CN205933818U - A tee bend pipeline connected system for bioreactor sample - Google Patents

Abstract

The utility model discloses a tee bend pipeline connected system for bioreactor sample, including first silicone tube, second silicone tube and third silicone tube, to connect through tee bend pipe fittings between first silicone tube, second silicone tube and the third silicone tube, first silicone tube passes through the leather hose joint and the clamp is continuous with the sample valve on the bioreactor, be equipped with the liquid end of taking a sample out on the third silicone tube, the liquid of taking a sample out end connects for glass, and glass connects including glass plug and the female seat of glass, and the female seat of glass links to each other with the flow stopping pipe. The utility model discloses the liquid of liquid end of taking a sample out of can discharging after the sample, pollution abatement risk in the operation prevents that intraductal liquid dropping from influence operation environment, the longer cell culture of qieyu in the cycle pollution risk hang down to firing disinfection to glass connects all can reduce in the cycle because of the bioreactor pollution risk that sampling operation exists at a longer cell culture, and liquid dropping leads to the fact harmful effects to operation environment in also can avoiding connecting.

Description

A three-way pipe connection system for bioreactor sampling

technical field

The utility model relates to the technical field of biopharmaceuticals, in particular to a three-way pipe connection system used for bioreactor sampling.

Background technique

Bioreactor technology is an important technology for modern cell culture. It is characterized by high efficiency and convenience. Bioreactors provide cells with a uniform, airtight, and controllable growth environment. Mass production.

In order to detect and understand the growth status of cells during cell culture, samples must be obtained from the culture medium for detection. In the usual sampling method, the liquid outlet/mouth of the sampling tube will be filled with culture solution, which is easy to drip, and it is inconvenient to connect the joints. Risk of reactor contamination.

Utility model content

Aiming at the deficiencies of the existing technology, the technical problem to be solved by the utility model is to overcome the deficiencies of the usual sampling methods, provide a three-way pipe connection system for bioreactor sampling, reduce the risk of operation and pollution, and A low risk of contamination is guaranteed during the cell culture cycle.

In order to solve the above technical problems, the utility model is realized through the following technical solutions: a three-way pipe connection system for bioreactor sampling, including a first silicone tube, a second silicone tube and a third silicone tube, the first A silicone tube, the second silicone tube and the third silicone tube are connected by a three-way connector, and the first silicone tube is connected to the sampling valve on the bioreactor through a leather tube joint and a clip; the third A sampling liquid outlet is provided on the silicone tube, and the sampling liquid outlet is a glass joint, and the glass joint includes a glass plug and a glass female seat, and the glass female seat is connected with a stop pipe.

Further, the first silicone tube, the second silicone tube and the third silicone tube are all provided with conduit clamps for controlling the flow of liquid.

In a preferred embodiment of the present utility model, the stop pipe is provided with strip-shaped stop clips.

Further, the end of the second silicone tube is provided with a first air filter.

As an improvement to the above solution, a second air filter is provided at the end of the stop pipe.

As an improvement of the above solution, the second silicone tube and the first air filter are suspended at a high place.

Compared with the prior art, the beneficial effect of the utility model is that the three-way pipeline connection system for bioreactor sampling of the utility model is formed by connecting three sections of silicone tubes with three-way connectors, one end of which is connected to the bioreactor , one end is used for sampling the liquid, and the other end is located at a higher place, which can be connected with the outside air through the air filter. The three-section pipeline is controlled by the pipe clamp to control the liquid flow. After sampling, the first silicone tube connected to the bioreactor can be clamped with a conduit clamp, and the conduit clamp on the second silicone tube connected to the air can be opened to discharge the liquid in the pipeline at the sampling outlet. The utility model can discharge the liquid at the sampling liquid end after sampling, reduce the risk of pollution during operation, prevent the liquid from dripping in the tube from affecting the operating environment, and have a low pollution risk in a long cell culture cycle, compared with the usual sampling pipeline , after the sampling is finished, the utility model can empty the glass joint of the sampling liquid end and the liquid in the pipeline, which is convenient for the burning and disinfection of the glass joint, and can reduce the biological pollution caused by the sampling operation in a long cell culture period. The risk of reactor contamination can also avoid the adverse effects of liquid dripping in the joint on the operating environment (such as ultra-clean workbench, etc.), which is worthy of popularization and application.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings of the embodiments will be briefly introduced below.

Fig. 1 is a schematic structural view of a three-way pipe connection system for bioreactor sampling in a preferred embodiment of the present invention.

Fig. 2 is a schematic structural view of the sampling outlet of the three-way pipe connection system used for bioreactor sampling in a preferred embodiment of the present invention.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

As shown in Fig. 1 and Fig. 2, the structure diagram of the three-way pipeline connection system for bioreactor sampling of the preferred embodiment of the present invention, the three-way pipeline connection system for bioreactor sampling of the present utility model includes the first A silicone tube 10, a second silicone tube 20 and a third silicone tube 30, the first silicone tube 10, the second silicone tube 20 and the third silicone tube 30 are connected by a tee connector 40, the first A silicone tube 10 is connected to the sampling valve 51 on the bioreactor 50 through a leather tube joint and a clamp 11; the third silicone tube 30 is provided with a sampling liquid outlet, and the sampling liquid outlet is a glass joint 60, The glass joint 60 includes a glass plug 61 and a glass female seat 62 , and the glass female seat 62 is connected with a stop tube 70 . In the utility model, the first silicone tube 10 , the second silicone tube 20 and the third silicone tube 30 are all provided with conduit clips 80 for controlling liquid flow.

