CN213866191U

CN213866191U - Fermentation system

Info

Publication number: CN213866191U
Application number: CN202022137260.7U
Authority: CN
Inventors: 潘璐璐; 倪鸣; 王庆波; 崔峰; 林川岸; 杨超; 吉礼兵
Original assignee: Qingdao Huada Zhizao Pratt & Whitney Technology Co ltd
Current assignee: Qingdao Huada Zhizao Pratt & Whitney Technology Co ltd
Priority date: 2020-09-25
Filing date: 2020-09-25
Publication date: 2021-08-03
Anticipated expiration: 2030-09-25

Abstract

The utility model provides a fermentation system, including fermentation cylinder, dona tub and four valves, four valves include first shut-off valve, second shut-off valve, third shut-off valve and fourth shut-off valve. The first cut-off valve is respectively connected with the third cut-off valve and the fifth cut-off valve through a first pipeline and a second pipeline, the other end of the third cut-off valve is connected with the fermentation tank, and the other end of the fifth cut-off valve is connected with the seed tank. The second pipeline is connected with a third branch, and the third branch is connected with a sixth block valve. The fermentation system provided by the utility model can sterilize on line, and the fermentation tank does not need to be opened during inoculation, thereby greatly reducing the risk of infecting mixed bacteria; the seed liquid (bacterial liquid) does not need to be placed in a container on the four valve groups, and the concentration and the activity of the seed liquid (bacterial liquid) are not influenced; the device has the advantages of no open fire operation, safety, reliability, simple structure and convenient use.

Description

Fermentation system

Technical Field

The utility model relates to a biological fermentation technical field especially relates to a fermentation system.

Background

Industrially, fermentation generally refers to a process for producing or producing certain products (products having cellular metabolites, also having bacterial cells, enzymes, etc.) by using microorganisms, and mainly includes anaerobic fermentation and aerobic fermentation. Anaerobic fermentation is mainly used for producing alcohol, lactic acid and the like, and aerobic fermentation is mainly used for producing antibiotics, amino acids, enzyme preparations and the like. In the fermentation process, the inoculation of thalli belongs to the initial step of the whole fermentation process, and the most important point of the inoculation step is to prevent bacterial contamination.

In the prior art, the internationally popular inoculation mode is that four valve banks are adopted: and after the shake flask culture is finished, pouring the bacterial liquid in the shake flask into a container on a four-valve set in an ultra-clean workbench or a biological safety cabinet, sealing the container, installing the four-valve set on a pipeline of a fermentation tank to be inoculated, sterilizing the fermentation tank, cooling, opening a valve between the container and the fermentation tank, and allowing the bacterial liquid to flow into the fermentation tank to finish inoculation. In addition, the common inoculation mode in domestic laboratories is flame inoculation, and the common inoculation mode in factories is flame laminar flow inoculation.

Among the above inoculation methods, the four-valve group inoculation method has the following disadvantages: the bacterial liquid must be placed in a container on the four valve banks for about 1 hour, so that the concentration and the activity of the bacterial liquid are influenced; the disadvantages of flame inoculation and flame laminar inoculation are: both of these are non-closed systems, present the risk of introducing infectious microbes, and present a certain risk for open flame operation.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model aims to provide a novel fermentation system with four valves, which can reduce the risk of infecting miscellaneous bacteria.

In order to achieve the above purpose, the technical scheme of the utility model is that: a fermentation system comprises a fermentation tank, a seed tank and four valve banks. The four valve groups comprise a first block valve, a second block valve, a third block valve and a fourth block valve; one end of the third cut-off valve is connected with the fermentation tank, and the other end of the third cut-off valve is connected with the first cut-off valve through a first pipeline. The first pipeline is connected with a first branch at a position close to the first block valve, the second block valve is connected with the first branch, the first pipeline is connected with a second branch at a position close to the third block valve, and the fourth block valve is connected with the second branch.

In some specific embodiments, the other end of the first block valve is further connected to a fifth block valve through a second pipeline, the other end of the fifth block valve is connected to the seed tank, the second pipeline is connected to a third branch, and the third branch is connected to a sixth block valve.

In some more specific embodiments, the second block valve is used as a steam inlet to sterilize the fermentation system.

In some more specific embodiments, the fourth block valve is used as a feed inlet to feed the fermentor.

In some specific embodiments, the material includes a bacteriostatic agent, which can inhibit the growth of bacteria in the material and prevent the fermentation tank from being contaminated.

In some more specific embodiments, the sixth block valve serves as a vapor outlet.

In some more specific embodiments, the seed tank is used to introduce seed solution into the fermentor via a pressure differential.

During sterilization, the third block valve and the fifth block valve are closed, the second block valve is opened, pure steam or industrial steam is introduced through the second block valve, the steam is subjected to high-temperature sterilization on the first pipeline and the second pipeline through the first block valve, the fourth block valve and the sixth block valve, the first branch, the second branch and the third branch are subjected to sterilization, and the steam can be discharged through the sixth block valve and the second block valve. And after the sterilization is finished, closing the second cut-off valve, the fourth cut-off valve and the sixth cut-off valve, opening the third cut-off valve, the first cut-off valve and the fifth cut-off valve, introducing the seed liquid in the seed tank into the fermentation tank through differential pressure, closing the third cut-off valve after the inoculation is finished, and repeating the sterilization step. After sterilization, the fourth block valve can be used as a feed inlet to feed the fermentor.

The fermentation system provided by the utility model can sterilize on line, and the fermentation tank does not need to be opened during inoculation, thereby greatly reducing the risk of infecting mixed bacteria; the seed liquid (bacterial liquid) does not need to be placed in a container on the four valve groups, and the concentration and the activity of the seed liquid (bacterial liquid) are not influenced; the device has the advantages of no open fire operation, safety, reliability, simple structure and convenient use.

Drawings

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

FIG. 1 is a schematic structural diagram of the fermentation system of the present invention.

Description of reference numerals:

fermentation tank 1

Seeding tank 2

First shut-off valve 3

Second shut-off valve 4

Third shut-off valve 5

Fourth block valve 6

First branch 7

Second branch 8

Fifth block valve 9

Sixth block valve 10

Third branch 11

First pipe 12

Second pipe 13

The following detailed description will further describe embodiments of the invention in conjunction with the above-described drawings.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which embodiments of the present invention belong. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of embodiments of the invention.

As shown in figure 1, the utility model provides a fermentation system, it includes fermentation cylinder 1, seedtank 2 and four valves. The four valve groups comprise a first block valve 3, a second block valve 4, a third block valve 5 and a fourth block valve 6; one end of the third cut-off valve 5 is connected with the fermentation tank 1, and the other end is connected with the first cut-off valve 3 through a first pipeline 12. The first pipe 12 is connected to a first branch 7 near the first block valve 3, and the second block valve 4 is connected to the first branch 7. The first duct 12 is connected to the second branch 8 close to the third shut-off valve 5, and the fourth shut-off valve 6 is connected to the second branch 8.

In this embodiment, the other end of the first block valve 3 is further connected to a fifth block valve 9 through a second pipeline 13, the other end of the fifth block valve 9 is connected to the seed tank 2, the second pipeline 13 is connected to a third branch 11, and the third branch 11 is connected to a sixth block valve 10.

In this embodiment, the second shut-off valve 4 is used as a steam inlet to sterilize the fermentation system.

In this example, the fourth block valve 6 is used as a feed inlet for feeding the fermenter 1.

In this embodiment, including the bacteriostat in the material, the bacteriostat can restrain the growth of bacterium in the material, prevents fermentation cylinder 1 from infecting the fungus.

In this embodiment, the sixth shut-off valve 10 may be used as a steam outlet.

In this embodiment, the seed liquid in the seed tank 2 is introduced into the fermentation tank 1 by a pressure difference.

During sterilization, the third block valve 5 and the fifth block valve 9 are closed, the second block valve 4 is opened, pure steam or industrial steam is introduced through the second block valve 4, the steam passes through the first block valve 3, the fourth block valve 6 and the sixth block valve 10, the first pipeline 12 and the third pipeline 13 are sterilized at high temperature, the first branch 7, the second branch 8 and the third branch 11 are sterilized, and the steam can be discharged through the sixth block valve 10 and the second block valve 4. And after the sterilization is finished, closing the second cut-off valve 4, the fourth cut-off valve 6 and the sixth cut-off valve 10, opening the third cut-off valve 5, the first cut-off valve 3 and the fifth cut-off valve 9, introducing the seed liquid in the seed tank 2 into the fermentation tank 1 through differential pressure, closing the third cut-off valve 5 after the inoculation is finished, and repeating the sterilization step. After sterilization, the fourth block valve 6 can also be used as a feed inlet for feeding the fermenter 1.

The above embodiments are only used to illustrate the technical solutions of the embodiments of the present invention and are not limited, and although the embodiments of the present invention have been described in detail with reference to the above preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions to the technical solutions of the embodiments of the present invention may be made without departing from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fermentation system comprises a fermentation tank, a seed tank and four valve banks, and is characterized in that the four valve banks comprise a first block valve, a second block valve, a third block valve and a fourth block valve; one end of the third cut-off valve is connected with the fermentation tank, and the other end of the third cut-off valve is connected with the first cut-off valve through a first pipeline; the first pipeline is connected with a first branch at a position close to the first cut-off valve, and the second cut-off valve is connected with the first branch; the first pipeline is connected with a second branch at a position close to the third block valve, and the fourth block valve is connected with the second branch;

the other end of the first block valve is further connected with a fifth block valve through a second pipeline, the other end of the fifth block valve is connected with the seed tank, the second pipeline is connected with a third branch, and the third branch is connected with a sixth block valve.