CN114958567A - Sterile inoculation device for fermentation tank and use method thereof - Google Patents
Sterile inoculation device for fermentation tank and use method thereof Download PDFInfo
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- CN114958567A CN114958567A CN202210759438.2A CN202210759438A CN114958567A CN 114958567 A CN114958567 A CN 114958567A CN 202210759438 A CN202210759438 A CN 202210759438A CN 114958567 A CN114958567 A CN 114958567A
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Abstract
The invention discloses a fermentation tank sterile inoculation device and a use method thereof; the invention comprises a tank body, a cleaning chamber, a conduit arranged in the tank body and extending to the outside, an in-pipe sterilizing component arranged on the surface of the conduit, an aseptic inoculation component arranged at one end of the conduit and an outside-pipe cleaning component arranged in the cleaning chamber, wherein the in-pipe cleaning component comprises a vertical pipe, steam is transmitted into the conduit through a steam port for sterilization, the aseptic inoculation component comprises an aseptic air pipe and an inoculation bottle, a second valve is opened, a third valve and a fourth valve in a cleaning box are respectively opened, the inoculation conduit is sterilized by steam, steam condensate water is discharged out of the cleaning chamber through the conduit, after the sterilization of the inoculation conduit is finished, the fourth valve is closed, then the third valve is closed, finally the second valve is closed, an exhaust valve is closed, the conduit is in an aseptic state, the integral operation difficulty is low, the risk of contamination is low, and the sterilizing function is realized for the inside of the conduit, thereby reducing the hazard.
Description
Technical Field
The invention relates to the technical field of fermentation tank inoculation equipment, in particular to a fermentation tank sterile inoculation device and a using method thereof.
Background
A fermenter refers to an apparatus used industrially for carrying out microbial fermentation. Generally, the main body is a main cylinder made of stainless steel plate, and the volume is from 1m to hundreds of m under thin-walled cultivation. It should be noted that the structure is strict, reasonable in design and processing, can tolerate steam sterilization, have certain operation elasticity, interior annex minimize (avoid the dead angle), material and energy transfer performance are strong to can carry out certain regulation so that wash, pollution abatement, be suitable for the production of multiple product and reduce energy consumption, the fermentation cylinder wide application is in trades such as dairy products, beverage, bioengineering, pharmacy, fine chemistry industry, the jar body is equipped with intermediate layer, heat preservation, heatable, cooling, heat preservation. The jar body all adopts spinning R angle processing with upper and lower fill head (or toper), and the jar inner wall is handled through mirror finish, no sanitary dead angle, and totally closed design ensures that the material is located all the time and is mixed, the fermentation under the pollution-free state, and equipment is equipped with the air breathing pore, and the CIP washs the shower nozzle, devices such as manhole, the classification of fermentation cylinder: according to the equipment of the fermentation tank, the fermentation tank is divided into a mechanical stirring ventilation fermentation tank and a non-mechanical stirring ventilation fermentation tank.
The invention relates to a biological fermentation equipment component, in particular to a liquid fermentation tank case inoculation device, which belongs to the patent number CN 103382440A. The device comprises an inoculation bottle, wherein a bottle cap of the inoculation bottle is hermetically connected with an inoculation pipe with a diaphragm valve and a sterile filtering respirator; the inoculation pipe is connected with an inlet of a sterile air inlet pipe on a fermentation tank through a section of middle pipeline, and the inlet of the sterile air inlet pipe is also provided with a branch connected with a sterile air source; the fermentation tank is also provided with a sterile air outlet pipe. The inoculation bottle and the fermentation tank are arranged, so that the contact probability of the environment in the fermentation tank and the outside air is reduced, the pollution of mixed bacteria is avoided, and the success rate of inoculation is improved.