Preferably, the stop tube 70 is provided with a strip-shaped stop clip 71 .

In the present utility model, the end of the second silicone tube 20 is provided with a first air filter 21, and the end of the stop pipe 70 is provided with a second air filter 72, and the first air filter 21 and the first air filter 72 are used The second air filter 72 is used to isolate external environmental pollution and ensure the sterile state inside the piping system.

In addition, the second silicone tube 20 and the first air filter 21 are suspended at high places.

The utility model is described in more detail below through the specific use process:

Before use, the pipeline connection system of the present invention and the bioreactor 50 have been sterilized by high pressure and are in a sterile state. The catheter clip 80 on the first silicone tube 10, the second silicone tube 20 and the third silicone tube 30 should be in a closed state when there is no operation, and the glass joint 60 should be docked and fixed with a rubber band when there is no operation. When in use, first open the sampling valve 51, in the ultra-clean workbench, untie the rubber band on the glass joint 60, burn and sterilize the glass joint 60 above the flame of the alcohol lamp, and separate it into a glass plug 61 and a glass female seat 62. The plug 61 is aimed at the bottle mouth of the waste liquid bottle, ready for draining. First open the catheter clamp 80 on the first silicone tube 10 and the third silicone tube 30 in sequence, and the liquid in the bioreactor 50 is drained into the waste liquid bottle through the first silicone tube 10 and the third silicone tube 30 due to the internal pressure of the container. Until the liquid flowing out from the bioreactor 50 is uniform, close the conduit clamp 80 on the third silicone tube 30 . Align the glass plug 61 with the mouth of the sampling container, open the conduit clip 80 on the third silicone tube 30 to take the sample, and then close the conduit clips 80 on the third silicone tube 30 and the first silicone tube 10 in sequence. Move the glass plug 61 to align with the mouth of the waste liquid bottle, open the catheter clamps 80 on the first silicone tube 10 and the second silicone tube 20 in sequence, and the liquid in the tubes is discharged due to atmospheric pressure. Empty the liquid in the third silicone tube 30, and then close the catheter clamp 80 on the third silicone tube 30 and the second silicone tube 20 in sequence. Burn the glass plug 61 and the glass female seat 62 on the alcohol lamp, and tie them up with rubber bands after they are connected. The strip-shaped stop clip 71 can prevent a small amount of liquid that may remain in the glass joint 60 from wetting and polluting the second air filter 72 .

The three-way pipeline connection system used for bioreactor sampling of the utility model is formed by connecting three sections of silicone tubes (the first silicone tube 10, the second silicone tube 20 and the third silicone tube 30) by a three-way tube connector 40, One end is connected to the bioreactor 50, one end is used for sampling liquid, and the other end is located at a higher place, which can communicate with the outside air through an air filter. After sampling, the first silicone tube 10 connected to the bioreactor 50 can be clamped with a conduit clamp 80, and the conduit clamp 80 on the second silicone tube 20 connected to the air can be opened to discharge the liquid in the pipeline at the sampling outlet. The utility model can discharge the liquid at the sampling liquid end after sampling, reduce the risk of pollution during operation, prevent the liquid from dripping in the tube from affecting the operating environment, and have a low pollution risk in a long cell culture cycle, compared with the usual sampling pipeline , after the sampling is finished, the utility model can empty the glass joint 60 of the sampling liquid end and the liquid in the pipeline, which is convenient for the burning and disinfection of the glass joint 60, and can reduce the existence of the sampling operation during a long cell culture period. It can reduce the risk of bioreactor contamination, and can also avoid the adverse effects of liquid dripping in the joint on the operating environment (such as ultra-clean workbench, etc.), which is worthy of popularization and application.

The above disclosures are only preferred embodiments of the present utility model, and of course the scope of rights of the present utility model cannot be limited by this, so the equivalent changes made according to the claims of the present utility model still belong to the scope covered by the present utility model .

Claims (6)

1. a kind of three-way pipeline connection system for bioreactor sampling it is characterised in that：Including the first silica gel tube, second Silica gel tube and the 3rd silica gel tube, pass through threeway pipe connections between described first silica gel tube, the second silica gel tube and the 3rd silica gel tube Connect, described first silica gel tube is connected with the sampling valve on bioreactor with clip by leather pipe joint；Described 3rd silica gel Pipe is provided with sampling liquid outlet, and described sampling liquid outlet is glass connector, and described glass connector includes glass plug and glass is female Seat, described glass female seat is connected with fluid stopping pipe.

2. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described The vessel clamp controlling liquid flowing is equipped with one silica gel tube, the second silica gel tube and the 3rd silica gel tube.

3. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described only Flow tube is provided with strip flow-stopping clip.

4. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described The end of second silica gel pipe is provided with the first air filter.

5. be used for as claimed in claim 1 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described only The end of flow tube is provided with the second air filter.

6. be used for as claimed in claim 4 the three-way pipeline connection system of bioreactor sampling it is characterised in that：Described Second silica gel pipe and the first air filter hang on eminence.