CN206751794U - A kind of enzymolysis liquid filter for being used to prepare plant protoplast - Google Patents
A kind of enzymolysis liquid filter for being used to prepare plant protoplast Download PDFInfo
- Publication number
- CN206751794U CN206751794U CN201720525722.8U CN201720525722U CN206751794U CN 206751794 U CN206751794 U CN 206751794U CN 201720525722 U CN201720525722 U CN 201720525722U CN 206751794 U CN206751794 U CN 206751794U
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- CN
- China
- Prior art keywords
- enzymolysis liquid
- sleeve
- plant protoplast
- funnel
- small opening
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of enzymolysis liquid filter for being used to prepare plant protoplast, including funnel, the bottom of the funnel is provided with small opening pipeline, detachable nylon net filter film is provided with the inwall of the funnel, hollow sleeve is connected with below the small opening pipeline, coupled logical injection head is provided with the top of the sleeve, the small opening pipeline is inserted in injection head；Piston rod is provided with the sleeve, the other end of the piston rod is stretched out outside the bottom of sleeve, and lug is provided with the bottom of the sleeve outer wall.The present apparatus can prevent the loss that enzymolysis liquid is spilt.The suction filtration to enzymolysis liquid is realized, makes thick and sticking enzymolysis liquid be easy to pass through nylon net filter film, makes experimental implementation enhanced convenience.
Description
Technical field
The utility model belongs to experimental equipment technical field, specifically, is related to a kind of for preparing plant protoplast
Enzymolysis liquid filter.
Background technology
Plant protoplast is a kind of preferable material for studying cell physiological function, is widely used in genetic transformation, thin
The fields such as born of the same parents are merged and cell body is mutated.The research of protoplast provides the research for genetic and cell engineering wide
Prospect.In the separation of protoplast, one of them important experimental procedure is the nylon wire using different pore size size
Filter membrane carries out filter operation to the enzymolysis liquid after enzymolysis, miscellaneous the purpose is to remove the plant tissue not digested completely and fragment
Matter.
Filtering for enzymolysis liquid, any specific filter is not had been reported that on the market, has been operated in actual experiment
In, usually directly fix nylon net filter film above centrifuge tube with hand and filter, or be aligned using the sieves of different pore size
The centrifuge tube mouth of pipe is filtered.But such operation is extremely inconvenient, such as：Nylon wire is directly touched with hand and easily causes protoplast
Pollution, be unfavorable for testing follow-up culture；Due to the small volume of enzymolysis liquid, generally less than 10ml, and sieves on the market
General volume is larger, in operation, easily spills a part of enzymolysis liquid, causes unnecessary loss；Also, due in enzymolysis liquid
Because there is stickiness than denser containing cellulase and pectase mostly, so when using both approaches, due to not having
The effect of pressure, it is difficult to be filled into centrifuge tube to make enzymolysis liquid.
Utility model content
In view of this, the utility model is directed to the problem of above-mentioned, there is provided a kind of enzyme for being used to prepare plant protoplast
Solve liquid filter.
In order to solve the above-mentioned technical problem, the utility model discloses a kind of enzymolysis liquid for being used to prepare plant protoplast
Filter, including funnel, the bottom of the funnel are provided with small opening pipeline, are provided with the inwall of the funnel detachable
Nylon net filter film, the small opening pipeline lower section are connected with hollow sleeve, are provided with the top of the sleeve coupled logical
Injection head, the small opening pipeline is inserted in injection head；Piston rod is provided with the sleeve, the other end of the piston rod is stretched
Go out outside the bottom of sleeve, lug is provided with the bottom of the sleeve outer wall.
Further, the funnel includes hollow cylindrical body, and the bottom of the staving is connected with conical bucket body,
The small opening pipeline is arranged at the center of bucket body bottom.
Further, projection is provided with the bottom of the inwall of the staving and in the intersection of staving and bucket body, it is described
Detachable nylon net filter film is fixed by projection and bucket body.
Further, projection is at least provided with 3.
Further, the funnel is prepared using high-temperature resistance plastice.
Further, the high-temperature resistance plastice is from polytetrafluoroethylene (PTFE), MPS, enhanced line style polyester or poly-
One kind in acid imide.
Further, it is provided with scale on the funnel and sleeve.
Further, a diameter of 2.5cm of the staving, a height of 3cm；A height of 0.8cm of small opening pipeline, it is a diameter of
0.2cm。
Further, the length of the injection head is 1cm, a diameter of 0.3cm.
Compared with prior art, the utility model can be obtained including following technique effect：
1) present apparatus is a small amount of enzymolysis liquid small design funnel, cylindrical hopper design, can prevent enzymolysis liquid from spreading
The loss of leakage.