However, there are some problems in the use of the existing fermentation tank inoculation equipment, 1, the fermentation tank inoculation equipment on the market at present directly pours the seed liquid into the fermentation tank under the protection of flame in an unclean area, the operation difficulty is high, the risk of contamination is high, and a function of sterilizing the interior of the fermentation tank is lacked, so that the hazard is increased, 2, the fermentation tank inoculation equipment on the market at present lacks a process measure capable of performing direct aseptic inoculation, so that the risk of contamination is increased in the inoculation process, and unnecessary waste is caused, 3, the fermentation tank inoculation equipment on the market at present also lacks a measure capable of performing real-time disinfection and sterilization on the surface of the equipment, so that the overall safety is reduced.
Disclosure of Invention
The invention aims to provide a sterile inoculation device for a fermentation tank and a using method thereof, which aim to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: the device comprises a tank body, a cleaning chamber, a conduit arranged in the tank body and extending to the outside, an in-tube sterilization assembly arranged on the surface of the conduit, a sterile inoculation assembly arranged at one end of the conduit and an outside-tube cleaning assembly arranged in the cleaning chamber;
the pipe cleaning component comprises a vertical pipe, the surface of the vertical pipe is connected with a second valve in a matching way, one end of the vertical pipe is detachably connected with a steam port, one end of the guide pipe is detachably connected with a third valve, one end of the guide pipe is bent and folded, the surface of the bent and folded part of the guide pipe is connected with a fourth valve in a matching way, and the steam port is used for steam transmission sterilization in the guide pipe;
aseptic inoculation subassembly includes aseptic air pipe and inoculation bottle, pipe one end can be dismantled and is connected with first union, first union one end cooperation is connected with the second union, second union one end is provided with the hose, through aseptic air pipe transmission aseptic air is impressed the pipe through the hose to the seed liquid in the inoculation bottle.
Preferably, the sterile inoculation assembly further comprises a fifth valve arranged on the surface of the hose, a sixth valve is arranged on the surface of the sterile air pipe, and a first valve is arranged at one end, close to the tank body, of the guide pipe.
Preferably, a housing is detachably connected to the inside of the cleaning chamber.
Preferably, the outside of tubes cleaning assembly includes a water storage tank detachably connected inside the housing, the water storage tank being provided inside with a water pump.
Preferably, the water pump one end can be dismantled the connection and be connected with the water pipe, the water pipe surface can be dismantled and be connected with atomizer.
Preferably, the atomizing spray heads are multiple, and an L pipe is connected to one side of the water storage tank in an embedded mode.
Preferably, the L pipe is connected to the outer side of the shell in a penetrating mode, and one end of the L pipe is detachably connected with the funnel.
Preferably, the top of the surface of the cleaning chamber is provided with a connecting hole.
Preferably, the connecting holes are located four corners of the top of the clean room, the connecting columns are connected to the inside of the connecting holes in a matched mode, the cover plate is fixedly connected to the tops of the connecting columns, the exhaust pipe is arranged on one side, located on one side of the tank body, of the surface of the guide pipe, and the micro-positive pressure device is arranged on the surface of the clean room.
Based on a fermentation tank sterile inoculation device and a using method thereof, the method comprises the following steps:
step one, when the cleaning box is used, the second valve is opened, then the third valve and the fourth valve in the cleaning box are respectively opened, steam is used for sterilizing the inoculation guide pipe, steam condensate water is discharged out of the cleaning chamber through the guide pipe, no condensate water is formed in the cleaning chamber, after the inoculation guide pipe is sterilized, the fourth valve is closed, then the third valve is closed, finally the second valve is closed, and at the moment, the guide pipe is in a sterile state;
step two, detaching the first movable joint, connecting the movable joint of the hose of the inoculation bottle with the fourth valve, opening the sixth valve and the fifth valve at the moment, opening the third valve, finally opening the first valve, and pressing the seeds into the fermentation tank by using sterile air;
step three, when sterilization is carried out, the water pump can be controlled through an external power supply, the water pump works to pump out the sterilization liquid in the water storage tank, the sterilization liquid enters the water pipe, and finally the sterilization liquid is discharged from a plurality of atomizing nozzles arranged on the surface in the water pipe to carry out rapid sterilization on the interior of the clean room;
step four, the water storage tank is connected and placed through the shell, and is filled with a disinfection solution and the like through the L pipe, and the L pipe is filled through a funnel arranged at one end;
and step five, the cleaning chamber is connected with the connecting column through a plurality of connecting holes formed in the top of the cleaning chamber, so that the cover plate can cover the surface of the cleaning chamber and is isolated from the outside.
Compared with the prior art, the invention has the beneficial effects that:
1. when the sterilizing device is used, the second valve is opened, the third valve and the fourth valve in the clean box are opened respectively, the inoculating guide pipe is sterilized by steam, steam condensate water is discharged out of the clean room through the guide pipe, no condensate water is formed in the clean room, after the inoculating guide pipe is sterilized, the fourth valve is closed first, then the third valve is closed, finally the second valve is closed, the exhaust valve is closed, the guide pipe is in an aseptic state, so that the inoculating device of the fermentation tank is poured into the fermentation tank in an aseptic environment, the operation difficulty is low, the risk of contamination is low, the sterilizing device has a function of sterilizing the interior of the inoculating guide pipe, and the hazard is reduced.
2. The first movable joint is detached, the movable joint of the inoculation bottle hose connected with the fourth valve is connected, so that the convenient connectivity is increased, the overall connectivity is improved, the sixth valve and the fifth valve are opened at the moment, the third valve is opened again, the first valve is opened finally, the seed liquid is pressed into the fermentation tank by using sterile air, and the fermentation tank inoculation equipment has a process measure capable of performing direct sterile inoculation, so that the contamination risk is reduced in the inoculation process, and the unnecessary waste is reduced.
3. When sterilization is carried out, the water pump can be controlled by an external power supply, the water pump works to pump out the disinfection and sterilization liquid in the water storage tank, the disinfection and sterilization liquid enters the water pipe and is finally discharged from the plurality of atomizing nozzles arranged on the surface of the water pipe, the interior of the clean room is sterilized quickly to achieve the effect of quick sterilization, the water storage tanks are connected and placed through the shell, the L-shaped pipe is filled through a funnel arranged at one end, the cleaning chamber is connected with the connecting column through a plurality of connecting holes arranged at the top part, so that the cover plate can cover the surface of the cleaning chamber, the inoculation device of the fermentation tank is isolated from the outside, and then the inoculation device of the fermentation tank has a measure for real-time disinfection and sterilization on the surface of the device, so that the overall safety is improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a fermenter sterile inoculation apparatus and a method for using the same according to the present invention;
FIG. 2 is a schematic view of a first movable joint structure of a sterile inoculation device for a fermentation tank and a use method thereof;
FIG. 3 is a schematic diagram of the internal structure of a clean room of an aseptic inoculation device for a fermentation tank and a use method thereof;
FIG. 4 is a schematic diagram of the internal structure of the housing of the sterile inoculation device for the fermentation tank and the use method thereof;
FIG. 5 is a schematic diagram of a cover plate structure of a sterile inoculation device for a fermentation tank and a use method thereof.
In the figure: 1. a tank body; 2. a conduit; 3. a first valve; 401. a vertical tube; 402. a second valve; 403. a steam port; 404. a third valve; 405. a fourth valve; 501. a first movable joint; 502. a second movable joint; 503. a hose; 504. a fifth valve; 505. inoculating bottles; 506. a sterile air tube; 507. a sixth valve; 6. connecting holes; 7. a housing; 801. a water storage tank; 802. a water pump; 803. a water pipe; 804. an atomizing spray head; 805. an L pipe; 806. a funnel; 9. connecting columns; 10. a cover plate; 11. an exhaust pipe; 12. a clean room; 13. a micro positive pressure device.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Referring to fig. 1-5, a schematic overall structure of a fermenter sterile inoculation apparatus and a method for using the same includes a tank 1, a clean room 12, a conduit 2 disposed inside the tank 1 and extending to the outside, an in-tube sterilization assembly disposed on the surface of the conduit 2, a sterile inoculation assembly disposed at one end of the conduit 2, and an outside-tube cleaning assembly disposed inside the clean room 12;
the in-pipe cleaning assembly comprises a vertical pipe 401, a second valve 402 is connected to the surface of the vertical pipe 401 in a matching mode, a steam port 403 is detachably connected to one end of the vertical pipe 401, a third valve 404 is detachably connected to one end of the guide pipe 2, one end of the guide pipe 2 is bent and folded, a fourth valve 405 is connected to the surface of the bent and folded part of the guide pipe 2 in a matching mode, and steam is transmitted into the guide pipe 2 through the steam port 403 for sterilization;
the sterile inoculation assembly comprises a sterile air pipe 506 and an inoculation bottle 505, one end of the conduit 2 is detachably connected with a first movable joint 501, one end of the first movable joint 501 is connected with a second movable joint 502 in a matching mode, one end of the second movable joint 502 is provided with a hose 503, and seed liquid in the inoculation bottle 505 is pressed into the conduit 2 through the hose 503 by conveying sterile air through the sterile air pipe 506.
