CN204661695U - A kind of sampling unit detecting fermentative production sterile air - Google Patents
A kind of sampling unit detecting fermentative production sterile air Download PDFInfo
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- CN204661695U CN204661695U CN201520105859.9U CN201520105859U CN204661695U CN 204661695 U CN204661695 U CN 204661695U CN 201520105859 U CN201520105859 U CN 201520105859U CN 204661695 U CN204661695 U CN 204661695U
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Abstract
The utility model belongs to the technical field of fermentative production, is specifically related to a kind of sampling unit detecting fermentative production sterile air.This kind detects the sampling unit of fermentative production sterile air, the sampling jug comprising pipe connecting and be connected with pipe connecting; The port of described pipe connecting one end is connected with sterile air control valve, and the upside of the pipe connecting the other end is provided with steam admission control valve, and the downside of this end of pipe connecting is provided with steam and goes out vapour control valve; Go out between vapour control valve to be provided with tensimeter at steam admission control valve and steam; The bottle mouth position of described sampling jug is respectively equipped with silicone tube and nuclepore membrane filter; Culture medium layer is provided with in described sampling jug.This sampling unit eliminates the miscellaneous bacteria interference in sampling process, and science reasonable in design is simple to operation, and the sterile air be applicable in fermentable production detects sampling, has great promotional value.
Description
Technical field
The utility model belongs to the technical field of fermentative production, is specifically related to a kind of sampling unit detecting fermentative production sterile air.
Background technology
In microorganism aerobic fermenting process, sterile air is needed to provide oxygen for microorganism.Current industrial acquisition fermentation sterile air generally first adopts high pressure steam to carry out sterilising treatment to air line and air filter, then adopts air filter filtering compressed air to obtain sterile air; But there is no science detect sampling unit accurately for whether sterile air used in identification and detection fermentative production qualified.If cannot science whether detect obtained sterile air accurately qualified, then can bring huge financial loss to fermentative production; When air system microbiological contamination is serious, will cause fermentation enterprise tank switching, be even forced to stop production, this phenomenon is of common occurrence, brings about great losses to fermentation enterprise.
Summary of the invention
The purpose of this utility model is for science precisely cannot detect the whether qualified problem of obtained sterile air and provide a kind of sampling unit detecting fermentative production sterile air at present.This sampling unit eliminates the miscellaneous bacteria interference in sampling process, and science reasonable in design is simple to operation, and the sterile air be applicable in fermentable production detects sampling, has great promotional value.
The technical solution of the utility model is: a kind of sampling unit detecting fermentative production sterile air, the sampling jug comprising pipe connecting and be connected with pipe connecting; The port of described pipe connecting one end is connected with sterile air control valve, and the upside of the pipe connecting the other end is provided with steam admission control valve, and the downside of this end of pipe connecting is provided with steam and goes out vapour control valve; Go out between vapour control valve to be provided with tensimeter at steam admission control valve and steam; The bottle mouth position of described sampling jug is respectively equipped with silicone tube and nuclepore membrane filter; Culture medium layer is provided with in described sampling jug.
Its material of described pipe connecting is stainless steel.
One end of described silicone tube is positioned at the below of culture medium layer.
Described silicone tube is positioned on one end tube wall below culture medium layer and is evenly provided with hole and the port sealing of this end.
Described sampling jug is provided with bottle cap, and this bottle cap is provided with supporting tube I and supporting tube II; The wherein bottle inner port socket silicone tube of supporting tube I, the bottle external port of this supporting tube I is socketed a flexible pipe, and this flexible pipe the other end port is provided with sampling jug air inlet valve; The bottle external port socket nuclepore membrane filter of supporting tube II.
Described sampling jug is flexibly connected with pipe connecting.
The beneficial effects of the utility model are: the sampling unit reasonable in design of detection fermentative production sterile air described in the utility model, first the sampling jug after high-temp steam sterilizing is connected with pipe connecting, high-temperature steam goes out vapour control valve pair by steam admission control valve and steam and pipe connecting carries out steam sterilizing process, removes the pollution of miscellaneous bacteria; This sampling unit can be ventilated continuously; In sampling jug, culture medium layer can be used for microorganism culturing, and sampling result has science and representativeness; Adopt adjustable gate, conservative control air input simultaneously, air is slowly entered, prevent vial and pipeline burst, ensure sampling process security; Shaking bath is put into or incubator is cultivated after sampling jug is detachable.
This sampling unit eliminates the miscellaneous bacteria interference in sampling process, and science reasonable in design is simple to operation, and the sterile air be applicable in fermentable production detects sampling, has great promotional value.
Accompanying drawing explanation
Fig. 1 is the structural representation of the sampling unit detecting fermentative production sterile air in the utility model embodiment.
Wherein, 1 is sterile air control valve, and 2 is tensimeter, and 3 is steam admission control valve, and 4 go out vapour control valve for steam, 5 is sampling jug air inlet valve, and 6 is sampling jug, and 7 is supporting tube I, and 8 is supporting tube II, 9 is nuclepore membrane filter, and 10 is silicone tube, and 11 is pipe connecting, and 12 is flexible pipe.
Embodiment
Below in conjunction with accompanying drawing, the technical solution of the utility model is described in detail.
As can be seen from Figure 1, a kind of sampling unit detecting fermentative production sterile air of the present utility model, the sampling jug 6 comprising pipe connecting 11 and be connected with pipe connecting 11; The port of described pipe connecting 11 one end is connected with sterile air control valve 1, and the upside of pipe connecting 11 the other end is provided with steam admission control valve 3, and the downside of this end of pipe connecting 11 is provided with steam and goes out vapour control valve 4; Go out between vapour control valve 4 to be provided with tensimeter 2 at steam admission control valve 3 and steam; The bottle mouth position of described sampling jug 6 is respectively equipped with silicone tube 10 and nuclepore membrane filter 9; Culture medium layer is provided with in described sampling jug 6.Wherein one end of silicone tube 10 is positioned at the below of culture medium layer.This silicone tube 10 is positioned on one end tube wall below culture medium layer and is evenly provided with hole and the port sealing of this end.
Described sampling jug 6 is provided with bottle cap, and what bottle cap was provided with through bottle cap is provided with supporting tube I 7 and supporting tube II 8; The wherein bottle inner port socket silicone tube 10 of supporting tube I 7, the bottle external port of this supporting tube I 7 is socketed a flexible pipe 12, and this flexible pipe 12 the other end port is provided with sampling jug air inlet valve 5; The bottle external port socket nuclepore membrane filter 9 of supporting tube II 8.Described sampling jug 6 is flexibly connected with pipe connecting 11.
Stainless steel pipe connecting 11 is connected with the escape pipe of air filter by sterile air control valve 1, substratum will be housed and sampling jug 6 after high-temperature sterilization process is connected with pipe connecting 11 by the sampling jug air inlet valve 5 of bottle outer flexible tube 12 port; Close sterile air control valve 1, steam admission control valve 3 is accessed high-temperature steam, according to tensimeter 2 displayed value, steam regulation admission control valve 3 and steam go out vapour control valve 4 pairs of stainless steel pipe connectings 11 and carry out steam sterilizing, remove living contaminants problem in connection procedure.Sterile air control valve 1 is opened after sterilizing terminates, and steam off admission control valve 3, after stainless steel pipe connecting 11 temperature is down to room temperature, steam off goes out vapour control valve 4, and open sampling jug air inlet valve 5 gradually, according to the displayed value of tensimeter 2, reasonable adjusting sterile air control valve 1 and sampling jug air inlet valve 5, make gas progress into sampling jug 6.After sampling terminates, close sampling jug air inlet valve 5, by crack for sterile air control valve 1, and sampling jug 6 is disassembled by Link Port between pipe connecting 11 and sampling jug air inlet valve 5, sampling jug 6 is put into shaking bath or incubator continuation cultivation.
Claims (6)
1. detect a sampling unit for fermentative production sterile air, it is characterized in that, the sampling jug comprising pipe connecting and be connected with pipe connecting; The port of described pipe connecting one end is connected with sterile air control valve, and the upside of the pipe connecting the other end is provided with steam admission control valve, and the downside of this end of pipe connecting is provided with steam and goes out vapour control valve; Go out between vapour control valve to be provided with tensimeter at steam admission control valve and steam; The bottle mouth position of described sampling jug is respectively equipped with silicone tube and nuclepore membrane filter; Culture medium layer is provided with in described sampling jug.
2. detect the sampling unit of fermentative production sterile air according to claim 1, it is characterized in that, its material of described pipe connecting is stainless steel.
3. detect the sampling unit of fermentative production sterile air according to claim 1, it is characterized in that, one end of described silicone tube is positioned at the below of culture medium layer.
4. detect the sampling unit of fermentative production sterile air according to claim 3, it is characterized in that, described silicone tube is positioned on one end tube wall below culture medium layer and is evenly provided with hole and the port sealing of this end.
5. detect the sampling unit of fermentative production sterile air according to claim 1, it is characterized in that, described sampling jug is provided with bottle cap, and this bottle cap is provided with supporting tube I and supporting tube II; The wherein bottle inner port socket silicone tube of supporting tube I, the bottle external port of this supporting tube I is socketed a flexible pipe, and this flexible pipe the other end port is provided with sampling jug air inlet valve; The bottle external port socket nuclepore membrane filter of supporting tube II.
6. detect the sampling unit of fermentative production sterile air according to claim 1, it is characterized in that, described sampling jug is flexibly connected with pipe connecting.
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CN201520105859.9U CN204661695U (en) | 2015-02-13 | 2015-02-13 | A kind of sampling unit detecting fermentative production sterile air |
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CN201520105859.9U CN204661695U (en) | 2015-02-13 | 2015-02-13 | A kind of sampling unit detecting fermentative production sterile air |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109628289A (en) * | 2019-01-03 | 2019-04-16 | 伊犁川宁生物技术有限公司 | The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique |
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2015
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109628289A (en) * | 2019-01-03 | 2019-04-16 | 伊犁川宁生物技术有限公司 | The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique |
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