CN203128570U - Denitrification authentication prefabricated pipe - Google Patents
Denitrification authentication prefabricated pipe Download PDFInfo
- Publication number
- CN203128570U CN203128570U CN 201320165680 CN201320165680U CN203128570U CN 203128570 U CN203128570 U CN 203128570U CN 201320165680 CN201320165680 CN 201320165680 CN 201320165680 U CN201320165680 U CN 201320165680U CN 203128570 U CN203128570 U CN 203128570U
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- pipe
- collection
- confinement
- denitrification
- support bar
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Abstract
The utility model discloses a denitrification authentication prefabricated pipe, and belongs to an anaerobic gas production germ authentication pipe. Pipe openings are respectively formed in the top and the bottom of an authentication pipe main body; the pipe opening at the top is connected with a gas collection pipe; the pipe opening at the bottom is connected with a sealing cover or a needle head connection cap; a supporting rod is inserted into the gas collection pipe; and the bottom of the supporting rod is movably connected with the bottom of the gas collection pipe. The denitrification authentication prefabricated pipe has the advantages that the upper end and the lower end of the authentication pipe are sealed by threads, so that under the condition that the authentication pipe is filled with a liquid culture medium, high-pressure moist heat sterilization is realized; scales are printed in a gas collection space; according to a piston theory, when a culture produces gas, a piston can be pushed; and the gas production performance can be judged according to the scales.
Description
Technical field:
The utility model belongs to a kind of anaerobic gas generation Bacteria Identification pipe, and particularly a kind of denitrification is identified prefabricated pipe.
Background technology:
In the microorganism identification culture experiment, often need test the gas deliverability of microorganism.The most frequently used method is to adopt Du Shi pipe (another name moral Han Shi tubule) to carry out the aerogenesis test at present, the Du Shi pipe of filling substratum is stood upside down put into the test tube that substratum is housed, and has bubble and stay in the Du Shi pipe so that observe when gas produces.But this method can not be carried out detection by quantitative to gas production rate, and Du Shi manages the more not convenient situation of existence when using.
Summary of the invention:
The purpose of this utility model is to overcome above-mentioned defective, and a kind of anaerobic gas generation Bacteria Identification pipe is provided, and thread seal is all adopted at the two ends up and down of assessor main body, is implemented in high pressure moist heat sterilization under the situation that liquid nutrient medium is housed; The collection and confinement of gases pipe is provided with scale, and support bar and gas collection tube adopt piston cylinder operator, piston can be promoted when culture has gas to produce, and can judge gas generation property according to scale.
The purpose of this utility model is achieved through the following technical solutions: a kind of denitrification is identified prefabricated pipe, it is characterized in that: comprise assessor main body, collection and confinement of gases pipe, support bar, sealing cover and inoculation syringe needle; The top of said assessor main body is provided with the top mouth of pipe, the top mouth of pipe and collection and confinement of gases pipe coupling, said support bar is in the top of collection and confinement of gases pipe is plugged in the collection and confinement of gases pipe, the bottom of the bottom of support bar and collection and confinement of gases pipe flexibly connects, the bottom of assessor main body is provided with the bottom mouth of pipe, on the mouth of pipe of bottom sealing cover is installed, the end of inoculation syringe needle is provided with attachment cap, is connected with the assessor bottom part body mouth of pipe.
The bottom of said support bar is provided with the sealing outside screw, and the sealing internal thread that arranges with collection and confinement of gases pipe bottom spins.
Said collection and confinement of gases pipe is provided with scale.
Sealing outside screw top, the bottom suit sealing rubber pad of said support bar.
The top of said support bar is provided with rotary handle.
Said assessor main body and collection and confinement of gases pipe are structure as a whole.
Said assessor main body, collection and confinement of gases pipe, support bar and inoculation syringe needle are PP or PE material.
Advantage of the present utility model and beneficial effect are:
1, in the assessor main body substratum is housed, thread seal is all adopted at the two ends up and down of assessor main body, can be implemented in high pressure moist heat sterilization under the situation that liquid nutrient medium is housed;
2, the collection and confinement of gases pipe is provided with scale, and support bar and gas collection tube adopt piston cylinder operator, piston can be promoted when culture has gas to produce, and can judge gas generation property according to scale;
3, both disposable use can be, also repeated use can be reclaimed.
Description of drawings:
Figure 1A is the assessor organigram.
Figure 1B uses synoptic diagram for assessor.
Fig. 1 C is assessor inoculation synoptic diagram.
Fig. 1 D is assessor aerogenesis synoptic diagram.
Wherein: 1-assessor main body, 2-collection and confinement of gases pipe, the sealing internal thread of 3-collection and confinement of gases pipe bottom, the sealing outside screw of 4-support bar bottom, 5-sealing rubber pad, 6-support bar, the 7-rotary handle, 8-is with the externally threaded mouth of pipe, the tapped bottom of 9-mouth of pipe sealing cover, 10-plastics inoculation syringe needle, the 11-substratum, the tapped attachment cap of 12-, the 13-top mouth of pipe, 14-scale.
Embodiment:
Embodiment: as shown in the figure, a kind of denitrification is identified prefabricated pipe, comprises assessor main body 1, collection and confinement of gases pipe 2, support bar 6, sealing cover 9 and inoculation syringe needle 10; The top of said assessor main body 1 is taper, conical top is provided with the top mouth of pipe 13, integral type connects collection and confinement of gases pipe 2 on the top mouth of pipe 13, the bottom of support bar 6 is in the top of collection and confinement of gases pipe 2 is plugged in collection and confinement of gases pipe 2, the bottom of support bar 6 is provided with sealing outside screw 4, the sealing internal thread 3 that arranges with collection and confinement of gases pipe bottom spins, the bottom of assessor main body 1 is provided with is with the externally threaded bottom mouth of pipe 8, the tapped ferrule lid 9 of spinning on the mouth of pipe 8 of bottom, the end of plastics inoculation syringe needle 10 is provided with tapped attachment cap 12, spins with the bottom mouth of pipe 8; Said collection and confinement of gases pipe 2 is provided with scale 14; The bottom sealing outside screw 4 top suit sealing rubber pads 5 of said support bar 6; The top of said support bar 6 is provided with rotary handle 7; Said assessor main body 1, collection and confinement of gases pipe 2, support bar 6 and inoculation syringe needle 10 are PP or PE material.
The utility model using method is as follows:
Step 1: the can of substratum
The sealing internal thread 3 of collection and confinement of gases pipe bottom and the sealing outside screw 4 of support bar bottom are remained on the state of outwarding winding, body is inverted, from the mouth of pipe 8, fill substratum, clockwise turn rotary handle 7, the sealing outside screw 4 of the sealing internal thread 3 of collection and confinement of gases pipe bottom and support bar bottom screws at this moment, can be with unnecessary substratum and gas emptying; With tapped ferrule lid 9 mouth of pipe 8 is screwed 115 ℃ of sterilization 25-30min; The cooling of sterilization back, this moment, assessor was finished product, shown in (Figure 1B);
Step 2: microbial inoculant
Under aseptic condition, assessor sealing cover 9 is outwarded winding, plastics are inoculated syringe needle 10 top attachment caps 12 to be screwed onto on the mouth of pipe 8, inoculation syringe needle 10 needle points immerse in the bacterial liquid culture, counterclockwise turn rotary handle 7, this moment, the sealing outside screw 4 of support bar bottom upwards unscrewed, when the sealing outside screw 4 of the sealing internal thread 3 bottom the collection and confinement of gases pipe and support bar bottom is outwarded winding fully, bacterial classification has been seeded in the substratum of assessor, at this moment, sealing rubber pad 5 lower edges are in collection and confinement of gases pipe 2 scales 0 ml position, shown in (Fig. 1 C); After inoculation finishes, plastics are inoculated syringe needle 10 pull up from assessor main body 1, with the mouth of pipe 8 screwing hermetics, leave standstill cultivation with sealing cover 9.
Step 3: the result observes
Assessor is after cultivating for some time, and piston apparatus can be come by jack-up microorganism if there is gas to produce then, shown in (Fig. 1 D).Be as the criterion with sealing rubber pad 5 lower edges and read scale, this scale is gas production rate.
Claims (7)
1. a denitrification is identified prefabricated pipe, it is characterized in that: comprise assessor main body, collection and confinement of gases pipe, support bar, sealing cover and inoculation syringe needle; The top of said assessor main body is provided with the top mouth of pipe, the top mouth of pipe and collection and confinement of gases pipe coupling, said support bar is in the top of collection and confinement of gases pipe is plugged in the collection and confinement of gases pipe, the bottom of the bottom of support bar and collection and confinement of gases pipe flexibly connects, the bottom of assessor main body is provided with the bottom mouth of pipe, on the mouth of pipe of bottom sealing cover is installed, the end of inoculation syringe needle is provided with attachment cap, is connected with the assessor bottom part body mouth of pipe.
2. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, and it is characterized in that: the bottom of said support bar is provided with the sealing outside screw, and the sealing internal thread that arranges with collection and confinement of gases pipe bottom spins.
3. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, and it is characterized in that: said collection and confinement of gases pipe is provided with scale.
4. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, it is characterized in that: sealing outside screw top, the bottom suit sealing rubber pad of said support bar.
5. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, and it is characterized in that: the top of said support bar is provided with rotary handle.
6. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, and it is characterized in that: said assessor main body and collection and confinement of gases pipe are structure as a whole.
7. a kind of denitrification as claimed in claim 1 is identified prefabricated pipe, and it is characterized in that: said assessor main body, collection and confinement of gases pipe, support bar and inoculation syringe needle are PP or PE material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320165680 CN203128570U (en) | 2013-04-03 | 2013-04-03 | Denitrification authentication prefabricated pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320165680 CN203128570U (en) | 2013-04-03 | 2013-04-03 | Denitrification authentication prefabricated pipe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203128570U true CN203128570U (en) | 2013-08-14 |
Family
ID=48936566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320165680 Expired - Fee Related CN203128570U (en) | 2013-04-03 | 2013-04-03 | Denitrification authentication prefabricated pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203128570U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540244A (en) * | 2018-07-03 | 2019-03-29 | 蜂巢能源科技有限公司 | Battery check device, battery detection equipment and battery detecting structure |
-
2013
- 2013-04-03 CN CN 201320165680 patent/CN203128570U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540244A (en) * | 2018-07-03 | 2019-03-29 | 蜂巢能源科技有限公司 | Battery check device, battery detection equipment and battery detecting structure |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130814 Termination date: 20190403 |
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| CF01 | Termination of patent right due to non-payment of annual fee |