CN215712991U - Aseptic sampling device of microbial fermentation jar - Google Patents
Aseptic sampling device of microbial fermentation jar Download PDFInfo
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- CN215712991U CN215712991U CN202121965539.2U CN202121965539U CN215712991U CN 215712991 U CN215712991 U CN 215712991U CN 202121965539 U CN202121965539 U CN 202121965539U CN 215712991 U CN215712991 U CN 215712991U
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Abstract
The utility model discloses an aseptic sampling device of a microbial fermentation tank, which comprises an aseptic sampling system and an aseptic sampling bottle; the sterile sampling system consists of a steam inlet valve, a first sampling valve, a second sampling valve, a third sampling valve, a steam pipe, a sample outlet pipe and a first sampling chuck; the sterile sampling bottle consists of a second sampling chuck, an exhaust valve, a fourth sampling valve, a sampling pipeline, a sampling bottle, a bottle cap, an exhaust pipe and a breathing filter; the sterile sampling device has the advantages of simple structure, convenient operation and repeated use, can perform complex sterile sampling and rapid non-sterile sampling, can remarkably reduce the contamination risk in the sterile sampling process, and is worthy of popularization and application.
Description
Technical Field
The utility model relates to the technical field of microbial fermentation equipment, in particular to an aseptic sampling device of a microbial fermentation tank.
Background
The fermentation engineering is a process for producing metabolites or expression products required by human beings by culturing microorganisms or animal and plant cells on a large scale and utilizing a specific metabolic pathway or an expression system of the microorganisms or animal and plant cells. Fermentation engineering is an important component of modern biotechnology systems, plays a key role in the biotechnology industrialization process, and is widely applied to the biological manufacture of various products such as antibiotics, vitamins, amino acids, organic acids, enzyme preparations, monoclonal antibodies, vaccines, cytokines and the like. In the fermentation process, the culture solution in the fermentation tank is often required to be sampled, aseptic tracking detection is generally carried out by aseptic culture sampling, and physicochemical detection is carried out on indexes such as microorganism or cell morphology, concentration and product content by conventional detection sampling. In the fermentation production, generally, sampling is carried out at least once every day or multiple times a day, and the growth state of microorganisms or cells in the fermentation tank is judged through the sterile detection and metabolic parameter analysis of the samples, so that a basis is provided for the next fermentation parameter regulation and control.
Sterile culture sampling and conventional detection sampling both require to ensure that the sampling process is strict and aseptic, otherwise, the product in the fermentation tank is polluted by mixed bacteria, the fermentation is failed, and irretrievable huge economic loss is caused. At present, the mainstream sampling mode of the microbial fermentation tank is to weld an aseptic sampling valve at the side position of the bottom of the fermentation tank and arrange a special sampling pipeline on the sampling valve for sampling. This kind of sample mode can guarantee to take out the culture solution smoothly from the fermentation cylinder and can not cause the pollution to the culture solution in the fermentation cylinder, nevertheless because the follow-up aseptic safeguard measure that does not have of sampling pipe, when sampling pipe is connected with sample receiving bottle, the kneck exposes easily in external environment to the culture solution that takes out in making the fermentation cylinder receives ring surrounding environment microbial contamination easily, thereby brings the deviation or misjudgement for the aseptic trail detection of sample. The disposable aseptic sampler which is started in recent years can ensure that the sampled product is not contacted with the outside and is free from microbial contamination, and can carry out online cleaning and online sterilization, but the price is high, and the aseptic sampling unit can not be reused, so that the aseptic sampler is mainly used for aseptic sampling in the process of producing biological products by cell fermentation at present and is difficult to popularize and apply in microbial fermentation sampling.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides an aseptic sampling device of a microbial fermentation tank, which comprises a fermentation tank, wherein an aseptic sampling system and an aseptic sampling bottle are arranged on one side of the fermentation tank; the sterile sampling system consists of a steam inlet valve, a first sampling valve, a second sampling valve, a third sampling valve, a steam pipe, a sample outlet pipe and a first sampling chuck; the first sampling valve is fixed below the side position of the fermentation tank through an NA interface sterile flange, one end of the first sampling valve is connected with the steam pipeline and the steam inlet valve, and the other end of the first sampling valve is connected with the sample outlet pipe and the second sampling valve; the middle of the second sampling valve is provided with a switching pipeline which is connected with a third sampling valve, and the other end of the second sampling valve is connected with the first sampling chuck.
Furthermore, the sterile sampling bottle comprises a second sampling chuck, an exhaust valve, a fourth sampling valve, a sampling tube, a bottle body, a bottle cap, an exhaust pipe and a breathing filter; the top of the bottle body is provided with a bottle cap, the bottle cap is hermetically connected with an exhaust pipe and a sampling pipe, the air inlet of the exhaust pipe is arranged at the bottle mouth of the bottle body, and the air outlet is arranged outside the bottle cap of the bottle body; one end of the sampling tube is positioned in the bottle body of the bottle body, and the other end of the sampling tube is positioned outside the bottle cap of the bottle body; one end of the sampling tube, which is positioned outside the bottle cap, is connected with a fourth sampling valve; a switching pipeline is arranged in the middle of the fourth sampling valve and is connected with the steam exhaust valve on the switching pipeline, one end of the fourth sampling valve is connected with the sampling pipe outside the bottle cap, and the other end of the fourth sampling valve is connected with the second sampling chuck.
Furthermore, the first sampling valve is a sanitary T-shaped sterile sampling valve, the second sampling valve and the fourth sampling valve are L-shaped integrated diaphragm valves, and the third sampling valve and the air inlet valve are straight-through manual diaphragm valves.
Furthermore, the pore diameter of the micropores of the breathing filter is 0.05-0.22 um, and the breathing filter is connected with the air outlet of the exhaust pipe through a high-temperature-resistant silicon rubber pipe.
Furthermore, the first sampling chuck and the second sampling chuck are connected in a sealing mode through a clamping hoop and a backing ring.
Furthermore, the bottle body is marked with scale marks convenient for quantitative sampling.
Specifically, at first carry out online steam sterilization and autoclave sterilization respectively to aseptic sampling system and bottle, then open the fast-assembling blind flange on aseptic sampling bottle second sample chuck and the fast-assembling blind flange on the aseptic sampling valve system first sample chuck, utilize clamp and backing ring with the first sample chuck of sample and second sample chuck sealing connection, further carry out online steam sterilization to aseptic sampling device again, open sampling valve and aseptic sampling device's sampling passageway at last, can carry out aseptic sampling. Specifically, the aseptic sampling system can be independently subjected to online steam sterilization, and the sampling valve and the sampling passage are opened to perform open non-aseptic sampling.
The utility model has the beneficial effects that: the utility model provides an aseptic sampling device of a microbial fermentation tank, which comprises an aseptic sampling system and an aseptic sampling bottle, wherein the aseptic sampling system and the aseptic sampling bottle are connected through a first sampling chuck and a second sampling chuck, and a connecting pipeline can carry out online steam sterilization and can safely take out a sample which is not polluted by external microbes from the fermentation tank. In addition, this aseptic sampling device simple structure, convenient operation, repeatedly usable not only can carry out aseptic sampling, also can realize quick non-aseptic sampling, have very high popularization and application and worth in the fermentation trade.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
The reference numerals are explained below: 1. a steam inlet valve; 2. a steam pipe; 3. a first sampling valve; 4. a sample outlet pipe; 5. a second sampling valve; 6. a third sampling valve; 7. a first sampling chuck; 8. a second sampling chuck; 9. a steam exhaust valve; 10. a fourth sampling valve; 11. a sampling tube; 12. a bottle body; 13. a bottle cap; 14. an exhaust pipe; 15. a breathing filter.
Detailed Description
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The utility model will be further described with reference to the accompanying drawings in which:
as shown in fig. 1, an aseptic sampling device for a microbial fermentation tank comprises a fermentation tank, an aseptic sampling system and an aseptic sampling bottle, wherein the aseptic sampling system consists of a steam inlet valve 1, a first sampling valve 3, a second sampling valve 5, a third sampling valve 6, a steam pipe 2, a sample outlet pipe 4 and a first sampling chuck 7; the first sampling valve 3 is fixed below the side position of the fermentation tank through an NA interface sterile flange, one end of the first sampling valve 3 is connected with the steam pipe 2 and the steam inlet valve 1, and the other end of the first sampling valve 3 is connected with the sample outlet pipe 4 and the second sampling valve 5; a switching pipeline is arranged in the middle of the second sampling valve 5 and is connected with a third sampling valve 6 on the switching pipeline, and the other end of the second sampling valve 5 is connected with a first sampling chuck 7; the sterile sampling bottle comprises a second sampling chuck 8, an exhaust valve 9, a fourth sampling valve 10, a sampling tube 11, a bottle body 12, a bottle cap 13, an exhaust pipe 14 and a breathing filter 15; scale marks convenient for quantitative sampling are marked on the bottle body 12, and a bottle cap 13 is arranged at the top; the bottle cap 13 is hermetically connected with an exhaust pipe 14 and a sampling pipe 11, the exhaust pipe 14, the sampling pipe 11 and the bottle cap 13 are hermetically connected, an air inlet of the exhaust pipe 14 is arranged at the bottle mouth of the bottle body 12, an air outlet is arranged outside the bottle cap 13 of the bottle body 12, and the air outlet is connected with a breathing filter 15 through a high-temperature-resistant silicon rubber pipe; one end of the sampling tube 11 is positioned in the body of the bottle body 12, and the other end is positioned outside the bottle cap 13 of the bottle body 12; one end of the sampling tube 11 positioned outside the bottle cap 13 is connected with a fourth sampling valve 10; a switching pipeline is arranged in the middle of the fourth sampling valve 10, a steam exhaust valve 9 is connected on the switching pipeline, one end of the fourth sampling valve 10 is connected with a sampling pipe 11 outside a bottle cap 13, and the other end of the fourth sampling valve is connected with a second sampling chuck 8. The first sampling chuck 7 and the second sampling chuck 8 are connected in a sealing mode through a clamping hoop and a backing ring. The first sampling valve 3 is a sanitary T-shaped sterile sampling valve, the second sampling valve 5 and the fourth sampling valve 10 are L-shaped integrated diaphragm valves, and the third sampling valve 6 and the air inlet valve are straight-through manual diaphragm valves.
The working principle of the utility model is as follows:
sterilization before sampling: firstly, sealing a second sampling chuck 8 by using a fast-assembling blind plate, closing an exhaust valve 9 and a fourth sampling valve 10, screwing a bottle body cover 13, installing a breathing filter 15, and putting the whole bottle body into a sterilization pot for high-pressure steam sterilization; and then opening the steam inlet valve 1, the second sampling valve 5 and the third sampling valve 6, performing online steam sterilization on the sampling pipeline, and closing the valves after the sterilization is finished.
And (3) sterilization during sampling: during the use, dismantle the fast-assembling blind plate of second sample chuck 8 get off, be connected through clamp and sealing gasket ring with aseptic sampling system's first sample chuck 7, then open exhaust valve 9, reopen fermentation cylinder sampling system steam access, carry out steam sterilization to first sample chuck 7, second sample chuck 8 and corresponding pipeline, after the sterilization, close steam access and exhaust valve 9 to guarantee that all pipelines of sampling device, valve and interface all are in sterile condition.
When sampling, the first sampling valve 3, the second sampling valve 5 and the fourth sampling valve 10 are opened, and the fermentation liquid enters the bottle body 12 along the downstream of the sampling pipeline. After the sample is taken, the first sampling valve 3 and the fourth sampling valve 10 are closed, the steam inlet valve 1 and the steam outlet valve 9 are opened, and steam is used for blowing, cleaning and disinfecting the residual fermentation liquor in the steam pipe 2. And finally, detaching the second sampling chuck 8 for sampling, performing fast-assembly blind plate sealing, and integrally transferring the sterile sampling device containing the culture solution to a clean workbench or a biological safety cabinet for subsequent culture, such as coating a flat dish or inoculating a liquid culture medium, so as to accurately analyze whether the batch of fermentation pollutes the mixed bacteria. When non-aseptic sampling is required, only the aseptic sampling system needs to be sterilized independently, and the aseptic sampling bottle 12 does not need to be additionally arranged, so that the operation is simpler and more convenient.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed.
Claims (6)
1. The utility model provides an aseptic sampling device of microbial fermentation jar, includes the fermentation cylinder, its characterized in that: one side of the fermentation tank is provided with an aseptic sampling system and an aseptic sampling bottle; the sterile sampling system consists of a steam inlet valve (1), a first sampling valve (3), a second sampling valve (5), a third sampling valve (6), a steam pipe (2), a sample outlet pipe (4) and a first sampling chuck (7); the sterile sampling system is characterized in that a first sampling valve (3) is arranged on the fermentation tank, and the first sampling valve (3) is fixed below the side position of the fermentation tank through an NA interface sterile flange; one end of the first sampling valve (3) is connected with the steam pipeline (2) and the steam inlet valve (1), and the other end of the first sampling valve is connected with the sample outlet pipe (4) and the second sampling valve (5); the middle of the second sampling valve (5) is provided with a switching pipeline which is connected with a third sampling valve (6), and the other end of the second sampling valve (5) is connected with a first sampling chuck (7).
2. The aseptic sampling device of microbial fermentation tank of claim 1, wherein: the sterile sampling bottle comprises a second sampling chuck (8), a steam exhaust valve (9), a fourth sampling valve (10), a sampling tube (11), a bottle body (12), a bottle cap (13), an exhaust pipe (14) and a breathing filter (15); a bottle cap (13) is arranged at the top of the bottle body (12), and an exhaust pipe (14) and a sampling pipe (11) are hermetically connected to the bottle cap (13); the air inlet of the exhaust pipe (14) is arranged at the bottle mouth of the bottle body (12), and the air outlet is arranged outside the bottle cover (13) of the bottle body (12); one end of the sampling tube (11) is positioned in the bottle body (12) and the other end is positioned outside the bottle cap (13) of the bottle body (12); one end of the sampling tube (11) positioned outside the bottle cap (13) is connected with a fourth sampling valve (10); the middle of the fourth sampling valve (10) is provided with a switching pipeline and is connected with the steam exhaust valve (9) on the switching pipeline, one end of the fourth sampling valve (10) is connected with the outer sampling pipe (11) of the bottle cap (13), and the other end of the fourth sampling valve is connected with the second sampling chuck (8).
3. The aseptic sampling device of microbial fermentation tank of claim 2, wherein: the first sampling valve (3) is a sanitary T-shaped sterile sampling valve, the second sampling valve (5) and the fourth sampling valve (10) are L-shaped integrated diaphragm valves, and the third sampling valve (6) and the air inlet valve are straight-through manual diaphragm valves.
4. The aseptic sampling device of microbial fermentation tank of claim 2, wherein: the pore diameter of the micropores of the breathing filter (15) is 0.05-0.22 um, and the breathing filter is connected with the air outlet of the exhaust pipe (14) through a high-temperature-resistant silicon rubber pipe.
5. The aseptic sampling device of microbial fermentation tank of claim 2, wherein: the first sampling chuck (7) and the second sampling chuck (8) are connected in a sealing mode through a clamping hoop and a backing ring.
6. The aseptic sampling device of microbial fermentation tank of claim 2, wherein: the bottle body (12) is marked with scale marks convenient for quantitative sampling.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202121965539.2U CN215712991U (en) | 2021-08-20 | 2021-08-20 | Aseptic sampling device of microbial fermentation jar |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202121965539.2U CN215712991U (en) | 2021-08-20 | 2021-08-20 | Aseptic sampling device of microbial fermentation jar |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958582A (en) * | 2022-06-23 | 2022-08-30 | 赵玉玲 | Fermentation tank capable of detecting gas components in real time and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958582A (en) * | 2022-06-23 | 2022-08-30 | 赵玉玲 | Fermentation tank capable of detecting gas components in real time and use method thereof |
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