CN208038437U - It is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device - Google Patents
It is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device Download PDFInfo
- Publication number
- CN208038437U CN208038437U CN201820161164.6U CN201820161164U CN208038437U CN 208038437 U CN208038437 U CN 208038437U CN 201820161164 U CN201820161164 U CN 201820161164U CN 208038437 U CN208038437 U CN 208038437U
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- China
- Prior art keywords
- valve
- aseptic
- inoculation
- differential pressure
- steam pipe
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- 238000011081 inoculation Methods 0.000 title claims abstract description 30
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 23
- 230000001502 supplementing effect Effects 0.000 title claims abstract description 15
- 238000007789 sealing Methods 0.000 claims description 8
- 239000006052 feed supplement Substances 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 7
- 238000001354 calcination Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The utility model discloses in differential pressure inoculation and feed supplement field it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, including aseptic bottle and aseptic tank, valve one is connected on the aseptic bottle, the valve one is connected with valve two, valve three is provided on the aseptic tank, the valve one is connected by steam pipe one with valve three, and the valve three is connected with valve four, the valve four is connected with steam pipe two, and valve five is provided on the aseptic tank;Pass through the connection that aseptic bottle and aseptic tank are passed through into steam pipe and valve; ensure that aseptic bottle, aseptic tank and the pipeline connected between the two and valve can sterilize in moise-heat sterilization online; it is protected without flame; ensure the safe and reliable of seeded process; avoid operating personnel by flame calcination, the utility model can be used among sterile pressure difference inoculation and feed supplement.
Description
Technical field
The utility model is related to a kind of disinfection inoculation and feed supplementing device, more particularly to it is a kind of can online steam sterilizing differential pressure
Inoculation and feed supplementing device.
Background technology
Before microbial fermentation starts, needs to cultivate a certain number of pure microorganism fungus kinds under sterile environment, work as life
Object reactor(Or fermentation tank)It puts into the culture medium configured and is then cooled to the temperature of technological requirement by high-temperature sterilization
When, it is inoculation or feed supplement that ready microorganism fungus kind, which is transferred to the process in bioreactor,.The behaviour of inoculation or feed supplement
Need to complete under aseptic conditions, it is no it will cause living contaminants so as to cause fermentation fail, therefore just need be inoculated with
Feed supplementing device inoculation or material-feeding port around generate gnotobasis for inoculation or feed operation smoothly complete.It is sterile at present
Inoculation or feed supplement mostly use greatly the inoculation of flame differential pressure or feed supplement method, i.e., by inoculation or feed supplement pipe and aseptic tank under flame protection
Inoculation or material-feeding port are connected, it is done so that can not ensure that inoculation or feed supplement process are sterile completely, and operating personnel are easily by fire
Flame is burnt.
Utility model content
The purpose of this utility model be to provide it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, by by nothing
Bacterium bottle and the aseptic tank connection that passes through steam pipe and valve, it is ensured that aseptic bottle, aseptic tank and the pipeline connected between the two and
Valve can online moise-heat sterilization sterilizing, protected without flame, it is ensured that inoculation or feed supplement process it is safe and reliable, avoid operating
Personnel are by flame calcination.
To achieve the above object, the utility model provide it is a kind of can online steam sterilizing differential pressure inoculation and feed supplement dress
It sets, including aseptic bottle and aseptic tank, valve one is connected on the aseptic bottle, the valve one is connected with valve two, the nothing
Valve three is provided on bacterium tank, the valve one is connected by steam pipe one with valve three, and the valve three is connected with valve four,
The valve four is connected with steam pipe two, and valve five is provided on the aseptic tank.
Compared with prior art, the beneficial effects of the utility model are, by the way that aseptic bottle and aseptic tank are passed through steam
The connection of pipe and valve, it is ensured that aseptic bottle, aseptic tank and the pipeline connected between the two and valve can online moise-heat sterilizations
Sterilizing is protected without flame, it is ensured that inoculation or feed supplement process it is safe and reliable, avoid operating personnel by flame calcination, this
Utility model can be used for having sterile pressure difference be inoculated with or feed supplement among.
The steam pipe one and steam pipe two are pressure hose as a further improvement of the utility model, in this way can be with
So that steam pipe installing and dismounting is more convenient, and ensure that steam pipe can bear larger pressure, avoids because pressure is excessive
And it damages.
The steam pipe one is connected by sealing chuck one with valve one as a further improvement of the utility model, institute
It states steam pipe one and is connected with valve three by sealing chuck two, can be ensured between steam pipe one and valve one and valve three in this way
Connection it is more solid and reliable, the leakage and inoculation or the microbiological contamination caused by leakage when feed supplement of steam when avoiding disinfection.
The valve one is connected by the lower part of connecting tube and aseptic bottle as a further improvement of the utility model, institute
The support element for being provided in connecting tube and being connect with aseptic bottle is stated, can allow the seed liquor in aseptic bottle complete rapidly in this way
Ground flows into aseptic tank, and connecting tube is supported effective support of part, strengthens the structural strength of connecting tube, avoids pressure
Excessive shakes.
The valve three is connect with the top of aseptic tank as a further improvement of the utility model, in this way can be to avoid
Cause seed liquor that cannot fully flow into the phenomenon in aseptic tank because valve three and sterile tank connected position are too low.
The valve one and valve three are DN15 diaphragm valves, the valve two as a further improvement of the utility model,
It is DN8 diaphragm valves with valve four, not only ensure that better seal also ensures the pure of seed liquor and circulation more in this way
Add smooth.
Description of the drawings
FIG. 1 is a schematic structural view of the utility model.
Wherein, 1 aseptic bottle, 2 support elements, 3 connecting tubes, 4 valves one, 5 sealing chucks one, 6 valves two, 7 steam pipes one, 8
Steam pipe two, 9 valves four, 10 valves three, 11 sealing chucks two, 12 aseptic tanks, 13 valves five.
Specific implementation mode
The utility model is further illustrated below in conjunction with the accompanying drawings:
Embodiment 1
It is as shown in Figure 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, including aseptic bottle 1 and aseptic tank
12, valve 1 is connected on aseptic bottle 1, and valve 1 is connected with valve 26, and valve 3 10, valve are provided on aseptic tank 12
One 4 are connected by steam pipe 1 with valve 3 10, and valve 3 10 is connected with valve 49, and valve 49 is connected with steam pipe 28,
Valve 5 13 is provided on aseptic tank 12;Steam pipe 1 and steam pipe 28 are pressure hose;Steam pipe 1 is by sealing chuck
One 5 are connected with valve 1, and steam pipe 1 is connected by sealing chuck 2 11 with valve 3 10;Valve 1 by connecting tube 3 with
The lower part of aseptic bottle 1 connects, and the support element 2 being connect with aseptic bottle 1 is provided in connecting tube 3;Valve 3 10 and aseptic tank 12
Top connects;Valve 1 is DN15 diaphragm valves with valve 3 10, and valve 26 is DN8 diaphragm valves with valve 49.
When needing to carry out aseptic inoculation, aseptic bottle 1 is seed bottle, and when aseptic bottle 1 is in desinfection chamber, valve 1 is
Closed state is closed state in 12 medium sterilization late gate 3 10 of aseptic tank, and unilateral side of the valve 26 for valve 1 is small
Exhaust, unilateral side small exhaust of the valve 49 for valve 3 10, steam pipe 28 connect steam, open valve 49 and valve 26,
Valve 1 and valve 3 10 are closed states, and steam passes sequentially through valve 3 10, steam pipe 1, valve 1 from valve 49,
It is finally discharged from valve 26, steam sterilizing is carried out 10 ~ 20 minutes to valve and steam pipe, after sterilizing, steam off closes
Valve closing door 49 and valve 26 open valve 3 10 and valve 1, close valve 5 13 and boost, pressure rise is extremely
At this moment 0.1MPa opens 5 13 rapid pressure release of valve, using differential pressure, so that the seed liquor in aseptic bottle 1 is flowed into aseptic tank 12, instead
Boosting pressure discharge operations 2 ~ 3 times again make in aseptic bottle 1 after seed liquor whole inflow aseptic tank 12, and closing valve 3 10 opens valve
26, after making device pressure release, steam pipe 1 is removed, completes inoculate flow process.
Embodiment 2
It is as described in Figure 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, be the another of the utility model
Kind embodiment, when needing to carry out asepsis feeding, aseptic bottle 1 is feed supplement bottle, remaining structure does not change, and the course of work is not
Work changes, and can be used among sterile pressure difference feed supplement.
The utility model is not limited to the above embodiment, on the basis of the technical solution of the disclosure, the technology of this field
According to disclosed technology contents, some of which technical characteristic can be made personnel by not needing performing creative labour
It replaces and deformation, these replacements and deformation is within the protection scope of the present utility model.
Claims (6)
1. it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Including aseptic bottle and aseptic tank, institute
It states and is connected with valve one on aseptic bottle, the valve one is connected with valve two, and valve three, the valve are provided on the aseptic tank
Door one is connected by steam pipe one with valve three, and the valve three is connected with valve four, and the valve four is connected with steam pipe two,
Valve five is provided on the aseptic tank.
2. it is according to claim 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Institute
It is pressure hose to state steam pipe one and steam pipe two.
3. it is according to claim 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Institute
It states steam pipe one and is connected with valve one by sealing chuck one, the steam pipe one is connected by sealing chuck two with valve three.
4. it is according to claim 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Institute
It states valve one to connect by the lower part of connecting tube and aseptic bottle, the support element being connect with aseptic bottle is provided in the connecting tube.
5. it is according to claim 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Institute
Valve three is stated to connect with the top of aseptic tank.
6. it is according to claim 1 it is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device, it is characterised in that:Institute
It is DN15 diaphragm valves that valve one, which is stated, with valve three, and the valve two is DN8 diaphragm valves with valve four.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820161164.6U CN208038437U (en) | 2018-01-31 | 2018-01-31 | It is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820161164.6U CN208038437U (en) | 2018-01-31 | 2018-01-31 | It is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208038437U true CN208038437U (en) | 2018-11-02 |
Family
ID=63955025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820161164.6U Active CN208038437U (en) | 2018-01-31 | 2018-01-31 | It is a kind of can online steam sterilizing differential pressure inoculation and feed supplementing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208038437U (en) |
-
2018
- 2018-01-31 CN CN201820161164.6U patent/CN208038437U/en active Active
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