CN107063767A - A kind of sampler and fermentation tank sterile sampling method - Google Patents
A kind of sampler and fermentation tank sterile sampling method Download PDFInfo
- Publication number
- CN107063767A CN107063767A CN201710445494.8A CN201710445494A CN107063767A CN 107063767 A CN107063767 A CN 107063767A CN 201710445494 A CN201710445494 A CN 201710445494A CN 107063767 A CN107063767 A CN 107063767A
- Authority
- CN
- China
- Prior art keywords
- pipeline
- separation membrane
- membrane tube
- valve
- pressure gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005070 sampling Methods 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 title claims description 19
- 230000004151 fermentation Effects 0.000 title claims description 19
- 239000012528 membrane Substances 0.000 claims abstract description 80
- 238000000926 separation method Methods 0.000 claims abstract description 74
- 239000012530 fluid Substances 0.000 claims description 27
- 230000006641 stabilisation Effects 0.000 claims description 14
- 238000011105 stabilization Methods 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 8
- 239000000919 ceramic Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 4
- 230000000087 stabilizing effect Effects 0.000 claims 3
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 239000002052 molecular layer Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 11
- 238000011109 contamination Methods 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 5
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 19
- 238000001514 detection method Methods 0.000 description 14
- 238000000605 extraction Methods 0.000 description 12
- 238000011001 backwashing Methods 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 229920000620 organic polymer Polymers 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明公开了一种取样装置及发酵罐无菌取样方法，其中该装置包括高温高压气体发生器、分离膜管、支撑体和样品池，所述分离膜管固定在所述支撑体上，所述分离膜管的外壁周圈和外界直接接触，所述分离膜管的一端通过第一管路与所述高温高压气体发生器相连接，所述第一管路上设置有稳压稳流阀，所述分离膜管的另一端通过第二管路与所述样品池相连接，所述第二管路上设置有第一开关阀。本发明中采用设置高温高压气体发生器的方式，使得高温高压气体可以消除取样装置管路内残留的液滴，并防止管内壁生长微生物堵塞管路，避免了样品进入分离膜管内发生污染及堵塞的问题；同时在每次采样完成后，可以对分离膜管进行气体反向冲洗，避免了管路堵塞的问题。
The invention discloses a sampling device and a fermenter aseptic sampling method, wherein the device includes a high-temperature and high-pressure gas generator, a separation membrane tube, a support body and a sample pool, the separation membrane tube is fixed on the support body, and the The outer wall circumference of the separation membrane tube is in direct contact with the outside world, one end of the separation membrane tube is connected to the high-temperature and high-pressure gas generator through a first pipeline, and a pressure-stabilizing and flow-stabilizing valve is arranged on the first pipeline, The other end of the separation membrane tube is connected to the sample cell through a second pipeline, and a first switch valve is arranged on the second pipeline. In the present invention, the method of setting a high-temperature and high-pressure gas generator is adopted, so that the high-temperature and high-pressure gas can eliminate the residual liquid droplets in the pipeline of the sampling device, prevent the growth of microorganisms on the inner wall of the tube from blocking the pipeline, and avoid contamination and blockage of the sample entering the separation membrane tube At the same time, after each sampling is completed, the separation membrane tube can be backwashed with gas to avoid the problem of pipeline blockage.
Description
技术领域technical field
本发明涉及发酵过程取样检测技术领域，特别是涉及一种取样装置及发酵罐无菌取样方法。The invention relates to the technical field of sampling and detection in a fermentation process, in particular to a sampling device and a method for aseptically sampling a fermentation tank.
背景技术Background technique
生物反应器是利用酶或生物体(如微生物)所具有的生物功能，在体外进行生化反应的装置系统，它是一种生物功能模拟机(容器)，如发酵罐、酶或细胞反应器等，在食品与发酵(如酒类、氨基酸、有机酸)、医药(抗生素、干扰素)、环境(生物降解)、生物能源(纤维素乙醇、产油微藻、沼气)等生产和科研领域广泛应用。A bioreactor is a device system that uses the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator (container), such as a fermenter, enzyme or cell reactor, etc. , in food and fermentation (such as wine, amino acid, organic acid), medicine (antibiotics, interferon), environment (biodegradation), bioenergy (cellulosic ethanol, oil-producing microalgae, biogas) and other production and scientific research fields application.
要维持生物反应器的高效运行，即获得最大的生物转化率和最低的成本，需要对反应器中的生物过程进行控制，其控制的基础就是对反应器内各种参数进行检测。In order to maintain the high-efficiency operation of the bioreactor, that is, to obtain the maximum biotransformation rate and the lowest cost, it is necessary to control the biological process in the reactor, and the basis of the control is to detect various parameters in the reactor.
生物反应器的检测方式有离线检测和在线检测两种。离线检测是由人工取样，然后，将样品送到化验室或仪器室，通过专用仪器进行测定。在线检测是把检测仪器直接与反应器连接在一起或检测传感器探头插入反应器内进行检测。优点是取样和检测过程可连续、迅速、准确完成，能及时、准确、连续的反映反应器生化过程的动态变化，有利于生物反应过程的优化与控制。There are two detection methods of bioreactor: offline detection and online detection. Off-line detection is manual sampling, and then the samples are sent to the laboratory or instrument room for determination by special instruments. On-line detection is to connect the detection instrument directly with the reactor or insert the detection sensor probe into the reactor for detection. The advantage is that the sampling and detection process can be completed continuously, quickly and accurately, and can reflect the dynamic changes of the biochemical process of the reactor in a timely, accurate and continuous manner, which is beneficial to the optimization and control of the biological reaction process.
在线检测系统一般包含2个部分：(1)分析检测技术和设备(检测装置)；(2)能将检测装置和反应器内部介质相结合的装置即取样装置。The online detection system generally includes two parts: (1) analysis and detection technology and equipment (detection device); (2) a device that can combine the detection device with the internal medium of the reactor, that is, the sampling device.
现有技术中的取样装置主要包括两类，其中，一类是由管道和阀门组成，一类是分离膜过滤。管道阀门组成的取样装置不足之处在于：取出的是反应器内原样，含有菌体、原料粗渣等，往往需经过过滤处理后才能进入仪器测定；为避免取样过程的污染，必须需要配置灭菌装置，如蒸汽、紫外灯等。对于分离膜过滤取样，由于膜的孔径阻止了菌体的通过，避免了外源污染，不足之处是液体中的杂质容易造成分离膜网孔的阻塞，过滤稳定性差，需要经常清洗或更换。The sampling devices in the prior art mainly include two types, one of which is composed of pipelines and valves, and the other is separation membrane filtration. The disadvantage of the sampling device composed of pipeline valves is that what is taken out is the original sample in the reactor, containing bacteria, raw material slag, etc., which often need to be filtered before entering the instrument for measurement; Sterilization devices, such as steam, ultraviolet lamps, etc. For separation membrane filtration sampling, because the pore size of the membrane prevents the passage of bacteria and avoids external pollution, the disadvantage is that impurities in the liquid can easily cause blockage of the separation membrane mesh, poor filtration stability, and need to be cleaned or replaced frequently.
发明内容Contents of the invention
本发明的目的是提供一种取样效率高、取样质量好、使用安全方便的取样装置及发酵罐无菌取样方法，以解决现有发酵罐无菌取样装置及取样方法装置中分离膜网孔易阻塞、易污染导致取样样品质量差、取样效率低的问题。The purpose of the present invention is to provide a sampling device and a fermenter aseptic sampling method with high sampling efficiency, good sampling quality, safe and convenient use, so as to solve the problem of the separation membrane mesh in the existing fermenter aseptic sampling device and sampling method device. Blockage and easy contamination lead to poor sample quality and low sampling efficiency.
为实现上述目的，本发明提供了如下方案：本发明提供一种取样装置，包括高温高压气体发生器、分离膜管、支撑体和样品池，所述分离膜管固定在所述支撑体上，所述分离膜管的外壁周圈和外界直接接触，所述分离膜管的一端通过第一管路与所述高温高压气体发生器相连接，所述第一管路上设置有稳压稳流阀，所述分离膜管的另一端通过第二管路与所述样品池相连接，所述第二管路上设置有第一开关阀。In order to achieve the above object, the present invention provides the following scheme: the present invention provides a sampling device, comprising a high temperature and high pressure gas generator, a separation membrane tube, a support body and a sample cell, the separation membrane tube is fixed on the support body, The circumference of the outer wall of the separation membrane tube is in direct contact with the outside world, and one end of the separation membrane tube is connected to the high-temperature and high-pressure gas generator through a first pipeline, and a pressure-stabilizing and flow-stabilizing valve is provided on the first pipeline , the other end of the separation membrane tube is connected to the sample cell through a second pipeline, and a first switch valve is arranged on the second pipeline.
优选的，所述支撑体上设置有流体通道，所述分离膜管的两端分别与相应的所述流体通道相连接，所述流体通道分别与所述第一管路和所述第二管路相连接。Preferably, the support body is provided with a fluid channel, and the two ends of the separation membrane tube are respectively connected to the corresponding fluid channels, and the fluid channels are respectively connected to the first pipeline and the second tube. Road connected.
优选的，所述第二管路上还设置有第二开关阀，所述第二开关阀靠近其中一个所述流体通道。Preferably, a second on-off valve is further arranged on the second pipeline, and the second on-off valve is close to one of the fluid passages.
