CN107063767A - A kind of sampler and fermentation tank sterile sampling method - Google Patents
A kind of sampler and fermentation tank sterile sampling method Download PDFInfo
- Publication number
- CN107063767A CN107063767A CN201710445494.8A CN201710445494A CN107063767A CN 107063767 A CN107063767 A CN 107063767A CN 201710445494 A CN201710445494 A CN 201710445494A CN 107063767 A CN107063767 A CN 107063767A
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- Prior art keywords
- pipeline
- membrane tube
- separation membrane
- switch valve
- sampler
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
Abstract
The invention discloses a kind of sampler and fermentation tank sterile sampling method, wherein the device includes high temperature and high pressure gas generator, separate membrane tube, supporter and sample cell, the separation membrane tube is fixed on the supporter, the outer wall border of the separation membrane tube and extraneous directly contact, one end of the separation membrane tube is connected by the first pipeline with the high temperature and high pressure gas generator, voltage stabilization and current stabilization valve is provided with first pipeline, the other end of the separation membrane tube is connected by the second pipeline with the sample cell, first switch valve is provided with second pipeline.In the present invention by the way of high temperature and high pressure gas generator is set, high temperature and high pressure gas are allowd to eliminate the drop of residual in sampler pipeline, and preventing inside pipe wall from growing microorganism clogging pipeline, it is to avoid sample enters the problem of pollution occurs in separation membrane tube and blocks；Simultaneously after the completion of each sampling, gas back flush can be carried out to separation membrane tube, it is to avoid the problem of pipeline blockage.
Description
Technical field
Detection technique field is sampled the present invention relates to fermentation process, more particularly to a kind of sampler and fermentation tank are sterile
Sampling method.
Background technology
Bioreactor is the biological function having using enzyme or organism (such as microorganism), is carried out in vitro biochemical anti-
The apparatus system answered, it is a kind of biological function analog machine (container), such as fermentation tank, enzyme or cell reactor, food with
Fermentation (such as drinks, amino acid, organic acid), medical (antibiotic, interferon), environment (biodegradation), bioenergy (cellulose
Ethanol, oil-producing microalgae, biogas) etc. production and scientific research field extensive use.
The Effec-tive Function of bioreactor is maintained, that is, obtains the biological transformation ratio and minimum cost of maximum, it is necessary to right
Bioprocess in reactor is controlled, and its basis controlled is exactly that various parameters in reactor are detected.
The detection mode of bioreactor has two kinds of offline inspection and on-line checking.Offline inspection is by manual sampling, so
Afterwards, sample is sent to laboratory or instrument room, be measured by instrumentation.On-line checking be detecting instrument directly with instead
Answer device link together or detection sensor probe intercalation reaction device in detected.Advantage is that sampling and detection process can connect
It is continuous, rapid, accurate to complete, can in time, the dynamic change of accurate, continuous reflection reactor biochemical process, be conducive to biological anti-
Answer the optimization and control of process.
On-line detecting system generally comprises 2 parts：(1) analysis and detection technology and equipment (detection means)；(2) it will can examine
Survey the device i.e. sampler that device and inside reactor medium are combined.
Sampler of the prior art mainly includes two classes, wherein, a class is made up of pipeline and valve, and a class is point
From membrane filtration.The sampler of pipeline valve composition is disadvantageous in that：What is taken out is former state in reactor, contains thalline, original
Expect thick slag etc., often need that Instrument measuring could be entered after filtration treatment；To avoid the pollution of sampling process, it is necessary to which needs are matched somebody with somebody
Put bactericidal unit, such as steam, uviol lamp.For seperation film filtering and sampling, because the aperture of film prevents passing through for thalline, keep away
Exempted from external source pollution, weak point be the obstruction that impurity in liquid easily causes seperation film mesh, filtering stability difference, it is necessary to
Often cleaning or replacing.
The content of the invention
It is an object of the invention to provide the sampler that a kind of sampling efficiency is high, sampling quality is good, safe and convenient to use and
Fermentation tank sterile sampling method, is easily hindered with solving seperation film mesh in existing fermentation tank sterile sampling device and sampling method device
Plug, easily pollution cause the problem of sampling sample quality is poor, sampling efficiency is low.
To achieve the above object, the invention provides following scheme：The present invention provides a kind of sampler, including high temperature height
Pressure gas generator, separation membrane tube, supporter and sample cell, the separation membrane tube are fixed on the supporter, the separation
The outer wall border of membrane tube and extraneous directly contact, one end of the separation membrane tube passes through the first pipeline and the high temperature and high pressure gas
Generator is connected, and voltage stabilization and current stabilization valve is provided with first pipeline, and the other end of the separation membrane tube passes through the second pipeline
It is connected with the sample cell, first switch valve is provided with second pipeline.
It is preferred that, be provided with fluid passage on the supporter, the two ends of the separation membrane tube respectively with it is corresponding described
Fluid passage is connected, and the fluid passage is connected with first pipeline and second pipeline respectively.
It is preferred that, second switch valve is additionally provided with second pipeline, the second switch valve is close to one of institute
State fluid passage.
It is preferred that, the junction at the two ends and the fluid passage of the separation membrane tube is provided with sealing structure.
It is preferred that, the material of the separation membrane tube is porous ceramics or organic high molecular layer；It is preferred that, the porous ceramics
Or the aperture of organic high molecular layer is at 0.1-0.2 μm.
It is preferred that, second switch valve is additionally provided with second pipeline, the second switch valve is close to the seperation film
Pipe.
It is preferred that, the sampler also includes draw-out device, and the draw-out device passes through the 3rd pipeline and described second
Pipeline is connected, and the 3rd pipeline is located at the first switch valve with the junction of second pipeline and separates membrane tube with described
Between.
It is preferred that, it is provided with the 3rd switch valve on the 3rd pipeline.
Above-mentioned sampler is applied in the present invention, a kind of fermentation tank sterile sampling method is additionally provided, it is characterised in that bag
Include following steps：
By the supporter in sampler and separation membrane tube insertion fermentation tank；
Voltage stabilization and current stabilization valve, first switch valve, second switch valve and the 3rd switch valve in the sampler are opened, and are led to
Cross high temperature high pressure gas generator and be passed through high temperature and high pressure gas into the sampler and sterilized；
Close the high temperature and high pressure gas generator after the completion of sterilizing, and by the voltage stabilization and current stabilization valve and the first switch
Valve is closed, and the second switch valve and the 3rd switch valve are opened, and the draw-out device in the sampler starts, described to take out
Take device by the zymotic fluid outside the separation membrane tube, be pumped into the inside of the separation membrane tube；
The draw-out device closes the draw-out device after the completion of extracting, and the voltage stabilization and current stabilization valve, described first are opened
Close valve and the second switch valve is opened, the 3rd switch valve is closed, the high temperature and high pressure gas generator starts, described
The high temperature and high pressure gas that high temperature and high pressure gas generator is produced promote the zymotic fluid inside the separation membrane tube to enter the sampling
Sample cell in device.
It is preferred that, after the completion of sampling, keep the voltage stabilization and current stabilization valve to open, and by the first switch valve, described second
Switch valve and the 3rd switch valve are closed, and the high temperature and high pressure gas that the high temperature and high pressure gas generator is produced are to the separation
Membrane tube is backwashed.
The present invention achieves following beneficial effect relative to prior art：
1st, in the present invention by the way of high temperature and high pressure gas generator is set so that high temperature and high pressure gas can be eliminated and taken
The drop of residual in sampling device pipeline, it is to avoid separation membrane tube outgoing zymotic fluid produces dense after being merged after entering in pipe with residual liquid
The problem of spending error, and then the uniformity of sample concentration and the outer zymotic fluid concentration of pipe in pipe is ensure that, while high temperature and high pressure gas
It is also possible to prevent in pipeline to produce microorganism, it is to avoid fermentation broth sample enters the problem of polluting in separation membrane tube, also
Inside pipe wall is prevented to grow the problem of microorganism causes to block inside separation membrane tube.
2nd, the material of separation membrane tube is porous ceramics or organic high molecular layer in the present invention, is resistant to high-temperature sterilization, aperture exists
Between 0.1-0.2 μm, microorganism can not pass through, and can effectively prevent there is thalline, the sample (zymotic fluid) of taking-up in sampling sample
There is no impurity, be not required to filtration treatment and just directly determined suitable for a variety of analytical instrument.
3rd, in the present invention in each sampling process or after the completion of, high temperature and high pressure gas can be carried out to separation membrane tube
Back flush, washes the thalline for being attached to pipeline outer wall, keep the long-term cleaning of pipeline outer wall, it is to avoid the life of sample flow
Thing contamination and plugging, improves sampling efficiency.
4th, in the present invention before work is sampled, the HTHP gas of high temperature and high pressure gas generator generation is first passed through
Body carries out sterilization treatment to whole sampler, it is ensured that pollution sources are not present in sampler in itself, it is to avoid due to sampler
Pollutant cause the contaminated problem of whole fermentation tank, improve the security performance of sampling process.
5th, due to containing abundant nutrients in the sample that fermentation tank takes out using sampler, easily in circulation duct
(including separation membrane tube, the first pipeline, the second pipeline, fluid passage etc.) middle generation residual, cause the pollution of circulation duct, this hair
The high temperature and high pressure gas produced in bright by high temperature and high pressure gas generator sterilize to whole circulation duct, can avoid circulation
The line clogging that pipeline pollution is caused, improves the stability of pipeline flow.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is the overall structure diagram of inventive sampling device；
Wherein, 1- high temperature and high pressure gas generator, 2- separation membrane tube, 3- supporters, 4- sample cells, the pipelines of 5- first, 6-
Voltage stabilization and current stabilization valve, the pipelines of 7- second, 8- first switches valve, 9- fluid passages, 10- second switches valve, 11- sealing structures, 12- take out
Take device, the switch valves of 13- the 3rd, the pipelines of 14- the 3rd.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
It is an object of the invention to provide the sampler that a kind of sampling efficiency is high, sampling quality is good, easy to use and fermentation
Tank sterile sampling method, easily blocked with solving seperation film mesh in existing fermentation tank sterile sampling device and sampling method device,
Easily pollution causes the problem of sampling sample quality is poor, sampling efficiency is low.
In order to facilitate the understanding of the purposes, features and advantages of the present invention, it is below in conjunction with the accompanying drawings and specific real
Applying mode, the present invention is further detailed explanation.
As shown in figure 1, the present invention provides a kind of sampler, including high temperature and high pressure gas generator 1, separation membrane tube 2, branch
Support body 3 and sample cell 4, the separation membrane tube 2 are fixed on the supporter 3, the outer wall border and the external world of the separation membrane tube 2
Directly contact, one end of the separation membrane tube 2 is connected by the first pipeline 5 with the high temperature and high pressure gas generator 1, described
Voltage stabilization and current stabilization valve 6 is provided with first pipeline 5, the other end of the separation membrane tube 2 passes through the second pipeline 7 and the sample cell 4
It is connected, first switch valve 8 is provided with second pipeline 7.
Inventive sampling device in the course of the work, produces high temperature and high pressure gas, high temperature in high temperature and high pressure gas generator 1
Gases at high pressure are transported in the pipe-line system of sampler by the control stabilization of voltage stabilization and current stabilization valve 6, are carried out in sampler
High temperature and high pressure gas can be sterilized to pipe-line system before sampling work, and when being sampled work, high temperature and high pressure gas are pushed away
The dynamic sample that channel interior is infiltrated through by separation membrane tube 2 so that sample enters in sample cell 4, waits to be used；Finish in sampleman
After, the first switch valve 8 being arranged on the second pipeline 7 is closed so that high temperature and high pressure gas are in the hole of separation membrane tube 2
Outflow, forms gas back-flushing, washes the thalline for being attached to the separation pipeline outer wall of membrane tube 2, keeps the long-term clear of pipeline outer wall
It is clean.Wherein, supporter 3 is the cylindrical structure processed by organic material rod, and a diameter of 1.0-2.5cm separates membrane tube 2
Internal diameter footpath is 0.5-2.0mm.
Very strong active force (such as to tube wall impulsive force) is produced when being moved due to high temperature and high pressure gas in pipeline, it is easily right
Pipeline produces damage, or to line connection (junction of such as the first pipeline 5 with separating membrane tube 2, if the first pipeline 5 is in height
Sharp pounding or shake are produced in the presence of warm gases at high pressure, the structural strength of junction will be destroyed, service life is influenceed and close
Envelope property) sealing property produce influence, in order to ensure the service life and sealing of pipeline, in the supporter 3 in the present invention
On be provided with fluid passage 9, the two ends of the separation membrane tube 2 are connected with the corresponding fluid passage 9 respectively, the fluid
Passage 9 is connected with first pipeline 5 and second pipeline 7 respectively.Used in the present invention and fluid is set on supporter 3
The mode of passage 9 so that the two ends of separation membrane tube 2 first pipeline 5 and the phase of the second pipeline 7 are distinguished by corresponding fluid passage 9
Connection, it is to avoid separation membrane tube 2 is directly connected with the first pipeline 5 and the second pipeline 7, it is ensured that pipeline (including separation membrane tube 2,
First pipeline 5, second pipeline 7 etc.) service life and sealing, wherein, fluid passage 9 is U-shaped, i.e., U-shaped diffuser, pipe
Road internal diameter is 0.5-2.0mm.
In order to avoid the harmful effect that backwash process high temperature gases at high pressure are produced to the second pipeline 7, such as gas impacts
Cause the service life of the second pipeline 7 low, or because the second long pipeline 7 causes asking for backwash gas insufficient pressure
Second switch valve 7 is additionally provided with second pipeline 7 in topic, the present invention, the second switch valve 7 is close to one of institute
State fluid passage 9 (this fluid passage 9 is the fluid passage 9 being connected with the second pipeline 7)；Closed in backwash process is carried out
Second switch valve 7, shortens flow distance of the high temperature and high pressure gas in the second pipeline 7.
The junction at the two ends and the fluid passage 9 of heretofore described separation membrane tube 2 is provided with sealing structure 11, protects
Card separation membrane tube 2 and the sealing property of fluid passage 9, wherein, sealing structure 11 can be sealing ring.
The material of heretofore described separation membrane tube 2 is porous ceramics or organic high molecular layer；It is preferred that, the porous pottery
The aperture of porcelain or organic high molecular layer is at 0.1-0.2 μm.
In order to avoid the harmful effect that backwash process high temperature gases at high pressure are produced to the second pipeline 7, such as gas impacts
Cause the service life of the second pipeline 7 low, or because the second long pipeline 7 causes asking for backwash gas insufficient pressure
Second switch valve 7 is additionally provided with second pipeline 7 in topic, the present invention, the second switch valve 7 is close to the seperation film
Pipe 2 (i.e. close to junction of second pipeline 7 with separating membrane tube 2), closes second switch valve 7, contracting in backwash process is carried out
Flow distance of the short high temperature and high pressure gas in the second pipeline 7.
In order to improve the sampling efficiency of sampler, heretofore described sampler also includes draw-out device 12, described
Draw-out device 12 is connected by the 3rd pipeline 14 with second pipeline 7, the 3rd pipeline 14 and second pipeline 7
Junction is located at the first switch valve 8 and separated with described between membrane tube 2, and draw-out device 12 extracts pipeline system in sampling process
The internal gas of system (including separating membrane tube 2, the first pipeline 5, the second pipeline 7, the 3rd pipeline 14 etc.) so that inside pipe-line system
Negative pressure is formed, and then external fluid is quickly entered inside pipe-line system in the presence of negative pressure, sampling efficiency is improved.
Be provided with the 3rd switch valve 13 on heretofore described 3rd pipeline 14, realize insertion to the 3rd pipeline 14 or
Close.
Above-mentioned sampler is applied in the present invention, a kind of fermentation tank sterile sampling method is additionally provided, comprises the following steps：
Supporter 3 in sampler and separation membrane tube 2 are inserted into fermentation tank；
Voltage stabilization and current stabilization valve 6, first switch valve 8, the switch valve 13 of second switch valve 7 and the 3rd in the sampler are opened
Open, and high temperature and high pressure gas are passed through into the sampler by high temperature and high pressure gas generator 1 and sterilized；
The high temperature and high pressure gas generator 1 is closed after the completion of sterilizing, and the voltage stabilization and current stabilization valve 6 and described first are opened
Close valve 8 to close, the second switch valve 7 and the 3rd switch valve 13 are opened, and the draw-out device 12 in the sampler is opened
Dynamic, the zymotic fluid outside the separation membrane tube 2 is pumped into the inside of the separation membrane tube 2 by the draw-out device 12；
The draw-out device 12 closes the draw-out device 12 after the completion of extracting, and by the voltage stabilization and current stabilization valve 6, described the
One switch valve 8 and the second switch valve 7 are opened, and the 3rd switch valve 13 is closed, the high temperature and high pressure gas generator 1
Start, the high temperature and high pressure gas that the high temperature and high pressure gas generator 1 is produced promote the zymotic fluid inside the separation membrane tube 2 to enter
Enter the sample cell 4 in the sampler.
Further, after the completion of sampling, keep the voltage stabilization and current stabilization valve 6 to open, and by the first switch valve 8,
The second switch valve 7 and the 3rd switch valve 13 are closed, the HTHP gas that the high temperature and high pressure gas generator 1 is produced
Body is backwashed to the separation membrane tube 2.
Specific case is applied in the present invention to be set forth the principle and embodiment of the present invention, above example
Illustrate the method and its core concept for being only intended to help to understand the present invention；Simultaneously for those of ordinary skill in the art, according to
According to the thought of the present invention, it will change in specific embodiments and applications.In summary, this specification content
It should not be construed as limiting the invention.
Claims (10)
1. a kind of sampler, it is characterised in that including high temperature and high pressure gas generator, separation membrane tube, supporter and sample cell,
The separation membrane tube is fixed on the supporter, the outer wall border of the separation membrane tube and extraneous directly contact, the separation
One end of membrane tube is connected by the first pipeline with the high temperature and high pressure gas generator, and voltage stabilizing is provided with first pipeline
Flow stabilizing valve, the other end of the separation membrane tube is connected by the second pipeline with the sample cell, is set on second pipeline
There is first switch valve.
2. sampler according to claim 1, it is characterised in that fluid passage is provided with the supporter, described
Separation membrane tube two ends be connected respectively with the corresponding fluid passage, the fluid passage respectively with first pipeline and
Second pipeline is connected.
3. sampler according to claim 2, it is characterised in that be additionally provided with second switch on second pipeline
Valve, the second switch valve is close to fluid passage one of them described.
4. sampler according to claim 2, it is characterised in that the two ends of the separation membrane tube and the fluid passage
Junction be provided with sealing structure.
5. sampler according to claim 1, it is characterised in that the material of the separation membrane tube is porous ceramics or had
Organic polymeric membrane；It is preferred that, the aperture of the porous ceramics or organic high molecular layer is at 0.1-0.2 μm.
6. sampler according to claim 1, it is characterised in that be additionally provided with second switch on second pipeline
Valve, the second switch valve is close to the separation membrane tube.
7. the sampler according to claim any one of 1-6, it is characterised in that the sampler also includes extracting dress
Put, the draw-out device is connected by the 3rd pipeline with second pipeline, the 3rd pipeline and second pipeline
Junction is located at the first switch valve and separated with described between membrane tube.
8. sampler according to claim 7, it is characterised in that be provided with the 3rd switch valve on the 3rd pipeline.
9. a kind of fermentation tank sterile sampling method, it is characterised in that comprise the following steps：
By the supporter in sampler and separation membrane tube insertion fermentation tank；
Voltage stabilization and current stabilization valve, first switch valve, second switch valve and the 3rd switch valve in the sampler are opened, and pass through height
Warm high pressure gas generator is passed through high temperature and high pressure gas into the sampler and sterilized；
The high temperature and high pressure gas generator is closed after the completion of sterilizing, and the voltage stabilization and current stabilization valve and the first switch valve are closed
Close, the second switch valve and the 3rd switch valve are opened, and the draw-out device in the sampler starts, described to extract dress
Put the zymotic fluid outside the separation membrane tube, be pumped into the inside of the separation membrane tube；
The draw-out device closes the draw-out device after the completion of extracting, and by the voltage stabilization and current stabilization valve, the first switch valve
Opened with the second switch valve, the 3rd switch valve is closed, the high temperature and high pressure gas generator starts, the high temperature
The high temperature and high pressure gas that high pressure gas generator is produced promote the zymotic fluid inside the separation membrane tube to enter the sampler
In sample cell.
10. fermentation tank sterile sampling method according to claim 9, it is characterised in that after the completion of sampling, keeps described steady
Press flow stabilizing valve to open, and the first switch valve, the second switch valve and the 3rd switch valve are closed, the high temperature is high
The high temperature and high pressure gas that pressure gas generator is produced are backwashed to the separation membrane tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710445494.8A CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710445494.8A CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107063767A true CN107063767A (en) | 2017-08-18 |
| CN107063767B CN107063767B (en) | 2019-05-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710445494.8A Expired - Fee Related CN107063767B (en) | 2017-06-14 | 2017-06-14 | A kind of sampler and fermentor sterile sampling method |
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| CN107621550A (en) * | 2017-11-08 | 2018-01-23 | 苏州市苏测检测技术有限公司 | Food elution detection integration apparatus |
| CN109813575A (en) * | 2019-02-22 | 2019-05-28 | 湖南莱博赛医用机器人有限公司 | A kind of device and method automatically extracting liquid sample |
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|---|---|---|---|---|
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| CN109813575A (en) * | 2019-02-22 | 2019-05-28 | 湖南莱博赛医用机器人有限公司 | A kind of device and method automatically extracting liquid sample |
| CN109813575B (en) * | 2019-02-22 | 2024-04-09 | 湖南莱博赛医用机器人有限公司 | Device and method for automatically extracting liquid sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107063767B (en) | 2019-05-21 |
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