CN209260093U - Parallel cell culture tank - Google Patents
Parallel cell culture tank Download PDFInfo
- Publication number
- CN209260093U CN209260093U CN201821721607.9U CN201821721607U CN209260093U CN 209260093 U CN209260093 U CN 209260093U CN 201821721607 U CN201821721607 U CN 201821721607U CN 209260093 U CN209260093 U CN 209260093U
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- tank body
- cover
- tank
- cell culture
- parallel cell
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Abstract
The utility model belongs to organism culturing device field, is related to a kind of parallel cell culture tank, including tank body, and tank body top is equipped with cover, tank body and cover and is fixed by compressing O-ring mode, agitating paddle is equipped in tank body, agitating paddle spindle top runs through cover；Feed supplement connector and inlet suction port are equipped with outside tank body, feed supplement connector passes through cover by feed supplement branch pipe and is connected to tank interior, and inlet suction port is communicated in cover by external pipeline or the intracorporal respective line of tank；Cover is equipped with sample tap, is placed with sampling plug engaged therewith in sample tap；Cover is equipped with pH electrode interface.The structure design of the utility model is reasonable, and tank body can be used as disposable tank body and use, and is connected and fixed between cover and tank body using compressing ready-package, and cover components itself are less, after culture, culture solution to topple over transfer more convenient；And feed supplement connector and inlet suction port are connected in tank body and cover, feed operation is simpler efficiently.
Description
Technical field
The utility model relates to a kind of parallel cell culture tanks, belong to organism culturing device field.
Background technique
Parallel cell culture systems are one, and that microorganism, human archeocyte, animal and plant cells are carried out under controlled condition is parallel
The system of culture, set of system have several groups fermentation unit, and volume of culture range is 200ml-1000mL, support more set systems
Combination, to have bigger culture flux.The tank body of existing parallel cell culture systems generally uses glass material, is using
Before need to carry out it cleaning and sterilization treatment, cover and tank body are connected using the installation of rotary or fastener formula, and general
In cover top layout compared with multiple component, this mode is primarily present cleaning and is not thorough and sterilizes incomplete risk；In addition,
After parallel cell incubation, need in culture tank reagent take out when, due to cover top layout components compared with
It is more, cause overall weight larger, meanwhile, cover connects complexity with tank body, and disassembly is inconvenient.
Utility model content
The purpose of this utility model is that proposing a kind of parallel cell culture tank, the tank body of the parallel cell culture tank can be made
It for the use of disposable tank body, is connected and fixed between cover and tank body using compression ready-package, cover components itself are less, training
Support after, culture solution topple over transfer it is more convenient；And feed supplement connector and inlet suction port, feed supplement are connected in tank body and cover
It operates simpler efficient.
Parallel cell culture tank described in the utility model, including tank body, tank body top are equipped with cover, and tank body and cover are logical
It crosses compression mode to fix, agitating paddle is equipped in tank body, agitating paddle spindle top runs through cover；Be equipped with outside tank body feed supplement connector and
Inlet suction port, feed supplement connector pass through cover by feed supplement branch pipe and are connected to tank interior, and inlet suction port is connected to by external pipeline
In cover or the intracorporal respective line of tank；Cover is equipped with sample tap, is placed with sampling plug engaged therewith in sample tap；
Cover is equipped with pH electrode interface.
When parallel cell culture, tank body and cover are fixed by compression mode, the outer driving dress of agitating paddle spindle top connection
It sets.Feed supplement connector includes four tubular structures, is respectively connected to by a feed supplement branch pipe across cover at the top of four tubular structures
Tank interior, four bottom part of tubular structure are correspondingly connected with other connectors, and four feed supplement branch pipes supplement acid solution, alkali into tank body respectively
Liquid, culture medium, defoaming agent.In parallel cell incubation, when needing to sample, sampling plug is taken out from sample tap, completes to take
Sampling plug is put back to again after sample and blocks sample tap, sampling plug periphery is equipped with circumferential seal circle, can be more preferable by sample tap
Sealing.PH electrode interface is used to install pH electrode, and the pH value in culture solution in incubation is measured and controlled, according to
Culture process requirement is passed through the amount of carbon dioxide in tank body by control to adjust the pH value of culture solution.