优选的，所述分离膜管的两端与所述流体通道的连接处设置有密封结构。Preferably, a sealing structure is provided at the connection between the two ends of the separation membrane tube and the fluid channel.
优选的，所述分离膜管的材质为多孔陶瓷或有机高分子膜；优选的，所述多孔陶瓷或有机高分子膜的孔径在0.1-0.2μm。Preferably, the material of the separation membrane tube is porous ceramic or organic polymer membrane; preferably, the pore diameter of the porous ceramic or organic polymer membrane is 0.1-0.2 μm.
优选的，所述第二管路上还设置有第二开关阀，所述第二开关阀靠近所述分离膜管。Preferably, a second on-off valve is further arranged on the second pipeline, and the second on-off valve is close to the separation membrane tube.
优选的，所述取样装置还包括抽取装置，所述抽取装置通过第三管路与所述第二管路相连接，所述第三管路与所述第二管路的连接处位于所述第一开关阀与所述分离膜管之间。Preferably, the sampling device further includes an extraction device, the extraction device is connected to the second pipeline through a third pipeline, and the connection between the third pipeline and the second pipeline is located in the Between the first switch valve and the separation membrane tube.
优选的，所述第三管路上设置有第三开关阀。Preferably, a third on-off valve is arranged on the third pipeline.
本发明中应用上述取样装置，还提供了一种发酵罐无菌取样方法，其特征在于，包括如下步骤：Application of above-mentioned sampling device in the present invention also provides a kind of fermentation tank aseptic sampling method, it is characterized in that, comprises the steps:
将取样装置中的支撑体和分离膜管插入发酵罐；Insert the support body and the separation membrane tube in the sampling device into the fermenter;
所述取样装置中的稳压稳流阀、第一开关阀、第二开关阀和第三开关阀开启，并通过高温高压气体发生器向所述取样装置中通入高温高压气体进行灭菌；The pressure and flow stabilization valve, the first on-off valve, the second on-off valve and the third on-off valve in the sampling device are opened, and a high-temperature and high-pressure gas is passed into the sampling device through a high-temperature and high-pressure gas generator for sterilization;
灭菌完成后关闭所述高温高压气体发生器，并将所述稳压稳流阀和所述第一开关阀关闭，所述第二开关阀和所述第三开关阀开启，所述取样装置中的抽取装置启动，所述抽取装置将所述分离膜管外部的发酵液，抽入所述分离膜管的内部；After the sterilization is completed, close the high-temperature and high-pressure gas generator, close the pressure-stabilizing and flow-stabilizing valve and the first on-off valve, open the second on-off valve and the third on-off valve, and open the sampling device The extracting device in the pump is started, and the extracting device draws the fermentation liquid outside the separation membrane tube into the inside of the separation membrane tube;
所述抽取装置抽取完成后关闭所述抽取装置，并将所述稳压稳流阀、所述第一开关阀和所述第二开关阀开启，将所述第三开关阀关闭，所述高温高压气体发生器启动，所述高温高压气体发生器产生的高温高压气体推动所述分离膜管内部的发酵液进入所述取样装置中的样品池。After the extraction of the extraction device is completed, the extraction device is closed, the pressure and flow stabilization valve, the first on-off valve and the second on-off valve are opened, the third on-off valve is closed, and the high temperature The high-pressure gas generator is started, and the high-temperature and high-pressure gas generated by the high-temperature and high-pressure gas generator pushes the fermentation liquid inside the separation membrane tube into the sample pool in the sampling device.
优选的，取样完成后，保持所述稳压稳流阀开启，并将所述第一开关阀、所述第二开关阀和所述第三开关阀关闭，所述高温高压气体发生器产生的高温高压气体对所述分离膜管进行反冲洗。Preferably, after the sampling is completed, keep the pressure and flow stabilization valve open, and close the first on-off valve, the second on-off valve and the third on-off valve, and the high-temperature and high-pressure gas generator generates The high-temperature and high-pressure gas backwashes the separation membrane tube.
本发明相对于现有技术取得了以下有益效果：Compared with the prior art, the present invention has achieved the following beneficial effects:
1、本发明中采用设置高温高压气体发生器的方式，使得高温高压气体可以消除取样装置管路内残留的液滴，避免了分离膜管外发酵液进入管内后与残留液体融合后产生浓度误差的问题，进而保证了管内样品浓度和管外发酵液浓度的一致性，同时高温高压气体也可以防止管道内产生微生物，避免了发酵液样品进入分离膜管内发生污染的问题，也了防止了管内壁生长微生物导致分离膜管内部堵塞的问题。1. In the present invention, the method of setting a high-temperature and high-pressure gas generator is adopted, so that the high-temperature and high-pressure gas can eliminate the residual liquid droplets in the pipeline of the sampling device, and avoid the concentration error after the fermentation liquid outside the separation membrane tube enters the tube and merges with the residual liquid In order to ensure the consistency of the concentration of the sample in the tube and the concentration of the fermentation liquid outside the tube, at the same time, the high temperature and high pressure gas can also prevent the generation of microorganisms in the pipeline, avoiding the problem of contamination of the fermentation liquid sample entering the separation membrane tube, and also preventing the contamination of the tube. The wall growth microorganisms cause the problem of clogging inside the separation membrane tube.
2、本发明中分离膜管的材质为多孔陶瓷或有机高分子膜，能耐高温灭菌，孔径在0.1-0.2μm之间，微生物不能通过，能有效防止取样样品中存在菌体，取出的样品(发酵液)没有杂质，不需过滤处理就适用于多种分析仪器直接测定。2. The material of the separation membrane tube in the present invention is porous ceramic or organic polymer membrane, which can withstand high temperature sterilization. The pore size is between 0.1-0.2 μm, and microorganisms cannot pass through, which can effectively prevent the presence of bacteria in the sampling sample. The sample taken out (Fermentation broth) has no impurities and is suitable for direct determination by various analytical instruments without filtering.
3、本发明中在每次采样过程中或者完成后，可以对分离膜管进行高温高压气体的反向冲洗，清洗掉附着在管路外壁的菌体，保持管路外壁的长期清洁，避免了样品流通的生物污染堵塞，提高了取样效率。3. In the present invention, during or after each sampling process, the separation membrane tube can be backwashed with high-temperature and high-pressure gas to clean away the bacteria attached to the outer wall of the pipeline, and keep the outer wall of the pipeline clean for a long time, avoiding the Bio-contamination blockage of sample flow improves sampling efficiency.
4、本发明中在进行取样工作之前，先通过高温高压气体发生器产生的高温高压气体对整个取样装置进行灭菌处理，保证取样装置本身不存在污染源，避免了由于取样装置的污染物导致整个发酵罐被污染的问题，提高了取样过程的安全性能。4. In the present invention, before sampling work, the entire sampling device is sterilized by the high-temperature and high-pressure gas generated by the high-temperature and high-pressure gas generator to ensure that there is no pollution source in the sampling device itself, and avoid the contamination of the entire sampling device due to pollutants in the sampling device. The problem of contamination of the fermenter improves the safety performance of the sampling process.
5、由于使用取样装置在发酵罐取出的样品中含有丰富的营养物，易在流通管道(包括分离膜管、第一管路、第二管路、流体通道等)中产生残留，引起流通管道的污染，本发明中通过高温高压气体发生器产生的高温高压气体对整个流通管道进行灭菌，可避免流通管道污染造成的管道堵塞，提高了管道流通的稳定性。5. Due to the rich nutrients in the samples taken out of the fermenter using the sampling device, it is easy to produce residues in the circulation pipelines (including separation membrane tubes, first pipelines, second pipelines, fluid channels, etc.), causing circulation pipelines In the present invention, the high-temperature and high-pressure gas generated by the high-temperature and high-pressure gas generator sterilizes the entire circulation pipeline, which can avoid the pipeline blockage caused by the pollution of the circulation pipeline and improve the stability of the pipeline circulation.
附图说明Description of drawings
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without paying creative labor.
图1为本发明取样装置的整体结构示意图；Fig. 1 is the overall structure schematic diagram of sampling device of the present invention;
其中，1-高温高压气体发生器、2-分离膜管、3-支撑体、4-样品池、5-第一管路、6-稳压稳流阀、7-第二管路、8-第一开关阀、9-流体通道、10-第二开关阀、11-密封结构、12-抽取装置、13-第三开关阀、14-第三管路。Among them, 1-high temperature and high pressure gas generator, 2-separation membrane tube, 3-support body, 4-sample pool, 5-first pipeline, 6-stabilizing pressure and flow valve, 7-second pipeline, 8- First switch valve, 9-fluid channel, 10-second switch valve, 11-sealing structure, 12-extraction device, 13-third switch valve, 14-third pipeline.
具体实施方式detailed description
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
本发明的目的是提供一种取样效率高、取样质量好、使用方便的取样装置及发酵罐无菌取样方法，以解决现有发酵罐无菌取样装置及取样方法装置中分离膜网孔易阻塞、易污染导致取样样品质量差、取样效率低的问题。The purpose of the present invention is to provide a sampling device with high sampling efficiency, good sampling quality and convenient use and aseptic sampling method for fermentation tanks, so as to solve the problem of easy blockage of separation membrane mesh in existing aseptic sampling devices for fermentation tanks and sampling method devices , Poor sample quality and low sampling efficiency due to easy pollution.
为使本发明的上述目的、特征和优点能够更加明显易懂，下面结合附图和具体实施方式对本发明作进一步详细的说明。In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
如图1所示，本发明提供一种取样装置，包括高温高压气体发生器1、分离膜管2、支撑体3和样品池4，所述分离膜管2固定在所述支撑体3上，所述分离膜管2的外壁周圈和外界直接接触，所述分离膜管2的一端通过第一管路5与所述高温高压气体发生器1相连接，所述第一管路5上设置有稳压稳流阀6，所述分离膜管2的另一端通过第二管路7与所述样品池4相连接，所述第二管路7上设置有第一开关阀8。As shown in Figure 1, the present invention provides a kind of sampling device, comprises high temperature and high pressure gas generator 1, separation membrane tube 2, support body 3 and sample cell 4, and described separation membrane tube 2 is fixed on the described support body 3, The outer wall circumference of the separation membrane tube 2 is in direct contact with the outside world, and one end of the separation membrane tube 2 is connected to the high-temperature and high-pressure gas generator 1 through a first pipeline 5, and the first pipeline 5 is provided with There is a pressure and flow stabilization valve 6, and the other end of the separation membrane tube 2 is connected to the sample cell 4 through a second pipeline 7, and a first on-off valve 8 is arranged on the second pipeline 7.
本发明取样装置在工作过程中，高温高压气体发生器1中产生高温高压气体，高温高压气体经过稳压稳流阀6的控制稳定的输送到取样装置的管路系统中，在取样装置进行取样工作之前高温高压气体可以对管路系统进行灭菌，在进行取样工作时高温高压气体推动由分离膜管2渗透入管路内部的样品，使得样品进入样品池4中，等待使用；在取样工作完成之后，关闭设置在第二管路7上的第一开关阀8，使得高温高压气体在分离膜管2的孔隙中流出，形成气体反冲洗，清洗掉附着在分离膜管2管路外壁的菌体，保持管路外壁的长期清洁。其中，支撑体3是由有机材料棒加工而成的圆柱体结构，直径为1.0-2.5cm，分离膜管2的内径径为0.5-2.0mm。During the working process of the sampling device of the present invention, high-temperature and high-pressure gas is generated in the high-temperature and high-pressure gas generator 1, and the high-temperature and high-pressure gas is stably transported to the pipeline system of the sampling device through the control of the pressure-stabilizing and flow-stabilizing valve 6, and the sampling is performed in the sampling device Before work, the high-temperature and high-pressure gas can sterilize the pipeline system. During the sampling work, the high-temperature and high-pressure gas pushes the sample infiltrated into the pipeline by the separation membrane tube 2, so that the sample enters the sample pool 4 and waits for use; After that, close the first switch valve 8 provided on the second pipeline 7, so that the high-temperature and high-pressure gas flows out in the pores of the separation membrane tube 2, forming a gas backwash, and cleaning away the bacteria attached to the outer wall of the separation membrane tube 2 pipeline. Body, keep the outer wall of the pipeline clean for a long time. Wherein, the support body 3 is a cylindrical structure processed from organic material rods, with a diameter of 1.0-2.5 cm, and the inner diameter of the separation membrane tube 2 is 0.5-2.0 mm.
由于高温高压气体在管路中运动时产生很强的作用力(如对管壁冲击力)，容易对管路产生损坏，或者对管路连接处(如第一管路5与分离膜管2的连接处，若第一管路5在高温高压气体的作用下产生强烈震动或抖动，将破坏连接处的结构强度，影响使用寿命和密封性)的密封性能产生影响，为了保证管路的使用寿命和密封性，本发明中在所述支撑体3上设置有流体通道9，所述分离膜管2的两端分别与相应的所述流体通道9相连接，所述流体通道9分别与所述第一管路5和所述第二管路7相连接。本发明中采用在支撑体3上设置流体通道9的方式，使得分离膜管2的两端通过相应的流体通道9分别第一管路5和第二管路7相连接，避免了分离膜管2直接与第一管路5和第二管路7相连接，保证管路(包括分离膜管2、第一管路5、第二管路7等)的使用寿命和密封性，其中，流体通道9呈U型，即U型扩散管道，管道内径为0.5-2.0mm。Since the high-temperature and high-pressure gas generates a strong force (such as impact on the pipe wall) when it moves in the pipeline, it is easy to damage the pipeline, or damage the pipeline connection (such as the first pipeline 5 and the separation membrane tube 2). If the first pipeline 5 vibrates or vibrates strongly under the action of high-temperature and high-pressure gas, it will destroy the structural strength of the connection, affect the service life and sealing performance), in order to ensure the use of the pipeline life and tightness, in the present invention, a fluid channel 9 is arranged on the support body 3, and the two ends of the separation membrane tube 2 are respectively connected with the corresponding fluid channel 9, and the fluid channel 9 is connected with the corresponding fluid channel 9 respectively. The first pipeline 5 and the second pipeline 7 are connected. In the present invention, the fluid channel 9 is arranged on the support body 3, so that the two ends of the separation membrane tube 2 are connected with the first pipeline 5 and the second pipeline 7 through the corresponding fluid channels 9, avoiding the separation membrane tube 2. It is directly connected with the first pipeline 5 and the second pipeline 7 to ensure the service life and tightness of the pipelines (including the separation membrane tube 2, the first pipeline 5, the second pipeline 7, etc.), wherein the fluid The channel 9 is U-shaped, that is, a U-shaped diffusion pipe, and the inner diameter of the pipe is 0.5-2.0mm.
为了避免反冲洗过程中高温高压气体对第二管路7产生的不良影响，如气体冲击导致第二管路7使用寿命低，又或者由于过长的第二管路7导致反冲洗气体压力不足的问题，本发明中在所述第二管路7上还设置有第二开关阀7，所述第二开关阀7靠近其中一个所述流体通道9(此流体通道9为与第二管路7相连接的流体通道9)；在进行反冲洗过程中关闭第二开关阀7，缩短高温高压气体在第二管路7中的流动距离。In order to avoid the adverse effects of high temperature and high pressure gas on the second pipeline 7 during the backwashing process, such as the gas shock causing the second pipeline 7 to have a low service life, or the pressure of the backwashing gas is insufficient due to the excessively long second pipeline 7 problem, in the present invention, a second on-off valve 7 is also provided on the second pipeline 7, and the second on-off valve 7 is close to one of the fluid channels 9 (this fluid channel 9 is connected to the second pipeline 7) connected to the fluid channel 9); in the process of backwashing, the second switch valve 7 is closed to shorten the flow distance of the high-temperature and high-pressure gas in the second pipeline 7.
本发明中所述分离膜管2的两端与所述流体通道9的连接处设置有密封结构11，保证分离膜管2与流体通道9的密封性能，其中，密封结构11可以为密封圈。In the present invention, a sealing structure 11 is provided at the connection between the two ends of the separation membrane tube 2 and the fluid channel 9 to ensure the sealing performance of the separation membrane tube 2 and the fluid channel 9, wherein the sealing structure 11 can be a sealing ring.
本发明中所述分离膜管2的材质为多孔陶瓷或有机高分子膜；优选的，所述多孔陶瓷或有机高分子膜的孔径在0.1-0.2μm。The material of the separation membrane tube 2 in the present invention is porous ceramic or organic polymer membrane; preferably, the pore diameter of the porous ceramic or organic polymer membrane is 0.1-0.2 μm.
为了避免反冲洗过程中高温高压气体对第二管路7产生的不良影响，如气体冲击导致第二管路7使用寿命低，又或者由于过长的第二管路7导致反冲洗气体压力不足的问题，本发明中在所述第二管路7上还设置有第二开关阀7，所述第二开关阀7靠近所述分离膜管2(即靠近第二管路7与分离膜管2的连接处)，在进行反冲洗过程中关闭第二开关阀7，缩短高温高压气体在第二管路7中的流动距离。In order to avoid the adverse effects of high temperature and high pressure gas on the second pipeline 7 during the backwashing process, such as the gas shock causing the second pipeline 7 to have a low service life, or the pressure of the backwashing gas is insufficient due to the excessively long second pipeline 7 problem, in the present invention, a second on-off valve 7 is also provided on the second pipeline 7, and the second on-off valve 7 is close to the separation membrane tube 2 (that is, close to the second pipeline 7 and the separation membrane tube 2), close the second on-off valve 7 during the backwashing process to shorten the flow distance of the high-temperature and high-pressure gas in the second pipeline 7.
为了提高取样装置的取样效率，本发明中所述取样装置还包括抽取装置12，所述抽取装置12通过第三管路14与所述第二管路7相连接，所述第三管路14与所述第二管路7的连接处位于所述第一开关阀8与所述分离膜管2之间，在取样过程中抽取装置12抽取管路系统(包括分离膜管2、第一管路5、第二管路7、第三管路14等)内部的气体，使得管路系统内部形成负压，进而使得外界流体在负压的作用下快速的进入管路系统内部，提高了取样效率。In order to improve the sampling efficiency of the sampling device, the sampling device in the present invention also includes an extraction device 12, and the extraction device 12 is connected to the second pipeline 7 through a third pipeline 14, and the third pipeline 14 The connection with the second pipeline 7 is located between the first switch valve 8 and the separation membrane tube 2, and the extraction device 12 extracts the pipeline system (comprising the separation membrane tube 2, the first tube 2) during the sampling process. 5, the second pipeline 7, the third pipeline 14, etc.) to form a negative pressure inside the pipeline system, and then make the external fluid quickly enter the pipeline system under the action of negative pressure, which improves the sampling efficiency. efficiency.
本发明中所述第三管路14上设置有第三开关阀13，实现对第三管路14的贯通或者关闭。In the present invention, the third on-off valve 13 is provided on the third pipeline 14 to realize the connection or closure of the third pipeline 14 .
本发明中应用上述取样装置，还提供了一种发酵罐无菌取样方法，包括如下步骤：Apply above-mentioned sampling device among the present invention, also provide a kind of aseptic sampling method of fermentation tank, comprise the steps:
将取样装置中的支撑体3和分离膜管2插入发酵罐；Insert the support body 3 and the separation membrane tube 2 in the sampling device into the fermenter;
所述取样装置中的稳压稳流阀6、第一开关阀8、第二开关阀7和第三开关阀13开启，并通过高温高压气体发生器1向所述取样装置中通入高温高压气体进行灭菌；The pressure and flow stabilization valve 6, the first on-off valve 8, the second on-off valve 7 and the third on-off valve 13 in the sampling device are opened, and a high-temperature and high-pressure gas is introduced into the sampling device through the high-temperature and high-pressure gas generator 1. gas for sterilization;
灭菌完成后关闭所述高温高压气体发生器1，并将所述稳压稳流阀6和所述第一开关阀8关闭，所述第二开关阀7和所述第三开关阀13开启，所述取样装置中的抽取装置12启动，所述抽取装置12将所述分离膜管2外部的发酵液，抽入所述分离膜管2的内部；After the sterilization is completed, close the high temperature and high pressure gas generator 1, close the pressure and flow stabilization valve 6 and the first switch valve 8, and open the second switch valve 7 and the third switch valve 13 , the extracting device 12 in the sampling device starts, and the extracting device 12 draws the fermentation liquid outside the separation membrane tube 2 into the inside of the separation membrane tube 2;
所述抽取装置12抽取完成后关闭所述抽取装置12，并将所述稳压稳流阀6、所述第一开关阀8和所述第二开关阀7开启，将所述第三开关阀13关闭，所述高温高压气体发生器1启动，所述高温高压气体发生器1产生的高温高压气体推动所述分离膜管2内部的发酵液进入所述取样装置中的样品池4。After the extraction of the extraction device 12 is completed, the extraction device 12 is closed, and the pressure and flow stabilization valve 6, the first on-off valve 8 and the second on-off valve 7 are opened, and the third on-off valve is opened. 13 is closed, the high temperature and high pressure gas generator 1 is started, and the high temperature and high pressure gas generated by the high temperature and high pressure gas generator 1 pushes the fermentation liquid inside the separation membrane tube 2 into the sample pool 4 in the sampling device.
更进一步的，在取样完成后，保持所述稳压稳流阀6开启，并将所述第一开关阀8、所述第二开关阀7和所述第三开关阀13关闭，所述高温高压气体发生器1产生的高温高压气体对所述分离膜管2进行反冲洗。Furthermore, after the sampling is completed, keep the pressure and flow stabilization valve 6 open, and close the first on-off valve 8, the second on-off valve 7 and the third on-off valve 13, the high temperature The high-temperature and high-pressure gas generated by the high-pressure gas generator 1 backwashes the separation membrane tube 2 .
本发明中应用了具体个例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想；同时，对于本领域的一般技术人员，依据本发明的思想，在具体实施方式及应用范围上均会有改变之处。综上所述，本说明书内容不应理解为对本发明的限制。In the present invention, specific examples have been used to illustrate the principle and implementation of the present invention. The description of the above embodiments is only used to help understand the method and core idea of the present invention; meanwhile, for those of ordinary skill in the art, according to the present invention The idea of the invention will have changes in the specific implementation and scope of application. In summary, the contents of this specification should not be construed as limiting the present invention.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710445494.8A CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710445494.8A CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107063767A true CN107063767A (en) | 2017-08-18 |
| CN107063767B CN107063767B (en) | 2019-05-21 |
Family
ID=59595518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710445494.8A Expired - Fee Related CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107063767B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107621550A (en) * | 2017-11-08 | 2018-01-23 | 苏州市苏测检测技术有限公司 | Food elution detection integration apparatus |
| CN109813575A (en) * | 2019-02-22 | 2019-05-28 | 湖南莱博赛医用机器人有限公司 | A kind of device and method automatically extracting liquid sample |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09126961A (en) * | 1995-10-31 | 1997-05-16 | Oji Paper Co Ltd | Concentration measurement method |
| CN201971832U (en) * | 2011-02-23 | 2011-09-14 | 镇江日泰生物工程设备有限公司 | Sampling device for fermentation tank |
| CN102279118A (en) * | 2011-05-05 | 2011-12-14 | 山东省科学院生物研究所 | Concentration gradient diffusion sampler for bioreactor on-line testing system |
| CN102928256A (en) * | 2012-11-20 | 2013-02-13 | 北京雪迪龙科技股份有限公司 | Water quality sampling device and water quality monitoring system employing same |
| CN203396611U (en) * | 2013-08-08 | 2014-01-15 | 中国石油化工股份有限公司 | Automatic backwash filter sampling device |
| CN103698157A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Special sampling device for fermentation tank |
| CN103789205A (en) * | 2014-03-12 | 2014-05-14 | 河北工程大学 | Solar microbial fermentation device |
| CN104677675A (en) * | 2013-12-03 | 2015-06-03 | 华东理工大学 | Sampling device and biological reaction device with sampling device |
-
2017
- 2017-06-14 CN CN201710445494.8A patent/CN107063767B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09126961A (en) * | 1995-10-31 | 1997-05-16 | Oji Paper Co Ltd | Concentration measurement method |
| CN201971832U (en) * | 2011-02-23 | 2011-09-14 | 镇江日泰生物工程设备有限公司 | Sampling device for fermentation tank |
| CN102279118A (en) * | 2011-05-05 | 2011-12-14 | 山东省科学院生物研究所 | Concentration gradient diffusion sampler for bioreactor on-line testing system |
| CN102928256A (en) * | 2012-11-20 | 2013-02-13 | 北京雪迪龙科技股份有限公司 | Water quality sampling device and water quality monitoring system employing same |
| CN203396611U (en) * | 2013-08-08 | 2014-01-15 | 中国石油化工股份有限公司 | Automatic backwash filter sampling device |
| CN104677675A (en) * | 2013-12-03 | 2015-06-03 | 华东理工大学 | Sampling device and biological reaction device with sampling device |
| CN103698157A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Special sampling device for fermentation tank |
| CN103789205A (en) * | 2014-03-12 | 2014-05-14 | 河北工程大学 | Solar microbial fermentation device |
Non-Patent Citations (1)
| Title |
|---|
| 朱思荣等: "发酵过程生化指标检测的自动取样设计", 《山东科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107621550A (en) * | 2017-11-08 | 2018-01-23 | 苏州市苏测检测技术有限公司 | Food elution detection integration apparatus |
| CN107621550B (en) * | 2017-11-08 | 2024-05-07 | 济南佳宝乳业有限公司 | Food elution detection integrated equipment |
| CN109813575A (en) * | 2019-02-22 | 2019-05-28 | 湖南莱博赛医用机器人有限公司 | A kind of device and method automatically extracting liquid sample |
| CN109813575B (en) * | 2019-02-22 | 2024-04-09 | 湖南莱博赛医用机器人有限公司 | Device and method for automatically extracting liquid sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107063767B (en) | 2019-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5893051B2 (en) | Alternating pneumatic membrane cell separation system | |
| CN102279118A (en) | Concentration gradient diffusion sampler for bioreactor on-line testing system | |
| CN105890923B (en) | A kind of aseptic micro online sampling device and sampling method | |
| CN104677675A (en) | Sampling device and biological reaction device with sampling device | |
| CN107641596A (en) | Multichannel automatic ration sterile sampling device and the bioreactor with the device | |
| CN103045713B (en) | Method for detecting trace amounts of microbes in a liquid sample containing multiple impurities | |
| CN203965191U (en) | Sampler and there is the biological reaction apparatus of this sampler | |
| CN107063767A (en) | A kind of sampler and fermentation tank sterile sampling method | |
| CN206350950U (en) | A kind of wastewater suspended substance Multi-stage module filter | |
| CN113375983A (en) | Sterile sampling device and sampling method thereof | |
| JPS61135580A (en) | Culture monitor apparatus | |
| CN1173161C (en) | A micro-sampling device for an on-line detection system of a biological reaction process | |
| CN116515611A (en) | Online sterile cell sampling system and sampling method | |
| CN106232829B (en) | Sample preparation device and method for preparing samples for sterility testing | |
| CN108441413A (en) | A kind of microcarrier suspension culture on-line period device and operating method | |
| CN206970605U (en) | A kind of gobbet automatic fitration bactericidal device | |
| CN215712991U (en) | Aseptic sampling device of microbial fermentation jar | |
| CN215050239U (en) | Bioreactor aseptic sampling kit | |
| CN206089654U (en) | Bacterium detecting device | |
| CN211302243U (en) | An adjustable filter aid | |
| CN204848870U (en) | Sample taking device | |
| JP2009236513A (en) | Sampler and sampling method using it | |
| CN208414394U (en) | A kind of enrichment facility of microorganism sewage water processing strain | |
| CN204661695U (en) | A kind of sampling unit detecting fermentative production sterile air | |
| CN214529033U (en) | A collection fungus culture apparatus for public health prevention branch of academic or vocational study |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190521 Termination date: 20210614 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |