CN207418737U - A kind of classification inoculation apparatus of microorganism fermentation tank - Google Patents
A kind of classification inoculation apparatus of microorganism fermentation tank Download PDFInfo
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- CN207418737U CN207418737U CN201721217036.0U CN201721217036U CN207418737U CN 207418737 U CN207418737 U CN 207418737U CN 201721217036 U CN201721217036 U CN 201721217036U CN 207418737 U CN207418737 U CN 207418737U
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- 238000011081 inoculation Methods 0.000 title claims abstract description 133
- 238000000855 fermentation Methods 0.000 title claims abstract description 82
- 230000004151 fermentation Effects 0.000 title claims abstract description 82
- 244000005700 microbiome Species 0.000 title claims abstract description 25
- 238000007789 sealing Methods 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims description 19
- 230000008676 import Effects 0.000 claims description 10
- 208000026231 acute otitis externa Diseases 0.000 claims 1
- 201000011645 beach ear Diseases 0.000 claims 1
- 238000009434 installation Methods 0.000 claims 1
- 238000011169 microbiological contamination Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 description 16
- 230000001954 sterilising effect Effects 0.000 description 16
- 238000004659 sterilization and disinfection Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 239000000284 extract Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model is related to a kind of classification inoculation apparatus of microorganism fermentation tank, including fermentation tank inoculation mouth, inoculation tank, the first inoculated tube, the second inoculated tube and inoculating hood, inoculating hood is mounted on the top of fermentation tank inoculation mouth, and inoculation tank with first the second inoculated tube of inoculated tube by accessing in fermentation tank inoculation mouth；Sealing ring and duckbill valve are sequentially installed in fermentation tank inoculation mouth, exhaust outlet is provided on the fermentation tank inoculation mouth tube wall between sealing ring and inoculating hood, one end of the first inoculated tube is equipped with air inlet；It is inoculated with above the both sides of tank and is equipped with hangers；Lower section is equipped with supporting leg；Inoculating hood is the double cylinder case for being provided with centre bore, and case is equipped with ultraviolet lamp.The classification inoculation apparatus of the microorganism fermentation tank of the utility model is simple in structure, it is easy to operate, can quickly be inoculated with, not easily cause microbiological contamination.
Description
Technical field
The utility model belongs to biological fermentation field, and in particular to a kind of classification inoculation apparatus of microorganism fermentation tank.
Background technology
Microbial fermentation needs to cultivate a certain number of pure microorganism fungus kinds under sterile environment before starting.Work as fermentation tank
The culture medium that (or bioreactor) input has configured, by high-temperature sterilization, when being then cooled to the temperature of technological requirement, standard
The process that the microorganism fungus kind got ready is transferred in fermentation tank is to be inoculated with.The operation of inoculation needs complete under aseptic conditions
Into, it is no it will cause living contaminants to cause fermentation fail, therefore it is required that classification inoculation apparatus can be protected always in entire seeded process
Hold enclosed sterile state.
The inoculation method often used on fermentation tank mainly has flame inoculation method.Flame inoculation method is usually to be connect in fermentation tank
Combustible is placed around kind mouth, the flame that combustible generates after being ignited makes to form sterile subenvironment around inoculation mouth,
Aseptic inoculation operation is carried out in this subenvironment.The defects of flame inoculation method is：First, the opening of inoculation bottle and fermentation tank inoculation mouth
It must be placed in always under the protection of flame, if misoperation or bradypragia, strain exposure may all be caused to contaminate in air
Bacterium；Second, flame temperature is very high, and inoculation easily causes hand when operating and burns.Therefore, how to be made a kind of easy to operate, be not easy
The classification inoculation apparatus of microbiological contamination is the current technical issues that need to address.
The content of the invention
The purpose of this utility model is for above deficiency, provides a kind of classification inoculation apparatus of microorganism fermentation tank.This reality
With the classification inoculation apparatus of new microorganism fermentation tank it is simple in structure, it is easy to operate, can quickly be inoculated with, do not easily cause microbiological contamination.
