CN204860463U - Can be used to add in succession tissue culture bottle of nutrient solution - Google Patents

Can be used to add in succession tissue culture bottle of nutrient solution Download PDF

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Publication number
CN204860463U
CN204860463U CN201520603924.0U CN201520603924U CN204860463U CN 204860463 U CN204860463 U CN 204860463U CN 201520603924 U CN201520603924 U CN 201520603924U CN 204860463 U CN204860463 U CN 204860463U
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China
Prior art keywords
bottle
tissue culture
nutrient solution
filling opening
blow vent
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CN201520603924.0U
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Chinese (zh)
Inventor
胡利伟
刘芳
牟文君
薛超群
王广山
王信民
郭建华
宋纪真
王爱国
戴华鑫
郭园园
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Zhengzhou Tobacco Research Institute of CNTC
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Zhengzhou Tobacco Research Institute of CNTC
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Priority to CN201520603924.0U priority Critical patent/CN204860463U/en
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Abstract

The utility model provides a can be used to add in succession tissue culture bottle of nutrient solution, includes the bottle and through the screw thread bottle lid together that kinks soon, its characterized in that: be provided with blow vent and filling opening on the bottle lid, and be provided with 0.25 mu m's filter membrane on the filling opening. The filling opening is a mouth of pipe that exceeds the bottle lid surface, internal diameter 0.4cm. Blow vent and the surperficial parallel and level of bottle lid, and be provided with the ventilated membrane on the blow vent. The utility model provides a can be high -efficient the novel tissue culture bottle of interpolation nutrient solution, this blake bottle quality is light, simple structure, easily operation, need not to open the bottle lid when adding the culture solution, the syringe that can adopt 10mL or 20mL leads to filtration membrane and add the nutrient solution in succession in the tissue culture bottle, has effectively reduced the amount of labour of extensive tissue culture in -process and the pollution rate among the tissue culture.

Description

A kind ofly can be used for the tissue culture flasks of adding nutrient solution continuously
Technical field
The utility model belongs to technical field of biological culture, is specifically related to a kind of tissue culture flasks can adding nutrient solution continuously.
Background technology
Plant Tissue Breeding is normally carried out in blake bottle, and along with the development of modern biotechnology and the extensively universal of tissue culture technique, tissue culture flasks obtains a large amount of application.Common tissue culture flasks is made up of bottle and plastic bottle closure, bottle cap many employings knob, and knob bottle cap is easy to operate, can reuse.But in long-term tissue culture procedures when nutrient solution is deficient, often need follow-up interpolation nutrient solution, if open knob bottle cap one by one to add nutrient solution, the time of meeting at substantial and the amount of labour.
Utility model content
The purpose of this utility model is just in order to overcome the deficiencies in the prior art, improve the operating efficiency, the reduction labour intensity that add culture fluid, and a kind of novel tissue blake bottle that efficiently can add nutrient solution is provided, this blake bottle light quality, structure be simple, be easy to operation, without the need to opening bottle cap during interpolation culture fluid, the amount of labour in extensive tissue culture procedures and the pollution rate in tissue cultures can be reduced.
The purpose of this utility model is achieved through the following technical solutions: a kind ofly can be used for the tissue culture flasks of adding nutrient solution continuously, the bottle cap (Merlon (PC) or polypropylene material (PP) can be adopted) comprising bottle and combined by screwed, wherein: on bottle cap, be provided with blow vent and filling opening, and on filling opening, be provided with the filter membrane of 0.25 μm.
Described filling opening is the mouth of pipe exceeding bottle cap surface, internal diameter 0.4cm, and the syringe by 10mL or 20mL adds nutrient solution by filter membrane continuously in tissue culture flasks.
Described blow vent is concordant with bottle cap surface, and blow vent is provided with ventilated membrane, and therefore liquid feeding process does not need to carry out in gnotobasis.
Feature of the present utility model is: (1), without the need to opening the bottle cap of tissue culture flasks, is directly carried out liquid feeding by the filling opening of the filter membrane with 0.25 μm, reduced the amount of labour in extensive tissue cultures.(2) blow vent can filtered air, can prevent miscellaneous bacteria from entering in tissue culture flasks and pollute, can meet again institute's culture material to the needs of air.Therefore, the novel tissue blake bottle that the utility model provides can be widely used in universities and colleges, scientific research and enterprises and institutions.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model tissue culture flasks preferred embodiment.
In figure: 1-filling opening, 2-filter membrane, 3-blow vent, 4-ventilated membrane, 5-bottle cap, 6-bottle.
Embodiment
The utility model is described further below in conjunction with accompanying drawing:
As shown in Figure 1: a kind ofly can be used for the tissue culture flasks of adding nutrient solution continuously, the plastic bottle closure 5 comprising glass body 6 and combined by screwed, wherein: on bottle cap 5, be provided with blow vent 3 and filling opening 1, and on filling opening 1, be provided with the filter membrane 2 of 0.25 μm.
Described filling opening 1 is the mouth of pipe exceeding bottle cap surface, internal diameter 0.4cm, and the syringe by 10mL or 20mL adds nutrient solution by filter membrane continuously in tissue culture flasks.
Described blow vent 3 is concordant with bottle cap surface, and blow vent 3 is provided with ventilated membrane 4, and therefore liquid feeding process does not need to carry out in gnotobasis.
When the utility model uses in Plant Tissue Breeding laboratory, cultivation bottle and plastic bottle closure newspaper are wrapped laggard horizontal high voltage sterilizing.Then on aseptic superclean bench, medium is poured into and cultivate in bottle, after putting into the plant tissue needing to cultivate, plastic bottle closure is loaded onto, be put on corresponding culturing rack and cultivate.After a period of time, along with the nutrient component consumption in culture fluid, the needs of bottle implants tissue growth can not be met, at this moment by the filling opening on bottle, the syringe of 10mL or 20mL can be adopted directly between tissue cultures, in tissue culture flasks, to add nutrient solution by filter membrane continuously, centre does not need open bottle cap and take out plant tissue, greatly reduces the workload in tissue culture procedures and pollution rate.

Claims (3)

1. can be used for a tissue culture flasks of adding nutrient solution continuously, comprise bottle and by the bottle cap that screwed combines, it is characterized in that: on bottle cap, being provided with blow vent and filling opening, and on filling opening, being provided with the filter membrane of 0.25 μm.
2. according to claim 1ly can be used for the tissue culture flasks of adding nutrient solution continuously, it is characterized in that: described filling opening is the mouth of pipe exceeding bottle cap surface, internal diameter 0.4cm.
3. according to claim 1ly can be used for the tissue culture flasks of adding nutrient solution continuously, it is characterized in that: described blow vent is concordant with bottle cap surface, and blow vent is provided with ventilated membrane.
CN201520603924.0U 2015-08-12 2015-08-12 Can be used to add in succession tissue culture bottle of nutrient solution Active CN204860463U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520603924.0U CN204860463U (en) 2015-08-12 2015-08-12 Can be used to add in succession tissue culture bottle of nutrient solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520603924.0U CN204860463U (en) 2015-08-12 2015-08-12 Can be used to add in succession tissue culture bottle of nutrient solution

Publications (1)

Publication Number Publication Date
CN204860463U true CN204860463U (en) 2015-12-16

Family

ID=54804894

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201520603924.0U Active CN204860463U (en) 2015-08-12 2015-08-12 Can be used to add in succession tissue culture bottle of nutrient solution

Country Status (1)

Country Link
CN (1) CN204860463U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105670930A (en) * 2016-03-25 2016-06-15 刘洪� Controllable-pressure bioreactor for bio-printed cartilage

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105670930A (en) * 2016-03-25 2016-06-15 刘洪� Controllable-pressure bioreactor for bio-printed cartilage

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