CN115678749A - Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method - Google Patents
Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method Download PDFInfo
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- CN115678749A CN115678749A CN202211268230.7A CN202211268230A CN115678749A CN 115678749 A CN115678749 A CN 115678749A CN 202211268230 A CN202211268230 A CN 202211268230A CN 115678749 A CN115678749 A CN 115678749A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 183
- 239000007788 liquid Substances 0.000 title claims abstract description 112
- 238000000855 fermentation Methods 0.000 title claims abstract description 88
- 230000004151 fermentation Effects 0.000 title claims abstract description 88
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 65
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 51
- 230000001580 bacterial effect Effects 0.000 claims abstract description 13
- 238000007599 discharging Methods 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000011109 contamination Methods 0.000 abstract description 8
- 239000002054 inoculum Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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Abstract
The invention provides a fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and a using method thereof, the fermentation tank inoculation system comprises a fermentation tank, wherein the top of the fermentation tank is communicated with a steam supply system for introducing high-temperature steam for sterilization; the top of the fermentation tank is communicated with a steam discharge system for discharging steam; the top of the fermentation tank is communicated with an inoculation liquid inlet system for injecting inoculation liquid; the inoculation liquid inlet system is connected with the inoculation tank, and the inoculation tank is communicated with a pressure inoculation system which is used for injecting sterilization air and pressurizing the inoculation tank. The inoculation system can provide a relatively closed inoculation environment, and effectively prevents the risk of bacterial contamination in the inoculation process; the system can perform inoculation after sterilization by virtue of a sterilization system of the fermentation tank, and plays a role in sterilization; meanwhile, a positive pressure inoculation mode is adopted, so that the inoculation process is simplified.
Description
Technical Field
The invention relates to the technical field of strain inoculation, in particular to a fermentation tank inoculation system convenient for sterilization and positive-pressure liquid supply and a using method thereof.
Background
The microbial fermentation production needs to culture seeds, the seeds cultured in a strain chamber need to be transferred to a seed fermentation tank in production, then seed amplification culture is carried out, the transfer process needs aseptic operation, and fermentation failure can be caused once fermentation contamination is caused by abnormal operation.
The current process of inoculating a fermenter is: set up open inoculation feed liquor hopper through the top at the fermentation cylinder, the inoculation jar after will disinfecting is inside to be disinfected to hold the fungus liquid inside the inoculation, inside the mode of rethread dumping will inoculate the inside fungus liquid of jar and inject into the fermentation cylinder through the inoculation feed liquor hopper at fermentation tank top, finally accomplish the inoculation process. In the process, in order to ensure a sterile inoculation environment, a fire ring is required to be arranged on the periphery of the open inoculation liquid inlet hopper in the process of introducing the strain liquid. The inoculation process can not thoroughly ensure the sterile environment, has certain risk of contamination, and can bring certain potential safety hazards due to fire in the operation process.
Disclosure of Invention
The invention overcomes the defects of the prior art, and provides the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and the use method thereof, the inoculation system can provide a relatively closed inoculation environment, and the risk of bacterial contamination in the inoculation process is effectively prevented; the system can perform inoculation after sterilization by virtue of a sterilization system of the fermentation tank, and plays a role in sterilization; meanwhile, a positive pressure inoculation mode is adopted, so that the inoculation process is simplified.
In order to achieve the technical characteristics, the invention aims to realize that: the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply comprises a fermentation tank, wherein the top of the fermentation tank is communicated with a steam supply system for introducing high-temperature steam for sterilization; the top of the fermentation tank is communicated with a steam discharge system for discharging steam; the top of the fermentation tank is communicated with an inoculation liquid inlet system for injecting inoculation liquid; the inoculation liquid inlet system is connected with the inoculation tank, and the inoculation tank is communicated with a pressure inoculation system which is used for injecting sterilization air and pressurizing the inoculation tank.
The fermentation tank is integrally supported and installed at the top of the fermentation tank base.
