CN112772296B - Liquid strain culture device and culture method for pleurotus eryngii - Google Patents
Liquid strain culture device and culture method for pleurotus eryngii Download PDFInfo
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- CN112772296B CN112772296B CN202110252665.1A CN202110252665A CN112772296B CN 112772296 B CN112772296 B CN 112772296B CN 202110252665 A CN202110252665 A CN 202110252665A CN 112772296 B CN112772296 B CN 112772296B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Abstract
A liquid strain culture device for Pleurotus eryngii comprises a culture tank and a PH measurement assembly. The PH measuring assembly comprises a plurality of PH measuring electrodes and probes, the PH measuring electrodes are arranged in the probes, and notches for exposing the PH measuring electrodes are formed in the side walls of the probes. The probe is arranged along the diameter direction of the culture tank and is close to the tank bottom of the culture tank, the probe penetrates through the side wall of the culture tank and is in sliding fit with the side wall of the culture tank, the probe is in sliding seal with the side wall of the culture tank, and the probe is driven by the driving assembly. The probe has first slip dead point and second slip dead point, and the breach is located inside the culture tank when the probe is located first slip dead point, and the breach is located the culture tank outside when the probe is located the second slip dead point. The method can accurately measure the pH value of the culture system, has higher sensitivity and accuracy, and is favorable for accurately controlling the pH value of the culture system. The culture method is convenient to operate, and the PH value of the culture system can be accurately measured.
Description
Technical Field
The invention relates to the field of liquid strain culture, in particular to a liquid strain culture device and a liquid strain culture method for pleurotus eryngii.
Background
When liquid strain culture is carried out, parameters such as temperature, PH value, pressure and the like of a culture system need to be monitored, and the accurate control of the parameters directly relates to the efficiency and effect of strain culture. However, in the conventional equipment, errors often occur in the measurement of the pH value in the process of culturing liquid strains, and the sensitivity is not high enough, so that the accurate control of the pH value of a culture system is not facilitated.
In view of this, the present application is specifically proposed.
Disclosure of Invention
The first object of the invention is to provide a liquid strain culture device for pleurotus eryngii, which has simple structure and convenient use, can accurately measure the pH value of a culture system, has higher sensitivity and accuracy, and is beneficial to accurately controlling the pH value of the culture system.
The second object of the present invention is to provide a culture method which is convenient to operate, can accurately measure the pH value of a culture system, has higher sensitivity and accuracy, and is favorable for accurately controlling the pH value of the culture system.
Embodiments of the present invention are implemented as follows:
a liquid spawn culture device for pleurotus eryngii, comprising: a culture tank and a PH measurement assembly.
The PH measuring assembly comprises a plurality of PH measuring electrodes and probes, the PH measuring electrodes are arranged in the probes, and notches for exposing the PH measuring electrodes are formed in the side walls of the probes. The probe is arranged along the diameter direction of the culture tank and is close to the tank bottom of the culture tank, the probe penetrates through the side wall of the culture tank and is in sliding fit with the side wall of the culture tank, the probe is in sliding seal with the side wall of the culture tank, and the probe is driven by the driving assembly.
The probe has first slip dead point and second slip dead point, and the breach is located inside the culture tank when the probe is located first slip dead point, and the breach is located the culture tank outside when the probe is located the second slip dead point.
Further, a plurality of probes are uniformly spaced along the circumference of the culture tank.
Further, the outside of the culture tank is provided with a sleeve, and the sleeve is covered on the side wall of the culture tank. The outer end of the probe is coaxially connected with a guide rod, and the guide rod penetrates through the end wall of the sleeve and is in sliding fit with the end wall of the sleeve. The guide rod has external threads and is threadedly engaged with a rotating sleeve rotatably engaged with the end wall of the sleeve. The driving assembly is arranged on the end wall of the sleeve, and the power output part of the driving assembly is in transmission connection with the rotating sleeve.
Further, the inner wall of one side of the sleeve, which is close to the bottom of the culture tank, is provided with a water spray pipe and a gas blowing pipe, and when the probe is positioned at the second sliding dead point, the water spray pipe and the gas blowing pipe are both arranged towards the notch.
Further, the inner wall of one side of the sleeve, which is close to the bottom of the culture tank, is provided with a concave area, and the concave area is formed by concave inner wall of the sleeve. The concave area is filled with sponge, and the bottom of concave area is provided with the drain pipe, and the drain pipe has the joint that is used for being connected with negative pressure device.
Further, the bottom of the concave area is provided with diversion trenches, the diversion trenches are formed by the bottom of the concave area in a concave mode, and the diversion trenches are radially distributed along the circumference of the drain pipe.
Further, be provided with seal structure between the lateral wall of probe and culture tank, seal structure includes first sealing washer and second sealing washer. The first sealing ring and the second sealing ring are fixedly connected to the side wall of the culture tank, the two first sealing rings are respectively arranged on two sides of the second sealing ring, and the first sealing ring and the second sealing ring are attached to the outer wall of the probe. The second seal ring has an inner cavity and an inflation conduit for inflating the inner cavity.
Further, along the diameter direction of the second sealing ring, the inner diameter of the inner cavity is one tenth to one eighth of the thickness of the second sealing ring.
Further, the inside and the outside of the side wall of the culture tank are both provided with stop blocks for preventing the first sealing ring from falling off, and the end part of the first sealing ring is attached to the end part of the second sealing ring.
A culture method using the liquid strain culture device comprises the following steps: and driving the probe from the second sliding dead point to the first sliding dead point by using the driving assembly, and measuring the PH value. After the measurement is finished, the probe is driven to a second sliding dead point from a first sliding dead point by using the driving assembly, water is sprayed into the notch by using the water spraying pipe to clean, and then the notch is blow-dried by using the air blowing pipe.
The embodiment of the invention has the beneficial effects that:
in the use process of the liquid strain culture device for the pleurotus eryngii provided by the embodiment of the invention, in order to prolong the service life of the PH measuring electrode and reduce the damage of the culture system to the sensitivity of the PH measuring electrode, when the PH value is not measured, the driving component is used for controlling the probe to be at the second sliding dead point, so that the PH measuring electrode is positioned outside the culture tank and is not immersed in the culture system, and the liquid strain culture device has positive significance for prolonging the service life of the PH measuring electrode and ensuring the sensitivity of the PH measuring electrode.
When the PH value needs to be measured, the probe is driven to the first sliding dead point by the second sliding dead point by the driving component, the probe stretches into the culture tank, the notch enters the culture tank from the outside of the culture tank, and the PH measuring electrode is in contact with the culture system to measure the PH value. After the measurement is finished, the probe is driven to the second sliding dead point from the first sliding dead point by the driving component, and then the probe can be pulled out of the culture tank.
In general, the liquid strain culture device for the pleurotus eryngii, provided by the embodiment of the invention, has the advantages of simple structure and convenience in use, can accurately measure the pH value of a culture system, has higher sensitivity and accuracy, and is beneficial to accurately controlling the pH value of the culture system. The culture method provided by the embodiment of the invention is convenient to operate, can accurately measure the pH value of the culture system, has higher sensitivity and accuracy, and is favorable for accurately controlling the pH value of the culture system.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a liquid spawn culture device according to an embodiment of the present invention when a probe is located at a second sliding dead point;
FIG. 2 is a schematic diagram of a liquid spawn culture device according to an embodiment of the present invention when a probe is located at a first sliding dead point;
FIG. 3 is a schematic diagram of the structure of a probe;
FIG. 4 is a schematic structural view of a sleeve of a liquid spawn culture device according to an embodiment of the present invention;
FIG. 5 is a schematic view of a sleeve of a liquid spawn culture device according to an embodiment of the present invention;
fig. 6 is an enlarged view of area a in fig. 1.
Icon: a liquid spawn culture device 1000; a culture tank 100; a sleeve 110; a recessed region 111; a sponge 112; a drain pipe 113; a diversion trench 114; a water jet pipe 120; a blowing pipe 130; a stopper 140; a PH measurement electrode 200; a probe 300; notch 310; a guide bar 400; rotating sleeve 500; a drive assembly 600; a first seal ring 700; a second seal ring 800; a lumen 810; inflation conduit 820.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "parallel," "perpendicular," and the like, do not denote that the components are required to be absolutely parallel or perpendicular, but may be slightly inclined. For example, "parallel" merely means that the directions are more parallel than "perpendicular" and does not mean that the structures must be perfectly parallel, but may be slightly tilted.
Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
The terms "substantially," "essentially," and the like are intended to be interpreted as referring to the fact that the term is not necessarily to be construed as requiring absolute accuracy, but rather as a deviation. For example: the term "substantially equal to" does not merely mean absolute equality, but is difficult to achieve in actual production and operation, and generally has a certain deviation. Thus, in addition to absolute equality, "approximately equal to" includes the above-described case where there is a certain deviation. In other cases, the terms "substantially", "essentially" and the like are used in a similar manner to those described above unless otherwise indicated.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Examples
Referring to fig. 1, 2 and 3, the present embodiment provides a liquid spawn culture device 1000 for pleurotus eryngii, where the liquid spawn culture device 1000 includes: a culture tank 100 and a PH measuring assembly.
The PH measuring assembly comprises a plurality of PH measuring electrodes 200 and a probe 300, wherein the PH measuring electrodes 200 are arranged in the probe 300, and a notch 310 for exposing the PH measuring electrodes 200 is formed on the side wall of the probe 300. The probe 300 is disposed along the diameter direction of the culture tank 100 and is disposed near the bottom of the culture tank 100, the probe 300 penetrates through the side wall of the culture tank 100 and is in sliding fit with the side wall of the culture tank 100, the probe 300 is in sliding seal with the side wall of the culture tank 100, leakage of the culture solution is avoided, and the probe 300 is driven by the driving assembly 600.
The probe 300 has a first sliding dead point and a second sliding dead point, the notch 310 is located inside the culture tank 100 when the probe 300 is located at the first sliding dead point, and the notch 310 is located outside the culture tank 100 when the probe 300 is located at the second sliding dead point.
In order to prolong the service life of the PH measuring electrode 200 and reduce the damage of the culture system to the sensitivity of the PH measuring electrode 200 during the use, when the PH value is not measured, the driving assembly 600 is used for controlling the probe 300 to be positioned at the second sliding dead point, so that the PH measuring electrode 200 is positioned outside the culture tank 100 and is not immersed in the culture system, and the method has positive significance for prolonging the service life of the PH measuring electrode 200 and ensuring the sensitivity of the PH measuring electrode 200.
When the PH is required to be measured, the probe 300 is driven from the second sliding dead point to the first sliding dead point by the driving assembly 600, the probe 300 extends into the culture tank 100, the notch 310 enters the culture tank 100 from the outside of the culture tank 100, and the PH measuring electrode 200 contacts with the culture system to measure the PH. After the measurement is completed, the probe 300 is driven from the first sliding dead point to the second sliding dead point by the driving assembly 600, and the probe 300 can be pulled out from the culture tank 100.
In general, the liquid strain culture device 1000 has a simple structure and convenient use, can accurately measure the pH value of a culture system, has higher sensitivity and accuracy, and is favorable for accurately controlling the pH value of the culture system.
Further, in this embodiment, a plurality of probes 300 are spaced along the perimeter Xiang Junyun of the culture tank 100 for measuring the pH of various portions of the culture tank 100 to reduce interference and errors due to local variations.
Further, referring to fig. 4 and 5, a sleeve 110 is disposed on the outer side of the culture tank 100, and the sleeve 110 is covered on the side wall of the culture tank 100 and fixedly connected to the side wall of the culture tank 100. The outer end of the probe 300 is coaxially connected with a guide rod 400, and the guide rod 400 penetrates through the end wall of the sleeve 110 and is in sliding fit with the end wall of the sleeve 110.
The guide bar 400 has external threads and is threadedly engaged with a rotating sleeve 500, the rotating sleeve 500 being rotatably engaged with an end wall of the sleeve 110. The driving assembly 600 is mounted to an end wall of the sleeve 110, and a power output portion of the driving assembly 600 is in driving connection with the rotating sleeve 500.
The driving assembly 600 can drive the guide bar 400 to move along the length direction thereof by driving the rotating sleeve 500, thereby controlling the probe 300 to extend into the culture tank 100 or withdraw from the culture tank 100.
The inner wall of one side of the sleeve 110 near the bottom of the culture tank 100 is provided with a water spray pipe 120 and a blowing pipe 130, and the outlet parts of the water spray pipe 120 and the blowing pipe 130 are provided with pressurizing heads so that water and air can be conveniently sprayed out. When the probe 300 is positioned at the second sliding stop, the sprinkler pipe 120 and the blower pipe 130 are both disposed toward the notch 310.
After the PH is measured, the probe 300 slides from the first sliding dead point to the second sliding dead point, the shower pipe 120 sprays water into the notch 310 to clean the PH measuring electrode 200, and then the shower pipe 130 blows the notch 310 and the PH measuring electrode 200 dry. In this way, the residual liquid after the last measurement does not interfere with the next measurement, so that the trouble of manual cleaning is avoided, the sensitivity of the PH measuring electrode 200 is improved, the corrosion to the PH measuring electrode 200 is effectively reduced, and the service life of the PH measuring electrode 200 is prolonged.
Further, an inner wall of the sleeve 110 on one side near the bottom of the culture tank 100 has a recessed area 111, and the recessed area 111 is formed by recessing an inner wall of the sleeve 110. The depression 111 is filled with a sponge 112, the bottom of the depression 111 is provided with a drain 113, and the drain 113 has a joint for connection with a negative pressure device. After the PH measuring electrode 200 is washed, the washing water flows to the bottom side of the sleeve 110 and is discharged through the drain pipe 113. The sponge 112 can greatly improve the water adsorption, avoid residue, and the negative pressure device combined with the drain pipe 113 sufficiently sucks out the flushing water.
The bottom of the concave region 111 is provided with the diversion trenches 114, the diversion trenches 114 are formed by the bottom of the concave region 111 in a concave manner, and the diversion trenches 114 are radially distributed along the circumferential direction of the drain pipe 113. The diversion trench 114 can effectively improve the efficiency of water entering the drain pipe 113 from the concave area 111 and the sponge 112, and improve the drainage effect.
Further, referring to fig. 6, a sealing structure is provided between the probe 300 and the sidewall of the culture tank 100, and the sealing structure includes a first sealing ring 700 and a second sealing ring 800. The first sealing ring 700 and the second sealing ring 800 are fixedly connected to the side wall of the culture tank 100, the two first sealing rings 700 are respectively arranged on two sides of the second sealing ring 800, and the first sealing ring 700 and the second sealing ring 800 are respectively attached to the outer wall of the probe 300. The second seal ring 800 has a lumen 810 and an inflation conduit 820 for inflating the lumen 810.
The sealing effect of the second seal ring 800 and the stabilizing effect on the probe 300 can be greatly improved by inflating the inner cavity 810 of the second seal ring 800 with the inflation pipe 820.
When the probe 300 is in the moving process, the internal pressure of the second sealing ring 800 can be slightly reduced, so that the probe 300 can slide conveniently, and when the probe 300 slides in place, the internal pressure of the second sealing ring 800 is increased again, so that the probe 300 can be stabilized, and the sealing effect can be further improved.
Along the diameter of the second seal ring 800, the inner diameter a of the inner cavity 810 is one tenth to one eighth of the thickness b of the second seal ring 800. In this embodiment, the inner diameter a of the inner cavity 810 is one-ninth the thickness b of the second seal ring 800. By the design, the inner cavity 810 does not negatively affect the overall strength of the second sealing ring 800, the inner cavity 810 is pressurized, the overall structure of the second sealing ring 800 is not damaged, and the second sealing ring 800 can exert a stronger sealing effect while maintaining the original structure.
The inner side and the outer side of the sidewall of the culture pot 100 are provided with stopper blocks 140 for preventing the first sealing ring 700 from being separated, and the end of the first sealing ring 700 is attached to the end of the second sealing ring 800. When the inner cavity 810 of the second sealing ring 800 is inflated and pressurized, the bonding force between the second sealing ring 800 and the first sealing ring 700 can be further improved, the first sealing ring 700 is extruded, and the sealing and stabilizing effects of the first sealing ring 700 on the probe 300 are also enhanced under the co-extrusion action of the stop block 140 and the second sealing ring 800.
In this embodiment, the PH measuring electrode 200 is located in the notch 310 of the probe 300, and the notch 310 is located at the lower part of the needle body of the probe 300, so that it is ensured that the PH measuring electrode 200 is not easy to contact with the residual liquid before the PH measuring electrode 200 is cleaned.
Both probe 300 and guide bar 400 are hollow structures for the passage of wires of pH measuring electrode 200.
The present embodiment also provides a culture method using the liquid strain culture device 1000, which includes: the probe 300 is driven from the second sliding dead point to the first sliding dead point by the driving assembly 600 to perform the PH measurement. After the measurement is completed, the driving assembly 600 is used for driving the probe 300 from the first sliding dead point to the second sliding dead point, the water spraying pipe 120 is used for spraying water into the notch 310 for cleaning, and then the air blowing pipe 130 is used for blowing the notch 310 to dry.
In summary, the liquid strain culture device 1000 has a simple structure and convenient use, can accurately measure the pH value of the culture system, has higher sensitivity and accuracy, and is beneficial to accurately controlling the pH value of the culture system. The culture method is convenient to operate, can accurately measure the pH value of the culture system, has higher sensitivity and accuracy, and is favorable for accurately controlling the pH value of the culture system.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A liquid spawn culture device for pleurotus eryngii, comprising: a culture tank and a PH measurement assembly;
the PH measuring assembly comprises a plurality of PH measuring electrodes and probes, wherein the PH measuring electrodes are arranged in the probes, and notches for exposing the PH measuring electrodes are formed in the side walls of the probes; the probe is arranged along the diameter direction of the culture tank and is close to the tank bottom of the culture tank, the probe penetrates through the side wall of the culture tank and is in sliding fit with the side wall of the culture tank, the probe and the side wall of the culture tank are in sliding seal, and the probe is driven by the driving component;
the probe is provided with a first sliding dead point and a second sliding dead point, when the probe is positioned at the first sliding dead point, the notch is positioned inside the culture tank, and when the probe is positioned at the second sliding dead point, the notch is positioned outside the culture tank;
a sleeve is arranged on the outer side of the culture tank, and the sleeve is covered on the side wall of the culture tank;
the inner wall of one side of the sleeve, which is close to the bottom of the culture tank, is provided with a water spray pipe and a gas blowing pipe, and when the probe is positioned at the second sliding dead point, the water spray pipe and the gas blowing pipe are both arranged towards the notch;
the inner wall of one side of the sleeve, which is close to the bottom of the culture tank, is provided with a concave area, and the concave area is formed by concave inner wall of the sleeve; the concave area is filled with sponge, a drain pipe is arranged at the bottom of the concave area, and the drain pipe is provided with a joint for connecting with a negative pressure device;
the bottom of the concave area is provided with diversion trenches, the diversion trenches are formed by the bottom of the concave area in a concave mode, and a plurality of diversion trenches are radially distributed along the circumference of the drain pipe.
2. The liquid seed culture apparatus according to claim 1, wherein a plurality of the probes are arranged at regular intervals along a circumferential direction of the culture tank.
3. The liquid spawn-running device according to claim 1, characterized in that the outer end of the probe is coaxially connected with a guide rod, which penetrates through the end wall of the sleeve and is in sliding fit with the end wall of the sleeve; the guide rod is provided with external threads and is in threaded fit with a rotating sleeve, and the rotating sleeve is in rotatable fit with the end wall of the sleeve; the driving assembly is arranged on the end wall of the sleeve, and the power output part of the driving assembly is in transmission connection with the rotating sleeve.
4. The liquid spawn-up device of claim 1, characterized in that a sealing structure is provided between the probe and the side wall of the culture tank, the sealing structure comprising a first sealing ring and a second sealing ring; the first sealing ring and the second sealing ring are fixedly connected to the side wall of the culture tank, the two first sealing rings are respectively arranged on two sides of the second sealing ring, and the first sealing ring and the second sealing ring are respectively attached to the outer wall of the probe; the second seal ring has an inner cavity and an inflation conduit for inflating the inner cavity.
5. The liquid seed culture apparatus according to claim 4, wherein the inner diameter of the inner cavity is one tenth to one eighth of the thickness of the second sealing ring along the diameter direction of the second sealing ring.
6. The liquid spawn-running device according to claim 4, characterized in that the inner side and the outer side of the side wall of the culture tank are provided with stop blocks for preventing the first sealing ring from falling out, and the end part of the first sealing ring is attached to the end part of the second sealing ring.
7. A culture method using the liquid seed culture apparatus according to any one of claims 1 to 6, comprising:
driving the probe from the second sliding dead point to the first sliding dead point by using the driving assembly, and measuring the PH value;
after the measurement is finished, the driving assembly is used for driving the probe from the first sliding dead point to the second sliding dead point, the water spraying pipe is used for spraying water into the notch to clean, and then the blowing pipe is used for blowing the notch to dry.
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| CN210458145U (en) * | 2019-07-08 | 2020-05-05 | 重庆师范大学 | Automatic PH adjusting device |
| CN210860370U (en) * | 2019-10-21 | 2020-06-26 | 南京优格环保科技有限公司 | PH measuring device capable of ascending and descending along with liquid level for sewage treatment |
| CN110823981A (en) * | 2019-11-22 | 2020-02-21 | 铜陵有色金属集团股份有限公司 | Device and method for automatically cleaning PH electrode |
| CN212514574U (en) * | 2020-07-03 | 2021-02-09 | 永仁野森达菌业有限公司 | Truffle base soil pH value testing arrangement |
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