Preferably, the inlet suction port includes three tubular structures, and three tubular structures have been respectively communicated with surface layer air inlet
One end of hose, tail gas condensing hose and bottom air induction hose, surface layer air induction hose, tail gas condensing hose and bottom air induction hose
The other end be respectively communicated with the surface layer air inlet pipe in cover, the coiled coil hose in cover and be connected to tank body bottom
Gas-distributing pipe.Surface layer air induction hose is communicated in the air inlet that the surface layer air inlet pipe in cover realizes surface layer；Tail gas condensing hose connects
The logical coiled coil hose in cover according to the technological requirements, can be passed through frequently in parallel cell incubation in tank body
Air, oxygen or carbon dioxide, at this point, pressure can increase in tank body, in order to keep the stabilization of pressure inside the tank, configuration exhaust
Pipe, i.e. tail gas condensing pipe, meanwhile, moisture is taken away when being vented in order to prevent, by cooling to cover, by spiral shell in cover
It is cooling to revolve Coiled hose, so that the condensate moisture in hose, comes back in tank body；Bottom air induction hose is connected to tank body bottom
Gas-distributing pipe, air, oxygen, carbon dioxide can be distributed to tank base by gas-distributing pipe, be used for microorganism or zooblast
Culture medium.
Preferably, the medium position of bottom is equipped with annular groove in the cover, is in around main shaft at the top of agitating paddle
Umbrella-shaped structure is equipped with umbrella ring, and umbrella ring is in annular groove.Umbrella ring realizes agitating paddle fixed in annular groove
Position, to guarantee the stable working state of agitating paddle；And annular groove may further be used to realize the winding of coiled coil hose, protect
It is more firm to demonstrate,prove coiled coil hose position.
Preferably, the tank base is equipped with the hollow protrusion of cone cell, and the hollow protrusion of cone cell is raised to tank interior.Cone cell
Whole positioning of the hollow protrusion for tank body, guarantees the positional accuracy of tank body, tank body position it is accurate, it is ensured that tank body bottom
Micro- lambda sensor patch is corresponding with the position of sensor, accurate at portion's arc groove.
Preferably, the tank interior bottom surface is equipped with locating shaft, and locating shaft is embedded in the location hole of agitating paddle main shaft bottom end
It is interior, guarantee that agitating paddle operation is more steady；And the main shaft lower part of agitating paddle is equipped with double-layer paddle, increases the uniform of stirring
Property.
Preferably, bottom surface is equipped with arc groove outside the tank body, for micro- lambda sensor patch to be arranged.
Preferably, the tank body is in gradually smaller cone-shaped from top to bottom, and tank interior is circumferentially evenly equipped with folding
Flowing plate.
Preferably, O-ring seal is equipped between the tank body and cover.Tank end is inserted in O-ring seal, then will
Cover is pressed into from top of the tank, and under the action of O-ring seal, cover and tank body realize that ready-package is tightly connected, the two disassembly
It is more convenient.
Preferably, the tank body, cover, agitating paddle, feed supplement connector, inlet suction port and sampling plug are PC material system
At.Using PC material, be molded, cost is lower, have disposable characteristic, eliminate due to sterilizing be not thorough and
Using the preceding risk polluted again, and gas-distributing pipe etc. is that PC material is made.
Possessed beneficial effect is the utility model compared with prior art:
The structure design of the utility model is reasonable, and tank body, cover etc. are made of the molding of PC material molds, and cost is lower, tool
Once property uses characteristic, eliminates since sterilizing is not thorough and using the preceding risk polluted again；Between cover and tank body
Using compress ready-package be connected and fixed, cover components itself are less, after culture, culture solution topple over transfer it is more square
Just；And feed supplement connector and inlet suction port are connected in tank body and cover, feed operation is simpler efficiently.
