CN104059846B - A kind of culture device for anaerobic bacteria - Google Patents
A kind of culture device for anaerobic bacteria Download PDFInfo
- Publication number
- CN104059846B CN104059846B CN201410267957.2A CN201410267957A CN104059846B CN 104059846 B CN104059846 B CN 104059846B CN 201410267957 A CN201410267957 A CN 201410267957A CN 104059846 B CN104059846 B CN 104059846B
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- sealing
- conical flask
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- inlet pipe
- plug
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
- C12M43/06—Photobioreactors combined with devices or plants for gas production different from a bioreactor of fermenter
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Organic Chemistry (AREA)
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- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Treating Waste Gases (AREA)
Abstract
The invention discloses a kind of culture device for anaerobic bacteria, it is by carbon-dioxide generator, glass evacuated exsiccator and CO2 absorber system composition, described carbon-dioxide generator is a conical flask, equipped with calcium carbonate in conical flask, the upper end of conical flask is provided with sealing-plug, the upper end of sealing-plug is provided with separatory funnel, separatory funnel is built with hydrochloric acid, the upper end of separatory funnel is provided with gag, lower end is provided with switch, the lower end of switch is provided with leakage pipe, the lower end of leakage pipe is inserted in conical flask by the left portion of sealing-plug, the right side of conical flask is provided with glass evacuated exsiccator, glass evacuated dry right side is provided with CO2 absorber system, the upper end of CO2 absorber system is provided with sealing lid, the upper end sealing lid is provided with air inlet pipe, the exhaust connector connection of exhaustor is arranged on sealing and covers in the air inlet pipe of end.
Description
Technical field
The present invention relates to a kind of anaerobic culture equipment, be specifically a kind of laboratory simple microbiological anaerobic culture apparatus.
Background technology
Anaerobe is extensive in distributed in nature, of a great variety, and effect draws attention the most day by day.The key problem in technology cultivating anaerobe is to make this quasi-microorganism be in the environment of anoxia.Colleges and universities that strength is stronger or R&D institution; the basic skills carrying out anaerobic culture at laboratory is mainly Heng Shi anaerobism rolling tube technique, anaerobic jar technology, anaerobism glove box techniques, culture medium deoxygenation technology, airbag anaerobic system and anaerobic petri diss technology etc.; owing to the operation of these Anaerobic culturel methods is relatively complicated; equipment is costly; common laboratory does not satisfies the requirements, thus limits extensively carrying out of anaerobe research work.
Summary of the invention
It is an object of the invention to provide a kind of simple effective culture device for anaerobic bacteria, make full use of the conventional equipment in existing laboratory, engineered processing is developed into simple in construction, easy to operate, use safe culture device, to save resource, promote extensively carrying out of anaerobe research work.
The solution of the present invention is made up of carbon-dioxide generator, glass evacuated exsiccator and CO2 absorber system, described carbon-dioxide generator is a conical flask, equipped with calcium carbonate in conical flask, the upper end of conical flask is provided with sealing-plug, and the upper end of sealing-plug is provided with separatory funnel, and separatory funnel is built with hydrochloric acid, the upper end of separatory funnel is provided with gag, lower end is provided with switch, and the lower end of switch is provided with leakage pipe, and the lower end of leakage pipe is inserted in conical flask by the left portion of sealing-plug.nullThe right side of conical flask is provided with glass evacuated exsiccator，Glass evacuated dry right side is provided with CO2 absorber system，The upper end of glass evacuated exsiccator is provided with upper cover，The upper end of upper cover is provided with top pressure closure，The right middle of conical flask sealing-plug is provided with escape pipe，The right-hand member of escape pipe is provided with inlet pipe connection，The left end of inlet pipe connection is provided with air inlet union joint，The left end of inlet pipe connection is fastened on escape pipe by air inlet union joint，The right-hand member of inlet pipe connection is fixed on the top pressure closure of glass evacuated exsiccator upper end，The lower end of top pressure closure is provided with grass tube，The gas outlet of grass tube is arranged on the bottom of glass evacuated exsiccator，Oxygen concentration detection indicator it is provided with in glass evacuated exsiccator，The right middle of top pressure closure is provided with exhaustor，The right-hand member of exhaustor is provided with exhaust connector，The upper end of CO2 absorber system is provided with sealing lid，The upper end sealing lid is provided with air inlet pipe，The exhaust connector connection of exhaustor is arranged on sealing and covers in the air inlet pipe of end，CO2 absorber system is built with lime water.
During gas processed, open the switch of separatory funnel lower end, hydrochloric acid solution in funnel enters the generation carbon dioxide that reacts in conical flask with calcium carbonate, and by the bottom of inlet pipe connection and grass tube entrance glass evacuated exsiccator, air is discharged, CO2 absorber system is entered by exhaustor, during lime water muddiness in CO2 absorber system, then represent that carbon dioxide comes into exsiccator, when the oxygen detection indicator in exsiccator is become redness from blueness, i.e. represent that the oxygen concentration in exsiccator is 0.1%, inlet pipe connection and exhaustor is closed respectively with fixture, remove carbon-dioxide generator and absorber, glass evacuated exsiccator is moved into incubator.
Present configuration is simple, and reasonable in design, easy to install, processing cost is low.
It is described in further detail below in conjunction with the accompanying drawings.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram.
Detailed description of the invention
nullCarbon-dioxide generator 1 shown in Fig. 1 is a conical flask，The upper end of conical flask is provided with sealing-plug 2，The upper end of sealing-plug is provided with separatory funnel 5，The upper end of separatory funnel is provided with gag 6，The lower end of separatory funnel is provided with switch 4，The lower end of switch is provided with leakage pipe 3，The lower end of leakage pipe is inserted in conical flask by the left portion of sealing-plug，The right side of conical flask is provided with glass evacuated exsiccator 9，The right side of glass evacuated exsiccator is provided with CO2 absorber system 18，The upper end of glass evacuated exsiccator is provided with upper cover 14，The upper end of upper cover is provided with top pressure closure 12，The right middle of conical flask sealing-plug is provided with escape pipe 7，The right-hand member of escape pipe is provided with inlet pipe connection 11，The left end of inlet pipe connection is provided with air inlet union joint 8，The left end of inlet pipe connection is fastened on escape pipe by air inlet union joint，The right-hand member of inlet pipe connection is fixed on the top pressure closure of glass evacuated exsiccator upper end，The lower end of top pressure closure is provided with grass tube 10，The gas outlet of grass tube is arranged on the bottom of glass evacuated exsiccator，Oxygen concentration detection indicator 15 it is provided with in glass evacuated exsiccator，The right middle of top pressure closure is provided with exhaustor 13，The right-hand member of exhaustor is provided with exhaust connector 16，The upper end of CO2 absorber system is provided with sealing lid，The upper end sealing lid is provided with air inlet pipe 17，The exhaust connector of exhaustor is installed and is connected to seal in the air inlet pipe covering end.
Claims (1)
- null1. a culture device for anaerobic bacteria，By carbon-dioxide generator、Glass evacuated exsiccator and CO2 absorber system composition，It is characterized in that: described carbon-dioxide generator (1) is a conical flask，The upper end of conical flask is provided with sealing-plug (2)，The upper end of sealing-plug is provided with separatory funnel (5)，The upper end of separatory funnel is provided with gag (6)，The lower end of separatory funnel is provided with switch (4)，The lower end of switch is provided with leakage pipe (3)，The lower end of leakage pipe is inserted in conical flask by the left portion of sealing-plug，The right side of conical flask is provided with glass evacuated exsiccator (9)，The right side of glass evacuated exsiccator is provided with CO2 absorber system (18)，The upper end of described glass evacuated exsiccator is provided with upper cover (14)，The upper end of upper cover is provided with top pressure closure (12)，The right middle of conical flask sealing-plug is provided with escape pipe (7)，The right-hand member of escape pipe is provided with inlet pipe connection (11)，The left end of inlet pipe connection is provided with air inlet union joint (8)，The left end of inlet pipe connection is fastened on escape pipe by air inlet union joint，The right-hand member of inlet pipe connection is fixed on the top pressure closure of glass evacuated exsiccator upper end，The lower end of top pressure closure is provided with grass tube (10)，The gas outlet of grass tube is arranged on the bottom of glass evacuated exsiccator，Oxygen concentration detection indicator (15) it is provided with in glass evacuated exsiccator，The right middle of top pressure closure is provided with exhaustor (13)，The right-hand member of exhaustor is provided with exhaust connector (16)，The upper end of described CO2 absorber system is provided with sealing lid，The upper end sealing lid is provided with air inlet pipe (17)，The exhaust connector of exhaustor is installed and is connected to seal in the air inlet pipe covering end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267957.2A CN104059846B (en) | 2014-06-17 | 2014-06-17 | A kind of culture device for anaerobic bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267957.2A CN104059846B (en) | 2014-06-17 | 2014-06-17 | A kind of culture device for anaerobic bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104059846A CN104059846A (en) | 2014-09-24 |
| CN104059846B true CN104059846B (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410267957.2A Expired - Fee Related CN104059846B (en) | 2014-06-17 | 2014-06-17 | A kind of culture device for anaerobic bacteria |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104789453A (en) * | 2015-04-01 | 2015-07-22 | 河南科技大学 | Laboratory anaerobic immobilized fermentation device and anaerobic fermentation method |
| CN104707157A (en) * | 2015-04-02 | 2015-06-17 | 苏州神林堂中医药研究所 | Medical instrument loading device |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130006409A (en) * | 2012-12-21 | 2013-01-16 | 한국생명공학연구원 | Photobioreactor and microalgae culture method, and microalgae harvesting method by using that |
| CN103091194B (en) * | 2013-02-05 | 2015-03-11 | 武汉钢铁(集团)公司 | Carbon dioxide content determination apparatus |
| CN203096082U (en) * | 2013-02-21 | 2013-07-31 | 哈尔滨师范大学 | Anaerobic bioreactor |
| CN203393132U (en) * | 2013-06-27 | 2014-01-15 | 昆明理工大学 | Anaerobic cultivation device |
| CN203890351U (en) * | 2014-06-17 | 2014-10-22 | 王明跃 | Anaerobic bacterium culture device |
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| CN104059846A (en) | 2014-09-24 |
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Granted publication date: 20160817 Termination date: 20200617 |
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