CN206621998U - A kind of stem cell conditioned medium purification devices - Google Patents
A kind of stem cell conditioned medium purification devices Download PDFInfo
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- CN206621998U CN206621998U CN201720331219.9U CN201720331219U CN206621998U CN 206621998 U CN206621998 U CN 206621998U CN 201720331219 U CN201720331219 U CN 201720331219U CN 206621998 U CN206621998 U CN 206621998U
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- filter membrane
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- pipe
- stem cell
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Abstract
The utility model discloses a kind of stem cell conditioned medium purification devices, including：Support frame；Semiconductor cooling bench, is arranged on support frame；Cup is concentrated, is arranged on semiconductor cooling bench；Drive device, it includes peristaltic pump and stretches into the concentration bottom of a cup portion through the first flexible pipe and the second flexible pipe on the peristaltic pump, one end of the first flexible pipe；0.45 μm of filter membrane device, its sample introduction end are connected with the other end of first flexible pipe, and 0.45 μm of filter membrane is provided with inside 0.45 μm of filter membrane device；First processing unit, it is arranged on the semiconductor cooling bench and connected with 0.45 μm of filter membrane device sample outlet end；Second processing device, it is arranged on the semiconductor cooling bench.A kind of stem cell conditioned medium purification devices provided by the utility model, its is easy to use, reduces human body and is contacted with the direct of instrument, so as to effectively prevent the situation that human body pollutes to culture medium.
Description
Technical field
Biomedical sector is the utility model is related to, more specifically, the utility model is related to a kind of stem cell condition training
Support base purification devices.
Background technology
With the development of modern biomedical, the culture of stem cell plays indispensable work in biomedical research
With, and the quality of culture medium directly decides the quality of stem cell culture, at present, the purifying one to stem cell conditioned medium
As using refiltering the method for concentration after supernatant centrifugation, but lack the dress of the whole series in the filtering and concentrating to supernatant at present
Put, which results in the disorderly and unsystematic of instrument, easily error；In addition, because human body and instrument are indirect in operation
Touch relatively frequently, so culture medium can be caused to be contaminated to a certain extent.
Utility model content
A purpose of the present utility model is that solve at least the above, and provides the advantages of at least will be described later.
To achieve these goals, the utility model provides a kind of stem cell conditioned medium purification devices, including：
Support frame；
Semiconductor cooling bench, it is arranged on support frame as described above；
Cup is concentrated, it is arranged on the semiconductor cooling bench；
Drive device, it includes peristaltic pump and through the first flexible pipe and the second flexible pipe on the peristaltic pump, and described
The concentration bottom of a cup portion is stretched into one end of one flexible pipe；
0.45 μm of filter membrane device, its sample introduction end connect with the other end of first flexible pipe, inside 0.45 μm of filter membrane device
Provided with 0.45 μm of filter membrane；
First processing unit, it is arranged on the semiconductor cooling bench and connected with 0.45 μm of filter membrane device sample outlet end
It is logical, first support that first processing unit includes being arranged on the semiconductor cooling bench, it is arranged at the first support
On the first filter membrane pipe, the first waste liquid bottle and sample injection bottle that are arranged on the semiconductor cooling bench, on the first filter membrane pipe
Provided with the first sampling valve, the first sample output valve, the first return valve, tangentially filtered provided with 100KD doughnuts in the first filter membrane pipe
Film, first waste liquid bottle are located at immediately below first return valve and have conduit to connect between first return valve, institute
State sample injection bottle to be located at immediately below first sample output valve and there is conduit to connect between first sample output valve, first backflow
Valve is between first sampling valve and the tangential filter membrane of 100KD doughnuts；
Second processing device, it is arranged on the semiconductor cooling bench, and the second processing device includes being arranged at institute
The second filter membrane pipe for state the second support on semiconductor cooling bench, being arranged in the second support, it is arranged at the semiconductor
Receiving flask and the second waste liquid bottle on cooling bench, the second filter membrane pipe be provided with the second sampling valve, the second sample output valve, second time
Valve, the second flexible pipe are flowed, the tangential filter membrane of 5KD doughnuts is provided with the second filter membrane pipe, the receiving flask is located at described second
Immediately below return valve and there is conduit to connect between second return valve, second waste liquid bottle is located at second sample output valve
Underface and there is conduit to connect between second sample output valve, described second flexible pipe one end connected with second sampling valve and
Its other end stretches into the sample injection bottle bottom, and second return valve is located at second sampling valve and the 5KD doughnuts
Between tangential filter membrane.
