CN218579958U - Aseptic inoculation device is used in fermentation - Google Patents
Aseptic inoculation device is used in fermentation Download PDFInfo
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- CN218579958U CN218579958U CN202221915656.2U CN202221915656U CN218579958U CN 218579958 U CN218579958 U CN 218579958U CN 202221915656 U CN202221915656 U CN 202221915656U CN 218579958 U CN218579958 U CN 218579958U
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- fermentation
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- steam
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- 238000011081 inoculation Methods 0.000 title claims abstract description 46
- 238000000855 fermentation Methods 0.000 title claims abstract description 43
- 230000004151 fermentation Effects 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 abstract description 15
- 241000894006 Bacteria Species 0.000 abstract description 5
- 238000011109 contamination Methods 0.000 abstract description 4
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 abstract 1
- 235000013923 monosodium glutamate Nutrition 0.000 abstract 1
- 239000004223 monosodium glutamate Substances 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
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Abstract
The utility model relates to a fermentation is with aseptic inoculation device, include: an inoculation bottle, a connecting pipe for connecting the inoculation bottle to a fermentation tank, and a steam source for providing high-temperature steam; the connecting pipe is provided with a steam branch pipe used for being connected with a steam source and a first exhaust branch pipe connected with the outside; the inoculation bottle is provided with a mounting pipe connected with the connecting pipe, and the middle part of the mounting pipe is provided with a second exhaust branch pipe connected with the outside; the end parts of the mounting pipe and the connecting pipe are provided with fast-assembling interfaces which are matched with each other. The sterile inoculation device for fermentation is suitable for inoculation operation of fermentation industries such as antibiotics, monosodium glutamate, biological products and the like, has a simple structure, is convenient to operate, and has the following maximum advantages: the inoculation process is completely sealed, and the inoculation equipment and the channel are completely sterilized, so that the occurrence of bacteria contamination in the inoculation process is avoided.
Description
Technical Field
The utility model belongs to fermentation auxiliary assembly field, concretely relates to fermentation is with aseptic inoculation device.
Background
Fermentation refers to the process by which a person produces the microbial cells themselves, or direct or secondary metabolites, by virtue of the life activities of the microorganism under aerobic or anaerobic conditions. Fermentation is generally referred to as a process of decomposing organic substances by organisms. Fermentation is a biochemical reaction that humans have come into contact with earlier and is now widely used in the food industry, the biological and chemical industries. It is also a basic process of bioengineering, i.e. fermentation engineering. Many industrial products are currently produced by fermentation processes, such as many antibiotics, amino acids, vitamins and sugars.
In the fermentation process, if other microorganisms (also called mixed bacteria) except the production bacteria invade the fermentation liquor, the pure culture in the true sense is lost in the fermentation process. If the infectious microbes are infected to generate competition, the titer of the fermentation product is affected. Therefore, the occurrence of fermentation contamination is strictly controlled during the fermentation process.
In the fermentation process, inoculation is a necessary operation process, namely a process of adding production bacteria into a fermentation tank, and the fermentation tank needs to exchange substances with the outside, so that the process is the most easily infected, and therefore, if an aseptic inoculation device for fermentation can be developed, the occurrence of infection can be effectively avoided, and the method has important significance.
SUMMERY OF THE UTILITY MODEL
The utility model provides a fermentation is with aseptic inoculation device, when this fermentation is with aseptic inoculation device inoculates, the process is sealed completely, and inoculation equipment and passageway are thoroughly sterilized, have stopped the emergence of the in-process fungus contamination condition of inoculation
The technical scheme of the utility model as follows:
an aseptic inoculating device for fermentation, comprising: an inoculation bottle, a connecting pipe for connecting the inoculation bottle to a fermentation tank, and a steam source for providing high-temperature steam;
the connecting pipe is provided with a steam branch pipe used for being connected with a steam source and a first exhaust branch pipe connected with the outside;
the inoculation bottle is provided with a mounting pipe connected with the connecting pipe, and the middle part of the mounting pipe is provided with a second exhaust branch pipe connected with the outside;
the end parts of the mounting pipe and the connecting pipe are provided with fast-assembling interfaces which are matched with each other. Wherein, two fast-assembling interfaces link together through the mode of cup jointing, and simple structure can conveniently connect and unpack apart.
Preferably, a manual ball valve and a diaphragm valve are arranged on the first exhaust branch pipe;
the diaphragm valve is a manual diaphragm valve or a pneumatic diaphragm valve. And meanwhile, the two valves of the manual ball valve and the diaphragm valve are installed, so that the production accident caused by steam entering the tank due to valve leakage can be avoided.
Preferably, the steam branch pipe is provided with a manual diaphragm valve and a high-temperature stop valve. The valve rod of the adopted high-temperature stop valve has relatively short opening or closing stroke, has a very reliable cutting function and is very suitable for regulating the flow.
Preferably, the positions, close to the fermentation tank and the quick-assembly connector, on the connecting pipe are respectively provided with a manual diaphragm valve.
Preferably, a manual diaphragm valve is arranged on the second exhaust branch pipe;
and manual diaphragm valves are respectively arranged on the mounting pipe at positions close to the inoculation bottle and the quick-mounting connector.
Compared with the prior art, the beneficial effects of the utility model are embodied in:
adopt the utility model discloses a fermentation is with aseptic inoculation device simple structure, convenient operation to can guarantee that the inoculation process is sealed completely, inoculation equipment and passageway thoroughly sterilize, stopped the emergence of the inoculation in-process condition of dying fungus.
