CN206411133U - A kind of loading pipe for flow cytometer - Google Patents
A kind of loading pipe for flow cytometer Download PDFInfo
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- CN206411133U CN206411133U CN201621243660.3U CN201621243660U CN206411133U CN 206411133 U CN206411133 U CN 206411133U CN 201621243660 U CN201621243660 U CN 201621243660U CN 206411133 U CN206411133 U CN 206411133U
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- Prior art keywords
- loading pipe
- flow cytometer
- pipe
- filter
- closure
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- 238000007789 sealing Methods 0.000 claims abstract description 4
- 230000007306 turnover Effects 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 abstract description 12
- 230000001954 sterilising effect Effects 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 15
- 238000001914 filtration Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 230000002776 aggregation Effects 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000013536 elastomeric material Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a kind of loading pipe for flow cytometer, including the first body, filter is provided with first body at the mouth of pipe, filter includes the filter screen of second body one end of the second body and covering of both ends open, and along its edge, outwards turnover has flange to the other end of the second body；The inwall of first body is formed with its mouth of pipe can support the first step of flange；Also include sealing the closure for covering the mouth of pipe on the first body, the outer wall of the first body, which is provided with, can make the second step that loading pipe lies on the table.The loading pipe is easy to operate, it is to avoid sample is lost in filter process, and convenient progress sterilization treatment.
Description
Technical field
The utility model is related to a kind of loading pipe for flow cytometer.
Background technology
Flow cytometer is a kind of collection laser technology, electronics physical technique, photoelectric measurement technology, electronic computer and thin
The new high-tech instrument that born of the same parents' fluorescence chemical technology, monoclonal antibody technique are integrated, mainly by liquid fluid system, optical system,
Electronic system, 5 parts of data handling system and cell sorting system are constituted.In short, flow cytometry is exactly for place
Individual cells or biologic grain in quick straight line flow regime carry out multi-parameter, quick quantitative analysis and the skill of sorting
Art.So flow cytometer needs to filter out cell mass or big particle before detection sample, it is ensured that the accuracy of detection, together
When avoid instrument liquid fluid system pipeline blockage.
Flow cytometer carry out various parameters analysis quick to cell must be based on single cell basis, so must before sample introduction
Contain a large amount of fragments of tissue inside palpus cell dispersion, the sample of especially various tissue digestions.Needed before detection sample to sample
This suspension is filtered, to filter out large crumb, if do not filtered out, and can be blocked instrument internal pipeline in inspection detection process, be caused
Instrument failure；Some dyestuffs can also cause cell aggregation, and the PI dyestuffs that for example the detection cell cycle uses can cause cell aggregation,
Filtering sample must be used in the detection cell cycle；In addition, when doing some material toxicity tests, material can be adsorbed in cell table
Face, causes cell aggregation, is so also required to filtering.
The filter method filtered currently used for flow cytometer sample is that a bulk of filter screen is cut into some fritters, Ran Houqu
One fritter gets lodged in the loading mouth of pipe, then sample note is entered against fritter filter screen with pipettor.This filter method is excessively coarse, mistake
Filter is not clean；Inconvenient operation, easily causes cross pollution between sample；And easily cause sample loss, influence experiment detection
As a result；It cannot sterilize for aseptic experiment.
In view of above-mentioned defect, the design people is actively subject to research and innovation, a kind of for flow cytometer to found
Loading pipe, make it with more the value in industry.
Utility model content
In order to solve the above technical problems, the purpose of this utility model is to provide a kind of loading pipe for flow cytometer,
The loading pipe is easy to operate, it is to avoid sample is lost in filter process, and facilitates sterilization treatment.
Close to pipe in loading pipe of the present utility model for flow cytometer, including the first body, first body
Filter is provided with mouthful, the filter includes the filter screen of second body one end of the second body and covering of both ends open,
Along its edge, outwards turnover has flange to the other end of second body；The inwall of first body is formed at its mouth of pipe
There is the first step that can support the flange.
Further, in addition to can closure residence state the closure of the mouth of pipe on the first body.
Further, the outer wall of first body, which is provided with, can make the second step that the loading pipe lies on the table.
Further, the section of the closure is spill.
Further, one or more of first body, the second body, filter screen, closure are by high temperature high voltage resistant
Material is made, such as polystyrene, polypropylene or polythene material.
Further, the closure is made up of elastomeric material.
By such scheme, the utility model at least has advantages below：
1st, it is equipped with a kind of loading pipe for flow cytometer of the present utility model, the first body by first step pavilion
The second body with filter screen, it is easy to operate, it is to avoid sample is lost in filter process, sample is saved, experimental cost is reduced；Sample
After handling well, loading pipe is directly added into, the rotor hole of body size and centrifuge matches, and can be used on centrifuge, lead to
Cross low-speed centrifugal cause filtering faster, it is more thorough；
2nd, the mouth of pipe of the first body is provided with closure, it is to avoid loading pipe liquid in centrifugation spills the first body, and close
The section of capping is concave, it is to avoid the sample of filtering is polluted by closure；
3rd, the first body is externally provided with second step, facilitates loading pipe to shelve and is fixed on flow cytometer；
4th, the first body, the second body, filter screen, closure are made up of the material of high temperature high voltage resistant, may be sterilized processing,
It can be used in needing the cell sorting of sterile working to test, the cell after sorting can be used for the later stage to expand culture, existing streaming
Cell filtration sport technique segment is more, it is easy to contaminated, especially sorts the stem cell in some tissues, due to tissue digestion not
Fully, it is necessary to filter, and use traditional filter method, sample is easy to be contaminated, the loading pipe can with autoclave sterilization,
It is easy to use, many steps are saved, sample are reduced because the contaminated possibility of misoperation；
5th, closure soft material is made, and is further ensured that sealing；
6th, loading pipe of the present utility model, is adapted to handle high-volume sample simultaneously, saves the time, improves conventional efficient, and
And the cross pollution in filter process of different samples can be avoided；Furthermore it is possible to be made marks on pipe, it is to avoid in filter process
Mistake, which is taken by mistake, puts caused error；Discarded pipe is easily collected, it is to avoid the filter screen fragment pollution experiment in conventional filtering method
The problem of room environmental.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.
Brief description of the drawings
Fig. 1 is the structural representation of loading pipe in the utility model；
Fig. 2 is the decomposition texture schematic diagram of loading pipe in the utility model.
Embodiment
With reference to the accompanying drawings and examples, embodiment of the present utility model is described in further detail.Below
Embodiment is used to illustrate the utility model, but is not limited to scope of the present utility model.
Referring to Fig. 1 and Fig. 2, a kind of loading pipe for flow cytometer described in the preferred embodiment of the utility model one,
The loading pipe of the present invention includes being provided with filter in the first body 1, the first body 1 at the mouth of pipe, and filter includes two
Second body 2 of end opening and the filter screen 3 of covering the second body 2 one end, the other end of the second body 2 are outwards turned down along its edge
There is flange 4；The inwall of first body 1 is formed with its mouth of pipe can support the first step 5 of flange 4；Loading pipe is also wrapped
The closure 6 for covering the mouth of pipe on the first body 1 can be sealed by including, and closure 6 is made up of elastomeric material, and its section is recessed
Shape；Be made up in first body 1, the second pipe 2, filter screen 3, closure 6 of high pressure resistant high temperature material, such as polystyrene, polypropylene or
Polythene material, it is convenient to carry out sterilization treatment；The outer wall of first body 1 is provided with second that the loading pipe can be made to lie on the table
Step 7, facilitates its Kato to be detected on flow cytometer.
Loading pipe can integrally be divided into three parts：(1) first body 1；(2) filter；(3) closure 6, this three part can
Used with being connected as a single entity, can also dismantle and be independently operated, three parts by can be made with the material of autoclave sterilization, its
In, the size of the first body 1 and conventional centrifugal basket sub-aperture match, and can centrifuge, not only can be for filtration cell, also
Other materials can be filtered, it is quite varied using scope for other experiments；Closure 6 is made up of soft rubber material, more protects
Its sealing effectiveness is demonstrate,proved；The aperture of filter screen 3 can be designed as 40um, 70um, 100um, and user can select according to requirement of experiment
Loading pipe with different pore size filter screen 3, filters different size of cell and particle；Outwards turnover has the upper end-face edge of second body 2
Can flange 4 of the Kato on first step 5, can be good at fixing filter screen 3.
Loading pipe of the present utility model is as follows using step：
The second body 2 for having suitable aperture (40um, 70um, 100um) filter screen 3 is selected to be put into the first body 1 as needed
In, if desired aseptic condition, autoclave sterilization is first carried out to loading pipe；Then suspension to be filtered is moved into filter using pipettor
On net 3, and sealed with closure 6；Then loading pipe is put into centrifuge, low-speed centrifugal filtering；After centrifugal filtration, taken with tweezers
Go out filter, machine testing on loading pipe is obtained into data；Experiment terminate after by three parts of loading pipe dismantle and cleaned,
Sterilizing, is reused.
Described above is only preferred embodiment of the present utility model, is not limited to the utility model, it is noted that
For those skilled in the art, on the premise of the utility model technical principle is not departed from, it can also do
Go out some improvement and modification, these improvement and modification also should be regarded as protection domain of the present utility model.
Claims (6)
1. a kind of loading pipe for flow cytometer, it is characterised in that：It is close in first body to manage including the first body
Filter is provided with mouthful, the filter includes the filter screen of second body one end of the second body and covering of both ends open,
Along its edge, outwards turnover has flange to the other end of second body；The inwall of first body is formed at its mouth of pipe
There is the first step that can support the flange.
2. the loading pipe according to claim 1 for flow cytometer, it is characterised in that：Also include sealing covering
The closure of the mouth of pipe on first body.
3. the loading pipe according to claim 1 or 2 for flow cytometer, it is characterised in that：First body
Outer wall, which is provided with, can make the second step that the loading pipe lies on the table.
4. the loading pipe according to claim 2 for flow cytometer, it is characterised in that：The section of the closure is
Spill.
5. the loading pipe according to claim 1 for flow cytometer, it is characterised in that：First body, second
One or more of body, filter screen, closure are made up of high temperature high voltage resistant material.
6. the loading pipe according to claim 5 for flow cytometer, it is characterised in that：The closure is by rubber material
Material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621243660.3U CN206411133U (en) | 2016-11-16 | 2016-11-16 | A kind of loading pipe for flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621243660.3U CN206411133U (en) | 2016-11-16 | 2016-11-16 | A kind of loading pipe for flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206411133U true CN206411133U (en) | 2017-08-15 |
Family
ID=59548411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621243660.3U Active CN206411133U (en) | 2016-11-16 | 2016-11-16 | A kind of loading pipe for flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206411133U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| CN108721964A (en) * | 2017-12-15 | 2018-11-02 | 山东省医学科学院基础医学研究所 | A kind of preparation method of inserted streaming filter membrane |
| CN111085027A (en) * | 2019-12-06 | 2020-05-01 | 南通大学 | Reusable flow cytometry sample filtering device |
-
2016
- 2016-11-16 CN CN201621243660.3U patent/CN206411133U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721964A (en) * | 2017-12-15 | 2018-11-02 | 山东省医学科学院基础医学研究所 | A kind of preparation method of inserted streaming filter membrane |
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| CN108344697B (en) * | 2018-05-14 | 2024-02-09 | 苏州大学 | Special sample loading frame combination for in-vitro samples of photoacoustic tomography molecular imaging system |
| CN111085027A (en) * | 2019-12-06 | 2020-05-01 | 南通大学 | Reusable flow cytometry sample filtering device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200917 Address after: Room 203, building 14, Xibei District, Suzhou nano City, No. 99, Jinjihu Avenue, Suzhou Industrial Park, Suzhou, Jiangsu Province Patentee after: Suzhou boaolong Technology Co., Ltd Address before: 215000 Suzhou Industrial Park, Jiangsu Road, No. 199 Patentee before: Suzhou University |
|
| TR01 | Transfer of patent right |