CN206970614U - A kind of microorganism fermentation tank classification inoculation apparatus - Google Patents
A kind of microorganism fermentation tank classification inoculation apparatus Download PDFInfo
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- CN206970614U CN206970614U CN201720903902.5U CN201720903902U CN206970614U CN 206970614 U CN206970614 U CN 206970614U CN 201720903902 U CN201720903902 U CN 201720903902U CN 206970614 U CN206970614 U CN 206970614U
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- inoculation
- ball valve
- cap
- fermentation tank
- inoculated
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- 238000011081 inoculation Methods 0.000 title claims abstract description 71
- 238000000855 fermentation Methods 0.000 title claims abstract description 36
- 230000004151 fermentation Effects 0.000 title claims abstract description 36
- 244000005700 microbiome Species 0.000 title claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 22
- 230000000740 bleeding effect Effects 0.000 claims abstract description 14
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 abstract description 10
- 238000011169 microbiological contamination Methods 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000003197 gene knockdown Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
It the utility model is related to a kind of microorganism fermentation tank classification inoculation apparatus, belong to technical field of biological fermentation, the utility model includes fermentation tank, inoculated tube, the first ball valve, connecting tube, the second ball valve, inoculation bottle, steam valve, air bleeding valve, sterilization tray, inoculation cap, handle, the lower end of inoculated tube connects with fermentation tank, the upper end of inoculated tube is sequentially communicated the first ball valve, connecting tube, the second ball valve, inoculation bottle, connecting tube is provided with steam valve close to one end of the first ball valve, and connecting tube is provided with air bleeding valve close to one end of the second ball valve；The middle part on inoculation bottle top is provided with bottleneck, and the bottleneck below bottleneck is provided with sterilization tray, and the outer wall on bottleneck top is provided with screw thread, the madial wall for being inoculated with cap is provided with the screw thread of matching, and inoculation cap is tightly connected with whorl of bottleneck, the top of inoculation cap inner chamber is provided with sealing ring, and inoculation cap is provided with handle；The utility model improves the security of operation, effectively reduces microbiological contamination risk in seeded process, improves success ratio of inoculation.
Description
Technical field
The utility model belongs to technical field of biological fermentation, specifically, is related to a kind of microorganism fermentation tank classification inoculation apparatus.
Background technology
Microbial fermentation needs to cultivate a number of pure microorganism fungus kind under sterile environment before starting.Work as fermentation tank
The culture medium configured is put into, by high-temperature sterilization, when being then cooled to the temperature of technological requirement, ready microbial bacteria
The process that kind is transferred in fermentation tank is to be inoculated with.The operation of inoculation needs to complete under aseptic conditions, otherwise can cause miscellaneous
Bacterium pollution causes fermentation to fail, therefore it is required that classification inoculation apparatus can remain enclosed sterile state in whole seeded process.
In process of production, generally use pyrosphere protection inoculation, after tank pressure drop zero, seed is poured into seeding tank as early as possible.
During inoculation, it is impossible to have air discharge, can not have air flow in environment.Pyrosphere seeded process needs multiple person cooperational to coordinate, to behaviour
It is higher to make personnel requirement.It is depressurized to after flame sterilization in zero this section of process and very big microbiological contamination risk is present, easily fermentation failure,
Cause directly or indirectly economic loss.Meanwhile temperature in fermentation tank and pressure are uncontrollable so that the success rate of inoculation drops significantly
It is low.
The content of the invention
In order to overcome problem present in background technology, the utility model provides a kind of microorganism fermentation tank inoculation dress
Put, improve the security of operation, effectively reduce microbiological contamination risk in seeded process, improve success ratio of inoculation.
To achieve the above object, the utility model is achieved by the following technical solution：
Described microorganism fermentation tank classification inoculation apparatus includes fermentation tank 1, inoculated tube 2, the first ball valve 3, connecting tube 4, second
Ball valve 5, inoculation bottle 6, steam valve 7, air bleeding valve 8, sterilization tray 9, inoculation cap 10, handle 11, the lower end of the inoculated tube 2 and fermentation
Tank 1 connects, and the upper end of inoculated tube 2 is sequentially communicated the first ball valve 3, connecting tube 4, the second ball valve 5, inoculation bottle 6, the connecting tube 4
Steam valve 7 is provided with close to one end of the first ball valve 3, connecting tube 4 is provided with air bleeding valve 8 close to one end of the second ball valve 5；The inoculation
The middle part on 6 tops of bottle is provided with bottleneck 12, and the bottleneck of the lower section of bottleneck 12 is provided with sterilization tray 9, and the outer wall on the top of bottleneck 12 is set
There is screw thread, the madial wall of inoculation cap 10 is provided with the screw thread of matching, and is inoculated with cap 10 and is connected with the thread seal of bottleneck 12, the inoculation
The top of the inner chamber of cap 10 is provided with sealing ring 13, and inoculation cap 10 is provided with handle 11.
Preferably, the handle 11 includes arc interface arrangment 14, turns to hold structure 15, the arc interface arrangment 14
Two free ends are connected located at the outside of inoculation cap 10, and by split pin 16 with inoculation cap 10, described turn hold structure 15 for
The shaft-like structure of the outside integral type connection of arc interface arrangment 14.
Preferably, the bottom of the inoculation bottle 6 is in tapered.
Preferably, first ball valve 3, the second ball valve 5 are without delay ball valve.
The beneficial effects of the utility model：
First ball valve, the second ball valve used in the utility model are without delay ball valve, and it opens and inspect convenient and residual without thalline etc.
Stay, advantageously ensure that sterile, totally enclosed design ensure that the microbiological contamination of seeded process zero, easy to operate.The utility model is provided with
Sterilization tray, a circle alcohol swab can be put into sterilization tray and lights or the pyrosphere lighted is placed in sterilization tray, protected in flame
Assist to complete to be inoculated with without other people down, simple operation, improve security.Before and after inoculation, docked using steam is passed through
Kind pipeline section is sterilized, and the microbiological contamination risk in seeded process is greatly reduced by way of sterilization repeatedly, is effectively ensured
The aseptic inoculation of fermentation tank, improves success ratio of inoculation.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is sectional view of the present utility model；
Fig. 3 is top view of the present utility model.
In figure, 1- fermentation tanks, 2- inoculated tubes, the ball valves of 3- first, 4- connecting tubes, the ball valves of 5- second, 6- inoculation bottles, 7- steam
Valve, 8- air bleeding valves, 9- sterilization trays, 10- inoculations cap, 11- handles, 12- bottlenecks, 13- sealing rings, 14- arcs interface arrangment, 15-
Turn to hold structure, 16- split pins.
Embodiment
In order that the purpose of this utility model, technical scheme and beneficial effect are clearer, below in conjunction with accompanying drawing, to this
The preferred embodiment of utility model is described in detail, to facilitate the technical staff to understand.
As Figure 1-3, described microorganism fermentation tank classification inoculation apparatus include fermentation tank 1, inoculated tube 2, the first ball valve 3,
Connecting tube 4, the second ball valve 5, inoculation bottle 6, steam valve 7, air bleeding valve 8, sterilization tray 9, inoculation cap 10, handle 11.The inoculated tube 2
Lower end connected with fermentation tank 1, the upper end of inoculated tube 2 is sequentially communicated the first ball valve 3, connecting tube 4, the second ball valve 5, inoculation bottle 6,
Connected between inoculated tube 2, the first ball valve 3, connecting tube 4, the second ball valve 5, inoculation bottle 6 by flange seal.Preferably, institute
State the first ball valve 3, the second ball valve 5 be without be detained ball valve, can effectively prevent in bacterium solution residual valve body, avoid valve inner
Strain is polluted in corrosion, ensures the cleaning of ball valve.The connecting tube 4 is provided with steam valve 7, connecting tube 4 close to one end of the first ball valve 3
Air bleeding valve 8 is provided with close to one end of the second ball valve 5, steam valve 7 is opened and is passed through steam, classification inoculation apparatus inside can be carried out at sterilizing
Reason.
The bottom of the inoculation bottle 6 is in tapered, is easy to drop down onto in fermentation tank under strain.The middle part on the top of inoculation bottle 6 is provided with
Bottleneck 12, the bottleneck of the lower section of bottleneck 12 are provided with sterilization tray 9.During inoculation, pyrosphere is placed in sterilization tray, bottleneck is sterilized
Processing, without assisting to complete to be inoculated with, safe and convenient.The outer wall on the top of bottleneck 12 is provided with screw thread, is inoculated with the interior of cap 10
Side wall is provided with the screw thread of matching, and is inoculated with cap 10 and is connected with the thread seal of bottleneck 12.The top of inoculation cap 10 inner chamber is provided with
Sealing ring 13, inoculation cap 10 are provided with handle 11.The handle 11 includes arc interface arrangment 14, turns to hold structure 15, the arc
Two free ends of shape interface arrangment 14 are connected located at the outside of inoculation cap 10, and by split pin 16 with inoculation cap 10, described
It is the shaft-like structure being connected with the outside integral type of arc interface arrangment 14 to turn to hold structure 15.
The course of work of the present utility model：
Before inoculation, during being carried out disinfection for culture medium in fermentation tank, pico- first ball valve 3 of opening flows through steam
Fermentation tank is inoculated with the mouth of pipe, and it is sterilized, and sterilizing terminates to close the first ball valve 3.Then by the first ball valve 3, connecting tube 4,
Two ball valves 5, inoculation bottle 6 are connected with inoculated tube 2, close the first ball valve 3, open steam valve 7 and air bleeding valve 8, lead to sterilizing steam
Cross steam valve 7 and flow through the first ball valve 3, connecting tube 4, the second ball valve 5, inoculation bottle 6, then discharged through air bleeding valve 8, inoculation pipeline section is entered
High-temperature steam sterilization before row inoculation.After completing sterilizing, air bleeding valve 8 and steam valve 7 are closed successively.
Then, a circle alcohol swab is put into sterilization tray 9 to light or the pyrosphere lighted is placed in sterilization tray 9, makes flame
Bottleneck is coated, rotary handle 11 is screwed off cap 10 is inoculated with, and shaking flask strain is transferred into inoculation bottle 6 by bottleneck 12 under flame protection
It is interior, then screw bottleneck 12, knock down the flame with inoculation cap 10.The first ball valve 3 and the second ball valve 5 are opened, will inoculation using static difference pressure
In strain access fermentation tank 1 in bottle 6.After the completion of inoculation, the first ball valve 3 is closed, is again turned on steam valve 7 and air bleeding valve 8, led to
Enter steam again to being inoculated with pipeline section sterilization, after making the first ball valve 3 preferably block the miscellaneous bacteria in external environment, sterilizing to terminate
Air bleeding valve 8 and steam valve 7 are closed successively.
Inoculating device of fermentation tank of the present utility model is greatly reduced in seeded process by way of sterilization repeatedly
Microbiological contamination risk, the aseptic inoculation of fermentation tank 1 has been effectively ensured, has improved success ratio of inoculation.
Finally illustrate, preferred embodiment above is merely to illustrate the technical solution of the utility model and unrestricted, to the greatest extent
The utility model has been described in detail by above preferred embodiment for pipe, but those skilled in the art should manage
Solution, can make various changes, without departing from the utility model claims book institute to it in the form and details
The scope of restriction.
Claims (4)
- A kind of 1. microorganism fermentation tank classification inoculation apparatus, it is characterised in that：Described microorganism fermentation tank classification inoculation apparatus includes fermentation Tank（1）, inoculated tube（2）, the first ball valve（3）, connecting tube（4）, the second ball valve（5）, inoculation bottle（6）, steam valve（7）, air bleeding valve （8）, sterilization tray（9）, inoculation cap（10）, handle（11）, the inoculated tube（2）Lower end and fermentation tank（1）Connection, inoculated tube （2）Upper end be sequentially communicated the first ball valve（3）, connecting tube（4）, the second ball valve（5）, inoculation bottle（6）, the connecting tube（4）Lean on Nearly first ball valve（3）One end be provided with steam valve（7）, connecting tube（4）Close to the second ball valve（5）One end be provided with air bleeding valve（8）； The inoculation bottle（6）The middle part on top is provided with bottleneck（12）, bottleneck（12）The bottleneck of lower section is provided with sterilization tray（9）, the bottle Mouthful（12）The outer wall on top is provided with screw thread, is inoculated with cap（10）Madial wall be provided with matching screw thread, and be inoculated with cap（10）With bottleneck （12）Thread seal connects；The inoculation cap（10）The top of inner chamber is provided with sealing ring（13）, it is inoculated with cap（10）It is provided with handle （11）.
- A kind of 2. microorganism fermentation tank classification inoculation apparatus according to claim 1, it is characterised in that：The handle（11）Including Arc interface arrangment（14）, turn hold structure（15）, the arc interface arrangment（14）Two free ends located at inoculation cap（10） Outside, and pass through split pin（16）With being inoculated with cap（10）Connection, described turn is held structure（15）For with arc interface arrangment（14） The connection of outside integral type shaft-like structure.
- A kind of 3. microorganism fermentation tank classification inoculation apparatus according to claim 1, it is characterised in that：The inoculation bottle（6）'s Bottom is in tapered.
- A kind of 4. microorganism fermentation tank classification inoculation apparatus according to claim 1, it is characterised in that：First ball valve（3）、 Second ball valve（5）It is without delay ball valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720903902.5U CN206970614U (en) | 2017-07-25 | 2017-07-25 | A kind of microorganism fermentation tank classification inoculation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720903902.5U CN206970614U (en) | 2017-07-25 | 2017-07-25 | A kind of microorganism fermentation tank classification inoculation apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206970614U true CN206970614U (en) | 2018-02-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720903902.5U Expired - Fee Related CN206970614U (en) | 2017-07-25 | 2017-07-25 | A kind of microorganism fermentation tank classification inoculation apparatus |
Country Status (1)
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| CN (1) | CN206970614U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641926A (en) * | 2018-05-07 | 2018-10-12 | 张斌行 | A kind of microbial fermentation avoids the system and method for microbiological contamination when inoculation |
| CN114507588A (en) * | 2022-03-08 | 2022-05-17 | 广东天益生物科技有限公司 | Inoculation device and inoculation method for producing monascus color through monascus liquid submerged fermentation |
-
2017
- 2017-07-25 CN CN201720903902.5U patent/CN206970614U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641926A (en) * | 2018-05-07 | 2018-10-12 | 张斌行 | A kind of microbial fermentation avoids the system and method for microbiological contamination when inoculation |
| CN114507588A (en) * | 2022-03-08 | 2022-05-17 | 广东天益生物科技有限公司 | Inoculation device and inoculation method for producing monascus color through monascus liquid submerged fermentation |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180206 |