CN212199248U - Continuous inoculation device - Google Patents
Continuous inoculation device Download PDFInfo
- Publication number
- CN212199248U CN212199248U CN202020583106.XU CN202020583106U CN212199248U CN 212199248 U CN212199248 U CN 212199248U CN 202020583106 U CN202020583106 U CN 202020583106U CN 212199248 U CN212199248 U CN 212199248U
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- Prior art keywords
- inoculation
- bottle
- hood
- stop valve
- pipe
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- 238000011081 inoculation Methods 0.000 title claims abstract description 124
- 230000003115 biocidal effect Effects 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 4
- 239000010935 stainless steel Substances 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 15
- WLNBMPZUVDTASE-HXIISURNSA-N (2r,3r,4s,5r)-2-amino-3,4,5,6-tetrahydroxyhexanal;sulfuric acid Chemical compound [O-]S([O-])(=O)=O.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO.O=C[C@H]([NH3+])[C@@H](O)[C@H](O)[C@H](O)CO WLNBMPZUVDTASE-HXIISURNSA-N 0.000 abstract description 9
- 239000001963 growth medium Substances 0.000 abstract description 8
- 239000012530 fluid Substances 0.000 abstract description 3
- 238000001802 infusion Methods 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 description 12
- 239000007788 liquid Substances 0.000 description 10
- 238000010899 nucleation Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
The utility model aims to provide a can realize continuous inoculation device of culture medium fluid infusion, inoculation bottle disinfection and inoculation seamless connection, including inoculation hood, inoculation bottle, dona tub and inoculation valve, the open-top of inoculation bottle, inoculation hood threaded connection is in the open-top of inoculation bottle, the last exhaust hole that has seted up of inoculation hood, the inoculation valve is installed between inoculation bottle and the dona tub, the inoculation valve is three-way antibiotic stop valve, main siphunculus both ends on the three-way antibiotic stop valve are connected with dona tub, an inoculation bottle respectively, the bypass pipe and the steam pipe of three-way antibiotic stop valve are connected, and when the stop valve rod was closed, the bypass pipe can communicate with the inoculation bottle, the mouth of pipe at main siphunculus both ends is connected with dona tub, an inoculation bottle through flange respectively, the inoculation bottle is made for stainless steel material, and a stress application rod is fixedly arranged on the inoculation hood.
Description
Technical Field
The utility model relates to a microorganism seed fermentation field specifically is a continuous inoculation device.
Background
In the fermentation production, microbial seeds are firstly cultured in an aseptic environment and then put into an aseptic container, and when the seeding tank is put into a culture medium for disinfection and cooling to the temperature required by the process, the seeds are inoculated into the seeding tank, and the process is called inoculation. Among the prior art, the utility model patent of patent number 201320416729.8 discloses an inoculation device, including the inoculation bottle, through the screw thread with the bottle lid that the bottleneck at inoculation bottle top is connected and with the inoculation valve that the inoculation mouth of inoculation bottle bottom links to each other, be equipped with the silica gel packing ring in the bottle lid, when screwing up during the bottle lid, the silica gel packing ring is used for the bottleneck of sealed inoculation bottle, be provided with the exhaust hole on the bottle lid, the exhaust hole is located near the lower limb of silica gel packing ring. The inoculation device provided by the utility model has simple structure and easy operation, and the steam is discharged from the exhaust holes on the bottle cap of the inoculation bottle during sterilization, thereby ensuring the fluidity of the steam, eliminating the dead angle of the equipment and further ensuring the thoroughness of sterilization; during inoculation, a closed inoculation fire ring is adopted, liquid is poured into an inoculation bottle under flame, then an inoculation bottle cap is screwed down, the liquid naturally flows into a seed tank, and meanwhile, the fire ring is removed, so that the sterilization effect and the safety of inoculation operation are further ensured.
In the inoculation process of the inoculation device, when the inoculation bottle is disinfected, the inoculation valve is opened, the inoculation bottle and the seed tank are in a communicated state, high-temperature steam is introduced from the seed tank and flows through the inoculation bottle and then flows out from the exhaust hole in the bottle cap, after the disinfection is finished, seed liquid is poured into the inoculation bottle, after the seed tank is cultured and moved away, the tank body is cleaned, a prepared culture medium is injected, and the next round of inoculation is carried out after the whole inoculation is disinfected again.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a can realize continuous inoculation device of culture medium fluid infusion, inoculation bottle disinfection and inoculation seamless connection to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
a continuous inoculation device comprises an inoculation hood, an inoculation bottle, a seed tank and an inoculation valve, wherein the top of the inoculation bottle is open, the inoculation hood is in threaded connection with the top of the inoculation bottle, an exhaust hole is formed in the inoculation hood, the inoculation valve is installed between the inoculation bottle and the seed tank, the inoculation valve is a three-way antibiotic stop valve, two ends of a main through pipe on the three-way antibiotic stop valve are respectively connected with the seed tank and the inoculation bottle, a bypass pipe of the three-way antibiotic stop valve is connected with a steam pipe, and when a stop valve rod is closed, the bypass pipe can be communicated with the inoculation bottle.
As a further aspect of the present invention: the pipe orifices at the two ends of the main through pipe are respectively connected with the seed tank and the inoculation bottle through connecting flanges.
As a further aspect of the present invention: the inoculation bottle is made of stainless steel materials.
As a further aspect of the present invention: and a stress application rod is fixedly arranged on the inoculation hood.
As a further aspect of the present invention: the periphery of inoculation bottle just is located and is close to inoculation hood department fixed mounting has the tray fire.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses simple structure, convenient to use through the setting of tee bend antibiotic stop valve to main siphunculus both ends on this tee bend antibiotic stop valve are connected with dona tub, inoculation bottle respectively, the bypass pipe and the steam pipe of tee bend antibiotic stop valve are connected, and when the stop valve rod was closed, the bypass pipe can communicate with the inoculation bottle to make the dona tub realize under sealed pressurize state that the individual sterilization of inoculation bottle, and then can make the process of inoculation bottle sterilization, notes liquid and the process that the dona tub walked liquid fluid infusion go on in step, thereby realize the dona tub and vaccinate in succession, and then realize serialization production, reduce the steam use amount simultaneously, reduce the energy consumption by a wide margin.
Drawings
FIG. 1 is a schematic view of a continuous inoculating device;
FIG. 2 is an enlarged view of a portion of the continuous inoculating device;
in the figure: the device comprises a forcing rod 1, an inoculation hood 2, an exhaust hole 3, a fire tray 4, an inoculation bottle 5, a steam pipe 6, a bypass valve rod 7, a bypass pipe 8, a stop valve rod 9, a three-way antibiotic stop valve 10, a seed tank 11 and a connecting flange 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, in an embodiment of the present invention, a continuous inoculation device includes an inoculation hood 2, an inoculation bottle 5, a seed tank 11 and an inoculation valve, the top opening of the inoculation bottle 5 is provided, the inoculation hood 2 is screwed on the top opening of the inoculation bottle 5, the inoculation hood 2 is provided with an exhaust hole 3, the inoculation valve is installed between the inoculation bottle 5 and the seed tank 11, the inoculation valve is a three-way antibiotic stop valve 10, the three-way antibiotic stop valve 10 is provided with a main through pipe and a bypass pipe 8, wherein the main through pipe is provided with a stop valve plate and is driven by a stop valve rod 9 to open and close the stop valve plate so as to open and close the main through pipe, the bypass pipe 8 is installed on the main through pipe and is located at one side of the stop valve plate, the bypass pipe 8 is provided with a bypass valve and is opened and closed by a bypass valve rod 7, two ends of the main through pipe on the three, The inoculation bottle 5 is connected, a bypass pipe 8 of the three-way antibiotic stop valve 10 is connected with the steam pipe 6, and when the stop valve rod 9 is closed, the bypass pipe 8 can be communicated with the inoculation bottle 5.
When initial sterilization is carried out, a bypass valve rod 7 and a stop valve rod 9 are opened to enable a bypass pipe 8, an inoculation bottle 5 and a seed tank 11 to be in a three-way state, high-temperature steam is introduced into the bypass pipe 8 and filled into the inoculation bottle 5 and the seed tank 11 to further achieve sterilization, the sterilized steam is discharged from an exhaust hole 3 of an inoculation hood 2 on the inoculation bottle 5 to ensure the completeness of sterilization, inoculation is carried out after the sterilization is finished, the stop valve rod 9 and the bypass valve rod 7 are closed, a closed inoculation fire ring is placed on a fire tray during inoculation, liquid is poured into the inoculation bottle 5 under the protection of flame, the inoculation hood 2 is screwed, culture medium is simultaneously injected into the seed tank 11, the pressure in the seed tank 11 rises, the stop valve rod 9 is opened, the pressure in the seed tank 11 is reduced, the stop valve rod 9 is closed after the liquid flows into the seed tank 11, and the seed tank 11 is in a sealed pressure maintaining state, the seeding tank 11 is removed with the seed liquid and then is supplemented with new culture medium, meanwhile, the bypass valve rod 7 is opened and high-temperature steam is introduced into the inoculation bottle 5 to independently sterilize the inoculation bottle 5, the inoculation process is repeated after the sterilization is finished, because only the seeding bottle 5 is sterilized at high temperature independently during the subsequent sterilization, and the seeding tank 11 is in a sealed state after the seeding bottle 5 is sterilized and when the seed liquid is poured in, therefore, the processes of sterilizing and injecting the inoculation bottle 5 and the process of feeding and replenishing the liquid in the seeding tank 11 can be synchronously carried out, namely, after the seeding tank 11 discharges the last batch of inoculated culture medium and replenishes a new continuous sterile culture medium, the inoculation bottle 5 is sterilized and injected, therefore, next batch of inoculation operation can be directly carried out, continuous inoculation of the seeding tank 11 is realized, continuous production is further realized, meanwhile, the steam usage amount is reduced, and the energy consumption is greatly reduced.
In this embodiment, it is specific, the mouth of pipe at main siphunculus both ends is connected with dona tub 11, inoculation bottle 5 through flange 12 respectively, inoculation bottle 5 is made for stainless steel material, fixed mounting has stress application pole 1 on the inoculation hood 2, the periphery of inoculation bottle 5 just is located and is close to inoculation hood 2 department fixed mounting has tray 4.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (5)
1. The utility model provides a continuous inoculation device, includes inoculation hood, inoculation bottle, seed tank and inoculation valve, the open-top of inoculation bottle, inoculation hood threaded connection in the open-top of inoculation bottle, the last exhaust hole of having seted up of inoculation hood, the inoculation valve install the inoculation bottle with between the seed tank its characterized in that: the inoculation valve is a three-way antibiotic stop valve, two ends of a main through pipe on the three-way antibiotic stop valve are respectively connected with the seed tank and the inoculation bottle, a bypass pipe of the three-way antibiotic stop valve is connected with a steam pipe, and when the stop valve rod is closed, the bypass pipe can be communicated with the inoculation bottle.
2. The continuous inoculating device of claim 1, wherein: the pipe orifices at the two ends of the main through pipe are respectively connected with the seed tank and the inoculation bottle through connecting flanges.
3. The continuous inoculating device of claim 1, wherein: the inoculation bottle is made of stainless steel materials.
4. The continuous inoculating device of claim 1, wherein: and a stress application rod is fixedly arranged on the inoculation hood.
5. The continuous inoculating device of claim 1, wherein: the periphery of inoculation bottle just is located and is close to inoculation hood department fixed mounting has the tray fire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020583106.XU CN212199248U (en) | 2020-04-20 | 2020-04-20 | Continuous inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020583106.XU CN212199248U (en) | 2020-04-20 | 2020-04-20 | Continuous inoculation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212199248U true CN212199248U (en) | 2020-12-22 |
Family
ID=73834025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020583106.XU Active CN212199248U (en) | 2020-04-20 | 2020-04-20 | Continuous inoculation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212199248U (en) |
-
2020
- 2020-04-20 CN CN202020583106.XU patent/CN212199248U/en active Active
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