CN104677675A - Sampling device and biological reaction device with sampling device - Google Patents

Sampling device and biological reaction device with sampling device Download PDF

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Publication number
CN104677675A
CN104677675A CN201310643371.7A CN201310643371A CN104677675A CN 104677675 A CN104677675 A CN 104677675A CN 201310643371 A CN201310643371 A CN 201310643371A CN 104677675 A CN104677675 A CN 104677675A
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China
Prior art keywords
sampling
peristaltic pump
pipeline
reactor
sampler
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Pending
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CN201310643371.7A
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Chinese (zh)
Inventor
唐寅
张明
黄明志
杭海峰
易小平
张建坤
郭谋之
何翌清
曾良平
金巍巍
徐奇聪
张伟平
马文峰
张大鹤
滕小诺
庄英萍
张嗣良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUOQIANG BIOCHEMICAL ENGINEERING EQUIPMENT Co Ltd SHANGHAI
East China University of Science and Technology
Shanghai Guoqiang Bioengineering Equipment Co Ltd
Original Assignee
GUOQIANG BIOCHEMICAL ENGINEERING EQUIPMENT Co Ltd SHANGHAI
East China University of Science and Technology
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Application filed by GUOQIANG BIOCHEMICAL ENGINEERING EQUIPMENT Co Ltd SHANGHAI, East China University of Science and Technology filed Critical GUOQIANG BIOCHEMICAL ENGINEERING EQUIPMENT Co Ltd SHANGHAI
Priority to CN201310643371.7A priority Critical patent/CN104677675A/en
Publication of CN104677675A publication Critical patent/CN104677675A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a sampling device and a biological reaction device with the sampling device, wherein the sampling device is used for sampling reactants in a bioreactor and comprises a heating cooler, a gas pipeline, a first pinch valve, a second pinch valve, a sampling pipeline, a first peristaltic pump and a second peristaltic pump, wherein the gas pipeline passes through the heating cooler; one end of the gas pipeline is connected to an air filter; the other end of the gas pipeline is an exhaust hole; the first pinch valve is arranged on the gas pipeline and positioned between the air filter and the heating cooler; the second pinch valve is arranged on the gas pipeline and positioned between the heating cooler and the exhaust hole; the sampling pipe passes through the heating cooler; one end of the sampling pipeline is positioned in the bioreactor; the other end of the sampling pipeline is a sample outlet; the first peristaltic pump is arranged on the sampling pipeline and positioned between the bioreactor and the heating cooler; and the second peristaltic pump is arranged on the sampling pipeline and positioned between the heating cooler and the sampler outlet, wherein the parts, passing through the heating cooler, of the gas pipeline and the sampling pipeline are communicated.

Description

Sampler and there is the biological reaction apparatus of this sampler
Technical field
The present invention relates to technological field of biochemistry, and in particular to a kind of sampler and the biological reaction apparatus with this sampler.
Background technology
Bio-reactor be utilize enzyme or biosome (as microorganism) the biological function that has, carry out the apparatus system of biochemical reaction in vitro, it is a kind of biological function analog machine, as fermentation tank, enzyme or cell reactor etc.Important application is had in drinks, medicine production, concentrated jam, fruit juice fermentation, organic pollutant degradation.
Often need to carry out sampling operation in the biological culture process of bio-reactor.The requirement of the aspects such as the control that this operation will meet aseptic, sampling amount usually and the difference control quantitatively, repeatedly sampled.
In the prior art,
As Chinese patent, number of patent application: CN200520073106.0, it discloses a kind of New type fermentation automatic sampling valve, be made up of liquid inlet, steam inlet, gear train, sample tap and by-pass valve control, it is characterized in that: bottom automatic sampling valve, be provided with the servomotor with controller, steam inlet and sample tap are located at sample valve both sides, one end is near liquid inlet, one end is positioned at valve body lower portion, and the interface disc face being positioned at the liquid inlet place at sample valve top is provided with sealing ring washer.
Also as: as Chinese patent, number of patent application: CN201020102452.8, it discloses a kind of Special sampling valve for fermentation tank, comprise fermentation tank, the first valve and the second valve, the import of described first valve is connected with fermentation tank, the outlet of the first valve is connected with the import of the second valve, the valve base being positioned at its exit at this first valve offers sample tap, at this sample tap place, sample valve is installed, the utility model advantage is: structure is simple, by installing a valve again on former valvular basis, form the structure of valve on valve.
The common trait of above two sections of patents is all the sampler be made up of pipeline and valve member, and energy sample (former state) that is automatic, directly taking-up inside reactor, has steam sterilization and packing washer, prevent sampling from polluting.
As Chinese patent, number of patent application: CN200910034515.2 " system of detecting concentration of sugar in fermentation process on line ", the feature of this patent directly from fermentation tank, samples (former state) by constant flow pump, then, after sample mixes in proportion with pure water (dilution), be sent on detecting instrument and measure.Which employs constant flow pump, is not open or close sampling by valve.By the uniflux of liquid, avoid the pollution to reactor (fermentation tank).
As Chinese patent, number of patent application: CN02138376.6 " a kind of sampling air mix facilities of bioprocesses on-line detecting system " this patent places a ceramic membrane filter part at sampling probe end (being connected with fermentation tank), liquid in reactor continuous print in membrane filters, the filtered fluid of trace is drawn (not being former state) by pipeline, after an air mix facilities, enter detector and measure.Ceramic membrane aperture between 0.1 ~ 0.2pm, microorganism not by, can effectively prevent reactor (fermentation tank) from polluting.
As United States Patent (USP), patent No. US4,942,770, Se1fert " a kind of sterile sampling device ".Be connected with reactor by a T-valve, sampling is completed by conversion valve passage, namely first connect with fermentation tank, (forward) flows into sampling pipe, then convert valve (to close and the UNICOM sending out tank inside liquor-saturated, open the UNICOM with external pipe), the sample (former state) in sampling pipe is oppositely sent into next step air mix facilities or detecting instrument by liquor pump.Due to the switching of T-valve, avoid inside reactor and contact with the direct of the external world, thus play the effect of pollution-free sampling.
As United States Patent (USP), patent No. US5,409,841, Chow " sampler of ultraviolet disinfection and method ", adopt uviol lamp to carry out sterilizing to sampling pipe and container, do not adopt steam sterilizing.
As United States Patent (USP), patent No. US4,695,551, Samhaber, " bio-reactor sampler ", adopts semipermeable membrane filtering and sampling.Semi-permeable diaphragm can be isolated microorganism and be passed through, and avoids polluting, and increases a semi-permeable diaphragm shaking device simultaneously, prevents the obstruction of fenestra.
As United States Patent (USP), patent No. US5,914,092, Moon, " fermentation tank sample cock steam sterilizer ", relates to a kind of about improving the apparatus and method of steam to sample cock and pipeline sterilizing efficiency.
As United States Patent (USP), patent No. US6,860,162, Jaeger, " liquid sampling and method ", when wine fermentation tank samples, devise a settler, sampling probe can avoid sediment in fermentation liquor, prevents from blocking.
In sum, the sampling operation in biological respinse of the prior art mainly contains two types, and a class is that diffusion barrier filters, and a class is made up of pipeline and valve.
But above sampling operation method exists following defect and deficiency, specific as follows:
Diffusion barrier filtering and sampling: the aperture due to film prevents passing through of thalline, avoids external source and pollutes.But for needing the detection of component in solid content, some born of the same parents in detection nutrient solution, carrying out the requirement of experiment such as microscopy to biology, this mode can not be suitable for.
The sampler that pipeline and valve form: the needs that above-mentioned experiment detects can be met.Also there is following limitation simultaneously:
One, sample to preventing the control mode polluted:
1, environmental Kuznets Curves adds sanitizer (alcohol) docking port and carries out disinfection: require high to environment for use, and sanitizer has interference (component, microscopy form etc.) to taking-up sample;
2, steam carries out disinfection to pipeline and interface: need during use to configure steam, requires complicated (withstand voltage, anti-scald, discharge of steam), be applicable to larger metal reaction device to pipeline, experimental provision;
3, ultraviolet sterilization: carry out complicacy to operating band, and the sampling of active microorganism is brought to the impact that cannot assess.
4, three-way switch valve mode: biological culture generally adopts diaphragm valve, there is dead angle in T-valve structure, cannot assess the chance polluted.
Two, to the control of sampling process discharging of waste liquid amount:
In sampling process, need first waste liquid remaining in pipeline to be got rid of, taking-up sample liquid just can be enable consistent with the nutrient solution in culture apparatus.It is individual process slowly that microorganism is cultivated, and what have takes more than one day, and what have takes many skies.If it is more to sample number of times in whole process, and when nutrient solution cumulative volume is limited, just particularly important to the control of the remaining discharging of waste liquid of pipeline during every sub-sampling amount.
As from the foregoing, the sampler in biological culture process of the prior art, cannot meet the requirement of the aspect such as control and the difference control quantitatively, repeatedly to sample of rigorous aseptic, sampling amount.
Summary of the invention
In view of this, the present invention is intended to solve the sampler in biological culture process of the prior art, cannot meet the technical matters such as requirement of the aspect such as control and the difference control quantitatively, repeatedly to sample of rigorous aseptic, sampling amount.
For solving the problem, the invention provides a kind of sampler, for sampling the reactant in bio-reactor, comprising: heating refrigeratory; Gas pipeline, through described heating refrigeratory, one end is connected to air strainer, and the other end is exhausr port; First pinch valve, is arranged on described gas pipeline, and between described air strainer and heating refrigeratory; Second pinch valve, is arranged on described gas pipeline, and between described heating refrigeratory and exhausr port; Sampling pipe, through described heating refrigeratory, one end is arranged in bio-reactor, and the other end is sample export; First peristaltic pump, is arranged on described sampling pipe, and between bio-reactor and heating refrigeratory; Second peristaltic pump, is arranged on described sampling pipe, and between heating refrigeratory and sample export; Wherein, described gas pipeline communicates with the part of described sampling pipe through described heating refrigeratory.
Further, described gas pipeline and described sampling pipe pass the part of described heating refrigeratory is same pipeline.
Further, described sampler, also comprises: the second heating refrigeratory, is arranged between described second pinch valve and exhausr port; 3rd heating refrigeratory, is arranged between described second peristaltic pump and sample export.
Further, described pipeline is flexible silicone tube.
Further, described air strainer is used for filtering air, passes into filtrated air to described pipeline.
Further, described sampler also comprises controller, and described controller comprises: input-output unit; Central controller, is connected with described input-output unit; Multicircuit relay, is connected to described first pinch valve, the second pinch valve, the first peristaltic pump and the second peristaltic pump; Power module, is connected with described multicircuit relay.
Further, described central controller is programmable logic controller (PLC).
Further, described input-output unit is touch-screen.
The present invention also provides a kind of biological reaction apparatus, and it comprises: bio-reactor or analytical equipment, and the described above arbitrary sampler be connected with described bio-reactor or analytical equipment.
In sum, sampler provided by the invention and there is the biological reaction apparatus of this sampler, owing to adopting filtrated air pressurize, and under ensureing the prerequisite of germ-free condition by performer each on above-mentioned sampling line, living body biological can be taken out carry out analyzing and microscopy, avoid the situation of high temperature interference suffered by biomorph simultaneously.
Secondly, pressurize is carried out owing to adopting filtrated air in pipeline, namely by the reversion of peristaltic pump, with filtrated air, the residual liquid blowback of inserting in sampled liquid in sebific duct is returned in sampled liquid, both avoided the interference of residual liquid to real time sample analysis result, and also made usually required remaining waste discharge rate reduce to zero; The time simultaneously relying on peristaltic pump to rotate forward and the presetting of flow, make the quantification that each sampling amount can realize based on peristaltic pump precision.
Finally, due to the sampling operation step of the program setting by controller, sampling operation can be made to coordinate external automatic analyzer to carry out automatic sampling and on-line analysis, manual sampling mode also can be utilized temporarily to sample off-line analysis.
Accompanying drawing explanation
Figure 1 shows that the structural representation of the sampler that one embodiment of the invention provides; Figure 2 shows that the structural representation of the sampler that one embodiment of the invention provides;
Figure 3 shows that the structural representation of the sampler that another embodiment of the present invention provides;
Figure 4 shows that the structural representation of the sampler that another embodiment of the present invention provides;
Figure 5 shows that the structural representation of the sampler that another embodiment of the present invention provides;
Figure 6 shows that the structural representation of the sampler that another embodiment of the present invention provides.
Embodiment
For making object of the present invention, feature becomes apparent, and is further described the specific embodiment of the present invention below in conjunction with accompanying drawing.
Embodiment one
Refer to Fig. 1, it is depicted as the structural representation of the sampler provided for one embodiment of the invention.
This sampler, for sampling the reactant in bio-reactor, comprising: heating refrigeratory 130; Gas pipeline 121, through described heating refrigeratory 130, one end is connected to air strainer 110, and the other end is exhausr port; First pinch valve 141, is arranged on described gas pipeline, and between described air strainer 110 and heating refrigeratory 130; Second pinch valve 142, is arranged on described gas pipeline, and between described heating refrigeratory 130 and exhausr port; Sampling pipe 122, through described heating refrigeratory 130, one end is arranged in bio-reactor 100, and the other end is sample export; First peristaltic pump 151, is arranged on described sampling pipe 122, and between bio-reactor 100 and heating refrigeratory 130; Second peristaltic pump 152, is arranged on described sampling pipe 122, and between heating refrigeratory and sample export; Wherein, described gas pipeline 121 communicates with the part of described sampling pipe 122 through described heating refrigeratory 130.
In embodiments of the present invention, the part that described gas pipeline 121 and described sampling pipe 122 pass described heating refrigeratory 130 is same pipeline.
In embodiments of the present invention, described gas pipeline 121 is flexible silicone tube with described sampling pipe 122.
The concrete steps that the sampler utilizing the embodiment of the present invention to provide carries out sampling are as follows:
One, sample to preventing the control polluted:
1, all devices of this device all adopt clamp mode, such as: pinch valve, peristaltic pump, heating and refrigeratory, make pinch valve, peristaltic pump, heating directly contacts with sampled nothing with devices such as refrigeratorys.
2, described gas pipeline 121 adopts flexible silicone tube with described sampling pipe 122, described gas pipeline 121 is furnished with air strainer 110, described sampling pipe 122 and culture apparatus, as bio-reactor 100, realize connection in place, described gas pipeline 121 presss from both sides dead or sterile dressing with described sampling pipe 122 with extraneous interface.High-pressure sterilizing pot sterilizing is put into together with culture apparatus before using.Only need operate under the required environmental baseline of incubation itself during use, pipeline be snapped in pinch valve, peristaltic pump, heating refrigeratory.Because sampling pipe 122 presss from both sides extremely by the second pinch valve 142, second peristaltic pump 152, now decontrol described gas pipeline 121 when being connected with extraneous interface with described sampling pipe 122, the pollution of connection procedure to nutrient solution can not be produced.
3, the sampling pipe 122 after the second pinch valve 142, second peristaltic pump 152 and the connection of extraneous non-sterile pipeline, miscellaneous bacteria may be caused in long-time incubation to cross the second pinch valve 142, second peristaltic pump 152 to be caused the pollution in culture apparatus, adopts filtrated air (pressurized air filters through above-mentioned air strainer) to carry out aseptic pressurize to system, sample complete second pinch valve 142 of opening and blown off by the drop adhered on duct wall; Simultaneously according to bus moral disinfection principle, heating refrigeratory 130 pairs of sampling pipes 122 are utilized to carry out heat insulation, inner along the reverse breeding of pipeline, pollution culture apparatus to stop miscellaneous bacteria.May have an impact to component, microscopy profile etc. through this section of high-temperature pipe as taken out sample, can when sampling by switching to refrigerating mode to lower the temperature to pipeline heating mode.
Two, to the control of sampling process discharging of waste liquid amount:
1, owing to taking above-mentioned aseptic controlling measure, ensure that the germ-free condition of pipe interior, at sampling front opening first pinch valve 141, reversed by the first peristaltic pump 151, as being rotated counterclockwise, the remaining liquid in sampling pipe in culture apparatus 122 is returned in sampler.Make the first peristaltic pump 151, second peristaltic pump 152 rotate forward again, as turned clockwise, the sample now taken out is be up-to-date style, and without effluent discharge.
2, according to sampling amount demand, by determining the demarcation of lower corresponding peristaltic pump flow to states such as peristaltic pump rotating speed, internal diameter of the pipeline, peristaltic pump two ends pressure reduction, namely by the setting of the duration of runs of peristaltic pump, sampling amount is controlled.When reaching required sampling amount, the first peristaltic pump 151 is reversed, as being rotated counterclockwise, the second peristaltic pump 152 continues to rotate forward, as turned clockwise.Now with filtrated air pipeline access point for separation, the residual liquid of nearly first peristaltic pump 151 end is pressed completely into culture apparatus, the taking-up sample of nearly second peristaltic pump 152 end flows into external sampler or analyser completely under the pushing tow of filtrated air, and whole process can not produce the excessive and waste liquid of sampling.
In embodiments of the present invention, refer to Fig. 2, this sampler also comprises controller 200, and described controller 200 comprises: input-output unit 210; Central controller 220, is connected with described input-output unit 210; Multicircuit relay 230, is connected to described first pinch valve, the second pinch valve, the first peristaltic pump and the second peristaltic pump; Power module 240, is connected with described multicircuit relay 230.
In embodiments of the present invention, described central controller can be programmable logic controller (PLC) or single-chip microcomputer.
In embodiments of the present invention, described input-output unit is touch-screen.
Based on above-mentioned pipeline, performer simple for structure, controller is equipped with Programmed control, can accomplish sampling time point, the sampling process time, sampling quantity repeatability control.Especially to the situation of long period, multiple repairing weld, avoid the error that manual operation brings, the situation limited to nutrient solution total amount also can meet request for utilization, thus meets the control overflow to sampling operation repeatability.
Embodiment two
Refer to Fig. 3, it is depicted as the structural representation of the sampler that another embodiment of the present invention provides.
In the present embodiment, described sampler, comprising: the first heating refrigeratory 331; Gas pipeline 321, through described first heating refrigeratory 331, one end is connected to air strainer 310, and the other end is exhausr port 323; First pinch valve 341, is arranged on described gas pipeline, and between described air strainer 310 and the first heating refrigeratory 331; Second pinch valve 342, is arranged on described gas pipeline, and between described first heating refrigeratory 331 and exhausr port 323; Sampling pipe 322, through described first heating refrigeratory 331, one end is arranged in bio-reactor 300, and the other end is sample export 324; First peristaltic pump 351, is arranged on described sampling pipe 322, and between bio-reactor 300 and the first heating refrigeratory 331; Second peristaltic pump 352, is arranged on described sampling pipe 322, and between the first heating refrigeratory 331 and sample export 324; Wherein, described gas pipeline 121 communicates with the part of described sampling pipe 122 through described heating refrigeratory 130.Describedly also to comprise: the second heating refrigeratory 332, is arranged between described second pinch valve and exhausr port; 3rd heating refrigeratory 333, is arranged between described second peristaltic pump 352 and sample export.
In the present embodiment, wherein heat refrigeratory 331,332,333 to adjust according to the distance with analytical equipment, one first can be adopted to heat refrigeratory 331, also second and third heating refrigeratory 332,333 can be adopted two each temp auto-controlleds of pipeline, or be combined into a two-channel modules by 332,333.The present invention is not limited thereto.
Embodiment three
Refer to Fig. 4, it is depicted as the structural representation of the sampler that another embodiment of the present invention provides.
Compared with embodiment one, the peristaltic pump 151 in embodiment one substitutes with pinch valve 442 in embodiment three, correspondingly changes provided by syringe pump 451 power of flapping of taking out of liquid in reactor.
The sampling performer of embodiment three, by syringe pump 451, peristaltic pump 452, pinch valve 441,442,443, liquid storage pipe 470, heating refrigeratory 431 or 432,433, flexible silicone tube and conduit coupling composition, then be combined into whole sampler with control module.
According to required sampling precision, syringe pump 451 peristaltic pump that also applicable accuracy is lower substitutes (because the two function is different, concrete program control step is shown in flow process 3 and describes).
Before using, emptying sebific duct 422, sample sebific duct 423 are wrapped up.High-pressure sterilizing pot sterilizing is put into together after liquid storage pipe 470, bio-reactor 400, filtrator 410 and sebific duct afterwards being connected by figure.By diagram, sebific duct is loaded each actuator after sterilizing completes, and the import of air filter is connected with air hose 421.
The idiographic flow that the sampler adopting embodiment three to provide carries out operating is as follows:
1, stopple coupon row residual solution in reactor:
Open pinch valve 441, peristaltic pump is pumped to suitable volumes; Close pinch valve 441, open pinch valve 442, syringe pump 451 extrudes gas, is pushed back in bio-reactor by liquid remaining in reactor stopple coupon.Open the refrigerating function of heating refrigeratory to reduce the temperature of wherein pipeline.
2, drawing liquid:
Syringe pump 451 is bled, and liquid needed amount in reactor is quantitatively drawn to liquid storage pipe 470.
3, sample is gone out:
Closed clip pipe valve 442; Open pinch valve 141, peristaltic pump 452 is rotated counterclockwise simultaneously, liquid is pressed into analytical equipment or sampling container.
4, blow:
Close peristaltic pump 452; Open pinch valve 443 and close rapidly, blowing out the micro liquid sticked on tube wall, to reduce the interference to lower sub-sampling.Open the heating function of heating refrigeratory.
5, stopple coupon residual liquid is pushed back:
Repeat step 1.
Step 1 and step 5 repeat, and function is consistent.Just need step 1 at the first sampling, each operation is afterwards the circulation of step 2-5.
Embodiment four
Refer to Fig. 5, it is depicted as the structural representation of the sampler that another embodiment of the present invention provides.
Compared with embodiment three, the key distinction is: the peristaltic pump 551 of syringe pump 451 in embodiment four is replaced.
Motion time following steps perform:
1, stopple coupon row residual solution in reactor:
Open pinch valve 542, peristaltic pump 551 is rotated counterclockwise simultaneously, pushes back participating in liquid in reactor stopple coupon in reactor with filtrated air.Open the refrigerating function of heating refrigeratory to reduce the temperature of wherein pipeline.
2, drawing liquid:
Peristaltic pump 551 turns clockwise, and liquid needed amount in reactor is quantitatively drawn to liquid storage pipe 170.
3, sample is gone out:
Closed clip pipe valve 542; Open pinch valve 541, peristaltic pump 552 is rotated counterclockwise simultaneously, liquid is pressed into analytical equipment or sampling container.
4, blow:
Close peristaltic pump 552; Open pinch valve 543 and close rapidly, blowing out the micro liquid sticked on tube wall, to reduce the interference to lower sub-sampling.Open the heating function of heating refrigeratory.
5, stopple coupon residual liquid is pushed back:
Repeat step 1.
Step 1 and step 5 repeat, and function is consistent.Just need step 1 at the first sampling, each operation is afterwards the circulation of step 2-5.
To above-described embodiment two to four, also can carry out change combination, include but not limited to:
According to the volume of sampling amount, determine the volume of liquid storage pipe, or directly substitute liquid storage pipe volume by duct length.
According to the distance of sampler and analytical equipment, heating refrigeratory can be independent, also can arrange separately or combine twin-channel heating refrigerating module at gas exhaust duct and produce kinds of pipeline.
According to specifically using operational requirements, pinch valve can be replaced with peristaltic pump, and peristaltic pump also can be replaced with pinch valve.
Change pinch valve, pinch valve into peristaltic pump, program control step is constant, and the rotation of the switch control rule and peristaltic pump that only relate to pinch valve controls (comprising sense of rotation to control).
Embodiment five
Refer to Fig. 6, it is depicted as the structural representation of the sampler that another embodiment of the present invention provides.
In the present embodiment, this sampler except with the combination of reactors in embodiment after sterilizing in high-pressure sterilizing pot in the lump application mode except, also can adopt the mode adopting diaphragm valve to be connected with the connecting pipe of reactor in this device, see Fig. 6.
Diaphragm valve 682,683 is welded to connect.Use front diaphragm valve 682 to close, be connected with sebific duct, air filter 610, liquid storage pipe 670, by gas outlet 623, go out after sample pipe 624 wraps up, put into high-pressure sterilizing pot sterilizing in the lump.
During use, the interface of diaphragm valve 682 with reactor sample valve 684 is connected, adopts the connected mode of the conventional secondary steam sterilizing of bio-reactor, realize bio-reactor and be connected (repeating no more) with the aseptic of sampler herein.
After aseptic connection, diaphragm valve 682 is opened, diaphragm valve 681,683 close, the program control step of automatic sampling is afterwards consistent with foregoing schemes and step.Diaphragm valve 681-683, sample valve 684 can be hand valves, also can be internally piloted valves, and its automatic program also can be incorporated in sampling controller.
In addition, the embodiment of the present invention additionally provides a kind of biological reaction apparatus, and it comprises: bio-reactor or analytical equipment, and the arbitrary sampler provided as above-described embodiment be connected with described bio-reactor or analytical equipment.
Such as: a whole set of biological reaction apparatus can combine with bio-reactor, also can combinationally use with analytical equipment, or three combinationally uses in the lump.Its concrete structure principle as above, does not repeat them here.
In sum, the sampler that the embodiment of the present invention provides and there is the biological reaction apparatus of this sampler, first owing to adopting filtrated air pressurize, and under ensureing the prerequisite of germ-free condition by performer each on above-mentioned sampling line, living body biological can be taken out carry out analyzing and microscopy, avoid the situation of high temperature interference suffered by biomorph simultaneously.
Secondly, pressurize is carried out owing to adopting filtrated air in pipeline, namely by the reversion of peristaltic pump, with filtrated air, the residual liquid blowback of inserting in sampled liquid in sebific duct is returned in sampled liquid, both avoided the interference of residual liquid to real time sample analysis result, and also made usually required remaining waste discharge rate reduce to zero; The time simultaneously relying on peristaltic pump to rotate forward and the presetting of flow, make the quantification that each sampling amount can realize based on peristaltic pump precision.
Finally, due to the sampling operation step of the program setting by controller, sampling operation can be made to coordinate external automatic analyzer to carry out automatic sampling and on-line analysis, manual sampling mode also can be utilized temporarily to sample off-line analysis.
Sampler provided by the invention, the requirement of the aspects such as the control meeting aseptic, sampling amount and the difference control quantitatively, repeatedly sampled.
Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention; have in any art and usually know the knowledgeable; without departing from the spirit and scope of the present invention; when being used for a variety of modifications and variations, therefore protection scope of the present invention is when being as the criterion depending on those as defined in claim.

Claims (9)

1. a sampler, for sampling the reactant in bio-reactor, is characterized in that, comprising:
Heating refrigeratory;
Gas pipeline, through described heating refrigeratory, one end is connected to air strainer, and the other end is exhausr port;
First pinch valve, is arranged on described gas pipeline, and between described air strainer and the first heating refrigeratory;
Second pinch valve, is arranged on described gas pipeline, and between described first heating refrigeratory and exhausr port;
Sampling pipe, through described first heating refrigeratory, one end is arranged in bio-reactor, and the other end is sample export;
First peristaltic pump, is arranged on described sampling pipe, and between bio-reactor and the first heating refrigeratory;
Second peristaltic pump, is arranged on described sampling pipe, and between the first heating refrigeratory and sample export;
Wherein, described gas pipeline communicates through the described first part heating refrigeratory with described sampling pipe.
2. sampler according to claim 1, is characterized in that, the part that described gas pipeline and described sampling pipe pass described heating refrigeratory is same pipeline.
3. sampler according to claim 1, is characterized in that, also comprises: the second heating refrigeratory, is arranged between described second pinch valve and exhausr port;
3rd heating refrigeratory, is arranged between described second peristaltic pump and sample export.
4. sampler according to claim 1, is characterized in that, described pipeline is flexible silicone tube.
5. sampler according to claim 1, is characterized in that, described air strainer is used for filtering air, passes into filtrated air to described pipeline.
6. sampler according to claim 1, is characterized in that, also comprises: controller, and described controller comprises:
Input-output unit;
Central controller, is connected with described input-output unit;
Multicircuit relay, is connected to described first pinch valve, the second pinch valve, the first peristaltic pump and the second peristaltic pump;
Power module, is connected with described multicircuit relay.
7. sampler according to claim 6, is characterized in that, described central controller is programmable logic controller (PLC).
8. sampler according to claim 6, is characterized in that, described input-output unit is touch-screen.
9. a biological reaction apparatus, is characterized in that, comprising: bio-reactor or analytical equipment, and be connected with described bio-reactor or analytical equipment as sampler arbitrary in claim 1 to 8.
CN201310643371.7A 2013-12-03 2013-12-03 Sampling device and biological reaction device with sampling device Pending CN104677675A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106153387A (en) * 2016-09-23 2016-11-23 天津中医药大学 A kind of automatic liquid-sampling device
CN107063767A (en) * 2017-06-14 2017-08-18 山东省科学院生物研究所 A kind of sampler and fermentation tank sterile sampling method
CN108519472A (en) * 2018-08-03 2018-09-11 中国科学院烟台海岸带研究所 A kind of more water layer aquatic environment multi-parameter original position on-line monitoring systems of aquafarm

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CN106153387A (en) * 2016-09-23 2016-11-23 天津中医药大学 A kind of automatic liquid-sampling device
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CN108519472A (en) * 2018-08-03 2018-09-11 中国科学院烟台海岸带研究所 A kind of more water layer aquatic environment multi-parameter original position on-line monitoring systems of aquafarm

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Application publication date: 20150603