CN209113873U - A kind of anaerobism closing classification inoculation apparatus - Google Patents
A kind of anaerobism closing classification inoculation apparatus Download PDFInfo
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- CN209113873U CN209113873U CN201821678918.1U CN201821678918U CN209113873U CN 209113873 U CN209113873 U CN 209113873U CN 201821678918 U CN201821678918 U CN 201821678918U CN 209113873 U CN209113873 U CN 209113873U
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- 238000011081 inoculation Methods 0.000 title claims abstract description 92
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 239000007789 gas Substances 0.000 claims description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims 2
- 239000006071 cream Substances 0.000 claims 1
- 230000008676 import Effects 0.000 claims 1
- 239000004816 latex Substances 0.000 abstract description 7
- 229920000126 latex Polymers 0.000 abstract description 7
- 239000000243 solution Substances 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000013013 elastic material Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000605716 Desulfovibrio Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本实用新型公开了一种厌氧封闭接种装置，包括接种罐、连接口、接种针、快装接头和气瓶，接种罐的顶部设置有进料口和排气口，排气口设置在进料口的一侧，进料口的顶部设置有密封盖，接种罐的一侧设置有蒸汽出口，接种罐的另一侧设置有连接口、压力表和蒸汽进口，连接口、压力表和蒸汽进口从上到下依次设置在接种罐的外壁上，连接口与第一软管固定连接，第一软管的底端设置有接种针，接种针设置有四个，接种针包括针管、乳胶管和针头，乳胶管的一端与针管连接，乳胶管的另一端与针头连接，接种罐一侧底端通过导管与气瓶连接。该接种装置操作简单，方便快捷，延长了装置的使用寿命，能够保证接种装置环境的安全性，不易污染，提高了接种效率。
The utility model discloses an anaerobic closed inoculation device, which comprises an inoculation tank, a connection port, an inoculation needle, a quick-fit joint and a gas cylinder. The top of the inoculation tank is provided with a feeding port and an exhaust port, and the exhaust port is arranged at the feeding port. One side of the port, the top of the feed port is provided with a sealing cover, one side of the inoculation tank is provided with a steam outlet, the other side of the inoculation tank is provided with a connection port, a pressure gauge and a steam inlet, and the connection port, pressure gauge and steam inlet are provided. They are arranged on the outer wall of the inoculation tank in order from top to bottom, and the connection port is fixedly connected with the first hose. The bottom end of the first hose is provided with inoculation needles, and there are four inoculation needles. The inoculation needles include a needle tube, a latex tube and a Needle, one end of the latex tube is connected to the needle tube, the other end of the latex tube is connected to the needle, and the bottom end of one side of the inoculation tank is connected to the gas cylinder through a conduit. The inoculation device is simple to operate, convenient and quick, prolongs the service life of the device, ensures the safety of the inoculation device environment, is not easy to be polluted, and improves the inoculation efficiency.
Description
技术领域technical field
本实用新型涉及接种装置，特别涉及一种厌氧封闭接种装置，属于微生物厌氧发酵接种设备技术领域。The utility model relates to an inoculation device, in particular to an anaerobic closed inoculation device, which belongs to the technical field of microbial anaerobic fermentation inoculation equipment.
背景技术Background technique
厌氧，指一个生物体或细胞能在分子氧缺乏或不存在下生长，不需要游离氧能生长的一种微生物如脱硫弧菌，可以利用厌氧产气袋法进行厌氧培养，规格2.5L的产气袋只能将2.5L容积内的氧气完全吸收，转化成二氧化碳，同理，3.5L的产气袋能吸收3.5L容积的氧气。微需要产气袋和二氧化碳产气袋亦是如此，为达到相应的氧气浓度和二氧化碳浓度，不仅容积要固定，放置的培养物数量也基本要装满，在出厂前都根据计算设定好了的，所以，培养也需要在设定的条件下进行。Anaerobic, refers to a microorganism or cell that can grow in the absence or absence of molecular oxygen and does not require free oxygen to grow, such as Desulfovibrio, which can be cultured anaerobic by the anaerobic air bag method, specification 2.5 The L air bag can only completely absorb the oxygen in the 2.5L volume and convert it into carbon dioxide. Similarly, the 3.5L air bag can absorb 3.5L of oxygen. The same is true for micro-need gas production bags and carbon dioxide gas production bags. In order to achieve the corresponding oxygen concentration and carbon dioxide concentration, not only the volume must be fixed, but the number of cultures placed must also be basically filled, which are set according to calculations before leaving the factory. Therefore, the culture also needs to be carried out under the set conditions.
