, and other liquids containing cells.

The conventional cell separation device (such as for blood centrifugation) needs to first extract the blood stored in the blood bag and the test tube, and transfer it into a centrifuge tube by means of a needle tube, cover it, and then put it in the centrifuge. Centrifuge in the machine. After the blood is centrifuged, it will be separated into two parts: serum and blood cells. The upper layer contains platelets and growth factors, and the lower layer contains all blood cells. After opening the cover, the upper or lower layer of liquid is extracted with a needle tube according to the needs of the inspection for subsequent inspection.

At present, most of the existing centrifuge test tubes on the market are hollow tubes with a screw cap on the opening. The cap needs to be unscrewed so that blood can be put in before the cap is tightened. After centrifugation, the cap needs to be unscrewed. The upper and lower layers of liquid are sucked out for operation. During the operation, the cover needs to be opened continuously in the open space. For clinical use, not only the operation steps are cumbersome, but also the process needs to be carefully operated or it will be easily contaminated. Unless it can be operated in a sterile room or used in conjunction with a large negative pressure extraction console, it is difficult to eliminate the concern of contamination. However, the installation cost of the sterile room and the extraction console is high, and the use site has its limitations.

In order to solve the above-mentioned problems, the present invention discloses a closed cell separation operation device, which can provide a closed operation environment, and the processes from inserting cell samples, centrifugation and separation are all carried out in the closed environment, which can effectively prevent the pollution during the operation process. In addition, there is no need to cooperate with equipment such as a sterile room and an air extraction console, so as to achieve the purpose of providing an excellent, simple and low-cost operating device.

In order to achieve the above-mentioned purpose, the present invention provides a closed cell separation operation device in one embodiment, which includes:

A first tube body, which is generally in the shape of a hollow tube body. The first tube body has a first opening and a first bottom. A sealable first cover is provided at the first opening. The first cover A first air hole and an input hole are arranged on the top, an output hole is arranged at the first bottom; and a second pipe body is generally in the shape of a hollow pipe body, and the second pipe body has a second opening and a second The bottom, the second opening is provided with a sealable second cover body, the second cover body is provided with a second air hole and a connecting hole, the connecting hole and the output hole are connected by a connecting pipe. In this way, containers such as blood bags, test tubes, and needle tubes containing cell samples are connected to the input tube. After the cell samples are input into the inside of the first tube body, the first tube body and the second tube body are placed together in the input tube. Centrifugation in a centrifuge allows the cell sample to be layered in the first tube, and then at least a part of the layered cell sample is separated into the second tube through a connecting tube.

In one embodiment, an air pressure source is further included, and the air pressure source can be movably connected to at least one of the first air hole or the second air hole, and the air pressure source can be a syringe, an air pump, an air pump or an air pump. Pu one of them. The positive pressure or negative pressure generated by the air pressure source can facilitate the layer separation of the cell sample from the first tube body to the second tube body.

In one embodiment, an expansion tube disposed in the expansion hole is further included, and the expansion tube can be connected to another set of the present invention, so as to facilitate the operation of multiple centrifugal and stratified separation.

In one embodiment, a first foot seat extends downward from the outer periphery of the first bottom of the first tube body, the first foot seat and the first bottom are surrounded to form a first storage space, and the first storage space can be used for At least a part of the connecting pipe is accommodated. The outer periphery of the second bottom of the second tube body extends downward with a second foot seat, the second foot seat and the second bottom are surrounded to form a second storage space, and the first storage space can accommodate the expansion tube. It is convenient for the first tube body and the second tube body to stand when they are placed in the centrifuge, or placed on the table top.

In order to facilitate the description of the central idea expressed in the above-mentioned column of creative content of this creation, specific embodiments are used to express it. Various objects in the embodiments are drawn according to the scale suitable for enumeration and description, rather than the scale of the actual elements, and will be described together first.

