CN106834422A - Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule - Google Patents
Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule Download PDFInfo
- Publication number
- CN106834422A CN106834422A CN201710029497.3A CN201710029497A CN106834422A CN 106834422 A CN106834422 A CN 106834422A CN 201710029497 A CN201710029497 A CN 201710029497A CN 106834422 A CN106834422 A CN 106834422A
- Authority
- CN
- China
- Prior art keywords
- bottle
- manipulator
- flexible pipe
- peristaltic pump
- collection bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of full-automatic streamline collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule, including the first manipulator, ampoule bottle box, first flexible pipe installs auxiliary body, second manipulator, first peristaltic pump, surge tank/profit film liquid/cleaning fluid/medium bottle box, second peristaltic pump, flexible pipe current limliting/heat sealing mechanism, 3rd manipulator, collection bacterium bottle rack, yellow cap stopper, red cap stopper, 4th manipulator, flexible pipe the like waste recovery area, 5th manipulator, second flexible pipe installs auxiliary body, 6th manipulator, collection bacterium bottle/flexible pipe/cap stopper box, 7th manipulator, 8th manipulator, ampoule bottle opener, the recovery areas such as reagent bottle.The present invention uses multipaths string, parallel optimization method to the bottled aqueous solution test sample of ampoule to be checked, realizes that continuous, efficient microorganism is enriched with automatically, greatly improves operating efficiency, and reduce flase drop risk.
Description
Technical field
The invention belongs to sterility test system automation technical field, it is related to a kind of towards the bottled aqueous solution test sample of ampoule
Full-automatic streamline collection bacterium operating method and system.
Technical background
According to version in 2015《Chinese Pharmacopoeia》, the Sterility Test of test sample includes membrane-filter procedure and direct inoculation.
Membrane-filter procedure should typically use closed membrane filter.The filter sizes of sterility test should be not more than 0.45 μm, diameter
About 50mm.Test sample includes：Aqueous solution test sample, water-soluble solid test sample, water-insoluble test sample, dissolve in the tetradecane
Descendants' agent of isopropyl propionate and stickiness finish test sample, aseptic gas（Spray）Mist agent test sample, the syringe test sample equipped with medicine, tool
There is the medical apparatus of conduit（Blood transfusion, infusion bag etc.）Test sample etc..Meanwhile,《Chinese Pharmacopoeia》Amount of inspection and inspection to test sample
The amount of testing also is made that clearly regulation.It can be seen that, be faced with during Sterility testing Multi-example species, many specification pieces packaging,
Multipaths process analysis procedure analysis, noncontinuity operation, the problem of low collection bacterium analysis efficiency.
During existing Sterility testing, Multi-example species, many specification pieces packaging, multipaths process point are faced with
Analysis, noncontinuity operation, the problem of low collection bacterium analysis efficiency, it is common to use manual mode completes associative operation, automation, intelligence
Change degree is relatively low；The factors that are influenceed by personnel of critical process such as the operation of collection bacterium, cleaning are larger, also can because of different human users band
Carry out the significant difference of sterility test result and efficiency.
The content of the invention
The problem that associative operation is present is completed for manual mode during Sterility testing, the present invention is proposed towards ampoule bottle
Fill aqueous solution test sample full-automatic streamline collection bacterium operating method and system, it is characterised in that using string, parallel processing and
Flow optimization method, comprises the following steps：
Step（1）Ampoule bottle box, surge tank/profit film liquid/cleaning fluid/medium bottle box on gripping guide rail, collect bacterium bottle/flexible pipe/
Cap stopper box is respectively put into fixed position.
Step（2）Collection bacterium bottle/flexible pipe/cap stopper box is opened, the flexible pipe with syringe needle being connected with collection bacterium bottle is installed into second
Peristaltic pump, while the another set of flexible pipe with syringe needle in box is installed into the first peristaltic pump.
Step（3）The flexible pipe with syringe needle being connected with collection bacterium bottle is installed into flexible pipe current limliting/heat sealing mechanism；Bacterium will be collected simultaneously
Bottle is put into collection bacterium bottle rack, and the yellow cap stopper, red cap stopper in collection bacterium bottle/flexible pipe/cap stopper box are put into fixed position.
Step（4）The syringe needle on flexible pipe that will be arranged in the second peristaltic pump inserts surge tank/profit film liquid/cleaning fluid/training
Support base bottle box in profit film liquid bottle on, be inverted profit film liquid bottle after open the second peristaltic pump, will profit film liquid pump into collection bacterium bottle in micro-
Hole filter membrane is soaked, and the second peristaltic pump is closed after profit film liquid reaches certain capacity.
Step（5）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting profit film liquid bottle, gas is pumped into
It is pressurized in collection bacterium bottle so that profit film liquid from collection bacterium bottle discharge, the second peristaltic pump is closed after profit film liquid is completely exhausted out completely,
Remove red cap stopper to fixed position.
Step（6）In step（2）To step（5）While carrying out, the ampoule bottle in ampoule bottle box is sent to ampoule bottle and is opened
Open and opened in mechanism, unlatching sends back in ampoule bottle box after terminating.
Step（7）In step（6）While carrying out, the syringe needle insertion on the flexible pipe one end that will be arranged in the first peristaltic pump
In the ampoule bottle having turned on, the buffering in the syringe needle insertion surge tank/profit film liquid/cleaning fluid/medium bottle box of the flexible pipe other end
On tank, the first peristaltic pump of unlatching pumps into the aqueous solution test sample in ampoule bottle in surge tank；Treat that the aqueous solution in ampoule bottle is supplied
After test product pumps into surge tank completely, the first peristaltic pump is closed；Syringe needle is removed the peace that the ampoule bottle for having finished has turned on to other
In small jar bottle, the first peristaltic pump of unlatching pumps into the aqueous solution test sample in ampoule bottle in surge tank.
Step（8）After the aqueous solution test sample in the ampoule bottle of all feedings pumps into surge tank completely, by profit film liquid bottle
On syringe needle extract and insert on surge tank, be inverted surge tank and open the second peristaltic pump, by aqueous solution test sample pump into collect bacterium
In bottle, the second peristaltic pump is closed after aqueous solution test sample is transferred in collection bacterium bottle completely.
Step（9）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting surge tank, gas is pumped into collection
It is pressurized in bacterium bottle, by collecting, the miillpore filter in bacterium bottle is filtered to aqueous solution test sample and microorganism is enriched with, and works as the aqueous solution
Test sample closes the second peristaltic pump from after collection bacterium bottle discharge completely, removes red cap stopper to fixed position.
Step（10）Syringe needle on surge tank is extracted and inserted on cleaning fluid bottle, cleaning fluid bottle is inverted and unlatching second is compacted
Dynamic pump, during cleaning fluid pumped into collection bacterium bottle, closes the second peristaltic pump after cleaning fluid reaches certain capacity.
Step（11）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting cleaning fluid bottle, by gas pump
Enter to collect and be pressurized in bacterium bottle, cleaning fluid is discharged from collection bacterium bottle and realizes cleaning to miillpore filter, when cleaning fluid is completely exhausted out
After close the second peristaltic pump, remove red cap stopper to fixed position.
Step（12）Yellow cap stopper is moved into the bottom of collection bacterium bottle and is pressed into, sealed, prevent water clock.
Step（13）Syringe needle on cleaning fluid bottle is extracted and inserted on the medium bottle equipped with a certain culture medium, while closing
Close with after second, third collection bacterium flexible pipe for being connected of bottle, be inverted medium bottle and the second peristaltic pump of unlatching, culture medium is pumped into the
In one collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Step（14）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with same medium, together
When close with after the first, the 3rd collection bacterium flexible pipe that is connected of bottle, medium bottle simultaneously the second peristaltic pump of unlatching is inverted, by culture medium pump
Enter in second collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Step（15）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with different culture media, together
When close with after the first, second collection bacterium flexible pipe that is connected of bottle, medium bottle simultaneously the second peristaltic pump of unlatching is inverted, by culture medium pump
Enter in the 3rd collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Step（16）Red cap stopper lid is entered to collect bacterium top of bottle, it is soft that cutting simultaneously seals three be connected with collection bacterium bottle simultaneously
Pipe.
Step（17）Surge tank/profit film liquid/cleaning fluid/medium bottle puts back to surge tank/profit film liquid/cleaning fluid/culture medium
In bottle box, while the flexible pipe of the first peristaltic pump and the second peristaltic pump upper band syringe needle is removed, all of discarded object is transferred to soft
Pipe the like waste recovery area.
Step（18）Reset all devices to original state, waits the automatic collection bacterium behaviour of next batch aqueous solution test sample
Make.
Furtherly, the opening time of the whole ampoule bottles in ampoule bottle box and whole aqueous solution test samples are transferred to buffering
The time sum of tank, should be less than being equal to peace of the flexible pipe being connected with collection bacterium bottle in the second peristaltic pump, flexible pipe current limliting/heat sealing mechanism
ETL estimated time of loading and the wetting, the efflux time sum that collect miillpore filter in bacterium bottle.
Furtherly, aqueous solution test sample species to be checked need to meet requirement to the flushing dose of cleaning fluid, if cleaning
Amount exceedes single bottle cleaning fluid, it is necessary to increase by two steps after step 11：
Step（a）Syringe needle on first cleaning fluid bottle is extracted and inserted on the second cleaning fluid bottle, the second cleaning fluid bottle is inverted simultaneously
Open the second peristaltic pump by cleaning fluid pump into collection bacterium bottle in, the second peristaltic pump is closed after cleaning fluid reaches certain capacity；
Step（b）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting the second cleaning fluid bottle, gas is pumped into
It is pressurized in collection bacterium bottle, cleaning fluid discharges the cleaning realized to miillpore filter from collection bacterium bottle, after cleaning fluid is completely exhausted out
The second peristaltic pump is closed, red cap stopper to fixed position is removed.
For the present invention towards the bottled aqueous solution test sample of ampoule full-automatic streamline collection bacterium operating system, including the first machine
Tool hand, ampoule bottle box, the first flexible pipe install auxiliary body, the second manipulator, the first peristaltic pump, surge tank/profit film liquid/cleaning
Liquid/medium bottle box, the second peristaltic pump, flexible pipe current limliting/heat sealing mechanism, the 3rd manipulator, collection bacterium bottle rack, yellow cap stopper, red cap
Plug, the 4th manipulator, flexible pipe the like waste recovery area, the 5th manipulator, the second flexible pipe install auxiliary body, the 6th manipulator,
The recovery areas such as collection bacterium bottle/flexible pipe/cap stopper box, the 7th manipulator, the 8th manipulator, ampoule bottle opener, reagent bottle.
First manipulator carries out cooperating with ampoule bottle opener：First manipulator takes the peace in ampoule bottle box
Small jar bottle, and place into ampoule bottle opener；Combining with ampoule bottle opener carries out ampoule bottle cutting, operation of breakage, real
Existing ampoule bottle is opened；After ampoule bottle is opened, the first manipulator puts back in ampoule bottle box ampoule bottle；Treat the aqueous solution in ampoule bottle
After test sample is transferred to surge tank, ampoule bottle discarded object is transferred to the recovery areas such as reagent bottle by the first manipulator.
Second manipulator and the 6th manipulator carry out cooperating：Second manipulator gripping surge tank, profit film liquid bottle,
Cleaning fluid bottle or medium bottle, and be put into surge tank/profit film liquid/cleaning fluid/medium bottle box；6th manipulator is gripped and collection
The syringe needle of the connected flexible pipe one end of bacterium bottle, and insert the needle into or extract surge tank, moisten film liquid bottle, cleaning fluid bottle or culture medium
Bottle, the second manipulator overturns to surge tank, profit film liquid bottle, cleaning fluid bottle or medium bottle；When surge tank, profit film liquid bottle,
Cleaning fluid bottle or medium bottle in solution be transferred to collection bacterium bottle after, the second manipulator by surge tank, profit film liquid bottle, cleaning fluid bottle
Or placed to recovery areas such as reagent bottles in medium bottle.
5th manipulator, the 6th manipulator and the second flexible pipe install auxiliary body and carry out cooperating：6th machinery
Hand grips the syringe needle of the flexible pipe one end being connected with collection bacterium bottle, the 5th manipulator gripping collection bacterium bottle, while stretching and mobile flexible pipe will
It is put into the second peristaltic pump, and the second flexible pipe installation auxiliary body is secured the hose in the second peristaltic pump.
5th manipulator, flexible pipe current limliting/heat sealing mechanism and the 3rd manipulator carry out cooperating：5th manipulator is pressed from both sides
Take collection bacterium bottle and stretch the flexible pipe being connected with collection bacterium bottle, the 3rd manipulator is by flexible pipe press-in flexible pipe current limliting/heat sealing mechanism；Will be soft
After pipe loads flexible pipe current limliting/heat sealing mechanism, the 5th manipulator will collect bacterium bottle and be put into collection bacterium bottle rack；When carrying out media transfer extremely
During collection bacterium bottle, flexible pipe current limliting/heat sealing mechanism carries out selective current limliting to flexible pipe, by media transfer to corresponding bacteria gathering device
In；After media transfer to collection bacterium bottle, the flexible pipe that flexible pipe current limliting/heat sealing mechanism will cut off and collection bacterium bottle is connected, and added
Heat or pinch seal；
5th manipulator, the 6th manipulator, flexible pipe current limliting/heat sealing mechanism and the 3rd manipulator carry out cooperating：When soft
After the flexible pipe that pipe current limliting/heat sealing mechanism cut-out is connected with collection bacterium bottle, the syringe needle of the flexible pipe after the gripping cut-out of the 6th manipulator will be soft
Pipe is transferred to flexible pipe the like waste recovery area.
7th manipulator, the 8th manipulator and the first flexible pipe install auxiliary body and carry out cooperating；7th machinery
The syringe needle of hand gripping flexible pipe one end, the 8th manipulator grips the syringe needle of the flexible pipe other end, while stretching and mobile flexible pipe are put
Enter in the first peristaltic pump, the first flexible pipe installation auxiliary body is secured the hose in the first peristaltic pump.
7th manipulator, the 8th manipulator and the second manipulator carry out cooperating：Second manipulator clamps buffering
Tank, the syringe needle of the 7th manipulator gripping flexible pipe one end, movement simultaneously inserts surge tank；8th manipulator grips the pin of the flexible pipe other end
Head, movement simultaneously inserts ampoule bottle, and the aqueous solution test sample in ampoule bottle is transferred in surge tank, water-soluble in current ampoule bottle
After the transfer of liquid test sample is finished, the 8th manipulator is extracted syringe needle, movement and is inserted and carry out in the ampoule bottle that other have turned on water-soluble
The transfer of liquid test sample, the aqueous solution test sample repeated to whole ampoule bottles is transferred in surge tank；Treat whole ampoules
After aqueous solution test sample in bottle is transferred in surge tank, the 7th manipulator extracts the syringe needle of flexible pipe one end, the 8th manipulator
The syringe needle of the flexible pipe other end is extracted, flexible pipe discarded object is transferred to flexible pipe the like waste recovery area by the 8th manipulator.
8th machinery carries out cooperating with the first peristaltic pump：When the 8th manipulator inserts the syringe needle of flexible pipe one end
After ampoule bottle, the first peristaltic pump is opened, carry out aqueous solution test sample transfer；When the aqueous solution test sample transfer in current ampoule bottle
To surge tank, the first peristaltic pump is closed；8th manipulator from current ampoule bottle extracts the syringe needle of flexible pipe one end and insert
Other have turned on ampoule bottle, open the first peristaltic pump, carry out the transfer of aqueous solution test sample；Circulate operation is until all ampoule bottles
Interior aqueous solution test sample is fully transferred in surge tank.
4th manipulator, the second peristaltic pump and the second manipulator carry out cooperating：4th manipulator will be from collection bacterium
Yellow cap stopper, red cap stopper are taken out in bottle/flexible pipe/cap stopper box, and is placed to fixed position；When carrying out profit film liquid, aqueous solution test sample
When being transferred to collection bacterium bottle with cleaning fluid, the second manipulator is inverted surge tank, profit film liquid bottle or cleaning fluid bottle, opens second and wriggles
Pump；After completion profit film liquid, aqueous solution test sample and cleaning fluid are transferred to collection bacterium bottle, the second peristaltic pump, the 4th manipulator are closed
Red cap stopper will be covered to collection bacterium bottle；After red cap stopper is covered, the second manipulator is just putting surge tank, profit film liquid bottle or cleaning fluid
Bottle or medium bottle, open the second peristaltic pump；After film liquid, aqueous solution test sample and cleaning fluid is moistened from collection bacterium bottle discharge, close
Second peristaltic pump, the 4th manipulator removes to fixed position red cap stopper from collection bacterium bottle；After the completion of collection bacterium bottle cleaning, the 4th
Manipulator is sealed the press-in collection bacterium bottom of bottle portion of yellow cap stopper, and the second manipulator is inverted medium bottle, opens the second peristaltic pump；
After three bottles of culture mediums are fully transferred to collection bacterium bottle, the second peristaltic pump is closed, the 4th manipulator will be covered into red cap stopper to collection bacterium bottle
On.
Furtherly, the collection bacterium bottle is capable of achieving filtering and the Enrichment of bacteria of aqueous solution test sample built with miillpore filter.
Furtherly, manipulator be with multivariant mechanical arm or with manipulator grab with identical function
Travel mechanism.
Compared with existing manual mode is operated, full-automatic streamline collection bacterium operating method proposed by the present invention and system,
Using string, parallel processing and flow optimization method, multiple manipulator coordinateds, the nothing of the aqueous solution test sample bottled to ampoule
Bacterium check process is standardized, medelling, and whole flow process is entirely controlled, it is to avoid conventional sterility test all relies on personnel behaviour
Make, as a result influenceed larger disadvantage by artificial origin, greatly improve the efficiency of sterility test and the accuracy rate of sterility test result
And confidence level.
Brief description of the drawings
The manual mode operating process of the bottled aqueous solution test sample of the typical ampoules of Fig. 1；
The automation collection bacterium operating process schematic diagram of the bottled aqueous solution test sample of Fig. 2 ampoules；
Modularizations and time optimization processing schematic diagram of the Fig. 3 for automation collection bacterium operating process；
Fig. 4 automation collection bacterium operation system function arrangement distribution schematic diagrams.
Specific embodiment
Implement to describe the present invention in detail below in conjunction with the accompanying drawings and most preferably.
Sterility test towards the bottled aqueous solution test sample of ampoule is analyzed, and typical manual mode operating process is shown in Fig. 1.From
The operating process can be seen that typically carries out the three bottles or several bottles sterility tests of aqueous solution test sample, and operating procedure is numerous
It is many.It is in order to ensure inspection efficiency higher therefore very high to the qualification requirement of operating personnel.In some cases, due to behaviour
Making the carelessness of personnel can cause the false judgment of inspection result, cause serious consequence.Therefore the present invention is proposed towards using complete
Automation mechanized operation is entered towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule to flow
Row is multi-level to be decomposed, and using string, parallel processing manner, modularized design completes automation mechanized operation using manipulator coordinated.
According to version in 2015《Chinese Pharmacopoeia》With version GMP in 2010《GMP》, for ampoule bottle
Dress aqueous solution test sample, by process optimization, its automation collection bacterium operating process schematic diagram is shown in Fig. 2.Per batch simultaneously to 30
The bottled aqueous solution test sample of ampoule is collecting bacteria operation, according to the overall procedure for moistening film-collection bacterium-cleaning-plus culture medium such as
Under：
（1）Flexible pipe is installed and each reagent bottle is placed, while opening ampoule bottle；
（2）Test sample pumps into surge tank by pipeline 2, peristaltic pump 1 in ampoule bottle；
（3）Profit film liquid pumps into collection bacterium bottle by pipeline 1, peristaltic pump 2, each collection bacterium bottle about 50ml；
（4）Red cap stopper compresses collection bacterium bottle, and air pumps into collection bacterium bottle by pipeline 2, peristaltic pump 2 in sample bottle, until profit film liquid is complete
Full discharge；
（5）Red cap stopper is removed, test sample pumps into collection bacterium bottle from surge tank by pipeline 2, peristaltic pump 2, each collection bacterium bottle is about
100ml；
（6）Red cap stopper compresses sealing collection bacterium bottle, and air pumps into collection bacterium bottle by pipeline 2, peristaltic pump 2 in sample bottle, until collection bacterium
Filtering terminates；
（7）Red cap stopper is removed, cleaning fluid pumps into collection bacterium bottle by pipeline 2, peristaltic pump 2, each collection bacterium bottle about 100ml；
（8）Red cap stopper compresses sealing collection bacterium bottle, and air pumps into collection bacterium bottle by pipeline 2, peristaltic pump 2 in cleaning fluid bottle, until clearly
Washing lotion is completely exhausted out；
（9）Red cap stopper is removed, press-in yellow cap stopper in collection bacterium bottom of bottle portion closes B, C pipeline, and culture medium 1 passes through pipeline 3, the pump of peristaltic pump 2
Enter to collect 1 about 100ml of bacterium bottle；
（10）A, C pipeline are closed, culture medium 2 pumps into collection bacterium bottle 2, about 100ml by pipeline 3, peristaltic pump 2；
（11）A, B pipeline are closed, culture medium 2 pumps into collection bacterium bottle 3, about 100ml by pipeline 3, peristaltic pump 2；
（12）Red cap stopper compresses sealing collection bacterium bottle, cuts A, B, C pipeline, and seal or clamp collection bacterium bottle A, B, C pipeline；
（13）Flexible pipe and each reagent bottle are removed, recovery area is moved to.