目前，厌氧发酵接种设备常用的接种瓶是由玻璃制作的克氏瓶，瓶内没有灭菌装置，接种时，在发酵罐接种口套上火圈灭菌，然后，工作人员赤手拿着克氏瓶往接种口内倾倒菌种液。由于罐内是常压，在接种时外界细菌很容易进入到罐内，因而影响了厌氧接种的环境，同时也对工作人员的实际技能要求非常高，降低了工作效率，并且易造成玻璃瓶破损。At present, the commonly used inoculation bottle for anaerobic fermentation inoculation equipment is a Kjeldahl bottle made of glass. There is no sterilization device in the bottle. During inoculation, a fire ring is placed on the inoculation mouth of the fermenter to sterilize. Then, the staff holds the gram with bare hands. Pour the inoculum solution into the inoculation port. Due to the normal pressure in the tank, it is easy for external bacteria to enter the tank during inoculation, which affects the environment of anaerobic inoculation. At the same time, it also requires very high practical skills of the staff, which reduces work efficiency and easily causes glass bottles. damaged.
发明内容SUMMARY OF THE INVENTION
本实用新型要解决的技术问题是克服现有接种装置效率低、易损坏的缺陷，提供一种厌氧封闭接种装置。The technical problem to be solved by the utility model is to overcome the defects of low efficiency and easy damage of the existing inoculation device, and to provide an anaerobic closed inoculation device.
为了解决上述技术问题，本实用新型提供了如下的技术方案：In order to solve the above-mentioned technical problems, the utility model provides the following technical solutions:
本实用新型提供一种厌氧封闭接种装置，包括接种罐、连接口、接种针、快装接头和气瓶，所述接种罐的顶部设置有进料口和排气口，所述排气口设置在进料口的一侧，所述进料口的顶部设置有密封盖，所述接种罐的一侧设置有蒸汽出口，所述接种罐的另一侧设置有连接口、压力表和蒸汽进口，所述连接口、压力表和蒸汽进口从上到下依次设置在接种罐的外壁上，所述连接口与第一软管固定连接，所述第一软管的底端设置有接种针，所述接种针设置有四个，所述接种针包括针管、乳胶管和针头，所述乳胶管的一端与针管连接，所述乳胶管的另一端与针头连接，所述接种罐一侧底端通过导管与气瓶连接，所述接种罐的底端连接有第二软管，所述第二软管的底端设置有快装接头，所述第二软管与快装接头固定连接，所述蒸汽出口、蒸汽进口、连接口、导管和第二软管上均设置有控制阀门。The utility model provides an anaerobic closed inoculation device, comprising an inoculation tank, a connection port, an inoculation needle, a quick-fit joint and a gas cylinder. The top of the inoculation tank is provided with a feeding port and an exhaust port, and the exhaust port is provided with On one side of the feeding port, the top of the feeding port is provided with a sealing cover, one side of the inoculation tank is provided with a steam outlet, and the other side of the inoculation tank is provided with a connection port, a pressure gauge and a steam inlet , the connection port, the pressure gauge and the steam inlet are sequentially arranged on the outer wall of the inoculation tank from top to bottom, the connection port is fixedly connected with the first hose, and the bottom end of the first hose is provided with an inoculation needle, The inoculation needle is provided with four, the inoculation needle includes a needle tube, a latex tube and a needle, one end of the latex tube is connected to the needle tube, the other end of the latex tube is connected to the needle, and the bottom end of one side of the inoculation tank is It is connected with the gas cylinder through a conduit, the bottom end of the inoculation tank is connected with a second hose, the bottom end of the second hose is provided with a quick-fit joint, and the second hose is fixedly connected with the quick-fit joint, so The steam outlet, the steam inlet, the connection port, the conduit and the second hose are all provided with control valves.
作为本实用新型的一种优选的技术方案，四个所述接种针呈两排等间距设置。As a preferred technical solution of the present invention, the four inoculation needles are arranged in two rows at equal intervals.
作为本实用新型的一种优选的技术方案，所述针管上还设置有控制阀门。As a preferred technical solution of the present invention, the needle tube is also provided with a control valve.
作为本实用新型的一种优选的技术方案，所述气瓶内充有氮气。As a preferred technical solution of the present invention, the gas cylinder is filled with nitrogen.
作为本实用新型的一种优选的技术方案，所述密封盖为弹性材料制成。As a preferred technical solution of the present invention, the sealing cover is made of elastic material.
作为本实用新型的一种优选的技术方案，所述针头的材料为不锈钢。As a preferred technical solution of the present invention, the material of the needle head is stainless steel.
作为本实用新型的一种优选的技术方案，所述快装接头接口与需接种的发酵罐顶部设有的接种口相配套。As a preferred technical solution of the present invention, the quick-fit joint interface is matched with the inoculation port provided on the top of the fermenter to be inoculated.
本实用新型所达到的有益效果是：该接种装置操作简单，方便快捷，延长了装置的使用寿命，能够保证接种装置环境的安全性，不易污染，提高了接种效率。The beneficial effects achieved by the utility model are: the inoculation device is easy to operate, convenient and quick, prolongs the service life of the device, can ensure the safety of the inoculation device environment, is not easy to be polluted, and improves the inoculation efficiency.
附图说明Description of drawings
附图用来提供对本实用新型的进一步理解，并且构成说明书的一部分，与本实用新型的实施例一起用于解释本实用新型，并不构成对本实用新型的限制。在附图中：The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and are used to explain the present invention together with the embodiments of the present invention, and do not constitute a limitation to the present invention. In the attached image:
图1是本实用新型的结构示意图；Fig. 1 is the structural representation of the present utility model;
图中：1、密封盖；2、进料口；3、连接口；4、第一软管；5、接种针；6、压力表；7、蒸汽进口；8、第二软管；9、快装接头；10、排气口；11、接种罐；12、蒸汽出口；13、导管；14、控制阀门；15、气瓶；16、针管；17、乳胶管；18、针头。In the figure: 1. Sealing cover; 2. Feeding port; 3. Connecting port; 4. First hose; 5. Inoculation needle; 6. Pressure gauge; 7. Steam inlet; 8. Second hose; 9. Quick connector; 10, exhaust port; 11, inoculation tank; 12, steam outlet; 13, catheter; 14, control valve; 15, gas cylinder; 16, needle tube; 17, latex tube; 18, needle.
具体实施方式Detailed ways
以下结合附图对本实用新型的优选实施例进行说明，应当理解，此处所描述的优选实施例仅用于说明和解释本实用新型，并不用于限定本实用新型。The preferred embodiments of the present utility model will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present utility model, but not to limit the present utility model.
实施例1：Example 1:
如图1所示，本实用新型提供一种厌氧封闭接种装置，包括接种罐11、连接口3、接种针5、快装接头9和气瓶15，接种罐11的顶部设置有进料口2和排气口10，排气口10设置在进料口2的一侧，进料口2的顶部设置有密封盖1，接种罐11的一侧设置有蒸汽出口12，接种罐11的另一侧设置有连接口3、压力表6和蒸汽进口7，连接口3、压力表6和蒸汽进口7从上到下依次设置在接种罐11的外壁上，连接口3与第一软管4固定连接，第一软管4的底端设置有接种针5，接种针5设置有四个，接种针5包括针管16、乳胶管17和针头18，乳胶管17的一端与针管16连接，乳胶管17的另一端与针头18连接，接种罐11一侧底端通过导管13与气瓶15连接，接种罐11的底端连接有第二软管8，第二软管8的底端设置有快装接头9，第二软管8与快装接头9固定连接，蒸汽出口12、蒸汽进口7、连接口3、导管13和第二软管8上均设置有控制阀门14。As shown in FIG. 1, the utility model provides an anaerobic closed inoculation device, including an inoculation tank 11, a connection port 3, an inoculation needle 5, a quick-fit joint 9 and a gas cylinder 15, and the top of the inoculation tank 11 is provided with a feeding port 2 And the exhaust port 10, the exhaust port 10 is provided on one side of the feed port 2, the top of the feed port 2 is provided with a sealing cover 1, one side of the inoculation tank 11 is provided with a steam outlet 12, and the other side of the inoculation tank 11 is provided with a steam outlet 12. The side is provided with a connection port 3, a pressure gauge 6 and a steam inlet 7. The connection port 3, the pressure gauge 6 and the steam inlet 7 are sequentially arranged on the outer wall of the inoculation tank 11 from top to bottom, and the connection port 3 is fixed with the first hose 4. Connection, the bottom end of the first hose 4 is provided with an inoculation needle 5, and four inoculation needles 5 are provided. The other end of 17 is connected to the needle 18, the bottom end of one side of the inoculation tank 11 is connected to the gas cylinder 15 through the conduit 13, the bottom end of the inoculation tank 11 is connected with a second hose 8, and the bottom end of the second hose 8 is provided with a fast The assembly joint 9 is fixedly connected with the second hose 8 and the quick assembly joint 9 . The steam outlet 12 , the steam inlet 7 , the connection port 3 , the conduit 13 and the second hose 8 are all provided with a control valve 14 .
四个接种针5呈两排等间距设置，能够插入接种罐11的进料口2中，方便接种针的随意取换，方便接种；针管16上还设置有控制阀门14，能够控制注入接种罐11中物料的量，方便人工控制接种数量；气瓶15内充有氮气，用于排除氧气，制造厌氧环境；密封盖1为弹性材料制成，密封效果好；针头18的材料为不锈钢，耐腐蚀性能好，不易损坏，方便进行消毒处理；快装接头9接口与需接种的发酵罐顶部设有的接种口相配套，便于快速接种。The four inoculation needles 5 are arranged in two rows at equal intervals, and can be inserted into the feeding port 2 of the inoculation tank 11, which is convenient for the random replacement of the inoculation needles and facilitates inoculation; the needle tube 16 is also provided with a control valve 14, which can control the injection into the inoculation tank. The amount of material in 11 is convenient to manually control the amount of inoculation; the gas cylinder 15 is filled with nitrogen, which is used to remove oxygen and create an anaerobic environment; the sealing cover 1 is made of elastic material, and the sealing effect is good; the material of the needle 18 is stainless steel, It has good corrosion resistance, is not easy to be damaged, and is convenient for disinfection; the 9 interface of the quick-release joint is matched with the inoculation port on the top of the fermenter to be inoculated, which is convenient for rapid inoculation.
具体的，在使用时，先通过蒸汽进口7对接种罐11罐内通蒸汽进行消毒，然后打开连接气瓶15与接种罐11的导管13上的控制阀门14，使气瓶15内的氮气进入到接种罐11内，根据压力表6的数值调节接种罐11内的压力为微正压，然后关闭导管13上的控制阀门14，再将第一软管4的底端设置的接种针5插入进料口2中，在无菌环境中，向接种罐11内注入菌种液，注入完毕后，用密封盖1密封好，接种时，将快装接头9与发酵罐接种口连接好，打开第二软管8上的控制阀门14，使接种罐11与发酵罐压力平衡，然后关闭发酵罐进气阀，打开发酵罐放气阀泄压，菌液在罐压的作用下快速流入发酵罐，待菌种压完后，拆下快装接头9即可。Specifically, when in use, first pass steam in the inoculation tank 11 through the steam inlet 7 to sterilize the inoculation tank 11, and then open the control valve 14 on the conduit 13 connecting the gas cylinder 15 and the inoculation tank 11, so that the nitrogen in the gas cylinder 15 enters the Go into the inoculation tank 11, adjust the pressure in the inoculation tank 11 to a slightly positive pressure according to the value of the pressure gauge 6, then close the control valve 14 on the conduit 13, and then insert the inoculation needle 5 provided at the bottom of the first hose 4 into In the feeding port 2, in a sterile environment, inject the bacterial strain liquid into the inoculation tank 11. After the injection is completed, seal it with the sealing cover 1. When inoculating, connect the quick connector 9 to the inoculation port of the fermenter tank, open it. The control valve 14 on the second hose 8 balances the pressure between the inoculation tank 11 and the fermenter, then closes the inlet valve of the fermenter, opens the air release valve of the fermenter to release the pressure, and the bacterial liquid quickly flows into the fermenter under the action of the tank pressure. , after the strain is pressed, remove the quick connector 9.
本实用新型所达到的有益效果是：该接种装置操作简单，方便快捷，延长了装置的使用寿命，能够保证接种装置环境的安全性，不易污染，提高了接种效率。The beneficial effects achieved by the utility model are: the inoculation device is easy to operate, convenient and quick, prolongs the service life of the device, can ensure the safety of the inoculation device environment, is not easy to be polluted, and improves the inoculation efficiency.
最后应说明的是：以上所述仅为本实用新型的优选实施例而已，并不用于限制本实用新型，尽管参照前述实施例对本实用新型进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本实用新型的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本实用新型的保护范围之内。Finally, it should be noted that the above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. , it is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements to some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims (7)
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