To achieve the above object, the utility model adopts the following technical solution：
A kind of classification inoculation apparatus of microorganism fermentation tank connects including fermentation tank inoculation mouth, inoculation tank, the first inoculated tube, second
Kind pipe and inoculating hood, the inoculating hood are mounted on the top of fermentation tank inoculation mouth, and the inoculation tank is first connected with the first inoculated tube,
Then the first inoculated tube is connected with the second inoculated tube by flange, and the second inoculated tube is then passed through inoculating hood and enters fermentation tank inoculation mouth
It is interior；Sealing ring and duckbill valve are sequentially installed in the fermentation tank inoculation mouth, wherein, sealing ring is closer to inoculating hood, sealing ring
Exhaust outlet is provided on fermentation tank inoculation mouth tube wall between inoculating hood, first inoculated tube is set close to one end of inoculation tank
There is air inlet；The top of the inoculation tank is equipped with inoculation import, is inoculated in import and is equipped with inoculation access cover, the inoculation tank
Lower section is equipped with liquid outlet, and control switch is equipped at liquid outlet；Hangers is equipped with above the both sides of the inoculation tank；Lower section is equipped with
Supporting leg；The inoculating hood is the double cylinder case for being provided with centre bore, and case is equipped with ultraviolet lamp.
Height is equipped in the first inoculated tube and fermentation tank inoculation mouth of the classification inoculation apparatus of the microorganism fermentation tank of the utility model
Warm steam inlet and exhaust outlet provide sterilization passage, effective sterilizing；
Meanwhile two layers of sealing valve is equipped in the fermentation tank inoculation mouth of the utility model（Sealing ring and duckbill valve）, it is inoculated
Cheng Zhong, the second inoculated tube sequentially passes through sealing ring and duckbill valve is connected with fermentation tank, since duckbill valve is that opening is flat mouth shape
（Yi word pattern）, when the second inoculated tube passes through, duckbill valve flat mouth is opened, and is had a small amount of gap between the two, is connect at this time with second
The sealing ring of kind pipe connection just plays the role of secondary seal, and the leakproofness in seeded process can be effectively ensured, prevent from contaminating
Bacterium, meanwhile, after the completion of inoculation, extract the second inoculated tube out from sealing valve, duckbill valve can close immediately, will be inside fermentation tank and outer
Boundary directly completely cuts off, and also substantially plays the effect for preventing microbiological contamination.
Finally, hangers is set on the inoculation tank of the utility model, the height of inoculation tank according to inoculation needs, can be improved,
Ensure enough liquid level differences, it is ensured that the liquid in inoculation tank can be flowed rapidly into fermentation tank.
Ultraviolet lamp is provided on the inoculating hood of the utility model, it may have sterilization functions are sterilized with high-temperature vapor and coordinated,
Sterilization effect is further improved, prevents external contamination source and enters fermentation tank.
As preferred technical solution：
Preferably, wheel is installed on the supporting leg.Belt wheel supporting leg is additionally provided on inoculation tank, facilitates the transfer of inoculation tank
Preferably, the air inlet is equipped with switch, also is provided with switching on the exhaust outlet.
Preferably, inoculation access cover is equipped with by screw thread in the inoculation import；It is connected on the inoculation access cover
Handle.
Preferably, what is be passed through in the air inlet is high-temperature vapor.
Preferably, the ultraviolet lamp is mounted on the bottom surface of inoculating hood.
The use process of the classification inoculation apparatus of the microorganism fermentation tank of the utility model is, first will inoculation tank, the first inoculated tube,
Second inoculated tube, inoculating hood, fermentation tank inoculation mouth are sequentially connected, and the second inoculated tube is stretched into fermentation tank inoculation mouth but is located at this time
The top of duckbill valve（It is not connected with fermentation tank）, high-temperature vapor then is passed through from the air inlet on the first inoculated tube, to
Space in one inoculated tube and the second inoculated tube and fermentation tank inoculation mouth sterilizes, and the ultraviolet lamp on inoculating hood is also opened simultaneously
It opens, it is common to sterilize, after the completion of sterilizing, after the second inoculated tube is stretched into duckbill valve, the control of inoculation tank liquid outlet can be opened
Switch, is inoculated with, after seeded process, if due to inoculation tank position it is too low caused by liquid level difference it is less when, duckbilled can be lifted
The hangers of valve improves liquid level, ensures being smoothed out for inoculation discharge opeing process；After the completion of inoculation, the second inoculated tube, duckbill valve are extracted out
It will directly completely cut off with extraneous inside fermentation tank immediately, also substantially play the effect for preventing microbiological contamination.