Please refer to FIG. 1 to FIG. 6 , the present invention is a closed cell separation operation device, which includes:

A first tube body 10 is generally in the shape of a transparent hollow tube body, the first tube body 10 has a first opening 11 and a first bottom 12 , and a sealable first cover is arranged at the first opening 11 The body 13, the first cover body 13 is provided with a first air hole 131 and an input hole 132, the first bottom 12 is provided with an output hole 121, wherein the first bottom 12 is a conical concave bottom, and the output hole 121 is provided with At the lowest position of the conical concave bottom; furthermore, a first foot seat 122 extends downward from the outer periphery of the first bottom portion 12 of the first tube body 10 . The first foot seat 122 and the first bottom portion 12 are surrounded to form a first foot seat 122 A storage space 123 is provided, and at least one first gap 124 is defined on the first foot base 122 .

A second tube body 20 is generally in the shape of a transparent hollow tube body, the second tube body 20 has a second opening 21 and a second bottom 22 , and a sealable second cover is arranged at the second opening 21 The body 23, the second cover body 23 is provided with a second air hole 231 and a connecting hole 232, the connecting hole 232 and the output hole 121 are connected by a connecting pipe 24. Furthermore, the second bottom portion 22 is provided with an expansion hole 221 . Wherein, the second bottom 22 is a conical concave bottom, and the expansion hole 221 is arranged at the lowest part of the conical concave bottom. A second base 222 extends downward from the outer periphery of the second bottom 22 of the second tube body 20 . The second base 222 and the second bottom 22 surround a second storage space 223 . At least one second gap 224 is opened.

One end of a first air pipe 30 is disposed at the first air hole 131 , and the other end of the first air pipe 30 is connected with a first filter 31 .

An input tube 40, one end of which is disposed at the input hole 132, and the other end of the input tube 40 can be connected to containers such as blood bags, test tubes, and needle tubes containing cell samples, so as to facilitate the input of cell samples into the first tube inside the body 10 .

An expansion tube 50 has one end disposed at the expansion hole 221 .

Furthermore, at least one valve body 60 is provided on the input pipe 40 , the connecting pipe 24 , the first air pipe 30 and the expansion pipe 50 , and the valve body 60 is at least one of a pipe clamp, a flow valve or an on-off valve. It is used as a control for closing or opening of the above-mentioned pipes.

As shown in FIG. 3 , the present invention further includes an air pressure source 70 . In this embodiment, the air pressure source 70 is movably connected to the second air hole 231 , wherein the air pressure source 70 can be a syringe, an air pump, and an air pump. Or pump one of them.

Furthermore, the present invention further includes at least one closure member 80 , and the closure member 80 can be movably closed on at least one of the first air hole 131 , the input hole 132 , the output hole 121 , the second air hole 231 , the connecting hole 232 and the expansion hole 221 . one. Alternatively, the closing element 80 can also be movably closed at the other end of at least one of the input pipe 40 , the connecting pipe 24 , the first trachea 30 and the expansion pipe 50 . According to the needs of use, it can be closed in the above places to prevent the contamination caused by the entry of foreign objects. For example, in a possible embodiment, the closure member 80 can be closed at the second air hole 231 first, and the air pressure source 70 needs to be flexibly connected. Take it off, and after the air pressure source 70 is used up, it is closed again in the second air hole 231 .

Please refer to FIG. 3 , when the present invention is implemented, a blood bag, a test tube or a needle tube containing a cell sample can be connected to the input tube 40 , and the valve body 60 on the input tube 40 and the first trachea 30 can be opened to facilitate the Input the cell sample into the first tube body 10 , and then put the first tube body 10 and the second tube body 20 together in a centrifuge for centrifugation, so as to facilitate the generation of the cell sample in the first tube body 10 Layered. Furthermore, when the first tube body 10 and the second tube body 20 are placed in the centrifuge, at least a part of the connecting tube 24 can be rolled up and stored in the first storage space 123, and the expansion tube 50 can be rolled up and stored in the centrifuge. In the second storage space 223 , the first tube body 10 and the second tube body 20 can be placed side by side in the centrifuge to avoid interference between the connecting tube 24 and the expansion tube 50 during centrifugation.