In order to shorten the operating time, improve operating efficiency, the present invention towards ampoule bottle to filling the automatic of aqueous solution test sample
Changing collection fungus strain system carries out sub-module treatment, sees Fig. 3.The system includes：Packaging opening module, flexible pipe are installed module 1, flexible pipe and are installed
Module 2, reagent bottle and cap stopper placement module, ampoule bottle opening module, reagent bottle movement and flip module, syringe needle plug-in module,
Cap stopper operation module, culture medium filling current limliting module, heat-sealing and shear module and flexible pipe dismounting and reagent bottle discard module.
Automation collection bacterium is carried out using Multi-freedom-degreemanipulator manipulator coordinated to operate, the function of whole automation operating system
Arrangement distribution is shown in that Fig. 4, its operating procedure are：
（1）Ampoule bottle box 2, surge tank/profit film liquid/cleaning fluid/medium bottle box 6, collection bacterium bottle/flexible pipe/cap on gripping guide rail
Plug box 18 is respectively put into fixed position.
（2）Collection bacterium bottle/flexible pipe/cap stopper box 18 is opened, the flexible pipe with syringe needle being connected with collection bacterium bottle is installed compacted into second
Dynamic pump 7；The another set of flexible pipe with syringe needle in box is installed into the first peristaltic pump 5 simultaneously.
（3）The flexible pipe with syringe needle being connected with collection bacterium bottle is installed into flexible pipe current limliting/heat sealing mechanism 8；Simultaneously will collection bacterium bottle
It is put into collection bacterium bottle rack 10, the yellow cap stopper 11, red cap stopper 12 in collection bacterium bottle/flexible pipe/cap stopper box 18 are put into fixed position；
（4）The syringe needle on flexible pipe that will be arranged in the second peristaltic pump 7 inserts surge tank/profit film liquid/cleaning fluid/medium bottle box
In 6 profit film liquid bottle on, be inverted profit film liquid bottle after open the second peristaltic pump 7, will profit film liquid pump into collection bacterium bottle in miillpore filter
Soaked, the second peristaltic pump 7 is closed after profit film liquid reaches certain capacity；
（5）Red cap stopper 12 is covered into collection bacterium top of bottle, the second peristaltic pump 7 is opened after just putting profit film liquid bottle, gas is pumped into collection bacterium
It is pressurized in bottle so that profit film liquid from collection bacterium bottle discharge, the second peristaltic pump 7 is closed after profit film liquid is completely exhausted out completely, is moved
Become much more popular cap stopper 12 to fixed position；
（6）While step 2 to step 5 is carried out, the ampoule bottle in ampoule bottle box 2 is sent in ampoule bottle opener 21
Opened, unlatching sends back in ampoule bottle box 2 after terminating；
（7）While step 6 is carried out, what the syringe needle insertion on the flexible pipe one end that will be arranged in the first peristaltic pump 5 had turned on
In ampoule bottle, on the surge tank in the syringe needle insertion surge tank/profit film liquid/cleaning fluid/medium bottle box 6 of the flexible pipe other end, open
Open during the aqueous solution test sample in ampoule bottle pumps into surge tank by the first peristaltic pump 5；Treat that the aqueous solution test sample in ampoule bottle is complete
After pumping into surge tank entirely, the first peristaltic pump 5 is closed；Syringe needle is removed the ampoule bottle that the ampoule bottle for having finished has turned on to other
In, the first peristaltic pump 5 of unlatching pumps into the aqueous solution test sample in ampoule bottle in surge tank；
（8）After the aqueous solution test sample in the ampoule bottle of all feedings pumps into surge tank completely, the syringe needle on film liquid bottle will be moistened
Extract and insert on surge tank, be inverted surge tank and open the second peristaltic pump 7, during aqueous solution test sample pumped into collection bacterium bottle, when
Aqueous solution test sample is transferred to the second peristaltic pump 7 of rear closing in collection bacterium bottle completely；
（9）Red cap stopper 12 is covered into collection bacterium top of bottle, the second peristaltic pump 7 is opened after just putting surge tank, gas is pumped into collection bacterium bottle
In be pressurized, by collect bacterium bottle in miillpore filter to aqueous solution test sample filter and microorganism be enriched with, when the aqueous solution for try
Product close the second peristaltic pump 7 from after collection bacterium bottle discharge completely, remove red cap stopper 12 to fixed position；
（10）Syringe needle on surge tank is extracted and inserted on cleaning fluid bottle, cleaning fluid bottle is inverted and is opened the second peristaltic pump 7, will
Cleaning fluid is pumped into collection bacterium bottle, and the second peristaltic pump 7 is closed after cleaning fluid reaches certain capacity；
（11）Red cap stopper 12 is covered into collection bacterium top of bottle, the second peristaltic pump 7 is opened after just putting cleaning fluid bottle, gas is pumped into collection bacterium
It is pressurized in bottle, cleaning fluid discharges the cleaning realized to miillpore filter from collection bacterium bottle, is closed after cleaning fluid is completely exhausted out
Second peristaltic pump 7, removes red cap stopper 12 to fixed position；
（12）Yellow cap stopper 11 is moved into the bottom of collection bacterium bottle and is pressed into, sealed, prevent water clock；
（13）Syringe needle on cleaning fluid bottle is extracted and inserted on the medium bottle equipped with a certain culture medium, is simultaneously closed off and the
2nd, after the flexible pipe that the 3rd collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into first
In collection bacterium bottle, the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
（14）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with same medium, simultaneously close off with
Firstth, after the flexible pipe that the 3rd collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into second
In individual collection bacterium bottle, the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
（15）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with different culture media, simultaneously close off with
After the flexible pipe that first, second collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into the 3rd
In individual collection bacterium bottle, the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
（16）Red cap stopper 12 is covered into collection bacterium top of bottle, three flexible pipes being connected with collection bacterium bottle are cut and seal simultaneously.
（17）Surge tank/profit film liquid/cleaning fluid/medium bottle puts back to surge tank/profit film liquid/cleaning fluid/medium bottle box 6
It is interior, while the flexible pipe of the first peristaltic pump 5 and the upper band syringe needle of the second peristaltic pump 7 is removed, all of discarded object is transferred to flexible pipe
The like waste recovery area 14.
（18）Reset all devices to original state, waits the automatic collection bacterium operation of next batch aqueous solution test sample.
The Multi-freedom-degreemanipulator manipulator coordinated of use is operated：
First manipulator 1 carries out cooperating with ampoule bottle opener 25：Peace in the gripping ampoule bottle box 2 of first manipulator 1
Small jar bottle, and place into ampoule bottle opener 25；Combine with ampoule bottle opener 25 carry out ampoule bottle cutting, fracture behaviour
Make, realize that ampoule bottle is opened；After ampoule bottle is opened, the first manipulator 1 puts back in ampoule bottle box 2 ampoule bottle；Treat in ampoule bottle
Aqueous solution test sample be transferred to surge tank after, ampoule bottle discarded object is transferred to the recovery areas such as reagent bottle 22 by the first manipulator 1.
Second manipulator 4 and the 6th manipulator 17 carry out cooperating：Second manipulator 4 gripping surge tank, profit film liquid bottle,
Cleaning fluid bottle or medium bottle, and be put into surge tank/profit film liquid/cleaning fluid/medium bottle box 6；6th manipulator 17 is gripped
The syringe needle of the flexible pipe one end being connected with collection bacterium bottle, and insert the needle into or extract surge tank, moisten film liquid bottle, cleaning fluid bottle or culture
Base bottle, the second manipulator 4 overturns to surge tank, profit film liquid bottle, cleaning fluid bottle or medium bottle；When surge tank, profit film liquid
Bottle, cleaning fluid bottle or medium bottle in solution be transferred to collection bacterium bottle after, the second manipulator 4 by surge tank, profit film liquid bottle, cleaning
Placed to the recovery areas such as reagent bottle 22 in liquid bottle or medium bottle.
5th manipulator 15, the 6th manipulator 17 and the second flexible pipe install auxiliary body 16 and carry out cooperating：6th machine
Tool hand 17 grips the syringe needle of the flexible pipe one end being connected with collection bacterium bottle, the gripping collection bacterium bottle of the 5th manipulator 15, while stretching and movement
Flexible pipe is put it into the second peristaltic pump 7, and the second flexible pipe installation auxiliary body 16 is secured the hose in the second peristaltic pump 7.
5th manipulator 15, flexible pipe current limliting/manipulator 9 of heat sealing mechanism 8 and the 3rd carries out cooperating：5th manipulator 15
Gripping collection bacterium bottle simultaneously stretches the flexible pipe being connected with collection bacterium bottle, and the 3rd manipulator 9 is by flexible pipe press-in flexible pipe current limliting/heat sealing mechanism 8；
After flexible pipe is loaded into flexible pipe current limliting/heat sealing mechanism 8, the 5th manipulator 15 will collect bacterium bottle and be put into collection bacterium bottle rack 10.When being trained
Support during group-transfer to collection bacterium bottle, flexible pipe current limliting/heat sealing mechanism 8 carries out selective current limliting to flexible pipe, by media transfer extremely
In corresponding bacteria gathering device；After media transfer to collection bacterium bottle, flexible pipe current limliting/heat sealing mechanism 8 will cut off and collect what bacterium bottle was connected
Flexible pipe, and heated or pinch seal.
5th manipulator 15, the 6th manipulator 17, flexible pipe current limliting/heat sealing mechanism 8 carry out cooperateing with behaviour with the 3rd manipulator 9
Make：After the flexible pipe that flexible pipe current limliting/cut-out of heat sealing mechanism 8 is connected with collection bacterium bottle, the flexible pipe after the gripping cut-out of the 6th manipulator 17
Syringe needle, flexible pipe is transferred to flexible pipe the like waste recovery area 14.
7th manipulator 19, the 8th manipulator 20 and the first flexible pipe install auxiliary body 3 and carry out cooperating；7th machinery
The syringe needle of the gripping flexible pipe of hand 19 one end, the 8th manipulator 20 grips the syringe needle of the flexible pipe other end, while stretching and mobile flexible pipe will
It is put into the first peristaltic pump 5, and the first flexible pipe installation auxiliary body 3 is secured the hose in the first peristaltic pump 5.
7th manipulator 19, the 8th manipulator 20 and the second manipulator 4 carry out cooperating：Second manipulator 4 clamps slow
Tank is rushed, the syringe needle of the gripping flexible pipe of the 7th manipulator 19 one end, movement simultaneously inserts surge tank；The gripping flexible pipe of 8th manipulator 20 is another
The syringe needle at end, movement simultaneously inserts ampoule bottle, the aqueous solution test sample in ampoule bottle is transferred in surge tank, in current ampoule bottle
The transfer of aqueous solution test sample finish after, during the 8th manipulator 20 extracts syringe needle, movement and inserts the ampoule bottle that other have turned on
The transfer of aqueous solution test sample is carried out, the aqueous solution test sample repeated to whole ampoule bottles is transferred in surge tank；Treat
After aqueous solution test sample in whole ampoule bottles is transferred in surge tank, the 7th manipulator 19 extracts the syringe needle of flexible pipe one end,
8th manipulator 20 extracts the syringe needle of the flexible pipe other end, and flexible pipe discarded object is transferred to flexible pipe the like waste by the 8th manipulator 20
Recovery area 14.
8th manipulator 20 and the first peristaltic pump 5 carry out cooperating：When the 8th manipulator 20 is the syringe needle of flexible pipe one end
After insertion ampoule bottle, the first peristaltic pump 5 is opened, carry out aqueous solution test sample transfer；When the aqueous solution in current ampoule bottle is for examination
After product are transferred to surge tank, the first peristaltic pump 5 is closed；8th manipulator 20 pulls out the syringe needle of flexible pipe one end from current ampoule bottle
Go out and insert other and have turned on ampoule bottle, open the first peristaltic pump 5, carry out the transfer of aqueous solution test sample；Circulate operation until
Aqueous solution test sample in all ampoule bottles is fully transferred in surge tank.
4th manipulator 13, the second peristaltic pump 7 and the second manipulator 4 carry out cooperating：4th manipulator 13 will be from collection
Yellow cap stopper 11, red cap stopper 12 are taken out in bacterium bottle/flexible pipe/cap stopper box 18, and is placed to fixed position；When carrying out profit film liquid, water-soluble
When liquid test sample and cleaning fluid are transferred to collection bacterium bottle, the second manipulator 4 is inverted surge tank, profit film liquid bottle or cleaning fluid bottle, opens
Second peristaltic pump 7；After completion profit film liquid, aqueous solution test sample and cleaning fluid are transferred to collection bacterium bottle, the second peristaltic pump 7 is closed,
4th manipulator 13 will cover red cap stopper 12 to collection bacterium bottle；After red cap stopper 12 is covered, the second manipulator 4 just putting surge tank,
Profit film liquid bottle or cleaning fluid bottle or medium bottle, open the second peristaltic pump 7；When profit film liquid, aqueous solution test sample and cleaning fluid from
After collection bacterium bottle discharge, the second peristaltic pump 7 is closed, the 4th manipulator 13 removes to fixed position red cap stopper 12 from collection bacterium bottle；
After the completion of collection bacterium bottle cleaning, the 4th manipulator 13 is sealed the press-in collection bacterium bottom of bottle portion of yellow cap stopper, and the second manipulator 4 falls
Medium bottle is put, the second peristaltic pump 7 is opened；After three bottles of culture mediums are fully transferred to collection bacterium bottle, the second peristaltic pump 7 is closed, the
Four manipulators 13 will cover red cap stopper 12 to collection bacterium bottle.
Therefore each manipulator degrees-of-freedom demand in automation operating system is：First manipulator 1 needs three translations certainly
By spending, the second manipulator 4 needs three translation freedoms, a rotary freedoms, and one translation of the needs of the 3rd manipulator 9 is freely
Degree, the 4th manipulator 13, the 5th manipulator 15, the 6th manipulator 17, the 7th manipulator 19 and the 8th manipulator 20 are required to three
Individual translation freedoms.
Claims (6)
1. towards the full-automatic streamline collection bacterium operating method of the bottled aqueous solution test sample of ampoule, it is characterised in that use string,
Parallel processing and flow optimization method, comprise the following steps：
Step（1）Ampoule bottle box (2), surge tank/profit film liquid/cleaning fluid/medium bottle box (6), collection bacterium bottle on gripping guide rail/
Flexible pipe/cap stopper box (18) is respectively put into fixed position；
Step（2）Collection bacterium bottle/flexible pipe/cap stopper box (18) is opened, the flexible pipe with syringe needle being connected with collection bacterium bottle is installed into second
Peristaltic pump (7), while the another set of flexible pipe with syringe needle in box is installed into the first peristaltic pump（5）；
Step（3）The flexible pipe with syringe needle being connected with collection bacterium bottle is installed into flexible pipe current limliting/heat sealing mechanism (8)；Bacterium will be collected simultaneously
Bottle is put into collection bacterium bottle rack (10), and the yellow cap stopper (11), red cap stopper (12) in collection bacterium bottle/flexible pipe/cap stopper box (18) are put into solid
Positioning is put；
Step（4）Syringe needle on the flexible pipe in the second peristaltic pump (7) is inserted into surge tank/profit film liquid/cleaning fluid/culture
On profit film liquid bottle in base bottle box (6), the second peristaltic pump (7) is opened after being inverted profit film liquid bottle, will be during profit film liquid pumps into collection bacterium bottle
Miillpore filter is soaked, the second peristaltic pump (7) is closed after profit film liquid reaches certain capacity；
Step（5）Red cap stopper (12) is covered into collection bacterium top of bottle, the second peristaltic pump is opened after just putting profit film liquid bottle, gas is pumped into
It is pressurized in collection bacterium bottle so that profit film liquid from collection bacterium bottle discharge, the second peristaltic pump is closed after profit film liquid is completely exhausted out completely,
Remove red cap stopper to fixed position；
Step（6）In step（2）To step（5）While carrying out, the ampoule bottle in ampoule bottle box is sent to ampoule bottle and opens machine
Structure（21）In opened, during unlatching sends back to ampoule bottle box after terminating；
Step（7）In step（6）While carrying out, will be installed in the first peristaltic pump（5）In flexible pipe one end on syringe needle insertion
In the ampoule bottle having turned on, the buffering in the syringe needle insertion surge tank/profit film liquid/cleaning fluid/medium bottle box of the flexible pipe other end
On tank, the first peristaltic pump of unlatching pumps into the aqueous solution test sample in ampoule bottle in surge tank；Treat that the aqueous solution in ampoule bottle is supplied
After test product pumps into surge tank completely, the first peristaltic pump is closed；Syringe needle is removed the peace that the ampoule bottle for having finished has turned on to other
In small jar bottle, the first peristaltic pump is opened（5）During aqueous solution test sample in ampoule bottle pumped into surge tank；
Step（8）After the aqueous solution test sample in the ampoule bottle of all feedings pumps into surge tank completely, by profit film liquid bottle
Syringe needle is extracted and inserted on surge tank, is inverted surge tank and is opened the second peristaltic pump, during aqueous solution test sample pumped into collection bacterium bottle,
The second peristaltic pump is closed after aqueous solution test sample is transferred in collection bacterium bottle completely；
Step（9）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting surge tank, gas is pumped into collection bacterium bottle
In be pressurized, by collect bacterium bottle in miillpore filter to aqueous solution test sample filter and microorganism be enriched with, when the aqueous solution for try
Product close the second peristaltic pump from after collection bacterium bottle discharge completely, remove red cap stopper to fixed position；
Step（10）Syringe needle on surge tank is extracted and inserted on cleaning fluid bottle, cleaning fluid bottle is inverted and is opened second and wriggle
Pump, by cleaning fluid pump into collection bacterium bottle in, the second peristaltic pump is closed after cleaning fluid reaches certain capacity；
Step（11）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting cleaning fluid bottle, gas is pumped into collection
It is pressurized in bacterium bottle, cleaning fluid discharges the cleaning realized to miillpore filter from collection bacterium bottle, is closed after cleaning fluid is completely exhausted out
The second peristaltic pump is closed, red cap stopper to fixed position is removed；
Step（12）By yellow cap stopper（11）Move to the bottom of collection bacterium bottle and be pressed into, sealed, prevent water clock；
Step（13）By cleaning fluid bottle on syringe needle extract and insert on the medium bottle equipped with a certain culture medium, simultaneously close off with
After the flexible pipe that second, third collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump, culture medium is pumped into first
In collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity；
Step（14）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with same medium, while closing
Close with after the first, the 3rd collection bacterium flexible pipe that is connected of bottle, be inverted medium bottle and the second peristaltic pump of unlatching, culture medium is pumped into the
In two collection bacterium bottles, the second peristaltic pump is closed after culture medium reaches certain capacity；
Step（15）Syringe needle on medium bottle is extracted and inserted on another medium bottle equipped with different culture media, while closing
Close with after the first, second collection bacterium flexible pipe that is connected of bottle, be inverted medium bottle and the second peristaltic pump of unlatching, culture medium is pumped into the
In three collection bacterium bottles, the second peristaltic pump is closed after culture medium reaches certain capacity；
Step（16）Red cap stopper lid is entered to collect bacterium top of bottle, three flexible pipes being connected with collection bacterium bottle are cut and seal simultaneously；
Step（17）Surge tank/profit film liquid/cleaning fluid/medium bottle puts back to surge tank/profit film liquid/cleaning fluid/medium bottle box
（6）It is interior, while the flexible pipe of the first peristaltic pump and the second peristaltic pump upper band syringe needle is removed, all of discarded object is transferred to flexible pipe
The like waste recovery area（14）；
Step（18）Reset all devices to original state, waits the automatic collection bacterium operation of next batch aqueous solution test sample.
2. the full-automatic streamline collection bacterium operation side towards the bottled aqueous solution test sample of ampoule according to claim 1
Method, it is characterised in that：Ampoule bottle box（2）The opening time of interior whole ampoule bottles is transferred to buffering with whole aqueous solution test samples
The time sum of tank, should be less than being equal to peace of the flexible pipe being connected with collection bacterium bottle in the second peristaltic pump, flexible pipe current limliting/heat sealing mechanism
ETL estimated time of loading and the wetting, the efflux time sum that collect miillpore filter in bacterium bottle.
3. the full-automatic streamline collection bacterium operation side towards the bottled aqueous solution test sample of ampoule according to claim 1
Method, it is characterised in that：Aqueous solution test sample species to be checked need to meet requirement to the flushing dose of cleaning fluid, if flushing dose is super
Single bottle cleaning fluid is crossed, it is necessary in step（11）Increase by two steps afterwards：
Step（a）Syringe needle on first cleaning fluid bottle is extracted and inserted on the second cleaning fluid bottle, the second cleaning fluid bottle is inverted simultaneously
Open the second peristaltic pump by cleaning fluid pump into collection bacterium bottle in, the second peristaltic pump is closed after cleaning fluid reaches certain capacity；
Step（b）Red cap stopper lid is entered to collect bacterium top of bottle, the second peristaltic pump is opened after just putting the second cleaning fluid bottle, gas is pumped into
It is pressurized in collection bacterium bottle, cleaning fluid discharges the cleaning realized to miillpore filter from collection bacterium bottle, after cleaning fluid is completely exhausted out
The second peristaltic pump is closed, red cap stopper to fixed position is removed.
4. towards the full-automatic streamline collection bacterium operating system of the bottled aqueous solution test sample of ampoule, including the first manipulator（1）、
Ampoule bottle box（2）, the first flexible pipe install auxiliary body（3）, the second manipulator（4）, the first peristaltic pump（5）, surge tank/profit film
Liquid/cleaning fluid/medium bottle box（6）, the second peristaltic pump（7）, flexible pipe current limliting/heat sealing mechanism（8）, the 3rd manipulator（9）, collection bacterium
Bottle rack（10）, yellow cap stopper（11）, red cap stopper（12）, the 4th manipulator（13）, flexible pipe the like waste recovery area（14）, the 5th machine
Tool hand（15）, the second flexible pipe install auxiliary body（16）, the 6th manipulator（17）, collection bacterium bottle/flexible pipe/cap stopper box（18）, the 7th
Manipulator（19）, the 8th manipulator（20）, ampoule bottle opener（21）, the recovery area such as reagent bottle（22）, it is characterised in that：
First manipulator（1）With ampoule bottle opener（21）Carry out cooperating：First manipulator（1）Take ampoule bottle box
（2）Interior ampoule bottle, and place into ampoule bottle opener；Combining with ampoule bottle opener carries out ampoule bottle cutting, folding
Disconnected operation, realizes that ampoule bottle is opened；After ampoule bottle is opened, the first manipulator puts back in ampoule bottle box ampoule bottle；Treat ampoule bottle
After interior aqueous solution test sample is transferred to surge tank, ampoule bottle discarded object is transferred to the recovery areas such as reagent bottle by the first manipulator
（22）；
Second manipulator（4）With the 6th manipulator（17）Carry out cooperating：Second manipulator gripping surge tank, profit film liquid
Bottle, cleaning fluid bottle or medium bottle, and it is put into surge tank/profit film liquid/cleaning fluid/medium bottle box（6）In；6th manipulator
（17）The syringe needle of the flexible pipe one end being connected with collection bacterium bottle is gripped, and inserted the needle into or is extracted surge tank, moisten film liquid bottle, cleaning fluid
Bottle or medium bottle, the second manipulator overturn to surge tank, profit film liquid bottle, cleaning fluid bottle or medium bottle；When surge tank,
Profit film liquid bottle, cleaning fluid bottle or medium bottle in solution be transferred to collection bacterium bottle after, the second manipulator by surge tank, profit film liquid
Placed to recovery areas such as reagent bottles in bottle, cleaning fluid bottle or medium bottle；
5th manipulator（15）, the 6th manipulator（17）Auxiliary body is installed with the second flexible pipe（16）Carry out cooperating：
6th manipulator grips the syringe needle of the flexible pipe one end being connected with collection bacterium bottle, the 5th manipulator（15）Gripping collection bacterium bottle, while stretching
The second peristaltic pump is put it into mobile flexible pipe（7）In, the second flexible pipe installs auxiliary body and secures the hose to the second peristaltic pump
In；
5th manipulator, flexible pipe current limliting/heat sealing mechanism（8）With the 3rd manipulator（9）Carry out cooperating：5th manipulator
Gripping collection bacterium bottle simultaneously stretches the flexible pipe being connected with collection bacterium bottle, and the 3rd manipulator is by flexible pipe press-in flexible pipe current limliting/heat sealing mechanism；Will
After flexible pipe loads flexible pipe current limliting/heat sealing mechanism, the 5th manipulator will collect bacterium bottle and be put into collection bacterium bottle rack（10）；When carrying out culture medium
It is transferred to during collection bacterium bottle, flexible pipe current limliting/heat sealing mechanism carries out selective current limliting to flexible pipe, by media transfer to corresponding
In bacteria gathering device；After media transfer to collection bacterium bottle, the flexible pipe that flexible pipe current limliting/heat sealing mechanism will cut off and collection bacterium bottle is connected, and
Heated or pinch seal；
5th manipulator, the 6th manipulator, flexible pipe current limliting/heat sealing mechanism and the 3rd manipulator（9）Carry out cooperating：When
After the flexible pipe that flexible pipe current limliting/heat sealing mechanism cut-out is connected with collection bacterium bottle, the syringe needle of the flexible pipe after the gripping cut-out of the 6th manipulator will
Flexible pipe is transferred to flexible pipe the like waste recovery area（14）；
7th manipulator（19）, the 8th manipulator（20）Auxiliary body is installed with the first flexible pipe（3）Carry out cooperating；The
The syringe needle of seven manipulators gripping flexible pipe one end, the 8th manipulator grips the syringe needle of the flexible pipe other end, while stretching and mobile flexible pipe
Put it into the first peristaltic pump, the first flexible pipe installation auxiliary body is secured the hose in the first peristaltic pump；
7th manipulator, the 8th manipulator and the second manipulator carry out cooperating：Second manipulator clamps surge tank, the
The syringe needle of seven manipulators gripping flexible pipe one end, movement simultaneously inserts surge tank；8th manipulator grips the syringe needle of the flexible pipe other end, moves
Ampoule bottle is moved and inserted, the aqueous solution test sample in ampoule bottle is transferred in surge tank, the aqueous solution in current ampoule bottle is supplied
After test product transfer is finished, the 8th manipulator is extracted syringe needle, movement and is inserted and carry out aqueous solution confession in the ampoule bottle that other have turned on
The transfer of test product, the aqueous solution test sample repeated to whole ampoule bottles is transferred in surge tank；Treat in whole ampoule bottles
Aqueous solution test sample be transferred in surge tank after, the 7th manipulator extracts the syringe needle of flexible pipe one end, and the 8th manipulator will be soft
The syringe needle of the pipe other end is extracted, and flexible pipe discarded object is transferred to flexible pipe the like waste recovery area by the 8th manipulator；
8th machinery carries out cooperating with the first peristaltic pump：When the syringe needle of flexible pipe one end is inserted ampoule by the 8th manipulator
After bottle, the first peristaltic pump is opened, carry out aqueous solution test sample transfer；Aqueous solution test sample in current ampoule bottle is transferred to buffering
After tank, the first peristaltic pump is closed；8th manipulator extracts the syringe needle of flexible pipe one end from current ampoule bottle and inserts other
Ampoule bottle is opened, the first peristaltic pump is opened, the transfer of aqueous solution test sample is carried out；Circulate operation is up to the water in all ampoule bottles
Solution test sample is fully transferred in surge tank；
4th manipulator, the second peristaltic pump and the second manipulator carry out cooperating：4th manipulator will from collection bacterium bottle/it is soft
Yellow cap stopper, red cap stopper are taken out in pipe/cap stopper box, and is placed to fixed position；When carrying out profit film liquid, aqueous solution test sample and cleaning
When liquid is transferred to collection bacterium bottle, the second manipulator is inverted surge tank, profit film liquid bottle or cleaning fluid bottle, opens the second peristaltic pump；When complete
After being transferred to collection bacterium bottle into profit film liquid, aqueous solution test sample and cleaning fluid, the second peristaltic pump is closed, the 4th manipulator will be covered into red
On cap stopper to collection bacterium bottle；After red cap stopper is covered, the second manipulator is just putting surge tank, profit film liquid bottle or cleaning fluid bottle or culture
Base bottle, opens the second peristaltic pump；After film liquid, aqueous solution test sample and cleaning fluid is moistened from collection bacterium bottle discharge, close second and wriggle
Pump, the 4th manipulator removes to fixed position red cap stopper from collection bacterium bottle；After the completion of collection bacterium bottle cleaning, the 4th manipulator will
The press-in collection bacterium bottom of bottle portion of yellow cap stopper is sealed, and the second manipulator is inverted medium bottle, opens the second peristaltic pump；When three bottles of trainings
After foster base is fully transferred to collection bacterium bottle, the second peristaltic pump is closed, the 4th manipulator will cover red cap stopper to collection bacterium bottle.
5. the full-automatic streamline collection bacterium towards the bottled aqueous solution test sample of ampoule according to claim 5 operates system
System, it is characterised in that：Collection bacterium bottle is capable of achieving filtering and the Enrichment of bacteria of aqueous solution test sample built with miillpore filter.
6. the full-automatic streamline collection bacterium towards the bottled aqueous solution test sample of ampoule according to claim 5 operates system
System, it is characterised in that：Manipulator is the shifting with identical function grabbed with multivariant mechanical arm or with manipulator
Motivation structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710029497.3A CN106834422B (en) | 2017-01-16 | 2017-01-16 | Full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710029497.3A CN106834422B (en) | 2017-01-16 | 2017-01-16 | Full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106834422A true CN106834422A (en) | 2017-06-13 |
| CN106834422B CN106834422B (en) | 2019-07-19 |
Family
ID=59123123
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710029497.3A Active CN106834422B (en) | 2017-01-16 | 2017-01-16 | Full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106834422B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113549540A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria collecting system |
| CN113549534A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria-collecting operation system's control infusion and bacteria-collecting device |
| CN113549534B (en) * | 2021-06-29 | 2024-05-31 | 杭州电子科技大学 | Intelligent bacteria collecting operation system's control infusion and bacteria collecting device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2237047Y (en) * | 1995-02-22 | 1996-10-09 | 叶大林 | Group bacteria cultivating device |
| CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
| CN201756550U (en) * | 2010-06-29 | 2011-03-09 | 中国人民解放军第三○二医院 | Fully-sealed bacteria collection ampoule incubator |
| CN106226151A (en) * | 2016-07-22 | 2016-12-14 | 牛刚 | Aseptic cabin automatic medicine sterility test enrichment system and operational approach thereof |
-
2017
- 2017-01-16 CN CN201710029497.3A patent/CN106834422B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2237047Y (en) * | 1995-02-22 | 1996-10-09 | 叶大林 | Group bacteria cultivating device |
| CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
| CN201756550U (en) * | 2010-06-29 | 2011-03-09 | 中国人民解放军第三○二医院 | Fully-sealed bacteria collection ampoule incubator |
| CN106226151A (en) * | 2016-07-22 | 2016-12-14 | 牛刚 | Aseptic cabin automatic medicine sterility test enrichment system and operational approach thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周晓等: "智能集菌仪无菌检查法在灭菌制剂中的应用", 《中国医院药学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113549540A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria collecting system |
| CN113549534A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria-collecting operation system's control infusion and bacteria-collecting device |
| CN113549534B (en) * | 2021-06-29 | 2024-05-31 | 杭州电子科技大学 | Intelligent bacteria collecting operation system's control infusion and bacteria collecting device |
| CN113549540B (en) * | 2021-06-29 | 2024-05-31 | 杭州电子科技大学 | Intelligent bacteria collecting system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106834422B (en) | 2019-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105670916B (en) | Aseptic packaging interlayer sterility test totally-enclosed membrane filter and its detection method | |
| CN106834421B (en) | Full-automatic assembly line collection bacterium operating method and system towards the bottled powder test sample in XiLin | |
| CN106226151B (en) | Sterile cabin automatic medicine sterility test enrichment system and its operating method | |
| CN104248512B (en) | Ampoule bottle dosage and check valve-type dispensation apparatus | |
| CN103025857A (en) | Automated filling of flexible cryogenic storage bags with therapeutic cells | |
| CN106834422A (en) | Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule | |
| CN202497449U (en) | Three-combination bag used for collection, separation and preservation of umbilical cord blood | |
| CN2605006Y (en) | Disposable blood-taking bag | |
| CN207623067U (en) | Blood bag blood pigtail disposably takes blood casing | |
| CN207575482U (en) | A kind of injector for medical purpose for facilitating drawing liquid | |
| CN203159612U (en) | Centrifuge tube structure for extracting DNA (deoxyribonucleic acid) by adopting silicon bead method | |
| CN206597162U (en) | Blood bag for cord blood collection | |
| CN202446542U (en) | Multifunctional blood sampling and transfusing device | |
| CN102430161B (en) | Multifunctional blood collection | |
| CN207267876U (en) | Pipeline for cell injuring model | |
| CN204474691U (en) | Cell culture bags special filling liquid funnel | |
| CN203148726U (en) | Sterile sampling bottle | |
| CN206285115U (en) | Practical blood gas analysis hemostix | |
| CN205821337U (en) | A kind of aseptic cabin pharmaceutical sterile test enrichment Operation Van | |
| CN207659446U (en) | A kind of candidate stem cell acquisition extraction three joint bag | |
| CN206940863U (en) | A kind of 24 hole treatments of the sample plates of automatic separation of solid and liquid | |
| CN205861441U (en) | Pharmaceutical sterile test enrichment parallel-type filter | |
| CN204543096U (en) | A kind of platelet transfusion device | |
| CN2392549Y (en) | Suction device for hydrops in cavity of humanbody | |
| CN219567905U (en) | Microorganism sampling bag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |