CN206591137U - A kind of cell culture apparatus - Google Patents
A kind of cell culture apparatus Download PDFInfo
- Publication number
- CN206591137U CN206591137U CN201720252959.3U CN201720252959U CN206591137U CN 206591137 U CN206591137 U CN 206591137U CN 201720252959 U CN201720252959 U CN 201720252959U CN 206591137 U CN206591137 U CN 206591137U
- Authority
- CN
- China
- Prior art keywords
- bottle
- cell culture
- culture apparatus
- cell
- piping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a kind of cell culture apparatus, be related to technical field of cell culture, for solve artificial piping and druming cell it is inaccurate to Stress control, it is uneven the problems such as design.Cell culture apparatus includes blake bottle and piping and druming device, the blake bottle includes outer blank and the interior bottle being rotatably arranged in the outer blank, the piping and druming device includes airway tube and the inflating pump being connected with the airway tube, the end that the airway tube is stretched into the interior bottle is connected with piping and druming pin or suction pipe, and the inflating pump is connected with controller.It can control to blow pressure of the big device to cell by controller, and rotated by interior bottle, the cell in the internal bottle of piping and druming device can be made comprehensively to be blown and beaten, cell mass is avoided to assemble, the Stress control for blowing and beating cell is accurate, uniform, influence of the manually-operated error to cytoactive is avoided, time and artificial energy is saved.
Description
Technical field
The utility model is related to technical field of cell culture, more particularly to a kind of cell culture apparatus.
Background technology
Cell culture technology is also cell clone technology, is most crucial, most basic technology in biotechnology, most
Biological product is typically all what is got by cell culture, and cell culture has been widely used in biology, medical science, new drug development
Etc. every field, as one of most important basic science.No matter for whole biotechnology, or the life of one of them
For thing clone technology, cell culture is all an essential process.Cell culture technology can be passed through by a cell
Mass propgation turns into many cells that are simple unicellular or seldom breaking up, is a kind of extensive clone technology of cell.
, it is necessary to control the temperature of blake bottle or culture dish according to different situations in the incubation of cell, and need
Cell is blown and beaten to eliminate the aggregation of cell mass.In the prior art, testing crew operation piping and druming pin, suction pipe or centrifugation are generally required
Pipe etc. carries out pressing piping and druming to cell, had not only expended the time but also had expended energy, and manually-operated accuracy is poor, in operating process
Control to pressure is poor, during piping and druming it is uneven on the pressure of cell, excessive or it is too small can all influence the activity of cell so that
Influence final cultivation results.
Utility model content
The purpose of this utility model is to propose a kind of cell culture apparatus, and the device can control blake bottle to blow and beat automatically
Cell, the Stress control for blowing and beating cell is accurate, uniform, it is to avoid influence of the manually-operated error to cytoactive, saves the time
And artificial energy.
For up to this purpose, the utility model uses following technical scheme：
A kind of cell culture apparatus, including blake bottle and piping and druming device, the blake bottle include outer blank and rotatable set
The interior bottle being placed in the outer blank, the piping and druming device includes airway tube and the inflating pump being connected with the airway tube, described
The end that airway tube is stretched into the interior bottle is connected with piping and druming pin or suction pipe, and the inflating pump is connected with controller.
Wherein, the suction pipe is elbow straw.
Wherein, the angle of the bearing of trend of the end of the elbow straw and the elbow straw is 30 ° -60 °.
Wherein, the angle of the bearing of trend of the end of the elbow straw and the elbow straw is 45 °.
Wherein, the cell culture apparatus also includes rotational motion mechanism, the output shaft of the rotational motion mechanism and institute
The bottom for stating interior bottle is fixed.
Wherein, be provided with heater between the interior bottle and the outer blank, the heater respectively with the control
Device and the temperature sensor electrical connection being arranged on the inwall of the interior bottle.
Wherein, the heater includes the resistance wire for winding the outer wall of the interior bottle.
Wherein, the outer blank is made up of insulation material.
Wherein, it is provided with the blake bottle on bottle cap, the bottle cap and is provided with through hole, the airway tube passes through described logical
Hole is stretched into the interior bottle.
Wherein, it is provided with charging aperture on the bottle cap.
Beneficial effect：The utility model provides a kind of cell culture apparatus, including blake bottle and piping and druming device, the training
Supporting bottle includes outer blank and the interior bottle that is rotatably arranged in the outer blank, the piping and druming device include airway tube and with it is described
The inflating pump of airway tube connection, the end that the airway tube is stretched into the interior bottle is connected with piping and druming pin or suction pipe, described to inflate
Pump is connected with controller.It can control to blow and beat pressure of the device to cell by controller, and be rotated by interior bottle, can make to blow
The cell beaten in the internal bottle of device is comprehensively blown and beaten, it is to avoid cell aggregation, and the Stress control for blowing and beating cell is accurate, uniform,
Influence of the manually-operated error to cytoactive is avoided, time and artificial energy is saved.
Brief description of the drawings
Fig. 1 is the structural representation for the cell culture apparatus that the utility model tool is provided.
Wherein：
1st, blake bottle；11st, outer blank；12nd, interior bottle；21st, airway tube；22nd, suction pipe；3rd, rotational motion mechanism；4th, heater；
5th, bottle cap；51st, through hole；52nd, charging aperture.
Embodiment
To make the utility model technical problem solved, the technical scheme used and the technique effect reached clearer,
Further illustrate the technical solution of the utility model below in conjunction with the accompanying drawings and by embodiment.
The utility model provides a kind of cell culture apparatus, including blake bottle 1 and piping and druming device, and blake bottle 1 includes outer
Bottle 11 and the interior bottle 12 that is rotatably arranged in outer blank 11, piping and druming device include airway tube 21 and are connected with airway tube 21
Inflating pump, the end that airway tube 21 is stretched into interior bottle 12 is connected with piping and druming pin or suction pipe 22, and inflating pump is connected with controller.
In use, controlling the pressure of the output gas of inflating pump by controller so that there is piping and druming pin or suction pipe 22 to blow out
Gas pressure it is constant, meanwhile, interior bottle 12 rotates so that piping and druming pin or suction pipe 22 blow out gas can uniformly blow and beat interior bottle
Culture cell in 12 everywhere, prevents the cell aggregation in interior bottle 12 into cell mass, so as to influence the activity of cell.Relative to existing
There is artificial operation piping and druming cell in technology, the cell culture apparatus that the utility model is provided can blow and beat cell automatically, and piping and druming is thin
The Stress control of born of the same parents is accurate, uniform, it is to avoid influence of the manually-operated error to cytoactive, saves time and artificial energy.
Specifically, it is provided with blake bottle 1 on bottle cap 5, bottle cap 5 and is provided with through hole 51 and charging aperture 52, airway tube 21
Stretched into by through hole 51 in interior bottle 12.The end that airway tube 21 is stretched into interior bottle 12 can connect piping and druming pin, can also connect suction
Pipe 22, the pressure for blowing and beating pin is larger, and the piping and druming effect of two suction pipes 22 is softer, can be selected as the case may be when actual use
Appropriate device.When the end connected sucking pipe 22 of airway tube 21, suction pipe 22 can be elbow straw, and elbow straw is to cell
Blow and beat effect preferably, be not likely to produce bubble, and when elbow straw end and elbow straw bearing of trend angle be 30 °-
At 60 °, piping and druming effect is relatively good, wherein, the blowing when angle of the end of elbow straw and the bearing of trend of elbow straw is 45 °
Beat better.
In order to uniformly blow and beat the cell in interior bottle 12 everywhere, interior bottle 12 can be under the drive of rotational motion mechanism 3
Rotation, the interior rotation of bottle 12 can also promote the mixing of the interior inner cell of bottle 12 and nutrient solution.Rotational motion mechanism 3 can be that gear is passed
Motivation structure or worm-and-wheel gear etc., the bottom of the output shaft of rotational motion mechanism 3 and interior bottle 12 is fixed, so as to drive interior bottle
12 rotations.
It is stricter to the temperature requirement of blake bottle 1 in the incubation of cell, interior bottle 12 in the utility model with it is outer
Be additionally provided with heater 4 between bottle 11, heater 4 respectively with controller and the temperature being arranged on the inwall of interior bottle 12
Sensor is electrically connected, it is possible to achieve the thermostatic control of culture apparatus.Specifically, heater 4 can include being wrapped in interior bottle
The resistance wire of 12 outer wall, passes through bottle 12 in resistance wire electrified regulation.Outer blank 11 can also be made up of insulation material, so as to enter one
Step realizes the thermostatic control to blake bottle 1.
Above content is only preferred embodiment of the present utility model, for one of ordinary skill in the art, according to this reality
With new thought, it will change in specific embodiments and applications, this specification content should not be construed as
To limitation of the present utility model.
Claims (10)
1. a kind of cell culture apparatus, it is characterised in that including blake bottle (1) and piping and druming device, the blake bottle (1) includes outer
Bottle (11) and the interior bottle (12) that is rotatably arranged in the outer blank (11), the piping and druming device including airway tube (21) and
The inflating pump being connected with the airway tube (21), the end that the airway tube (21) is stretched into the interior bottle (12) is connected with piping and druming
Pin or suction pipe (22), the inflating pump are connected with controller.
2. cell culture apparatus as claimed in claim 1, it is characterised in that the suction pipe (22) is elbow straw.
3. cell culture apparatus as claimed in claim 2, it is characterised in that inhaled with the elbow end of the elbow straw
The angle of the bearing of trend of pipe is 30 ° -60 °.
4. cell culture apparatus as claimed in claim 3, it is characterised in that inhaled with the elbow end of the elbow straw
The angle of the bearing of trend of pipe is 45 °.
5. the cell culture apparatus as described in claim any one of 1-4, it is characterised in that the cell culture apparatus also includes
Rotational motion mechanism (3), the output shaft of the rotational motion mechanism (3) and the bottom of the interior bottle (12) are fixed.
6. the cell culture apparatus as described in claim any one of 1-4, it is characterised in that the interior bottle (12) and the outer blank
(11) it is provided with heater (4) between, the heater (4) is respectively with the controller and being arranged at the interior bottle
(12) the temperature sensor electrical connection on inwall.
7. cell culture apparatus as claimed in claim 6, it is characterised in that the heater (4) is described including being wrapped in
The resistance wire of the outer wall of interior bottle (12).
8. cell culture apparatus as claimed in claim 6, it is characterised in that the outer blank (11) is made up of insulation material.
9. the cell culture apparatus as described in claim any one of 1-4, it is characterised in that be provided with the blake bottle (1)
Through hole (51) is provided with bottle cap (5), the bottle cap (5), the airway tube (21) is stretched into described by the through hole (51)
In bottle (12).
10. cell culture apparatus as claimed in claim 9, it is characterised in that be provided with charging aperture on the bottle cap (5)
(52)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720252959.3U CN206591137U (en) | 2017-03-15 | 2017-03-15 | A kind of cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720252959.3U CN206591137U (en) | 2017-03-15 | 2017-03-15 | A kind of cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206591137U true CN206591137U (en) | 2017-10-27 |
Family
ID=60124160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720252959.3U Expired - Fee Related CN206591137U (en) | 2017-03-15 | 2017-03-15 | A kind of cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206591137U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109554297A (en) * | 2018-12-12 | 2019-04-02 | 广州市华南农大生物药品有限公司 | A kind of cell dispels device and the cell secondary culture method based on it |
-
2017
- 2017-03-15 CN CN201720252959.3U patent/CN206591137U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109554297A (en) * | 2018-12-12 | 2019-04-02 | 广州市华南农大生物药品有限公司 | A kind of cell dispels device and the cell secondary culture method based on it |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171027 Termination date: 20200315 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |