CN201756550U - Fully-sealed bacteria collection ampoule incubator - Google Patents
Fully-sealed bacteria collection ampoule incubator Download PDFInfo
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- CN201756550U CN201756550U CN 201020240360 CN201020240360U CN201756550U CN 201756550 U CN201756550 U CN 201756550U CN 201020240360 CN201020240360 CN 201020240360 CN 201020240360 U CN201020240360 U CN 201020240360U CN 201756550 U CN201756550 U CN 201756550U
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Abstract
本实用新型公开了一种全封闭集菌安瓿培养器，由集菌安瓿系统、加样加液系统和蠕动排液系统组成。所述加样加液系统与集菌安瓿系统之间通过进液管连接在一起，集菌安瓿系统和蠕动排液系统通过排液管连接在一起。本实用新型和普通安瓿相比，可以在全封闭无菌体系中实现样品微生物富集(集菌功能)、冲洗薄膜消除样品抑菌活性干扰(抗扰功能)、加入培养基培育复苏微生物(培养功能)以及置入微量量热仪通道记录微生物生长热代谢状况(记录功能)，从取样到培养整个操作过程都排除了外界因素污染样品或培养基的可能性，即消除样品微生物污染假阳性误判的可能性，显著提高了检测的准确性。
The utility model discloses a fully enclosed bacteria-collecting ampoule incubator, which is composed of a bacteria-collecting ampoule system, a sample adding liquid system and a peristaltic liquid draining system. The sample adding and liquid system is connected with the bacteria collection ampoule system through a liquid inlet pipe, and the bacteria collection ampoule system and the peristaltic liquid drainage system are connected together through a liquid discharge pipe. Compared with ordinary ampoules, the utility model can realize the enrichment of sample microorganisms (bacteria collection function) in a fully enclosed aseptic system, wash the film to eliminate the interference of sample antibacterial activity (anti-disturbance function), and add medium to cultivate and recover microorganisms (cultivation function). Function) and microcalorimeter channel to record the thermal metabolism of microbial growth (recording function), the possibility of contamination of the sample or medium by external factors is eliminated during the entire operation process from sampling to cultivation, that is, to eliminate false positives and errors of microbial contamination of samples The possibility of judgment can significantly improve the accuracy of detection.
Description
Technical field
The utility model belongs to the microorganism checking field, relates to a kind of totally-enclosed collection bacterium ampoule incubator specifically.
Background technology
Existing common ampoule is simple in structure, can not satisfy product microbial contamination and check that (sterility test) desired isolated external environment (avoiding secondary pollution), enriched microorganism eliminate the requirement of product bacteriostasis property simultaneously.Therefore, need a kind of totally-enclosed collection bacterium ampoule to realize above-mentioned functions in the practice.Principle of design of the present utility model comprises: (1) sterility: adopt suitable sterilising method to guarantee the sterility of bacteria gathering device self; (2) stopping property: guarantee effective isolation of internal system and external environment; (3) collection bacterium property: the collection bacterium device that preparation is necessary, realize sample microbial enrichment and biocidal property are eliminated, and can be according to the suitable filter membrane of properties of samples configuration to be checked; (4) thermo-sensitivity: system material can make microorganism growth metabolism heat be detected delicately by calorimeter; (5) resistance to pressure: system can satisfy the requirement of collection bacterium process negative pressure, and avoids microbial damage; (6) tolerance: can satisfy sterility test sample size needs, enough checking abilities are arranged; (7) simplicity: easy and simple to handle, the automatization performance is good, has automatic prompting function as a result; (8) economy: economy is easy to get, and can produce in batches and be applicable.
Summary of the invention
The purpose of this utility model is in order to solve the above-mentioned defective of common ampoule, to provide a kind of totally enclosed collection bacterium to filter the ampoule incubator.
Realize that the above-mentioned purpose the technical solution of the utility model is, a kind of totally-enclosed collection bacterium ampoule incubator, add liquid system and the wriggling drainage system is formed by collection bacterium ampoule system, application of sample, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe, and collection bacterium ampoule system and wriggling drainage system link together by liquid discharge pipe.