The steam supply system comprises a main steam pipe, and the main steam pipe is communicated with an inner steam pipe arranged in the fermentation tank through a first steam valve.
The steam discharging system comprises a steam discharging pipe fixed at the top of the fermentation tank, a second steam valve is installed on the steam discharging pipe and connected with a steam communicating pipe through a first movable joint, a third steam valve is installed on the steam communicating pipe, and the steam communicating pipe is connected with the inoculation liquid inlet system.
The inoculation liquid entering system comprises a liquid inlet pipe communicated with the top of the fermentation tank, a first liquid inlet valve is installed on the liquid inlet pipe and is connected with a second liquid inlet pipe through a second movable joint, a second liquid inlet valve is installed on the second liquid inlet pipe, the bottom end of the second liquid inlet pipe is connected with a pipe bottom transverse pipe, and the pipe bottom transverse pipe is tightly attached to the inner bottom end of the inoculation tank.
The pressure inoculation system comprises an aseptic air communicating pipe communicated with the top of the inoculation tank, and the aseptic air communicating pipe is connected with an aseptic air pipe through an aseptic air control valve.
The steam communicating pipe is communicated with a second liquid inlet pipe of the inoculation liquid inlet system, and the communicating part is positioned between the second liquid inlet valve and the second movable joint.
The use method of the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply comprises the following steps:
step one, connecting a system pipeline: connecting the fermentation tank with an inoculation tank;
step two, introducing steam to sterilize the system: sterilizing the fermentation tank and the inoculation tank after the communication through steam;
step three, pouring the inoculation liquid: after the sterilization is finished, pouring the bacterial liquid into an inoculation tank, and covering a top cover at the top of the inoculation tank;
step four, performing pressure inoculation on the bacterial liquid: inoculating the bacterial liquid in the inoculation tank to the fermentation tank in a pressurizing mode;
step five, disassembling the system: and after inoculation is completed, the first movable joint and the second movable joint are disassembled, and the inoculation tank is removed.
The use method of the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply comprises the following steps:
the specific process of system pipeline connection in the step one is as follows: a second liquid inlet pipe on the inoculation tank is communicated with a liquid inlet pipe through a second movable joint, and a steam communication pipe is communicated with a steam discharge pipe through a first movable joint;
the sterilization in the second step comprises the following specific processes: in the sterilization process, a first steam valve, a second steam valve, a third steam valve, a second liquid inlet valve and an aseptic air control valve are opened, the first liquid inlet valve is closed, steam sequentially enters a fermentation tank and a steam communicating pipe, enters a second liquid inlet pipe, and finally enters an inoculation tank to sterilize the whole system.
The specific process of the step four is as follows: and (3) opening the sterile air control valve, the second liquid inlet valve, the first steam valve and the first liquid inlet valve, closing the second steam valve and the third steam valve, injecting sterile air into the inoculation tank through the sterile air pipe, further pressurizing the inoculation tank, pressing the bacterial liquid into a second liquid inlet pipe and a liquid inlet pipe of an inoculation liquid inlet system from a pipe bottom transverse pipe after pressurization, and finally entering the fermentation tank to further complete the inoculation process.
The invention has the following beneficial effects:
1. the inoculation system can provide a relatively closed inoculation environment, and effectively prevents the risk of bacterial contamination in the inoculation process; the system can perform sterilization by virtue of a sterilization system of the fermentation tank and then perform inoculation, so as to achieve the sterilization effect; meanwhile, a positive pressure inoculation mode is adopted, so that the inoculation process is simplified.
2. Through foretell steam feed system can be used for providing the steam of fermentation cylinder sterilization through foretell, and then disinfect to the fermentation cylinder to can carry out corresponding sterilization with the help of this system to inoculation jar and inoculum entering system, guarantee aseptic inoculation process, and then guarantee the inoculation security, prevent the risk of taking place the contamination.
