CN207330923U - A kind of microorganism produces the collection device of gas - Google Patents
A kind of microorganism produces the collection device of gas Download PDFInfo
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- CN207330923U CN207330923U CN201720823308.5U CN201720823308U CN207330923U CN 207330923 U CN207330923 U CN 207330923U CN 201720823308 U CN201720823308 U CN 201720823308U CN 207330923 U CN207330923 U CN 207330923U
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Abstract
A kind of collection device for the gas that microorganism produces is the utility model is related to, including with graduated test tube 1, the seal cap 2 of built-in gasket, soft air-guide pipe 4, check valve 5, soft air-guide pipe 6, collection airbag 7.1 mouth of pipe of test tube is equipped with external screw thread, and test tube is in cylinder；For the seal cap 2 with test tube 1 using being threadedly coupled, a built-in gasket, seal cap top is equipped with hard air outlet pipe；The soft air-guide pipe 4 connects check valve 5 with one end of soft air-guide pipe 6, and check valve 5 controls the one-way flow of whole device gas；Soft air-guide pipe 6 is hermetically connected at 7 air inlet of collection airbag.The utility model also provides a kind of application method of above-mentioned gas collection device, and fluid nutrient medium is poured into test tube and is quantified using scale；It is inoculated with certain microorganism or the sample containing microorganism；Seal cap is connected with test tube, and screwing hermetic cap；Seal cap gas outlet and 4 one end of soft air-guide pipe are socketed, 4 other end of soft air-guide pipe is socketed with check valve inlet；Unidirectional valve air gate and soft air-guide pipe 6 are socketed, soft air-guide pipe 6 is socketed with collection airbag air inlet pipe, test tube, check valve, collection airbag is connected composition gas collecting apparatus by soft air-guide pipe；Gas collecting apparatus is put into incubator and is cultivated, and collects gas；It is automatically sealed in after the completion of gas collecting in collection airbag.
Description
Technical field
The present invention relates to a kind of collection device, and in particular to a kind of aerogen culture and gas collector.
Background technology
Microorganism decomposes during growth and breeding or produces gas using Cucumber, in the research process of microorganism
In, collect and the gas of observation microorganism generation is a kind of widely used method.
Traditional microculture and gas collection method is that an inverted Du Shi tubule is put into equipped with Liquid Culture
In the test tube of base, hydraulically full culture medium in Du Shi tubules after sterilizing.A certain amount of microorganism is accessed into test tube, it is suitable to be placed in
Cultivated under temperature conditionss, there is bubble-free to judge whether microorganism has aerogenesis energy by observing in Du Shi tubules after culture
Power, the method is referred to as " the small tube method of Du Shi ".
Above-mentioned microculture and aerogenesis collection device has some shortcomings：（1）The culture medium of dress in test tube goes out
Gas may not drained in Du Shi tubules after bacterium, there is minute bubbles, and remaining minute bubbles may have the judgement of experimental result
Interference；（2）When certain microorganism gas deliverability is weaker, Du Shi tubules possibly can not be collected into gas；（3）Du Shi tubules can be received
The portion gas collected is limited and is not easy to take out carry out qualitative and quantitative analysis, for needing certain micro- life of qualitative and quantitative analysis
It is unappeasable using " the small tube method of Du Shi " when the quantity and gas componant of thing institute aerogenesis body.
The content of the invention
It is collected the technical problem to be solved in the present invention is to provide a kind of gas that can be produced completely to microorganism
Gas collecting apparatus and application method, can carry out qualitative and quantitative analysis, so as to overcome existing apparatus not to the gas that microorganism produces
Foot.
In order to solve the above technical problems, the present invention provides a kind of collection device of the gas of microculture and generation, bag
Include and be equipped with graduated test tube, the seal cap of built-in sealing ring, check valve, soft air-guide pipe, collection airbag, the test tube mouth of pipe
External screw thread, test tube is in cylinder；The seal cap is utilized with test tube and is threadedly coupled, a built-in sealing ring, at the top of seal cap
Equipped with hard air outlet pipe；Connection check valve among the soft air-guide pipe, controls the one-way flow of whole device gas；Described
Soft air-guide pipe is hermetically connected at collection airbag air inlet pipe.
The test tube outer wall is equipped with scale, and easy to the quantitative adding liquid culture medium into test tube, test tube is in cylinder, bottom
Portion is plane.
The test tube mouth of pipe is equipped with external screw thread, and the seal cap is equipped with interior spiral shell corresponding with test tube mouth of pipe external screw thread
Line.
The seal cap sets internal thread, a built-in sealing ring, for sealing test tube.
The seal cap center of top is equipped with a gas outlet, connects a hard air outlet pipe gas outlet seal, goes out
It is socketed between tracheae and check valve with a soft air-guide pipe.
Piece soft air-guide pipe other end connection check valve, the unidirectional stream of gas in gas collecting apparatus is controlled by check valve
It is dynamic.
Spring and needle are equipped with the check valve, the needle is oriented to equipped with circular cone, in non-return valve operational process
In by circular cone guide gas to flow into collection airbag through another soft air-guide pipe.
The collection airbag air inlet is equipped with plastic conduit as air inlet pipe, is used between check valve and collection airbag air inlet pipe
Another soft air-guide pipe connection.
The graduated test tube, using being threadedly coupled, is sealed with seal cap using gasket；Seal cap air outlet pipe and one
Root soft air-guide pipe is socketed；The soft air-guide pipe other end is socketed with check valve inlet, unidirectional valve air gate and another soft air-guide pipe
Socket；The soft air-guide pipe other end is socketed with collection airbag air inlet pipe.Each connecting portion of soft air-guide pipe uses interference fit seal.
The graduated test tube, check valve valve body use polyvinyl chloride material；Soft air-guide pipe uses polyethylene material；Sealing
Pad uses polytetrafluoroethylene (PTFE) material；Collection airbag uses foil laminated film material.Package unit uses ethylene oxide sterilizing.
As a further improvement, in the step, should connect in seal cap and test tube junction, check valve valve body and valve deck
Connect place's installation gasket.
A kind of application method of the collection device of the gas produced the present invention also provides mentioned microorganism, including following step
Suddenly：A. it is spare after gas collecting apparatus sterilization treatment；B. aseptic liquid nutrient medium is quantified and poured into test tube；C. it is inoculated with tested certain
Microorganism or microbiological specimens；D. seal cap is connected with test tube, and screwing hermetic cap；E. seal cap gas outlet and one are led
Gas tube socket, the soft air-guide pipe other end are socketed with check valve inlet；F. by unidirectional valve air gate and another soft air-guide pipe
Socket, the soft air-guide pipe other end are socketed with collection airbag air inlet pipe, test tube, check valve, collection airbag is connected group by soft air-guide pipe
Into gas collecting apparatus；G. gas collecting apparatus is put into incubator and cultivated, and collect gas；H. received after the completion of gas automatic collection
Collection is in collection airbag.
After this design, the present invention has the advantages that following three：（1）Test tube of the present invention is equipped with scale, easy to quantitative
Dispense fluid nutrient medium；（2）Test tube of the present invention is relatively reliable using being threadedly coupled with seal cap, and centre is sealed using gasket,
Sealing effect is more preferable；（3）The check valve of connection can control gas unidirectional in gas collecting apparatus to flow among soft air-guide pipe, can be effective
Gas is collected, easy to observe and qualitative and quantitative analysis.
Brief description of the drawings
Above-mentioned is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention, below in conjunction with
The present invention is described in further detail with embodiment for attached drawing.
Fig. 1 is the collection device general structure schematic diagram for the gas that a kind of microorganism of the present invention produces.
Fig. 2 is the collection device seal cap sectional view for the gas that a kind of microorganism of the present invention produces.
Fig. 3 is the collection device check valve sectional view for the gas that a kind of microorganism of the present invention produces.
Fig. 4 is the collection device collection airbag schematic diagram for the gas that a kind of microorganism of the present invention produces.
Embodiment
With reference to shown in Fig. 1-4, a kind of collection device of the gas produced the present invention relates to microorganism, including test tube 1, sealing
Cap 2,5 sum aggregate airbag 7 of check valve, wherein：The test tube 1 is equipped with scale, and test tube mouth is equipped with external screw thread；The seal cap 2
1 top of test tube is installed on using being threadedly coupled；2 center of top of seal cap is equipped with an air outlet pipe 8, and seal cap 2 is equipped with interior
Screw thread；Needle 11 and spring 12 are installed in the check valve 5,11 leakproof one-way valve of needle, 5 air inlet is jacked up using spring 12.
Test tube 1 is the main element of the present invention, and pref. cylindrical designs.1 outer wall of test tube is equipped with scale, is quantified using scale
Add fluid nutrient medium.1 mouth of pipe of test tube is equipped with external screw thread, and with seal cap 2 using being threadedly coupled, connecting portion contact position is using close
Packing 3 is sealed.
Seal cap 2 is designed with internal thread, and center of top is equipped with air outlet pipe 8；2 built-in gasket 3 of seal cap is connected with test tube,
Make to seal herein more reliable.
4 one end of soft air-guide pipe is socketed in air outlet pipe 8, and 4 other end of soft air-guide pipe is socketed on the air inlet of check valve 5；
6 one end of soft air-guide pipe is socketed on the gas outlet of check valve 5, and 6 other end of soft air-guide pipe is socketed in the air inlet pipe 9 of collection airbag 7；
Soft air-guide pipe is connected with each inlet, outlet using interference fit, circumferential seal.
Spring 12 is installed in valve deck 14 on cylinder in check valve 5；11 cylindrical section of needle is installed in 12 other end of spring；
Gasket 13 is installed on valve deck 14 and among valve chamber 10, cylindrical used with 10 inner circle of valve chamber of valve deck 14 is interference fitted；Needle 11 is set
There is circular cone guiding, when the disengaging air inlet of ambassador's needle 11 is crossed in air pressure in test tube 1, gas enters in check valve.
Connected between collection airbag 7 and check valve 5 by soft air-guide pipe 6, gas, which is entered in check valve 5, passes through soft air-guide pipe
6 enter in collection airbag 7.
The present invention also provides a kind of application method of above-mentioned gas collection device, sealing ring is attached to corresponding positions before use
Put, then aseptic culture medium is quantified and is poured into graduated test tube 1, it is close after being inoculated with certain microorganism or sample containing microorganism
Sealing cap 2 is connected and screwing hermetic cap 2 with test tube 1, and 2 air outlet pipe 8 of seal cap is socketed with soft air-guide pipe 4,4 other end of soft air-guide pipe and
5 air inlet of check valve is socketed, and 5 gas outlet of check valve is socketed with soft air-guide pipe 6,6 other end of soft air-guide pipe and collection 7 air inlet pipe of airbag
9 sockets, so that each component, using soft air-guide pipe connection composition gas collecting apparatus, gas collecting apparatus is put into incubator and cultivates, automatic to receive
Collect gas, by collecting airbag whether there is swelling observation aerogenesis situation, and carry out qualitative and quantitative analysis.
Many places of the present invention use end face seal, make sealing relatively reliable.Entire body of the present invention uses transparent material, is testing
Easy to observation in journey.Test tube 1 of the present invention uses cylinder design, and test tube bottom is plane, more convenient to use.Structure letter of the present invention
It is single, it is small, it is easy to operate.
The aerogenesis situation that case 1 detects Escherichia coli is embodied：Aseptically nutrient broth medium is loaded on
In the test tube of gas collecting apparatus, after being inoculated with Escherichia coli, tighten seal cap and check the air-tightness of each joint, the dress after inspection
Be placed in constant temperature incubation in 37 DEG C of incubators, when culture 18 is small after observe collection airbag expansion；Without being inoculated with Escherichia coli
Blank control, collection airbag swelling is not observed under the same terms；This experimental result with traditional " the small tube method of Du Shi "
Unanimously.
Specific implementation case 2 detects a kind of aerogenesis situation of lactobacillus acetotolerans：It is aseptically that lactic acid bacteria is special
In test tube of the MRS fluid nutrient mediums loaded on gas collecting apparatus, after being inoculated with this kind of lactobacillus acetotolerans, tighten seal cap and check each connect
Air-tightness at head, the device after inspection are placed in constant temperature incubation in 37 DEG C of incubators, and culture observes that collection airbag is slightly swollen after 7 days
It is swollen；Blank control without being inoculated with this kind of lactobacillus acetotolerans, collection airbag swelling is not observed under the same terms；Together
When, Du Shi tubules, which are also not observed, in the experimental result of traditional " the small tube method of Du Shi " minute bubbles generation, illustrates that the present invention is excellent
In traditional method；The gas collected in airbag is analyzed again, measurement result is shown as carbon dioxide.
Claims (11)
1. a kind of microorganism produces the collection device of gas, it is characterised in that including with graduated test tube, built-in gasket
Seal cap, check valve, soft air-guide pipe, collection airbag, wherein：
The test tube mouth of pipe is equipped with external screw thread, and test tube is in cylinder；
For the seal cap with test tube using being threadedly coupled, a built-in sealing ring, seal cap top is equipped with hard air outlet pipe；
Connected between the hard air outlet pipe and check valve by soft air-guide pipe, the unidirectional stream of check valve control whole device gas
It is dynamic；
The check valve is connected with collection airbag air inlet by soft air-guide pipe.
2. a kind of microorganism according to claim 1 produces the collection device of gas, it is characterised in that：Outside the test tube
Wall is equipped with scale, and for test tube in cylinder, bottom is plane.
3. a kind of microorganism according to claim 1 produces the collection device of gas, it is characterised in that：The test tube pipe
Mouth is equipped with external screw thread, and the seal cap is equipped with internal thread corresponding with test tube external screw thread.
4. a kind of microorganism according to claim 3 produces the collection device of gas, it is characterised in that：The seal cap
If internal thread, for being connected with test tube.
5. a kind of microorganism according to claim 4 produces the collection device of gas, it is characterised in that：The seal cap
One gasket is inside installed.
6. a kind of microorganism according to claim 4 produces the collection device of gas, it is characterised in that：The seal cap
Center of top is equipped with a gas outlet, connects a hard air outlet pipe gas outlet seal, leads between the air outlet pipe and check valve
Cross a soft air-guide pipe socket.
7. a kind of microorganism according to claim 1 produces the collection device of gas, it is characterised in that：The check valve
It is interior to be equipped with spring and needle.
8. a kind of microorganism according to claim 7 produces the collection device of gas, it is characterised in that：The needle is set
There is the circular cone of guiding.
9. a kind of microorganism according to claim 1 produces the collection device of gas, it is characterised in that：The collection airbag
Opening is equipped with rigid plastics air inlet pipe.
10. a kind of microorganism according to claim 6 produces the collection device of gas, it is characterised in that：Described is unidirectional
It is socketed between valve and collection airbag by another soft air-guide pipe.
11. the collection device of gas is produced according to a kind of microorganism described in claim 1, it is characterised in that：The sealing
Cap is provided with gasket with the graduated test tube junction of the band.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720823308.5U CN207330923U (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism produces the collection device of gas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720823308.5U CN207330923U (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism produces the collection device of gas |
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| Publication Number | Publication Date |
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| CN207330923U true CN207330923U (en) | 2018-05-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720823308.5U Active CN207330923U (en) | 2017-07-10 | 2017-07-10 | A kind of microorganism produces the collection device of gas |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207354A (en) * | 2018-11-19 | 2019-01-15 | 农业部规划设计研究院 | A kind of microbial fermentation production potential test device |
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2017
- 2017-07-10 CN CN201720823308.5U patent/CN207330923U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207354A (en) * | 2018-11-19 | 2019-01-15 | 农业部规划设计研究院 | A kind of microbial fermentation production potential test device |
| CN109207354B (en) * | 2018-11-19 | 2023-09-05 | 农业部规划设计研究院 | Microorganism fermentation gas production potential testing device |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201126 Address after: 637400 No. 63 Park Road, Nanchong, Sichuan, Langzhong Patentee after: Sichuan Baoning Vinegar Co.,Ltd. Address before: Ya'an City, Sichuan Province, 625041 new Yucheng Kang Road No. 46 Patentee before: SICHUAN AGRICULTURAL University |