Collection bacterium ampoule system comprises the ampoule body, and sealing and fixing rubber seal plug on the bottleneck of ampoule body, liquid-inlet pipe, liquid discharge pipe and vapor pipe stretch in the ampoule body after penetrating rubber seal plug.The built-in filter of ampoule body, the bottom of filter is covered with filter membrane, the feed liquor mouth of pipe in the top of filter and the ampoule is connected, the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended the ampoule bottom, be separately installed with liquid inlet control valve, discharge opeing control valve and gas exhausting valve on liquid-inlet pipe, liquid discharge pipe and the vapor pipe of ampoule body outside, the vapor pipe top connects air filter.
Application of sample adds the feeding device that liquid system comprises sample/medium container and band air filter.
The wriggling drainage system comprises a peristaltic pump, and the wriggling pump outlet connects the waste collection device.
On liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system, an input duct junctor is installed, when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
Input duct junctor and Drainage pipe junctor are plug mouthful formula, and the plug mouth of input duct junctor and Drainage pipe junctor can dock and form the sealing-duct junctor.
Ampoule indicates scale marks as glass structure or transparent rigid plastics structure on one's body at ampoule.
Liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
Liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
Vapor pipe is by the top band air filter of silica gel hose connection, the stainless steel syringe needle of tail end hollow side wall opening.
The part that liquid-inlet pipe stretches into the ampoule body can be up-thin-low-thick tapered tube, and filter is fixed on the lower end of tapered tube.The upper end outside surface of described tapered tube is a helicitic texture, and the lower surface of rubber seal plug is fixed an internal thread interface, and tapered tube can be connected with this internal thread interface by helicitic texture.
The utility model is compared with common ampoule, can realize the sample microbial enrichment in the full closed aseptic system, that is, and and collection bacterium function; The flushing film is eliminated the sample bacteriostatic activity and is disturbed, that is, and and the anti-interference function; Add substratum and cultivate the recovery microorganism, that is, cultivate function; And insert the hot metabolic condition of calorimeter passage record microorganism growth, that is, and writing function; Cultivate the possibility that the entire operation process has all been got rid of extraneous factor contaminated samples or substratum from being sampled to, promptly eliminate the possibility (false positive) of sample microbial contamination false positive erroneous judgement, significantly improved the accuracy that detects.
Description of drawings
Fig. 1 is the structural representation of the totally-enclosed collection of the utility model bacterium ampoule incubator;
Fig. 2 is that input duct junctor and the butt joint of Drainage pipe junctor plug mouth form the sealing-duct junctor, and extracts the totally-enclosed collection bacterium ampoule incubator structural representation behind the vapor pipe;
Fig. 3 is the structural representation of liquid-inlet pipe when stretching into part in the ampoule body and being deformed into tapered tube;
Fig. 4 is the structural representation of tapered tube;
Among the figure, 1, the ampoule body; 2, filter;
3, rubber seal plug; 4, liquid-inlet pipe;
5, liquid discharge pipe; 6, vapor pipe;
7, liquid inlet control valve; 8, discharge opeing control valve;
9, scale marks; 10, feeding device;
11, input duct junctor; 12, sample/medium container;
13, peristaltic pump; 14, waste collection device;
15, filter membrane; 16, air filter;
17, gas exhausting valve; 18, Drainage pipe junctor;
19, sealing-duct junctor; 20, tapered tube;
21, the helicitic texture of tapered tube upper end;
22, the internal thread interface of rubber seal plug.
Embodiment
Below in conjunction with accompanying drawing the utility model is specifically described.
As shown in Figure 1, totally-enclosed collection bacterium ampoule incubator adds liquid system by collection bacterium ampoule system, application of sample and the drainage system of wriggling is formed, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe 4, and collection bacterium ampoule system and wriggling drainage system link together by liquid discharge pipe 5.