3. Through foretell steam escape system can link to each other the steam sterilization system of fermentation cylinder with the inoculation jar, and then carry out supplementary sterilization to the inoculation jar.
4. The inoculation liquid entering system can send the inoculation liquid in the inoculation tank into the fermentation tank, and therefore a closed sterile inoculation process is achieved. Can guarantee through the second loose joint that the inoculation jar can be dismantled, and then make it can remove for the inoculation operation of different positions fermentation cylinder.
5. Can be used for providing certain pressure for the inoculation jar is inside through pressure inoculation system, and then through the inside inoculum liquid of pressure with the inoculation jar cross tube of pipe bottom impress to the inoculum liquid entering system to finally send it into the inside of fermentation cylinder.
6. The steam communicating pipe can be conveniently communicated with the inoculation liquid inlet system, so that the sterilization process of the inoculation tank is facilitated.
Drawings
The invention is further illustrated by the following figures and examples.
FIG. 1 is a schematic diagram of the overall system of the present invention.
FIG. 2 is a flow chart of the overall system sterilization process of the present invention.
FIG. 3 is a schematic diagram of the overall system inoculation process of the present invention.
In the figure: fermentation cylinder base 1, fermentation cylinder 2, steam inner tube 3, steam are responsible for 4, first steam valve 5, steam calandria 6, second steam valve 7, first union joint 8, third steam valve 9, steam communicating pipe 10, feed liquor pipe 11, first feed liquor valve 12, second union joint 13, second feed liquor valve 14, second feed liquor pipe 15, the horizontal pipe 16 of socle, inoculation jar 17, top cap 18, aseptic air communicating pipe 19, aseptic air control valve 20, aseptic air pipe 21.
Detailed Description
Embodiments of the present invention will be further described with reference to the accompanying drawings.
Example 1:
referring to fig. 1-3, the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply comprises a fermentation tank 2, wherein the top of the fermentation tank 2 is communicated with a steam supply system for introducing high-temperature steam for sterilization; the top of the fermentation tank 2 is communicated with a steam discharge system for discharging steam; the top of the fermentation tank 2 is communicated with an inoculation liquid inlet system for injecting inoculation liquid; the inoculation liquid inlet system is connected with an inoculation tank 17, and the inoculation tank 17 is communicated with a pressure inoculation system which is used for injecting sterilization air and pressurizing the inoculation tank 17. The inoculation system can provide a relatively closed inoculation environment, and effectively prevents the risk of bacterial contamination in the inoculation process; the system can perform inoculation after sterilization by virtue of a sterilization system of the fermentation tank, and plays a role in sterilization; meanwhile, a positive pressure inoculation mode is adopted, so that the inoculation process is simplified. The inoculation system can provide a relatively closed inoculation environment, and effectively prevents the risk of bacterial contamination in the inoculation process; the system can perform inoculation after sterilization by virtue of a sterilization system of the fermentation tank, and plays a role in sterilization; meanwhile, a positive pressure inoculation mode is adopted, so that the inoculation process is simplified.
Further, the fermentation tank 2 is integrally supported and installed on the top of the fermentation tank base 1. The fermentation tank base 1 can be used for stably supporting and fixing the fermentation tank 2, and further ensures the stability of fixing the fermentation tank 2.
Further, the steam supply system comprises a main steam pipe 4, and the main steam pipe 4 is communicated with an inner steam pipe 3 arranged inside the fermentation tank 2 through a first steam valve 5. Can be used for providing the steam that fermentation cylinder 2 disinfected through foretell steam feed system, and then disinfect fermentation cylinder 2 to can carry out corresponding sterilization with the help of this system to inoculation jar 17 and inoculum entering system, guaranteed aseptic inoculation process, and then guaranteed the inoculation security, prevent the risk of taking place the microbiological contamination.
Further, the steam discharging system is including fixing the steam calandria 6 at 2 tops of fermentation cylinder, install second steam valve 7 on the steam calandria 6 to link to each other with steam communicating pipe 10 through first union joint 8, install third steam valve 9 on the steam communicating pipe 10, steam communicating pipe 10 links to each other with the inoculation liquid entering system. The steam sterilization system of the fermentation tank 2 can be connected with the inoculation tank 17 through the steam discharge system, so that the inoculation tank 17 can be sterilized in an auxiliary way.
Further, the inoculation liquid entering system comprises a liquid inlet pipe 11 communicated with the top of the fermentation tank 2, a first liquid inlet valve 12 is installed on the liquid inlet pipe 11 and is connected with a second liquid inlet pipe 15 through a second movable joint 13, a second liquid inlet valve 14 is installed on the second liquid inlet pipe 15, the bottom end of the second liquid inlet pipe 15 is connected with a pipe bottom transverse pipe 16, and the pipe bottom transverse pipe 16 is tightly attached to the inner bottom end of the inoculation tank 17. The inoculum inside the inoculum tank 17 can be fed into the interior of the fermentation tank 2 by means of the inoculum inlet system described above, thereby achieving a closed aseptic inoculation process. The second movable joint 13 can ensure that the inoculation tank 17 can be detached, so that the inoculation tank can be moved for the inoculation operation of the fermentation tank at different positions.
Further, the pressure inoculation system comprises a sterile air communicating pipe 19 communicated with the top of the inoculation tank 17, and the sterile air communicating pipe 19 is connected with a sterile air pipe 21 through a sterile air control valve 20. The pressure inoculation system can be used to provide a certain pressure inside the inoculation tank 17, by means of which the inoculum inside the inoculation tank 17 is pressed by pressure through the cross-tube at the bottom 16 into the inoculum inlet system and finally into the interior of the fermentation tank 2.
Further, the steam communicating pipe 10 is communicated with a second liquid inlet pipe 15 of the inoculation liquid inlet system, and the communicating part is positioned between a second liquid inlet valve 14 and a second movable joint 13. The steam communicating pipe 10 can be conveniently communicated with an inoculation liquid inlet system, so that the sterilization process of the inoculation tank 17 is facilitated.
Example 2:
the use method of the fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply comprises the following steps:
step one, connecting a system pipeline: connecting the fermentation tank 2 with an inoculation tank 17;
step two, introducing steam to sterilize the system: sterilizing the fermentation tank 2 and the inoculation tank 17 after the communication by steam;
step three, pouring the inoculation liquid: after the sterilization is finished, pouring the bacteria liquid into the inoculation tank 17, and covering a top cover 18 at the top of the inoculation tank;
step four, performing pressure inoculation on the bacterial liquid: inoculating the bacterial liquid in the inoculation tank 17 to the fermentation tank 2 in a pressurization mode;
step five, disassembling the system: after inoculation is completed, the first movable joint 8 and the second movable joint 13 are detached, and the inoculation tank 17 is removed.
Further, the specific process of connecting the system pipeline in the step one is as follows: a second liquid inlet pipe 15 on the inoculation tank 17 is communicated with a liquid inlet pipe 11 through a second movable joint 13, and a steam communicating pipe 10 is communicated with a steam discharge pipe 6 through a first movable joint 8;
further, the specific sterilization process in the second step is as follows: in the sterilization process, the first steam valve 5, the second steam valve 7, the third steam valve 9, the second liquid inlet valve 14 and the sterile air control valve 20 are opened, the first liquid inlet valve 12 is closed, steam sequentially enters the fermentation tank 2 and the steam communicating pipe 10, enters the second liquid inlet pipe 15, and finally enters the inoculation tank 17 to perform sterilization of the whole system.
Further, the specific process of the fourth step is as follows: and (3) opening the sterile air control valve 20, the second liquid inlet valve 14, the first steam valve 5 and the first liquid inlet valve 12, closing the second steam valve 7 and the third steam valve 9, injecting sterile air into the inoculation tank 17 through a sterile air pipe 21, further pressurizing the inoculation tank 17, pressing the bacteria liquid into the second liquid inlet pipe 15 and the liquid inlet pipe 11 of the inoculation liquid inlet system from the pipe bottom transverse pipe 16 after pressurization, and finally entering the fermentation tank 2, thereby completing the inoculation process.
Claims (10)
1. Fermentation cylinder inoculation system convenient to sterilization and malleation supply liquid, its characterized in that: the fermentation tank comprises a fermentation tank (2), wherein the top of the fermentation tank (2) is communicated with a steam supply system for introducing high-temperature steam for sterilization; the top of the fermentation tank (2) is communicated with a steam discharge system for discharging steam; the top of the fermentation tank (2) is communicated with an inoculation liquid inlet system for injecting inoculation liquid; the inoculation liquid inlet system is connected with an inoculation tank (17), and the inoculation tank (17) is communicated with a pressure inoculation system which is used for injecting sterilization air and pressurizing the inoculation tank (17).
2. The fermenter inoculation system for facilitating sterilization and positive pressure liquid supply of claim 1, wherein: the fermentation tank (2) is integrally supported and installed at the top of the fermentation tank base (1).
3. The fermenter inoculation system for facilitating sterilization and positive pressure liquid supply of claim 1, wherein: the steam supply system comprises a main steam pipe (4), wherein the main steam pipe (4) is communicated with an inner steam pipe (3) arranged in the fermentation tank (2) through a first steam valve (5).
4. The fermentor inoculation system for facilitating sterilization and positive pressure liquid supply of claim 1, wherein: the steam discharging system comprises a steam drain pipe (6) fixed at the top of the fermentation tank (2), a second steam valve (7) is installed on the steam drain pipe (6) and is connected with a steam communicating pipe (10) through a first movable joint (8), a third steam valve (9) is installed on the steam communicating pipe (10), and the steam communicating pipe (10) is connected with an inoculation liquid inlet system.
5. The fermenter inoculation system for facilitating sterilization and positive pressure liquid supply of claim 1 or 4, wherein: inoculation liquid entering system is including feed liquor pipe (11) of intercommunication at fermentation cylinder (2) top, install first feed liquor valve (12) on feed liquor pipe (11) to link to each other with second feed liquor pipe (15) through second union joint (13), install second feed liquor valve (14) on second feed liquor pipe (15), the bottom of second feed liquor pipe (15) is connected with the bobbin base and violently manages (16), the bobbin base is violently managed (16) and is hugged closely the inner bottom of inoculation jar (17).
6. The fermentor inoculation system for facilitating sterilization and positive pressure liquid supply of claim 1, wherein: the pressure inoculation system comprises a sterile air communicating pipe (19) communicated with the top of an inoculation tank (17), and the sterile air communicating pipe (19) is connected with a sterile air pipe (21) through a sterile air control valve (20).
7. The fermenter inoculation system for facilitating sterilization and positive pressure liquid supply of claim 4, wherein: the steam communicating pipe (10) is communicated with a second liquid inlet pipe (15) of the inoculation liquid inlet system, and the communicating part is positioned between a second liquid inlet valve (14) and a second movable joint (13).
8. The method of using the fermenter inoculation system for facilitating sterilization and positive pressure liquid supply of any one of claims 1 to 7, comprising the steps of:
step one, connecting a system pipeline: connecting the fermentation tank (2) with an inoculation tank (17);
step two, introducing steam to sterilize the system: sterilizing the fermentation tank (2) and the inoculation tank (17) after the communication through steam;
step three, pouring the inoculation liquid: after the sterilization is finished, pouring the bacteria liquid into an inoculation tank (17), and covering a top cover (18) at the top of the inoculation tank;
step four, carrying out pressure inoculation on the bacterial liquid: inoculating the bacterial liquid in the inoculation tank (17) to the fermentation tank (2) in a pressurization mode;
step five, disassembling the system: after inoculation is completed, the first movable joint (8) and the second movable joint (13) are disassembled, and the inoculation tank (17) is removed.
9. The method of using a fermentor inoculation system for facilitating sterilization and positive pressure liquid supply of claim 8, characterized by:
the specific process of system pipeline connection in the step one is as follows: a second liquid inlet pipe (15) on the inoculation tank (17) is communicated with a liquid inlet pipe (11) through a second movable joint (13), and a steam communicating pipe (10) is communicated with a steam discharge pipe (6) through a first movable joint (8);
the specific sterilization process in the second step comprises the following steps: in the sterilization process, a first steam valve (5), a second steam valve (7), a third steam valve (9), a second liquid inlet valve (14) and a sterile air control valve (20) are opened, the first liquid inlet valve (12) is closed, steam sequentially enters a fermentation tank (2) and a steam communicating pipe (10) and enters a second liquid inlet pipe (15), and finally enters an inoculation tank (17) to sterilize the whole system.
10. The method of using a fermentor inoculation system for facilitating sterilization and positive pressure liquid supply of claim 8, characterized by: the specific process of the fourth step is as follows: open aseptic air control valve (20), second feed liquor valve (14), first steam valve (5) and first feed liquor valve (12), close second steam valve (7) and third steam valve (9), through aseptic air pipe (21) to the inside injection aseptic air of inoculation jar (17), and then for inoculation jar (17) pressurization, second feed liquor pipe (15) and feed liquor pipe (11) that inoculation liquid got into the system will be impressed from horizontal pipe bottom pipe (16) to the fungus liquid after the pressurization, finally get into inside fermentation cylinder (2), and then accomplish the inoculation process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211268230.7A CN115678749A (en) | 2022-10-17 | 2022-10-17 | Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211268230.7A CN115678749A (en) | 2022-10-17 | 2022-10-17 | Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method |
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| CN115678749A true CN115678749A (en) | 2023-02-03 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100767569B1 (en) * | 2007-04-24 | 2007-10-17 | 김대환 | Fermentor |
| CN202369585U (en) * | 2011-12-27 | 2012-08-08 | 四川省申联生物科技有限责任公司 | Anaerobic inoculation tank |
| CN204385199U (en) * | 2014-12-31 | 2015-06-10 | 镇江东方生物工程设备技术有限责任公司 | A kind of inoculating device of fermentation tank |
| CN104911096A (en) * | 2015-06-10 | 2015-09-16 | 湖北五林中地农业科技有限公司 | Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique |
| CN114958567A (en) * | 2022-06-30 | 2022-08-30 | 江苏丰泽生物工程设备制造有限公司 | Sterile inoculation device for fermentation tank and use method thereof |
| CN218778943U (en) * | 2022-10-17 | 2023-03-31 | 安琪酶制剂(宜昌)有限公司 | Fermentation cylinder inoculation system convenient to sterilization and malleation supply liquid |
-
2022
- 2022-10-17 CN CN202211268230.7A patent/CN115678749A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100767569B1 (en) * | 2007-04-24 | 2007-10-17 | 김대환 | Fermentor |
| CN202369585U (en) * | 2011-12-27 | 2012-08-08 | 四川省申联生物科技有限责任公司 | Anaerobic inoculation tank |
| CN204385199U (en) * | 2014-12-31 | 2015-06-10 | 镇江东方生物工程设备技术有限责任公司 | A kind of inoculating device of fermentation tank |
| CN104911096A (en) * | 2015-06-10 | 2015-09-16 | 湖北五林中地农业科技有限公司 | Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique |
| CN114958567A (en) * | 2022-06-30 | 2022-08-30 | 江苏丰泽生物工程设备制造有限公司 | Sterile inoculation device for fermentation tank and use method thereof |
| CN218778943U (en) * | 2022-10-17 | 2023-03-31 | 安琪酶制剂(宜昌)有限公司 | Fermentation cylinder inoculation system convenient to sterilization and malleation supply liquid |
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