CN203148726U - Sterile sampling bottle - Google Patents
Sterile sampling bottle Download PDFInfo
- Publication number
- CN203148726U CN203148726U CN 201320111721 CN201320111721U CN203148726U CN 203148726 U CN203148726 U CN 203148726U CN 201320111721 CN201320111721 CN 201320111721 CN 201320111721 U CN201320111721 U CN 201320111721U CN 203148726 U CN203148726 U CN 203148726U
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- sampling
- bottle
- valve
- pipe
- sterile sampling
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- Sampling And Sample Adjustment (AREA)
Abstract
The utility model is suitable for the field of sterile sampling devices and provides a sterile sampling bottle which comprises a container bottle and a bottle cap, and also comprises a sampling pipe and a breathing pipe one ends of which penetrate through the bottle cap and are inserted into the container bottle respectively, the sampling pipe and the breathing pipe are in sealing connection to the bottle cap, a microporous filter membrane filter is connected to the other end of the breathing pipe, the other end of the sampling pipe is connected with a sampling valve, the other end of the sampling valve is connected with a t-branch pipe, one branch of the t-branch pipe is connected with a feeding valve, another branch of the t-branch pipe is connected with a drain valve, and the other end of the feeding valve is connected with a sampling chuck. The sampling pipe and the breathing pipe are arranged and the microporous filter membrane filter is connected to the breathing pipe, and thus infectious microbes are prevented from entering the container bottle through the breathing pipe. The t-branch pipe is connected to the sampling pipe, the sampling valve, the feeding valve and the drain valve are respectively connected to three branches of the t-branch pipe, the other end of the feeding valve is connected with the sampling chuck, and thus the container bottle, the t-branch pipe and the sampling chuck are firstly sterilized and disinfected during the sampling process, thereby ensuring that infectious microbes cannot be introduced in the obtained sample.
Description
Technical field
The utility model belongs to the sterile sampling device field, relates in particular to the sterile sampling bottle.
Background technology
At present, mostly sterile sampling bottle commonly used is the closed aseptic sampling jar that sterilization material such as built-in sodium thiosulfate constitutes, and because containing sterilization material, the time spent can directly be carried out sampling operation.Open the steam passage of the fermenter equipment sample tap of waiting to take a sample earlier, to equipment sample tap steam sterilizing, steam off path is more directly opened the sampling path of bottle cap and fermenter equipment, takes a sample, and covers bottle cap after having got immediately.But, when opening bottle cap, be easy to introduce assorted bacterium, influence the judgement of thalline state in the sample.
The utility model content
The purpose of this utility model is to provide a kind of sterile sampling bottle, easily introduces the problem of assorted bacterium when being intended to solve current sterile sampling bottle sampling.
The utility model is to realize like this, a kind of sterile sampling bottle, comprise the container Bottle and bottle cap, also comprise and respectively have an end to pass described bottle cap, insert stopple coupon and respiratory siphon in the described container bottle, described stopple coupon and described respiratory siphon are sealedly attached on the described bottle cap, the other end of described respiratory siphon is connected to nuclepore membrane filter, the other end of described stopple coupon connects sample valve, another termination three-way pipe of described sample valve, described three-way pipe one branch road connects sampling valve, another branch road connects drain valve, and the other end of described sampling valve accesses the scale dish.
Further, also be provided with the connector with through hole on the described bottle cap, described stopple coupon and described respiratory siphon insert in the described through hole and are sealed on the described connector.
Particularly, described sampling valve is made up of first flexible pipe and first hose clamp that is held on described first flexible pipe.
Particularly, described drain valve is made up of second flexible pipe and second hose clamp that is held on described second flexible pipe.
Further, another termination the 3rd flexible pipe of described nuclepore membrane filter.
Further, the other end of described drain valve connects waste pipe.
Particularly, described sampling chuck is infundibulate, and its top end diameter scope is 5～10 centimetres.
Particularly, the micropore size scope of described nuclepore membrane filter is 0.05 μ m～2 μ m.
Particularly, an end of described stopple coupon inserts described container bottle bottom.
Particularly, an end of described respiratory siphon inserts described container finish place.
The utility model is by arranging stopple coupon and respiratory siphon, and connects nuclepore membrane filter at respiratory siphon and enter container bottle with antiforeign bacteria through respiratory siphon.Connect three-way pipe at stopple coupon, three-way pipe three the tunnel on connect sample valve, sampling valve and drain valve respectively, and access the scale dish at the other end of sampling valve, thus can be when sampling, earlier container bottle, three-way pipe and sampling chuck are carried out sterilization, the sample that assurance obtains can not introduced assorted bacterium.
Description of drawings
Fig. 1 is the structural representation of a kind of sterile sampling bottle of providing of the utility model.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explaining the utility model, and be not used in restriction the utility model.
See also Fig. 1, a kind of sterile sampling bottle that the utility model embodiment provides comprises container bottle 1 and bottle cap 2, at bottle cap 2 two pipelines is set, and one is sampling line, and another is for breathing pipeline.Sampling line comprises that an end passes the stopple coupon 7 that bottle cap 2 inserts in the container bottle 1, and stopple coupon 7 is sealed on the bottle cap 2, the other end of stopple coupon 7 connects sample valve 8, another termination three-way pipe 9 of sample valve 8, three-way pipe 9 one branch roads connect sampling valve 10, another branch road connects drain valve 11, and the other end of sampling valve 10 accesses scale dish 12.Breathe pipeline and comprise that an end passes the respiratory siphon 4 that bottle cap 2 inserts in the container bottle 1, and respiratory siphon 4 is sealed on the bottle cap 2, the other end of respiratory siphon 4 is connected to nuclepore membrane filter 5.
Before use, close sample valve 8, more whole sterile sampling bottle is put into autoclave, in about 121 ℃ of moist heat sterilization 20min.During use sampling chuck 12 is connected on and waits the sample connection place of fermenter equipment of taking a sample, and add the silica gel sealing pad, seal with the sample connection chucking with the clamp chuck 12 of will take a sample then.Close drain valve 11, open the steam passage of fermenter equipment sample tap again, open sampling valve 10, open drain valve 11 condensate water of draining, and sampling chuck 12 is carried out steam sterilizing; Close the steam passage of fermenter equipment sample tap then, close drain valve 11, open the sampling path of sample valve 8 and fermenter equipment, can take a sample.
Because before the sampling, carried out sterilization, and before sampling, sample valve 8 cuts out, and has guaranteed container bottle 1 and breathed pipeline aseptic, be connected to nuclepore membrane filter 5 on the respiratory siphon 4, identical with the external world to guarantee the air pressure in the container bottle 1, and prevent that extraneous assorted bacterium from entering container bottle 1, then, also will be through steam sterilizing, to sterilizing in sampling chuck 12 and the three-way pipe 9; During sampling, drain valve 11 cuts out, and guarantees that sampling line is isolated from the outside, thereby when making sample enter container bottle 1, can not introduce assorted bacterium; Sampling is finished, and closes sample valve 8, makes extraneous assorted bacterium can not enter container bottle 1.Thereby can be safer, easy when guaranteeing sterile sampling take a sample.
Further, also be provided with the connector 3 with through hole on the bottle cap 2 of this sterile sampling bottle, show as described in Figure 1, in the present embodiment, connector 3 is located at the upper surface of bottle cap 2, and the upper end of its through hole is the flaring shape, and stopple coupon 7 and respiratory siphon 4 inserts in the through hole of connectors 3, and with its respectively with corresponding connector 3 sealings on.Can make things convenient for installation and the taking-up of stopple coupon 7 and respiratory siphon 4 like this.
Particularly, sampling valve 10 is made up of first flexible pipe and first hose clamp that is held on first flexible pipe.Drain valve 11 is made up of second flexible pipe and second hose clamp that is held on second flexible pipe.Certainly sample valve 8 also can be made up of flexible pipe and the hose clamp that is held on this flexible pipe.The break-make in each outlet on the three-way pipe 9 be can conveniently control like this, and flexible pipe and hose clamp used, when sampling, the convenient position of adjusting container bottle 1.Certainly among other embodiment, sample valve 8, sampling valve 10 and drain valve 11 also can be valves such as diaphragm valve, and each pipeline uses stereoplasm tube to connect, as metal tube etc.
Further, another termination the 3rd flexible pipe 6 of nuclepore membrane filter 5.Guide to the more position of cleaning in order to will breathe the pneumostome of pipeline, or control the break-make of breathing pipeline with hose clamp.
Further, the other end of drain valve 11 connects waste pipe 13.Thereby the steam during with steam sterilization and condensate water, and other material in the three-way pipe 9 etc. guides to positions such as extraneous discharging pond, in case contaminated environment.
Particularly, sampling chuck 12 is infundibulate, and its top end diameter scope is 5～10 centimetres, among other embodiment, also can be shapes such as cylindrical.The micropore size scope of nuclepore membrane filter 5 is 0.05 μ m～2 μ m.One end of stopple coupon 7 inserts container bottle 1 bottom, so that sample steadily enters container bottle 1.One end of respiratory siphon 4 inserts container bottle 1 bottle mouth position, and after preventing from adding sample, pressure of the inside of a bottle increases, and sample is extruded from respiratory siphon 4.
The sterile sampling bottle of present embodiment, reusable, and do not need to add other materials, save consumption.Stopple coupon 7 and respiratory siphon 4 adopt stainless-steel tube, the resistant to elevated temperatures vial of container bottle 1 usefulness or high silicon boron bottle.
The above only is preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of within spirit of the present utility model and principle, doing, be equal to and replace and improvement etc., all should be included within the protection domain of the present utility model.
Claims (10)
1. sterile sampling bottle, comprise the container Bottle and bottle cap, it is characterized in that: also comprise respectively having an end to pass described bottle cap, insert stopple coupon and respiratory siphon in the described container bottle, described stopple coupon and described respiratory siphon are sealedly attached on the described bottle cap, the other end of described respiratory siphon is connected to nuclepore membrane filter, the other end of described stopple coupon connects sample valve, another termination three-way pipe of described sample valve, described three-way pipe one branch road connects sampling valve, another branch road connects drain valve, and the other end of described sampling valve accesses the scale dish.
2. sterile sampling bottle as claimed in claim 1, it is characterized in that: also be provided with the connector with through hole on the described bottle cap, described stopple coupon and described respiratory siphon insert in the described through hole and are sealed on the described connector.
3. sterile sampling bottle as claimed in claim 1 is characterized in that: described sampling valve is made up of first flexible pipe and first hose clamp that is held on described first flexible pipe.
4. sterile sampling bottle as claimed in claim 1 is characterized in that: described drain valve is made up of second flexible pipe and second hose clamp that is held on described second flexible pipe.
5. sterile sampling bottle as claimed in claim 1 is characterized in that: another termination the 3rd flexible pipe of described nuclepore membrane filter.
6. sterile sampling bottle as claimed in claim 1, it is characterized in that: the other end of described drain valve connects waste pipe.
7. as each described sterile sampling bottle of claim 1-6, it is characterized in that: described sampling chuck is infundibulate, and its top end diameter scope is 5～10 centimetres.
8. as each described sterile sampling bottle of claim 1-6, it is characterized in that: the micropore size scope of described nuclepore membrane filter is 0.05 μ m～2 μ m.
9. as each described sterile sampling bottle of claim 1-6, it is characterized in that: an end of described stopple coupon inserts described container bottle bottom.
10. as each described sterile sampling bottle of claim 1-6, it is characterized in that: an end of described respiratory siphon inserts described container finish place.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320111721 CN203148726U (en) | 2013-03-12 | 2013-03-12 | Sterile sampling bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320111721 CN203148726U (en) | 2013-03-12 | 2013-03-12 | Sterile sampling bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203148726U true CN203148726U (en) | 2013-08-21 |
Family
ID=48976242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320111721 Expired - Lifetime CN203148726U (en) | 2013-03-12 | 2013-03-12 | Sterile sampling bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203148726U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| CN114166576A (en) * | 2021-11-22 | 2022-03-11 | 深圳市奥图威尔科技有限公司 | Closed sampling device capable of quantitatively sampling |
-
2013
- 2013-03-12 CN CN 201320111721 patent/CN203148726U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528302A (en) * | 2021-07-13 | 2021-10-22 | 深圳赛桥生物创新技术有限公司 | Quantitative, sterile and disposable automatic cell sampling device and method |
| CN114166576A (en) * | 2021-11-22 | 2022-03-11 | 深圳市奥图威尔科技有限公司 | Closed sampling device capable of quantitatively sampling |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 050000 No.16, East bangxiu Road, North Zone, Zhengding high tech Industrial Development Zone, Zhengding District, China (Hebei) pilot Free Trade Zone, Shijiazhuang City, Hebei Province Patentee after: Hebei Yiran Biotechnology Co.,Ltd. Address before: 050000 Yiran biological industrial park, Zhengding Economic Development Zone, Shijiazhuang City, Hebei Province Patentee before: HEBEI INATURAL BIOTECHNOLOGY Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130821 |