Preferred structure is that collection bacterium ampoule system comprises ampoule body 1, sealing and fixing rubber seal plug 3 on the bottleneck of ampoule body.Liquid-inlet pipe 4, liquid discharge pipe 5 and vapor pipe 6 stretch in the ampoule body after penetrating rubber seal plug.The built-in filter 2 of ampoule body, the bottom of filter is covered with filter membrane 15, and the feed liquor mouth of pipe in the top of filter and the ampoule is connected, and the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended bottom the ampoule.Filter membrane can set in advance according to the difference of filtering object and be different materials.Be separately installed with liquid inlet control valve 7, discharge opeing control valve 8 and gas exhausting valve 17 on the liquid-inlet pipe of ampoule body outside and liquid discharge pipe, the vapor pipe top connects air filter 16.
Preferred structure is that application of sample adds the feeding device 10 that liquid system comprises sample/medium container 12 and band air filter.
Preferred structure is that the wriggling drainage system comprises a peristaltic pump 13, wriggling pump outlet connection waste collection device 14.
Preferred structure is, on liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system input duct junctor 11 is installed, and when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor 18 is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
Input duct junctor and Drainage pipe junctor are plug mouthful formula, and the plug mouth of input duct junctor and Drainage pipe junctor can dock and form sealing-duct junctor 19.
After in collection bacterium ampoule incubator, finishing the injecting program of filtration and substratum, from input duct junctor 11 plug mouths application of sample is added liquid system and separate with collection bacterium ampoule system; From Drainage pipe junctor 18 plug mouth will wriggle drainage system with collect bacterium ampoule system and separate; Then input duct junctor plug mouth and the butt joint of Drainage pipe junctor plug mouth are formed sealing-duct junctor 19, make collection bacterium culture vessel be in sealed state, as shown in Figure 2.
Preferred structure is to indicate scale marks 9 on one's body at ampoule.Can determine to indicate precision as required, as indicating scale markss such as 5mL, 10mL, 15mL.
Preferred structure is, ampoule is as glass structure or rigid plastics structure, and transparent material can guarantee the accuracy of visual observation.
Preferred structure is that liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
Preferred structure is that liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
Preferred structure is that vapor pipe is by the top band air filter of silica gel hose connection, the stainless steel syringe needle of tail end hollow side wall opening.
In addition, the utility model also provides a kind of distressed structure of liquid-inlet pipe.
As shown in Figure 3 and Figure 4, the part that liquid-inlet pipe stretches into the ampoule body can be up-thin-low-thick tapered tube 20, and filter is fixed on the lower end of tapered tube.The upper end outside surface of described tapered tube is a helicitic texture 21, and the lower surface of rubber seal plug is fixed an internal thread interface 22, and tapered tube can be connected with this internal thread interface by helicitic texture 21.
Using method of the present utility model is:
1, application of sample being added liquid system, collection bacterium ampoule system, wriggling drainage system links together in turn, just feeding device 10 is connected on sample/medium container 12, to connecting, is sample to be checked with the plug mouth of input duct junctor 11 in sample/medium container 12; Again with the plug mouth of Drainage pipe junctor 18 also to connecting.
2, close gas exhausting valve 17, open liquid inlet control valve 7, discharge opeing control valve 8, peristaltic pump 13, adjust flow velocity, slow filtered sample is also got rid of soup; After above-mentioned steps is finished, open exhaust-duct control valve 17, close peristaltic pump 13, liquid inlet control valve 7, discharge opeing control valve 8;
3, replacing sample/medium container is aseptic scavenging solution, closes exhaust-duct control valve 17, opens liquid inlet control valve 7, discharge opeing control valve 8, peristaltic pump 13, adjusts flow velocity, and cleaning filter membranes is also got rid of waste liquid;
4, close peristaltic pump 13, discharge opeing control valve 8, liquid inlet control valve 7, open gas exhausting valve 17, replacing sample/medium container 12 interior samples is corresponding substratum;
5, close gas exhausting valve 17, open discharge opeing control valve 8, peristaltic pump 13, make to be negative pressure state in the bottle; And take out brokenly filter membrane 15, make the interior UNICOM of filter 2 and bottle;
6, close discharge opeing control valve 8, peristaltic pump 13, open liquid inlet control valve 7, make substratum add to corresponding scale, close liquid inlet control valve 7;
If need a certain proportion of air to impel microorganism to grow fast in 7 culturing process, just open gas exhausting valve 17 and in collection bacterium ampoule incubator, inject air in proportion, extract exhaust-duct 6 then; If culturing process does not need air, just directly extract exhaust-duct 6;
8, open the plug mouth of input duct junctor 11 and Drainage pipe junctor 18, and the plug mouth combination of these two pipe jointers is docking together, form sealing-duct junctor 19, make collection bacterium ampoule incubator be in sealed state;
9, the collection bacterium ampoule incubator with sealed state places relevant detection instrument/environment, obtains test result of samples.
Technique scheme has only embodied the optimal technical scheme of technical solutions of the utility model; those skilled in the art to some part wherein some changes that may make all embodied principle of the present utility model, belong within the protection domain of the present utility model.
Claims (12)
1. totally-enclosed collection bacterium ampoule incubator, it is characterized in that, described ampoule incubator adds liquid system by collection bacterium ampoule system, application of sample and the drainage system of wriggling is formed, described application of sample adds between liquid system and the collection bacterium ampoule system and links together by liquid-inlet pipe (4), and the collection bacterium ampoule system and the drainage system of wriggling link together by liquid discharge pipe (5).
2. totally-enclosed collection bacterium ampoule incubator according to claim 1, it is characterized in that, wherein collect bacterium ampoule system and comprise ampoule body (1), sealing and fixing rubber seal plug (3) on the bottleneck of ampoule body, liquid-inlet pipe (4), liquid discharge pipe (5) and vapor pipe (6) stretch in the ampoule body after penetrating rubber seal plug, the built-in filter of ampoule body (2), the bottom of filter is covered with filter membrane (15), the feed liquor mouth of pipe in the top of filter and the ampoule is connected, the discharge opeing mouth of pipe in the ampoule is crossed filter and is extended the ampoule bottom, liquid-inlet pipe in ampoule body outside, be separately installed with liquid inlet control valve (7) on liquid discharge pipe and the vapor pipe, discharge opeing control valve (8) and gas exhausting valve (17), the vapor pipe top connects air filter (16).
3. totally-enclosed collection bacterium ampoule incubator according to claim 1 is characterized in that, application of sample adds the feeding device (10) that liquid system comprises sample/medium container (12) and band air filter.
4. totally-enclosed collection bacterium ampoule incubator according to claim 1 is characterized in that, the wriggling drainage system comprises a peristaltic pump (13), and the wriggling pump outlet connects waste collection device (14).
5. totally-enclosed collection bacterium ampoule incubator according to claim 2, it is characterized in that, on liquid inlet control valve and application of sample add liquid-inlet pipe between the liquid system, an input duct junctor (11) is installed, when disconnecting this pipe jointer, application of sample can be added liquid system and separate with collection bacterium ampoule system; On the liquid discharge pipe between discharge opeing control valve and the wriggling drainage system, a Drainage pipe junctor (18) is installed, when disconnecting this pipe jointer, the wriggling drainage system can be separated with collection bacterium ampoule system.
6. totally-enclosed collection bacterium ampoule incubator according to claim 5, it is characterized in that, input duct junctor (11) and Drainage pipe junctor (18) are plug mouthful formula, and the plug mouth of input duct connector tube and liquid discharge pipe pipe jointer can dock and form sealing-duct junctor (19).
7. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that ampoule indicates scale marks (9) as glass structure or transparent rigid plastics structure on one's body at ampoule.
8. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that liquid inlet control valve, discharge opeing control valve and gas exhausting valve are the bayonet socket valve.
9. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that liquid-inlet pipe, liquid discharge pipe and vapor pipe are silica gel hose.
10. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that, vapor pipe is the stainless steel syringe needle by the top band air filter of silica gel hose connection.
11. totally-enclosed collection bacterium ampoule incubator according to claim 2 is characterized in that the part that liquid-inlet pipe stretches into the ampoule body is up-thin-low-thick tapered tube (20), filter (2) is fixed on the lower end of tapered tube.
12. totally-enclosed collection bacterium ampoule incubator according to claim 11, it is characterized in that, the upper end outside surface of described tapered tube is helicitic texture (21), an internal thread interface (22) is fixed at the lower surface feed liquor mouth of pipe place of rubber seal plug, and tapered tube can be connected with this internal thread interface by helicitic texture.
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| CN 201020240360 CN201756550U (en) | 2010-06-29 | 2010-06-29 | Fully-sealed bacteria collection ampoule incubator |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | A kind of sterility inspection method and fully enclosed bacteria-collecting ampoule incubator used therefor |
| WO2015101326A1 (en) * | 2013-12-31 | 2015-07-09 | 牛刚 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| CN106834422A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule |
| CN106834421A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled powder test sample in XiLin |
| CN107022477A (en) * | 2017-03-21 | 2017-08-08 | 朱彩玲 | Sealed effectual microorganism limit detector |
| CN107384757A (en) * | 2017-08-02 | 2017-11-24 | 山东省食品药品检验研究院 | Aspergillus niger spore culture, the device and method for separating and preparing suspension |
| CN109337792A (en) * | 2018-09-29 | 2019-02-15 | 山西晋城无烟煤矿业集团有限责任公司 | Collection, the save set of a kind of portable strain and its inhereditary material |
| CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | A kind of culture system and culture method of marine microorganisms used for wine making |
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- 2010-06-29 CN CN 201020240360 patent/CN201756550U/en not_active Expired - Lifetime
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893589B (en) * | 2010-06-29 | 2012-10-17 | 中国人民解放军第三0二医院 | A kind of sterility inspection method and fully enclosed bacteria-collecting ampoule incubator used therein |
| CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | A kind of sterility inspection method and fully enclosed bacteria-collecting ampoule incubator used therefor |
| KR101807984B1 (en) * | 2013-12-31 | 2017-12-11 | 왕, 화산 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| WO2015101326A1 (en) * | 2013-12-31 | 2015-07-09 | 牛刚 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| AU2014375463B2 (en) * | 2013-12-31 | 2017-05-25 | Gang Niu | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| CN106834422A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled aqueous solution test sample of ampoule |
| CN106834421A (en) * | 2017-01-16 | 2017-06-13 | 杭州电子科技大学 | Towards the full-automatic streamline collection bacterium operating method and system of the bottled powder test sample in XiLin |
| CN106834421B (en) * | 2017-01-16 | 2019-08-16 | 杭州电子科技大学 | Fully automated assembly line bacteria collection operation method and system for vial-packed powder test products |
| CN107022477A (en) * | 2017-03-21 | 2017-08-08 | 朱彩玲 | Sealed effectual microorganism limit detector |
| CN107022477B (en) * | 2017-03-21 | 2019-09-10 | 山西华普卫生检测技术有限公司 | Sealed effectual microorganism limit detector |
| CN107384757A (en) * | 2017-08-02 | 2017-11-24 | 山东省食品药品检验研究院 | Aspergillus niger spore culture, the device and method for separating and preparing suspension |
| CN109337792A (en) * | 2018-09-29 | 2019-02-15 | 山西晋城无烟煤矿业集团有限责任公司 | Collection, the save set of a kind of portable strain and its inhereditary material |
| CN109337792B (en) * | 2018-09-29 | 2022-03-22 | 山西晋城无烟煤矿业集团有限责任公司 | Portable strain and genetic material collecting and storing device thereof |
| CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | A kind of culture system and culture method of marine microorganisms used for wine making |
| CN116042383B (en) * | 2023-01-06 | 2023-10-10 | 江苏海魄酒业股份有限公司 | A culture system and culture method of marine microorganisms used for wine making |
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Granted publication date: 20110309 Effective date of abandoning: 20130306 |
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