CN209368236U - A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium - Google Patents
A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium Download PDFInfo
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- CN209368236U CN209368236U CN201822030212.0U CN201822030212U CN209368236U CN 209368236 U CN209368236 U CN 209368236U CN 201822030212 U CN201822030212 U CN 201822030212U CN 209368236 U CN209368236 U CN 209368236U
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- tube
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- culture medium
- silicone tube
- way valve
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Abstract
The utility model discloses a kind of quick subpackage apparatuses of anaerobism solid-liquid culture medium, including nitrogen gas tank and liquid bottle, escape pipe is installed at the top of the nitrogen gas tank, and escape pipe is connected with three-way valve far from one end of nitrogen gas tank, one output end of the three-way valve is equipped with the first silicone tube, and first silicone tube far from the side of three-way valve be connected with stainless steel syringe needle, another output end of the three-way valve is equipped with the second silicone tube.The quick subpackage apparatus of anaerobism solid-liquid culture medium is provided with liquid separation mechanism, and when by screw on the inside of rotation liquid separation mechanism, the ring body of liquid measure control-rod outer wall is moved up and down, control liquid separation amount is completed convenient for the mechanism, meanwhile so that the device quickly dispenses for anaerobism solid-liquid culture medium in, it is reasonable in design, it can ensure that culture medium anaerobic, and simplify packing step, can quantitative separating, reduce experimental error, and manufacturing cost is low, application easy to promote large-scale.
Description
Technical field
The utility model relates to biologic test equipment engineering device technique field, specially a kind of anaerobism solid-liquid culture medium is quick
Subpackage apparatus.
Background technique
The packing of microorganism solid-liquid culture medium is the basic content of Experiment on Microbiology operation, solid slope culture medium
Production is the important medium of strain short-term preservation, and fluid nutrient medium is progress bacterial strain expanding species, the important means of culture, currently,
Microbiological culture media mainly uses glass funnel or pipette to be dispensed into culture tube one by one, and the packing of anaerobic culture medium does not have
Special equipment, so that needing to complete by the quick subpackage apparatus of anaerobism solid-liquid culture medium to dispense work.
Anaerobic culture medium packing in the market uses common micro-organisms culture medium method for filling, then carries out deoxygenation behaviour again
Make, method is cumbersome, and time-consuming, and once need to prepare mass propgation base when, exist packing it is time-consuming and laborious, low efficiency lack
Point, for this purpose, it is proposed that a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium.
Utility model content
The purpose of this utility model is to provide a kind of quick subpackage apparatuses of anaerobism solid-liquid culture medium, to solve above-mentioned back
Then the anaerobic culture medium packing proposed in scape technology carries out deoxygenation operation using common micro-organisms culture medium method for filling again,
Method is cumbersome, and time-consuming, and when once needing to prepare mass propgation base, there is the shortcomings that packing is time-consuming and laborious, low efficiency
Problem.
To achieve the above object, the utility model provides the following technical solutions: a kind of anaerobism solid-liquid culture medium quickly divides
Assembling device, including nitrogen gas tank and liquid bottle, are equipped with escape pipe at the top of the nitrogen gas tank, and escape pipe far from nitrogen gas tank one
End is connected with three-way valve, and an output end of the three-way valve is equipped with the first silicone tube, and the first silicone tube is far from threeway
The side of valve is connected with stainless steel syringe needle, and another output end of the three-way valve is equipped with the second silicone tube, and the second silicon
It is provided with glass tube in the middle part of sebific duct, sealing mechanism, and the top side peace of sealing mechanism are provided on the outside of the glass tube
It is equipped with liquid separation mechanism, the liquid bottle is external in the outside of nitrogen gas tank, and liquid bottle is internally provided with the 4th silicone tube, described
Nitrogen gas tank, liquid bottle bottom end be mounted with work top, the side of the liquid separation mechanism is circumscribed with culture tube.
Preferably, the shape of first silicone tube matches with the shape of the second silicone tube, and the first silica gel pipe end
Stainless steel syringe needle length be 12mm.
It preferably, include rubber stopper on the inside of the sealing mechanism, and the top inner side of rubber stopper offers fluid hole,
It is circumscribed with nitrogen blowhole on the outside of the fluid hole, and nitrogen blowhole is by injection molding and rubber stopper being integrally formed structure,
The inside top of the rubber stopper offers venthole.
Preferably, the area of section of the fluid hole is equal with the area of section of nitrogen blowhole, and fluid hole and nitrogen
Blowhole is symmetrical about the central axes of rubber stopper.
It preferably, include tube body on the inside of the liquid separation mechanism, and the bottom end of tube body is fixedly connected with glass sharp nozzle tube,
The bottom outside of the glass sharp nozzle tube is connected with the 4th silicone tube, is connected with outlet tube below the outer wall of the tube body, and
It is provided with third silicone tube in the middle part of outlet tube, is provided with fixator above the outer wall of the tube body, and pass through on the outside of fixator
It is installed with liquid measure control-rod, is screwed with screw above the outer wall of the liquid measure control-rod, it is living that glass is provided at the top of the tube body
Plug.
Preferably, the central axes of the glass sharp nozzle tube and the central axes of tube body coincide, and between tube body and rubber stopper
Perpendicular shape distribution.
Compared with prior art, the utility model has the beneficial effects that the quick subpackage apparatus of anaerobism solid-liquid culture medium
When being provided with liquid separation mechanism, and passing through the screw on the inside of rotation liquid separation mechanism, the lantern ring of liquid measure control-rod outer wall move down
It is dynamic, control liquid separation amount is completed convenient for the mechanism, meanwhile, so that being tied during the device quickly dispense for anaerobism solid-liquid culture medium
Structure design rationally, and simplifies packing step, can quantitative separating, reduce experimental error, and manufacturing cost is low, easy to promote large-scale
Using, and the central axes of glass sharp nozzle tube and the central axes of tube body coincide, and pass through the glass point to coincide with tube body central axes
Mouth pipe is inserted into the effect of fluid hole, can carry out carrying operation to the tube body that its top is fixedly connected, and avoids generating on the inside of tube body
Air pressure change, influence liquid on the inside of liquid bottle goes out liquid work；
The area of section of fluid hole is equal with the area of section of nitrogen blowhole, can be to glass by the effect of fluid hole
Glass sharp nozzle tube disposed, fixed operation, and under the action of nitrogen blowhole, can be convenient for the second silicone tube to liquid bottle
Interior logical nitrogen gives culture medium deoxygenation, and entire packing process requires logical nitrogen, and oxygen is effectively prevent to enter；
The length of the stainless steel syringe needle of first silica gel pipe end is 12mm, can be by the by the effect of stainless steel syringe needle
One silicone tube draws into culture tube that (culture tube top tape taenidium, and have inner cover, outer spiral cover to match with it, packing terminates
Cover, can prevent oxygen from entering culture tube), and in the case where stainless steel syringe needle 5 is inserted into the inside bottom effect of culture tube 11, just
In being passed through nitrogen toward culture tube inside, then expeling operation can be carried out to the oxygen on the inside of culture tube, it can be ensured that culture medium anaerobic.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model liquid separation mechanism structure schematic diagram；
Fig. 3 is the utility model sealing mechanism structural schematic diagram.
In figure: 1, nitrogen gas tank；2, escape pipe；3, three-way valve；4, the first silicone tube；5, stainless steel syringe needle；6, the second silicon
Sebific duct；7, glass tube；8, liquid bottle；9, sealing mechanism；901, rubber stopper；902, fluid hole；903, venthole；904, nitrogen enters
Stomata；10, liquid separation mechanism；1001, tube body；1002, glass sharp nozzle tube；1003, outlet tube；1004, third silicone tube；1005,
Fixator；1006, liquid measure control-rod；1007, screw；1008, glass piston；11, culture tube；12, the 4th silicone tube；13, work
Make table top.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Please refer to Fig. 1-3, the utility model provides a kind of technical solution: a kind of anaerobism solid-liquid culture medium quickly dispenses dress
Set, including nitrogen gas tank 1, escape pipe 2, three-way valve 3, the first silicone tube 4, stainless steel syringe needle 5, the second silicone tube 6, glass tube 7,
Liquid bottle 8, sealing mechanism 9, rubber stopper 901, fluid hole 902, venthole 903, nitrogen blowhole 904, liquid separation mechanism 10, tube body
1001, glass sharp nozzle tube 1002, outlet tube 1003, third silicone tube 1004, fixator 1005, liquid measure control-rod 1006, screw
1007, glass piston 1008, culture tube 11, the 4th silicone tube 12 and work top 13, the top of nitrogen gas tank 1 are equipped with escape pipe
2, and escape pipe 2 is connected with three-way valve 3 far from one end of nitrogen gas tank 1, an output end of three-way valve 3 is equipped with the first silica gel
Pipe 4, and the first silicone tube 4 is connected with stainless steel syringe needle 5, the shape and second of the first silicone tube 4 far from the side of three-way valve 3
The shape of silicone tube 6 matches, and the length of the stainless steel syringe needle 5 of 4 end of the first silicone tube is 12mm, passes through stainless steel syringe needle
5 effect, the first silicone tube 4 can be drawn into culture tube 11 (11 top tape taenidium of culture tube, and have inner cover, outward turning
Gai Yuqi matches, and packing terminates to cover, and can prevent oxygen from entering culture tube 11), and culture is inserted into stainless steel syringe needle 5
Under the inside bottom effect of pipe 11, convenient for toward nitrogen is passed through on the inside of culture tube 11, then can to the oxygen on the inside of culture tube 11 into
Row drives away operation；
Another output end of three-way valve 3 is equipped with the second silicone tube 6, and the middle part of the second silicone tube 6 is provided with glass
Pipe 7, the outside of glass tube 7 is provided with sealing mechanism 9, and the top side of sealing mechanism 9 is mounted with liquid separation mechanism 10, sealer
The inside of structure 9 includes rubber stopper 901, and the top inner side of rubber stopper 901 offers fluid hole 902, the section of fluid hole 902
Area is equal with the area of section of nitrogen blowhole 904, and fluid hole 902 and nitrogen blowhole 904 are about in rubber stopper 901
Axisymmetrical can dispose glass sharp nozzle tube 1002, fixed operation by the effect of fluid hole 902, and in nitrogen
Under the action of blowhole 904, culture medium deoxygenation, and entire point can be given convenient for the second silicone tube 6 to nitrogen is led in liquid bottle 8
Dress process requires logical nitrogen, effectively prevent oxygen to enter, the outside of fluid hole 902 is circumscribed with nitrogen blowhole 904, and nitrogen
Blowhole 904 offers venthole by injection molding and 901 being integrally formed structure of rubber stopper, the inside top of rubber stopper 901
903；
The inside of liquid separation mechanism 10 includes tube body 1001, and the bottom end of tube body 1001 is fixedly connected with glass sharp nozzle tube
1002, the central axes of glass sharp nozzle tube 1002 and the central axes of tube body 1001 coincide, and between tube body 1001 and rubber stopper 901
Perpendicular shape distribution, the effect of fluid hole 902, energy are inserted by the glass sharp nozzle tube 1002 to coincide with 1001 central axes of tube body
It is enough that carrying operation is carried out to the tube body 1001 that its top is fixedly connected, avoid the air pressure generated on the inside of tube body 1001 from changing,
Influence 8 inside liquid of liquid bottle goes out liquid work, and the bottom outside of glass sharp nozzle tube 1002 is connected with the 4th silicone tube 12, manages
It is connected with outlet tube 1003 below the outer wall of body 1001, and the middle part of outlet tube 1003 is provided with third silicone tube 1004, tube body
It is provided with fixator 1005 above 1001 outer wall, and the outside of fixator 1005 is through having liquid measure control-rod 1006, liquid measure control
It is screwed with screw 1007 above the outer wall of bar 1006 processed, the top of tube body 1001 is provided with glass piston 1008；
Liquid bottle 8 is external in the outside of nitrogen gas tank 1, and liquid bottle 8 is internally provided with the 4th silicone tube 12, nitrogen gas tank 1,
The bottom end of liquid bottle 8 is mounted with work top 13, and the side of liquid separation mechanism 10 is circumscribed with culture tube 11.
Working principle: for this kind of quick subpackage apparatus of anaerobism solid-liquid culture medium first by matching to culture medium
Operation is set, the charging feedstock in liquid bottle 8 is needed, culture medium solid-liquid configuration work is carried out, and dissolve by heating, boils and tentatively remove
The oxygen in culture medium is removed, and during removing to oxygen, need to start the valve at three-way valve 3, so that nitrogen
The nitrogen of 1 inside of gas tank enters in liquid bottle 8 along the connector of the second silicone tube 6 and glass tube 7, by nitrogen to dress liquid
The inside of bottle 8 is filled operation, so that oxygen is discharged, completes deoxygenation work, then, quantitative separating work is carried out, by first
The stainless steel syringe needle 5 of 4 end of silicone tube accesses deoxygenation in culture tube 11, rotation screw 1007, so that above 1001 outer wall of tube body
Lantern ring slide up and down, convenient for accurately adjusting the volume of packing, and pull glass piston 1008, by the inside of liquid bottle 8
Culture medium is dispensed to the culture tube 11 connecting with outlet tube 1003, completes quickly packing work, meanwhile, in the process of packing
In, by the effect of venthole 903, it is able to maintain that the air pressure of 8 inside of liquid bottle.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium, including nitrogen gas tank (1) and liquid bottle (8), it is characterised in that: institute
It states and is equipped at the top of nitrogen gas tank (1) escape pipe (2), and escape pipe (2) is connected with three-way valve far from the one end of nitrogen gas tank (1)
(3), an output end of the three-way valve (3) is equipped with the first silicone tube (4), and the first silicone tube (4) is far from three-way valve
(3) side is connected with stainless steel syringe needle (5), and another output end of the three-way valve (3) is equipped with the second silicone tube (6),
And second is provided with glass tube (7) in the middle part of silicone tube (6), is provided with sealing mechanism (9) on the outside of the glass tube (7), and
The top side of sealing mechanism (9) is mounted with liquid separation mechanism (10), and the liquid bottle (8) is external in the outside of nitrogen gas tank (1), and
Liquid bottle (8) is internally provided with the 4th silicone tube (12), the nitrogen gas tank (1), liquid bottle (8) bottom end be mounted with work
The side of table top (13), the liquid separation mechanism (10) is circumscribed with culture tube (11).
2. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium according to claim 1, it is characterised in that: described first
The shape of silicone tube (4) matches with the shape of the second silicone tube (6), and the stainless steel syringe needle (5) of the first silicone tube (4) end
Length be 12mm.
3. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium according to claim 1, it is characterised in that: the sealing
It include rubber stopper (901) on the inside of mechanism (9), and the top inner side of rubber stopper (901) offers fluid hole (902), it is described
It is circumscribed on the outside of fluid hole (902) nitrogen blowhole (904), and nitrogen blowhole (904) passes through injection molding and rubber stopper (901)
The inside top of being integrally formed structure, the rubber stopper (901) offers venthole (903).
4. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium according to claim 3, it is characterised in that: the liquid out
The area of section in hole (902) is equal with the area of section of nitrogen blowhole (904), and fluid hole (902) and nitrogen blowhole
(904) central axes about rubber stopper (901) are symmetrical.
5. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium according to claim 1, it is characterised in that: the liquid separation
It include tube body (1001) on the inside of mechanism (10), and the bottom end of tube body (1001) is fixedly connected with glass sharp nozzle tube (1002),
The bottom outside of the glass sharp nozzle tube (1002) is connected with the 4th silicone tube (12), below the outer wall of the tube body (1001)
It is connected with outlet tube (1003), and is provided with third silicone tube (1004) in the middle part of outlet tube (1003), the tube body (1001)
Outer wall above be provided with fixator (1005), and the outside of fixator (1005) is through having liquid measure control-rod (1006), described
It is screwed with screw (1007) above the outer wall of liquid measure control-rod (1006), is provided with glass piston at the top of the tube body (1001)
(1008)。
6. a kind of quick subpackage apparatus of anaerobism solid-liquid culture medium according to claim 5, it is characterised in that: the glass
The central axes of sharp nozzle tube (1002) and the central axes of tube body (1001) coincide, and between tube body (1001) and rubber stopper (901)
Perpendicular shape distribution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822030212.0U CN209368236U (en) | 2018-12-05 | 2018-12-05 | A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822030212.0U CN209368236U (en) | 2018-12-05 | 2018-12-05 | A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium |
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| Publication Number | Publication Date |
|---|---|
| CN209368236U true CN209368236U (en) | 2019-09-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822030212.0U Expired - Fee Related CN209368236U (en) | 2018-12-05 | 2018-12-05 | A kind of quick subpackage apparatus of anaerobism solid-liquid culture medium |
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| CN (1) | CN209368236U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
-
2018
- 2018-12-05 CN CN201822030212.0U patent/CN209368236U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190910 Termination date: 20211205 |
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| CF01 | Termination of patent right due to non-payment of annual fee |