Detailed description of the invention
Fig. 1, parallel cell culture tank structural schematic diagram (main view)；
Fig. 2, A-A cross-sectional view；
Fig. 3, parallel cell culture tank structural schematic diagram (top view).
In figure: 1, tank body；2, plug is sampled；3, feed supplement branch pipe；4, cover；5, inlet suction port；6, feed supplement connector；7, it stirs
Paddle；8, baffle plate；9, the hollow protrusion of cone cell；10, locating shaft；11, arc groove；12, gas-distributing pipe；13, umbrella ring；14, annular
Groove；15, pH electrode interface；16, surface layer air induction hose；17, tail gas condensing hose；18, bottom air induction hose；19, O-shaped sealing
Circle.
Specific embodiment
The utility model is described further with reference to the accompanying drawing:
Such as Fig. 1-Fig. 3, parallel cell culture tank described in the utility model, including tank body 1,1 top of tank body are equipped with cover
4, tank body 1 is fixed with cover 4 by compression mode, and agitating paddle 7 is equipped in tank body 1, and 7 spindle top of agitating paddle runs through cover 4；Tank
Feed supplement connector 6 and inlet suction port 5 are equipped with outside body 1, feed supplement connector 6 passes through cover 4 by feed supplement branch pipe 3 and is connected in tank body 1
Portion, inlet suction port 5 are communicated in the respective line in cover 4 or in tank body 1 by external pipeline；Cover 4 is equipped with sample tap, takes
Sampling plug 2 engaged therewith is placed in sample mouth；Cover 4 is equipped with pH electrode interface 15.
In the present embodiment:
Inlet suction port 5 includes three tubular structures, and it is cold that three tubular structures have been respectively communicated with surface layer air induction hose 16, tail gas
One end of solidifying hose 17 and bottom air induction hose 18, surface layer air induction hose 16, tail gas condensing hose 17 and bottom air induction hose 18
The other end be respectively communicated with the surface layer air inlet pipe in cover 4, the coiled coil hose in cover 4 and be connected to tank body 1
The gas-distributing pipe 12 of bottom.Surface layer air induction hose 16 is communicated in the air inlet that the surface layer air inlet pipe in cover 4 realizes surface layer；Tail gas is cold
Solidifying hose 17 is communicated in the coiled coil hose in cover 4, in parallel cell incubation, according to the technological requirements, tank body 1
It is interior to be passed through air, oxygen or carbon dioxide frequently, at this point, pressure can increase in tank body 1, in order to keep the steady of pressure inside the tank
It is fixed, exhaust pipe, i.e. tail gas condensing pipe are configured, meanwhile, moisture is taken away when being vented in order to prevent, by being dropped to the cooling of cover 4
Temperature, 4 inside spin Coiled hose of cover is cooling, so that the condensate moisture in hose, comes back in tank body 1；Bottom air inlet is soft
Pipe 18 is connected to the gas-distributing pipe 12 of 1 bottom of tank body, and air, oxygen, carbon dioxide can be distributed to 1 bottom of tank body by gas-distributing pipe 12
Portion, for microorganism or the culture medium of zooblast.
The medium position of bottom is equipped with annular groove 14 in cover 4, sets around 7 top main shaft of agitating paddle in umbrella-shaped structure
There is umbrella ring 13, umbrella ring 13 is in annular groove 14.Umbrella ring 13 realizes agitating paddle 7 fixed in annular groove 14
Position, to guarantee the stable working state of agitating paddle 7；And annular groove 14 may further be used to realize twining for coiled coil hose
Around guarantee coiled coil hose position is more firm.
1 bottom of tank body is equipped with cone cell hollow raised 9, and cone cell hollow raised 9 is to 1 internal protrusion of tank body.Cone cell hollow raised 9
For tank body 1 entirety positioning, guarantee tank body 1 positional accuracy, 1 position of tank body it is accurate, it is ensured that 1 bottom arc of tank body
Micro- lambda sensor patch is corresponding with the position of sensor, accurate at connected in star 11.
1 inner bottom surface of tank body is equipped with locating shaft 10, and locating shaft 10 is embedded in the positioning hole of 7 main shaft bottom end of agitating paddle, guarantees
The operation of agitating paddle 7 is more steady；And the main shaft lower part of agitating paddle 7 is equipped with double-layer paddle, increases the uniformity of stirring.
1 outside bottom surface of tank body is equipped with arc groove 11, for micro- lambda sensor patch to be arranged.
Tank body 1 is in gradually smaller cone-shaped from top to bottom, and 1 internal circumferential of tank body is evenly equipped with baffle plate 8.
O-ring seal 19 is equipped between tank body 1 and cover 4.O-ring seal 19 is inserted in 1 end of tank body, then by cover 4
It is pressed at the top of tank body 1, under the action of O-ring seal 19, cover 4 and tank body 1 realize that ready-package is tightly connected, the two disassembly
It is more convenient.
Tank body 1, cover 4, agitating paddle 7, feed supplement connector 6, inlet suction port 5 and sampling plug 2 are that PC material is made.Using
PC material, is molded, and cost is lower, has disposable characteristic, before eliminating since sterilizing is not thorough and use
The risk polluted again, and gas-distributing pipe 12 etc. is that PC material is made.
When parallel cell culture, O-ring seal 19 is inserted in 1 end of tank body, is then pressed into cover 4 at the top of tank body 1,
Under the action of O-ring seal 19, cover 4 and tank body 1 realize that fast-assembling compression type is tightly connected, and the connection of 7 spindle top of agitating paddle is outer
Driving device.Feed supplement connector 6 includes four tubular structures, respectively passes through tank by a feed supplement branch pipe 3 at the top of four tubular structures
Lid 4 is connected to inside tank body 1, and bottom is correspondingly connected with other connectors, four feed supplement branch pipes 3 supplemented respectively into tank body 1 acid solution,
Lye, culture medium, defoaming agent；Surface layer air induction hose 16 is communicated in the air inlet that the surface layer air inlet pipe in cover 4 realizes surface layer；Tail
The solidifying hose 17 that is gas-cooled is communicated in the coiled coil hose in cover 4, in parallel cell incubation, according to the technological requirements,
Air, oxygen or carbon dioxide can be passed through in tank body 1 frequently, at this point, pressure can increase in tank body, in order to keep pressure inside the tank
Stabilization, configure exhaust pipe, i.e. tail gas condensing pipe, meanwhile, moisture is taken away when being vented in order to prevent, by the cold of cover 4
But cool down, 4 inside spin Coiled hose of cover is cooling, so that the condensate moisture in hose, comes back in tank body 1；Bottom into
Gas hose 18 is connected to the gas-distributing pipe 12 of 1 bottom of tank body, and air, oxygen, carbon dioxide can be distributed to tank by gas-distributing pipe 12
1 bottom of body, for microorganism or the culture medium of zooblast.It is when needing to sample, sampling is stifled in parallel cell incubation
First 2 take out from sample tap, complete again to put back to sampling plug 2 after sampling and block sample tap, sampling 2 periphery of plug is equipped with circumferential
Sealing ring, can be by sample tap better seal.PH electrode interface 15 is for installing pH electrode, in culture solution in incubation
PH value measure and control, required according to culture process, be passed through the amount of carbon dioxide in tank body 1 by controlling and adjust training
The pH value of nutrient solution.
Claims (9)
1. a kind of parallel cell culture tank, it is characterised in that: including tank body (1), tank body (1) top is equipped with cover (4), tank body
(1) it is fixed with cover (4) by compression mode, is equipped with agitating paddle (7) in tank body (1), agitating paddle (7) spindle top runs through cover
(4)；Feed supplement connector (6) and inlet suction port (5) are equipped with outside tank body (1), feed supplement connector (6) passes through tank by feed supplement branch pipe (3)
Lid (4) is connected to tank body (1) inside, and inlet suction port (5) is communicated in cover (4) or in tank body (1) by external pipeline
Respective line；Cover (4) is equipped with sample tap, is placed with sampling plug (2) engaged therewith in sample tap；Cover (4) is equipped with pH
Electrode interface (15).
2. parallel cell culture tank according to claim 1, it is characterised in that: inlet suction port (5) includes three tubular knots
Structure, three tubular structures have been respectively communicated with surface layer air induction hose (16), tail gas condensing hose (17) and bottom air induction hose (18)
One end, the other end of surface layer air induction hose (16), tail gas condensing hose (17) and bottom air induction hose (18), which is respectively communicated with, to be set
In the surface layer air inlet pipe in cover (4), the coiled coil hose in cover (4) and the gas-distributing pipe for being connected to tank body (1) bottom
(12)。
3. parallel cell culture tank according to claim 1, it is characterised in that: the medium position of cover (4) interior bottom is set
Have annular groove (14), be equipped with umbrella ring (13) in umbrella-shaped structure around main shaft at the top of agitating paddle (7), umbrella ring (13) is embedded in
In annular groove (14).
4. parallel cell culture tank according to claim 1, it is characterised in that: it is hollow convex that tank body (1) bottom is equipped with cone cell
It rises (9), cone cell hollow raised (9) is to tank body (1) internal protrusion.
5. parallel cell culture tank according to claim 1, it is characterised in that: tank body (1) inner bottom surface is equipped with locating shaft
(10), locating shaft (10) is embedded in the positioning hole of agitating paddle (7) main shaft bottom end.
6. parallel cell culture tank according to claim 1, it is characterised in that: it is recessed that the external bottom surface of tank body (1) is equipped with arc
Slot (11).
7. parallel cell culture tank according to claim 1, it is characterised in that: tank body (1) is in gradually become smaller from top to bottom
Cone-shaped, and tank body (1) internal circumferential is evenly equipped with baffle plate (8).
8. parallel cell culture tank according to claim 1, it is characterised in that: be equipped with O between tank body (1) and cover (4)
Type sealing ring (19).
9. parallel cell culture tank described in any one of -8 according to claim 1, it is characterised in that: tank body (1), cover
(4), agitating paddle (7), feed supplement connector (6), inlet suction port (5) and sampling plug (2) are that PC material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821721607.9U CN209260093U (en) | 2018-10-23 | 2018-10-23 | Parallel cell culture tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821721607.9U CN209260093U (en) | 2018-10-23 | 2018-10-23 | Parallel cell culture tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209260093U true CN209260093U (en) | 2019-08-16 |
Family
ID=67558799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821721607.9U Active CN209260093U (en) | 2018-10-23 | 2018-10-23 | Parallel cell culture tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209260093U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772296A (en) * | 2021-03-09 | 2021-05-11 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| US11872562B1 (en) * | 2021-03-29 | 2024-01-16 | The United States Of America As Represented By The Secretary Of The Army | Chemical detection training container and method for use thereof |
-
2018
- 2018-10-23 CN CN201821721607.9U patent/CN209260093U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772296A (en) * | 2021-03-09 | 2021-05-11 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| CN112772296B (en) * | 2021-03-09 | 2023-07-04 | 贵州贵旺生物科技有限公司 | Liquid strain culture device and culture method for pleurotus eryngii |
| US11872562B1 (en) * | 2021-03-29 | 2024-01-16 | The United States Of America As Represented By The Secretary Of The Army | Chemical detection training container and method for use thereof |
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