CN202322844U

CN202322844U - Strain transferring device

Info

Publication number: CN202322844U
Application number: CN 201120475777
Authority: CN
Inventors: 谢昌贤; 刘运添; 王福军; 谢雪峰; 王鹏飞
Original assignee: JINHE BIOTECHNOLOGY CO Ltd
Current assignee: JINHE BIOTECHNOLOGY CO Ltd
Priority date: 2011-11-25
Filing date: 2011-11-25
Publication date: 2012-07-11
Anticipated expiration: 2021-11-25

Abstract

The utility model relates to a strain transferring device, which comprises a transferring device and a fermentation device, wherein the transferring device comprises an inoculation bottle, a cork is arranged at the opening of the inoculation bottle, and a strain transferring tube is arranged on the cork; and the fermentation device comprises a fermentation bottle, a strain guide tube is arranged at the top of the fermentation bottle, a valve is arranged on the strain guide tube, and the end part of the strain guide tube is provided with an inoculation head which is used for detachably butting with the strain transferring tube in a sealing way. The strain transferring device is used for transferring and enlarging strains during the fermentation, is suitable for the inoculation operation of bacteria, actinomycetes, mould and fungus, an inoculation opening is not easy to block, and meanwhile microbial contamination is avoided.

Description

The culture transferring device

Technical field

The utility model relates to the fermentation unit field, particularly the culture transferring device in bacterial classification switching and the amplification culture process.

Technical background

At present, the general micropore inoculation method that adopts is inoculated in microbial fermentation enterprise, and seed liquor adopts pressure differential method to insert in seeding tank or the fermentor tank under the flame protection.This classification inoculation apparatus mainly is made up of aseptic eggplant-shape bottle or square vase, anchor clamps, sleeve pipe and syringe needle; Its principle is: eggplant-shape bottle or square vase are packed in the anchor clamps, install the inoculation syringe needle, add sleeve pipe at syringe needle; Close the clamp of anchor clamps, eggplant-shape bottle or square vase are fixed with anchor clamps.Before the inoculation,, it is 121 ℃ in temperature with gauze and kraft paper parcel; Sterilization in 40 minutes 2 times in sterilisable chamber, is untied the gauze and the kraft paper of classification inoculation apparatus during inoculation; Take off sleeve pipe; Syringe needle is inserted the miniature inoculation mouth of fermentor tank, through the pressure of adjustment fermentor tank, seed liquor is inoculated in seeding tank or the fermentor tank then.Mainly there is following shortcoming in this classification inoculation apparatus: (1) classification inoculation apparatus adopts syringe needle to do the inoculation outlet; Bore is minimum so can only be applicable to bacterium or actinomycetic inoculation; For mycelia thicker mould and fungi, or the thicker bacterial classification of fermented liquid, this kind device often causes stopping up syringe needle; (2) syringe needle does that syringe needle very easily causes the fracture and the into jar that carries disease germs in the inoculation outlet use, because of pollution microbes causes the fermentation failure; (3) classification inoculation apparatus adopts glass material, uses the time generation explosion easily of pressure differential method control fermentor tank pressure.

The utility model content

In view of this, the technical problem that the utility model solves is the bore that increases the inoculation outlet, can be applicable to the thicker mould of mycelia, fungi or the thicker bacterial classification of fermented liquid, avoids living contaminants simultaneously.

For solving the problems of the technologies described above, the utility model adopts following technical scheme:

The culture transferring device comprises transfer device and fermentation unit, and said transfer device comprises inoculation bottle, and said fermentation unit comprises fermentation flask, and said inoculation bottle bottleneck is provided with bottle stopper, and said bottle stopper is provided with the culture transferring pipe; The top of said fermentation flask is provided with grain conductor, and grain conductor is provided with valve, and the end of grain conductor is provided with the inoculation head that is used for the detachable sealed butt joint of culture transferring pipe.

Further, said inoculation head is provided with the joint cap that be sealed and matched detachable with it.

Further, said joint cap cooperates with the inoculation head thread.

Further, it is step-like that the outer wall of said inoculation head and culture transferring pipe matching part circularizes tapering.

Further, said inoculation bottle, bottle stopper and culture transferring pipe coupling place are provided with anchor clamps, and said anchor clamps are made up of two retaining plates and the set nut that is positioned at two retaining plate two ends.

Further, also be provided with pipe connecting between said bottle stopper and the culture transferring pipe, said pipe connecting is a stainless steel tube, and internal diameter is more than or equal to 1cm.

Further, said bottle stopper is a silica gel plug.

Further, said inoculation bottle is the stainless steel triangular flask, and volume is 4-5L.

Further, said pipe connecting extends to distance bottle end 0.5-2cm place along said inoculation bottle direction.

Further, said culture transferring pipe is a silicone tube.

The beneficial effect of the utility model is that the disclosed culture transferring device of the utility model all improves inoculation container and fermentor tank; Improve between back inoculation container and the fermentor tank and inoculate through culture transferring pipe and inoculation mouth, its bore strengthens, and is applicable to the inoculation operation of bacterium, actinomycetes, mould and fungi; Be difficult for stopping up the inoculation mouth; And use stainless steel cylinder to be inoculation bottle, and prevented when pressure differential method is inoculated, to be prone to sudden and violent fried shortcoming, use the disclosed culture transferring device of the utility model; Aseptic convenient, simple, be difficult for polluting.

Description of drawings

Fig. 1 is the utility model transfer device structure iron;

Fig. 2 is the utility model fermentation unit structure iron;

Fig. 3 is the utility model inoculation junction enlarged image;

Fig. 4 improves figure for the utility model transfer device.

Embodiment

Below will be with reference to accompanying drawing, the preferred embodiment of the utility model is carried out detailed description.Should be appreciated that preferred embodiment has been merely explanation the utility model, rather than in order to limit the protection domain of the utility model.

Accompanying drawing 1 is the utility model transfer device structure iron, and transfer device comprises inoculation bottle 1, and inoculation bottle 1 is the stainless steel triangular flask, and volume is 4L, and volume also can be greater than 4L, as is processed as 5L.Bottle mouth position at inoculation bottle 1 is fixedly connected with bottle stopper 2, and bottle stopper 2 is preferably silica gel plug, also can be other stopping property bottle stoppers; Be fixedly connected with pipe connecting 9 at the bottle stopper top, pipe connecting 9 is a stainless steel tube, and internal diameter is 1cm; Also can be greater than 1cm; Pipe connecting 9 communicates with inoculation bottle 1, and more convenient pipe connecting 9 extends to distance bottle end 0.5-2cm place along inoculation bottle 1 direction during for culture transferring, shown in accompanying drawing 4.Be fixedly connected with culture transferring pipe 4 on pipe connecting 9 tops; Culture transferring pipe 4 is a flexible pipe; Be preferably silicone tube, culture transferring is set to arc with culture transferring pipe 4 for ease; Bottleneck, pipe connecting 9 and culture transferring pipe 4 junctions at bottle stopper 2, inoculation bottle 1 are provided with anchor clamps 3, and anchor clamps 3 are fixed bottle stopper 2, inoculation bottle 1, pipe connecting 9 and culture transferring pipe 4.Anchor clamps 3 have two retaining plates, and two retaining plate two ends are provided with set nut, and are through retaining plate that bottle stopper 2, inoculation bottle 1, pipe connecting 9 and culture transferring pipe 4 is fixing, make fixing more firm again with set nut.

Accompanying drawing 2 is the utility model fermentation unit structure iron, and fermentation unit comprises fermentation flask 10, is provided with grain conductor 8 at fermentation flask 10 tops; Grain conductor 8 is a stainless steel tube, and internal diameter is 1cm, also can be greater than 1cm; Grain conductor 8 communicates with fermentation flask 10; Said grain conductor 8 is provided with valve 7, is provided with in the end of grain conductor 8 to be used for and the inoculation of culture transferring pipe 4 detachable sealed butt joint 6, and inoculation 6 is provided with the joint cap that be sealed and matched 5 detachable with it.Inoculation 6 a lower outside wall are provided with screw thread, and inoculation cap 5 inner side-walls are provided with and inoculate 6 respective threads, joint cap (5) and inoculation head (6) thread fit.

Accompanying drawing 3 is the utility model inoculation cap enlarged view, and it is step-like that the outer wall of inoculation 6 and culture transferring pipe 4 matching parts circularizes tapering, when culture transferring, culture transferring pipe 4 is enclosed within the inoculation 6, when using pressure differential method to shift, makes culture transferring pipe 4 combine more firm with inoculation mouth 6.

Before the use, transfer device and fermentation unit are wrapped with kraft paper, the port that need to connect the culture transferring pipe 4 of inoculation 6 wraps sterilization then separately.During culture transferring, the kraft paper of unpacking is opened inoculation cap 5; The culture transferring pipe 4 of transfer device is enclosed within the inoculation 6, tightens anchor clamps 3, open valve 7 and make fermentation flask 10 and inoculate 6 and communicate; Utilize differential pressure method, the seed liquor culture transferring in the inoculation bottle 1 is comprised fermentation flask 10 to fermentation unit.Inoculation finishes, and valve-off 7 is pulled up culture transferring pipe 4, covers inoculation cap 5 and tightens, and ferments.

Explanation is at last; Above embodiment is only unrestricted in order to this practical art scheme to be described; Although the preferred embodiment through with reference to the utility model is described the utility model; But those of ordinary skill in the art should be appreciated that and can make various changes to it in form with on the details, and the spirit and the scope of the utility model that does not depart from appended claims and limited.

Claims

1. culture transferring device; Comprise transfer device and fermentation unit, said transfer device comprises inoculation bottle (1), and said fermentation unit comprises fermentation flask (10); It is characterized in that: said inoculation bottle (1) bottleneck is provided with bottle stopper (2), and said bottle stopper (2) is provided with culture transferring pipe (4); The top of said fermentation flask (10) is provided with grain conductor (8), and grain conductor (8) is provided with valve (7), and the end of grain conductor (8) is provided with the inoculation head (6) that is used for the detachable sealed butt joint of culture transferring pipe (4).

2. according to the said culture transferring device of claim 1, it is characterized in that: said inoculation head (6) is provided with the joint cap that be sealed and matched detachable with it (5).

3. according to the said culture transferring device of claim 2, it is characterized in that: said joint cap (5) and inoculation head (6) thread fit.

4. according to the said culture transferring device of claim 3, it is characterized in that: it is step-like that the outer wall of said inoculation head (6) and culture transferring pipe (4) matching part circularizes tapering.

5. according to the said culture transferring device of claim 1; It is characterized in that: said inoculation bottle (1), bottle stopper (2) and culture transferring pipe (4) junction are provided with anchor clamps (3), and said anchor clamps (3) are made up of two retaining plates and the set nut that is positioned at two retaining plate two ends.

6. according to the said culture transferring device of claim 5, it is characterized in that: also be provided with pipe connecting (9) between said bottle stopper (2) and the culture transferring pipe (4), said pipe connecting is a stainless steel tube, and internal diameter is more than or equal to 1cm.

7. according to the said culture transferring device of claim 6, it is characterized in that: said bottle stopper (2) is a silica gel plug.

8. according to the said culture transferring device of claim 7, it is characterized in that: said inoculation bottle (1) is the stainless steel triangular flask, and volume is 4-5L.

9. said according to Claim 8 culture transferring device is characterized in that: said pipe connecting (8) extends to distance bottle end 0.5-2cm place along said inoculation bottle (1) direction.

10. according to the said culture transferring device of claim 9, it is characterized in that: said culture transferring pipe (4) is a silicone tube.