CN220976982U - Quantitative sterile sampling device of bioreactor - Google Patents
Quantitative sterile sampling device of bioreactor Download PDFInfo
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- CN220976982U CN220976982U CN202322712784.8U CN202322712784U CN220976982U CN 220976982 U CN220976982 U CN 220976982U CN 202322712784 U CN202322712784 U CN 202322712784U CN 220976982 U CN220976982 U CN 220976982U
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- pipeline
- sampling
- sample
- bottle
- bioreactor
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- 238000005070 sampling Methods 0.000 title claims abstract description 88
- 230000001954 sterilising effect Effects 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 31
- 238000002347 injection Methods 0.000 claims description 24
- 239000007924 injection Substances 0.000 claims description 24
- 238000004891 communication Methods 0.000 claims description 6
- 230000036512 infertility Effects 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 8
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000003749 cleanliness Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a quantitative sterile sampling device of a bioreactor, which structurally comprises a sampling bottle, wherein the sampling bottle is connected with a sampling pipeline, a sampling outlet pipeline and a gas pipeline, the end part of the sampling pipeline is detachably connected with a first steam sterilizing pipeline and an external pipe used for being communicated with the bioreactor, the external pipe is provided with a liquid flowmeter, the external pipe is communicated with a second steam sterilizing pipeline, ports of the sampling outlet pipeline and the gas pipeline are respectively provided with a gas filter, and the sampling outlet pipeline extends into the sampling bottle to the bottom of the sampling bottle. After the utility model is used, the sampling operation in the sampling process of the bioreactor is not limited by the environmental clean grade, the problem of sterility of the sample can be ensured, and the quantitative sampling operation can be carried out.
Description
Technical Field
The utility model relates to a reactor sampling device, in particular to a quantitative sterile sampling device for a bioreactor.
Background
In the process of culturing and fermenting cells or microorganism bioreactor, the control parameters of the reactor culture are adjusted by judging the thallus state after sampling and detecting the physical and chemical indexes of the growth environment, so that the yield of the target protein of microorganism culture can be obviously improved.
The accurate detection mode of the cell state and the physicochemical index is mainly detection after offline sampling. The current sampling mode is to connect the bioreactor with the sampling bottle through a pipeline, so that the liquid in the bioreactor enters the sampling bottle, and the sampling bottle and the internal liquid are sent to the subsequent detection. During sampling, the clean level of the environment must be C level and above, and the components such as the sampling bottle and the pipeline must be sterilized before sampling. On the one hand, this way is limited by the clean level of the environment, and the whole sampling process and the transferring process of the sampling bottle must be carried out in a clean environment; on the other hand, it is not guaranteed that all connecting lines and components are sterilized, and sterility of each component during transfer is not guaranteed.
In the current bioreactor sampling mode, the sampling operation is limited by the environmental clean grade, and the sterility of the sample cannot be ensured.
Disclosure of utility model
The utility model aims to provide a quantitative sterile sampling device for a bioreactor, which solves the problems that the sampling operation of the existing bioreactor is limited by the environmental clean grade and the sterility of a sample cannot be ensured.
The utility model is realized in the following way: the utility model provides a including the sample bottle, the sample bottle is connected with sampling pipeline, goes out appearance pipeline and gas pipeline, sampling pipeline tip can dismantle be connected with first steam disinfection pipeline and be used for with the external pipe of bioreactor intercommunication be provided with the liquid flowmeter on the external pipe, and the intercommunication has the second steam disinfection pipeline on the external pipe go out the port of appearance pipeline and gas pipeline is provided with gas filter respectively, go out the bottom that the appearance pipeline extends to the sample bottle in to the sample bottle.
The sample injection pipeline is connected with the external connection pipe and the sample injection pipeline is connected with the first steam sterilization pipeline through a manual valve.
The sample injection pipeline is provided with a first valve, and the outer connecting pipe is provided with a second valve.
The sampling bottle comprises a bottle body and a bottle cap, wherein a plurality of communication ports are formed in the bottle cap, and each communication port is respectively communicated with the sample injection pipeline, the sample outlet pipeline and the gas pipeline.
And a quick connector is arranged on the outlet pipe.
The sample outlet pipeline and the gas pipeline are hoses, and the hoses are provided with pipe clamps.
At least one of the gas pipelines is arranged.
The external connection pipe part is used for being communicated with the bioreactor, the sampling bottle, the sampling pipeline, the sampling outlet pipeline and the gas pipeline form a sampler part, the sampler part and the external connection pipe part are detachably connected, the sampler part can be independently sterilized, the sampler part can be independently formed into a sealing part, the sealing state can be kept in the transferring process after sterilization or sampling, and the pollution of the external environment to the sample is avoided. Meanwhile, the sample injection pipeline is detachably connected with the first steam sterilization pipeline, and after the sample injection pipeline is communicated with the external connection pipe and the first steam sterilization pipeline, the external connection pipe is sterilized through the first steam sterilization pipeline and the second steam sterilization pipeline, so that the sterility of the whole device is guaranteed. The sterility of the whole device can be ensured, and the sampler part can form an independent sterile sealing structure, so that after a sample enters the sampling bottle, the sampler part can be taken to the biosafety cabinet for subsequent sterile operation, and the sampling process is not limited by the cleanliness of the environment.
Meanwhile, the liquid flowmeter is arranged on the outer connecting pipe, so that the quantitative sampling of the sample can be realized.
Drawings
Fig. 1 is a structural diagram of the present utility model.
In the figure: 1. sampling bottle; 2. a sample injection pipeline; 3. a sample outlet pipeline; 4. a gas line; 5. a first steam sterilization line; 6. an outer connecting pipe; 7. a second steam sterilization line; 8. a liquid flow meter; 9. a quick connector; 10. a gas filter; 11. a first valve; 12. a second valve; 13. a manual valve; 14. a pipe clamp; 1-1, a bottle body; 1-2, bottle cap.
Detailed Description
As shown in fig. 1, the utility model comprises a sampling bottle 1, wherein the sampling bottle 1 is connected with a sample inlet pipeline 2, a sample outlet pipeline 3 and a gas pipeline 4. The sampling bottle 1, the sampling pipeline 2, the sampling pipeline 3 and the gas pipeline 4 form a sampler part together. The end part of the sample injection pipeline 2 is detachably connected with a first steam sterilization pipeline 5 and an external pipe 6 which is used for being communicated with a bioreactor, a liquid flowmeter 8 is arranged on the external pipe 6, and a second steam sterilization pipeline 7 is communicated with the external pipe 6.
The outer connecting pipe 6 is communicated with a sampling port of the bioreactor, and liquid of the bioreactor flows into the sampling pipeline 2 through the outer connecting pipe 6 and enters the sampling bottle 1 through the sampling pipeline 2.
In order to sterilize the outer connecting pipe 6, a second steam sterilization pipeline 7 is communicated with the outer connecting pipe 6, and a first steam sterilization pipeline 5 is communicated with the sample injection pipeline 2. High-temperature steam is input from one of the first steam sterilizing pipeline 5 or the second steam sterilizing pipeline 7, high-temperature steam is output from the other, and the high-temperature steam passes through the outer connecting pipe 6 part, so that the outer connecting pipe 6 is sterilized at high temperature.
The first steam sterilizing pipeline 5 and the second steam sterilizing pipeline 7 are communicated with a steam pipeline system, the external pipe 6 is communicated with the bioreactor, and the part is generally a fixed part, so that the sample injection pipeline 2 is communicated with the external pipe 6 and the first steam sterilizing pipeline 5 through the manual valve 13, the manual valve 13 can be manually closed or opened, and the quick detachable connection with the external pipe 6 or the first steam sterilizing pipeline 5 can be realized through a quick connection structure of a port of the manual valve 13.
The sample injection pipeline 2 is provided with a first valve 11, and the external pipe 6 is provided with a second valve 12.
The first valve 11 is a three-way valve, one port of the first valve is communicated with the sample injection pipeline 2, the other two ports are respectively communicated with the manual valve 13, and the two manual valves 13 are respectively communicated with the external pipe 6 and the first steam sterilization pipeline 5.
The first valve 11 is used for controlling liquid to enter the sampler through the sample injection pipeline 2 or steam to pass through the first steam sterilizing pipeline 5, the external connecting pipe 6 and the second steam pipeline.
The second valve 12 is used for sealing the outer connecting pipe 6 when the sample injection pipeline 2 is disconnected from the outer connecting pipe 6.
The gas filters 10 are provided at the ports of the sample outlet pipe 3 and the gas pipe 4, respectively, and the sample outlet pipe 3 extends into the sample bottle 1 to the bottom of the sample bottle 1.
The gas is filtered by the gas filter 10 to ensure sterility of the gas entering the sample bottle 1.
The sampling pipeline 3 extends to the bottom of the sampling bottle 1 towards the inside of the sampling bottle 1, when a liquid sample is stored in the sampling bottle 1, the gas pipeline 4 is connected with a gas injection device, gas is injected into the sampling bottle 1, the gas filter 10 of the sampling pipeline 3 is taken down, the liquid in the sampling bottle 1 is discharged along the sampling pipeline 3 under the action of gas pressure, and the liquid sample can be taken out from the sampling bottle 1.
The sample injection pipeline 2 is detachably connected with the external connection pipe 6 and the sample injection pipeline 2 is detachably connected with the first steam sterilization pipeline 5, so that the sampler part can be moved, the sampler part can be placed into a high-pressure steam sterilization pot for sterilization, and after sterilization is completed, the sampler part is connected with the external connection pipe 6 and the first steam sterilization pipeline 5 through a manual valve 13 made of stainless steel.
The sampling bottle 1 comprises a bottle body 1-1 and a bottle cap 1-2, the bottle cap 1-2 is in sealing connection with the bottle body 1-1, a plurality of communication ports are formed in the bottle cap 1-2, and each communication port is respectively communicated with a sample injection pipeline 2, a sample discharge pipeline 3 and a gas pipeline 4.
Wherein at least one gas line 4 is provided. Two gas lines 4 may be provided, and when one gas line 4 is blocked, the gas in the bottle 1-1 may be discharged through the spare gas line 4.
The quick connector 9 is arranged on the sample outlet pipeline 3, and the quick connector 9 can realize quick installation or disassembly of the gas filter 10 on the sample outlet pipeline 3.
The sample outlet line 3 and the gas line 4 are hoses, and a pipe clamp 14 is provided on each hose. The connection or disconnection of the sample outlet line 3 and the gas line 4 is controlled by the pipe clamp 14.
When the device is used, firstly, the sampler part is placed in a high-pressure steam sterilizing pot, high-temperature sterilization is carried out for 15min at 121 ℃, after sterilization is finished, the sample injection pipeline 2 is connected with the first steam sterilizing pipeline 5 and the external connecting pipe 6 through the manual valve 13, steam is opened, and the external connecting pipe 6 is sterilized through the first steam sterilizing pipeline 5 and the second steam sterilizing pipeline 7, so that the whole device and the pipeline are sterilized; when sampling in the fermentation process, quantitative sterile sampling is realized by arranging a liquid flowmeter 8 and opening a manual sampling valve, the sampled product flows into a glass sampling bottle 1, the manual sampling valve is closed after sampling is finished, the stainless steel manual valve is disassembled, and a sterile sampler is taken to a biological safety cabinet for subsequent sterile operation; because the sterile sampler is provided with the four-way interface, the bottle cap 1-2 can be not opened in the biosafety cabinet to sample, the pipe clamp 14 on one gas pipeline 4 is clamped, the gas inlet end of the gas filter 10 on the other gas pipeline 4 is connected with the gas injection device, the gas injection device can be a pedal type inflator with a silicone pipe, the quick connector 9 on the sample outlet pipeline 3 is separated, and the sample in the bottle can be taken out by stepping the pedal type inflator; because of the non-timing of sampling in the fermentation process, two sets of sampler parts are provided, and the sampler parts are alternately used by high-pressure sterilization in the fermentation process, so that the requirement of frequent sampling in the fermentation process is met, and quantitative sterile sampling is realized.
The external connection pipe 6 part is used for being communicated with a bioreactor, the sampling bottle 1, the sampling pipeline 2, the sampling outlet pipeline 3 and the gas pipeline 4 form a sampler part, the sampler part and the external connection pipe 6 part are detachably connected, the sampler part can be independently sterilized, the sampler part can be independently formed into a sealing part, the sealing state can be kept in the transferring process after sterilization or sampling, and the pollution of the external environment to the sample is avoided. Meanwhile, the sample injection pipeline 2 is detachably connected with the first steam sterilization pipeline 5, and after the sample injection pipeline 2 is communicated with the external connection pipe 6 and the first steam sterilization pipeline 5, the external connection pipe 6 is partially sterilized through the first steam sterilization pipeline 5 and the second steam sterilization pipeline 7, so that the sterility of the whole device is guaranteed. The sterility of the whole device can be ensured, and the sampler part can form an independent sterile sealing structure, so that after a sample enters the sampling bottle 1, the sampler part can be taken to a biosafety cabinet for subsequent sterile operation, and the sampling process is not limited by the cleanliness of the environment. Meanwhile, the liquid flowmeter 8 is arranged on the outer connecting pipe 6, so that the quantitative sampling of the sample can be realized.
Claims (7)
1. The utility model provides a sterile sampling device of bioreactor ration, its characterized in that, includes the sample bottle, the sample bottle is connected with sampling pipeline, goes out appearance pipeline and gas pipeline, sampling pipeline tip can dismantle be connected with first steam disinfection pipeline and be used for with the outer pipe of bioreactor intercommunication be provided with liquid flowmeter on the outer take over, and the intercommunication has the second steam disinfection pipeline on the outer take over go out the port of appearance pipeline and gas pipeline is provided with gas filter respectively, go out appearance pipeline to the bottom of sample bottle that extends into in the sample bottle.
2. The quantitative sterile sampling device for a bioreactor according to claim 1, wherein the sample introduction pipeline and the external connection pipe and the sample introduction pipeline and the first steam sterilization pipeline are connected through manual valves.
3. The quantitative sterile sampling device for a bioreactor according to claim 1, wherein a first valve is provided on the sample introduction line and a second valve is provided on the extension tube.
4. The quantitative sterile sampling device for a bioreactor according to claim 1, wherein the sampling bottle comprises a bottle body and a bottle cap, a plurality of communication ports are formed in the bottle cap, and each communication port is respectively communicated with a sample injection pipeline, a sample discharge pipeline and a gas pipeline.
5. The quantitative sterile sampling device for a bioreactor according to claim 1, wherein a quick connector is provided on the outlet tube.
6. The quantitative sterile sampling device for a bioreactor according to claim 1, wherein the sample outlet pipeline and the gas pipeline are hoses, and a pipe clamp is arranged on the hoses.
7. The bioreactor quantitative sterile sampling device of claim 1 wherein at least one of said gas lines is provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322712784.8U CN220976982U (en) | 2023-10-10 | 2023-10-10 | Quantitative sterile sampling device of bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322712784.8U CN220976982U (en) | 2023-10-10 | 2023-10-10 | Quantitative sterile sampling device of bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220976982U true CN220976982U (en) | 2024-05-17 |
Family
ID=91060749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322712784.8U Active CN220976982U (en) | 2023-10-10 | 2023-10-10 | Quantitative sterile sampling device of bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220976982U (en) |
-
2023
- 2023-10-10 CN CN202322712784.8U patent/CN220976982U/en active Active
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