Please refer to FIG. 4 , the cell sample in the first tube body 10 will be stratified after centrifugation. For example, when the cell sample is blood, it will be separated into two parts: serum and blood cells. The upper layer is the serum containing platelets and the blood cells. Growth factors and other substances, and the lower layer contains all the blood cells. At this time, the air pressure source 70 is movably connected to the second air hole 231, and the valve body 60 on the connecting pipe 24 and the first air pipe 30 is opened, so as to use the negative pressure air pressure method to separate the centrifuged and stratified cell samples from the first air pipe. The inside of the pipe body 10 is extracted and transported into the second pipe body 20 through the connecting pipe 24 .

Since the first bottom 12 is a conical concave bottom, and the output hole 121 is located at the lowest part of the conical concave bottom, the user can precisely control the separation of the upper and lower layers when taking the cell sample from the lower layer to the second tube body 20 . For the cell sample, as shown in FIG. 4 , the upper cell sample is kept in the first tube body 10 , and the lower cell sample is stored in the second tube body 20 . At this time, each valve body 60 is closed or further closed by the closure member 80 . , the first tube body 10 and the second tube body 20 can be separated, so as to facilitate individual inspection or processing. Furthermore, the air entering and exiting the first pipe body 10 during the above operation is processed by the first filter 31, which can effectively prevent the entry of foreign matter or pollution.

Furthermore, as shown in FIG. 5 , the expansion tube 50 of the second tube body 20 can be connected to another set of the input holes 132 of the first tube body 10 of the present invention, so as to facilitate the process of multiple centrifugal and stratified separation. operate.

In addition, the first leg 122 and the second leg 222 can accommodate the connecting tube 24 and the expansion tube 50, and the first gap 124 and the second gap 224 can be used for the connecting tube 24 and the expansion tube 50 to be arranged around the connection tube 24 and the expansion tube 50. It is convenient for the user to place the first tube body 10 and the second tube body 20 on a desktop or the like for standing.

As shown in FIG. 6 , which is another embodiment of the present invention, which further includes a second air pipe 90 disposed in the second air hole 231 , and at least one valve body 60 is provided on the second air pipe 90 . The valve body 60 It is at least one of a pipe clamp, a flow valve or an on-off valve. The second air pipe 90 is connected with a second filter 91 , and the closing element 80 can also be movably closed at the other end of the second air pipe 90 . Furthermore, in this embodiment, the air pressure source 70 is movably connected to the second air hole 231 , so that the cell sample after centrifugation and stratification is pushed from the first tube body 10 through the connecting tube 24 by means of positive air pressure. into the second pipe body 20 . In the above operation process, the air entering and exiting the second pipe body 20 is processed by the second filter 91, which can effectively prevent the entry of foreign matter or pollution.

In the present invention, the first tube body 10 and the second tube body 20 are used to form a small and closed operating system relative to the sterile room and the pumping console, and the cell samples are input, centrifuged, and separated from the upper and lower layers and stored in different tubes. There is no need to open the cover body, effectively preventing foreign matter or pollution from entering during operation. These filters also ensure the quality of incoming and outgoing air, enhancing the effect of blocking pollution. In addition, this creation does not require the cooperation of equipment such as a sterile room and an air extraction console, which not only has an excellent isolation effect, is easy to use, and has a low cost, but can also be used as a one-time-use operating device.

Although the present invention is described with several preferred embodiments, those skilled in the art can make various changes without departing from the spirit and scope of the present invention. The above-mentioned embodiments are only used to illustrate the present creation, and are not intended to limit the scope of the present creation. All kinds of modifications or changes that do not violate the spirit of this creation belong to the scope of the patent application for this creation.

10: The first tube body 11: The first opening 12: First Bottom 121: output hole 122: The first foot seat 123: The first storage space 124: First Gap 13: The first cover 131: The first air hole 132: Input hole 20: Second tube body 21: Second opening 22: Second Bottom 221: Expansion hole 222: Second foot seat 223: Second storage space 224: Second Gap 23: Second cover 231: Second air hole 232: Connection hole 24: connecting pipe 30: First trachea 31: First Filter 40: Input tube 50: Expansion tube 60: valve body 70: Air pressure source 80: Closure 90: Second trachea 91: Second filter

[FIG. 1] is a perspective view of the first embodiment of the present invention. [Figure 2] is a schematic diagram of the movement of this creation (1). [Figure 3] is a schematic diagram of the action of this creation (2). [Figure 4] is a schematic diagram of the action of this creation (3). [Figure 5] is a schematic diagram of the use of multiple sets of this creation connection. [FIG. 6] is a schematic diagram of another embodiment of the present invention.

10: The first tube body

122: The first foot seat

124: First Gap

13: The first cover

20: Second tube body

222: Second foot seat

224: Second Gap

23: Second cover

24: connecting pipe

30: First trachea

31: First Filter

40: Input tube

50: Expansion tube

60: valve body

80: Closure

Claims (19)

A closed cell separation operation device, comprising: A first tube body, which is generally in the shape of a hollow tube body, the first tube body has a first opening and a first bottom, the first opening is provided with a sealable first cover, the first A cover body is provided with a first air hole and an input hole, and the first bottom is provided with an output hole; and A second tube body, which is generally in the shape of a hollow tube body, the second tube body has a second opening and a second bottom, a second sealable cover is arranged at the second opening, and the first A second air hole and a connecting hole are arranged on the two cover bodies, and a connecting pipe is connected between the connecting hole and the output hole. The closed cell separation operation device according to claim 1, further comprising an air pressure source, which is movably connected to at least one of the first air hole or the second air hole. The closed cell separation operation device according to claim 2, wherein the air pressure source can be one of a syringe, an air pump, an air pump or an air pump. The closed cell separation operation device as claimed in claim 1, wherein the second bottom is provided with an expansion hole. The closed cell separation operation device according to claim 4, further comprising an expansion tube disposed in the expansion hole. The closed cell separation operation device according to claim 5, further comprising a first trachea disposed in the first air hole. The closed cell separation operation device according to claim 6, further comprising an input tube disposed in the input hole. The closed cell separation operation device according to claim 7, further comprising a second trachea disposed in the second air hole. The closed cell separation operation device according to claim 8, wherein at least one valve body is provided on the input pipe, the connecting pipe, the first trachea, the second trachea and the expansion pipe. The closed cell separation operation device according to claim 9, wherein the valve system is at least one of a pipe clamp, a flow valve or an on-off valve. The closed cell separation operation device as claimed in claim 8, further comprising at least one closure member, which can be movably closed on the input tube, the connecting tube, the first trachea, the second trachea at the other end of at least one of the trachea and the expansion tube. The closed cell separation operation device according to claim 4, further comprising at least one closing element, which can be movably closed in the first air hole, the input hole, the output hole, the second air hole, and the second air hole. at least one of the air hole, the connection hole and the expansion hole. The closed cell separation operation device as claimed in claim 4, wherein the first bottom and the second bottom are a conical concave bottom, and the output hole and the expansion hole are arranged at the lowest point of the conical concave bottom place. The closed cell separation operation device according to claim 6, wherein the first trachea is provided with a first filter. The closed cell separation operation device according to claim 8, wherein the second trachea is provided with a second filter. The closed cell separation operation device as claimed in claim 1, wherein the outer periphery of the first bottom of the first tube body extends downward with a first foot seat, and the first foot seat and the first bottom form a surrounding area. A first storage space. The closed cell separation operation device as claimed in claim 16, wherein at least one first gap is formed on the first foot. The closed cell separation operation device as claimed in claim 1, wherein the outer periphery of the second bottom of the second tube body extends downward with a second foot seat, and the second foot seat and the second bottom are formed around the second foot. A second storage space. The closed cell separation operation device as claimed in claim 18, wherein at least one second gap is formed on the second base.

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