CN209443001U - A kind of microorganism count device - Google Patents

Abstract

The utility model relates to the technical field of microorganism detection, in particular to a microorganism counting device, comprising a water sample filtering part: a sampling syringe assembled in sequence, a one-way valve assembly, a culture chamber, a three-way valve assembly, a sampling joint assembly and a water intake bag; It also includes the part of adding medium: medium adding tube and auxiliary syringe. In the microgravity unclean state, the sample passes through the closed sterile channel of the water bag and sampling joint assembly, and is connected by the three-way valve assembly through the culture compartment. Membrane filtration, and then through the three-way valve assembly to transfer the medium addition tube and the auxiliary syringe to inject the liquid medium for microbial cultivation and counting, this device can realize the collection and transfer of samples, the filtration and cultivation of bacteria and fungi in a microgravity non-clean environment And counting, the whole process is carried out in a closed state, which not only avoids pollution during operation, but also effectively prevents internal microorganisms from polluting the external environment, making the detection results more accurate.

Description

A microbial counting device

technical field

The utility model belongs to the technical field of microorganism detection, in particular to a microorganism counting device.

Background technique

Membrane filtration method is one of the microbial detection methods of water samples. The traditional operation method is to pump the water sample into the filter container through the built-in pump of the product, filter the water sample through the filter membrane by gravity, and then paste the filter membrane with microorganisms. Microorganisms are cultivated and counted on the surface of the agar plate, but the conventional operation requires the filter membrane to be pasted on the surface of the agar plate, and the film sticking operation needs to open the filter container to transfer the filter membrane, and the open operation can easily lead to contamination in the detection process.

In the microgravity non-clean environment, the microgravity makes the filtration process complicated, and the open operation will lead to more serious pollution in the detection process, which will lead to inaccurate detection results. In order to solve this technical problem, the simple operation integrated Or the fully enclosed microbial detection device can be applied to water sample microbial detection to improve the accuracy of detection results.

For example, Chinese patent document CN206692661U discloses a pretreatment device for microbial detection, which includes a microbial culture bin and a filtrate bin that is threaded on the upper end of the filtrate bin. The push-pull chamber and the filter chamber have the same straight central axis and are connected to each other. A sealing cover is arranged on the top of the push-pull chamber, and a filter enrichment block is placed inside the filter chamber. Although the above-mentioned device has been optimized in the functional modules of filtration and enrichment, there are still risks of exposure during the introduction of samples and culture media, and it is difficult to directly view the counting results through the device.

Utility model content

Therefore, the technical problem to be solved by the utility model is to overcome the technical problem that the non-closed operation of the existing microgravity counting technology pollutes the environment in a microgravity non-clean state, and is also easily polluted by the environment, and provides a microgravity non-clean environment. It is a microbial counting device that can avoid contamination in the state, make the detection result accurate, and operate conveniently.

The utility model provides a microorganism counting device, which can be divided into a water sample filtering part and a culture medium adding part according to the operation process.

The water sample filtering part includes a sampling syringe fixedly connected and assembled in sequence during use, a one-way valve assembly arranged at the front end of the sampling syringe, a culture chamber, a three-way valve assembly, a sampling joint assembly and a water intake bag. The sample inlet of the chamber and the sample outlet of the culture chamber, the three-way valve assembly includes a first interface, a second interface, a third interface and a limit valve, the first interface is connected with the inlet of the culture chamber, and the An air filter is provided on the second interface;

The addition medium section also includes medium addition tubes and auxiliary syringes used for medium addition operations.

Preferably, the sampling syringe includes a Luer double female connector arranged at the sampling inlet; it also includes a limit column arranged inside the end of the sampling syringe and a scale arranged on the outer surface of the sampling syringe.

Preferably, the one-way valve assembly includes a one-way valve and a Luer double male connector, one end of the one-way valve is connected to the Luer double female connector, and the other end of the one-way valve is connected to the Luer double male connector. Connection, the connection mode is tight connection.

Preferably, the culture chamber includes a chamber body formed by tight connection of an upper cover and a lower cover, a culture chamber sample inlet provided on the upper cover and a culture chamber sample outlet provided on the lower cover, the upper The cover is made of transparent material, and when in use, the sample outlet of the culture chamber is connected with the Luer double male connector.

Preferably, a filter membrane, an absorbent pad and a liquid drainage tank are arranged inside the chamber body along the sample flow direction.

Preferably, the air filter further includes a hydrophobic and sterilized air filter membrane and an air filter groove arranged inside the bottom of the air filter.

Preferably, the sampling connector assembly includes a conduit, a three-way valve connector and a sampling connector respectively provided at both ends of the conduit, and the three-way valve connector is connected to the third interface during a sampling operation.

Preferably, the water intake bag is connected to the sampling joint, and a silica gel valve is provided at the joint between the water intake bag and the sampling joint.

Preferably, the medium adding tube adopts a prefilled syringe without a needle and without a push rod.

Preferably, the connection between the Luer double male connector and the sample outlet of the culture chamber, the sample inlet of the culture chamber, the sample outlet of the culture chamber, and the three-way valve connector are all provided with caps through Luer connections , the cap is fastened to the microorganism counting device through a protective rope.

operation method

a. Take out the sampling syringe, one-way valve assembly, culture chamber, three-way valve assembly and sampling joint assembly from the sterilization packaging bag and assemble them in sequence, and take out the water intake bag at the same time;

b. Inject the water sample to be tested into the water intake bag through the silica gel valve, and shake the water intake bag containing the neutralizer;

c. Unplug the protective sleeve of the sampling joint on the sampling joint assembly, and insert the sampling joint into the water bag;

d. Close the second interface of the three-way valve assembly by adjusting the limit valve, open the third interface, then pull the piston of the sampling syringe to a certain scale line, and let it stand for 20-30 seconds to ensure that a sufficient amount of liquid is filtered;

e. Close the third interface and the second interface of the three-way valve assembly by adjusting the limit valve, pull out the sampling joint from the water bag, put it back into the protective sleeve, and remove the sampling joint assembly from the three-way valve assembly;

f. Take out the medium addition tube and auxiliary syringe from the sterilized packaging bag, unscrew the cap plug of the addition tube, and quickly connect to the third interface; unscrew the one-way valve assembly from the sample outlet of the culture chamber, and put it in the culture chamber. The sample outlet is quickly connected to the auxiliary syringe;

g. Open the third interface by adjusting the limit valve, rotate the push rod to the piston of the medium addition tube, and push the push rod to inject the medium into the culture chamber;

h. Close the third interface and open the second interface by adjusting the limit valve, and pull the auxiliary syringe until there is no medium left in the upper cover of the culture chamber;

i. Unscrew the medium addition tube, and use the first cap plug II to close the third interface;

j. Unscrew the auxiliary syringe, and use the second cap plug II to close the sample outlet of the culture chamber;

k. Cultivate the culture bin where the sampling has been completed at different culture temperatures and time according to different microorganisms, and mark the colonies to complete the count.

The technical scheme of the utility model has the following beneficial effects:

1. The microbial counting device of the present utility model includes a sampling syringe, a one-way valve assembly, a training chamber, a three-way valve assembly, a sampling joint assembly and a water intake bag, which are sequentially connected and assembled by the water sample filtering part, and also includes the culture medium adding part of the culture medium part. Tube and auxiliary syringe, in the microgravity non-clean state, the sample passes through the closed sterile channel of the water bag and the sampling joint assembly, is filtered through the filter membrane in the culture chamber, and then injected into the culture medium through the transfer of the three-way valve assembly. And use the auxiliary syringe to filter the excess liquid, and culture and count the microorganisms retained by the filter membrane. The method and design of this device can realize the collection and transfer of samples in a microgravity non-clean environment, and the filtration, cultivation and counting of bacteria and fungi. The process is carried out in a closed state, which not only avoids pollution during the operation process, makes the detection results more accurate, but also effectively prevents internal microorganisms from polluting the external environment during the cultivation process.

2. In the process of microbial counting, the method of pre-setting the absorbent pad under the filter membrane is adopted, and the medium is directly added after filtering the water sample, so as to realize full contact between the microorganism and the medium, without transferring the filter membrane and using a petri dish, reducing pollution way.

3. The use of liquid medium solves the problems that agar will melt when heated and difficult to form under special conditions.

4. The sampling syringe is equipped with a scale and a one-way valve assembly. By pulling out the piston to complete the extraction of a sufficient amount of samples, it cooperates with the one-way valve to control the filtration, which can avoid the downstream pollution intrusion of the sampling syringe before and after operation and the liquid backflow during the operation. In order to avoid operation If the piston is detached during the experimental operation, a limit column should be installed at the end of the sampling syringe.

5. The three joints of the three-way valve can be connected quickly, which is not easy to loosen and has a good sealing effect. The valve has a limited function to avoid misoperation. The three-way valve assembly is used to complete the operation functions of filtering samples, injecting culture medium and drying Switching; the second joint is pre-installed with an air filter whose function is to provide sterile air during the drying process. It uses a hydrophobic and sterilizing air filter membrane inside to prevent the water sample or culture medium from wetting the filter membrane during the filtration process, causing the filter to fail to filter the air. During the filtration process, the air enters the pipeline and the culture chamber through the filter, which can avoid contamination in the air. Microorganisms affect the test results Drain the culture medium and a small amount of residual water samples to avoid microbial growth along the liquid and make it impossible to count.

6. The role of the medium addition tube is to store the liquid medium for a long time, and add the medium to the inside of the culture chamber after the filtration is completed. The medium addition tube adopts a polymer needle-free, push rod-free pre-filled syringe, which avoids glass Risk of medium leakage due to breakage and piston being pushed during violent shocks, prefilled syringes are equipped with a cap at the front end to provide long-term sealed storage.

7. Considering the possibility that the tested water sample contains silver ions, which may inhibit the growth of microorganisms, a certain concentration of neutralizing agent is pre-installed in the water bag to achieve more accurate detection and counting of microorganisms in the water.

8. The sampling joint assembly is a channel for aseptic transfer of liquid in a fully enclosed state. Its function is to achieve a sealed connection with the water intake bag. The protective sleeve protects the water intake bag joint after opening the package, avoiding unnecessary contact and reducing Risk of microbial contamination.

9. The joints of this device are all tightly connected, preferably using Luer connection, which has good sealing performance. Before assembly, each open interface can be equipped with a cap plug through Luer connection. The function of the cap plug is to close the interface, avoiding After the product is taken out, it is contaminated before assembly or during the counting process.

Description of drawings

In order to more clearly illustrate the specific implementation of the utility model or the technical solutions in the prior art, the accompanying drawings that need to be used in the description of the specific implementation or the prior art will be briefly introduced below. Obviously, the following descriptions The accompanying drawings are some implementations of the utility model, and those skilled in the art can also obtain other drawings according to these drawings without any creative work.

Fig. 1 is the structure schematic diagram of the microbial counting device of the present embodiment;

Fig. 2 is a schematic structural diagram of the liquid suction syringe and the one-way valve assembly of the present embodiment;

Fig. 3 is a sectional view of the liquid-absorbing syringe of the present embodiment;

Fig. 4 is the schematic diagram of the structure of the culture chamber of the present embodiment;

Fig. 5 is a schematic structural diagram of the three-way valve assembly of the present embodiment;

Fig. 6 is a schematic structural diagram of the sampling joint assembly of the present embodiment;

Fig. 7 is a schematic diagram of the structure of the medium adding tube in this embodiment.

Explanation of reference signs:

1. Sampling syringe; 11. Sampling inlet; 12. Luer double female connector; 13. Limiting column; 14. Scale; 2. One-way valve assembly; 21. One-way valve; 22. Luer double male connector; 23 , cap plug Ⅰ; 24 protective rope Ⅰ; 3, culture chamber; 31, culture chamber sample inlet; 32, culture chamber sample outlet; 33, chamber body; 331, upper cover; 332, lower cover; 333, filter membrane ; 334, porous pad; 335, liquid drainage groove; 34, the first cap II; 35, fixing piece; 36, protection rope II; 37, the second cap II; Interface; 42, second interface; 43, third interface; 44, limit valve; 45, air filter; 451 air filter membrane; 452, air filter tank; 5, sampling joint assembly; 51, conduit; 52, three Through valve connector; 53 sampling connector; 54, cap plug III; 55, protective cover; 56, protection rope III; 57, protection rope IV; ; 63; piston; 64, push rod; 7, water bag; 71, silica gel valve; 8, auxiliary syringe.

Detailed ways

The technical solutions of the utility model will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are part of the embodiments of the utility model, but not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

In addition, the technical features involved in different embodiments of the present invention described below can be combined with each other as long as they do not constitute conflicts with each other.

Example 1

A microbial counting device as shown in Figure 1, includes a water sample filtering part and an addition medium part, and the water sample filtering part includes a sampling syringe 1, a one-way valve assembly 2, a culture chamber 3, three Through the valve assembly 4, the sampling joint assembly 5 and the water bag 7, the addition of the medium part includes the medium addition tube 6 and the auxiliary syringe 8 used during the medium addition operation.

As shown in Fig. 2 and Fig. 3, the sampling syringe 1 includes a Luer double female connector 12 arranged at the sampling inlet 11, and the sampling syringe 1 also includes a limit stop arranged inside the end of the sampling syringe 1 to prevent the piston from falling off. The column 13 and the scale 14 arranged on the outer surface of the sampling syringe 1 for quantitative detection.

The one-way valve assembly 2 is arranged at the front end of the sampling syringe 1, and includes a one-way valve 21 and a Luer double male connector 22, one end of the one-way valve 21 is connected to the Luer double female connector 12, and the one-way The other end of the valve is connected to the Luer double male connector 22, and the connection methods are all Luer connections supplemented by thread positioning; when not assembled, a pollution-proof cap plug I23 is connected to the Luer double male connector 22, and the cap The plug I23 is bolted on the one-way valve assembly 2 through the protection rope I24 to prevent it from drifting away.

As shown in Figure 4, the culture bin 3 includes a bin body 33 formed by the tight connection of an upper cover 331 and a lower cover 332, a culture bin sample inlet 31 located at the center of the upper cover 331 and a central chamber located at the center of the lower cover 332. The culture bin sample outlet 32, the upper cover is made of transparent material, when in use, the culture bin sample outlet 32 is connected with the Luer double male connector 22, the culture medium inlet 31 is connected with the three The first interface 41 of the valve assembly 4 is connected; when not assembled, the sample inlet 31 of the culture chamber and the sample outlet 32 of the culture chamber are connected with the first cap II 34 and the first cap II 34 of the same structure through a Luer connection. The second cap II, the first cap II34 is bolted to the culture chamber 3 through the protection rope II36, and one end of the protection rope II36 is fixed on the first cap II34 through the fixing part 35;

A filter membrane 333 and an absorbent pad 334 are arranged inside the chamber body 33 along the direction of sample flow, a drainage groove 335 is provided on the inner surface of the lower cover 332, the filter membrane 333 is arranged close to the upper cover 331, and the absorbent pad 334 Set close to the lower cover 332; the liquid drainage groove 335 is a neatly arranged arc-shaped groove, allowing the liquid to flow through quickly and flow out from the sample outlet 32 of the culture chamber.

As shown in Figure 5, the three-way valve assembly 4 includes a first port 41, a second port 42, a third port 43 and a limit valve 44, and the first port 41 is connected to the sample inlet 31 of the culture chamber , the second interface 42 is provided with an air filter 45, the third interface 43 is used to connect the sampling joint assembly 5 or the medium addition tube 6, and the limit valve 44 controls the connection and closure of the three interfaces, The inside of the air filter 45 also includes a hydrophobic and sterilizing air filter membrane 451 and an air filter groove 452 arranged at the bottom of the air filter. The air filter groove 452 can promote the rapid circulation of gas.

As shown in Figure 6, the sampling connector assembly 5 includes a conduit 51, a three-way valve connector 52 and a sampling connector 53 respectively arranged at both ends of the conduit. 43 connection; when not assembled, the three-way valve joint 52 is provided with a cap III54, and the sampling joint is provided with a protective sleeve 55, and the cap III54 and the protective sleeve 55 respectively pass through the protective rope III56 and The protection rope IV57 is bolted on the sampling joint assembly 5 to prevent it from drifting away.

The water intake bag 7 is connected to the sampling joint 53 , and a silica gel valve 71 is provided at the joint between the water intake bag 7 and the sampling joint 53 .

As shown in Figure 7, the medium addition tube 6 adopts a needleless, non-push rod type prefilled syringe 61, the front end is provided with an addition tube cap plug 62, and the rear end is provided with a piston 63 inside, in order to facilitate the introduction of the medium, The medium adding pipe 6 also includes a push rod 64, and when the medium is injected, the push rod 64 acts on the piston 63 to push out the required liquid medium.

The operation method of this embodiment includes the following steps:

a. Take out the sampling syringe 1, the one-way valve assembly 2, the culture chamber 3, the three-way valve assembly 4 and the sampling joint assembly 5 from the sterilized packaging bag and assemble them sequentially, and take out the water intake bag 7 at the same time;

b. Inject the water sample to be tested into the water intake bag 7 through the silica gel valve 71, and shake the water intake bag 7 containing the neutralizing agent;

c. Unplug the protective sleeve 55 of the sampling joint 53 on the sampling joint assembly 5, and insert the sampling joint 53 into the water intake bag 7;

d. Close the second interface 42 of the three-way valve assembly 4 by adjusting the limit valve 44, open the third interface 43, then pull the piston of the sampling syringe 1 to a certain scale line, and let it stand for 20-30 seconds to ensure sufficient liquid is filtered;

e. Close the third interface 43 and the second interface 43 of the three-way valve assembly 4 by adjusting the limit valve 44, pull out the sampling joint 53 from the water bag 7, put back the protective sleeve 55, and remove the sampling joint assembly 5 from the three Remove the through valve assembly 4;

f. Take out the medium addition tube 6 and the auxiliary syringe 8 from the sterilized packaging bag, unscrew the addition tube cap plug 62, and quickly connect to the third interface 43; unscrew the one-way valve assembly 2 from the sample outlet 32 of the culture chamber Next, and quickly connect the auxiliary syringe 8 at the sample outlet 32 of the culture chamber;

g. Open the third interface 43 by adjusting the limit valve 44, rotate the push rod 64 to the piston 63 of the medium addition pipe 6, and push the push rod 64 to inject the medium into the culture chamber 3;

h. Close the third interface 43 and open the second interface 42 by adjusting the limit valve 44, and pull the auxiliary syringe 8 until there is no culture medium residue in the upper cover 331 of the culture chamber 3;

i. Unscrew the medium addition tube 6, and use the first cap plug II 34 to close the third interface 43;

j. Unscrew the auxiliary syringe 8, and use the second cap plug II 37 to close the sample outlet 32 of the culture chamber;

k. Cultivate the culture bin 3 that has been sampled at different culture temperatures and time according to different microorganisms, and count the colonies.

Apparently, the above-mentioned embodiments are only examples for clear description, rather than limiting the implementation. For those of ordinary skill in the art, on the basis of the above description, other changes or changes in different forms can also be made. It is not necessary and impossible to exhaustively list all the implementation manners here. And the obvious changes or changes derived therefrom are still within the scope of protection of the utility model.

Claims (10)

1. A microbial counting device can be divided into a water sample filter part and an added culture medium part according to the operation process, it is characterized in that, The water sample filtering part includes a sampling syringe (1) which is fixedly connected and assembled sequentially during use, a one-way valve assembly (2) arranged at the front end of the sampling syringe (1), a culture chamber (3), a three-way valve assembly (4), a sampling A joint assembly (5) and a water intake bag (7), the two ends of the culture bin (3) are provided with a culture bin sample inlet (31) and a culture bin sample outlet (32), and the three-way valve assembly (4) Including a first interface (41), a second interface (42), a third interface (43) and a limit valve (44), the first interface (41) is connected to the culture chamber sample inlet (31), An air filter (45) is provided on the second interface (42); The medium addition part also includes a medium addition tube (6) and an auxiliary syringe (8) used during the medium addition operation. 2. microorganism counting device according to claim 1, is characterized in that, described sampling injector (1) comprises, is located at the Luer double female connector (12) of sampling inlet (11) place; It also includes a limit column (13) arranged inside the end of the sampling syringe (1) and a scale (14) arranged on the outer surface of the sampling syringe (1). 3. microorganism counting device according to claim 1 or 2, is characterized in that, described one-way valve assembly (2) comprises one-way valve (21) and Luer double male joint (22), and described one-way valve ( One end of 21) is connected to the Luer double female connector (12), and the other end of the check valve (21) is connected to the Luer double male connector (22), all of which are tightly connected. 4. The microorganism counting device according to claim 3, characterized in that, the culture bin (3) comprises a bin body (33) formed by tight connection of an upper cover (331) and a lower cover (332), and is arranged on the The culture chamber sample inlet (31) of the above-mentioned loam cake (331) and the culture chamber sample outlet (32) located at the described lower cover (332), the described loam cake (331) is a transparent material, during use, the The sample outlet (32) of the culture chamber is connected to the Luer double male connector (22). 5 . The microorganism counting device according to claim 4 , characterized in that, a filter membrane ( 333 ), an absorbent pad ( 334 ) and a drain tank ( 335 ) are arranged inside the chamber body ( 33 ) along the sample flow direction. 6. according to claim 1 or 2 or 4 described microbial counting devices, it is characterized in that, also comprise the air filter membrane (451) of hydrophobic sterilization in the described air filter (45) and be located at described air filter Air filter slot (452) on the inside of the bottom. 7. The microorganism counting device according to claim 1, 2 or 4, characterized in that, the sampling joint assembly (5) comprises a conduit (51), a three-way valve joint (52) and a sampling joint which are respectively arranged at two ends of the conduit. A joint (53), the three-way valve joint (52) is connected with the third interface (43) when performing a sampling operation. 8. The microbial counting device according to claim 7, characterized in that, the water intake bag (7) is connected to the sampling joint (53), and the water intake bag (7) is connected to the sampling joint (53) Place is provided with a silica gel valve (71). 9. The microorganism counting device according to claim 1, characterized in that, the medium adding tube (6) adopts a prefilled syringe (61) without a needle and without a push rod. 10. microorganism counting device according to claim 3, is characterized in that, described Luer double male connector (22) and culture bin sample inlet (31) joint, described culture bin sample outlet (32), the The sample inlet (31) of the culture chamber and the three-way valve joint (52) are all provided with a cap through a Luer connection, and the cap is tied to the microorganism counting device through a protective rope.