CN201756550U - Fully-sealed bacteria collection ampoule incubator - Google Patents
Fully-sealed bacteria collection ampoule incubator Download PDFInfo
- Publication number
- CN201756550U CN201756550U CN 201020240360 CN201020240360U CN201756550U CN 201756550 U CN201756550 U CN 201756550U CN 201020240360 CN201020240360 CN 201020240360 CN 201020240360 U CN201020240360 U CN 201020240360U CN 201756550 U CN201756550 U CN 201756550U
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- Prior art keywords
- ampoule
- liquid
- pipe
- collection bacterium
- sample
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- 239000003708 ampul Substances 0.000 title claims abstract description 90
- 241000894006 Bacteria Species 0.000 title claims abstract description 57
- 239000007788 liquid Substances 0.000 claims abstract description 57
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- 239000002699 waste material Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 8
- 230000000813 microbial effect Effects 0.000 abstract description 7
- 238000011109 contamination Methods 0.000 abstract description 4
- 238000011010 flushing procedure Methods 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 230000009193 crawling Effects 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 6
- 230000036512 infertility Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 210000001503 joint Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a fully-sealed bacteria collection ampoule incubator, which comprises a bacteria collection ampoule system, a sample and liquid adding system and a crawling liquid drainage system, wherein the sample and liquid adding system and the bacteria collection ampoule system are connected through a liquid inlet pipe, and the bacteria collection ampoule system and the crawling liquid drainage system are connected through a liquid drainage pipe. Compared with the common ampoule, the utility model can achieve such functions in a fully-sealed sterile system as realizing sample microbial enrichment (bacteria collection function), flushing membrane to eliminate sample antibacterial activity interference (anti-interference function), adding a culture medium to cultivate recovery microbes (cultivation function), and being placed into a calorimeter channel to record the growth and metabolism conditions of microbes (recording function); and the whole operation process from sampling to cultivation eliminates the possibility of sample or culture medium contamination caused by external factors, that is, the possibility of misjudged false positive of microbial contamination on samples is eliminated, so that the testing accuracy is remarkably improved.
Description
Technical field
The utility model belongs to the microorganism checking field, relates to a kind of totally-enclosed collection bacterium ampoule incubator specifically.
Background technology
Existing common ampoule is simple in structure, can not satisfy product microbial contamination and check that (sterility test) desired isolated external environment (avoiding secondary pollution), enriched microorganism eliminate the requirement of product bacteriostasis property simultaneously.Therefore, need a kind of totally-enclosed collection bacterium ampoule to realize above-mentioned functions in the practice.Principle of design of the present utility model comprises: (1) sterility: adopt suitable sterilising method to guarantee the sterility of bacteria gathering device self; (2) stopping property: guarantee effective isolation of internal system and external environment; (3) collection bacterium property: the collection bacterium device that preparation is necessary, realize sample microbial enrichment and biocidal property are eliminated, and can be according to the suitable filter membrane of properties of samples configuration to be checked; (4) thermo-sensitivity: system material can make microorganism growth metabolism heat be detected delicately by calorimeter; (5) resistance to pressure: system can satisfy the requirement of collection bacterium process negative pressure, and avoids microbial damage; (6) tolerance: can satisfy sterility test sample size needs, enough checking abilities are arranged; (7) simplicity: easy and simple to handle, the automatization performance is good, has automatic prompting function as a result; (8) economy: economy is easy to get, and can produce in batches and be applicable.
Summary of the invention
The purpose of this utility model is in order to solve the above-mentioned defective of common ampoule, to provide a kind of totally enclosed collection bacterium to filter the ampoule incubator.
Realize that the above-mentioned purpose the technical solution of the utility model is, a kind of totally-enclosed collection bacterium ampoule incubator, add liquid system and the wriggling drainage system is formed by collection bacterium ampoule system, application of sample, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe, and collection bacterium ampoule system and wriggling drainage system link together by liquid discharge pipe.
Collection bacterium ampoule system comprises the ampoule body, and sealing and fixing rubber seal plug on the bottleneck of ampoule body, liquid-inlet pipe, liquid discharge pipe and vapor pipe stretch in the ampoule body after penetrating rubber seal plug.The built-in filter of ampoule body, the bottom of filter is covered with filter membrane, the feed liquor mouth of pipe in the top of filter and the ampoule is connected, the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended the ampoule bottom, be separately installed with liquid inlet control valve, discharge opeing control valve and gas exhausting valve on liquid-inlet pipe, liquid discharge pipe and the vapor pipe of ampoule body outside, the vapor pipe top connects air filter.
Application of sample adds the feeding device that liquid system comprises sample/medium container and band air filter.
The wriggling drainage system comprises a peristaltic pump, and the wriggling pump outlet connects the waste collection device.
On liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system, an input duct junctor is installed, when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
Input duct junctor and Drainage pipe junctor are plug mouthful formula, and the plug mouth of input duct junctor and Drainage pipe junctor can dock and form the sealing-duct junctor.
Ampoule indicates scale marks as glass structure or transparent rigid plastics structure on one's body at ampoule.
Liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
Liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
Vapor pipe is by the top band air filter of silica gel hose connection, the stainless steel syringe needle of tail end hollow side wall opening.
The part that liquid-inlet pipe stretches into the ampoule body can be up-thin-low-thick tapered tube, and filter is fixed on the lower end of tapered tube.The upper end outside surface of described tapered tube is a helicitic texture, and the lower surface of rubber seal plug is fixed an internal thread interface, and tapered tube can be connected with this internal thread interface by helicitic texture.
The utility model is compared with common ampoule, can realize the sample microbial enrichment in the full closed aseptic system, that is, and and collection bacterium function; The flushing film is eliminated the sample bacteriostatic activity and is disturbed, that is, and and the anti-interference function; Add substratum and cultivate the recovery microorganism, that is, cultivate function; And insert the hot metabolic condition of calorimeter passage record microorganism growth, that is, and writing function; Cultivate the possibility that the entire operation process has all been got rid of extraneous factor contaminated samples or substratum from being sampled to, promptly eliminate the possibility (false positive) of sample microbial contamination false positive erroneous judgement, significantly improved the accuracy that detects.
Description of drawings
Fig. 1 is the structural representation of the totally-enclosed collection of the utility model bacterium ampoule incubator;
Fig. 2 is that input duct junctor and the butt joint of Drainage pipe junctor plug mouth form the sealing-duct junctor, and extracts the totally-enclosed collection bacterium ampoule incubator structural representation behind the vapor pipe;
Fig. 3 is the structural representation of liquid-inlet pipe when stretching into part in the ampoule body and being deformed into tapered tube;
Fig. 4 is the structural representation of tapered tube;
Among the figure, 1, the ampoule body; 2, filter;
3, rubber seal plug; 4, liquid-inlet pipe;
5, liquid discharge pipe; 6, vapor pipe;
7, liquid inlet control valve; 8, discharge opeing control valve;
9, scale marks; 10, feeding device;
11, input duct junctor; 12, sample/medium container;
13, peristaltic pump; 14, waste collection device;
15, filter membrane; 16, air filter;
17, gas exhausting valve; 18, Drainage pipe junctor;
19, sealing-duct junctor; 20, tapered tube;
21, the helicitic texture of tapered tube upper end;
22, the internal thread interface of rubber seal plug.
Embodiment
Below in conjunction with accompanying drawing the utility model is specifically described.
As shown in Figure 1, totally-enclosed collection bacterium ampoule incubator adds liquid system by collection bacterium ampoule system, application of sample and the drainage system of wriggling is formed, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe 4, and collection bacterium ampoule system and wriggling drainage system link together by liquid discharge pipe 5.
Preferred structure is that collection bacterium ampoule system comprises ampoule body 1, sealing and fixing rubber seal plug 3 on the bottleneck of ampoule body.Liquid-inlet pipe 4, liquid discharge pipe 5 and vapor pipe 6 stretch in the ampoule body after penetrating rubber seal plug.The built-in filter 2 of ampoule body, the bottom of filter is covered with filter membrane 15, and the feed liquor mouth of pipe in the top of filter and the ampoule is connected, and the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended bottom the ampoule.Filter membrane can set in advance according to the difference of filtering object and be different materials.Be separately installed with liquid inlet control valve 7, discharge opeing control valve 8 and gas exhausting valve 17 on the liquid-inlet pipe of ampoule body outside and liquid discharge pipe, the vapor pipe top connects air filter 16.
Preferred structure is that application of sample adds the feeding device 10 that liquid system comprises sample/medium container 12 and band air filter.
Preferred structure is that the wriggling drainage system comprises a peristaltic pump 13, wriggling pump outlet connection waste collection device 14.
Preferred structure is, on liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system input duct junctor 11 is installed, and when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor 18 is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
Input duct junctor and Drainage pipe junctor are plug mouthful formula, and the plug mouth of input duct junctor and Drainage pipe junctor can dock and form sealing-duct junctor 19.
After in collection bacterium ampoule incubator, finishing the injecting program of filtration and substratum, from input duct junctor 11 plug mouths application of sample is added liquid system and separate with collection bacterium ampoule system; From Drainage pipe junctor 18 plug mouth will wriggle drainage system with collect bacterium ampoule system and separate; Then input duct junctor plug mouth and the butt joint of Drainage pipe junctor plug mouth are formed sealing-duct junctor 19, make collection bacterium culture vessel be in sealed state, as shown in Figure 2.
Preferred structure is to indicate scale marks 9 on one's body at ampoule.Can determine to indicate precision as required, as indicating scale markss such as 5mL, 10mL, 15mL.
Preferred structure is, ampoule is as glass structure or rigid plastics structure, and transparent material can guarantee the accuracy of visual observation.
Preferred structure is that liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
Preferred structure is that liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
Preferred structure is that vapor pipe is by the top band air filter of silica gel hose connection, the stainless steel syringe needle of tail end hollow side wall opening.
In addition, the utility model also provides a kind of distressed structure of liquid-inlet pipe.
As shown in Figure 3 and Figure 4, the part that liquid-inlet pipe stretches into the ampoule body can be up-thin-low-thick tapered tube 20, and filter is fixed on the lower end of tapered tube.The upper end outside surface of described tapered tube is a helicitic texture 21, and the lower surface of rubber seal plug is fixed an internal thread interface 22, and tapered tube can be connected with this internal thread interface by helicitic texture 21.
Using method of the present utility model is:
1, application of sample being added liquid system, collection bacterium ampoule system, wriggling drainage system links together in turn, just feeding device 10 is connected on sample/medium container 12, to connecting, is sample to be checked with the plug mouth of input duct junctor 11 in sample/medium container 12; Again with the plug mouth of Drainage pipe junctor 18 also to connecting.
2, close gas exhausting valve 17, open liquid inlet control valve 7, discharge opeing control valve 8, peristaltic pump 13, adjust flow velocity, slow filtered sample is also got rid of soup; After above-mentioned steps is finished, open exhaust-duct control valve 17, close peristaltic pump 13, liquid inlet control valve 7, discharge opeing control valve 8;
3, replacing sample/medium container is aseptic scavenging solution, closes exhaust-duct control valve 17, opens liquid inlet control valve 7, discharge opeing control valve 8, peristaltic pump 13, adjusts flow velocity, and cleaning filter membranes is also got rid of waste liquid;
4, close peristaltic pump 13, discharge opeing control valve 8, liquid inlet control valve 7, open gas exhausting valve 17, replacing sample/medium container 12 interior samples is corresponding substratum;
5, close gas exhausting valve 17, open discharge opeing control valve 8, peristaltic pump 13, make to be negative pressure state in the bottle; And take out brokenly filter membrane 15, make the interior UNICOM of filter 2 and bottle;
6, close discharge opeing control valve 8, peristaltic pump 13, open liquid inlet control valve 7, make substratum add to corresponding scale, close liquid inlet control valve 7;
If need a certain proportion of air to impel microorganism to grow fast in 7 culturing process, just open gas exhausting valve 17 and in collection bacterium ampoule incubator, inject air in proportion, extract exhaust-duct 6 then; If culturing process does not need air, just directly extract exhaust-duct 6;
8, open the plug mouth of input duct junctor 11 and Drainage pipe junctor 18, and the plug mouth combination of these two pipe jointers is docking together, form sealing-duct junctor 19, make collection bacterium ampoule incubator be in sealed state;
9, the collection bacterium ampoule incubator with sealed state places relevant detection instrument/environment, obtains test result of samples.
Technique scheme has only embodied the optimal technical scheme of technical solutions of the utility model; those skilled in the art to some part wherein some changes that may make all embodied principle of the present utility model, belong within the protection domain of the present utility model.
Claims (12)
1. totally-enclosed collection bacterium ampoule incubator, it is characterized in that, described ampoule incubator adds liquid system by collection bacterium ampoule system, application of sample and the drainage system of wriggling is formed, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe (4), and the collection bacterium ampoule system and the drainage system of wriggling link together by liquid discharge pipe (5).
2. totally-enclosed collection bacterium ampoule incubator according to claim 1, it is characterized in that, wherein collect bacterium ampoule system and comprise ampoule body (1), sealing and fixing rubber seal plug (3) on the bottleneck of ampoule body, liquid-inlet pipe (4), liquid discharge pipe (5) and vapor pipe (6) stretch in the ampoule body after penetrating rubber seal plug, the built-in filter of ampoule body (2), the bottom of filter is covered with filter membrane (15), the feed liquor mouth of pipe in the top of filter and the ampoule is connected, the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended the ampoule bottom, liquid-inlet pipe in ampoule body outside, be separately installed with liquid inlet control valve (7) on liquid discharge pipe and the vapor pipe, discharge opeing control valve (8) and gas exhausting valve (17), the vapor pipe top connects air filter (16).
3. totally-enclosed collection bacterium ampoule incubator according to claim 1 is characterized in that, application of sample adds the feeding device (10) that liquid system comprises sample/medium container (12) and band air filter.
4. totally-enclosed collection bacterium ampoule incubator according to claim 1 is characterized in that, the wriggling drainage system comprises a peristaltic pump (13), and the wriggling pump outlet connects waste collection device (14).
5. totally-enclosed collection bacterium ampoule incubator according to claim 2, it is characterized in that, on liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system, an input duct junctor (11) is installed, when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor (18) is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
6. totally-enclosed collection bacterium ampoule incubator according to claim 5, it is characterized in that, input duct junctor (11) and Drainage pipe junctor (18) are plug mouthful formula, and the plug mouth of input duct connector tube and liquid discharge pipe pipe jointer can dock and form sealing-duct junctor (19).
7. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that ampoule indicates scale marks (9) as glass structure or transparent rigid plastics structure on one's body at ampoule.
8. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
9. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
10. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that, vapor pipe is the stainless steel syringe needle by the top band air filter of silica gel hose connection.
11. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that the part that liquid-inlet pipe stretches into the ampoule body is up-thin-low-thick tapered tube (20), filter (2) is fixed on the lower end of tapered tube.
12. totally-enclosed collection bacterium ampoule incubator according to claim 11, it is characterized in that, the upper end outside surface of described tapered tube is helicitic texture (21), an internal thread interface (22) is fixed at the lower surface feed liquor mouth of pipe place of rubber seal plug, and tapered tube can be connected with this internal thread interface by helicitic texture.
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CN 201020240360 CN201756550U (en) | 2010-06-29 | 2010-06-29 | Fully-sealed bacteria collection ampoule incubator |
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Cited By (8)
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CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
WO2015101326A1 (en) * | 2013-12-31 | 2015-07-09 | 牛刚 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
CN106834421A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled powder test sample in XiLin |
CN106834422A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule |
CN107022477A (en) * | 2017-03-21 | 2017-08-08 | 朱彩玲 | Sealed effectual microorganism limit detector |
CN107384757A (en) * | 2017-08-02 | 2017-11-24 | 山东省食品药品检验研究院 | Aspergillus niger spore culture, the device and method for separating and preparing suspension |
CN109337792A (en) * | 2018-09-29 | 2019-02-15 | 山西晋城无烟煤矿业集团有限责任公司 | Collection, the save set of a kind of portable strain and its inhereditary material |
CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
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2010
- 2010-06-29 CN CN 201020240360 patent/CN201756550U/en not_active Expired - Lifetime
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CN101893589B (en) * | 2010-06-29 | 2012-10-17 | 中国人民解放军第三0二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
KR101807984B1 (en) * | 2013-12-31 | 2017-12-11 | 왕, 화산 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
WO2015101326A1 (en) * | 2013-12-31 | 2015-07-09 | 牛刚 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
AU2014375463B2 (en) * | 2013-12-31 | 2017-05-25 | Gang Niu | Full-automatic microbial detection and enrichment system and enrichment method thereof |
CN106834421A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled powder test sample in XiLin |
CN106834422A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule |
CN106834421B (en) * | 2017-01-16 | 2019-08-16 | 杭州电子科技大学 | Full-automatic assembly line collection bacterium operating method and system towards the bottled powder test sample in XiLin |
CN107022477A (en) * | 2017-03-21 | 2017-08-08 | 朱彩玲 | Sealed effectual microorganism limit detector |
CN107022477B (en) * | 2017-03-21 | 2019-09-10 | 山西华普卫生检测技术有限公司 | Sealed effectual microorganism limit detector |
CN107384757A (en) * | 2017-08-02 | 2017-11-24 | 山东省食品药品检验研究院 | Aspergillus niger spore culture, the device and method for separating and preparing suspension |
CN109337792A (en) * | 2018-09-29 | 2019-02-15 | 山西晋城无烟煤矿业集团有限责任公司 | Collection, the save set of a kind of portable strain and its inhereditary material |
CN109337792B (en) * | 2018-09-29 | 2022-03-22 | 山西晋城无烟煤矿业集团有限责任公司 | Portable strain and genetic material collecting and storing device thereof |
CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
CN116042383B (en) * | 2023-01-06 | 2023-10-10 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
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Granted publication date: 20110309 Effective date of abandoning: 20130306 |
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