CN205933818U - A tee bend pipeline connected system for bioreactor sample - Google Patents

Abstract

The utility model discloses a tee bend pipeline connected system for bioreactor sample, including first silicone tube, second silicone tube and third silicone tube, to connect through tee bend pipe fittings between first silicone tube, second silicone tube and the third silicone tube, first silicone tube passes through the leather hose joint and the clamp is continuous with the sample valve on the bioreactor, be equipped with the liquid end of taking a sample out on the third silicone tube, the liquid of taking a sample out end connects for glass, and glass connects including glass plug and the female seat of glass, and the female seat of glass links to each other with the flow stopping pipe. The utility model discloses the liquid of liquid end of taking a sample out of can discharging after the sample, pollution abatement risk in the operation prevents that intraductal liquid dropping from influence operation environment, the longer cell culture of qieyu in the cycle pollution risk hang down to firing disinfection to glass connects all can reduce in the cycle because of the bioreactor pollution risk that sampling operation exists at a longer cell culture, and liquid dropping leads to the fact harmful effects to operation environment in also can avoiding connecting.

Description

A kind of three-way pipeline connection system for bioreactor sampling

Technical field

This utility model is related to the technical field of bio-pharmaceuticals, more particularly, to a kind of threeway for bioreactor sampling Plumbing connection system.

Background technology

Bioreactor technology is the important technology of INVENTIONModern cell culture, is characterized in efficiently convenient, bioreactor is Cell one homogeneous, airtight, growing environment that each parameter is controlled is provided it is adaptable to small-scale test to pilot scale transition, Nai Zhifang Greatly produce in enormous quantities.

In cell cultivation process in order to detect, understand cell growth condition it is necessary to from culture fluid obtain sample in order to Detection.In common sampling mode, probe tube liquid outlet/mouth of pipe can be full of culture fluid, easily drip, have necessarily not during jointing Just, operating environment such as superclean bench etc. is easily caused with dirty, pollution, there is also the risk making bioreactor pollute.

Utility model content

For the deficiencies in the prior art, technical problem to be solved in the utility model is to overcome usual sampling mode Deficiency, provides a kind of three-way pipeline connection system for bioreactor sampling, reduces operation, the risk of pollution, and relatively Its low stain risk can be ensured in long cell cultivation cycle.

In order to solve above-mentioned technical problem, this utility model is achieved through the following technical solutions：A kind of anti-for biology Answer the three-way pipeline connection system of device sampling, including the first silica gel tube, the second silica gel tube and the 3rd silica gel tube, described first silica gel Connected by threeway pipe connections between pipe, the second silica gel tube and the 3rd silica gel tube, described first silica gel tube passes through leather pipe joint It is connected with the sampling valve on bioreactor with clip；Described 3rd silica gel tube is provided with sampling liquid outlet, described samples out liquid Hold as glass connector, described glass connector includes glass plug and glass female seat, and described glass female seat is connected with fluid stopping pipe.

Further, described first silica gel tube, the second silica gel tube and the 3rd silica gel tube are equipped with and control liquid flowing Vessel clamp.

Of the present utility model one preferred embodiment in, described fluid stopping pipe is provided with strip flow-stopping clip.

Further, the end of described second silica gel tube is provided with the first air filter.

As the improvement of such scheme, the end of described fluid stopping pipe is provided with the second air filter.

As the improvement of such scheme, described second silica gel tube and the first air filter hang on eminence.

With respect to prior art, the beneficial effects of the utility model are：Of the present utility model for bioreactor sampling Three-way pipeline connection system by threeway pipe connections connect three sections of silica gel tubes form, wherein one end connect bioreactor, one Hold for sampling out liquid, one end is located at higher position, can be connected with outside air by air filter, three segment pipes are by conduit Folder controls liquor stream.After sampling, available vessel clamp clamps the first silica gel tube connecting with bioreactor, opens and is connected with air Vessel clamp on second silica gel tube, can discharge liquid in the pipeline of sampling liquid outlet.This utility model can be arranged after sampling Go out to sample the liquid of liquid outlet, reduce pollution risk in operation, prevent liquid in pipe drippage impact operating environment, and in longer Cell culture period in pollution risk low, compared with common sampling pipe, this utility model, after sampling terminates, can empty The glass connector of sampling liquid outlet and liquid in the pipeline of place, facilitate the calcination of glass connector to sterilize, in a longer cell The bioreactor pollution risk existing because of sampling operation all can be reduced in cultivation cycle, liquid drippage in joint also can be avoided right Operating environment (as superclean bench etc.) has undesirable effect, and is worthy of popularization.

Brief description

In order to be illustrated more clearly that the technical scheme of this utility model embodiment, simple by making to the accompanying drawing of embodiment below Ground is introduced.

Fig. 1 is the structure of the three-way pipeline connection system for bioreactor sampling of this utility model preferred embodiment Schematic diagram.

Fig. 2 is the sampling of the three-way pipeline connection system for bioreactor sampling of this utility model preferred embodiment The structural representation of liquid outlet.

Specific embodiment

Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out Clearly and completely description is it is clear that described embodiment is only a part of embodiment of this utility model rather than whole Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of making creative work The every other embodiment being obtained, broadly falls into the scope of this utility model protection.

As depicted in figs. 1 and 2, the three-way pipeline for bioreactor sampling of this utility model preferred embodiment connects The structural representation of system, the three-way pipeline connection system for bioreactor sampling of the present utility model includes the first silica gel Pipe 10, the second silica gel tube 20 and the 3rd silica gel tube 30, described first silica gel tube 10, the second silica gel tube 20 and the 3rd silica gel tube 30 it Between connected by threeway pipe connections 40, described first silica gel tube 10 passes through on leather pipe joint and clip 11 and bioreactor 50 Sampling valve 51 be connected；Described 3rd silica gel tube 30 is provided with sampling liquid outlet, and described sampling liquid outlet is glass connector 60, institute State glass connector 60 and include glass plug 61 and glass female seat 62, described glass female seat 62 is connected with fluid stopping pipe 70.This practicality is new The vessel clamp 80 controlling liquid flowing is equipped with the first silica gel tube 10 in type, the second silica gel tube 20 and the 3rd silica gel tube 30.

Preferably, described fluid stopping pipe 70 is provided with strip flow-stopping clip 71.

In this utility model, the end of described second silica gel tube 20 is provided with the first air filter 21, described fluid stopping pipe 70 end is provided with the second air filter 72, is used for completely cutting off outward using the first air filter 21 and the second air filter 72 Portion's environmental pollution is it is ensured that aseptic condition within tubing.

In addition, described second silica gel tube 20 and the first air filter 21 hang on eminence.

Below by being specifically described in more detail to this utility model using process：

Using front, plumbing connection system of the present utility model and bioreactor 50, all through autoclaving, are aseptic State.Vessel clamp 80 on first silica gel tube 10, the second silica gel tube 20 and the 3rd silica gel tube 30 should be at when no operating closing State, glass connector 60 should be docking the state being fixed with rubber band when no operating.It is first turned on sampling valve during use 51, in superclean bench, untie the rubber band on glass connector 60, above alcohol burner flame, glass connector 60 calcination is disappeared It is split up into glass plug 61 and glass female seat 62 after poison, glass plug 61 is directed at waste liquid bottle bottleneck, prepare discharge opeing.First beat successively Open the vessel clamp 80 on the first silica gel tube 10 and the 3rd silica gel tube 30, in bioreactor 50 liquid due to container intrinsic pressure by One silica gel tube 10 and the 3rd silica gel tube 30 are to discharge opeing in waste liquid bottle, until the liquid flowing out in bioreactor 50 uniformly, closes Close the vessel clamp 80 on the 3rd silica gel tube 30.Glass plug 61 is directed at sampling container mouth, opens leading on the 3rd silica gel tube 30 Pipe clamp 80 is sampled, and closes the vessel clamp 80 on the 3rd silica gel tube 30 and the first silica gel tube 10 after terminating successively.Movable glass Plug 61 be aligned waste liquid bottleneck, open the vessel clamp 80 on the first silica gel tube 10 and the second silica gel tube 20 successively, liquid in pipe by It is discharged in atmospheric pressure.Empty liquid in the 3rd silica gel tube 30, after terminating, close the 3rd silica gel tube 30 and the second silica gel successively Vessel clamp 80 on pipe 20.On alcohol burner, calcination glass plug 61 and glass female seat 62, are tied up with rubber band to after connecting.Bar Shape flow-stopping clip 71 can stop a small amount of liquid that may remain in glass connector 60 from drenching pollution the second air filter 72.

Three-way pipeline connection system for bioreactor sampling of the present utility model is connected by threeway pipe connections 40 Three sections of silica gel tubes (the first silica gel tube 10, the second silica gel tube 20 and the 3rd silica gel tube 30) form, and wherein one end connects biological respinse Device 50, one end is used for sampling out liquid, and one end is located at higher position, can be connected with outside air by air filter, three segment pipes Control liquor stream by vessel clamp 80.After sampling, available vessel clamp 80 clamps the first silica gel tube 10 connecting with bioreactor 50, Open the vessel clamp 80 on the second silica gel tube 20 being connected with air, liquid in the pipeline of sampling liquid outlet can be discharged.This reality With the new liquid that can discharge sampling liquid outlet after sampling, reduce pollution risk in operation, prevent liquid in pipe from dripping Impact operating environment, and pollution risk is low in longer cell culture period, compared with common sampling pipe, this practicality is new Type, after sampling terminates, can empty glass connector 60 and the liquid in the pipeline of place of sampling liquid outlet, facilitate glass connector 60 Calcination is sterilized, and all can reduce the bioreactor pollution wind existing because of sampling operation in a longer cell culture period Danger, also can avoid liquid drippage in joint that operating environment (as superclean bench etc.) is had undesirable effect, be worthy of popularization.

Above disclosed only preferred embodiment of the present utility model, can not limit this practicality with this new certainly The interest field of type, the equivalent variations therefore made according to this utility model claim, still belong to the model that this utility model is covered Enclose.

Claims (6)

1. a kind of three-way pipeline connection system for bioreactor sampling it is characterised in that：Including the first silica gel tube, second Silica gel tube and the 3rd silica gel tube, pass through threeway pipe connections between described first silica gel tube, the second silica gel tube and the 3rd silica gel tube Connect, described first silica gel tube is connected with the sampling valve on bioreactor with clip by leather pipe joint；Described 3rd silica gel Pipe is provided with sampling liquid outlet, and described sampling liquid outlet is glass connector, and described glass connector includes glass plug and glass is female Seat, described glass female seat is connected with fluid stopping pipe.

2. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described The vessel clamp controlling liquid flowing is equipped with one silica gel tube, the second silica gel tube and the 3rd silica gel tube.

3. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described only Flow tube is provided with strip flow-stopping clip.

4. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described The end of second silica gel pipe is provided with the first air filter.

5. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described only The end of flow tube is provided with the second air filter.

6. be used for as claimed in claim 4 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described Second silica gel pipe and the first air filter hang on eminence.