Preferably, 0.45 μm of filter membrane diameter is identical with 0.45 μm of filter membrane device inside diameter.
Preferably, the tangential filter membrane diameter of the 100KD doughnuts is identical with the first filter membrane pipe diameter, described
The tangential filter membrane diameter of 5KD doughnuts is identical with the second filter membrane pipe diameter.
Preferably, support frame as described above is highly 5-20cm.
Preferably, the height of the first support and second support is all higher than first waste liquid bottle, sample injection bottle, collection
The height of bottle and the second waste liquid bottle.
Preferably, it is equipped with support arm in the first support and the second support.
The utility model comprises at least following beneficial effect：
1st, a kind of stem cell conditioned medium purification devices described in the utility model, it is readily transported and carried, and uses
It is convenient；
2nd, a kind of stem cell conditioned medium purification devices described in the utility model, greatly reduce people in use
Body directly contacts with instrument, so as to effectively prevent the situation that human body pollutes to culture medium.
Further advantage, target and feature of the present utility model embody part by following explanation, and part will also pass through
Research of the present utility model and practice are understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 is the structural representation of stem cell conditioned medium purification devices described in the utility model；
Fig. 2 is the structural representation of 0.45 μm of filter membrane device of stem cell conditioned medium purification devices described in the utility model
Figure；
Fig. 3 is the structural representation of the first filter membrane pipe of stem cell conditioned medium purification devices described in the utility model
Figure；
Fig. 4 is the structural representation of the second filter membrane pipe of stem cell conditioned medium purification devices described in the utility model
Figure；
Fig. 5 is the structural representation of the first support of stem cell conditioned medium purification devices described in the utility model；
1. peristaltic pump in figure, 2. semiconductor cooling benches, 3. support frames, 4.5KD hollow fiber filter membranes, the hollow fibres of 5.100KD
Tangential filter membrane is tieed up, 6. second flexible pipes, 7. first flexible pipes, 8.0.45 μm of filter membrane device, 9. receiving flasks, 10. concentration cups, 11. first is useless
Liquid bottle, 12. first supports, 13. sample injection bottles, 14. second waste liquid bottles, 15. desktops, 16. support arms, 17. first filter membrane pipes, 18.
Second filter membrane pipe, 19. first sampling valves, 20. first sample output valves, 21. first return valves, 22. second sampling valves, 23. second go out
Sample valve, 24. second return valves, 25.0.45 μm of filter membrane.
Embodiment
Below in conjunction with the accompanying drawings and embodiment is described in further detail to the utility model, to make people in the art
Member can implement according to this with reference to specification word.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded from one or more
The presence or addition of individual other elements or its combination.
As shown in Figures 1 to 4, one kind is provided in the one of embodiment of the utility model, including：
Support frame 3, support frame as described above are placed on desktop 15；
Semiconductor cooling bench 2, it is arranged on support frame as described above 3；
Cup 10 is concentrated, it is arranged on the semiconductor cooling bench 2；
Drive device, it includes peristaltic pump 1 and through the first flexible pipe 7 and the second flexible pipe 6 on the peristaltic pump 1, institute
Concentration cup 10 bottom is stretched into the one end for stating the first flexible pipe 7；
0.45 μm of filter membrane device 8, its sample introduction end connect with the other end of first flexible pipe 7, in 0.45 μm of filter membrane device 8
Portion is provided with 0.45 μm of filter membrane 25；
First processing unit, it is arranged on the semiconductor cooling bench 2 and connected with 0.45 μm of filter membrane device 8 sample outlet end
It is logical, first support 12 that first processing unit includes being arranged on the semiconductor cooling bench 2, it is arranged at described first
The first filter membrane pipe 17 on frame 12, the first waste liquid bottle 11 and sample injection bottle 13 being arranged on the semiconductor cooling bench 2, described
One filter membrane pipe 17 is provided with the first sampling valve 19, the first sample output valve 20, the first return valve 21, is provided with the first filter membrane pipe 17
The tangential filter membrane 5 of 100KD doughnuts, first waste liquid bottle 11 is immediately below first return valve 21 and with described first
There is conduit connection between return valve 21, the sample injection bottle 13 goes out sample immediately below first sample output valve 20 and with described first
There is conduit connection between valve 20, first return valve 21 is located at first sampling valve 19 and the 100KD doughnuts are cut
To between filter membrane 5；
Second processing device, it is arranged on the semiconductor cooling bench 2, and the second processing device includes being arranged at institute
The second filter membrane pipe 18 for state the second support on semiconductor cooling bench 2, being arranged in the second support, it is arranged at and described partly leads
The waste liquid bottle 14 of receiving flask 9 and second on body cooling bench 2, the second filter membrane pipe 18 go out provided with the second sampling valve 22, second
Sample valve 23, the second return valve 24, the second flexible pipe 6, the second filter membrane pipe 18 is interior to be provided with the tangential filter membrane 4 of 5KD doughnuts, described
Receiving flask 9 immediately below second return valve 24 and has conduit to connect between second return valve 24, and described second
Waste liquid bottle 14 immediately below second sample output valve 23 and has conduit to connect between second sample output valve 23, and described second
The one end of flexible pipe 6 connects with second sampling valve 22 and its other end stretches into the bottom of sample injection bottle 13, second return valve
24 between second sampling valve 22 and the tangential filter membrane 4 of the 5KD doughnuts.
0.45 μm of diameter of filter membrane 25 is identical with 0.45 μm of filter membrane device 8 inside diameter.
The tangential diameter of filter membrane 5 of 100KD doughnuts is identical with 17 diameters of the first filter membrane pipe, and the 5KD is hollow
The tangential diameter of filter membrane 4 of fiber is identical with 18 diameters of the second filter membrane pipe.
The height of support frame as described above 3 is 5-20cm.
The height of the support 12 is more than first waste liquid bottle 11, sample injection bottle 13, the waste liquid bottle 14 of receiving flask 9 and second
Height.
As shown in figure 5, it is equipped with support arm 16, second support and first support in the first support 12 and second support
12 structure is identical.
Culture medium purge process：Defrosting supernatant, take out supernatant from -40 DEG C of refrigerators within 48 hours in advance, be put into 4 DEG C of ice
Case；Centrifugation, supernatant is transferred in 50mL centrifuge tube, and 4000 rotary speeds centrifuge 5 minutes；The supernatant for centrifuging completion is fast
Speed is transferred in the concentration cup 10.
According to foregoing description, the peristaltic pump 1 is opened, the peristaltic pump 1 is by extruding the first flexible pipe 7 by the concentration cup
Supernatant in 10, which squeezes, to be pumped into 0.45 μm of filter membrane device 8, and 0.45 μm of filter membrane 25 filters to supernatant, removes it
Middle cell fragment, the supernatant after filtering enter the first filter membrane pipe 17 by first sampling valve 19, and liquid flows through institute
The tangential filter membrane 5 of 100KD doughnuts is stated, the foreign protein more than 100KD flows into first waste liquid through first return valve 21
In bottle 11, albumen less than 100KD by the tangential sample output valve 20 of filter membrane 5 and first of the 100KD doughnuts flow into it is described enter
Sample bottle 13, now, supernatant only exists the albumen that atomic mass is less than 100KD in sample injection bottle 13；Then, the peristaltic pump 1 is logical
Cross the second flexible pipe 6 and the supernatant in the sample injection bottle 13 is pumped into the second filter membrane pipe 18, liquid flows through the hollow fibres of the 5KD
Filter membrane 4 is tieed up, albumen of the diameter more than 5KD is flowed into the receiving flask 9 by second return valve 24, and diameter is less than 5KD's
Material flows into 14 bottles of second waste liquid by the 5KD hollow fiber filter membranes 4 and second sample output valve 23, now, described
Supernatant in receiving flask 9 only exists albumen of the atomic mass between 5KD-100KD.
Although embodiment of the present utility model is disclosed as above, it is not restricted in specification and embodiment
Listed utilization, it can be applied to various suitable fields of the present utility model completely, for those skilled in the art,
Other modification is easily achieved, therefore under the universal limited without departing substantially from claim and equivalency range, this reality
Specific details is not limited to new and shown here as the legend with description.
Claims (6)
- A kind of 1. stem cell conditioned medium purification devices, it is characterised in that including：Support frame；Semiconductor cooling bench, it is arranged on support frame as described above；Cup is concentrated, it is arranged on the semiconductor cooling bench；Drive device, it includes peristaltic pump and through the first flexible pipe and the second flexible pipe on the peristaltic pump, and described first is soft The concentration bottom of a cup portion is stretched into one end of pipe；0.45 μm of filter membrane device, its sample introduction end connect with the other end of first flexible pipe, are provided with inside 0.45 μm of filter membrane device 0.45 μm of filter membrane；First processing unit, it is arranged on the semiconductor cooling bench and connected with 0.45 μm of filter membrane device sample outlet end, institute State the first support that the first processing unit includes being arranged on the semiconductor cooling bench, be arranged in the first support the One filter membrane pipe, the first waste liquid bottle and sample injection bottle being arranged on the semiconductor cooling bench, the first filter membrane pipe are provided with the One sampling valve, the first sample output valve, the first return valve, the first filter membrane pipe is interior to be provided with the tangential filter membrane of 100KD doughnuts, described First waste liquid bottle is located at immediately below first return valve and has conduit to connect between first return valve, the sample injection bottle There is conduit to connect immediately below first sample output valve and between first sample output valve, first return valve is located at institute State between the first sampling valve and the tangential filter membrane of 100KD doughnuts；Second processing device, it is arranged on the semiconductor cooling bench, and the second processing device includes being arranged at described half Second support on conductor cooling bench, the second filter membrane pipe being arranged in the second support, it is arranged at the semiconductor cooling Receiving flask and the second waste liquid bottle on platform, the second filter membrane pipe are provided with the second sampling valve, the second sample output valve, the second backflow Valve, the second flexible pipe, the second filter membrane pipe is interior to be provided with the tangential filter membrane of 5KD doughnuts, and the receiving flask is located at described second time Immediately below stream valve and there is conduit to connect between second return valve, second waste liquid bottle is being located at second sample output valve just Lower section and there is conduit to connect between second sample output valve, described second flexible pipe one end is connected with second sampling valve and it The other end stretches into the sample injection bottle bottom, and second return valve is located at second sampling valve and the 5KD doughnuts are cut To between filter membrane.
- A kind of 2. stem cell conditioned medium purification devices according to claim 1, it is characterised in that 0.45 μm of filter Film diameter is identical with 0.45 μm of filter membrane device inside diameter.
- 3. a kind of stem cell conditioned medium purification devices according to claim 1, it is characterised in that in the 100KD The tangential filter membrane diameter of hollow fiber is identical with the first filter membrane pipe diameter, the tangential filter membrane diameter of 5KD doughnuts with it is described Second filter membrane pipe diameter is identical.
- 4. a kind of stem cell conditioned medium purification devices according to claim 1, it is characterised in that support frame as described above is high Spend for 5-20cm.
- A kind of 5. stem cell conditioned medium purification devices according to claim 1, it is characterised in that the first support The height of first waste liquid bottle, sample injection bottle, receiving flask and the second waste liquid bottle is all higher than with the height of second support.
- A kind of 6. stem cell conditioned medium purification devices according to claim 1, it is characterised in that the first support Support arm is equipped with the second support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720331219.9U CN206621998U (en) | 2017-03-31 | 2017-03-31 | A kind of stem cell conditioned medium purification devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720331219.9U CN206621998U (en) | 2017-03-31 | 2017-03-31 | A kind of stem cell conditioned medium purification devices |
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| Publication Number | Publication Date |
|---|---|
| CN206621998U true CN206621998U (en) | 2017-11-10 |
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ID=60206055
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720331219.9U Active CN206621998U (en) | 2017-03-31 | 2017-03-31 | A kind of stem cell conditioned medium purification devices |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115773225A (en) * | 2022-11-30 | 2023-03-10 | 苏州赛赋新药技术服务有限责任公司 | Stem cell culture perfusion device |
-
2017
- 2017-03-31 CN CN201720331219.9U patent/CN206621998U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115773225A (en) * | 2022-11-30 | 2023-03-10 | 苏州赛赋新药技术服务有限责任公司 | Stem cell culture perfusion device |
| CN115773225B (en) * | 2022-11-30 | 2023-11-03 | 苏州灵思生物科技有限公司 | Stem cell culture perfusion device |
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