Drawings
Fig. 1 is a schematic structural diagram of the aseptic inoculation device for fermentation of the present invention, in which, 1: inoculating bottles; 2. 3, 4, 7 and 8 are all manual diaphragm valves; 5 and 6 are matched fast-assembling interfaces; 9: a high temperature stop valve; 10: a manual ball valve; 11 and 12: manual or pneumatic diaphragm valves.
Detailed Description
The structure of the sterile inoculating device for fermentation of the present invention is schematically illustrated in the attached drawings, and as can be seen from fig. 1, the sterile inoculating device for fermentation comprises: an inoculation bottle 1, a connecting pipe for connecting the inoculation bottle 1 to a fermentation tank, and a steam source for providing high-temperature steam;
the connecting pipe is provided with a steam branch pipe used for being connected with a steam source and a first exhaust branch pipe connected with the outside;
the inoculation bottle 1 is provided with a mounting pipe connected with the connecting pipe, and the middle part of the mounting pipe is provided with a second exhaust branch pipe connected with the outside;
the end parts of the mounting pipe and the connecting pipe are provided with a fast-assembling interface 5 and a fast-assembling interface 6 which are matched with each other;
wherein, a manual ball valve 10 and a diaphragm valve 11 are arranged on the first exhaust branch pipe;
the diaphragm valve is a manual diaphragm valve or a pneumatic diaphragm valve.
Wherein, the steam branch pipe is provided with a manual diaphragm valve 8 and a high-temperature stop valve 9.
Wherein, the positions of the connecting pipe close to the fermentation tank and the quick connector are respectively provided with a manual diaphragm valve which is respectively marked as a valve 12 and a valve 7.
Wherein, a manual diaphragm valve 3 is arranged on the second exhaust branch pipe;
and the positions, close to the inoculation bottle 1 and the quick-mounting connector, on the mounting pipe are respectively provided with a manual diaphragm valve which is respectively marked as a valve 2 and a valve 4.
The utility model discloses an inoculation device application method as follows:
(1) Before use, the parts 1-5 are wrapped with disinfectant paper, and all the inoculation bottles are placed in a disinfection kettle for disinfection.
(2) In a sterile environment, seed solution is potted into the inoculator and valve 5 and valve 4 are closed.
(3) Inoculation and disinfection: 5 and 6 are tightly connected through a quick-connection hoop, then a valve 7, a valve 4 and a valve 3 are opened, a valve 9, a valve 10 and a valve 11 are opened, finally a valve 8 is opened to introduce steam, and the inoculation pipeline is thoroughly sterilized by steam for 20-30 min.
(4) And (4) finishing disinfection: valves 7, 4, 3, 9, 10, 11 are closed in sequence, and finally steam valve 8 is closed, until the pipeline is cooled and ready for inoculation.
(5) Inoculation: and opening valves 2, 4, 7 and 12 in sequence to make the seed liquid naturally flow into the fermentation tank under the sterile environment. The inoculation is completed.
(6) And (3) completing inoculation: the valve 7 and valve 2 are closed, the quick connect clamps of 5 and 6 are disassembled, and the inoculator is removed. And opening valves 7, 10 and 11, and finally opening steam valves 8 and 9, and opening steam to purge residual seed liquid in the inoculation pipeline. And finally, closing all valves to finish cleaning the field.
Adopt the utility model discloses a fermentation is with aseptic inoculation device simple structure, convenient operation to stopped the emergence of the inoculation in-process contamination of bacteria condition.
Claims (5)
1. An aseptic inoculating device for fermentation, comprising: an inoculation bottle, a connecting pipe for connecting the inoculation bottle to a fermentation tank, and a steam source for providing high-temperature steam;
the connecting pipe is provided with a steam branch pipe used for being connected with a steam source and a first exhaust branch pipe connected with the outside;
the inoculation bottle is provided with a mounting pipe connected with the connecting pipe, and the middle part of the mounting pipe is provided with a second exhaust branch pipe connected with the outside;
the end parts of the mounting pipe and the connecting pipe are provided with fast-assembling interfaces which are matched with each other.
2. The aseptic inoculating device for fermentation of claim 1, wherein the first exhaust branch pipe is provided with a manual ball valve and a diaphragm valve;
the diaphragm valve is a manual diaphragm valve or a pneumatic diaphragm valve.
3. The aseptic inoculating device for fermentation of claim 1, wherein the steam branch pipe is provided with a manual diaphragm valve and a high temperature stop valve.
4. The aseptic inoculating device for fermentation of claim 1, wherein a manual diaphragm valve is provided on each connecting pipe at a position close to the fermentation tank and the quick connector.
5. The aseptic inoculating device for fermentation according to claim 1, wherein a manual diaphragm valve is arranged on the second exhaust branch pipe;
and manual diaphragm valves are respectively arranged on the mounting pipe at positions close to the inoculation bottle and the quick-mounting connector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221915656.2U CN218579958U (en) | 2022-07-22 | 2022-07-22 | Aseptic inoculation device is used in fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221915656.2U CN218579958U (en) | 2022-07-22 | 2022-07-22 | Aseptic inoculation device is used in fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218579958U true CN218579958U (en) | 2023-03-07 |
Family
ID=85360126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221915656.2U Active CN218579958U (en) | 2022-07-22 | 2022-07-22 | Aseptic inoculation device is used in fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218579958U (en) |
-
2022
- 2022-07-22 CN CN202221915656.2U patent/CN218579958U/en active Active
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