Specifically, the sterile inoculation assembly further comprises a fifth valve 504 arranged on the surface of the hose 503, a sixth valve 507 arranged on the surface of the sterile air pipe 506, and a first valve 3 arranged at one end of the conduit 2 close to the tank body 1.
Specifically, the housing 7 is detachably attached to the inside of the clean room 12.
Specifically, the pipe outside cleaning assembly comprises a water storage tank 801 detachably connected to the inside of the housing 7, and a water pump 802 is arranged inside the water storage tank 801.
Specifically, one end of the water pump 802 is detachably connected with a water pipe 803, and the surface of the water pipe 803 is detachably connected with an atomizing nozzle 804.
Specifically, a plurality of atomizing nozzles 804 are provided, and an L pipe 805 is connected to one side of the water storage tank 801 in an embedded manner.
Specifically, the L-shaped tube 805 is connected to the outside of the housing 7, and one end of the L-shaped tube 805 is detachably connected to the funnel 806.
Specifically, the top of the surface of the cleaning chamber 12 is provided with a connecting hole 6.
Specifically, connecting hole 6 is located 12 top four corners in clean room, and connecting post 9 is connected to connecting hole 6 inside cooperation, and connecting post 9 top fixedly connected with apron 10, 2 surfaces of pipe are located 1 one side of jar body and are provided with blast pipe 11, and clean room 12 surface is provided with pressure-fired 13 device.
A use method of the sterile inoculation device of the fermentation tank comprises the following steps:
step one, when in use, the second valve 402 is opened, then the third valve 404 and the fourth valve 405 in the clean box are respectively opened, steam is used for sterilizing the inoculation conduit 2, steam condensate water is discharged out of the clean room 12 through the conduit 2, no condensate water is formed in the clean room 12, after the inoculation conduit 2 is sterilized, the fourth valve 405 is closed, then the third valve 404 is closed, finally the second valve 402 is closed, and at the moment, the conduit 2 is in a sterile state;
step two, the first movable joint 501 is detached, the flexible pipe 503 of the inoculation bottle 505 is connected with the movable joint of the fourth valve 405, at the moment, the sixth valve 507 and the fifth valve 504 are opened, the third valve 404 is opened, finally, the first valve 3 is opened, and the seeds are hydraulically pressed into the fermentation tank by using sterile air;
step three, when sterilization is carried out, the water pump 802 can be controlled through an external power supply, the water pump 802 works to pump out the sterilization liquid in the water storage tank 801, the sterilization liquid enters the water pipe 803, and finally the sterilization liquid is discharged from a plurality of atomizing nozzles 804 arranged on the surface of the water pipe 803, so that the inside of the cleaning chamber 12 is sterilized quickly;
step four, the water storage tank 801 is connected and placed through the shell 7, the L-shaped pipe 805 is filled with disinfection and sterilization liquid and the like, and the L-shaped pipe 805 is filled through a funnel 806 arranged at one end of the L-shaped pipe 805;
and step five, the cleaning chamber 12 is connected with the connecting column 9 through a plurality of connecting holes 6 formed in the top, so that the cover plate 10 can cover the surface of the cleaning chamber 12 and isolate the surface from the outside.
When the sterilizing tank is used, the second valve 402 is opened, the third valve 404 and the fourth valve 405 in the clean box are opened respectively, the inoculating conduit 2 is sterilized by steam, the steam condensate water is discharged out of the clean chamber 12 through the conduit 2, no condensate water is formed in the clean chamber 12, after the sterilizing of the inoculating conduit 2 is finished, the fourth valve 405 is closed, the third valve 404 is closed, finally the second valve 402 is closed, the exhaust valve is closed, the conduit 2 is in a sterile state, so that the inoculating equipment of the fermenting tank is poured into the fermenting tank in a sterile environment, the operation difficulty is low, the risk of contamination is low, the inoculating equipment has a function of sterilizing the inside of the inoculating tank, the hazard is reduced, the first movable joint 501 is detached, the hose 503 of the inoculating bottle 505 is connected with the movable joint of the fourth valve 405, the convenient connectivity is increased, and the overall connectivity of the inoculating tank is improved, at the moment, the sixth valve 507 and the fifth valve 504 are opened, the third valve 404 is opened, the first valve 3 is opened finally, the seed liquid is pressed into the fermentation tank by sterile air, the fermentation tank inoculation device has a process measure for direct sterile inoculation, so that the risk of bacterial contamination is reduced in the inoculation process, unnecessary waste is reduced, when sterilization is performed, the water pump 802 can be controlled by an external power supply, the water pump 802 works to pump out the disinfection and sterilization liquid in the water storage tank 801 to enter the water pipe 803, and finally the disinfection and sterilization liquid is discharged from a plurality of atomizing nozzles 804 arranged on the surface of the water pipe 803 to perform rapid disinfection and sterilization on the inside of the clean room 12, so that the effect of rapid sterilization is achieved, the water storage tank 801 is connected and placed through the shell 7, and is subjected to disinfection and sterilization liquid filling and sterilization through the L pipe 805, so on convenience in use is increased, the L pipe 805 is filled through a funnel 806 arranged at one end, the cleaning chamber 12 is connected with a connecting column 9 through a plurality of connecting holes 6 formed in the top, so that the cover plate 10 can cover the surface of the cleaning chamber 12 and is isolated from the outside, the sterile air passing through the micro-positive pressure device 13 is ensured to be always in a sterile state in the cleaning chamber 12, and then the fermentation tank inoculation equipment also has a measure for real-time disinfection and sterilization on the surface of the equipment, so that the overall safety is improved.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a sterile inoculation device of fermentation cylinder which characterized in that: the device comprises a tank body (1), a cleaning chamber (12), a conduit (2) which is arranged in the tank body (1) and extends to the outside, an in-pipe sterilization assembly arranged on the surface of the conduit (2), a sterile inoculation assembly arranged at one end of the conduit (2) and an outside-pipe cleaning assembly arranged in the cleaning chamber (12);
the in-pipe cleaning assembly comprises a vertical pipe (401), a second valve (402) is connected to the surface of the vertical pipe (401) in a matching mode, a steam port (403) is detachably connected to one end of the vertical pipe (401), a third valve (404) is detachably connected to one end of the guide pipe (2), one end of the guide pipe (2) is bent and folded, a fourth valve (405) is connected to the surface of the bent and folded part of the guide pipe (2) in a matching mode, and steam is transmitted into the guide pipe (2) through the steam port (403) for sterilization;
aseptic inoculation subassembly includes aseptic air pipe (506) and inoculation bottle (505), pipe (2) one end can be dismantled and is connected with first union (501), first union (501) one end cooperation is connected with second union (502), second union (502) one end is provided with hose (503), through aseptic air pipe (506) transmission aseptic air is impressed the seed liquid in inoculation bottle (505) in pipe (2) through hose (503).
2. The aseptic inoculating device of fermenter according to claim 1, wherein: the aseptic inoculation subassembly still includes and sets up in fifth valve (504) on hose (503) surface, aseptic air pipe (506) surface is provided with sixth valve (507), pipe (2) are close to jar body (1) one end and are provided with first valve (3).
3. The aseptic inoculating device of fermenter according to claim 2, wherein: the cleaning chamber (12) is detachably connected with a shell (7).
4. The aseptic inoculating device of fermenter according to claim 2, wherein: the external pipe cleaning assembly comprises a water storage tank (801) detachably connected to the inside of the shell (7), and a water pump (802) is arranged inside the water storage tank (801).
5. The aseptic inoculating device of fermenter according to claim 4, wherein: water pump (802) one end can be dismantled to connect and be connected with water pipe (803), water pipe (803) surface can be dismantled and is connected with atomizer (804).
6. The aseptic inoculating device of fermenter according to claim 4, wherein: the atomizing spray heads (804) are multiple, and an L pipe (805) is embedded and connected to one side of the water storage tank (801).
7. The aseptic inoculating device of a fermenter according to claim 6, wherein: the L pipe (805) is connected to the outer side of the shell (7) in a penetrating mode, and one end of the L pipe (805) is detachably connected with the funnel (806).
8. The aseptic inoculating device of fermenter according to claim 7, wherein: the top of the surface of the cleaning chamber (12) is provided with a connecting hole (6).
9. The aseptic inoculation device of fermentation tank and its use method as claimed in claim 1, wherein: connecting hole (6) are located clean room (12) top four corners, connecting hole (6) inside cooperation is connected with spliced pole (9), spliced pole (9) top fixedly connected with apron (10), pipe (2) surface is located jar body (1) one side and is provided with blast pipe (11), clean room (12) surface is provided with pressure-fired device (13).
10. The use method of the aseptic inoculation device for fermenter according to any one of claims 1 to 9, wherein: the method comprises the following steps:
step one, when the cleaning box is used, a second valve (402) is opened, then a third valve (404) and a fourth valve (405) in the cleaning box are respectively opened, steam is used for sterilizing the inoculation guide pipe (2), steam condensate water is discharged out of the cleaning chamber (12) through the guide pipe (2), no condensate water is formed in the cleaning chamber (12), after the inoculation guide pipe (2) is sterilized, the fourth valve (405) is closed, then the third valve (404) is closed, finally the second valve (402) is closed, and at the moment, the guide pipe (2) is in a sterile state;
step two, the first movable joint (501) is detached, the hose (503) of the inoculation bottle (505) is connected with the movable joint of the fourth valve (405), at the moment, the sixth valve (507) and the fifth valve (504) are opened, the third valve (404) is opened, finally, the first valve (3) is opened, and seeds are hydraulically pressed into the fermentation tank by using sterile air;
step three, when sterilization is carried out, the water pump (802) can be controlled through an external power supply, the water pump (802) works to pump out the sterilization liquid in the water storage tank (801) and enter the water pipe (803), and finally the sterilization liquid is discharged from a plurality of atomizing nozzles (804) arranged on the surface of the water pipe (803) to carry out rapid sterilization on the inside of the cleaning room (12);
step four, the water storage tank (801) is connected and placed through the shell (7), and is filled with a disinfection solution and the like through the L pipe (805), and the L pipe (805) is filled through a funnel (806) arranged at one end;
and step five, the cleaning chamber (12) is connected with the connecting column (9) through a plurality of connecting holes (6) formed in the top of the cleaning chamber, so that the cover plate (10) can cover the surface of the cleaning chamber (12) and is isolated from the outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210759438.2A CN114958567A (en) | 2022-06-30 | 2022-06-30 | Sterile inoculation device for fermentation tank and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210759438.2A CN114958567A (en) | 2022-06-30 | 2022-06-30 | Sterile inoculation device for fermentation tank and use method thereof |
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| CN114958567A true CN114958567A (en) | 2022-08-30 |
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| CN202210759438.2A Pending CN114958567A (en) | 2022-06-30 | 2022-06-30 | Sterile inoculation device for fermentation tank and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115678749A (en) * | 2022-10-17 | 2023-02-03 | 安琪酶制剂(宜昌)有限公司 | Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method |
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2022
- 2022-06-30 CN CN202210759438.2A patent/CN114958567A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115678749A (en) * | 2022-10-17 | 2023-02-03 | 安琪酶制剂(宜昌)有限公司 | Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method |
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