CN208586299U - A kind of bacteria culture bottle convenient for sampling - Google Patents
A kind of bacteria culture bottle convenient for sampling Download PDFInfo
- Publication number
- CN208586299U CN208586299U CN201820713973.3U CN201820713973U CN208586299U CN 208586299 U CN208586299 U CN 208586299U CN 201820713973 U CN201820713973 U CN 201820713973U CN 208586299 U CN208586299 U CN 208586299U
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- China
- Prior art keywords
- sealing cover
- culture bottle
- bottle body
- plate
- sealing
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Abstract
The utility model discloses a kind of bacteria culture bottles convenient for sampling, it cultivates and is equipped with demarcation plate in bottle body, culture bottle body interior is divided into cultivation region and isolated area by demarcation plate, culture bottle body upper part is connected with seal member, seal member includes sealing cover, set bucket is socketed in sealing cover lower part, it covers inside bucket and culture bottle body is threadedly coupled, sealing plate is equipped with inside sealing cover, sealing plate and sealing cover top constitute storage space, one end of hose sequentially passes through sealing cover, sealing plate and demarcation plate, the interlude of hose is connected with check valve, suction ball is connected to hose upper end.The utility model discloses a kind of bacteria culture bottles convenient for sampling, the utility model need not frequently open sealing cover and other openings during sampling, only need to act on suction ball pressure and its negative pressure alternating action generated, culture medium can be sampled, the introducing of check valve also makes solution not cause the pollution of culture medium because of suck-back, and practicability is stronger.
Description
Technical field
The utility model relates to biologic test equipment technology fields, specifically design a kind of bacteria culture bottle convenient for sampling.
Background technique
Bacteria Culture technology is an important technology of bioengineering field, and culture bottle is the basic container of Bacteria Culture
One of.In traditional cultural method, the growth conditions of bacterium are constantly monitored in fluid nutrient medium, or to engineering
When the situation of bacterium express express target protein is measured, need to sample in the slave culture bottle of timing during the cultivation process.It operates herein
In closed culture bottleneck is opened, sterilize and bottleneck and close again after sampling, operate relatively cumbersome, and be easy to cause two
Secondary pollution.
In the prior art, number of patent application CN201120389398, CN201120549621, CN201320591643 and
The patent of CN201720784088 is all to open sample tap in the side wall of culture bottle, avoids and opens troublesome operation brought by bottleneck
It may with pollution.Although the mode that side wall opens up sample tap avoids troublesome operation brought by opening bottleneck and pollution is possible,
But the operating procedure of sterilizing sample tap and the pollution sources risk of sample tap are equally faced with after sample tap sampling.Therefore, this
Do not tackle the major problem fundamentally the mode of kind culture bottle sidewall opening.
Utility model content
In order to overcome, sampling bring disinfecting action is cumbersome in liquid medium within incubation and be easy to cause secondary dirt
The problem of dye, the utility model discloses a kind of bacteria culture bottle convenient for sampling, the utility model during sampling not
Sealing cover and other openings must frequently be opened, it is only necessary to suction ball pressure and its negative pressure alternating action generated are acted on, it can
Culture medium is sampled, the introducing of check valve also makes solution not cause the pollution of culture medium because of suck-back, and practicability is stronger.
To achieve the goals above, the technical solution adopted in the utility model is:
A kind of bacteria culture bottle convenient for sampling, including culture bottle body, culture bottle body is interior to be equipped with demarcation plate, demarcation plate
Culture bottle body interior is divided into cultivation region and isolated area, culture bottle body upper part is connected with seal member, and seal member includes
Sealing cover, set bucket are socketed in sealing cover lower part, are threadedly coupled inside set bucket with culture bottle body, are equipped with and seal inside sealing cover
Plate, sealing plate and sealing cover top constitute storage space, and one end of hose sequentially passes through sealing cover, sealing plate and demarcation plate, soft
The interlude of pipe is connected with check valve, and suction ball is connected to hose upper end.
Preferably, the culture bottle inner body wall is equipped with fixed block, and demarcation plate is fixed by fixed block.
Any of the above-described scheme is preferably, and the suction ball is ball.
Any of the above-described scheme is preferably, and through-hole is equipped among the sealing cover, sealing plate and demarcation plate, and through-hole passes through
The sealing of soft seal piece.
Any of the above-described scheme is preferably, and the outer wall and sealing cover of the set bucket are threadedly coupled, and covers inner wall and the culture of bucket
Bottle body is threadedly coupled.
Any of the above-described scheme is preferably, and the sealing plate is horizontally disposed, and sealing plate outer ring is equipped with and sealing cover mutual
The external screw thread matched.
Any of the above-described scheme is preferably, and the sealing plate lower part is equipped with knob, the hollow setting of knob.
Any of the above-described scheme is preferably, and the quantity of the check valve is two, and two check valves can be separately fixed at
The through hole of sealing cover and demarcation plate.
The beneficial effects of the utility model are: being equipped with demarcation plate in culture bottle body, demarcation plate is by culture bottle body interior
It is divided into cultivation region and isolated area, culture bottle body upper part is connected with seal member, and seal member includes sealing cover, and set bucket is socketed in
Sealing cover lower part is threadedly coupled inside set bucket with culture bottle body, is equipped with sealing plate inside sealing cover, on sealing plate and sealing cover
Portion constitutes storage space, and one end of hose sequentially passes through sealing cover, sealing plate and demarcation plate, and the interlude and check valve of hose connect
It connects, suction ball is connected to hose upper end.The utility model discloses a kind of bacteria culture bottle convenient for sampling, which is being taken
Sealing cover and other openings need not be frequently opened during sample, it is only necessary to act on suction ball pressure and its negative pressure generated alternating
Effect, can be sampled culture medium, and the introducing of check valve also makes solution not cause the pollution of culture medium because of suck-back,
Practicability is stronger.
Detailed description of the invention
Fig. 1 is the overall structure diagram of a preferred embodiment of the utility model；
Fig. 2 is the non-working condition structural schematic diagram of Fig. 1；
Fig. 3 is a local state schematic diagram of Fig. 2；
Fig. 4 is another local state schematic diagram of Fig. 2.
1, bottle body, 2, demarcation plate, 3, cultivation region, 4, isolated area, 5, sealing cover, 6, set bucket, 7, sealing plate, 8, storage are cultivated
Hide space, 9, hose, 10, check valve, 11, suction ball, 12, fixed block, 13, through-hole, 14, diaphragm seal, 15, knob.
Specific embodiment
The structural principle of the utility model and working principle are described in further detail with reference to the accompanying drawing.
As Figure 1-Figure 4, a kind of bacteria culture bottle convenient for sampling, including culture bottle body 1, are cultivated in bottle body 1
Equipped with demarcation plate 2, demarcation plate 2, which will be cultivated inside bottle body 1, is divided into cultivation region 3 and isolated area 4, to reduce the chance of infection.
It cultivates 1 inner wall of bottle body and is equipped with fixed block 12, demarcation plate 2 is fixed by fixed block 12.
Culture 1 top of bottle body is connected with seal member, and seal member includes sealing cover 5, and set bucket 6 is socketed in sealing cover 5
Lower part, 6 inside of set bucket and culture bottle body 1 are threadedly coupled, and the outer wall and sealing cover 5 for covering bucket 6 are threadedly coupled.
Sealing plate 7 is equipped with inside sealing cover 5, sealing plate 7 and 5 top of sealing cover constitute storage space 8, and sealing plate 7 is horizontal
Setting, the external screw thread that 7 outer ring of sealing plate is equipped with and sealing cover 5 is mutually matched.7 lower part of sealing plate is equipped with knob 15, and knob 15 is empty
Heart setting.
One end of hose 9 sequentially passes through sealing cover 5, sealing plate 7, knob 15 and demarcation plate 2, the interlude and list of hose 9
It is connected to valve 10, the quantity of check valve 10 is two, and two check valves 10 can be separately fixed at sealing plate 7 and demarcation plate 2
At through-hole 13.Suction ball 11 is connected to 9 upper end of hose.Suction ball 11 is ball, can generate negative pressure.
Through-hole 13 is equipped among sealing cover 5, sealing plate 7 and demarcation plate 2, through-hole 13 is sealed by soft seal piece 14, side
Just 10 grafting of hose 9 and check valve, fixation.
The bottom of bottle of culture bottle body 1 is stretched in the end of the hose 9 of the application, and two check valves 10 can be separately fixed at close
At the through-hole 13 of capping 5 and demarcation plate 2, hose 9 is connected with check valve 10 first and is fixed by through-hole 13, is sequentially connected later
Check valve 10, ball.It is easy to use convenient for the bacteria culture bottle of sampling, it is easy to operate, it is not easy to pollute, reusable.
The bacteria culture bottle of the utility model is specifically used, and steps are as follows;
(1) it sterilizes to the various components of the bacteria culture bottle of the application；
(2) whole operation carries out in superclean bench, the liquid training after suitable sterilizing is added in culture bottle body 1
Support base；
(3) target bacteria is seeded in culture medium；
(4) demarcation plate 2 is fixed by fixed block 12；
(5) set bucket 6 is threadedly attached in culture 1 top of bottle body；
(6) check valve 10 and ball that will be connected through hose 9, is put into together in the storage space 8 of sealing cover 5；
(7) sealing cover 5 and set bucket 6 are connected through a screw thread；
(8) assembled culture bottle is put into isothermal vibration device, sets suitable temperature and revolving speed, when cultivating certain
Between；
(9) culture bottle is taken out from oscillator；
(10) sealing cover 5 is backed out, the check valve 10 connected through hose 9 and hose 9, ball are taken out, squeezes emptying rubber
Gas or liquid in ball, make ball be connected to the open end of hose 9, and ball generates negative pressure and draws culture bottle body 1
In culture medium, squeeze ball again, and the culture medium drawn is collected in 9 end of hose；
(11) concentration containing germy culture medium is measured；
(12) check valve 10 and ball that will be connected again through hose 9, is put into together in the storage space 8 of sealing cover 5；
(13) sealing cover 5 and set bucket 6 are connected through a screw thread；
(14) assembled bacteria culture bottle is put into isothermal vibration device, continues to cultivate, until lower sub-sampling.
It should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should manage
Solution: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of skills
Art feature is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution the utility model
The range of each embodiment technical solution.
Claims (8)
1. a kind of bacteria culture bottle convenient for sampling, which is characterized in that including cultivating bottle body (1), set in culture bottle body (1)
Have demarcation plate (2), demarcation plate (2), which will be cultivated, is divided into cultivation region (3) and isolated area (4) inside bottle body (1), cultivate bottle body
(1) top is connected with seal member, and seal member includes sealing cover (5), and set bucket (6) is socketed in sealing cover (5) lower part, covers bucket
(6) internal and culture bottle body (1) is threadedly coupled, and sealing plate (7), sealing plate (7) and sealing cover are equipped with inside sealing cover (5)
(5) top constitutes storage space (8), and one end of hose (9) sequentially passes through sealing cover (5), sealing plate (7) and demarcation plate (2), soft
The interlude and check valve (10) connection, suction ball (11) for managing (9) are connected to hose (9) upper end.
2. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that the culture bottle body
(1) inner wall is equipped with fixed block (12), and demarcation plate (2) is fixed by fixed block (12).
3. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that the suction ball (11) is rubber
Glueballs.
4. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that the sealing cover (5), close
It is equipped with through-hole (13) among sealing plate (7) and demarcation plate (2), through-hole (13) is sealed by soft seal piece (14).
5. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that outside the set bucket (6)
Wall and sealing cover (5) are threadedly coupled, and the inner wall and culture bottle body (1) for covering bucket (6) are threadedly coupled.
6. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that sealing plate (7) water
Flat setting, the external screw thread that sealing plate (7) outer ring is equipped with and sealing cover (5) are mutually matched.
7. a kind of bacteria culture bottle convenient for sampling according to claim 6, which is characterized in that under the sealing plate (7)
Portion is equipped with knob (15), knob (15) hollow setting.
8. a kind of bacteria culture bottle convenient for sampling according to claim 1, which is characterized in that the check valve (10)
Quantity is two, and two check valves (10) can be separately fixed at the through-hole (13) of sealing cover (5) and demarcation plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820713973.3U CN208586299U (en) | 2018-05-15 | 2018-05-15 | A kind of bacteria culture bottle convenient for sampling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820713973.3U CN208586299U (en) | 2018-05-15 | 2018-05-15 | A kind of bacteria culture bottle convenient for sampling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208586299U true CN208586299U (en) | 2019-03-08 |
Family
ID=65533952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820713973.3U Expired - Fee Related CN208586299U (en) | 2018-05-15 | 2018-05-15 | A kind of bacteria culture bottle convenient for sampling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208586299U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110907227A (en) * | 2019-12-03 | 2020-03-24 | 胡双双 | Adjustable sampling device for sewage pool |
-
2018
- 2018-05-15 CN CN201820713973.3U patent/CN208586299U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110907227A (en) * | 2019-12-03 | 2020-03-24 | 胡双双 | Adjustable sampling device for sewage pool |
| CN110907227B (en) * | 2019-12-03 | 2022-12-23 | 胡双双 | Effluent water sump is with adjustable sampling device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190313 Address after: 264006 Chaohui Culture Road East Electronic Industrial Park, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: Shandong one hundred Biotechnology Co., Ltd. Address before: 644300 No. 1-88 Anning Road, Changning County, Yibin City, Sichuan Province Patentee before: Mou Xiaolin |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190308 Termination date: 20200515 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |