CN104677675A - Sampling device and biological reaction device with sampling device - Google Patents

Abstract

The invention discloses a sampling device and a bioreactor with the sampling device, wherein the sampling device is used to sample the reactants in the bioreactor, which includes: a heating cooler; a gas pipeline passing through the heating Cooler, one end is connected to the air filter, the other end is an exhaust port; the first pinch valve is arranged on the gas pipeline, and is located between the air filter and the heating cooler; the second pinch valve , arranged on the gas pipeline, and between the heating cooler and the exhaust port; the sampling pipeline passes through the heating cooler, one end is located in the bioreactor, and the other end is a sample outlet; the first peristaltic The pump is arranged on the sampling pipeline, and is located between the bioreactor and the heating cooler; the second peristaltic pump is arranged on the sampling pipeline, and is located between the heating cooler and the sample outlet; wherein, the The gas pipeline communicates with the part of the sampling pipeline passing through the heating cooler.

Description

Sampling device and biological reaction device with the sampling device

technical field

The invention relates to the technical field of biochemistry, and in particular to a sampling device and a biological reaction device with the sampling device.

Background technique

A bioreactor is a device system that uses the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator, such as a fermenter, enzyme or cell reactor. It has important applications in wine, pharmaceutical production, concentrated jam, fruit juice fermentation, and degradation of organic pollutants.

Sampling operations are often required during biological cultivation in bioreactors. This operation usually needs to meet the requirements of sterility, control and quantification of sampling volume, and difference control of multiple sampling.

In prior art,

Such as the Chinese patent, patent application number: CN200520073106.0, which discloses a new type of fermentation automatic sampling valve, which is composed of a liquid inlet, a steam inlet, a transmission mechanism, a sampling port and a control valve. There is a servo motor with a controller. The steam inlet and sampling port are located on both sides of the sampling valve. One end is close to the liquid inlet and the other end is located at the lower part of the valve body. A sealing ring gasket is set on the interface disc surface at the liquid inlet on the top of the sampling valve. .

Also as: Chinese patent, patent application number: CN201020102452.8, which discloses a special sampling valve for a fermenter, including a fermenter, a first valve and a second valve, the inlet of the first valve communicates with the fermenter , the outlet of the first valve communicates with the inlet of the second valve, a sampling port is provided on the valve base of the first valve at its outlet, and a sampling valve is installed at the sampling port. The utility model has the advantages of: Simple, by installing a valve on the basis of the original valve, the structure of the valve on the valve is formed.

The common feature of the above two patents is a sampling device composed of pipes and valve components, which can automatically and directly take out the sample (as it is) inside the reactor, and has steam sterilization and sealing gaskets to prevent sampling contamination.

Such as Chinese patent, patent application number: CN200910034515.2 "on-line sugar concentration detection system during fermentation", the feature of this patent is that the constant flow pump directly takes samples from the fermentation tank (original), and then mixes the samples with pure water in proportion ( After dilution), it is sent to the detection instrument for determination. It uses a constant flow pump instead of sampling by the opening or closing of a valve. Avoid contamination of the reactor (fermenter) by the one-way flow of the liquid.

Such as the Chinese patent, patent application number: CN02138376.6 "a sampling and dilution device for an online detection system of a biological reaction process". For continuous filtration in the membrane element, a small amount of filtrate is drawn out from the pipeline (not in its original state), and after passing through a dilution device, it enters the detector for determination. The pore size of the ceramic membrane is between 0.1 and 0.2pm, and microorganisms cannot pass through it, which can effectively prevent the reactor (fermenter) from being polluted.

Such as US patent, patent number US4,942,770, Se1fert "a kind of aseptic sampling device". A three-way valve is connected to the reactor, and the sampling is completed by changing the valve path, that is, it is first connected to the fermenter, (positive) flows into the sampling pipeline, and then changes the valve (close the communication with the inside of the intoxication tank, open the connection with the external pipeline Unicom), the sample (original) in the sampling pipeline is reversely sent to the next dilution device or detection instrument by the liquid pump. Due to the switching of the three-way valve, the direct contact between the inside of the reactor and the outside world is avoided, thereby achieving the effect of pollution-free sampling.

Such as U.S. Patent, Patent No. US5,409,841, Chow "Sampling Device and Method for Ultraviolet Sterilization", adopts ultraviolet lamps to sterilize sampling pipes and containers, and does not use steam to sterilize.

For example, the US Patent No. US4,695,551, Samhaber, "Bioreactor Sampling Device", uses semi-permeable membrane permeation filtration for sampling. The semi-permeable membrane can isolate the passage of microorganisms to avoid contamination, and at the same time add a semi-permeable membrane vibration device to prevent the blockage of the membrane pores.

For example, the US Patent No. US5,914,092, Moon, "Steam Sterilization Device for Sampling Valve of Fermenter", relates to a device and method for improving the sterilization efficiency of steam for sampling valves and pipelines.

Such as U.S. Patent, Patent No. US6,860,162, Jaeger, "Liquid Sampling and Method", when sampling wine fermentation tanks, a sedimentation device is designed, and the sampling probe can avoid the sediment in the fermentation broth to prevent blockage.

To sum up, there are mainly two types of sampling operations in biological reactions in the prior art, one is separation membrane filtration, and the other is composed of pipelines and valves.

However, there are following defects and deficiencies in the above sampling operation method, as follows:

Separation membrane filtration sampling: Since the pore size of the membrane prevents the passage of bacteria, external pollution is avoided. However, this method is not suitable for the experimental requirements such as the detection of solid content in the culture medium, the detection of certain intracellular components, and the microscopic examination of organisms.

Sampling device consisting of pipes and valves: it can meet the needs of the above-mentioned experimental testing. There are also the following limitations:

1. Control methods to prevent contamination caused by sampling:

1. Environmental control plus disinfectant (alcohol) to disinfect the interface: high requirements for the use environment, and the disinfectant interferes with the sample (components, microscopic examination form, etc.);

2. Steam sterilizes pipelines and interfaces: steam needs to be configured during use, and the requirements for pipelines and experimental devices are complex (pressure resistance, scald prevention, steam discharge), and it is suitable for larger metal reaction devices;

3. Ultraviolet disinfection method: it brings complexity to the operation and has an inestimable impact on the sampling of active microorganisms.

4. Three-way switching valve method: Diaphragm valves are generally used for biological cultivation, and the three-way valve structure has dead angles, so it is impossible to evaluate the chance of pollution.

2. Control of waste liquid discharge in the sampling process:

During the sampling process, the remaining waste liquid in the pipeline needs to be removed first, so that the sampled liquid can be consistent with the culture liquid in the culture device. Microbial cultivation is a slow process, some take more than one day, some take many days. If there are many sampling times in the whole process and the total volume of the culture medium is limited, it is particularly important to control the discharge of residual waste liquid in the pipeline during each sampling.

It can be seen from the above that the sampling device in the biological culture process in the prior art cannot meet the requirements of strict sterility, control and quantification of sampling volume, and differential control of multiple sampling.

Contents of the invention

In view of this, the present invention aims to solve the technical problems that the sampling device in the biological culture process in the prior art cannot meet the requirements of strict sterility, control and quantification of sampling volume, and differential control of multiple sampling.

In order to solve the above problems, the present invention provides a sampling device for sampling the reactants in the bioreactor, comprising: a heating cooler; a gas pipeline passing through the heating cooler, one end connected to an air filter, The other end is an exhaust port; the first pinch valve is arranged on the gas pipeline, and is located between the air filter and the heating cooler; the second pinch valve is arranged on the gas pipeline, and Located between the heating cooler and the exhaust port; the sampling pipeline passes through the heating cooler, one end is located in the bioreactor, and the other end is a sample outlet; the first peristaltic pump is arranged on the sampling pipeline, And located between the bioreactor and the heating cooler; the second peristaltic pump is arranged on the sampling pipeline, and is located between the heating cooler and the sample outlet; wherein, the gas pipeline and the sampling pipeline pass through the The part of the heating cooler mentioned above is communicated.

Further, the part of the gas pipeline and the sampling pipeline passing through the heating cooler is the same pipeline.

Further, the sampling device also includes: a second heating cooler, arranged between the second pinch valve and the exhaust port; a third heating cooler, arranged between the second peristaltic pump and the sample between exits.

Further, the pipeline is a flexible silicone tube.

Further, the air filter is used for filtering air, and sterile air is passed into the pipeline.

Further, the sampling device also includes a controller, and the controller includes: an input and output device; a central controller connected to the input and output device; a multi-channel relay connected to the first pinch valve, the second Two pinch valves, a first peristaltic pump and a second peristaltic pump; a power module connected to the multi-channel relay.

Further, the central controller is a programmable logic controller.

Further, the input and output device is a touch screen.

The present invention also provides a bioreactor, which includes: a bioreactor or an analysis device, and any sampling device as described above connected to the bioreactor or analysis device.

In summary, the sampling device provided by the present invention and the biological reaction device with the sampling device can take out the living body under the premise of using sterile air to keep the pressure and ensuring the aseptic state by means of the actuators on the sampling pipeline. Biological analysis and microscopic examination, while avoiding the high temperature interference of biological forms.

Secondly, since sterile air is used to keep the pressure in the pipeline, the residual liquid in the rubber tube inserted into the sampled liquid can be blown back into the sampled liquid with sterile air through the reversal of the peristaltic pump, which avoids the residual liquid Interference with real-time sampling and analysis results also reduces the amount of residual waste liquid usually required to be discharged to zero; at the same time, relying on the pre-set time and flow rate of the peristaltic pump, the amount of each sample can be adjusted based on the accuracy of the peristaltic pump. Quantitative.

Finally, due to the sampling operation steps set by the program of the controller, the sampling operation can be combined with an external automatic analyzer for automatic sampling and online analysis, and the manual sampling mode can also be used for temporary sampling offline analysis.

Description of drawings

Figure 1 shows a schematic structural view of a sampling device provided by an embodiment of the present invention; Figure 2 shows a schematic structural view of a sampling device provided by an embodiment of the present invention;

Figure 3 shows a schematic structural view of a sampling device provided by another embodiment of the present invention;

Figure 4 shows a schematic structural view of a sampling device provided by another embodiment of the present invention;

Figure 5 shows a schematic structural view of a sampling device provided by another embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a sampling device provided by another embodiment of the present invention.

Detailed ways

In order to make the purpose and features of the present invention more comprehensible, the specific implementation manners of the present invention will be further described below in conjunction with the accompanying drawings.

Embodiment one

Please refer to FIG. 1 , which is a schematic structural diagram of a sampling device provided by an embodiment of the present invention.

The sampling device is used to sample the reactants in the bioreactor, including: a heating cooler 130; a gas pipeline 121 passing through the heating cooler 130, one end is connected to the air filter 110, and the other end is an exhaust gas mouth; the first pinch valve 141 is arranged on the gas pipeline, and is located between the air filter 110 and the heating cooler 130; the second pinch valve 142 is arranged on the gas pipeline, and is located Between the heating cooler 130 and the exhaust port; the sampling pipeline 122 passes through the heating cooler 130, one end is located in the bioreactor 100, and the other end is a sample outlet; the first peristaltic pump 151 is arranged on the On the sampling pipeline 122, and between the bioreactor 100 and the heating cooler 130; the second peristaltic pump 152, arranged on the sampling pipeline 122, and between the heating cooler and the sample outlet; wherein, the gas The pipe 121 communicates with the part of the sampling pipe 122 passing through the heating cooler 130 .

In the embodiment of the present invention, the part of the gas pipeline 121 and the sampling pipeline 122 passing through the heating cooler 130 is the same pipeline.

In the embodiment of the present invention, the gas pipeline 121 and the sampling pipeline 122 are flexible silicone tubes.

The specific steps of sampling using the sampling device provided by the embodiment of the present invention are as follows:

1. Control to prevent contamination caused by sampling:

1. All components of the device adopt the pinch method, such as: pinch valve, peristaltic pump, heating and cooler, so that the pinch valve, peristaltic pump, heating and cooler and other components have no direct contact with the sample.

2. The gas pipeline 121 and the sampling pipeline 122 adopt flexible silicone tubes, the gas pipeline 121 is equipped with an air filter 110, and the sampling pipeline 122 is connected to the culture device, such as the bioreactor 100, in place , the interface between the gas pipeline 121 and the sampling pipeline 122 is clamped or aseptically wrapped. Put it in an autoclave together with the culture device to sterilize before use. When in use, it only needs to be operated under the environmental conditions required for the cultivation process itself, and the pipeline is snapped into the pinch valve, peristaltic pump, and heating cooler. Since the second pinch valve 142 and the second peristaltic pump 152 have clamped the sampling pipeline 122, when the gas pipeline 121 is released and the sampling pipeline 122 is connected to the external interface at this time, the connection process will not affect the culture. liquid contamination.

3. The connection between the sampling pipeline 122 behind the second pinch valve 142 and the second peristaltic pump 152 and the external non-sterile pipeline may cause bacteria to pass through the second pinch valve 142 and the second peristaltic pump during long-term cultivation. The pump 152 causes pollution in the culture device, use sterile air (compressed air is filtered by the above-mentioned air filter) to keep the system aseptically pressurized, and open the second pinch valve 142 after sampling to remove the liquid adhering to the pipe wall. At the same time, according to the Pasteur disinfection principle, the heating cooler 130 is used to heat-insulate the sampling pipeline 122, so as to prevent the reverse propagation of bacteria along the pipeline and pollute the inside of the culture device. If the sample is taken out and passes through this high-temperature pipeline, it may affect the composition, the shape of the microscope, etc., and the temperature of the pipeline can be lowered by switching the heating mode to the cooling mode during sampling.

2. Control of waste liquid discharge in the sampling process:

1. Due to the adoption of the above-mentioned aseptic control measures, the aseptic state inside the pipeline is ensured. Before sampling, the first pinch valve 141 is opened, and the first peristaltic pump 151 is reversed, such as counterclockwise, to sample from the culture device. The residual liquid in the pipeline 122 is pumped back into the sampling device. Then the first peristaltic pump 151 and the second peristaltic pump 152 are rotated forward, such as clockwise, and the sample taken out at this time is an instant sample without discharging waste liquid.

2. According to the requirement of sampling volume, the sampling volume can be controlled by setting the running time of the peristaltic pump through the calibration of the peristaltic pump flow rate determined by the peristaltic pump speed, the inner diameter of the pipeline, and the pressure difference between the two ends of the peristaltic pump. When the required sample volume is reached, the first peristaltic pump 151 is reversed, such as counterclockwise, and the second peristaltic pump 152 continues to rotate forward, such as clockwise. At this time, with the access point of the sterile air pipeline as the demarcation point, the residual liquid near the end of the first peristaltic pump 151 is completely pressed into the culture device, and the sample taken near the end of the second peristaltic pump 152 completely flows into the external connection under the push of the sterile air. Sampling device or analyzer, the whole process will not produce oversampling and waste liquid.

In the embodiment of the present invention, referring to Fig. 2, the sampling device also includes a controller 200, and the controller 200 includes: an input and output device 210; a central controller 220, connected to the input and output device 210; a multi-way relay 230, respectively connected to the first pinch valve, the second pinch valve, the first peristaltic pump and the second peristaltic pump; the power module 240, connected to the multi-channel relay 230.

In the embodiment of the present invention, the central controller may be a programmable logic controller or a single-chip microcomputer.

In the embodiment of the present invention, the input and output device is a touch screen.

Based on the simple structure of the above-mentioned pipelines and execution devices, the controller is equipped with programmed control, which can achieve repetitive control of sampling time points, sampling process time, and sampling volume. Especially in the case of long-term and multiple sampling, it avoids the error caused by manual operation, and can also meet the use requirements when the total amount of culture medium is limited, thereby meeting the control requirements for the repeatability of sampling operations.

Embodiment two

Please refer to FIG. 3 , which is a schematic structural diagram of a sampling device provided by another embodiment of the present invention.

In this embodiment, the sampling device includes: a first heating cooler 331; a gas pipeline 321 passing through the first heating cooler 331, one end is connected to the air filter 310, and the other end is an exhaust port 323; the first pinch valve 341 is arranged on the gas pipeline, and is located between the air filter 310 and the first heating cooler 331; the second pinch valve 342 is arranged on the gas pipeline, And located between the first heating cooler 331 and the exhaust port 323; the sampling pipeline 322 passes through the first heating cooler 331, one end is located in the bioreactor 300, and the other end is a sample outlet 324; the first The peristaltic pump 351 is arranged on the sampling pipeline 322 and is located between the bioreactor 300 and the first heating cooler 331; the second peristaltic pump 352 is arranged on the sampling pipeline 322 and is located between the first heating and cooling Between the device 331 and the sample outlet 324; wherein, the gas pipeline 121 communicates with the part of the sampling pipeline 122 passing through the heating cooler 130. It also includes: a second heating cooler 332 arranged between the second pinch valve and the exhaust port; a third heating cooler 333 arranged between the second peristaltic pump 352 and the sample outlet.

In this embodiment, the heating coolers 331, 332, and 333 can be adjusted according to the distance from the analysis device, and a first heating cooler 331 can be used, or a pair of second and third heating coolers 332, 333 can be used. The temperature of the two pipes can be controlled separately, or the 332 and 333 can be combined into a dual-channel module. The present invention is not limited thereto.

Embodiment three

Please refer to FIG. 4 , which is a schematic structural diagram of a sampling device provided by another embodiment of the present invention.

Compared with the first embodiment, the peristaltic pump 151 in the first embodiment is replaced by the pinch valve 442 in the third embodiment, and accordingly the pumping power for the liquid in the reactor is provided by the syringe pump 451 instead.

The sampling actuator of the third embodiment is composed of a syringe pump 451, a peristaltic pump 452, pinch valves 441, 442, 443, a liquid storage tube 470, a heating cooler 431 or 432, 433, a flexible silicone tube and a pipeline connector, and then Combined with the control module to form the whole sampling device.

According to the required sampling accuracy, the syringe pump 451 can also be replaced by a peristaltic pump with lower accuracy (due to the different functions of the two, see process 3 and its description for specific programming steps).

Bind the emptying hose 422 and the sample hose 423 before use. Connect the liquid storage pipe 470, the bioreactor 400, the filter 410 and the following rubber hoses according to the figure, and then put them into an autoclave for sterilization. After the sterilization is completed, install the rubber hose into each actuator according to the diagram, and connect the inlet of the air filter with the air pipe 421.

The concrete flow process that adopts the sampling device that embodiment three provides to operate is as follows:

1. Drain the residual liquid from the sampling tube in the reactor:

Open the pinch valve 441 and pump air to a suitable volume with the peristaltic pump; close the pinch valve 441, open the pinch valve 442, press out the gas with the syringe pump 451, and press the remaining liquid in the reactor sampling tube back into the bioreactor. Turn on the cooling function of the heating cooler to reduce the temperature of the pipes in it.

2. Liquid pumping:

The syringe pump 451 draws air, and quantitatively pumps out the liquid in the reactor to the liquid storage pipe 470 according to the required amount.

3. Sample production:

Close the pinch valve 442; open the pinch valve 141, and at the same time, the peristaltic pump 452 rotates counterclockwise to press the liquid into the analysis device or sampling container.

4. Blow air:

Turn off the peristaltic pump 452; open the pinch valve 443 and close it quickly to blow out a small amount of liquid adhering to the tube wall, so as to reduce the interference to the next sampling. Turn on the heating function of the heating cooler.

5. Press back the residual liquid in the sampling tube:

Repeat step 1.

Step 1 and step 5 are repeated, and the functions are the same. Only step 1 is required for the first sampling, and each subsequent operation is just a cycle of steps 2-5.

Embodiment Four

Please refer to FIG. 5 , which is a schematic structural diagram of a sampling device provided by another embodiment of the present invention.

Compared with the third embodiment, the main difference is that the syringe pump 451 is replaced by the peristaltic pump 551 in the fourth embodiment.

Runtime is executed in the following steps:

1. Drain the residual liquid from the sampling tube in the reactor:

Open the pinch valve 542, and at the same time, the peristaltic pump 551 rotates counterclockwise, and press the participating liquid in the reactor sampling tube back into the reactor with sterile air. Turn on the cooling function of the heating cooler to reduce the temperature of the pipes in it.

2. Liquid pumping:

The peristaltic pump 551 rotates clockwise to quantitatively pump out the liquid in the reactor to the liquid storage pipe 170 according to the required amount.

3. Sample production:

Close the pinch valve 542; open the pinch valve 541, and at the same time, the peristaltic pump 552 rotates counterclockwise to press the liquid into the analysis device or sampling container.

4. Blow air:

Turn off the peristaltic pump 552; open the pinch valve 543 and close it quickly to blow out a small amount of liquid adhering to the tube wall, so as to reduce the interference to the next sampling. Turn on the heating function of the heating cooler.

5. Press back the residual liquid in the sampling tube:

Repeat step 1.

Step 1 and step 5 are repeated, and the functions are the same. Only step 1 is required for the first sampling, and each subsequent operation is just a cycle of steps 2-5.

For the above-mentioned embodiments two to four, variations and combinations can also be made, including but not limited to:

According to the volume of the sample volume, determine the volume of the liquid storage tube, or directly replace the volume of the liquid storage tube by the length of the pipeline.

According to the distance between the sampling device and the analysis device, the heating and cooling unit can be independent, or it can be installed separately or combined into a dual-channel heating and cooling module in the exhaust pipe and sample outlet pipe.

According to specific operating requirements, the pinch valve can be replaced by a peristaltic pump, and the peristaltic pump can also be replaced by a pinch valve.

Replace the pinch valve and the pinch valve with a peristaltic pump, and the program control steps remain unchanged, only involving the switch control of the pinch valve and the rotation control (including the rotation direction control) of the peristaltic pump.

Embodiment five

Please refer to FIG. 6 , which is a schematic structural diagram of a sampling device provided by another embodiment of the present invention.

In this embodiment, in addition to the application method of combining the sampling device with the reactor in the embodiment and then sterilizing it in an autoclave, the connecting pipe of the device and the reactor can also be connected by a diaphragm valve way, see Figure 6.

Diaphragm valves 682, 683 are connected by welding. Before use, the diaphragm valve 682 is closed and connected to the rubber hose, air filter 610, and liquid storage pipe 670. After the exhaust pipe 623 and the sample outlet pipe 624 are wrapped, they are put into an autoclave for sterilization.

When in use, connect the diaphragm valve 682 to the interface of the reactor sampling valve 684, and adopt the conventional secondary steam sterilization connection method of the bioreactor to realize the aseptic connection between the bioreactor and the sampling device (no more details here) .

After aseptic connection, the diaphragm valve 682 is opened, and the diaphragm valves 681 and 683 are closed, and the subsequent automatic sampling program control steps are consistent with the aforementioned procedures and steps. Diaphragm valves 681-683 and sampling valve 684 can be manual valves or automatic control valves, and their automatic control programs can also be integrated into the sampling controller.

In addition, an embodiment of the present invention also provides a bioreactor, which includes: a bioreactor or an analysis device, and any sampling device as provided in the above embodiments connected to the bioreactor or analysis device.

For example: the whole set of bioreactor can be combined with bioreactor, can also be used in combination with analysis device, or a combination of all three. Its specific structural principles are as above, and will not be repeated here.

To sum up, the sampling device provided by the embodiment of the present invention and the biological reaction device with the sampling device, first of all, on the premise of using sterile air to keep the pressure and using the actuators on the sampling pipeline to ensure the aseptic state, Living organisms can be taken out for analysis and microscopic examination, while avoiding the high temperature interference of biological forms.

Secondly, since sterile air is used to keep the pressure in the pipeline, the residual liquid in the rubber tube inserted into the sampled liquid can be blown back into the sampled liquid with sterile air through the reversal of the peristaltic pump, which avoids the residual liquid Interference with real-time sampling and analysis results also reduces the amount of residual waste liquid usually required to be discharged to zero; at the same time, relying on the pre-set time and flow rate of the peristaltic pump, the amount of each sample can be adjusted based on the accuracy of the peristaltic pump. Quantitative.

Finally, due to the sampling operation steps set by the program of the controller, the sampling operation can be combined with an external automatic analyzer for automatic sampling and online analysis, and the manual sampling mode can also be used for temporary sampling offline analysis.

The sampling device provided by the invention satisfies the requirements of sterility, control and quantification of sampling volume, difference control of multiple sampling, and the like.

Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Any person with ordinary knowledge in the technical field may make various changes without departing from the spirit and scope of the present invention. and retouching, so the scope of protection of the present invention should be defined by the claims.

Claims (9)

1. a sampler, for sampling the reactant in bio-reactor, is characterized in that, comprising:

Heating refrigeratory;

Gas pipeline, through described heating refrigeratory, one end is connected to air strainer, and the other end is exhausr port;

First pinch valve, is arranged on described gas pipeline, and between described air strainer and the first heating refrigeratory;

Second pinch valve, is arranged on described gas pipeline, and between described first heating refrigeratory and exhausr port;

Sampling pipe, through described first heating refrigeratory, one end is arranged in bio-reactor, and the other end is sample export;

First peristaltic pump, is arranged on described sampling pipe, and between bio-reactor and the first heating refrigeratory;

Second peristaltic pump, is arranged on described sampling pipe, and between the first heating refrigeratory and sample export;

Wherein, described gas pipeline communicates through the described first part heating refrigeratory with described sampling pipe.

2. sampler according to claim 1, is characterized in that, the part that described gas pipeline and described sampling pipe pass described heating refrigeratory is same pipeline.

3. sampler according to claim 1, is characterized in that, also comprises: the second heating refrigeratory, is arranged between described second pinch valve and exhausr port;

3rd heating refrigeratory, is arranged between described second peristaltic pump and sample export.

4. sampler according to claim 1, is characterized in that, described pipeline is flexible silicone tube.

5. sampler according to claim 1, is characterized in that, described air strainer is used for filtering air, passes into filtrated air to described pipeline.

6. sampler according to claim 1, is characterized in that, also comprises: controller, and described controller comprises:

Input-output unit;

Central controller, is connected with described input-output unit;

Multicircuit relay, is connected to described first pinch valve, the second pinch valve, the first peristaltic pump and the second peristaltic pump;

Power module, is connected with described multicircuit relay.

7. sampler according to claim 6, is characterized in that, described central controller is programmable logic controller (PLC).

8. sampler according to claim 6, is characterized in that, described input-output unit is touch-screen.

9. a biological reaction apparatus, is characterized in that, comprising: bio-reactor or analytical equipment, and be connected with described bio-reactor or analytical equipment as sampler arbitrary in claim 1 to 8.