2) present apparatus can freely replace the nylon wire of different pore size, be brought great convenience for the experiment in experiment.
3) present apparatus combines funnel with injection head, sleeve and piston rod, and in filtering, production is stretched out by piston rod
Raw pressure difference, the suction filtration to enzymolysis liquid is realized, make thick and sticking enzymolysis liquid be easy to pass through nylon net filter film, make reality
Test operation enhanced convenience.
Certainly, implement any product of the present utility model to it is not absolutely required to reach all the above technology effect simultaneously
Fruit.
Brief description of the drawings
Accompanying drawing described herein is used for providing further understanding to of the present utility model, forms one of the present utility model
Point, schematic description and description of the present utility model is used to explain the utility model, does not form to of the present utility model
Improper restriction.In the accompanying drawings：
Fig. 1 is the structural representation that the utility model is used to prepare the enzymolysis liquid filter of plant protoplast；
Fig. 2 is the structural representation that the utility model is used to prepare the funnel of the enzymolysis liquid filter of plant protoplast
Figure.
In figure, 1. funnels, 2. small opening pipelines, 3. sleeves, 4. injection heads, 5. detachable nylon net filter films, 6. scales,
7. piston rod, 8. lugs, 9. stavings, 10. bucket bodies, 11. projections.
Embodiment
Describe embodiment of the present utility model in detail below in conjunction with embodiment, thereby the utility model how should
It can be fully understood and implemented according to this to be solved technical problem and reach the implementation process of technical effect with technological means.
The utility model provides a kind of enzymolysis liquid filter for being used to prepare plant protoplast, as shown in figure 1, including
Funnel 1, the bottom of funnel 1 are provided with small opening pipeline 2, and detachable nylon net filter film 5 is provided with the inwall of funnel 1, leakage
The lower section of hole pipeline 2 is connected with hollow sleeve 3, and the top of sleeve 3 is provided with coupled logical injection head 4, and small opening pipeline 2 is inserted
In injection head 4；Piston rod 7 is provided with the sleeve 3, the other end of the piston rod 7 is stretched out outside the bottom of sleeve 3, institute
State and lug 8 is provided with the bottom of the outer wall of sleeve 3, prevent operator's finger slippage；Detachable nylon net filter film 5 can select
The nylon net filter film 5 of different pore size is selected, is brought great convenience for experiment；Injection head 4, sleeve 3 and piston rod 7 form one
Individual suction filtration mechanism, make thick and sticking enzymolysis liquid be easy to pass through nylon net filter film 5, make experimental implementation more convenient.
Funnel 1 includes hollow cylindrical body 9, and the bottom of the staving 9 is connected with conical bucket body 10, the small opening
Pipeline 2 is arranged at the center of the bottom of bucket body 10.Set on the bottom of the inwall of staving 9 and in the intersection of staving 9 and bucket body 10
Projection 11 is equipped with, projection is at least provided with 3；The detachable nylon net filter film 5 is fixed by projection 11 and bucket body 10.
Wherein, hollow cylindrical body 9 prevent the spilling of enzymolysis liquid, adhere to caused by waste；The side wall of conical bucket body 10 is
Inclined-plane, enzymolysis liquid is facilitated to be flowed to small opening pipeline.Nylon net filter film 5 is flexible, can be folded, still, when testing,
If without fixation, nylon net filter film 5 easily upwarps, and makes to form seam between nylon net filter film 5 and cylindrical body 9
Gap, cause the enzymolysis liquid of bulky grain to flow into the enzymolysis liquid of lower section smaller particle, influence filter effect, and conical bucket body 10
Side wall is inclined-plane, and its side wall forms acute angle with projection 11, can fix detachable nylon net filter film 5.
Funnel 1 is prepared using high-temperature resistance plastice.High-temperature resistance plastice is from polytetrafluoroethylene (PTFE), MPS, increasing
One kind in strong type line style polyester or polyimides.Scale 6 is provided with funnel 1 and sleeve 3.Staving 9 it is a diameter of
2.5cm, a height of 3cm；A height of 0.8cm of small opening pipeline 2, a diameter of 0.2cm.The length of injection head 4 is 1cm, a diameter of
0.3cm。
When in use, the detachable nylon net filter film 5 in aperture required for taking is placed on the lower section of projection 11, passes through projection
11 and bucket body 10 be fixed, by (i.e. close to injection head 4 at) at the minimum scale of the heap of piston rod 7 to sleeve 3, by small opening pipeline
2 are inserted in injection head 4, and the enzymolysis liquid of separated protoplast is poured into funnel 1, and operator is pressed from both sides with the middle finger and forefinger for suppressing hand
Firmly sleeve 3, held with another hand by piston rod 7, pull down piston rod 7, piston rod 7 moves downward in sleeve 3, is formed
Suction filtration acts on, and enzymolysis liquid is filled into sleeve 3, rear to inject in centrifuge tube, and now, lug 8 fixes middle finger and forefinger, prevents hand
Refer to and skid, then carry out follow-up centrifugal purification operation；When needing the detachable nylon net filter film 5 of different pore size, and
Shi Genghuan.
Some preferred embodiments of utility model have shown and described in described above, but as previously described, it should be understood that it is practical
It is new to be not limited to form disclosed herein, the exclusion to other embodiment is not to be taken as, and available for various other
Combination, modification and environment, and above-mentioned teaching or the skill of association area can be passed through in utility model contemplated scope described herein
Art or knowledge are modified.And the change and change that those skilled in the art are carried out do not depart from the spirit and scope of utility model, then
All should be in the protection domain of utility model appended claims.
Claims (9)
1. a kind of enzymolysis liquid filter for being used to prepare plant protoplast, it is characterised in that including funnel (1), the leakage
The bottom of bucket (1) is provided with small opening pipeline (2), and detachable nylon net filter film is provided with the inwall of the funnel (1)
(5) hollow sleeve (3), is connected with below the small opening pipeline (2), is provided with the top of the sleeve (3) coupled logical
Injection head (4), the small opening pipeline (2) is inserted in injection head (4)；Piston rod (7) is provided with the sleeve (3), it is described
The other end of piston rod (7) is stretched out outside the bottom of sleeve (3), and lug (8) is provided with the bottom of sleeve (3) outer wall.
2. the enzymolysis liquid filter according to claim 1 for being used to prepare plant protoplast, it is characterised in that described
Funnel (1) includes hollow cylindrical body (9), and the bottom of the staving (9) is connected with conical bucket body (10), the small opening
Pipeline (2) is arranged at the center of bucket body (10) bottom.
3. the enzymolysis liquid filter according to claim 2 for being used to prepare plant protoplast, it is characterised in that described
Projection (11) is provided with the bottom of the inwall of staving (9) and in the intersection of staving (9) and bucket body (10), it is described detachable
Nylon net filter film (5) is fixed by projection (11) and bucket body (10).
4. the enzymolysis liquid filter according to claim 3 for being used to prepare plant protoplast, it is characterised in that described
Projection (11) is at least provided with 3.
5. the enzymolysis liquid filter according to claim 1 for being used to prepare plant protoplast, it is characterised in that described
Funnel (1) is prepared using high-temperature resistance plastice.
6. the enzymolysis liquid filter according to claim 5 for being used to prepare plant protoplast, it is characterised in that described
One kind that high-temperature resistance plastice is selected in polytetrafluoroethylene (PTFE), MPS, enhanced line style polyester or polyimides.
7. the enzymolysis liquid filtering dress for being used to prepare plant protoplast according to any claim in claim 1-6
Put, it is characterised in that be provided with scale (6) on the funnel (1) and sleeve (3).
8. the enzymolysis liquid filter according to claim 2 for being used to prepare plant protoplast, it is characterised in that described
A diameter of 2.5cm of staving (9), a height of 3cm；A height of 0.8cm of small opening pipeline (2), a diameter of 0.2cm.
9. the enzymolysis liquid filtering dress for being used to prepare plant protoplast according to any claim in claim 1-5
Put, it is characterised in that the length of the injection head (4) is 1cm, a diameter of 0.3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720525722.8U CN206751794U (en) | 2017-05-11 | 2017-05-11 | A kind of enzymolysis liquid filter for being used to prepare plant protoplast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720525722.8U CN206751794U (en) | 2017-05-11 | 2017-05-11 | A kind of enzymolysis liquid filter for being used to prepare plant protoplast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206751794U true CN206751794U (en) | 2017-12-15 |
Family
ID=60618091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720525722.8U Expired - Fee Related CN206751794U (en) | 2017-05-11 | 2017-05-11 | A kind of enzymolysis liquid filter for being used to prepare plant protoplast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206751794U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109928460A (en) * | 2018-07-26 | 2019-06-25 | 中国科学院水生生物研究所 | A kind of portable planktonic organism enrichment facility |
-
2017
- 2017-05-11 CN CN201720525722.8U patent/CN206751794U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109928460A (en) * | 2018-07-26 | 2019-06-25 | 中国科学院水生生物研究所 | A kind of portable planktonic organism enrichment facility |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171215 Termination date: 20180511 |