CN107216996A - A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation - Google Patents
A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation Download PDFInfo
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- CN107216996A CN107216996A CN201710376353.5A CN201710376353A CN107216996A CN 107216996 A CN107216996 A CN 107216996A CN 201710376353 A CN201710376353 A CN 201710376353A CN 107216996 A CN107216996 A CN 107216996A
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- seeding tank
- measurable
- bottle
- injection needle
- classification inoculation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention belongs to technical field of biological fermentation, and in particular to a kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for being used to ferment for utilizing the device.The device includes being used for the seed bottle for containing ripe bacterium solution and the peristaltic pump being connected with seed bottle；The seed bottle includes body and sealed bottle cap two parts, and wherein bottle cap is provided with two hollow pipes stretched into bottle, wherein the first hollow pipe stretches into bottom of bottle；First hollow pipe, after being connected by the first connecting tube with peristaltic pump, further passes through the second connecting tube（For example, silicone tube）Injection needle is connected with, injection needle is stretched into seeding tank.The application can preferably ensure convenience, security and validity in operating process, mechanization, modernization, cost degradation for inoculation operation have preferable application value, while sterileization also for fermentation process and ensuring that ferment effect stability is provided and preferably ensureing.
Description
Technical field
The invention belongs to technical field of biological fermentation, and in particular to a kind of measurable classification inoculation apparatus of seeding tank and utilize the dress
The measurable inoculation method for being used to ferment put.
Background technology
In biological fermentation process, to ensure relatively stable ferment effect, the precision and the degree of accuracy for inoculum concentration are general
It there are certain requirements.In actual mechanical process, to avoid miscellaneous bacteria from infecting, typically more conventional inoculation method for flame inoculation method and
, although both approaches require relatively low for inoculating facility, there is the control that inoculum concentration is difficult in pressure difference inocalation method
Defect, so that it is certain uncertain to cause ferment effect to exist, and both inoculation methods are for small-sized fermentation equipment application
When it is also not convenient enough, thus pole be necessary to develop it is a kind of more easily, be easy to control and adjust the classification inoculation apparatus of inoculum concentration, from
And provide preferable guarantee for the stability of ferment effect.
The content of the invention
Measurable classification inoculation apparatus when the application main purpose is to provide a kind of inoculation for seeding tank, consequently facilitating connecing
The regulation and control of liquid inoculum concentration are planted, and then are provided safeguard for the stable of fermentation process.
Details are as follows for the technical scheme that the application is taken.
A kind of measurable classification inoculation apparatus of seeding tank, the device includes：For containing ripe bacterium solution（Inoculation seed liquor）'s
Seed bottle and the peristaltic pump being connected with seed bottle；
The seed bottle includes body and the bottle cap two parts being tightly connected with body, and wherein bottle cap is stretched into bottle provided with two
Hollow pipe, wherein the first hollow pipe stretches into bottom of bottle, another second hollow pipe, which is only stretched into bottle, to be used to ventilate（To balance seed
The inside and outside differential pressure of bottle）；The hollow pipe material uses high temperature high voltage resistant, corrosion-resistant material, for example with metal tube, specifically for example
For stainless steel tube；
Second hollow pipe stretches out the end of bottle cap, is preferably connected with filtration core, was used for air filtering（For example with small size air
Gas essence filtration core）, to ensure that miscellaneous bacteria will not pollute bacterium solution in seed bottle in air；
First hollow pipe, passes through the first connecting tube（For example, silicone tube）After being connected with peristaltic pump, the first connecting tube is close
The end of peristaltic pump passes through the second connecting tube（For example, silicone tube）Injection needle is connected with, injection needle is stretched into seeding tank；
In being preferably provided with, liquid stopping clamp is provided between the first connecting tube and peristaltic pump, is flowed when needed with ensuring against liquid；
It is preferred that in connected mode, injection needle use used on screw thread injection needle, seeding tank with injection needle it is supporting containing micro-
Hole（For passing through syringe needle）Threaded fastener, replaceable sealing gasket is preferably provided between screw thread injection needle and fastener
（Specific such as silicagel pad）, injection needle is connected by fastener with seeding tank；The fastener is, for example, specifically：Containing interior tyre
The hollow bolt of screw thread and with the compact union (screwed)bonnet (UB of hollow bolt（Union (screwed)bonnet (UB is used to be tightly connected with seeding tank）, spiral shell
The external screw thread of line injection needle and hollow bolt is closely connected by screw thread.
During concrete application, because injection needle is not required to be continuously connected with seeding tank, thus it is preferably provided with, outside injection needle
Portion is provided with supporting protection device, when lacking supporting protection device, specifically for example sets as follows, to avoid syringe needle from damaging
Lose：If being provided with dried layer gauze outside syringe needle, wrapped up again using brown paper outside gauze, and tightened with cotton thread.
Using the measurable inoculation method for being used to ferment of the measurable classification inoculation apparatus of the seeding tank, this method is relatively applicable
The liquid spawn inoculation of Minitype seed tank in production process is used, and specifically includes following steps：
（1）Seed bottle in the measurable classification inoculation apparatus of seeding tank is sterilized, after sterilizing, in bacterium inoculation area into seed bottle
Use seed liquor to be seeded is poured into, and in peristaltic pump connection；
（2）By injection needle after sterilizing from stretching into seeding tank（The measurable classification inoculation apparatus connection of seeding tank is completed）；Open compacted
Dynamic pump, the seed liquor volume in seeding tank is inoculated into by wriggling pump rate and time control.
The present invention's mainly has the beneficial effect that following two aspects：
1st, because the speed of peristaltic pump is relatively slow, thus preferably can accurately it be controlled for inoculum concentration, while being easy to machinery
Change operation, improve operating efficiency, while the influence of manual operation error can be reduced preferably；
2nd, classification inoculation apparatus and inoculation method provided herein, with preferable security, microbiological contamination probability is small, so that it is guaranteed that preferably connecing
Plant effect.
In general, classification inoculation apparatus provided herein, novel in design, it is convenient in operating process preferably to ensure
Property, security and validity, there is preferable application value for the mechanization, modernization, cost degradation of inoculation operation, simultaneously
Also sterileization for fermentation process and ensure that ferment effect stability provides preferable guarantee, for improving production efficiency and guarantee
Product quality has preferable practical value and popularization and application meaning.
Brief description of the drawings
Fig. 1 is the structural representation of seed bottle in the measurable classification inoculation apparatus of seeding tank provided herein；
Fig. 2 is seeding tank top structure schematic diagram in the measurable classification inoculation apparatus of seeding tank provided herein；
Fig. 3 is the overall connection diagram of the measurable classification inoculation apparatus of seeding tank provided herein.
Embodiment
Explanation is further explained to the application with reference to the accompanying drawings and examples.
Embodiment
As shown in Fig. 1 ~ 3, the measurable classification inoculation apparatus of seeding tank provided herein, the device includes：For containing into
Ripe bacterium solution（Inoculation seed liquor）Seed bottle 1 and with the peristaltic pump 2 that is connected of seed bottle；
Seed bottle 1 includes body 3 and the two parts of sealed bottle cap 4, wherein bottle cap provided with two stretch into bottle it is hollow
Pipe（First hollow pipe 5 and the second hollow pipe 6）, wherein the first hollow pipe 5 stretches into bottom of bottle, another second hollow pipe 6 is only stretched into
It is used to ventilate in bottle（To balance the inside and outside differential pressure of seed bottle）；The hollow pipe material uses high temperature high voltage resistant, corrosion-resistant material,
For example with metal tube, specific for example, stainless steel tube；
Second hollow pipe 6 stretches out bottle cap end, is preferably connected with filtration core 7, was used for air filtering（For example with small size air
Gas essence filtration core）, to ensure that miscellaneous bacteria will not pollute bacterium solution in seed bottle in air；
First hollow pipe 5, passes through the first connecting tube 8（For example, silicone tube）After being connected with peristaltic pump 2, further pass through
Two connecting tubes 9（For example, silicone tube）Injection needle 10 is connected with, injection needle is stretched into seeding tank 11；
In being preferably provided with, liquid stopping clamp 12 is provided between the first connecting tube 8 and peristaltic pump 2, is flowed when needed with ensuring against liquid；
It is preferred that in connected mode, injection needle 10 is used on screw thread injection needle, seeding tank 11 using supporting containing micropore（For
Pass through syringe needle）Threaded fastener, replaceable sealing gasket is preferably provided between screw thread injection needle and fastener（Concrete example
Such as silicagel pad）；The fastener is, for example, specifically：Containing inside and outside threaded hollow bolt and with the compact spiral shell of hollow bolt
Line gland（Union (screwed)bonnet (UB is used to be tightly connected with seeding tank）, the external screw thread of screw thread injection needle and hollow bolt is tight by screw thread
Close connection.
During concrete application, because injection needle 10 is not required to be continuously connected with seeding tank 11, thus it is preferably provided with, injection needle
Head is outside to be provided with supporting protection device, when lacking supporting protection device, specifically for example sets as follows, to avoid pin
Head loss：If being provided with dried layer gauze outside syringe needle, wrapped up again using brown paper outside gauze, and tightened with cotton thread.
The seeding tank 11, exemplified by the 10L Minitype seed tanks of certain brand are commonly used in production, its top is typically provided with inoculation
Mouthfuls 13, charging aperture 14 and air vent 15, as example, during concrete application classification inoculation apparatus provided herein, its application method is such as
Under.
（1）First, seed bottle 1 is sterilized（121 DEG C of holding 15min）, sterilize and dry in the air to room temperature, preferable case
Under, first connecting tube 8 and peristaltic pump 2 are closed with liquid stopping clamp 12, to ensure that bacterium solution will not be random before follow-up formal inoculation
Flowing, then pours into seed bottle 1 in aseptic inoculation area and to be seeded uses seed liquor；
（2）Before formal inoculation, by injection needle after sterilizing 10（When having protection device, injection needle is entered simultaneously with protection device
Row sterilizing, consequently facilitating ensuring the aseptic of injection needle）In the inoculation mouth 13 for stretching into the top of seeding tank 11（Thing in seeding tank 11
Charging aperture 14 is first passed through to feed and sterilize；It should be noted that when syringe needle 10 is stretched into seeding tank 11, should aseptically enter
OK, specifically for example, under flame protection, injection needle 10 is passed through into inoculation mouth 13）；Peristaltic pump 2 is opened, passes through wriggling pump rate
The seed liquor volume in seeding tank 11 is inoculated into opening time control；Reach after the scheduled time, close peristaltic pump 2.
In general, in classification inoculation apparatus provided herein, it can form preferably sterile between seed bottle 1 and seeding tank 11
Isolation environment passage, and then seed liquor to be seeded is realized flowing using the power of peristaltic pump 2, while being easy to control to be inoculated with
Amount.Using said apparatus and inoculation method, to some bacterial strains（Such as bacillus subtilis, saccharomycete）Inoculation experiments in,
The safety of inoculation liquid and quantitative flowing preferably are realized, preferable ferment effect, the stability of ferment effect is achieved
Preferable lifting and guarantee have been obtained, has provided for subsequent product increased quality and stably and preferably ensures.
Claims (9)
1. a kind of measurable classification inoculation apparatus of seeding tank, it is characterised in that the device includes：Seed for containing ripe bacterium solution
Bottle, the peristaltic pump and seeding tank being connected with seed bottle；
Seed bottle includes body and the bottle cap being tightly connected with body, wherein bottle cap be provided with two stretch into bottle first
Hollow pipe and the second hollow pipe, wherein the first hollow pipe stretches into bottom of bottle；
First hollow pipe, after being connected by the first connecting tube with peristaltic pump, is further connected with note by the second connecting tube
Syringe needle is penetrated, injection needle is stretched into seeding tank.
2. the measurable classification inoculation apparatus of seeding tank as claimed in claim 1, it is characterised in that the hollow pipe material is that high temperature resistant is high
Pressure, corrosion-resistant material.
3. the measurable classification inoculation apparatus of seeding tank as claimed in claim 2, it is characterised in that the hollow pipe material is stainless steel.
4. the measurable classification inoculation apparatus of seeding tank as claimed in claim 1, it is characterised in that the second hollow pipe stretches out bottle cap end and connected
It is connected to filtration core.
5. the measurable classification inoculation apparatus of seeding tank as claimed in claim 1, it is characterised in that be provided between the first connecting tube and peristaltic pump
Liquid stopping clamp.
6. the measurable classification inoculation apparatus of seeding tank as claimed in claim 1, it is characterised in that injection needle uses screw thread injection needle
Head, seeding tank is provided with the fastener supporting with injection needle, and injection needle is connected by fastener with seeding tank.
7. the measurable classification inoculation apparatus of seeding tank as claimed in claim 6, it is characterised in that the fastener is：Containing interior tyre spiral shell
The hollow bolt of line and with the compact union (screwed)bonnet (UB of hollow bolt, the external screw thread of screw thread injection needle and hollow bolt passes through
Screw thread is closely connected.
8. the measurable classification inoculation apparatus of seeding tank as claimed in claim 1, it is characterised in that supporting guarantor is provided with outside injection needle
Protection unit.
9. utilize the measurable inoculation side for being used to ferment of any one of the claim 1 ~ 8 measurable classification inoculation apparatus of seeding tank
Method, it is characterised in that specifically include following steps：
（1）Seed bottle in the measurable classification inoculation apparatus of seeding tank is sterilized, after sterilizing, in bacterium inoculation area into seed bottle
Pour into and to be seeded use seed liquor；Then the measurable classification inoculation apparatus connection of seeding tank is completed；
（2）Peristaltic pump is opened, the seed liquor volume in seeding tank is inoculated into by wriggling pump rate and time control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710376353.5A CN107216996A (en) | 2017-05-25 | 2017-05-25 | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710376353.5A CN107216996A (en) | 2017-05-25 | 2017-05-25 | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation |
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| Publication Number | Publication Date |
|---|---|
| CN107216996A true CN107216996A (en) | 2017-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710376353.5A Pending CN107216996A (en) | 2017-05-25 | 2017-05-25 | A kind of measurable classification inoculation apparatus of seeding tank and the measurable inoculation method for fermentation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172753A (en) * | 2016-08-23 | 2016-12-07 | 山西维尔生物乳制品有限公司 | A kind of liquid fermentation agent classification inoculation apparatus and method |
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
| CN110628590A (en) * | 2018-06-22 | 2019-12-31 | 深圳市前海小藻科技有限公司 | Fermentation method for reducing risk of bacterial contamination |
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| US3960670A (en) * | 1975-09-02 | 1976-06-01 | Pflug Irving J | Method and apparatus for sterility monitoring |
| CN203001481U (en) * | 2012-12-19 | 2013-06-19 | 无锡江原安迪科分子核医学研究发展有限公司 | Multi-synthesis module for preparing radiopharmaceuticals |
| CN203794896U (en) * | 2014-03-24 | 2014-08-27 | 洛阳华荣生物技术有限公司 | Fermentation tank inoculation device |
| CN104560693A (en) * | 2015-01-29 | 2015-04-29 | 浦城正大生化有限公司 | High-capacity bacterial liquid inoculating device and differential pressure injection inoculation method by use of device |
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2017
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3960670A (en) * | 1975-09-02 | 1976-06-01 | Pflug Irving J | Method and apparatus for sterility monitoring |
| CN203001481U (en) * | 2012-12-19 | 2013-06-19 | 无锡江原安迪科分子核医学研究发展有限公司 | Multi-synthesis module for preparing radiopharmaceuticals |
| CN203794896U (en) * | 2014-03-24 | 2014-08-27 | 洛阳华荣生物技术有限公司 | Fermentation tank inoculation device |
| CN104560693A (en) * | 2015-01-29 | 2015-04-29 | 浦城正大生化有限公司 | High-capacity bacterial liquid inoculating device and differential pressure injection inoculation method by use of device |
Non-Patent Citations (1)
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| 张胜友: "《新法栽培姬菇》", 28 February 2010 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172753A (en) * | 2016-08-23 | 2016-12-07 | 山西维尔生物乳制品有限公司 | A kind of liquid fermentation agent classification inoculation apparatus and method |
| CN108384708A (en) * | 2018-05-23 | 2018-08-10 | 宁夏西麒麟生物科技有限公司 | A kind of sterile controlled amounts type classification inoculation apparatus of seeding tank and the sterile controlled amounts type inoculation method for fermenting or cultivating |
| CN110628590A (en) * | 2018-06-22 | 2019-12-31 | 深圳市前海小藻科技有限公司 | Fermentation method for reducing risk of bacterial contamination |
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Application publication date: 20170929 |