Advantageous effect
Height is equipped in the first inoculated tube and fermentation tank inoculation mouth of the classification inoculation apparatus of the microorganism fermentation tank of the utility model
Warm steam inlet and exhaust outlet provide sterilization passage, effective sterilizing；
Meanwhile two layers of sealing valve is equipped in the fermentation tank inoculation mouth of the utility model（Sealing ring and duckbill valve）, it is inoculated
Cheng Zhong, the second inoculated tube sequentially passes through sealing ring and duckbill valve is connected with fermentation tank, since duckbill valve is that opening is flat mouth shape
（Yi word pattern）, when the second inoculated tube passes through, duckbill valve flat mouth is opened, and is had a small amount of gap between the two, is connect at this time with second
The sealing ring of kind pipe connection just plays the role of secondary seal, and the leakproofness in seeded process can be effectively ensured, prevent from contaminating
Bacterium, meanwhile, after the completion of inoculation, extract the second inoculated tube out from sealing valve, duckbill valve can close immediately, will be inside fermentation tank and outer
Boundary directly completely cuts off, and also substantially plays the effect for preventing microbiological contamination.
Finally, hangers is set on the inoculation tank of the utility model, the height of inoculation tank according to inoculation needs, can be improved,
Ensure enough liquid level differences, it is ensured that the liquid in inoculation tank can be flowed rapidly into fermentation tank.
Ultraviolet lamp is provided on the inoculating hood of the utility model, it may have sterilization functions are sterilized with high-temperature vapor and coordinated,
Sterilization effect is further improved, prevents external contamination source and enters fermentation tank.
Description of the drawings
Fig. 1 is the structure diagram of the classification inoculation apparatus of the microorganism fermentation tank of the utility model；
Fig. 2 is the structure diagram before the microorganism fermentation tank fermentation tank inoculation mouth inoculation of the utility model；
Structure diagram when Fig. 3 is the microorganism fermentation tank fermentation tank inoculation mouth inoculation of the utility model；
Wherein, 1- is inoculated with tank, 11- inoculation imports, 12- liquid outlets, 13- inoculation access covers, 14- hangers, 15- supporting legs, 2-
First inoculated tube, 21- air inlets, the second inoculated tubes of 3-, 4- fermentation tank inoculation mouths, 41- exhaust outlets, 5- inoculating hoods, 51- are ultraviolet
Lamp, 6- sealing rings, 7- duckbill valves.
Specific embodiment
The following further describes the utility model with specific embodiments.
As illustrated in the accompanying drawings from 1 to 3, it is a kind of classification inoculation apparatus of microorganism fermentation tank of the utility model, including fermentation tank inoculation mouth
4th, tank 1, the first inoculated tube 2, the second inoculated tube 3 and inoculating hood 5 are inoculated with, inoculating hood 5 is mounted on the top of fermentation tank inoculation mouth 4,
Inoculation tank 1 is first connected with the first inoculated tube 2, and then the first inoculated tube 2 is connected with the second inoculated tube 3 by flange, the second inoculation
Pipe 3 is then passed through inoculating hood 5 and enters in fermentation tank inoculation mouth 4；Sealing ring 6 and duckbill valve 7 are sequentially installed in fermentation tank inoculation mouth 4
（Sealing ring 6 and duckbill valve 7 select rubber material）, wherein, sealing ring 6 closer to inoculating hood 5, sealing ring 6 and inoculating hood 5 it
Between 4 tube wall of fermentation tank inoculation mouth on be provided with exhaust outlet 41, the first inoculated tube 2 is equipped with air inlet close to one end of inoculation tank 1
21；The top for being inoculated with tank 1 is equipped with inoculation import 11, is inoculated in import 11 and is equipped with inoculation access cover 13, and the lower section of inoculation tank 1 is set
There is liquid outlet 12, control switch is equipped at liquid outlet 12；It is inoculated with above the both sides of 1 tank body of tank and is equipped with hangers 14；Lower section is equipped with branch
Leg 15；Inoculating hood 5 is the double cylinder case for being provided with centre bore, and case is equipped with ultraviolet lamp 51.
It is equipped in the first inoculated tube 2 and fermentation tank inoculation mouth 4 of the classification inoculation apparatus of the microorganism fermentation tank of the utility model
High-temperature steam air inlet 21 and exhaust outlet 41, provide sterilization passage, effective sterilizing；
Meanwhile two layers of sealing valve is equipped in the fermentation tank inoculation mouth 4 of the utility model（Sealing ring 6 and duckbill valve 7）, inoculation
In the process, the second inoculated tube 3 sequentially passes through sealing ring 6 and duckbill valve 7 is connected with fermentation tank, since duckbill valve 7 is that opening is
Flat mouth shape（Yi word pattern）, when the second inoculated tube 3 passes through, 7 flat mouth of duckbill valve is opened, and has a small amount of gap between the two, at this time
The sealing ring 6 being connected with the second inoculated tube 3 just plays the role of secondary seal, and the sealing in seeded process can be effectively ensured
Property, prevent microbiological contamination, meanwhile, after the completion of inoculation, extract the second inoculated tube 3 out from sealing valve, duckbill valve 7 can be closed immediately, will be sent out
Directly completely cut off with extraneous inside fermentation tank, also substantially play the effect for preventing microbiological contamination.
Finally, hangers 14 on the inoculation tank 1 of the utility model is set, the height of inoculation tank 1 according to inoculation needs, can be improved
Degree, ensures enough liquid level differences, it is ensured that the liquid in inoculation tank 1 can be flowed rapidly into fermentation tank.
Ultraviolet lamp 51 is provided on the inoculating hood 5 of the utility model, it may have sterilization functions are matched somebody with somebody with high-temperature vapor sterilization
It closes, further improves sterilization effect, prevent external contamination source and enter fermentation tank.
Preferably, wheel is installed on supporting leg.Belt wheel supporting leg is additionally provided on inoculation tank 1, facilitates the transfer of inoculation tank 1
Preferably, air inlet 21 is equipped with switch, also is provided with switching on exhaust outlet 41.
Preferably, it is inoculated in import 11 and inoculation access cover 13 is equipped with by screw thread；Hand is connected on inoculation access cover 13
Handle.
Preferably, what is be passed through in air inlet 21 is high-temperature vapor.
Preferably, ultraviolet lamp 51 is mounted on the bottom surface of inoculating hood 5.
The use process of the classification inoculation apparatus of the microorganism fermentation tank of the utility model is that will first be inoculated with tank 1, the first inoculated tube
2nd, the second inoculated tube 3, inoculating hood 5, fermentation tank inoculation mouth 4 are sequentially connected, and the second inoculated tube 3 stretches into fermentation tank inoculation mouth 4 at this time
Top interior but positioned at duckbill valve 7（It is not connected with fermentation tank）, then high temperature is passed through from the air inlet 21 on the first inoculated tube 2
Vapor sterilizes to the space in the first inoculated tube 2 and the second inoculated tube 3 and fermentation tank inoculation mouth 4, on inoculating hood 5
Ultraviolet lamp 51 also open simultaneously, it is common to sterilize, after the completion of sterilizing, after the second inoculated tube 3 is stretched into duckbill valve 7, Bian Kekai
Open the control switch of inoculation 1 liquid outlet 12 of tank, be inoculated with, after seeded process, if due to inoculation tank 1 position it is too low caused by
When liquid level difference is less, the hangers 14 of duckbill valve 7 can be lifted, improve liquid level, ensure being smoothed out for inoculation discharge opeing process；It has been inoculated with
Cheng Hou extracts the second inoculated tube 3 out, and duckbill valve 7 will directly completely cut off inside fermentation tank with extraneous immediately, and also substantially playing prevents microbiological contamination
Effect.
Claims (6)
1. a kind of classification inoculation apparatus of microorganism fermentation tank, it is characterized in that：Including fermentation tank inoculation mouth, inoculation tank, the first inoculated tube,
Second inoculated tube and inoculating hood, the inoculating hood are mounted on the top of fermentation tank inoculation mouth, and the inoculation tank is first inoculated with first
Pipe is connected, and then the first inoculated tube is connected with the second inoculated tube by flange, and the second inoculated tube is then passed through inoculating hood and enters fermentation
In tank inoculation mouth；Sealing ring and duckbill valve are sequentially installed in the fermentation tank inoculation mouth, wherein, sealing ring is closer to inoculation
It covers, exhaust outlet is provided on the fermentation tank inoculation mouth tube wall between sealing ring and inoculating hood, first inoculated tube is close to inoculation
One end of tank is equipped with air inlet；The top of the inoculation tank is equipped with inoculation import, is inoculated in import and is equipped with inoculation access cover, institute
The lower section for stating inoculation tank is equipped with liquid outlet, and control switch is equipped at liquid outlet；It is equipped with and hangs above the both sides of the inoculation tank
Ear；Lower section is equipped with supporting leg；The inoculating hood is the double cylinder case for being provided with centre bore, and case is equipped with ultraviolet lamp.
2. the classification inoculation apparatus of a kind of microorganism fermentation tank according to claim 1, which is characterized in that installed on the supporting leg
There is wheel.
3. the classification inoculation apparatus of a kind of microorganism fermentation tank according to claim 1, which is characterized in that set on the air inlet
There is switch, also be provided with switching on the exhaust outlet.
4. the classification inoculation apparatus of a kind of microorganism fermentation tank according to claim 1, which is characterized in that in the inoculation import
Inoculation access cover is equipped with by screw thread；Handle is connected on the inoculation access cover.
5. the classification inoculation apparatus of a kind of microorganism fermentation tank according to claim 1, which is characterized in that lead in the air inlet
What is entered is vapor.
A kind of 6. classification inoculation apparatus of microorganism fermentation tank according to claim 1, which is characterized in that the ultraviolet lamp installation
On the bottom surface of inoculating hood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721217036.0U CN207418737U (en) | 2017-09-21 | 2017-09-21 | A kind of classification inoculation apparatus of microorganism fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721217036.0U CN207418737U (en) | 2017-09-21 | 2017-09-21 | A kind of classification inoculation apparatus of microorganism fermentation tank |
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| Publication Number | Publication Date |
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| CN207418737U true CN207418737U (en) | 2018-05-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721217036.0U Active CN207418737U (en) | 2017-09-21 | 2017-09-21 | A kind of classification inoculation apparatus of microorganism fermentation tank |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669659A (en) * | 2019-07-05 | 2020-01-10 | 青海华牛生物科技有限公司 | A kind of wolfberry enzyme feed liquid inoculation tank |
| CN113444622A (en) * | 2021-06-25 | 2021-09-28 | 江苏省奥谷生物科技有限公司 | Closed is from sterile fermentation cylinder inoculation system |
| CN114480105A (en) * | 2022-02-14 | 2022-05-13 | 苗新宇 | Microbial fermentation tank auxiliary inoculation sterilization cover |
-
2017
- 2017-09-21 CN CN201721217036.0U patent/CN207418737U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669659A (en) * | 2019-07-05 | 2020-01-10 | 青海华牛生物科技有限公司 | A kind of wolfberry enzyme feed liquid inoculation tank |
| CN113444622A (en) * | 2021-06-25 | 2021-09-28 | 江苏省奥谷生物科技有限公司 | Closed is from sterile fermentation cylinder inoculation system |
| CN113444622B (en) * | 2021-06-25 | 2024-01-09 | 江苏省奥谷生物科技有限公司 | Closed self-disinfection fermentation tank inoculation system |
| CN114480105A (en) * | 2022-02-14 | 2022-05-13 | 苗新宇 | Microbial fermentation tank auxiliary inoculation sterilization cover |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 300457 Tianjin Binhai high tech Zone, Tanggu marine science and Technology Park, Xiamen road 2938 Patentee after: TIANJIN KUNHE BIOLOGICAL GROUP Co.,Ltd. Address before: 300457 Tianjin Binhai New Area Tianjin Binhai high tech Zone Tanggu marine science and Technology Park No. 2938 Xiamen road. Patentee before: TIANJIN KUNHE BIO TECHNOLOGY CO.,LTD. |
|
| CP03 | Change of name, title or address | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: An inoculation device for microbial fermentation tanks Effective date of registration: 20230712 Granted publication date: 20180529 Pledgee: China Construction Bank Corporation Tianjin Development Branch Pledgor: TIANJIN KUNHE BIOLOGICAL GROUP Co.,Ltd. Registration number: Y2023120000066 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |